Cucsa.178510 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.178510 vs. Swiss-Prot
Match: CPSF1_ARATH (Cleavage and polyadenylation specificity factor subunit 1 OS=Arabidopsis thaliana GN=CPSF160 PE=1 SV=2)

HSP 1 Score: 2099.7 bits (5439), Expect = 0.0e+00
Identity = 1042/1458 (71.47%), Postives = 1224/1458 (83.95%), Query Frame = 1

Query: 1    MSFAAYRMMHWPTGIENCDSAYITHSRADF---VPAVTSHSDDLDSDW-HPRRDIGPVPN 60
            MSFAAY+MMHWPTG+ENC S YITHS +D    +P V+ H DD++++W +P+R IGP+PN
Sbjct: 1    MSFAAYKMMHWPTGVENCASGYITHSLSDSTLQIPIVSVH-DDIEAEWPNPKRGIGPLPN 60

Query: 61   LVVTAGNVLEVYVVRVLEEGG-RESKSSGEVRRGGIMDGVSGASLELVCHYRLHGNVESM 120
            +V+TA N+LEVY+VR  EEG  +E ++    +RGG+MDGV G SLELVCHYRLHGNVES+
Sbjct: 61   VVITAANILEVYIVRAQEEGNTQELRNPKLAKRGGVMDGVYGVSLELVCHYRLHGNVESI 120

Query: 121  AILSSRGGDGSKKRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWLHLKRGRES 180
            A+L   GG+ SK RDSIIL F++AKISVLEFDDS HSLR +SMHCF+GP WLHLKRGRES
Sbjct: 121  AVLPMGGGNSSKGRDSIILTFRDAKISVLEFDDSIHSLRMTSMHCFEGPDWLHLKRGRES 180

Query: 181  FARGPVVKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLI 240
            F RGP+VKVDPQGRCGGVLVYGLQMIILK SQ GSGLV DD+AF + G +SARVESSY+I
Sbjct: 181  FPRGPLVKVDPQGRCGGVLVYGLQMIILKTSQVGSGLVGDDDAFSSGGTVSARVESSYII 240

Query: 241  NLRDLDVKHVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALSISTTLKQHP 300
            NLRDL++KHVKDFVF+HGYIEPV+VIL E+E TWAGRVSWKHHTC++SALSI++TLKQHP
Sbjct: 241  NLRDLEMKHVKDFVFLHGYIEPVIVILQEEEHTWAGRVSWKHHTCVLSALSINSTLKQHP 300

Query: 301  LIWSASNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCMLALNNYAVSADSSQDMP 360
            +IWSA NLPHDAYKLLAVPSPIGGVLV+ AN+IHY+SQSASC LALNNYA SADSSQ++P
Sbjct: 301  VIWSAINLPHDAYKLLAVPSPIGGVLVLCANTIHYHSQSASCALALNNYASSADSSQELP 360

Query: 361  RSNFNVELDAANATWLVNDVALLSTKTGELLLLALVYDGRVVQRLDLSKSKASVLTSGIA 420
             SNF+VELDAA+ TW+ NDVALLSTK+GELLLL L+YDGR VQRLDLSKSKASVL S I 
Sbjct: 361  ASNFSVELDAAHGTWISNDVALLSTKSGELLLLTLIYDGRAVQRLDLSKSKASVLASDIT 420

Query: 421  SIGNSLFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMRRSSSDALQ 480
            S+GNSLFFLGSRLGDSLLVQFSC  G +     L+DE  DIE + H AKR+ R +SD  Q
Sbjct: 421  SVGNSLFFLGSRLGDSLLVQFSCRSGPAASLPGLRDEDEDIEGEGHQAKRL-RMTSDTFQ 480

Query: 481  DMVGGDELSLYGSAANNTESAQKIFSFAVRDSLINIGPLKDFSYGLRINADPNATGIAKQ 540
            D +G +ELSL+GS  NN++SAQK FSFAVRDSL+N+GP+KDF+YGLRINAD NATG++KQ
Sbjct: 481  DTIGNEELSLFGSTPNNSDSAQKSFSFAVRDSLVNVGPVKDFAYGLRINADANATGVSKQ 540

Query: 541  SNYELVCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVYHKNTRGSIADSSRTVP 600
            SNYELVCCSGHGKNGALC+LRQSIRPEMITEVELPGCKGIWTVYHK++RG  ADSS+   
Sbjct: 541  SNYELVCCSGHGKNGALCVLRQSIRPEMITEVELPGCKGIWTVYHKSSRGHNADSSKMAA 600

Query: 601  DDDEYHAYLIISLEARTMVLVTGELLTEVTESVDYFVHGRTIAAGNLFGRRRVIQVYESG 660
            D+DEYHAYLIISLEARTMVL T +LLTEVTESVDY+V GRTIAAGNLFGRRRVIQV+E G
Sbjct: 601  DEDEYHAYLIISLEARTMVLETADLLTEVTESVDYYVQGRTIAAGNLFGRRRVIQVFEHG 660

Query: 661  ARILDGSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMTDGSIRLLVGDSSSC 720
            ARILDGSFM Q+L+   + +ES + SE  TV S SI+DPYVLL MTD SIRLLVGD S+C
Sbjct: 661  ARILDGSFMNQELSFGASNSESNSGSESSTVSSVSIADPYVLLRMTDDSIRLLVGDPSTC 720

Query: 721  SVSVSAPAAFGSSKKCVSSCTLYQDKGFEPWLRMTSTDAWLSTGVGETIDGTDGSLQDQG 780
            +VS+S+P+    SK+ +S+CTLY DKG EPWLR  STDAWLS+GVGE +D  DG  QDQG
Sbjct: 721  TVSISSPSVLEGSKRKISACTLYHDKGPEPWLRKASTDAWLSSGVGEAVDSVDGGPQDQG 780

Query: 781  DIYCVACYDNGDLEIFDVPNFTSVFYVDKFVSGKSHLVDHQISDLQKSSEVDQNSQELIS 840
            DIYCV CY++G LEIFDVP+F  VF VDKF SG+ HL D  I +L+   E+++NS++  S
Sbjct: 781  DIYCVVCYESGALEIFDVPSFNCVFSVDKFASGRRHLSDMPIHELE--YELNKNSEDNTS 840

Query: 841  HGRNESSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFESTDSASKIDDSVS 900
               ++  +N +V+E+AMQRWSG H+RPFLF +L DGTILCYHAYLF+  DS +K ++S+S
Sbjct: 841  ---SKEIKNTRVVELAMQRWSGHHTRPFLFAVLADGTILCYHAYLFDGVDS-TKAENSLS 900

Query: 901  IDNSVSSSNMSSSRLRNLRFLRVPLDIQGREDMPNGTLSRRLSIFKNISGYQGLFLCGSR 960
             +N  + ++  SS+LRNL+FLR+PLD   RE   +G  S+R+++FKNISG+QG FL GSR
Sbjct: 901  SENPAALNSSGSSKLRNLKFLRIPLDTSTREGTSDGVASQRITMFKNISGHQGFFLSGSR 960

Query: 961  PAWFMVFRERLRVHPQLCDGPIVAFAVLHNVNCNHGLIYVTSQ----------------- 1020
            P W M+FRERLR H QLCDG I AF VLHNVNCNHG IYVT+Q                 
Sbjct: 961  PGWCMLFRERLRFHSQLCDGSIAAFTVLHNVNCNHGFIYVTAQGVLKICQLPSASIYDNY 1020

Query: 1021 -----VPLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQVLSSMVDQDVG-HVENHNLSAD 1080
                 +PLK TPHQVTY+ EKNLYP+I+S PV KPLNQVLSS+VDQ+ G  ++NHN+S+D
Sbjct: 1021 WPVQKIPLKATPHQVTYYAEKNLYPLIVSYPVSKPLNQVLSSLVDQEAGQQLDNHNMSSD 1080

Query: 1081 ELQQTYSVEEFEIRILEPEKSGGPWQTRATIAMHSSENALTIRVVTLLNTTTKENETLLA 1140
            +LQ+TY+VEEFEI+ILEPE+SGGPW+T+A I M +SE+ALT+RVVTLLN +T ENETLLA
Sbjct: 1081 DLQRTYTVEEFEIQILEPERSGGPWETKAKIPMQTSEHALTVRVVTLLNASTGENETLLA 1140

Query: 1141 VGTAYVQGEDVAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALASLQGHLLIASG 1200
            VGTAYVQGEDVAARGRVLLFS GK+ DNSQ +V+EVYS+ELKGAISA+AS+QGHLLI+SG
Sbjct: 1141 VGTAYVQGEDVAARGRVLLFSFGKNGDNSQNVVTEVYSRELKGAISAVASIQGHLLISSG 1200

Query: 1201 PKIILHKWTGAELNGIAFYDVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLL 1260
            PKIILHKW G ELNG+AF+D PPLYVVS+N+VK+FILLGD+HKSIYFLSWKEQG+QLSLL
Sbjct: 1201 PKIILHKWNGTELNGVAFFDAPPLYVVSMNVVKSFILLGDVHKSIYFLSWKEQGSQLSLL 1260

Query: 1261 AKDFGSLDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTESWKGQKLLSRAEFHVGA 1320
            AKDF SLDC+ATEFLIDGSTLSL VSD+QKNIQ+FYYAPK  ESWKG KLLSRAEFHVGA
Sbjct: 1261 AKDFESLDCFATEFLIDGSTLSLAVSDEQKNIQVFYYAPKMIESWKGLKLLSRAEFHVGA 1320

Query: 1321 HVTKFLRLQMLSTSSDKACSTVSDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKK 1380
            HV+KFLRLQM+S+         +DK NRFALLFGTLDGS GCIAPLDE+TFRRLQSLQKK
Sbjct: 1321 HVSKFLRLQMVSSG--------ADKINRFALLFGTLDGSFGCIAPLDEVTFRRLQSLQKK 1380

Query: 1381 LGDAVPHVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELLCHYEMLPLEEQLDIAHQIGTT 1431
            L DAVPHV GLNP +FRQF S+GK  R GPDSIVDCELLCHYEMLPLEEQL++AHQIGTT
Sbjct: 1381 LVDAVPHVAGLNPLAFRQFRSSGKARRSGPDSIVDCELLCHYEMLPLEEQLELAHQIGTT 1440

BLAST of Cucsa.178510 vs. Swiss-Prot
Match: CPSF1_ORYSJ (Probable cleavage and polyadenylation specificity factor subunit 1 OS=Oryza sativa subsp. japonica GN=Os04g0252200 PE=3 SV=2)

HSP 1 Score: 1825.4 bits (4727), Expect = 0.0e+00
Identity = 951/1468 (64.78%), Postives = 1131/1468 (77.04%), Query Frame = 1

Query: 1    MSFAAYRMMHWPTGIENCDSAYITHSRAD---FVPAVT---SHSDDLDSDW---HPRRDI 60
            MS+AAY+MMHWPTG+++C + ++THS +D   F  A T       D+DS      PRR +
Sbjct: 1    MSYAAYKMMHWPTGVDHCAAGFVTHSPSDAAAFFTAATVGPGPEGDIDSAAAASRPRR-L 60

Query: 61   GPVPNLVVTAGNVLEVYVVRV---LEEGGRESKSSGEVRRGGIMDGVSGASLELVCHYRL 120
            GP PNLVV A NVLEVY VR     E+GG  ++ S     G ++DG+SGA LELVC+YRL
Sbjct: 61   GPSPNLVVAAANVLEVYAVRAETAAEDGGGGTQPSSS--SGAVLDGISGARLELVCYYRL 120

Query: 121  HGNVESMAILSSRGGDGSK-KRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWL 180
            HGN+ESM +LS    DG++ +R +I L F++AKI+ LEFDD+ H LRTSSMHCF+GP+W 
Sbjct: 121  HGNIESMTVLS----DGAENRRATIALAFKDAKITCLEFDDAIHGLRTSSMHCFEGPEWQ 180

Query: 181  HLKRGRESFARGPVVKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISA 240
            HLKRGRESFA GPV+K DP GRCG  L YGLQMIILKA+Q G  LV +DE      + + 
Sbjct: 181  HLKRGRESFAWGPVIKADPLGRCGAALAYGLQMIILKAAQVGHSLVGEDEPTCALSSTAV 240

Query: 241  RVESSYLINLRDLDVKHVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALSI 300
             +ESSYLI+LR LD+ HVKDF FVHGYIEPV+VILHEQE TWAGR+  KHHTCM+SA SI
Sbjct: 241  CIESSYLIDLRALDMNHVKDFAFVHGYIEPVLVILHEQEPTWAGRILSKHHTCMISAFSI 300

Query: 301  STTLKQHPLIWSASNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCMLALNNYAVS 360
            S TLKQHP+IWSA+NLPHDAY+LLAVP PI GVLVI ANSIHY+SQS SC L LNN++  
Sbjct: 301  SMTLKQHPVIWSAANLPHDAYQLLAVPPPISGVLVICANSIHYHSQSTSCSLDLNNFSSH 360

Query: 361  ADSSQDMPRSNFNVELDAANATWLVNDVALLSTKTGELLLLALVYDGRVVQRLDLSKSKA 420
             D S ++ +SNF VELDAA ATWL ND+ + STK GE+LLL +VYDGRVVQRLDL KSKA
Sbjct: 361  PDGSPEISKSNFQVELDAAKATWLSNDIVMFSTKAGEMLLLTVVYDGRVVQRLDLMKSKA 420

Query: 421  SVLTSGIASIGNSLFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMR 480
            SVL+S + SIGNS FFLGSRLGDSLLVQFS     S L     +   DIE D   +KR++
Sbjct: 421  SVLSSAVTSIGNSFFFLGSRLGDSLLVQFSYCASKSVLQDLTNERSADIEGDLPFSKRLK 480

Query: 481  RSSSDALQDMVGGDELSLYG-SAANNTESAQKIFSFAVRDSLINIGPLKDFSYGLRINAD 540
            R  SD LQD+   +ELS     A N+ ESAQKI S+ VRD+LIN+GPLKDFSYGLR NAD
Sbjct: 481  RIPSDVLQDVTSVEELSFQNIIAPNSLESAQKI-SYIVRDALINVGPLKDFSYGLRANAD 540

Query: 541  PNATGIAKQSNYELVCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVYHKNTRGS 600
            PNA G AKQSNYELVCCSGHGKNG+L +L+QSIRP++ITEVELP C+GIWTVY+K+ RG 
Sbjct: 541  PNAMGNAKQSNYELVCCSGHGKNGSLSVLQQSIRPDLITEVELPSCRGIWTVYYKSYRGQ 600

Query: 601  IADSSRTVPDDDEYHAYLIISLEARTMVLVTGELLTEVTESVDYFVHGRTIAAGNLFGRR 660
            +A       +D+EYHAYLIISLE RTMVL TG+ L EVTE+VDYFV   TIAAGNLFGRR
Sbjct: 601  MA-------EDNEYHAYLIISLENRTMVLETGDDLGEVTETVDYFVQASTIAAGNLFGRR 660

Query: 661  RVIQVYESGARILDGSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMTDGSIR 720
            RVIQVY  GAR+LDGSFMTQ+LN   + +ES ++SE   V  ASI+DPYVLL M DGS++
Sbjct: 661  RVIQVYGKGARVLDGSFMTQELNFTTHASES-SSSEALGVACASIADPYVLLKMVDGSVQ 720

Query: 721  LLVGDSSSCSVSVSAPAAFGSSKKCVSSCTLYQDKGFEPWLRMTSTDAWLSTGVGETIDG 780
            LL+GD  +C++SV+AP+ F SS + +++CTLY+D+G EPWL  T +DAWLSTG+ E IDG
Sbjct: 721  LLIGDYCTCTLSVNAPSIFISSSERIAACTLYRDRGPEPWLTKTRSDAWLSTGIAEAIDG 780

Query: 781  TDGSLQDQGDIYCVACYDNGDLEIFDVPNFTSVFYVDKFVSGKSHLVDHQISDLQKSSEV 840
               S  DQ DIYC+ CY++G LEIF+VP+F  VF V+ F+SG++ LVD + S L      
Sbjct: 781  NGTSSHDQSDIYCIICYESGKLEIFEVPSFRCVFSVENFISGEALLVD-KFSQLIYEDST 840

Query: 841  DQNSQELISHGRNESSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFESTDS 900
             +      +  + E+  +++++E+AM RWSGQ SRPFLFG+L DGT+LCYHA+ +E+++S
Sbjct: 841  KERYDCTKASLKKEAGDSIRIVELAMHRWSGQFSRPFLFGLLNDGTLLCYHAFSYEASES 900

Query: 901  ASKIDDSVSIDNSVSSSNMSSSRLRNLRFLRVPLDIQGREDMPNGTLSR-RLSIFKNISG 960
              K    +S   S    N S SRLRNLRF RV +DI  RED+P  TL R R++ F N+ G
Sbjct: 901  NVK-RVPLSPQGSADHHNASDSRLRNLRFHRVSIDITSREDIP--TLGRPRITTFNNVGG 960

Query: 961  YQGLFLCGSRPAWFMVFRERLRVHPQLCDGPIVAFAVLHNVNCNHGLIYVTSQ------- 1020
            Y+GLFL G+RPAW MV R+RLRVHPQLCDGPI AF VLHNVNC+HG IYVTSQ       
Sbjct: 961  YEGLFLSGTRPAWVMVCRQRLRVHPQLCDGPIEAFTVLHNVNCSHGFIYVTSQGFLKICQ 1020

Query: 1021 ---------------VPLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQVLSSMVDQD-VG 1080
                           VPL GTPHQVTY+ E++LYP+I+S PV +PLNQVLSSM DQ+ V 
Sbjct: 1021 LPSAYNYDSYWPVQKVPLHGTPHQVTYYAEQSLYPLIVSVPVVRPLNQVLSSMADQESVH 1080

Query: 1081 HVENHNLSADELQQTYSVEEFEIRILEPEKSGGPWQTRATIAMHSSENALTIRVVTLLNT 1140
            H++N   S D L +TY+V+EFE+RILE EK GG W+T++TI M   ENALT+R+VTL NT
Sbjct: 1081 HMDNDVTSTDALHKTYTVDEFEVRILELEKPGGHWETKSTIPMQLFENALTVRIVTLHNT 1140

Query: 1141 TTKENETLLAVGTAYVQGEDVAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALAS 1200
            TTKENETLLA+GTAYV GEDVAARGRVLLFS  K ++NSQ LV+EVYSKE KGA+SA+AS
Sbjct: 1141 TTKENETLLAIGTAYVLGEDVAARGRVLLFSFTK-SENSQNLVTEVYSKESKGAVSAVAS 1200

Query: 1201 LQGHLLIASGPKIILHKWTGAELNGIAFYDVPPLYVVSLNIVKNFILLGDIHKSIYFLSW 1260
            LQGHLLIASGPKI L+KWTGAEL  +AFYD  PL+VVSLNIVKNF+L GDIHKSIYFLSW
Sbjct: 1201 LQGHLLIASGPKITLNKWTGAELTAVAFYDA-PLHVVSLNIVKNFVLFGDIHKSIYFLSW 1260

Query: 1261 KEQGAQLSLLAKDFGSLDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTESWKGQKL 1320
            KEQG+QLSLLAKDFGSLDC+ATEFLIDGSTLSL  SD  KN+QIFYYAPK  ESWKGQKL
Sbjct: 1261 KEQGSQLSLLAKDFGSLDCFATEFLIDGSTLSLVASDSDKNVQIFYYAPKMVESWKGQKL 1320

Query: 1321 LSRAEFHVGAHVTKFLRLQMLSTSSDKACSTVSDKTNRFALLFGTLDGSIGCIAPLDELT 1380
            LSRAEFHVGAH+TKFLRLQML T         S+KTNRFALLFG LDG IGCIAP+DELT
Sbjct: 1321 LSRAEFHVGAHITKFLRLQMLPTQ-----GLSSEKTNRFALLFGNLDGGIGCIAPIDELT 1380

Query: 1381 FRRLQSLQKKLGDAVPHVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELLCHYEMLPLEEQ 1431
            FRRLQSLQ+KL DAVPHV GLNPRSFRQFHSNGK HR GPD+I+D ELLC YEML L+EQ
Sbjct: 1381 FRRLQSLQRKLVDAVPHVCGLNPRSFRQFHSNGKGHRPGPDNIIDFELLCSYEMLSLDEQ 1440

BLAST of Cucsa.178510 vs. Swiss-Prot
Match: CPSF1_HUMAN (Cleavage and polyadenylation specificity factor subunit 1 OS=Homo sapiens GN=CPSF1 PE=1 SV=2)

HSP 1 Score: 342.4 bits (877), Expect = 2.3e-92
Identity = 236/681 (34.65%), Postives = 362/681 (53.16%), Query Frame = 1

Query: 56  NLVVTAGNVLEVYVVRVLEEGGRESKSSGEVRRGGIMDGVSGASLELVCHYRLHGNVESM 115
           NLVV   + L VY +    E   ++  S E +            LEL   +   GNV SM
Sbjct: 29  NLVVAGTSQLYVYRLNRDAEALTKNDRSTEGK-------AHREKLELAASFSFFGNVMSM 88

Query: 116 AILSSRGGDGSKKRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWLHLKRGRES 175
           A +   G     KRD+++L F++AK+SV+E+D  TH L+T S+H F+ P+   L+ G   
Sbjct: 89  ASVQLAGA----KRDALLLSFKDAKLSVVEYDPGTHDLKTLSLHYFEEPE---LRDGFVQ 148

Query: 176 FARGPVVKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLI 235
               P V+VDP GRC  +LVYG ++++L   +    L  + E     G  S+ + S Y+I
Sbjct: 149 NVHTPRVRVDPDGRCAAMLVYGTRLVVLPFRR--ESLAEEHEGLVGEGQRSSFLPS-YII 208

Query: 236 NLRDLDVK--HVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALSISTTLKQ 295
           ++R LD K  ++ D  F+HGY EP ++IL E   TW GRV+ +  TC + A+S++ T K 
Sbjct: 209 DVRALDEKLLNIIDLQFLHGYYEPTLLILFEPNQTWPGRVAVRQDTCSIVAISLNITQKV 268

Query: 296 HPLIWSASNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCM-LALNNYAVSADSSQ 355
           HP+IWS ++LP D  + LAVP PIGGV+V + NS+ Y +QS     +ALN+      +  
Sbjct: 269 HPVIWSLTSLPFDCTQALAVPKPIGGVVVFAVNSLLYLNQSVPPYGVALNSLTTGTTAFP 328

Query: 356 DMPRSNFNVELDAANATWLVNDVALLSTKTGELLLLALVYDG-RVVQRLDLSKSKASVLT 415
              +    + LD A AT++  D  ++S K GE+ +L L+ DG R V+     K+ ASVLT
Sbjct: 329 LRTQEGVRITLDCAQATFISYDKMVISLKGGEIYVLTLITDGMRSVRAFHFDKAAASVLT 388

Query: 416 SGIASIGNSLFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRM----- 475
           + + ++     FLGSRLG+SLL++++  +      ++   E  D E      KR+     
Sbjct: 389 TSMVTMEPGYLFLGSRLGNSLLLKYTEKLQEP--PASAVREAADKEEPPSKKKRVDATAG 448

Query: 476 -RRSSSDALQDMVGGDELSLYGS-AANNTESAQKIFSFAVRDSLINIGPLKDFSYGLRIN 535
              +     QD V  DE+ +YGS A + T+ A   +SF V DS++NIGP  + + G    
Sbjct: 449 WSAAGKSVPQDEV--DEIEVYGSEAQSGTQLA--TYSFEVCDSILNIGPCANAAVGEPAF 508

Query: 536 ADPNATGIAKQSNYELVCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVY----- 595
                   + + + E+V CSGHGKNGAL +L++SIRP+++T  ELPGC  +WTV      
Sbjct: 509 LSEEFQN-SPEPDLEIVVCSGHGKNGALSVLQKSIRPQVVTTFELPGCYDMWTVIAPVRK 568

Query: 596 --HKNTRGSIADSS-RTVP---DDDEYHAYLIISLEARTMVLVTGELLTEVTESVDYFVH 655
               N +G   +    T P   DD   H +LI+S E  TM+L TG+ + E+  S  +   
Sbjct: 569 EEEDNPKGEGTEQEPSTTPEADDDGRRHGFLILSREDSTMILQTGQEIMELDTS-GFATQ 628

Query: 656 GRTIAAGNLFGRRRVIQVYESGARILDGSFMTQDLNLVVNGNESGNASEGCTVLSASISD 715
           G T+ AGN+   R ++QV   G R+L+G          VN         G  ++  +++D
Sbjct: 629 GPTVFAGNIGDNRYIVQVSPLGIRLLEG----------VNQLHFIPVDLGAPIVQCAVAD 674

HSP 2 Score: 292.7 bits (748), Expect = 2.1e-77
Identity = 215/689 (31.20%), Postives = 334/689 (48.48%), Query Frame = 1

Query: 778  YCVACYDNGDLEIFDVPNFTSVFYVDKFVSGKSHLVDHQISDLQKSSEVDQNSQELISHG 837
            +C+   +NG +EI+ +P++  VF V  F  G+  LVD      Q +++ +   +E    G
Sbjct: 784  WCLLVRENGTMEIYQLPDWRLVFLVKNFPVGQRVLVDSSFG--QPTTQGEARREEATRQG 843

Query: 838  RNESSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFESTDSASKIDDSVSID 897
                 +   V EV +     + SRP+L  +  D  +L Y A+                 D
Sbjct: 844  -----ELPLVKEVLLVALGSRQSRPYLL-VHVDQELLIYEAFPH---------------D 903

Query: 898  NSVSSSNMSSSRLRNLRFLRVPLDIQGREDMPNGTLSR-----------------RLSIF 957
            + +   N+       +RF +VP +I  RE  P  +  +                 R   F
Sbjct: 904  SQLGQGNLK------VRFKKVPHNINFREKKPKPSKKKAEGGGAEEGAGARGRVARFRYF 963

Query: 958  KNISGYQGLFLCGSRPAWFMVF-RERLRVHPQLCDGPIVAFAVLHNVNCNHGLIYVTSQV 1017
            ++I GY G+F+CG  P W +V  R  LR+HP   DGP+ +FA  HNVNC  G +Y   Q 
Sbjct: 964  EDIYGYSGVFICGPSPHWLLVTGRGALRLHPMAIDGPVDSFAPFHNVNCPRGFLYFNRQG 1023

Query: 1018 PLKGT--PHQVTYFHEKNLYPV-------IISAPVQKPLNQVLSS------MVDQDVGHV 1077
             L+ +  P  ++Y     +  +        ++  V+  +  V +S       + +  G  
Sbjct: 1024 ELRISVLPAYLSYDAPWPVRKIPLRCTAHYVAYHVESKVYAVATSTNTPCARIPRMTGEE 1083

Query: 1078 -ENHNLSADELQQTYSVEEFEIRILEPEKSGGPWQT--RATIAMHSSENALTIRVVTLLN 1137
             E   +  DE       E F I+++ P      W+    A I +   E+   ++ V+L +
Sbjct: 1084 KEFETIERDERYIHPQQEAFSIQLISPVS----WEAIPNARIELQEWEHVTCMKTVSLRS 1143

Query: 1138 TTTKEN-ETLLAVGTAYVQGEDVAARGRVLLFSVGKDA-DNSQTLVSE----VYSKELKG 1197
              T    +  +A GT  +QGE+V  RGR+L+  V +   +  Q L       +Y KE KG
Sbjct: 1144 EETVSGLKGYVAAGTCLMQGEEVTCRGRILIMDVIEVVPEPGQPLTKNKFKVLYEKEQKG 1203

Query: 1198 AISALASLQGHLLIASGPKIILHKWTGAELNGIAFYDVPPLYVVSLNIVKNFILLGDIHK 1257
             ++AL    GHL+ A G KI L     +EL G+AF D   LY+  +  VKNFIL  D+ K
Sbjct: 1204 PVTALCHCNGHLVSAIGQKIFLWSLRASELTGMAFIDT-QLYIHQMISVKNFILAADVMK 1263

Query: 1258 SIYFLSWKEQGAQLSLLAKDFGSLDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTE 1317
            SI  L ++E+   LSL+++D   L+ Y+ +F++D + L   VSD  +N+ ++ Y P++ E
Sbjct: 1264 SISLLRYQEESKTLSLVSRDAKPLEVYSVDFMVDNAQLGFLVSDRDRNLMVYMYLPEAKE 1323

Query: 1318 SWKGQKLLSRAEFHVGAHVTKFLRLQMLSTSSDKACSTVSDKT----NRFALLFGTLDGS 1377
            S+ G +LL RA+FHVGAHV  F R     T    A   +S K+    N+    F TLDG 
Sbjct: 1324 SFGGMRLLRRADFHVGAHVNTFWR-----TPCRGATEGLSKKSVVWENKHITWFATLDGG 1383

Query: 1378 IGCIAPLDELTFRRLQSLQKKLGDAVPHVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELL 1421
            IG + P+ E T+RRL  LQ  L   +PH  GLNPR+FR  H + +  +    +++D ELL
Sbjct: 1384 IGLLLPMQEKTYRRLLMLQNALTTMLPHHAGLNPRAFRMLHVDRRTLQNAVRNVLDGELL 1433

BLAST of Cucsa.178510 vs. Swiss-Prot
Match: CPSF1_MOUSE (Cleavage and polyadenylation specificity factor subunit 1 OS=Mus musculus GN=Cpsf1 PE=1 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 5.2e-92
Identity = 252/794 (31.74%), Postives = 402/794 (50.63%), Query Frame = 1

Query: 3   FAAYRMMHWPTGIE-NCDSAYITHSRADFVPAVTSHSDDLDSDWHPRRDIGPVPNLVVTA 62
           +A Y+  H PTG+E      +  +S  + V A TS                         
Sbjct: 2   YAVYKQAHPPTGLEFTMYCNFFNNSERNLVVAGTS------------------------- 61

Query: 63  GNVLEVYVVRVLEEGGRESKSSGEVRRGGIMDGVSGASLELVCHYRLHGNVESMAILSSR 122
               ++YV R+  +    +K+ G        +      LELV  +   GNV SMA +   
Sbjct: 62  ----QLYVYRLNRDAEALTKNDGSTEGKAHRE-----KLELVASFSFFGNVMSMASVQLA 121

Query: 123 GGDGSKKRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWLHLKRGRESFARGPV 182
           G     KRD+++L F++AK+SV+E+D  TH L+T S+H F+ P+   L+ G       P 
Sbjct: 122 GA----KRDALLLSFKDAKLSVVEYDPGTHDLKTLSLHYFEEPE---LRDGFVQNVHTPR 181

Query: 183 VKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLINLRDLD 242
           V+VDP GRC  +L+YG ++++L   +    L  + E     G  S+ + S Y+I++R LD
Sbjct: 182 VRVDPDGRCAAMLIYGTRLVVLPFRR--ESLAEEHEGLMGEGQRSSFLPS-YIIDVRALD 241

Query: 243 VK--HVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALSISTTLKQHPLIWS 302
            K  ++ D  F+HGY EP ++IL E   TW GRV+ +  TC + A+S++ T K HP+IWS
Sbjct: 242 EKLLNIIDLQFLHGYYEPTLLILFEPNQTWPGRVAVRQDTCSIVAISLNITQKVHPVIWS 301

Query: 303 ASNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCM-LALNNYAVSADSSQDMPRSN 362
            ++LP D  + LAVP PIGGV++ + NS+ Y +QS     +ALN+      +     +  
Sbjct: 302 LTSLPFDCTQALAVPKPIGGVVIFAVNSLLYLNQSVPPYGVALNSLTTGTTAFPLRTQEG 361

Query: 363 FNVELDAANATWLVNDVALLSTKTGELLLLALVYDG-RVVQRLDLSKSKASVLTSGIASI 422
             + LD A A ++  D  ++S K GE+ +L L+ DG R V+     K+ ASVLT+ + ++
Sbjct: 362 VRITLDCAQAAFISYDKMVISLKGGEIYVLTLITDGMRSVRAFHFDKAAASVLTTSMVTM 421

Query: 423 GNSLFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMR-----RSSSD 482
                FLGSRLG+SLL++++  +     AS+++ E  D E      KR+           
Sbjct: 422 EPGYLFLGSRLGNSLLLKYTEKL-QEPPASSVR-EAADKEEPPSKKKRVEPAVGWTGGKT 481

Query: 483 ALQDMVGGDELSLYGS-AANNTESAQKIFSFAVRDSLINIGPLKDFSYGLRINADPNATG 542
             QD V  DE+ +YGS A + T+ A   +SF V DS++NIGP  + + G           
Sbjct: 482 VPQDEV--DEIEVYGSEAQSGTQLA--TYSFEVCDSMLNIGPCANAAVGEPAFLSEEFQN 541

Query: 543 IAKQSNYELVCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVY-------HKNTR 602
            + + + E+V CSG+GKNGAL +L++SIRP+++T  ELPGC  +WTV         +  +
Sbjct: 542 -SPEPDLEIVVCSGYGKNGALSVLQKSIRPQVVTTFELPGCYDMWTVIAPVRKEEEETPK 601

Query: 603 GSIADSSRTVP---DDDEYHAYLIISLEARTMVLVTGELLTEVTESVDYFVHGRTIAAGN 662
               +   + P   +D   H +LI+S E  TM+L TG+ + E+  S  +   G T+ AGN
Sbjct: 602 AESTEQEPSAPKAEEDGRRHGFLILSREDSTMILQTGQEIMELDTS-GFATQGPTVFAGN 661

Query: 663 LFGRRRVIQVYESGARILDGSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMT 722
           +   R ++QV   G R+L+G          VN         G  ++  +++DPYV++   
Sbjct: 662 IGDNRYIVQVSPLGIRLLEG----------VNQLHFIPVDLGAPIVQCAVADPYVVIMSA 721

Query: 723 DGSIRLLVGDSSSCS-----VSVSAPAAFGSSKKCVSSCTLYQDKGFEPWLRMTSTDAWL 771
           +G + + +  S S       +++  P     SK  V +  LY+D        M +T++ L
Sbjct: 722 EGHVTMFLLKSDSYGGRHHRLALHKPPLHHQSK--VIALCLYRDVS-----GMFTTESRL 725

HSP 2 Score: 291.2 bits (744), Expect = 6.2e-77
Identity = 213/692 (30.78%), Postives = 331/692 (47.83%), Query Frame = 1

Query: 778  YCVACYDNGDLEIFDVPNFTSVFYVDKFVSGKSHLVDHQISDLQKSSEVDQNSQELISHG 837
            +C+   +NG +EI+ +P++  VF V  F  G+  LVD      Q +++ +   +E    G
Sbjct: 782  WCLLVRENGTMEIYQLPDWRLVFLVKNFPVGQRVLVDSSFG--QPTTQGEVRKEEATRQG 841

Query: 838  RNESSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFESTDSASKIDDSVSID 897
                 +   V EV +     + SRP+L  +  D  +L Y A+                 D
Sbjct: 842  -----ELPLVKEVLLVALGSRQSRPYLL-VHVDQELLIYEAF---------------PHD 901

Query: 898  NSVSSSNMSSSRLRNLRFLRVPLDIQGREDMPNGTLSR-----------------RLSIF 957
            + +   N+       +RF +VP +I  RE  P  +  +                 R   F
Sbjct: 902  SQLGQGNL------KVRFKKVPHNINFREKKPKPSKKKAEGCSTEEGSGGRGRVARFRYF 961

Query: 958  KNISGYQGLFLCGSRPAWFMVF-RERLRVHPQLCDGPIVAFAVLHNVNCNHGLIYVTSQV 1017
            ++I GY G+F+CG  P W +V  R  LR+HP   DGPI +FA  HNVNC  G +Y   Q 
Sbjct: 962  EDIYGYSGVFICGPSPHWLLVTGRGALRLHPMGIDGPIDSFAPFHNVNCPRGFLYFNRQG 1021

Query: 1018 PLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQV-LSSMVDQDVGHVENH--------NLS 1077
             L+            ++ P  +S     P+ ++ L         HVE+         N  
Sbjct: 1022 ELR-----------ISVLPAYLSYDAPWPVRKIPLRCTAHYVAYHVESKVYAVATSTNTP 1081

Query: 1078 ADEL-QQTYSVEEFEI-----RILEPEKSGGPWQTRATIAMHSSENALT-----IRVVTL 1137
               + + T   +EFE      R + P++     Q  + ++  +  NA         V  +
Sbjct: 1082 CTRIPRMTGEEKEFEAIERDDRYIHPQQEAFSIQLISPVSWEAIPNARIELEEWEHVTCM 1141

Query: 1138 LNTTTKENETL------LAVGTAYVQGEDVAARGRVLLFSVGKDA-DNSQTLVSE----V 1197
               + +  ET+      +A GT  +QGE+V  RGR+L+  V +   +  Q L       +
Sbjct: 1142 KTVSLRSEETVSGLKGYVAAGTCLMQGEEVTCRGRILIMDVIEVVPEPGQPLTKNKFKVL 1201

Query: 1198 YSKELKGAISALASLQGHLLIASGPKIILHKWTGAELNGIAFYDVPPLYVVSLNIVKNFI 1257
            Y KE KG ++AL    GHL+ A G KI L     +EL G+AF D   LY+  +  VKNFI
Sbjct: 1202 YEKEQKGPVTALCHCNGHLVSAIGQKIFLWSLRASELTGMAFIDT-QLYIHQMISVKNFI 1261

Query: 1258 LLGDIHKSIYFLSWKEQGAQLSLLAKDFGSLDCYATEFLIDGSTLSLTVSDDQKNIQIFY 1317
            L  D+ KSI  L ++E+   LSL+++D   L+ Y+ +F++D + L   VSD  +N+ ++ 
Sbjct: 1262 LAADVMKSISLLRYQEESKTLSLVSRDAKPLEVYSVDFMVDNAQLGFLVSDRDRNLMVYM 1321

Query: 1318 YAPKSTESWKGQKLLSRAEFHVGAHVTKFLRLQMLSTSSDKACSTVSDKTNRFALLFGTL 1377
            Y P++ ES+ G +LL RA+FHVGAHV  F R      +   +  +V  + N+    F TL
Sbjct: 1322 YLPEAKESFGGMRLLRRADFHVGAHVNTFWRTPCRGAAEGPSKKSVVWE-NKHITWFATL 1381

Query: 1378 DGSIGCIAPLDELTFRRLQSLQKKLGDAVPHVGGLNPRSFRQFHSNGKVHRRGPDSIVDC 1421
            DG IG + P+ E T+RRL  LQ  L   +PH  GLNPR+FR  H + ++ +    +++D 
Sbjct: 1382 DGGIGLLLPMQEKTYRRLLMLQNALTTMLPHHAGLNPRAFRMLHVDRRILQNAVRNVLDG 1431

BLAST of Cucsa.178510 vs. Swiss-Prot
Match: CPSF1_BOVIN (Cleavage and polyadenylation specificity factor subunit 1 OS=Bos taurus GN=CPSF1 PE=1 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 6.8e-92
Identity = 237/728 (32.55%), Postives = 376/728 (51.65%), Query Frame = 1

Query: 3   FAAYRMMHWPTGIE-NCDSAYITHSRADFVPAVTSHSDDLDSDWHPRRDIGPVPNLVVTA 62
           +A Y+  H PTG+E +    +  +S  + V A TS                         
Sbjct: 2   YAVYKQAHPPTGLEFSMYCNFFNNSERNLVVAGTS------------------------- 61

Query: 63  GNVLEVYVVRVLEEGGRESKSSGEVRRGGIMDGVSGASLELVCHYRLHGNVESMAILSSR 122
               ++YV R+  +   E+ +  +    G         LELV  +   GNV SMA +   
Sbjct: 62  ----QLYVYRLNRDS--EAPTKNDRSTDGKAHREHREKLELVASFSFFGNVMSMASVQLA 121

Query: 123 GGDGSKKRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWLHLKRGRESFARGPV 182
           G     KRD+++L F++AK+SV+E+D  TH L+T S+H F+ P+   L+ G       P 
Sbjct: 122 GA----KRDALLLSFKDAKLSVVEYDPGTHDLKTLSLHYFEEPE---LRDGFVQNVHTPR 181

Query: 183 VKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLINLRDLD 242
           V+VDP GRC  +L+YG ++++L   +    L  + E     G  S+ + S Y+I++R LD
Sbjct: 182 VRVDPDGRCAAMLIYGTRLVVLPFRR--ESLAEEHEGLVGEGQRSSFLPS-YIIDVRALD 241

Query: 243 VK--HVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALSISTTLKQHPLIWS 302
            K  ++ D  F+HGY EP ++IL E   TW GRV+ +  TC + A+S++ T K HP+IWS
Sbjct: 242 EKLLNIVDLQFLHGYYEPTLLILFEPNQTWPGRVAVRQDTCSIVAISLNITQKVHPVIWS 301

Query: 303 ASNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCM-LALNNYAVSADSSQDMPRSN 362
            ++LP D  + LAVP PIGGV++ + NS+ Y +QS     +ALN+      +     +  
Sbjct: 302 LTSLPFDCTQALAVPKPIGGVVIFAVNSLLYLNQSVPPYGVALNSLTTGTTAFPLRTQEG 361

Query: 363 FNVELDAANATWLVNDVALLSTKTGELLLLALVYDG-RVVQRLDLSKSKASVLTSGIASI 422
             + LD A A ++  D  ++S K GE+ +L L+ DG R V+     K+ ASVLT+ + ++
Sbjct: 362 VRITLDCAQAAFISYDKMVISLKGGEIYVLTLITDGMRSVRAFHFDKAAASVLTTSMVTM 421

Query: 423 GNSLFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMRRS-----SSD 482
                FLGSRLG+SLL++++  +      ++   E  D E      KR+  +     S  
Sbjct: 422 EPGYLFLGSRLGNSLLLKYTEKLQEP--PASTAREAADKEEPPSKKKRVDATTGWSGSKS 481

Query: 483 ALQDMVGGDELSLYGS-AANNTESAQKIFSFAVRDSLINIGPLKDFSYGLRINADPNATG 542
             QD V  DE+ +YGS A + T+ A   +SF V DS++NIGP  + + G           
Sbjct: 482 VPQDEV--DEIEVYGSEAQSGTQLA--TYSFEVCDSILNIGPCANAAMGEPAFLSEEFQN 541

Query: 543 IAKQSNYELVCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTV-------YHKNTR 602
            + + + E+V CSG+GKNGAL +L++SIRP+++T  ELPGC  +WTV         +  +
Sbjct: 542 -SPEPDLEIVVCSGYGKNGALSVLQKSIRPQVVTTFELPGCYDMWTVIAPVRKEQEETLK 601

Query: 603 GSIADSSRTVP---DDDEYHAYLIISLEARTMVLVTGELLTEVTESVDYFVHGRTIAAGN 662
           G   +     P   DD   H +LI+S E  TM+L TG+ + E+  S  +   G T+ AGN
Sbjct: 602 GEGTEPEPGAPEAEDDGRRHGFLILSREDSTMILQTGQEIMELDAS-GFATQGPTVFAGN 661

Query: 663 LFGRRRVIQVYESGARILDGSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMT 710
           +   R ++QV   G R+L+G          VN         G  ++  +++DPYV++   
Sbjct: 662 IGDNRYIVQVSPLGIRLLEG----------VNQLHFIPVDLGSPIVQCAVADPYVVIMSA 670

HSP 2 Score: 287.0 bits (733), Expect = 1.2e-75
Identity = 214/692 (30.92%), Postives = 330/692 (47.69%), Query Frame = 1

Query: 778  YCVACYDNGDLEIFDVPNFTSVFYVDKFVSGKSHLVDHQISDLQKSSEVDQNSQELISHG 837
            +C+   +NG +EI+ +P++  VF V  F  G+  LVD      Q +++ +   +E    G
Sbjct: 785  WCLLVRENGAMEIYQLPDWRLVFLVKNFPVGQRVLVDSSFG--QPTTQGEARKEEATRQG 844

Query: 838  RNESSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFESTDSASKIDDSVSID 897
                 +   V EV +     +  RP+L  +  D  +L Y A+                 D
Sbjct: 845  -----ELPLVKEVLLVALGSRQRRPYLL-VHVDQELLIYEAFPH---------------D 904

Query: 898  NSVSSSNMSSSRLRNLRFLRVPLDIQGREDMPN-------------GTLSR----RLSIF 957
            + +   N+       +RF +VP +I  RE  P              GT  R    R   F
Sbjct: 905  SQLGQGNLK------VRFKKVPHNINFREKKPKPSKKKAEGGSTEEGTGPRGRVARFRYF 964

Query: 958  KNISGYQGLFLCGSRPAWFMVF-RERLRVHPQLCDGPIVAFAVLHNVNCNHGLIYVTSQV 1017
            ++I GY G+F+CG  P W +V  R  LR+HP   DGPI +FA  HN+NC  G +Y   Q 
Sbjct: 965  EDIYGYSGVFICGPSPHWLLVTGRGALRLHPMGIDGPIDSFAPFHNINCPRGFLYFNRQG 1024

Query: 1018 PLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQV-LSSMVDQDVGHVENHNLSADEL---- 1077
             L+            ++ P  +S     P+ ++ L         HVE+   +        
Sbjct: 1025 ELR-----------ISVLPAYLSYDAPWPVRKIPLRCTAHYVAYHVESKVYAVATSTSTP 1084

Query: 1078 -----QQTYSVEEFEI-----RILEPEKSGGPWQTRATIAMHSSENALT-----IRVVTL 1137
                 + T   +EFE      R + P++     Q  + ++  +  NA         V  +
Sbjct: 1085 CTRVPRMTGEEKEFETIERDERYVHPQQEAFCIQLISPVSWEAIPNARIELEEWEHVTCM 1144

Query: 1138 LNTTTKENETL------LAVGTAYVQGEDVAARGRVLLFSVGKDA-DNSQTLVSE----V 1197
               + +  ET+      +A GT  +QGE+V  RGR+L+  V +   +  Q L       +
Sbjct: 1145 KTVSLRSEETVSGLKGYVAAGTCLMQGEEVTCRGRILIMDVIEVVPEPGQPLTKNKFKVL 1204

Query: 1198 YSKELKGAISALASLQGHLLIASGPKIILHKWTGAELNGIAFYDVPPLYVVSLNIVKNFI 1257
            Y KE KG ++AL    GHL+ A G KI L     +EL G+AF D   LY+  +  VKNFI
Sbjct: 1205 YEKEQKGPVTALCHCNGHLVSAIGQKIFLWSLRASELTGMAFIDT-QLYIHQMISVKNFI 1264

Query: 1258 LLGDIHKSIYFLSWKEQGAQLSLLAKDFGSLDCYATEFLIDGSTLSLTVSDDQKNIQIFY 1317
            L  D+ KSI  L ++E+   LSL+++D   L+ Y+ +F++D + L   VSD  +N+ ++ 
Sbjct: 1265 LAADVMKSISLLRYQEESKTLSLVSRDAKPLEVYSVDFMVDNAQLGFLVSDRDRNLMVYM 1324

Query: 1318 YAPKSTESWKGQKLLSRAEFHVGAHVTKFLRLQMLSTSSDKACSTVSDKTNRFALLFGTL 1377
            Y P++ ES+ G +LL RA+FHVGAHV  F R      +   +  +V  + N+    F TL
Sbjct: 1325 YLPEAKESFGGMRLLRRADFHVGAHVNTFWRTPCRGAAEGPSKKSVVWE-NKHITWFATL 1384

Query: 1378 DGSIGCIAPLDELTFRRLQSLQKKLGDAVPHVGGLNPRSFRQFHSNGKVHRRGPDSIVDC 1421
            DG IG + P+ E T+RRL  LQ  L   +PH  GLNPR+FR  H + +V +    +++D 
Sbjct: 1385 DGGIGLLLPMQEKTYRRLLMLQNALTTMLPHHAGLNPRAFRMLHVDRRVLQNAVRNVLDG 1434

BLAST of Cucsa.178510 vs. TrEMBL
Match: A0A0A0LKI9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G074280 PE=4 SV=1)

HSP 1 Score: 2820.8 bits (7311), Expect = 0.0e+00
Identity = 1427/1452 (98.28%), Postives = 1427/1452 (98.28%), Query Frame = 1

Query: 1    MSFAAYRMMHWPTGIENCDSAYITHSRADFVPAVTSHSDDLDSDWHPRRDIGPVPNLVVT 60
            MSFAAYRMMHWPTGIENCDSAYITHSRADFVPAVTSHSDDLDSDWHPRRDIGPVPNLVVT
Sbjct: 1    MSFAAYRMMHWPTGIENCDSAYITHSRADFVPAVTSHSDDLDSDWHPRRDIGPVPNLVVT 60

Query: 61   AGNVLEVYVVRVLEEGGRESKSSGEVRRGGIMDGVSGASLELVCHYRLHGNVESMAILSS 120
            AGNVLEVYVVRVLEEGGRESKSSGEVRRGGIMDGVSGASLELVCHYRLHGNVESMAILSS
Sbjct: 61   AGNVLEVYVVRVLEEGGRESKSSGEVRRGGIMDGVSGASLELVCHYRLHGNVESMAILSS 120

Query: 121  RGGDGSKKRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWLHLKRGRESFARGP 180
            RGGDGSKKRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWLHLKRGRESFARGP
Sbjct: 121  RGGDGSKKRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWLHLKRGRESFARGP 180

Query: 181  VVKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLINLRDL 240
            VVKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLINLRDL
Sbjct: 181  VVKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLINLRDL 240

Query: 241  DVKHVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALSISTTLKQHPLIWSA 300
            DVKHVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALSISTTLKQHPLIWSA
Sbjct: 241  DVKHVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALSISTTLKQHPLIWSA 300

Query: 301  SNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCMLALNNYAVSADSSQDMPRSNFN 360
            SNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCMLALNNYAVSADSSQDMPRSNFN
Sbjct: 301  SNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCMLALNNYAVSADSSQDMPRSNFN 360

Query: 361  VELDAANATWLVNDVALLSTKTGELLLLALVYDGRVVQRLDLSKSKASVLTSGIASIGNS 420
            VELDAANATWLVNDVALLSTKTGELLLLALVYDGRVVQRLDLSKSKASVLTSGIASIGNS
Sbjct: 361  VELDAANATWLVNDVALLSTKTGELLLLALVYDGRVVQRLDLSKSKASVLTSGIASIGNS 420

Query: 421  LFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMRRSSSDALQDMVGG 480
            LFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMRRSSSDALQDMVGG
Sbjct: 421  LFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMRRSSSDALQDMVGG 480

Query: 481  DELSLYGSAANNTESAQKIFSFAVRDSLINIGPLKDFSYGLRINADPNATGIAKQSNYEL 540
            DELSLYGSAANNTESAQKIFSFAVRDSLINIGPLKDFSYGLRINADPNATGIAKQSNYEL
Sbjct: 481  DELSLYGSAANNTESAQKIFSFAVRDSLINIGPLKDFSYGLRINADPNATGIAKQSNYEL 540

Query: 541  VCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVYHKNTRGSIADSSRTVPDDDEY 600
            VCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVYHKNTRGSIADSSR VPDDDEY
Sbjct: 541  VCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVYHKNTRGSIADSSRMVPDDDEY 600

Query: 601  HAYLIISLEARTMVLVTGELLTEVTESVDYFVHGRTIAAGNLFGRRRVIQVYESGARILD 660
            HAYLIISLEARTMVLVTGELLTEVTESVDYFVHGRTIAAGNLFGRRRVIQVYESGARILD
Sbjct: 601  HAYLIISLEARTMVLVTGELLTEVTESVDYFVHGRTIAAGNLFGRRRVIQVYESGARILD 660

Query: 661  GSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMTDGSIRLLVGDSSSCSVSVS 720
            GSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMTDGSIRLLVGDSSSCSVSVS
Sbjct: 661  GSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMTDGSIRLLVGDSSSCSVSVS 720

Query: 721  APAAFGSSKKCVSSCTLYQDKGFEPWLRMTSTDAWLSTGVGETIDGTDGSLQDQGDIYCV 780
            APAAFGSSKKCVSSCTLYQDKG EPWLRMTSTDAWLSTGVGETIDGTDGSLQDQGDIYCV
Sbjct: 721  APAAFGSSKKCVSSCTLYQDKGIEPWLRMTSTDAWLSTGVGETIDGTDGSLQDQGDIYCV 780

Query: 781  ACYDNGDLEIFDVPNFTSVFYVDKFVSGKSHLVDHQISDLQKSSEVDQNSQELISHGRNE 840
            ACYDNGDLEIFDVPNFTSVFYVDKFVSGKSHLVDHQISDLQKSSEVDQNSQELISHGRNE
Sbjct: 781  ACYDNGDLEIFDVPNFTSVFYVDKFVSGKSHLVDHQISDLQKSSEVDQNSQELISHGRNE 840

Query: 841  SSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFESTDSASKIDDSVSIDNSV 900
            SSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFESTDSASKIDDSVSIDNSV
Sbjct: 841  SSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFESTDSASKIDDSVSIDNSV 900

Query: 901  SSSNMSSSRLRNLRFLRVPLDIQGREDMPNGTLSRRLSIFKNISGYQGLFLCGSRPAWFM 960
            SSSNMSSSRLRNLRFLRVPLDIQGREDMPNGTLS RLSIFKNISGYQGLFLCGSRPAWFM
Sbjct: 901  SSSNMSSSRLRNLRFLRVPLDIQGREDMPNGTLSCRLSIFKNISGYQGLFLCGSRPAWFM 960

Query: 961  VFRERLRVHPQLCDGPIVAFAVLHNVNCNHGLIYVTSQ---------------------- 1020
            VFRERLRVHPQLCDGPIVAFAVLHNVNCNHGLIYVTSQ                      
Sbjct: 961  VFRERLRVHPQLCDGPIVAFAVLHNVNCNHGLIYVTSQGVLKICQLPSTSNYDNYWPVQK 1020

Query: 1021 VPLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQVLSSMVDQDVGHVENHNLSADELQQTY 1080
            VPLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQVLSSMVDQDVGHVENHNLSADELQQTY
Sbjct: 1021 VPLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQVLSSMVDQDVGHVENHNLSADELQQTY 1080

Query: 1081 SVEEFEIRILEPEKSGGPWQTRATIAMHSSENALTIRVVTLLNTTTKENETLLAVGTAYV 1140
            SVEEFEIRILEPEKSGGPWQTRATIAMHSSENALTIRVVTLLNTTTKENETLLAVGTAYV
Sbjct: 1081 SVEEFEIRILEPEKSGGPWQTRATIAMHSSENALTIRVVTLLNTTTKENETLLAVGTAYV 1140

Query: 1141 QGEDVAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALASLQGHLLIASGPKIILH 1200
            QGEDVAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALASLQGHLLIASGPKIILH
Sbjct: 1141 QGEDVAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALASLQGHLLIASGPKIILH 1200

Query: 1201 KWTGAELNGIAFYDVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLLAKDFGS 1260
            KWTGAELNGIAFYDVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLLAKDFGS
Sbjct: 1201 KWTGAELNGIAFYDVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLLAKDFGS 1260

Query: 1261 LDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTESWKGQKLLSRAEFHVGAHVTKFL 1320
            LDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTESWKGQKLLSRAEFHVGAHVTKFL
Sbjct: 1261 LDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTESWKGQKLLSRAEFHVGAHVTKFL 1320

Query: 1321 RLQMLSTSSDKACSTVSDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKLGDAVP 1380
            RLQMLSTSSDKACSTVSDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKLGDAVP
Sbjct: 1321 RLQMLSTSSDKACSTVSDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKLGDAVP 1380

Query: 1381 HVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELLCHYEMLPLEEQLDIAHQIGTTRSQILS 1431
            HVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELLCHYEMLPLEEQLDIAHQIGTTRSQILS
Sbjct: 1381 HVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELLCHYEMLPLEEQLDIAHQIGTTRSQILS 1440

BLAST of Cucsa.178510 vs. TrEMBL
Match: A0A061G7F2_THECC (Cleavage and polyadenylation specificity factor 160 isoform 1 OS=Theobroma cacao GN=TCM_014995 PE=4 SV=1)

HSP 1 Score: 2331.2 bits (6040), Expect = 0.0e+00
Identity = 1156/1457 (79.34%), Postives = 1289/1457 (88.47%), Query Frame = 1

Query: 1    MSFAAYRMMHWPTGIENCDSAYITHSRADFVPAVT-SHSDDLDSDWHPRRDIGPVPNLVV 60
            MS+AAY+MMHWPTGIENC S ++TH RADF P +  + ++DL+S+W  RR IGPVPNL+V
Sbjct: 1    MSYAAYKMMHWPTGIENCASGFVTHCRADFTPQIPLNQTEDLESEWPARRGIGPVPNLIV 60

Query: 61   TAGNVLEVYVVRVLEEGGRESKSSGEVRRGGIMDGVSGASLELVCHYRLHGNVESMAILS 120
            TA N+LE+YVVRV EEG RE+++S EV+RGG++DGVSG SLELVC+YRLHGNVESMA+LS
Sbjct: 61   TAANLLEIYVVRVQEEGRREARNSTEVKRGGVLDGVSGVSLELVCNYRLHGNVESMAVLS 120

Query: 121  SRGGDGSKKRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWLHLKRGRESFARG 180
              GGDGS++RDSIIL F++AKISVLEFDDS H LRT+SMHCF+GP+WLHLKRGRESFARG
Sbjct: 121  IGGGDGSRRRDSIILAFKDAKISVLEFDDSIHGLRTTSMHCFEGPEWLHLKRGRESFARG 180

Query: 181  PVVKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLINLRD 240
            P+VKVDPQGRCGGVLVY LQMIILKASQAGSG V +D+AFG+ GA+SARVESSY+INLRD
Sbjct: 181  PLVKVDPQGRCGGVLVYDLQMIILKASQAGSGFVGEDDAFGSGGAVSARVESSYIINLRD 240

Query: 241  LDVKHVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALSISTTLKQHPLIWS 300
            LDVKH+KDF+FVHGYIEPVMVILHE+ELTWAGRVSWKHHTCM+SALSISTTLKQHPLIWS
Sbjct: 241  LDVKHIKDFIFVHGYIEPVMVILHERELTWAGRVSWKHHTCMISALSISTTLKQHPLIWS 300

Query: 301  ASNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCMLALNNYAVSADSSQDMPRSNF 360
            A NLPHDAYKLLAVPSPIGGVLVISAN+IHY+SQSASC LALNNYA+S D+SQD+PRSNF
Sbjct: 301  AVNLPHDAYKLLAVPSPIGGVLVISANTIHYHSQSASCALALNNYAISVDNSQDLPRSNF 360

Query: 361  NVELDAANATWLVNDVALLSTKTGELLLLALVYDGRVVQRLDLSKSKASVLTSGIASIGN 420
            +VELDAANATWL+NDVALLSTKTGELLLL L+YDGRVVQRLDLSKSKASVLTS I +IGN
Sbjct: 361  SVELDAANATWLLNDVALLSTKTGELLLLTLIYDGRVVQRLDLSKSKASVLTSDITTIGN 420

Query: 421  SLFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMRRSSSDALQDMVG 480
            SLFFLGSRLGDSLLVQFS G G S L S LK+E GDIE D   AKR+RRSSSDALQDMVG
Sbjct: 421  SLFFLGSRLGDSLLVQFSGGSGVSALPSGLKEEVGDIEGDVPLAKRLRRSSSDALQDMVG 480

Query: 481  GDELSLYGSAANNTESAQKIFSFAVRDSLINIGPLKDFSYGLRINADPNATGIAKQSNYE 540
            G+ELSLYGSA NNTESAQK F FAVRDSL N+GPLKDFSYGLRINAD NATGIAKQSNYE
Sbjct: 481  GEELSLYGSAPNNTESAQKTFLFAVRDSLTNVGPLKDFSYGLRINADVNATGIAKQSNYE 540

Query: 541  LVCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVYHKNTRGSIADSSRTVPDDDE 600
            LVCCSGHGKNGALC+LRQSIRPEMITEVEL GCKGIWTVYHK+TR   AD S+   DDDE
Sbjct: 541  LVCCSGHGKNGALCVLRQSIRPEMITEVELTGCKGIWTVYHKSTRSHSADLSKVTDDDDE 600

Query: 601  YHAYLIISLEARTMVLVTGELLTEVTESVDYFVHGRTIAAGNLFGRRRVIQVYESGARIL 660
            YHAYLIISLEARTMVL T +LLTEVTESVDY+V GRTIAAGNLFGRRRV+QVYE GARIL
Sbjct: 601  YHAYLIISLEARTMVLETADLLTEVTESVDYYVQGRTIAAGNLFGRRRVVQVYERGARIL 660

Query: 661  DGSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMTDGSIRLLVGDSSSCSVSV 720
            DGSFMTQ+L++    +ES   SE  TV+S SI+DPYVLL MTDGSI LLVGD ++C+VS+
Sbjct: 661  DGSFMTQELSIPSPNSESSPGSENSTVISVSIADPYVLLRMTDGSILLLVGDPATCTVSI 720

Query: 721  SAPAAFGSSKKCVSSCTLYQDKGFEPWLRMTSTDAWLSTGVGETIDGTDGSLQDQGDIYC 780
            + P AF  SKK VS+CTLY DKG EPWLR  STDAWLSTGVGE+IDG DG   DQGDIYC
Sbjct: 721  NTPTAFEGSKKMVSACTLYHDKGPEPWLRKASTDAWLSTGVGESIDGADGGPHDQGDIYC 780

Query: 781  VACYDNGDLEIFDVPNFTSVFYVDKFVSGKSHLVDHQISDLQKSSE--VDQNSQELISHG 840
            V CY++G LEIFDVPNF  VF ++KF SG++ LVD    +  K SE  ++++S+EL   G
Sbjct: 781  VVCYESGALEIFDVPNFNCVFSMEKFASGRTRLVDAYTLESSKDSEKVINKSSEELTGQG 840

Query: 841  RNESSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFESTDSASKIDDSVSID 900
            R E+ QN+KV+E+AMQRWS  HSRPFLFGILTDGTILCYHAYLFE +++ASK++DSV   
Sbjct: 841  RKENVQNLKVVELAMQRWSANHSRPFLFGILTDGTILCYHAYLFEGSENASKVEDSVVAQ 900

Query: 901  NSVSSSNMSSSRLRNLRFLRVPLDIQGREDMPNGTLSRRLSIFKNISGYQGLFLCGSRPA 960
            NSV  SN+++SRLRNLRF+R+PLD   RE+M NGTLS+R++IFKNISGYQG FL GSRPA
Sbjct: 901  NSVGLSNINASRLRNLRFIRIPLDAYTREEMSNGTLSQRITIFKNISGYQGFFLSGSRPA 960

Query: 961  WFMVFRERLRVHPQLCDGPIVAFAVLHNVNCNHGLIYVTSQ------------------- 1020
            WFMVFRERLRVHPQLCDG IVAF VLHNVNCNHG IYVTSQ                   
Sbjct: 961  WFMVFRERLRVHPQLCDGSIVAFTVLHNVNCNHGFIYVTSQGILKICQIPSASNYDNYWP 1020

Query: 1021 ---VPLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQVLSSMVDQDVGH-VENHNLSADEL 1080
               +PL+GTPHQVTYF E+NLYP+I+S PV KP+NQVLSS+VDQ+VGH ++NHNLS+DEL
Sbjct: 1021 VQKIPLRGTPHQVTYFAERNLYPIIVSVPVHKPVNQVLSSLVDQEVGHQMDNHNLSSDEL 1080

Query: 1081 QQTYSVEEFEIRILEPEKSGGPWQTRATIAMHSSENALTIRVVTLLNTTTKENETLLAVG 1140
            Q+TY+V+EFE+RILEPEKSGGPW+T+ATI M SSENALT+RVVTL NTTTKENE+LLA+G
Sbjct: 1081 QRTYTVDEFEVRILEPEKSGGPWETKATIPMQSSENALTVRVVTLFNTTTKENESLLAIG 1140

Query: 1141 TAYVQGEDVAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALASLQGHLLIASGPK 1200
            TAY+QGEDVAARGRV+L S+G++ DN Q LVSEVYSKELKGAISALASLQGHLLIASGPK
Sbjct: 1141 TAYIQGEDVAARGRVILCSIGRNTDNLQNLVSEVYSKELKGAISALASLQGHLLIASGPK 1200

Query: 1201 IILHKWTGAELNGIAFYDVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLLAK 1260
            IILH WTG+ELNGIAFYD PPLYVVSLNIVKNFILLGD+HKSIYFLSWKEQGAQLSLLAK
Sbjct: 1201 IILHNWTGSELNGIAFYDAPPLYVVSLNIVKNFILLGDVHKSIYFLSWKEQGAQLSLLAK 1260

Query: 1261 DFGSLDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTESWKGQKLLSRAEFHVGAHV 1320
            DFGSLDC+ATEFLIDGSTLSL VSD+QKNIQIFYYAPK +ESWKGQKLLSRAEFHVGAHV
Sbjct: 1261 DFGSLDCFATEFLIDGSTLSLMVSDEQKNIQIFYYAPKMSESWKGQKLLSRAEFHVGAHV 1320

Query: 1321 TKFLRLQMLSTSSDKACSTV-SDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKL 1380
            TKFLRLQMLSTSSD+  +T  SDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKL
Sbjct: 1321 TKFLRLQMLSTSSDRTSATAGSDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKL 1380

Query: 1381 GDAVPHVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELLCHYEMLPLEEQLDIAHQIGTTR 1431
             DAVPHV GLNPRSFRQFHSNGK HR GPDSIVDCELLCHYEMLPLEEQLDIAHQIGTTR
Sbjct: 1381 VDAVPHVAGLNPRSFRQFHSNGKAHRPGPDSIVDCELLCHYEMLPLEEQLDIAHQIGTTR 1440

BLAST of Cucsa.178510 vs. TrEMBL
Match: M5X6F1_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa000211mg PE=4 SV=1)

HSP 1 Score: 2308.5 bits (5981), Expect = 0.0e+00
Identity = 1151/1459 (78.89%), Postives = 1282/1459 (87.87%), Query Frame = 1

Query: 1    MSFAAYRMMHWPTGIENCDSAYITHSRADFVPAVTS-HSDDLDSDWHP-RRDIGPVPNLV 60
            MSFAAY+MMHWPTGIENC S +I+HSR+DFVP +    ++DL+S+W   RR+IGP+P+LV
Sbjct: 1    MSFAAYKMMHWPTGIENCASGFISHSRSDFVPRIPPIQTEDLESEWPTSRREIGPIPDLV 60

Query: 61   VTAGNVLEVYVVRVLEEGG-RESKSSGEVRRGGIMDGVSGASLELVCHYRLHGNVESMAI 120
            VTAGNVLEVYVVRV EE G R  ++SGE +RGG+MDGVSGASLELVCHYRLHGNV +MA+
Sbjct: 61   VTAGNVLEVYVVRVQEEDGTRGPRASGEPKRGGLMDGVSGASLELVCHYRLHGNVVTMAV 120

Query: 121  LSSRGGDGSKKRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWLHLKRGRESFA 180
            LSS GGDGS++RDSIIL F++AKISVLEFDDS H LRTSSMHCF+GP+WLHL+RGRESFA
Sbjct: 121  LSSGGGDGSRRRDSIILTFEDAKISVLEFDDSIHGLRTSSMHCFEGPEWLHLRRGRESFA 180

Query: 181  RGPVVKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLINL 240
            RGP+VKVDPQGRCG +LVYGLQMIILKASQ GSGLV DD++FG+ GAIS+R+ESSY++NL
Sbjct: 181  RGPLVKVDPQGRCGSILVYGLQMIILKASQGGSGLVGDDDSFGSGGAISSRIESSYIVNL 240

Query: 241  RDLDVKHVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALSISTTLKQHPLI 300
            RD+D+KHVKDF F+HGYIEPVMVILHE+ELTWAGRVSWKHHTCM+SALSISTTLKQHPLI
Sbjct: 241  RDMDMKHVKDFTFLHGYIEPVMVILHERELTWAGRVSWKHHTCMISALSISTTLKQHPLI 300

Query: 301  WSASNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCMLALNNYAVSADSSQDMPRS 360
            WSA NLPHDAYKLLAVPSPIGGVLVISANSIHY+SQSASC LALN+YAVSAD+SQ+MPRS
Sbjct: 301  WSAVNLPHDAYKLLAVPSPIGGVLVISANSIHYHSQSASCALALNSYAVSADNSQEMPRS 360

Query: 361  NFNVELDAANATWLVNDVALLSTKTGELLLLALVYDGRVVQRLDLSKSKASVLTSGIASI 420
            +F VELD ANATWL+NDVALLSTKTGELLLL LVYDGRVVQRLDLSKSKASVLTSGI  +
Sbjct: 361  SFTVELDTANATWLLNDVALLSTKTGELLLLTLVYDGRVVQRLDLSKSKASVLTSGITKV 420

Query: 421  GNSLFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMRRSSSDALQDM 480
            GNSLFFLGSRLGDSLLVQF+CGVG S L+S++KDE GDIE DA  AKR+R SSSDALQDM
Sbjct: 421  GNSLFFLGSRLGDSLLVQFTCGVGGSVLSSDMKDEVGDIEGDAPLAKRLRMSSSDALQDM 480

Query: 481  VGGDELSLYGSAANNTESAQKIFSFAVRDSLINIGPLKDFSYGLRINADPNATGIAKQSN 540
            V G+ELSLYGSA NN ESAQK FSFAVRDSLIN+GPLKDFSYGLRINAD NATGIAKQSN
Sbjct: 481  VSGEELSLYGSAPNNAESAQKSFSFAVRDSLINVGPLKDFSYGLRINADANATGIAKQSN 540

Query: 541  YELVCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVYHKNTRGSIADSSRTVPDD 600
            YELVCCSGHGKNGALC+LRQSIRPEMITEVELPGCKGIWTVYHKN RG  ADSS+    D
Sbjct: 541  YELVCCSGHGKNGALCVLRQSIRPEMITEVELPGCKGIWTVYHKNARGHNADSSKIAASD 600

Query: 601  DEYHAYLIISLEARTMVLVTGELLTEVTESVDYFVHGRTIAAGNLFGRRRVIQVYESGAR 660
            DE+HAYLIISLEARTMVL T +LL+EVTESVDYFV GRTIAAGNLFGRRRV+QVYE GAR
Sbjct: 601  DEFHAYLIISLEARTMVLETADLLSEVTESVDYFVQGRTIAAGNLFGRRRVVQVYERGAR 660

Query: 661  ILDGSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMTDGSIRLLVGDSSSCSV 720
            ILDGSFMTQDL+   + +E G+ SE  TVLS SI DPYVLL M+DG IRLLVGD S C+V
Sbjct: 661  ILDGSFMTQDLSFGTSNSEMGSGSESSTVLSVSIVDPYVLLRMSDGGIRLLVGDPSLCTV 720

Query: 721  SVSAPAAFGSSKKCVSSCTLYQDKGFEPWLRMTSTDAWLSTGVGETIDGTDGSLQDQGDI 780
            S S PAAF SSKK +S+CTLY DKG EPWLR TSTDAWLSTG+ E IDG DG   DQGD+
Sbjct: 721  STSIPAAFESSKKSISACTLYHDKGPEPWLRKTSTDAWLSTGIDEAIDGADGVSHDQGDV 780

Query: 781  YCVACYDNGDLEIFDVPNFTSVFYVDKFVSGKSHLVDHQISDLQKSSE--VDQNSQELIS 840
            YCV CY++G LEIFDVPNF  VF VDKFVSG +HL+D  + D  K  +  ++++S+E+  
Sbjct: 781  YCVVCYESGSLEIFDVPNFNCVFSVDKFVSGNAHLIDTLMRDPPKDPQKLINKSSEEVSG 840

Query: 841  HGRNESSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFESTDSASKIDDSVS 900
             GR E+ QNMKV+E+AMQRWSGQHSRPFLFGIL DG ILCYHAYLFE  ++ASK +DS S
Sbjct: 841  QGRKENIQNMKVVELAMQRWSGQHSRPFLFGILNDGMILCYHAYLFEGPETASKTEDSAS 900

Query: 901  IDNSVSSSNMSSSRLRNLRFLRVPLDIQGREDMPNGTLSRRLSIFKNISGYQGLFLCGSR 960
              N+   SN+S+SRLRNLRF+RVPLD   ++D  N T  +R++IFKNI+GYQGLFL GSR
Sbjct: 901  AQNTTGVSNLSASRLRNLRFVRVPLDTYAKKDTSNETSCQRMTIFKNIAGYQGLFLSGSR 960

Query: 961  PAWFMVFRERLRVHPQLCDGPIVAFAVLHNVNCNHGLIYVTSQ----------------- 1020
            PAWFMVFRERLR+HPQLCDG +VA  VLHNVNCNHGLIYVTSQ                 
Sbjct: 961  PAWFMVFRERLRIHPQLCDGSVVAVTVLHNVNCNHGLIYVTSQGILKICQLPPITSYDNY 1020

Query: 1021 -----VPLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQVLSSMVDQDVGH-VENHNLSAD 1080
                 +PLKGTPHQVTYF EKNLYP+I+S PV KPLNQVLSS+VDQ+VGH VENHNLS+D
Sbjct: 1021 WPVQKIPLKGTPHQVTYFAEKNLYPLIVSVPVHKPLNQVLSSLVDQEVGHQVENHNLSSD 1080

Query: 1081 ELQQTYSVEEFEIRILEPEKSGGPWQTRATIAMHSSENALTIRVVTLLNTTTKENETLLA 1140
            EL +TYSV+EFEIRI+EP+KSGGPWQT+ATI M +SENALT+RVVTL NTTTKENETLLA
Sbjct: 1081 ELHRTYSVDEFEIRIMEPDKSGGPWQTKATIPMQTSENALTVRVVTLFNTTTKENETLLA 1140

Query: 1141 VGTAYVQGEDVAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALASLQGHLLIASG 1200
            +GTAYVQGEDVA RGRVLLFS GK ADN+QTLVSEVYSKELKGAISALASLQGHLLIASG
Sbjct: 1141 IGTAYVQGEDVAGRGRVLLFSAGKSADNTQTLVSEVYSKELKGAISALASLQGHLLIASG 1200

Query: 1201 PKIILHKWTGAELNGIAFYDVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLL 1260
            PKIILHKW G ELNG+AF+DVPPLYVVSLNIVKNFILLGD+HKSIYFLSWKEQGAQL+LL
Sbjct: 1201 PKIILHKWNGTELNGVAFFDVPPLYVVSLNIVKNFILLGDVHKSIYFLSWKEQGAQLTLL 1260

Query: 1261 AKDFGSLDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTESWKGQKLLSRAEFHVGA 1320
            AKDFG+LDC+ATEFLIDGSTLSL V+D+QKNIQIFYYAPK +ESWKGQKLLSRAEFHVG 
Sbjct: 1261 AKDFGNLDCFATEFLIDGSTLSLVVADEQKNIQIFYYAPKMSESWKGQKLLSRAEFHVGT 1320

Query: 1321 HVTKFLRLQMLSTSSDK-ACSTVSDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQK 1380
            HVTKFLRLQMLSTSSD+   +  SDKTNR+ALLFGTLDGSIGCIAPLDELTFRRLQSLQK
Sbjct: 1321 HVTKFLRLQMLSTSSDRTGTNPGSDKTNRYALLFGTLDGSIGCIAPLDELTFRRLQSLQK 1380

Query: 1381 KLGDAVPHVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELLCHYEMLPLEEQLDIAHQIGT 1431
            KL DAV HV GLNPR+FRQF SNGK HR GPD+IVDCELL HYEMLPLEEQL+IA+QIGT
Sbjct: 1381 KLVDAVHHVAGLNPRAFRQFQSNGKAHRPGPDTIVDCELLSHYEMLPLEEQLEIANQIGT 1440

BLAST of Cucsa.178510 vs. TrEMBL
Match: A0A0D2RRD1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_006G065300 PE=4 SV=1)

HSP 1 Score: 2285.8 bits (5922), Expect = 0.0e+00
Identity = 1134/1456 (77.88%), Postives = 1273/1456 (87.43%), Query Frame = 1

Query: 1    MSFAAYRMMHWPTGIENCDSAYITHSRADFVPAVT-SHSDDLDSDWHPRRDIGPVPNLVV 60
            MS+AAY+MMHWPTGIENC S ++T+ RADF P +  +H++DL+SDW  RR IGPVPNL+V
Sbjct: 1    MSYAAYKMMHWPTGIENCASGFVTNCRADFTPQIPLNHTEDLESDWSSRRGIGPVPNLIV 60

Query: 61   TAGNVLEVYVVRVLEEGGRESKSSGEVRRGGIMDGVSGASLELVCHYRLHGNVESMAILS 120
            TA NVLE+YVVRV EEG RE+++S EV+RGGIMDGVS  SLELVC YRLHGNVESMA+LS
Sbjct: 61   TAANVLELYVVRVQEEGTREARNSTEVKRGGIMDGVSAVSLELVCSYRLHGNVESMAVLS 120

Query: 121  SRGGDGSKKRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWLHLKRGRESFARG 180
              GGD S++RDSIIL FQ+AKI+VLEFDDSTHSL+TSSMHCF+GP+WLHLKRGRESFARG
Sbjct: 121  IGGGDVSRRRDSIILTFQDAKIAVLEFDDSTHSLQTSSMHCFEGPEWLHLKRGRESFARG 180

Query: 181  PVVKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLINLRD 240
            P+VK DPQGRC GVLVYGLQMIILKA+QAGSG V +D+AFG+   +SARVESSY+INLRD
Sbjct: 181  PLVKADPQGRCSGVLVYGLQMIILKAAQAGSGFVGEDDAFGSGATVSARVESSYIINLRD 240

Query: 241  LDVKHVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALSISTTLKQHPLIWS 300
            LD+KH+KDF+FVHGYIEPVMVILHE+ELTWAGRVSWKHHTCM+SALSISTTLKQHPLIWS
Sbjct: 241  LDMKHIKDFIFVHGYIEPVMVILHERELTWAGRVSWKHHTCMISALSISTTLKQHPLIWS 300

Query: 301  ASNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCMLALNNYAVSADSSQDMPRSNF 360
            A+NLPHDAYKLLAVPSPIGGVLVISAN IHY+SQSA+C LALNNYA S D+SQ++PRS+F
Sbjct: 301  AANLPHDAYKLLAVPSPIGGVLVISANMIHYHSQSATCALALNNYAASVDNSQELPRSSF 360

Query: 361  NVELDAANATWLVNDVALLSTKTGELLLLALVYDGRVVQRLDLSKSKASVLTSGIASIGN 420
            NVELDAANATWL+NDVALLS KTGELLLL LVYDGRVVQRLDLSKSKASVLTS I +IGN
Sbjct: 361  NVELDAANATWLLNDVALLSAKTGELLLLTLVYDGRVVQRLDLSKSKASVLTSDITTIGN 420

Query: 421  SLFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMRRSSSDALQDMVG 480
            SL FLGSRLGDSLLVQFS G G+S L S LK+E GDIE D   AKR+RRSSSDALQD VG
Sbjct: 421  SLVFLGSRLGDSLLVQFSSGSGASTLPSGLKEEVGDIEGDVPLAKRLRRSSSDALQDAVG 480

Query: 481  GDELSLYGSAANNTESAQKIFSFAVRDSLINIGPLKDFSYGLRINADPNATGIAKQSNYE 540
             +ELSLYGS  NN+ESAQK F FAVRDSLIN+GPLKDFSYGLRINAD NATGIAKQSNYE
Sbjct: 481  SEELSLYGSTPNNSESAQKAFLFAVRDSLINVGPLKDFSYGLRINADANATGIAKQSNYE 540

Query: 541  LVCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVYHKNTRGSIADSSRTVPDDDE 600
            LVCCSGHGKNGALC+LRQSIRPEMITEVEL GCKGIWTVYHK+TRG  ADSS+   DDDE
Sbjct: 541  LVCCSGHGKNGALCVLRQSIRPEMITEVELTGCKGIWTVYHKSTRGHNADSSKLADDDDE 600

Query: 601  YHAYLIISLEARTMVLVTGELLTEVTESVDYFVHGRTIAAGNLFGRRRVIQVYESGARIL 660
            YHAYLIISLEARTMVL T +LLTEVTESVDY+V GRTIAAGNLFGRRRVIQV+E GARIL
Sbjct: 601  YHAYLIISLEARTMVLETADLLTEVTESVDYYVQGRTIAAGNLFGRRRVIQVFERGARIL 660

Query: 661  DGSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMTDGSIRLLVGDSSSCSVSV 720
            DGSFMTQ+L++ +  +E+ + S+  TV+S SI+DPYVLL MTDGSI LLVGD ++C+VS+
Sbjct: 661  DGSFMTQELSIPLPNSETSSGSDNSTVMSVSIADPYVLLRMTDGSILLLVGDPATCTVSI 720

Query: 721  SAPAAFGSSKKCVSSCTLYQDKGFEPWLRMTSTDAWLSTGVGETIDGTDGSLQDQGDIYC 780
            ++PAAF  SKK VS+C+LY DKG EPWLR  S+DAWLSTG+GE+ID  DG   DQGDIYC
Sbjct: 721  NSPAAFEGSKKRVSACSLYHDKGPEPWLRKASSDAWLSTGIGESIDSADGGPHDQGDIYC 780

Query: 781  VACYDNGDLEIFDVPNFTSVFYVDKFVSGKSHLVDHQISDLQKSSE--VDQNSQELISHG 840
            V CY+NG LEIFDVPNF  VF V+KF SG++HLVD    +  + SE  ++++S+EL    
Sbjct: 781  VICYENGALEIFDVPNFNCVFSVEKFASGRAHLVDAYSQESSEGSEKPINKSSEELAGQS 840

Query: 841  RNESSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFESTDSASKIDDSVSID 900
            R E+  N+KV+E+AMQRWSG HSRPF+FGILTDGTILCYHAYLFE  D+ASK++ S S  
Sbjct: 841  RKENVHNLKVVELAMQRWSGNHSRPFIFGILTDGTILCYHAYLFEGPDNASKVEGSASAQ 900

Query: 901  NSVSSSNMSSSRLRNLRFLRVPLDIQGREDMPNGTLSRRLSIFKNISGYQGLFLCGSRPA 960
            NSV  SN+++SRLRNLRF+RV LD   RE+  NGTLS+R++IFKNISGYQG FL G RPA
Sbjct: 901  NSVGLSNVNASRLRNLRFIRVSLDAYTREETSNGTLSQRITIFKNISGYQGFFLSGLRPA 960

Query: 961  WFMVFRERLRVHPQLCDGPIVAFAVLHNVNCNHGLIYVTSQ------------------- 1020
            WFMVFR+RLR+HPQ+CDG IVAF VLHNVNCNHG IYVTSQ                   
Sbjct: 961  WFMVFRQRLRIHPQICDGSIVAFTVLHNVNCNHGFIYVTSQGILKICQMPSTSNYDNYWP 1020

Query: 1021 ---VPLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQVLSSMVDQDVGH-VENHNLSADEL 1080
               +PL+GTPHQVTYF E+NLYP+I+S PV KP+NQVLSS+VDQ+ GH ++N NLS+DEL
Sbjct: 1021 VQKIPLRGTPHQVTYFAERNLYPLIVSVPVHKPVNQVLSSLVDQEAGHQMDNLNLSSDEL 1080

Query: 1081 QQTYSVEEFEIRILEPEKSGGPWQTRATIAMHSSENALTIRVVTLLNTTTKENETLLAVG 1140
             +TY+VEEFE+RILEPEKSGGPW+T+ATI M SSENALT+RVVTL NTTTKENETLLA+G
Sbjct: 1081 HRTYTVEEFEVRILEPEKSGGPWETKATIPMQSSENALTVRVVTLFNTTTKENETLLAIG 1140

Query: 1141 TAYVQGEDVAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALASLQGHLLIASGPK 1200
            TAYVQGEDVAARGRVLLFS+G+  DN+Q LVSEVYSKELKGAISALASLQGHLLIASGPK
Sbjct: 1141 TAYVQGEDVAARGRVLLFSIGRSTDNNQNLVSEVYSKELKGAISALASLQGHLLIASGPK 1200

Query: 1201 IILHKWTGAELNGIAFYDVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLLAK 1260
            IILH WTG+ELNGIAFYD PPLYVVSLNIVKNFILLGD+HKSIYFLSWKEQGAQLSLLAK
Sbjct: 1201 IILHIWTGSELNGIAFYDAPPLYVVSLNIVKNFILLGDVHKSIYFLSWKEQGAQLSLLAK 1260

Query: 1261 DFGSLDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTESWKGQKLLSRAEFHVGAHV 1320
            DFGSLDC+ATEFLIDGSTLSL VSDDQKNIQ+FYYAPK +ESW+GQKLLSRAEFHVGA V
Sbjct: 1261 DFGSLDCFATEFLIDGSTLSLMVSDDQKNIQVFYYAPKMSESWRGQKLLSRAEFHVGARV 1320

Query: 1321 TKFLRLQMLSTSSDKACSTVSDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKLG 1380
            TKFLRLQMLSTS   + +   DKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKL 
Sbjct: 1321 TKFLRLQMLSTSGRTSATAGPDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKLV 1380

Query: 1381 DAVPHVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELLCHYEMLPLEEQLDIAHQIGTTRS 1431
            DAVPHV GLNPRSFR F SNGK HR GPDSIVDCELLCHYEMLPLEEQL+IAHQIGTTRS
Sbjct: 1381 DAVPHVAGLNPRSFRHFRSNGKAHRPGPDSIVDCELLCHYEMLPLEEQLEIAHQIGTTRS 1440

BLAST of Cucsa.178510 vs. TrEMBL
Match: V4S829_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10004147mg PE=4 SV=1)

HSP 1 Score: 2266.5 bits (5872), Expect = 0.0e+00
Identity = 1133/1457 (77.76%), Postives = 1268/1457 (87.03%), Query Frame = 1

Query: 1    MSFAAYRMMHWPTGIENCDSAYITHSRADFVPAVTS-HSDDLDSDWHPRRDIGPVPNLVV 60
            MSFAAY+MMHWPTGI NC S +ITHSRAD+VP +    +++LDS+   +R IGPVPNLVV
Sbjct: 1    MSFAAYKMMHWPTGIANCGSGFITHSRADYVPQIPLIQTEELDSELPSKRGIGPVPNLVV 60

Query: 61   TAGNVLEVYVVRVLEEGGRESKSSGEVRRGGIMDGVSGASLELVCHYRLHGNVESMAILS 120
            TA NV+E+YVVRV EEG +ESK+SGE +R  +MDG+S ASLELVCHYRLHGNVES+AILS
Sbjct: 61   TAANVIEIYVVRVQEEGSKESKNSGETKRRVLMDGISAASLELVCHYRLHGNVESLAILS 120

Query: 121  SRGGDGSKKRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWLHLKRGRESFARG 180
              G D S++RDSIIL F++AKISVLEFDDS H LR +SMHCF+ P+WLHLKRGRESFARG
Sbjct: 121  QGGADNSRRRDSIILAFEDAKISVLEFDDSIHGLRITSMHCFESPEWLHLKRGRESFARG 180

Query: 181  PVVKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLINLRD 240
            P+VKVDPQGRCGGVLVYGLQMIILKASQ GSGLV D++ FG+ G  SAR+ESS++INLRD
Sbjct: 181  PLVKVDPQGRCGGVLVYGLQMIILKASQGGSGLVGDEDTFGSGGGFSARIESSHVINLRD 240

Query: 241  LDVKHVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALSISTTLKQHPLIWS 300
            LD+KHVKDF+FVHGYIEPVMVILHE+ELTWAGRVSWKHHTCM+SALSISTTLKQHPLIWS
Sbjct: 241  LDMKHVKDFIFVHGYIEPVMVILHERELTWAGRVSWKHHTCMISALSISTTLKQHPLIWS 300

Query: 301  ASNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCMLALNNYAVSADSSQDMPRSNF 360
            A NLPHDAYKLLAVPSPIGGVLV+ AN+IHY+SQSASC LALNNYAVS DSSQ++PRS+F
Sbjct: 301  AMNLPHDAYKLLAVPSPIGGVLVVGANTIHYHSQSASCALALNNYAVSLDSSQELPRSSF 360

Query: 361  NVELDAANATWLVNDVALLSTKTGELLLLALVYDGRVVQRLDLSKSKASVLTSGIASIGN 420
            +VELDAA+ATWL NDVALLSTKTG+L+LL +VYDGRVVQRLDLSK+  SVLTS I +IGN
Sbjct: 361  SVELDAAHATWLQNDVALLSTKTGDLVLLTVVYDGRVVQRLDLSKTNPSVLTSDITTIGN 420

Query: 421  SLFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMRRSSSDALQDMVG 480
            SLFFLGSRLGDSLLVQF+CG G+S L+S  K+E GDIE DA + KR+RRSSSDALQDMV 
Sbjct: 421  SLFFLGSRLGDSLLVQFTCGSGTSMLSSGPKEEFGDIEADAPSTKRLRRSSSDALQDMVN 480

Query: 481  GDELSLYGSAANNTESAQKIFSFAVRDSLINIGPLKDFSYGLRINADPNATGIAKQSNYE 540
            G+ELSLYGSA+NNTESAQK FSFAVRDSL+NIGPLKDFSYGLRINAD +ATGI+KQSNYE
Sbjct: 481  GEELSLYGSASNNTESAQKTFSFAVRDSLVNIGPLKDFSYGLRINADASATGISKQSNYE 540

Query: 541  LVCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVYHKNTRGSIADSSRTVPDDDE 600
            LVCCSGHGKNGALC+LRQSIRPEMITEVELPGCKGIWTVYHK++RG   DSSR    DDE
Sbjct: 541  LVCCSGHGKNGALCVLRQSIRPEMITEVELPGCKGIWTVYHKSSRGHNTDSSRMAAYDDE 600

Query: 601  YHAYLIISLEARTMVLVTGELLTEVTESVDYFVHGRTIAAGNLFGRRRVIQVYESGARIL 660
            YHAYLIISLEARTMVL T +LLTEVTESVDYFV GRTIAAGNLFGRRRVIQV+E GARIL
Sbjct: 601  YHAYLIISLEARTMVLETADLLTEVTESVDYFVQGRTIAAGNLFGRRRVIQVFERGARIL 660

Query: 661  DGSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMTDGSIRLLVGDSSSCSVSV 720
            DGS+MTQDL+   + +ESG+ SE  TVLS SI+DPYVLL M+DGSIRLLVGD S+C+VSV
Sbjct: 661  DGSYMTQDLSFGPSNSESGSGSENSTVLSVSIADPYVLLGMSDGSIRLLVGDPSTCTVSV 720

Query: 721  SAPAAFGSSKKCVSSCTLYQDKGFEPWLRMTSTDAWLSTGVGETIDGTDGSLQDQGDIYC 780
              PAA  SSKK VS+CTLY DKG EPWLR TSTDAWLSTGVGE IDG DