CmoCh11G012460 (gene) Cucurbita moschata (Rifu)

NameCmoCh11G012460
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
Description(Pectin methyl-esterase PER) (3.1.1.11) (Precursor)
LocationCmo_Chr11 : 7830333 .. 7832235 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTATGATGATGCTGCTGCTGAGAACCGGAAGCGCTACGCCATCATCGGCGTCTCGTCCATGCTACTCGTTGCGATGGTGGTGGCTGTGACCGTTGGAGTTAACCTAAACCAAGATGGAACGAGCGATCCGGCCACCGGAAAGAAGACTCATGAGATATCTTCTTCTATGAAAGCCATCAAAGCCATTTGCCGGCCTACCGATTACAAGCGAGAATGTGTTGATAGCCTTAAGGGGTCCGGAAAGTCGGACCCGAAGGAATTGGTTCAGGTTGGGTTCAAGGCTGCCATGAAACTCATCCAAGCCGCTGCTAAGAAATCTGTCACCTTAAGACAGATTGAGAAAGATCCTAGAGCTTCCCAAGCCCTTGCTGTGTGTAAGGAGCTCATGGATTATTCTGTTGACGAACTTAAATTCTCCATTCAAAAGCTTGGCGCCTTTGACATTAGTAAACTCGATGAGATGTTGATCGATATTAAGATCTGGCTCAGTGCCGTTATTACTTACCAGGTAATTCGTATTCTCCACTTTCAGCATAAGCTCTCGGTTGATTAAGAAGTTGATGGGTTATTGTTTCTTTTATTAGGAAACTTGCTTGGATGGGTTCGCGAACACGACAGGGGACGCATCGGAAAAGATGAAGAAGGCATTGAAGACATCGATGCGATTGAGCAGCAATGGGTTGGCGATGGTCACGGAGTTGTCCTCCATGTTGACGGAGTTGCAAATTCCAGGGATCAACCGACGACTTCTCAGCATTCCAGTTCTCGGGCACGAGGATTTCCCCGAGTGGGTCGACCCCGGTATGCAGCGTCTCCTGGCCGGTTCTCCGGCGAAGAGGAAGCCAAACATTGTGGTGGCTAAAGACGGAAGTGGGCAGTACAAAACCATTCAAGAGGCTATAAACAAAGTTCCCAAGAGGAAGAACAACGCAACTTTTGTAATCCATATCAAGCAAGGAGTGTACAAAGAATATGTTGACATTCAAAAAAAGCTAACCCATTTGATGCTCATTGGTGATGGCCCTAAAAAGACCATAATCACCGGAAATAAGAACTTCGTCGATGGCACGAACACGTTCAAGACCGCTACCGTTGGTGGGTTAATAATGCATTTTTTTAGAACATAAGGATGGACGACGTGGATCAATGATTTGTTGTTGTTGTCGTCGTTGTTGTTTGCAGCTGTTTCCGCAGAGCACTTCATGGCTAGAGACATCGGATTCGAGAACACGGCAGGACCACATAAGCACCAAGCCGTAGCATTACGAGTCCAAGCAGACAAAGTAGTATTCTTCAATTGCGAGATGCATGGATACCAAGACACCCTCTACGTACACACCATGCGTCAATTCTACCGGGACTGCACCATCTCCGGCACAATCGACTTCGTGTTCGGGGACGCAGCGGCCGTGTTCCAAAACTGCAGCTTCTTGGTGCGCAAGCCGCTGCCGAACCAACAAAACATCGTGACGGCGCACGGGCGGAAGGAGCGGAGGCAGCCCTCGGCCCTGATCATCCAGAATTCGAAGTTCAAGCCGGATGCGGATCTGGTGCCGGTGAAGAAGCAGTTCCGGTCGTACCTAGGGCGGCCGTGGAAGGAATATTCTAGAACGATCATAATGGAATCATACATAGATGACATAATAGATCCGGAAGGGTGGCTGCCGTGGGCAGGGGACTGGGGGCTGAGGACGTGCTTTTACACGGAGTTCAACAACTTCGGGCCGGGGTCGGGGAAGGCGAAGAGGGTGAAGTGGAGGGGAATCAAGAGCATTACGCCGCAGCACGCGGTGGACTTTACTCCGGGGAGGTTCATATTGGGGGATAGATGGATTAAAGCCACCGGAGTACCGTACGTTTCAGGGATGACCAAACCCAAGGCGGGAGTTGGTAATTGA

mRNA sequence

ATGGCTTATGATGATGCTGCTGCTGAGAACCGGAAGCGCTACGCCATCATCGGCGTCTCGTCCATGCTACTCGTTGCGATGGTGGTGGCTGTGACCGTTGGAGTTAACCTAAACCAAGATGGAACGAGCGATCCGGCCACCGGAAAGAAGACTCATGAGATATCTTCTTCTATGAAAGCCATCAAAGCCATTTGCCGGCCTACCGATTACAAGCGAGAATGTGTTGATAGCCTTAAGGGGTCCGGAAAGTCGGACCCGAAGGAATTGGTTCAGGTTGGGTTCAAGGCTGCCATGAAACTCATCCAAGCCGCTGCTAAGAAATCTGTCACCTTAAGACAGATTGAGAAAGATCCTAGAGCTTCCCAAGCCCTTGCTGTGTGTAAGGAGCTCATGGATTATTCTGTTGACGAACTTAAATTCTCCATTCAAAAGCTTGGCGCCTTTGACATTAGTAAACTCGATGAGATGTTGATCGATATTAAGATCTGGCTCAGTGCCGTTATTACTTACCAGGAAACTTGCTTGGATGGGTTCGCGAACACGACAGGGGACGCATCGGAAAAGATGAAGAAGGCATTGAAGACATCGATGCGATTGAGCAGCAATGGGTTGGCGATGGTCACGGAGTTGTCCTCCATGTTGACGGAGTTGCAAATTCCAGGGATCAACCGACGACTTCTCAGCATTCCAGTTCTCGGGCACGAGGATTTCCCCGAGTGGGTCGACCCCGGTATGCAGCGTCTCCTGGCCGGTTCTCCGGCGAAGAGGAAGCCAAACATTGTGGTGGCTAAAGACGGAAGTGGGCAGTACAAAACCATTCAAGAGGCTATAAACAAAGTTCCCAAGAGGAAGAACAACGCAACTTTTGTAATCCATATCAAGCAAGGAGTGTACAAAGAATATGTTGACATTCAAAAAAAGCTAACCCATTTGATGCTCATTGGTGATGGCCCTAAAAAGACCATAATCACCGGAAATAAGAACTTCGTCGATGGCACGAACACGTTCAAGACCGCTACCGTTGCTGTTTCCGCAGAGCACTTCATGGCTAGAGACATCGGATTCGAGAACACGGCAGGACCACATAAGCACCAAGCCGTAGCATTACGAGTCCAAGCAGACAAAGTAGTATTCTTCAATTGCGAGATGCATGGATACCAAGACACCCTCTACGTACACACCATGCGTCAATTCTACCGGGACTGCACCATCTCCGGCACAATCGACTTCGTGTTCGGGGACGCAGCGGCCGTGTTCCAAAACTGCAGCTTCTTGGTGCGCAAGCCGCTGCCGAACCAACAAAACATCGTGACGGCGCACGGGCGGAAGGAGCGGAGGCAGCCCTCGGCCCTGATCATCCAGAATTCGAAGTTCAAGCCGGATGCGGATCTGGTGCCGGTGAAGAAGCAGTTCCGGTCGTACCTAGGGCGGCCGTGGAAGGAATATTCTAGAACGATCATAATGGAATCATACATAGATGACATAATAGATCCGGAAGGGTGGCTGCCGTGGGCAGGGGACTGGGGGCTGAGGACGTGCTTTTACACGGAGTTCAACAACTTCGGGCCGGGGTCGGGGAAGGCGAAGAGGGTGAAGTGGAGGGGAATCAAGAGCATTACGCCGCAGCACGCGGTGGACTTTACTCCGGGGAGGTTCATATTGGGGGATAGATGGATTAAAGCCACCGGAGTACCGTACGTTTCAGGGATGACCAAACCCAAGGCGGGAGTTGGTAATTGA

Coding sequence (CDS)

ATGGCTTATGATGATGCTGCTGCTGAGAACCGGAAGCGCTACGCCATCATCGGCGTCTCGTCCATGCTACTCGTTGCGATGGTGGTGGCTGTGACCGTTGGAGTTAACCTAAACCAAGATGGAACGAGCGATCCGGCCACCGGAAAGAAGACTCATGAGATATCTTCTTCTATGAAAGCCATCAAAGCCATTTGCCGGCCTACCGATTACAAGCGAGAATGTGTTGATAGCCTTAAGGGGTCCGGAAAGTCGGACCCGAAGGAATTGGTTCAGGTTGGGTTCAAGGCTGCCATGAAACTCATCCAAGCCGCTGCTAAGAAATCTGTCACCTTAAGACAGATTGAGAAAGATCCTAGAGCTTCCCAAGCCCTTGCTGTGTGTAAGGAGCTCATGGATTATTCTGTTGACGAACTTAAATTCTCCATTCAAAAGCTTGGCGCCTTTGACATTAGTAAACTCGATGAGATGTTGATCGATATTAAGATCTGGCTCAGTGCCGTTATTACTTACCAGGAAACTTGCTTGGATGGGTTCGCGAACACGACAGGGGACGCATCGGAAAAGATGAAGAAGGCATTGAAGACATCGATGCGATTGAGCAGCAATGGGTTGGCGATGGTCACGGAGTTGTCCTCCATGTTGACGGAGTTGCAAATTCCAGGGATCAACCGACGACTTCTCAGCATTCCAGTTCTCGGGCACGAGGATTTCCCCGAGTGGGTCGACCCCGGTATGCAGCGTCTCCTGGCCGGTTCTCCGGCGAAGAGGAAGCCAAACATTGTGGTGGCTAAAGACGGAAGTGGGCAGTACAAAACCATTCAAGAGGCTATAAACAAAGTTCCCAAGAGGAAGAACAACGCAACTTTTGTAATCCATATCAAGCAAGGAGTGTACAAAGAATATGTTGACATTCAAAAAAAGCTAACCCATTTGATGCTCATTGGTGATGGCCCTAAAAAGACCATAATCACCGGAAATAAGAACTTCGTCGATGGCACGAACACGTTCAAGACCGCTACCGTTGCTGTTTCCGCAGAGCACTTCATGGCTAGAGACATCGGATTCGAGAACACGGCAGGACCACATAAGCACCAAGCCGTAGCATTACGAGTCCAAGCAGACAAAGTAGTATTCTTCAATTGCGAGATGCATGGATACCAAGACACCCTCTACGTACACACCATGCGTCAATTCTACCGGGACTGCACCATCTCCGGCACAATCGACTTCGTGTTCGGGGACGCAGCGGCCGTGTTCCAAAACTGCAGCTTCTTGGTGCGCAAGCCGCTGCCGAACCAACAAAACATCGTGACGGCGCACGGGCGGAAGGAGCGGAGGCAGCCCTCGGCCCTGATCATCCAGAATTCGAAGTTCAAGCCGGATGCGGATCTGGTGCCGGTGAAGAAGCAGTTCCGGTCGTACCTAGGGCGGCCGTGGAAGGAATATTCTAGAACGATCATAATGGAATCATACATAGATGACATAATAGATCCGGAAGGGTGGCTGCCGTGGGCAGGGGACTGGGGGCTGAGGACGTGCTTTTACACGGAGTTCAACAACTTCGGGCCGGGGTCGGGGAAGGCGAAGAGGGTGAAGTGGAGGGGAATCAAGAGCATTACGCCGCAGCACGCGGTGGACTTTACTCCGGGGAGGTTCATATTGGGGGATAGATGGATTAAAGCCACCGGAGTACCGTACGTTTCAGGGATGACCAAACCCAAGGCGGGAGTTGGTAATTGA
BLAST of CmoCh11G012460 vs. Swiss-Prot
Match: PME28_ARATH (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana GN=PME28 PE=2 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 1.9e-186
Identity = 318/572 (55.59%), Postives = 425/572 (74.30%), Query Frame = 1

Query: 3   YDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQ-DGTSDPATGKKTHEISSSMKAI 62
           YDD  A+ +KRY II +SS+LL++MVVAVT+GV++N+ D   D        EI++S+KAI
Sbjct: 5   YDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGD-------EEITTSVKAI 64

Query: 63  KAICRPTDYKRECVDSLKGSGK--SDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDPR 122
           K +C PTDYK  C D+L+   K  SDP ELV+  F A MK I   AKKS T+ +++KDPR
Sbjct: 65  KDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPR 124

Query: 123 ASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFA 182
           A  AL  CKELMDY++ EL  S ++LG F+  K+DE L+ ++IWLSA I++++TCLDGF 
Sbjct: 125 AKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQ 184

Query: 183 NTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDFP 242
            T G+A E +KKALKT+++L+ NGLAMVTE+S+ L ++QIP +N RRLLS      ++FP
Sbjct: 185 GTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLS------QEFP 244

Query: 243 EWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVY 302
            W+D   +RLL    ++ KP+IVVA+DGSGQYKTI EA+N VPK+K N TFV+HIK+G+Y
Sbjct: 245 SWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKK-NTTFVVHIKEGIY 304

Query: 303 KEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFENT 362
           KEYV + + +THL+ IGDGP KT+I+G+K++ DG  T+KTATVA+  +HF+A++I FENT
Sbjct: 305 KEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENT 364

Query: 363 AGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 422
           AG  KHQAVA+RV AD+ +F+NC+  GYQDTLY H+ RQFYRDCTISGTIDF+FGDAAAV
Sbjct: 365 AGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAV 424

Query: 423 FQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRP 482
           FQNC+ LVRKPL NQ   +TAHGRK+ R+ +  ++Q      + D + VK+Q ++YLGRP
Sbjct: 425 FQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRP 484

Query: 483 WKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIKS 542
           WKEYSRTIIM ++I D + PEGW PW G++GL T FY+E  N GPG+   KRV W GIK 
Sbjct: 485 WKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKK 544

Query: 543 ITPQHAVDFTPGRFILGDRWIKATGVPYVSGM 571
           ++ +  + FTP ++I GD WI   GVPY+ G+
Sbjct: 545 LSDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of CmoCh11G012460 vs. Swiss-Prot
Match: PME21_ARATH (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana GN=PME21 PE=2 SV=2)

HSP 1 Score: 641.3 bits (1653), Expect = 9.9e-183
Identity = 317/574 (55.23%), Postives = 425/574 (74.04%), Query Frame = 1

Query: 3   YDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQ-DGTSDPATGKKTHEISSSMKAI 62
           YDD + + ++RY +I +SS+LL++MVVAVTVGV+LN+ DG S     K   E+++S+KA+
Sbjct: 5   YDDES-KRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDS-----KGKAEVNASVKAV 64

Query: 63  KAICRPTDYKRECVDSLKGSGKS--DPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDPR 122
           K +C PTDY++ C D+L  +GK+  DP ELV+  F   MK I  AAKKS T+ +++KD R
Sbjct: 65  KDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSR 124

Query: 123 ASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFA 182
              AL  CKELMDY++DEL  S ++LG F+   LDE LI+++IWLSA I+++ETCL+GF 
Sbjct: 125 TRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQ 184

Query: 183 NTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDFP 242
            T G+A E MKKALKT++ L+ NGLA+++E+S+ + ++QIPG+N RRLL+      E FP
Sbjct: 185 GTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA------EGFP 244

Query: 243 EWVDPGMQRLLAGSPAKR--KPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQG 302
            WVD   ++LL  + A    KP+IVVA+DGSGQYKTI EA+  VPK K N TFV+HIK G
Sbjct: 245 SWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPK-KRNTTFVVHIKAG 304

Query: 303 VYKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFE 362
           +YKEYV + K ++HL+ IGDGP KTII+GNKN+ DG  T++TATVA+   +F+A++IGFE
Sbjct: 305 LYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFE 364

Query: 363 NTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAA 422
           NTAG  KHQAVA+RVQ+D+ +FFNC   GYQDTLY H+ RQF+RDCTISGTIDF+FGDAA
Sbjct: 365 NTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAA 424

Query: 423 AVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLG 482
           AVFQNC+ LVRKPLPNQ   +TAHGRK+ R+ +  + Q      + D + VK+  ++YLG
Sbjct: 425 AVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLG 484

Query: 483 RPWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGI 542
           RPWKEYSRTIIM ++I D + P+GW PW GD+GL+T FY+E  N GPGS  A RV W GI
Sbjct: 485 RPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGI 544

Query: 543 KSITPQHAVDFTPGRFILGDRWIKATGVPYVSGM 571
           K+++ +  + FTP ++I GD WI   GVPY +G+
Sbjct: 545 KTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of CmoCh11G012460 vs. Swiss-Prot
Match: PME58_ARATH (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana GN=PME58 PE=2 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 3.2e-149
Identity = 269/569 (47.28%), Postives = 378/569 (66.43%), Query Frame = 1

Query: 11  RKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKAIKAICRPTDY 70
           +K+  I GV + LLV MVVAV +  + N   +          +I ++  A++A+C PTDY
Sbjct: 9   KKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPV----QIKTATTAVEAVCAPTDY 68

Query: 71  KRECVDSLKGSG--KSDPKELVQVGFKAAMKLIQAAAKK-SVTLR-QIEKDPRASQALAV 130
           K  CV+SL  +    + P +L+++GF   ++ I+ + KK SV L  +   D     AL +
Sbjct: 69  KETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALEL 128

Query: 131 CKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANTTGDAS 190
           C++LM+ + D+LK  +     F I ++++ + D+++WLS  I YQ+TC+D F  T    S
Sbjct: 129 CEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLS 188

Query: 191 EKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-------RRLLSIPVLGHEDFPE 250
           + M+K  KTS  L+SNGLAM+T +S++L E  + G+        R+LLS      +  P 
Sbjct: 189 QDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLS----AEDGIPS 248

Query: 251 WVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVYK 310
           WV P  +RL+A +    K N+VVA DGSGQYKTI EA+N VPK  N   FVI+IKQGVY 
Sbjct: 249 WVGPNTRRLMA-TKGGVKANVVVAHDGSGQYKTINEALNAVPKA-NQKPFVIYIKQGVYN 308

Query: 311 EYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDG-TNTFKTATVAVSAEHFMARDIGFENT 370
           E VD+ KK+TH+  IGDGP KT ITG+ N+  G   T+ TATVA++ ++F A++IGFENT
Sbjct: 309 EKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENT 368

Query: 371 AGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 430
           AGP  HQAVALRV AD  VF+NC++ GYQDTLYVH+ RQF+RDCT+SGT+DF+FGD   V
Sbjct: 369 AGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVV 428

Query: 431 FQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRP 490
            QNC+ +VRKP+ +Q  ++TA GR ++R+ + L++QN     +   +PVK   ++YLGRP
Sbjct: 429 LQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRP 488

Query: 491 WKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIKS 550
           WKE+SRTIIM + IDD+IDP GWLPW GD+ L T +Y E+ N GPGS +A+RVKW GIK 
Sbjct: 489 WKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKK 548

Query: 551 ITPQHAVDFTPGRFILGDRWIKATGVPYV 568
           ++P+ A+ FTP RF+ G+ WI    VPY+
Sbjct: 549 LSPKQALRFTPARFLRGNLWIPPNRVPYM 567

BLAST of CmoCh11G012460 vs. Swiss-Prot
Match: PME23_ARATH (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana GN=PME23 PE=2 SV=3)

HSP 1 Score: 505.8 bits (1301), Expect = 6.5e-142
Identity = 259/571 (45.36%), Postives = 366/571 (64.10%), Query Frame = 1

Query: 9   ENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKAIKAICRPT 68
           + +K++ + G  S  LV MVV+V V        TS  +     + I  + KA++A+C PT
Sbjct: 6   DKKKKFIVAGSVSGFLVIMVVSVAVV-------TSKHSPKDDENHIRKTTKAVQAVCAPT 65

Query: 69  DYKRECVDSLKGSG--KSDPKELVQVGFKAAMKLIQAAAKKSV--TLRQIEKDPRASQAL 128
           D+K  CV+SL G+     DP +L+++GFK  +K I  + +K+      + +K+P A  A 
Sbjct: 66  DFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAF 125

Query: 129 AVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANTTGD 188
            +C++LM  ++D+LK  +     F + +++  + D+++WLS  I +Q+TC+D F     +
Sbjct: 126 ELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKSN 185

Query: 189 ASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRRLLSIP--VLGHED-FPEWV 248
             + M K  KTS  LSSN LAMVT +S+++    + G+   L      +L  ED  P WV
Sbjct: 186 LMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTEDSIPTWV 245

Query: 249 DPGMQRLLA---GSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVY 308
            P  +RL+A   G P   K N VVA+DG+GQ+KTI +A+N VPK  N   F+IHIK+G+Y
Sbjct: 246 GPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKG-NKVPFIIHIKEGIY 305

Query: 309 KEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDG-TNTFKTATVAVSAEHFMARDIGFEN 368
           KE V + KK+ H+  IGDGP KT+ITG+ NF  G   TF TAT+ +  +HF A++IG EN
Sbjct: 306 KEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGIEN 365

Query: 369 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 428
           TAGP   QAVALRV AD  VF +C++ G+QDTLYVH+ RQFYRDCT+SGT+DF+FGDA  
Sbjct: 366 TAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDAKC 425

Query: 429 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 488
           + QNC  +VRKP   Q  +VTA GR   R+ + L++       D   +P+K   ++YLGR
Sbjct: 426 ILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKAYLGR 485

Query: 489 PWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIK 548
           PWKE+SRTIIM++ IDD+IDP GWLPW+GD+ L+T +Y E  N GPGS +A+RVKW GIK
Sbjct: 486 PWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPGIK 545

Query: 549 SITPQHAVDFTPGRFILGDRWIKATGVPYVS 569
            +TPQ A+ +T  RF+ GD WI  T VPY +
Sbjct: 546 KLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of CmoCh11G012460 vs. Swiss-Prot
Match: PME_MEDSA (Pectinesterase/pectinesterase inhibitor OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 2.2e-126
Identity = 228/454 (50.22%), Postives = 302/454 (66.52%), Query Frame = 1

Query: 124 LAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANTTG 183
           + +C E++DY+VD +  S+  L  FD  KL E   D+K+WL+  +++Q+TCLDGFANTT 
Sbjct: 1   MEICNEVLDYAVDGIHKSVGTLDQFDFHKLSEYAFDLKVWLTGTLSHQQTCLDGFANTTT 60

Query: 184 DASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIP--GINRRLLSIPVLGHEDFPEWV 243
            A E M K LKTSM LSSN + M+  +S +L         ++RRLLS      +  P WV
Sbjct: 61  KAGETMTKVLKTSMELSSNAIDMMDAVSRILKGFDTSQYSVSRRLLS-----DDGIPSWV 120

Query: 244 DPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVYKEY 303
           + G +RLLAG     +PN VVA+DGSGQ+KT+ +A+  VP  KN   FVIH+K GVYKE 
Sbjct: 121 NDGHRRLLAGGNV--QPNAVVAQDGSGQFKTLTDALKTVPP-KNAVPFVIHVKAGVYKET 180

Query: 304 VDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFENTAGP 363
           V++ K++ ++ +IGDGP KT  TG+ N+ DG NT+ TAT  V+  +FMA+DIGFENTAG 
Sbjct: 181 VNVAKEMNYVTVIGDGPTKTKFTGSLNYADGINTYNTATFGVNGANFMAKDIGFENTAGT 240

Query: 364 HKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAVFQN 423
            KHQAVALRV AD+ +F+NC+M G+QDTLYV + RQFYRDC+ISGTIDFVFG+   VFQN
Sbjct: 241 GKHQAVALRVTADQAIFYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGERFGVFQN 300

Query: 424 CSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRPWKE 483
           C  + R P   QQ +VTA GR+++   SAL+ Q+S F  +  L  V  +  SYLGRPWK+
Sbjct: 301 CKLVCRLPAKGQQCLVTAGGREKQNSASALVFQSSHFTGEPALTSVTPKV-SYLGRPWKQ 360

Query: 484 YSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIKSITP 543
           YS+ +IM+S ID I  PEG++PW G     TC Y E+NN GPG+    RVKW G+K +T 
Sbjct: 361 YSKVVIMDSTIDAIFVPEGYMPWMGSAFKETCTYYEYNNKGPGADTNLRVKWHGVKVLTS 420

Query: 544 QHAVDFTPGRFI------LGDRWIKATGVPYVSG 570
             A ++ PG+F         D WI  +GVPY  G
Sbjct: 421 NVAAEYYPGKFFEIVNATARDTWIVKSGVPYSLG 445

BLAST of CmoCh11G012460 vs. TrEMBL
Match: A0A0A0LYF5_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_1G569220 PE=4 SV=1)

HSP 1 Score: 983.0 bits (2540), Expect = 1.5e-283
Identity = 479/575 (83.30%), Postives = 534/575 (92.87%), Query Frame = 1

Query: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKA 60
           M Y D  ++N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQD TSDPATG K+HEISSSMKA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICRPTDYKRECVDSLKGSGK--SDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDP 120
           IKAIC+PTDYK+ECV SLK +G   SDPKELVQ GFKAAMKLIQAAA KSV L Q+EKDP
Sbjct: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120

Query: 121 RASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180
           RAS+ALA CKELMD+++DELK+S+ KLG FDISKLDEMLIDI+IWLSA ITYQETCLDGF
Sbjct: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ANTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRR-LLSIPVLGHEDF 240
           ANTTG+A+EKMKKALKTSM+LSSNGLAMV+++SSML+ELQIPGI+RR LL IPVLGH+D+
Sbjct: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300
           P+W +PGM+RLLA   +K KPN+VVAKDGSGQ+KTIQEAI++VPKRKNNAT+VIHIK GV
Sbjct: 241 PDWANPGMRRLLAAG-SKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGV 300

Query: 301 YKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFEN 360
           Y+EYV ++K LTHLMLIGDGPKKTIITGNKNF+DGT TFKTATVAV+AEHFMARDIGFEN
Sbjct: 301 YQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFEN 360

Query: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420
           TAGP KHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDCT+SGTIDF+FGDAAA
Sbjct: 361 TAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAA 420

Query: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480
           +FQ+C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQN  FKP ADLVPV+KQFRS+LGR
Sbjct: 421 IFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGR 480

Query: 481 PWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIK 540
           PWKEYSRTIIMESYI D+I PEGWLPWAGDWGLRTCFYTE+NN+GPGS K+KRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIK 540

Query: 541 SITPQHAVDFTPGRFILGDRWIKATGVPYVSGMTK 573
           +ITPQHAVDFTPGRF+ GDRWIK TGVPYVSG+T+
Sbjct: 541 NITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTR 574

BLAST of CmoCh11G012460 vs. TrEMBL
Match: W9S2R1_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_001919 PE=4 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 2.2e-221
Identity = 382/581 (65.75%), Postives = 466/581 (80.21%), Query Frame = 1

Query: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKK--THEISSSM 60
           M+Y D   E RKRYA+IG+SS+LLVAMV+AVTVGV+LN +G S PA+  K  +HE+S+SM
Sbjct: 1   MSYGDDG-EKRKRYAVIGISSVLLVAMVIAVTVGVSLN-NGQSSPASSNKDGSHEVSASM 60

Query: 61  KAIKAICRPTDYKRECVDSL--KGSGKSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEK 120
           KAIK+IC+PTDYK EC++SL  +    +DPK+L+Q GFK AMK +  AAKKSV L+ +EK
Sbjct: 61  KAIKSICQPTDYKEECINSLSSESGNTTDPKKLIQAGFKVAMKYLSEAAKKSVVLQDLEK 120

Query: 121 DPRASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLD 180
           DPRAS+AL  CK+LM+ S+D+LK S+ KLG FDISK D+ML+D+KIWLSA +TYQETCLD
Sbjct: 121 DPRASKALGHCKQLMENSIDDLKHSVDKLGEFDISKFDQMLMDLKIWLSATVTYQETCLD 180

Query: 181 GFANTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRRLLS------IP 240
           GF NTTG+A+EKMKKALKTSMRLSSNGL MV+E+SS+ ++LQ+  + RRL S      +P
Sbjct: 181 GFQNTTGEAAEKMKKALKTSMRLSSNGLTMVSEISSVFSDLQLSSMGRRLFSDEEESELP 240

Query: 241 VLGHEDFPEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFV 300
           VLGH D  EW   G++RL+A  P K KPNIVVAKDGSG+Y TI EA+  +P+R  N TFV
Sbjct: 241 VLGHSDEAEWFGGGVRRLMAVHPTKVKPNIVVAKDGSGKYTTINEALKDIPRRSEN-TFV 300

Query: 301 IHIKQGVYKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMA 360
           I+IK+GVYKEYV I++ +T+L++IGDG  KT ITG+KNF+DG  T  TATV V  +HF A
Sbjct: 301 IYIKEGVYKEYVTIERNMTNLLIIGDGANKTRITGDKNFIDGFPTSLTATVVVLGDHFRA 360

Query: 361 RDIGFENTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDF 420
           RDIGFENTAG HKHQAVALRV AD+ +F+ C M GYQDTLY H  RQFYRDC +SGTIDF
Sbjct: 361 RDIGFENTAGAHKHQAVALRVNADEAIFYRCAMDGYQDTLYTHAKRQFYRDCNVSGTIDF 420

Query: 421 VFGDAAAVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQ 480
           VFGDAAAVFQNC F+VR+P+ NQQ IVTA GRKERRQPSA+IIQN  F  D D  PV+ +
Sbjct: 421 VFGDAAAVFQNCRFIVRRPMDNQQCIVTAQGRKERRQPSAIIIQNGTFVADPDYYPVRFE 480

Query: 481 FRSYLGRPWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGK-AK 540
             +YLGRPWKE+SRTIIMES+IDD+I P GWLPWAGD+GLRTCFYTEF N GPG+ + ++
Sbjct: 481 LPAYLGRPWKEFSRTIIMESFIDDLIQPRGWLPWAGDFGLRTCFYTEFGNRGPGAVEPSR 540

Query: 541 RVKWRGIKSITPQHAVDFTPGRFILGDRWIKATGVPYVSGM 571
           R KW+G+K+IT +HA DFTPG F  GD WIK TG+PYVSGM
Sbjct: 541 RAKWKGVKNITAKHARDFTPGSFFKGDWWIKRTGIPYVSGM 578

BLAST of CmoCh11G012460 vs. TrEMBL
Match: A0A067JV77_JATCU (Pectinesterase OS=Jatropha curcas GN=JCGZ_23282 PE=4 SV=1)

HSP 1 Score: 742.3 bits (1915), Expect = 4.6e-211
Identity = 369/582 (63.40%), Postives = 451/582 (77.49%), Query Frame = 1

Query: 4   DDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKK---THEISSSMKA 63
           DDA   N KR AIIG+SS+LLVAMVVAVT+GV  +++G S+  TGK    T EIS+SMKA
Sbjct: 3   DDA---NNKRLAIIGISSILLVAMVVAVTIGVGFSENGESEDITGKSHKSTGEISASMKA 62

Query: 64  IKAICRPTDYKRECVDSLKGSG--KSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDP 123
           IK+IC+P DYK+ C  SL+ S    +DPKEL++  FK A K I+ AAKKS TL ++EKDP
Sbjct: 63  IKSICQPADYKKTCEKSLEKSAGNTTDPKELIKAAFKIAQKKIREAAKKSATLEELEKDP 122

Query: 124 RASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 183
           R   AL  CKELMD S+ EL+ S  K+  FDI+ LD+ + D++ WLSAVITY+ TCLDGF
Sbjct: 123 RTRDALQSCKELMDMSISELQNSFDKVADFDITMLDDFMADLRTWLSAVITYEGTCLDGF 182

Query: 184 ANTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRRLLSIP-VLGHED- 243
            NTT DA EKMKKA+K ++ +S+NGL ++  LSS L +LQI G+NRRLL    VLGH+D 
Sbjct: 183 ENTTSDAGEKMKKAMKAAIEMSTNGLDIIDGLSSALADLQITGVNRRLLQEDEVLGHDDV 242

Query: 244 ---FPEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNAT--FVI 303
              FP WVD G +RLLA  P+K KPN+ VAKDGSG +KTI+ A++ +PK K  +T  FV+
Sbjct: 243 PSKFPSWVDAGTRRLLAAPPSKIKPNLTVAKDGSGDFKTIRGALDSIPKPKKGSTQTFVL 302

Query: 304 HIKQGVYKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMAR 363
           +IK GVY+EYV+  K + +LM+IGDGP+KT ITGNKNFVDG NT+ TATV V  ++F+A+
Sbjct: 303 YIKAGVYQEYVEFTKSMDYLMVIGDGPEKTRITGNKNFVDGVNTYHTATVVVLGDNFIAK 362

Query: 364 DIGFENTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFV 423
           +IGFEN+AG  KHQAVALRV AD  VF+NC M GYQDTLY H  RQFYR+C+ISGTIDFV
Sbjct: 363 NIGFENSAGAIKHQAVALRVSADFAVFYNCAMDGYQDTLYTHAKRQFYRECSISGTIDFV 422

Query: 424 FGDAAAVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQF 483
           FGDA  VFQNC FLVRKPL NQQ IVTA GRK+RRQPS +IIQN  F    +LVPVKKQ+
Sbjct: 423 FGDAPVVFQNCKFLVRKPLENQQCIVTAQGRKQRRQPSGIIIQNGTFTAHPELVPVKKQY 482

Query: 484 RSYLGRPWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRV 543
           +SYLGRPWK++SRTIIMESYIDDII+PEGWLPW G +GL+TC+YTEFNN+GPGS K  RV
Sbjct: 483 KSYLGRPWKQFSRTIIMESYIDDIIEPEGWLPWFGTFGLKTCWYTEFNNYGPGSDKKARV 542

Query: 544 KWRGIKSITPQHAVDFTPGRFILGDRWIKATGVPYVSGMTKP 574
           KW GIK+I  QHA+DFTPGRF+ GD WIK TG+PY   +T+P
Sbjct: 543 KWNGIKTIDRQHALDFTPGRFLRGDTWIKPTGIPYSPFLTRP 581

BLAST of CmoCh11G012460 vs. TrEMBL
Match: B9RFE0_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_1433930 PE=4 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 1.5e-209
Identity = 362/577 (62.74%), Postives = 454/577 (78.68%), Query Frame = 1

Query: 8   AENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTH---EISSSMKAIKAI 67
           ++ +KR AIIGVSS LLVAMVVAVTV +  N DG S+     K H   E+S+SMKAIK +
Sbjct: 7   SKRKKRLAIIGVSSFLLVAMVVAVTVSIGFNNDGESEDDINGKNHKSKEVSASMKAIKTL 66

Query: 68  CRPTDYKRECVDSL-KGSGKS-DPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDPRASQ 127
           C+PT YK+ C  SL K +G + DPKEL+++ FK A K I +A+KKS+TL ++EKDPR   
Sbjct: 67  CQPTYYKQTCERSLAKSAGNTTDPKELIKIAFKLAEKQIDSASKKSLTLLELEKDPRTRG 126

Query: 128 ALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANTT 187
           AL  CKELM  S++EL+ S++K+  FD S+LDE++ DIK WLSA ITY+ETCLD F NTT
Sbjct: 127 ALNSCKELMTMSINELRSSLEKVADFDFSQLDELMADIKTWLSAAITYEETCLDAFENTT 186

Query: 188 GDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRRLLS--IPVLGHED---- 247
            +A EKMKKALKT+M +SSNGL +V+ +SS+LT+LQIPG++RRLL   IPV GH D    
Sbjct: 187 TNAGEKMKKALKTAMEMSSNGLDIVSGISSVLTDLQIPGVSRRLLQDDIPVAGHGDISQA 246

Query: 248 FPEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQG 307
           FP W+DPG +RLL+  P+  KP++VVAKDGSG YKTI EA+ ++PK K+N TFV++IK+G
Sbjct: 247 FPAWIDPGTRRLLSAPPSNIKPDLVVAKDGSGDYKTILEALPQIPK-KSNETFVLYIKEG 306

Query: 308 VYKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFE 367
           +Y+EYV+  + +T+L++IGDGP KT ITG+KNFVDG NT++TATVAV  ++F+AR+IGFE
Sbjct: 307 IYEEYVEFNRSMTNLVVIGDGPDKTRITGSKNFVDGINTYRTATVAVIGDNFVARNIGFE 366

Query: 368 NTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAA 427
           N+AG  KHQAVALRV +D  VF+NC M GYQDTLY H  RQFYRDCT+SGTIDFVFGDA 
Sbjct: 367 NSAGAIKHQAVALRVSSDYAVFYNCSMDGYQDTLYTHAKRQFYRDCTVSGTIDFVFGDAP 426

Query: 428 AVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLG 487
            VFQNC+FLVRKPL NQQ IVTA GRK RRQPSA+IIQNS      +L PVK Q++SYLG
Sbjct: 427 VVFQNCTFLVRKPLENQQCIVTAQGRKARRQPSAIIIQNSTITAHPELEPVKDQYKSYLG 486

Query: 488 RPWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGI 547
           RPWKE+SRTIIME++IDD+I PEGW PW G +GL+TC+Y E+NN+GPGS    RVKW GI
Sbjct: 487 RPWKEFSRTIIMETFIDDLIQPEGWSPWFGSFGLKTCWYGEYNNYGPGSDMKNRVKWNGI 546

Query: 548 KSITPQHAVDFTPGRFILGDRWIKATGVPYVSGMTKP 574
           K ++ QHA+DFTPGRF+ GD WIK TGVPY   +T+P
Sbjct: 547 KPVSRQHAIDFTPGRFLRGDSWIKPTGVPYAPYLTRP 582

BLAST of CmoCh11G012460 vs. TrEMBL
Match: D7U9X3_VITVI (Pectinesterase OS=Vitis vinifera GN=VIT_14s0060g01950 PE=4 SV=1)

HSP 1 Score: 711.1 bits (1834), Expect = 1.1e-201
Identity = 353/567 (62.26%), Postives = 440/567 (77.60%), Query Frame = 1

Query: 11  RKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKAIKAICRPTDY 70
           +K+ AIIG SS+ LVAMV+AV   V+     +SD    +K  EISSSMKAI+AIC+PTDY
Sbjct: 13  KKKLAIIGFSSIFLVAMVIAVIASVS---SSSSDSGNAQK-QEISSSMKAIQAICQPTDY 72

Query: 71  KRECVDSL--KGSGKSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDPRASQALAVCK 130
           K  CV+SL  K    +DPK+LVQ  F +AM+ + AAAK S  L+++ KDPRASQAL  C+
Sbjct: 73  KDACVNSLTSKAGNTTDPKDLVQAAFASAMEHLSAAAKNSTLLQELNKDPRASQALQNCE 132

Query: 131 ELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANTTGDASEK 190
           +L++Y++D+LK S  ++G FD SK+D ++ DIKIWLSAVITYQETCLDGF NTTGDA EK
Sbjct: 133 DLVNYAIDDLKKSFNQVGDFDYSKMDNIIADIKIWLSAVITYQETCLDGFENTTGDAGEK 192

Query: 191 MKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRRLLSIPVLG-----HEDFPEWVDP 250
           M++ LKTSM LSSNGLA+V E+SS+L+ LQ+  +NRRLLS           ++FP W   
Sbjct: 193 MRQILKTSMELSSNGLAIVGEVSSILSNLQLANLNRRLLSDDPADPDNHIDDEFPYWSHS 252

Query: 251 GMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVYKEYVD 310
             ++LL  + ++ KPN+ VAKDGSG +KTI EAI ++PK  N  TF+++IK+G+Y+E V 
Sbjct: 253 EGRKLLQANVSELKPNLTVAKDGSGDFKTINEAIRQLPKFSNQ-TFILYIKKGIYEEQVQ 312

Query: 311 IQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFENTAGPHK 370
           I K  T+LM++GDGP KT ITG+ NFVDGT TFKTATVAV  + F+A+ IGFEN+AG  K
Sbjct: 313 INKTFTNLMMVGDGPTKTKITGSLNFVDGTPTFKTATVAVLGDGFIAKGIGFENSAGAAK 372

Query: 371 HQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAVFQNCS 430
           HQAVALRVQ+D+ +F+NC+M GYQDTLY HT RQFYRDCTISGTIDF+FGDAA +FQNC+
Sbjct: 373 HQAVALRVQSDRSIFYNCQMDGYQDTLYTHTKRQFYRDCTISGTIDFIFGDAAVIFQNCT 432

Query: 431 FLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRPWKEYS 490
           F+VRKPL NQQ IVTA GRKERRQPSA+IIQNS F  D +  P + + +SYLGRPWKE+S
Sbjct: 433 FVVRKPLDNQQCIVTAQGRKERRQPSAIIIQNSTFTADPEYYPYRNELKSYLGRPWKEFS 492

Query: 491 RTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIKSITPQH 550
           RTIIMESYI+D+I P GWLPWAGD+ LRTCFYTEF N GPG+    RVKWRGIK+I P H
Sbjct: 493 RTIIMESYIEDLIQPSGWLPWAGDFALRTCFYTEFRNRGPGAKTHDRVKWRGIKTIKPSH 552

Query: 551 AVDFTPGRFILGDRWIKATGVPYVSGM 571
           A+DF PGRF+ GDRWI +TGVPY SG+
Sbjct: 553 AIDFAPGRFLSGDRWIPSTGVPYNSGL 574

BLAST of CmoCh11G012460 vs. TAIR10
Match: AT5G27870.1 (AT5G27870.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 653.7 bits (1685), Expect = 1.1e-187
Identity = 318/572 (55.59%), Postives = 425/572 (74.30%), Query Frame = 1

Query: 3   YDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQ-DGTSDPATGKKTHEISSSMKAI 62
           YDD  A+ +KRY II +SS+LL++MVVAVT+GV++N+ D   D        EI++S+KAI
Sbjct: 5   YDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGD-------EEITTSVKAI 64

Query: 63  KAICRPTDYKRECVDSLKGSGK--SDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDPR 122
           K +C PTDYK  C D+L+   K  SDP ELV+  F A MK I   AKKS T+ +++KDPR
Sbjct: 65  KDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPR 124

Query: 123 ASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFA 182
           A  AL  CKELMDY++ EL  S ++LG F+  K+DE L+ ++IWLSA I++++TCLDGF 
Sbjct: 125 AKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQ 184

Query: 183 NTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDFP 242
            T G+A E +KKALKT+++L+ NGLAMVTE+S+ L ++QIP +N RRLLS      ++FP
Sbjct: 185 GTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLS------QEFP 244

Query: 243 EWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVY 302
            W+D   +RLL    ++ KP+IVVA+DGSGQYKTI EA+N VPK+K N TFV+HIK+G+Y
Sbjct: 245 SWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKK-NTTFVVHIKEGIY 304

Query: 303 KEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFENT 362
           KEYV + + +THL+ IGDGP KT+I+G+K++ DG  T+KTATVA+  +HF+A++I FENT
Sbjct: 305 KEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENT 364

Query: 363 AGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 422
           AG  KHQAVA+RV AD+ +F+NC+  GYQDTLY H+ RQFYRDCTISGTIDF+FGDAAAV
Sbjct: 365 AGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAV 424

Query: 423 FQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRP 482
           FQNC+ LVRKPL NQ   +TAHGRK+ R+ +  ++Q      + D + VK+Q ++YLGRP
Sbjct: 425 FQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRP 484

Query: 483 WKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIKS 542
           WKEYSRTIIM ++I D + PEGW PW G++GL T FY+E  N GPG+   KRV W GIK 
Sbjct: 485 WKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKK 544

Query: 543 ITPQHAVDFTPGRFILGDRWIKATGVPYVSGM 571
           ++ +  + FTP ++I GD WI   GVPY+ G+
Sbjct: 545 LSDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of CmoCh11G012460 vs. TAIR10
Match: AT3G05610.1 (AT3G05610.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 641.3 bits (1653), Expect = 5.6e-184
Identity = 317/574 (55.23%), Postives = 425/574 (74.04%), Query Frame = 1

Query: 3   YDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQ-DGTSDPATGKKTHEISSSMKAI 62
           YDD + + ++RY +I +SS+LL++MVVAVTVGV+LN+ DG S     K   E+++S+KA+
Sbjct: 5   YDDES-KRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDS-----KGKAEVNASVKAV 64

Query: 63  KAICRPTDYKRECVDSLKGSGKS--DPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDPR 122
           K +C PTDY++ C D+L  +GK+  DP ELV+  F   MK I  AAKKS T+ +++KD R
Sbjct: 65  KDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSR 124

Query: 123 ASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFA 182
              AL  CKELMDY++DEL  S ++LG F+   LDE LI+++IWLSA I+++ETCL+GF 
Sbjct: 125 TRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQ 184

Query: 183 NTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDFP 242
            T G+A E MKKALKT++ L+ NGLA+++E+S+ + ++QIPG+N RRLL+      E FP
Sbjct: 185 GTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA------EGFP 244

Query: 243 EWVDPGMQRLLAGSPAKR--KPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQG 302
            WVD   ++LL  + A    KP+IVVA+DGSGQYKTI EA+  VPK K N TFV+HIK G
Sbjct: 245 SWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPK-KRNTTFVVHIKAG 304

Query: 303 VYKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFE 362
           +YKEYV + K ++HL+ IGDGP KTII+GNKN+ DG  T++TATVA+   +F+A++IGFE
Sbjct: 305 LYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFE 364

Query: 363 NTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAA 422
           NTAG  KHQAVA+RVQ+D+ +FFNC   GYQDTLY H+ RQF+RDCTISGTIDF+FGDAA
Sbjct: 365 NTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAA 424

Query: 423 AVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLG 482
           AVFQNC+ LVRKPLPNQ   +TAHGRK+ R+ +  + Q      + D + VK+  ++YLG
Sbjct: 425 AVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLG 484

Query: 483 RPWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGI 542
           RPWKEYSRTIIM ++I D + P+GW PW GD+GL+T FY+E  N GPGS  A RV W GI
Sbjct: 485 RPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGI 544

Query: 543 KSITPQHAVDFTPGRFILGDRWIKATGVPYVSGM 571
           K+++ +  + FTP ++I GD WI   GVPY +G+
Sbjct: 545 KTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of CmoCh11G012460 vs. TAIR10
Match: AT5G49180.1 (AT5G49180.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 530.0 bits (1364), Expect = 1.8e-150
Identity = 269/569 (47.28%), Postives = 378/569 (66.43%), Query Frame = 1

Query: 11  RKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKAIKAICRPTDY 70
           +K+  I GV + LLV MVVAV +  + N   +          +I ++  A++A+C PTDY
Sbjct: 9   KKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPV----QIKTATTAVEAVCAPTDY 68

Query: 71  KRECVDSLKGSG--KSDPKELVQVGFKAAMKLIQAAAKK-SVTLR-QIEKDPRASQALAV 130
           K  CV+SL  +    + P +L+++GF   ++ I+ + KK SV L  +   D     AL +
Sbjct: 69  KETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALEL 128

Query: 131 CKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANTTGDAS 190
           C++LM+ + D+LK  +     F I ++++ + D+++WLS  I YQ+TC+D F  T    S
Sbjct: 129 CEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLS 188

Query: 191 EKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-------RRLLSIPVLGHEDFPE 250
           + M+K  KTS  L+SNGLAM+T +S++L E  + G+        R+LLS      +  P 
Sbjct: 189 QDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLS----AEDGIPS 248

Query: 251 WVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVYK 310
           WV P  +RL+A +    K N+VVA DGSGQYKTI EA+N VPK  N   FVI+IKQGVY 
Sbjct: 249 WVGPNTRRLMA-TKGGVKANVVVAHDGSGQYKTINEALNAVPKA-NQKPFVIYIKQGVYN 308

Query: 311 EYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDG-TNTFKTATVAVSAEHFMARDIGFENT 370
           E VD+ KK+TH+  IGDGP KT ITG+ N+  G   T+ TATVA++ ++F A++IGFENT
Sbjct: 309 EKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENT 368

Query: 371 AGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 430
           AGP  HQAVALRV AD  VF+NC++ GYQDTLYVH+ RQF+RDCT+SGT+DF+FGD   V
Sbjct: 369 AGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVV 428

Query: 431 FQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRP 490
            QNC+ +VRKP+ +Q  ++TA GR ++R+ + L++QN     +   +PVK   ++YLGRP
Sbjct: 429 LQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRP 488

Query: 491 WKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIKS 550
           WKE+SRTIIM + IDD+IDP GWLPW GD+ L T +Y E+ N GPGS +A+RVKW GIK 
Sbjct: 489 WKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKK 548

Query: 551 ITPQHAVDFTPGRFILGDRWIKATGVPYV 568
           ++P+ A+ FTP RF+ G+ WI    VPY+
Sbjct: 549 LSPKQALRFTPARFLRGNLWIPPNRVPYM 567

BLAST of CmoCh11G012460 vs. TAIR10
Match: AT3G06830.1 (AT3G06830.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 505.8 bits (1301), Expect = 3.6e-143
Identity = 259/571 (45.36%), Postives = 366/571 (64.10%), Query Frame = 1

Query: 9   ENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKAIKAICRPT 68
           + +K++ + G  S  LV MVV+V V        TS  +     + I  + KA++A+C PT
Sbjct: 6   DKKKKFIVAGSVSGFLVIMVVSVAVV-------TSKHSPKDDENHIRKTTKAVQAVCAPT 65

Query: 69  DYKRECVDSLKGSG--KSDPKELVQVGFKAAMKLIQAAAKKSV--TLRQIEKDPRASQAL 128
           D+K  CV+SL G+     DP +L+++GFK  +K I  + +K+      + +K+P A  A 
Sbjct: 66  DFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAF 125

Query: 129 AVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANTTGD 188
            +C++LM  ++D+LK  +     F + +++  + D+++WLS  I +Q+TC+D F     +
Sbjct: 126 ELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKSN 185

Query: 189 ASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRRLLSIP--VLGHED-FPEWV 248
             + M K  KTS  LSSN LAMVT +S+++    + G+   L      +L  ED  P WV
Sbjct: 186 LMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTEDSIPTWV 245

Query: 249 DPGMQRLLA---GSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVY 308
            P  +RL+A   G P   K N VVA+DG+GQ+KTI +A+N VPK  N   F+IHIK+G+Y
Sbjct: 246 GPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKG-NKVPFIIHIKEGIY 305

Query: 309 KEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDG-TNTFKTATVAVSAEHFMARDIGFEN 368
           KE V + KK+ H+  IGDGP KT+ITG+ NF  G   TF TAT+ +  +HF A++IG EN
Sbjct: 306 KEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGIEN 365

Query: 369 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 428
           TAGP   QAVALRV AD  VF +C++ G+QDTLYVH+ RQFYRDCT+SGT+DF+FGDA  
Sbjct: 366 TAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDAKC 425

Query: 429 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 488
           + QNC  +VRKP   Q  +VTA GR   R+ + L++       D   +P+K   ++YLGR
Sbjct: 426 ILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKAYLGR 485

Query: 489 PWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIK 548
           PWKE+SRTIIM++ IDD+IDP GWLPW+GD+ L+T +Y E  N GPGS +A+RVKW GIK
Sbjct: 486 PWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPGIK 545

Query: 549 SITPQHAVDFTPGRFILGDRWIKATGVPYVS 569
            +TPQ A+ +T  RF+ GD WI  T VPY +
Sbjct: 546 KLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of CmoCh11G012460 vs. TAIR10
Match: AT2G26450.1 (AT2G26450.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 427.6 bits (1098), Expect = 1.3e-119
Identity = 219/533 (41.09%), Postives = 328/533 (61.54%), Query Frame = 1

Query: 45  PATGKKTHEISSSMKAIKAICRPTDYKRECVDSLK-----GSGKSDPKELVQVGFKAAMK 104
           P +  ++ +     K I+ +C  T Y + C  +LK     G    +P   ++   +A  +
Sbjct: 93  PVSAAQSVKPGQGDKIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNE 152

Query: 105 LIQAAAKKSVTLRQIEKDPRASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLID 164
            +    +K ++L+   +D +   A+  CK L++ + +E   S+ K+   +++  ++++ D
Sbjct: 153 DLDLVLEKVLSLKTENQDDK--DAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPD 212

Query: 165 IKIWLSAVITYQETCLDGFANTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQI 224
           ++ WLSAV++YQETCLDGF    G+   ++K ++ +S  L+SN LA++   +  L+ + +
Sbjct: 213 LESWLSAVMSYQETCLDGFEE--GNLKSEVKTSVNSSQVLTSNSLALIKTFTENLSPV-M 272

Query: 225 PGINRRLLSIPVLGHEDFPEWVDPGMQRLLAGSPAKR-KPNIVVAKDGSGQYKTIQEAIN 284
             + R LL       +D P WV    +R+L     K  KPN  VAKDGSG + TI +A+ 
Sbjct: 273 KVVERHLL-------DDIPSWVSNDDRRMLRAVDVKALKPNATVAKDGSGDFTTINDALR 332

Query: 285 KVPKRKNNATFVIHIKQGVYKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKT 344
            +P+ K    ++I++KQG+Y EYV + KK  +L ++GDG +KTI+TGNK+      TF T
Sbjct: 333 AMPE-KYEGRYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLT 392

Query: 345 ATVAVSAEHFMARDIGFENTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQF 404
           AT     E FMA+ +GF NTAGP  HQAVA+RVQ+D+ +F NC   GYQDTLY +T RQ+
Sbjct: 393 ATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQY 452

Query: 405 YRDCTISGTIDFVFGDAAAVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKF 464
           YR C I GTIDF+FGDAAA+FQNC+  +RK LP Q+N VTA GR ++ Q +  ++ N K 
Sbjct: 453 YRSCVIVGTIDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKI 512

Query: 465 KPDADLVPVKKQFRSYLGRPWKEYSRTIIMESYIDDIIDPEGWLPW-AGDWGLRTCFYTE 524
             + DL PVK++++SYLGRPWK YSRTIIMES I+++IDP GWL W   D+ + T +Y E
Sbjct: 513 AANEDLKPVKEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAE 572

Query: 525 FNNFGPGSGKAKRVKWRGIKSITPQHAVDFTPGRFILGDRWIKATGVPYVSGM 571
           +NN G       RVKW G K I  + A+++T G F+ GD WI A+G P   G+
Sbjct: 573 YNNKGSSGDTTSRVKWPGFKVINKEEALNYTVGPFLQGD-WISASGSPVKLGL 611

BLAST of CmoCh11G012460 vs. NCBI nr
Match: gi|449435986|ref|XP_004135775.1| (PREDICTED: putative pectinesterase/pectinesterase inhibitor 28 [Cucumis sativus])

HSP 1 Score: 983.0 bits (2540), Expect = 2.2e-283
Identity = 479/575 (83.30%), Postives = 534/575 (92.87%), Query Frame = 1

Query: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKA 60
           M Y D  ++N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQD TSDPATG K+HEISSSMKA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICRPTDYKRECVDSLKGSGK--SDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDP 120
           IKAIC+PTDYK+ECV SLK +G   SDPKELVQ GFKAAMKLIQAAA KSV L Q+EKDP
Sbjct: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120

Query: 121 RASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180
           RAS+ALA CKELMD+++DELK+S+ KLG FDISKLDEMLIDI+IWLSA ITYQETCLDGF
Sbjct: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ANTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRR-LLSIPVLGHEDF 240
           ANTTG+A+EKMKKALKTSM+LSSNGLAMV+++SSML+ELQIPGI+RR LL IPVLGH+D+
Sbjct: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300
           P+W +PGM+RLLA   +K KPN+VVAKDGSGQ+KTIQEAI++VPKRKNNAT+VIHIK GV
Sbjct: 241 PDWANPGMRRLLAAG-SKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGV 300

Query: 301 YKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFEN 360
           Y+EYV ++K LTHLMLIGDGPKKTIITGNKNF+DGT TFKTATVAV+AEHFMARDIGFEN
Sbjct: 301 YQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFEN 360

Query: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420
           TAGP KHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDCT+SGTIDF+FGDAAA
Sbjct: 361 TAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAA 420

Query: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480
           +FQ+C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQN  FKP ADLVPV+KQFRS+LGR
Sbjct: 421 IFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGR 480

Query: 481 PWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIK 540
           PWKEYSRTIIMESYI D+I PEGWLPWAGDWGLRTCFYTE+NN+GPGS K+KRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIK 540

Query: 541 SITPQHAVDFTPGRFILGDRWIKATGVPYVSGMTK 573
           +ITPQHAVDFTPGRF+ GDRWIK TGVPYVSG+T+
Sbjct: 541 NITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTR 574

BLAST of CmoCh11G012460 vs. NCBI nr
Match: gi|659099441|ref|XP_008450602.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo])

HSP 1 Score: 963.8 bits (2490), Expect = 1.4e-277
Identity = 466/575 (81.04%), Postives = 527/575 (91.65%), Query Frame = 1

Query: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKA 60
           M Y D  ++N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQD TSDPATG K+HEISSSMKA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICRPTDYKRECVDSLKGSGK--SDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDP 120
           IKAIC+PTDYK+EC+ SLK +G   SDP+ELVQ GFKAAMKLIQAAA KSV L+Q+EKDP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180
           R S+ALA CKELMD+++DELK+S+ KLG FDI+KLDEMLIDI+IWLSA ITYQETCLDGF
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ANTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRR-LLSIPVLGHEDF 240
            NTTG+A+EKMKKALKTSM+LSSNGLAMV++ SSMLTELQIPGI+RR LL IPVLGH+D+
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300
           P+W +PGM+RLLA   ++ KPN+VVAKDGSGQ++TIQ A+++VPKRKNNAT+VIHIK GV
Sbjct: 241 PDWANPGMRRLLAAG-SRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAGV 300

Query: 301 YKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFEN 360
           Y+EY+ I+K +THLMLIGDGPKKTIITGNKNFVDGT TFKTATVAV+AEHFMARDIGFEN
Sbjct: 301 YQEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFEN 360

Query: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420
           TAGP KHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDCT+SGTIDF+FGDAAA
Sbjct: 361 TAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAA 420

Query: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480
           +FQ C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQN   K D DL PV+KQFRS+LGR
Sbjct: 421 IFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLGR 480

Query: 481 PWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIK 540
           PWKEYSRTIIMESYI D+I PEGWLPWAGDWGL+TCFYTE+NN+GPGS K+KRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGIK 540

Query: 541 SITPQHAVDFTPGRFILGDRWIKATGVPYVSGMTK 573
           +ITPQHA+DFT GRF++GDRWIK TGVPYVSGMT+
Sbjct: 541 TITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTR 574

BLAST of CmoCh11G012460 vs. NCBI nr
Match: gi|703091827|ref|XP_010094457.1| (Putative pectinesterase/pectinesterase inhibitor 28 [Morus notabilis])

HSP 1 Score: 776.5 bits (2004), Expect = 3.1e-221
Identity = 382/581 (65.75%), Postives = 466/581 (80.21%), Query Frame = 1

Query: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKK--THEISSSM 60
           M+Y D   E RKRYA+IG+SS+LLVAMV+AVTVGV+LN +G S PA+  K  +HE+S+SM
Sbjct: 1   MSYGDDG-EKRKRYAVIGISSVLLVAMVIAVTVGVSLN-NGQSSPASSNKDGSHEVSASM 60

Query: 61  KAIKAICRPTDYKRECVDSL--KGSGKSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEK 120
           KAIK+IC+PTDYK EC++SL  +    +DPK+L+Q GFK AMK +  AAKKSV L+ +EK
Sbjct: 61  KAIKSICQPTDYKEECINSLSSESGNTTDPKKLIQAGFKVAMKYLSEAAKKSVVLQDLEK 120

Query: 121 DPRASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLD 180
           DPRAS+AL  CK+LM+ S+D+LK S+ KLG FDISK D+ML+D+KIWLSA +TYQETCLD
Sbjct: 121 DPRASKALGHCKQLMENSIDDLKHSVDKLGEFDISKFDQMLMDLKIWLSATVTYQETCLD 180

Query: 181 GFANTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRRLLS------IP 240
           GF NTTG+A+EKMKKALKTSMRLSSNGL MV+E+SS+ ++LQ+  + RRL S      +P
Sbjct: 181 GFQNTTGEAAEKMKKALKTSMRLSSNGLTMVSEISSVFSDLQLSSMGRRLFSDEEESELP 240

Query: 241 VLGHEDFPEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFV 300
           VLGH D  EW   G++RL+A  P K KPNIVVAKDGSG+Y TI EA+  +P+R  N TFV
Sbjct: 241 VLGHSDEAEWFGGGVRRLMAVHPTKVKPNIVVAKDGSGKYTTINEALKDIPRRSEN-TFV 300

Query: 301 IHIKQGVYKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMA 360
           I+IK+GVYKEYV I++ +T+L++IGDG  KT ITG+KNF+DG  T  TATV V  +HF A
Sbjct: 301 IYIKEGVYKEYVTIERNMTNLLIIGDGANKTRITGDKNFIDGFPTSLTATVVVLGDHFRA 360

Query: 361 RDIGFENTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDF 420
           RDIGFENTAG HKHQAVALRV AD+ +F+ C M GYQDTLY H  RQFYRDC +SGTIDF
Sbjct: 361 RDIGFENTAGAHKHQAVALRVNADEAIFYRCAMDGYQDTLYTHAKRQFYRDCNVSGTIDF 420

Query: 421 VFGDAAAVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQ 480
           VFGDAAAVFQNC F+VR+P+ NQQ IVTA GRKERRQPSA+IIQN  F  D D  PV+ +
Sbjct: 421 VFGDAAAVFQNCRFIVRRPMDNQQCIVTAQGRKERRQPSAIIIQNGTFVADPDYYPVRFE 480

Query: 481 FRSYLGRPWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGK-AK 540
             +YLGRPWKE+SRTIIMES+IDD+I P GWLPWAGD+GLRTCFYTEF N GPG+ + ++
Sbjct: 481 LPAYLGRPWKEFSRTIIMESFIDDLIQPRGWLPWAGDFGLRTCFYTEFGNRGPGAVEPSR 540

Query: 541 RVKWRGIKSITPQHAVDFTPGRFILGDRWIKATGVPYVSGM 571
           R KW+G+K+IT +HA DFTPG F  GD WIK TG+PYVSGM
Sbjct: 541 RAKWKGVKNITAKHARDFTPGSFFKGDWWIKRTGIPYVSGM 578

BLAST of CmoCh11G012460 vs. NCBI nr
Match: gi|802760279|ref|XP_012089439.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Jatropha curcas])

HSP 1 Score: 742.3 bits (1915), Expect = 6.6e-211
Identity = 369/582 (63.40%), Postives = 451/582 (77.49%), Query Frame = 1

Query: 4   DDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKK---THEISSSMKA 63
           DDA   N KR AIIG+SS+LLVAMVVAVT+GV  +++G S+  TGK    T EIS+SMKA
Sbjct: 3   DDA---NNKRLAIIGISSILLVAMVVAVTIGVGFSENGESEDITGKSHKSTGEISASMKA 62

Query: 64  IKAICRPTDYKRECVDSLKGSG--KSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDP 123
           IK+IC+P DYK+ C  SL+ S    +DPKEL++  FK A K I+ AAKKS TL ++EKDP
Sbjct: 63  IKSICQPADYKKTCEKSLEKSAGNTTDPKELIKAAFKIAQKKIREAAKKSATLEELEKDP 122

Query: 124 RASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 183
           R   AL  CKELMD S+ EL+ S  K+  FDI+ LD+ + D++ WLSAVITY+ TCLDGF
Sbjct: 123 RTRDALQSCKELMDMSISELQNSFDKVADFDITMLDDFMADLRTWLSAVITYEGTCLDGF 182

Query: 184 ANTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRRLLSIP-VLGHED- 243
            NTT DA EKMKKA+K ++ +S+NGL ++  LSS L +LQI G+NRRLL    VLGH+D 
Sbjct: 183 ENTTSDAGEKMKKAMKAAIEMSTNGLDIIDGLSSALADLQITGVNRRLLQEDEVLGHDDV 242

Query: 244 ---FPEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNAT--FVI 303
              FP WVD G +RLLA  P+K KPN+ VAKDGSG +KTI+ A++ +PK K  +T  FV+
Sbjct: 243 PSKFPSWVDAGTRRLLAAPPSKIKPNLTVAKDGSGDFKTIRGALDSIPKPKKGSTQTFVL 302

Query: 304 HIKQGVYKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMAR 363
           +IK GVY+EYV+  K + +LM+IGDGP+KT ITGNKNFVDG NT+ TATV V  ++F+A+
Sbjct: 303 YIKAGVYQEYVEFTKSMDYLMVIGDGPEKTRITGNKNFVDGVNTYHTATVVVLGDNFIAK 362

Query: 364 DIGFENTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFV 423
           +IGFEN+AG  KHQAVALRV AD  VF+NC M GYQDTLY H  RQFYR+C+ISGTIDFV
Sbjct: 363 NIGFENSAGAIKHQAVALRVSADFAVFYNCAMDGYQDTLYTHAKRQFYRECSISGTIDFV 422

Query: 424 FGDAAAVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQF 483
           FGDA  VFQNC FLVRKPL NQQ IVTA GRK+RRQPS +IIQN  F    +LVPVKKQ+
Sbjct: 423 FGDAPVVFQNCKFLVRKPLENQQCIVTAQGRKQRRQPSGIIIQNGTFTAHPELVPVKKQY 482

Query: 484 RSYLGRPWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRV 543
           +SYLGRPWK++SRTIIMESYIDDII+PEGWLPW G +GL+TC+YTEFNN+GPGS K  RV
Sbjct: 483 KSYLGRPWKQFSRTIIMESYIDDIIEPEGWLPWFGTFGLKTCWYTEFNNYGPGSDKKARV 542

Query: 544 KWRGIKSITPQHAVDFTPGRFILGDRWIKATGVPYVSGMTKP 574
           KW GIK+I  QHA+DFTPGRF+ GD WIK TG+PY   +T+P
Sbjct: 543 KWNGIKTIDRQHALDFTPGRFLRGDTWIKPTGIPYSPFLTRP 581

BLAST of CmoCh11G012460 vs. NCBI nr
Match: gi|255542792|ref|XP_002512459.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Ricinus communis])

HSP 1 Score: 737.3 bits (1902), Expect = 2.1e-209
Identity = 362/577 (62.74%), Postives = 454/577 (78.68%), Query Frame = 1

Query: 8   AENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTH---EISSSMKAIKAI 67
           ++ +KR AIIGVSS LLVAMVVAVTV +  N DG S+     K H   E+S+SMKAIK +
Sbjct: 7   SKRKKRLAIIGVSSFLLVAMVVAVTVSIGFNNDGESEDDINGKNHKSKEVSASMKAIKTL 66

Query: 68  CRPTDYKRECVDSL-KGSGKS-DPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDPRASQ 127
           C+PT YK+ C  SL K +G + DPKEL+++ FK A K I +A+KKS+TL ++EKDPR   
Sbjct: 67  CQPTYYKQTCERSLAKSAGNTTDPKELIKIAFKLAEKQIDSASKKSLTLLELEKDPRTRG 126

Query: 128 ALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANTT 187
           AL  CKELM  S++EL+ S++K+  FD S+LDE++ DIK WLSA ITY+ETCLD F NTT
Sbjct: 127 ALNSCKELMTMSINELRSSLEKVADFDFSQLDELMADIKTWLSAAITYEETCLDAFENTT 186

Query: 188 GDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRRLLS--IPVLGHED---- 247
            +A EKMKKALKT+M +SSNGL +V+ +SS+LT+LQIPG++RRLL   IPV GH D    
Sbjct: 187 TNAGEKMKKALKTAMEMSSNGLDIVSGISSVLTDLQIPGVSRRLLQDDIPVAGHGDISQA 246

Query: 248 FPEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQG 307
           FP W+DPG +RLL+  P+  KP++VVAKDGSG YKTI EA+ ++PK K+N TFV++IK+G
Sbjct: 247 FPAWIDPGTRRLLSAPPSNIKPDLVVAKDGSGDYKTILEALPQIPK-KSNETFVLYIKEG 306

Query: 308 VYKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFE 367
           +Y+EYV+  + +T+L++IGDGP KT ITG+KNFVDG NT++TATVAV  ++F+AR+IGFE
Sbjct: 307 IYEEYVEFNRSMTNLVVIGDGPDKTRITGSKNFVDGINTYRTATVAVIGDNFVARNIGFE 366

Query: 368 NTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAA 427
           N+AG  KHQAVALRV +D  VF+NC M GYQDTLY H  RQFYRDCT+SGTIDFVFGDA 
Sbjct: 367 NSAGAIKHQAVALRVSSDYAVFYNCSMDGYQDTLYTHAKRQFYRDCTVSGTIDFVFGDAP 426

Query: 428 AVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLG 487
            VFQNC+FLVRKPL NQQ IVTA GRK RRQPSA+IIQNS      +L PVK Q++SYLG
Sbjct: 427 VVFQNCTFLVRKPLENQQCIVTAQGRKARRQPSAIIIQNSTITAHPELEPVKDQYKSYLG 486

Query: 488 RPWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGI 547
           RPWKE+SRTIIME++IDD+I PEGW PW G +GL+TC+Y E+NN+GPGS    RVKW GI
Sbjct: 487 RPWKEFSRTIIMETFIDDLIQPEGWSPWFGSFGLKTCWYGEYNNYGPGSDMKNRVKWNGI 546

Query: 548 KSITPQHAVDFTPGRFILGDRWIKATGVPYVSGMTKP 574
           K ++ QHA+DFTPGRF+ GD WIK TGVPY   +T+P
Sbjct: 547 KPVSRQHAIDFTPGRFLRGDSWIKPTGVPYAPYLTRP 582

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME28_ARATH1.9e-18655.59Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana GN=P... [more]
PME21_ARATH9.9e-18355.23Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana GN=P... [more]
PME58_ARATH3.2e-14947.28Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana GN=P... [more]
PME23_ARATH6.5e-14245.36Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana GN=P... [more]
PME_MEDSA2.2e-12650.22Pectinesterase/pectinesterase inhibitor OS=Medicago sativa PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LYF5_CUCSA1.5e-28383.30Pectinesterase OS=Cucumis sativus GN=Csa_1G569220 PE=4 SV=1[more]
W9S2R1_9ROSA2.2e-22165.75Pectinesterase OS=Morus notabilis GN=L484_001919 PE=4 SV=1[more]
A0A067JV77_JATCU4.6e-21163.40Pectinesterase OS=Jatropha curcas GN=JCGZ_23282 PE=4 SV=1[more]
B9RFE0_RICCO1.5e-20962.74Pectinesterase OS=Ricinus communis GN=RCOM_1433930 PE=4 SV=1[more]
D7U9X3_VITVI1.1e-20162.26Pectinesterase OS=Vitis vinifera GN=VIT_14s0060g01950 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G27870.11.1e-18755.59 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G05610.15.6e-18455.23 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G49180.11.8e-15047.28 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G06830.13.6e-14345.36 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT2G26450.11.3e-11941.09 Plant invertase/pectin methylesterase inhibitor superfamily[more]
Match NameE-valueIdentityDescription
gi|449435986|ref|XP_004135775.1|2.2e-28383.30PREDICTED: putative pectinesterase/pectinesterase inhibitor 28 [Cucumis sativus][more]
gi|659099441|ref|XP_008450602.1|1.4e-27781.04PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo][more]
gi|703091827|ref|XP_010094457.1|3.1e-22165.75Putative pectinesterase/pectinesterase inhibitor 28 [Morus notabilis][more]
gi|802760279|ref|XP_012089439.1|6.6e-21163.40PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Jatropha curcas][more]
gi|255542792|ref|XP_002512459.1|2.1e-20962.74PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Ricinus communis... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000070Pectinesterase_cat
IPR006501Pectinesterase_inhib_dom
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
IPR018040Pectinesterase_Tyr_AS
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
GO:0004857enzyme inhibitor activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005618 cell wall
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh11G012460.1CmoCh11G012460.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 259..556
score: 2.4E
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 58..211
score: 1.7
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 60..206
score: 2.2
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 56..206
score: 6.6
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 57..210
score: 2.9
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 59..211
score: 1.05
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 257..569
score: 4.71E
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 256..569
score: 7.5E
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 285..304
scor
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 11..572
score:
NoneNo IPR availablePANTHERPTHR31707:SF17SUBFAMILY NOT NAMEDcoord: 11..572
score: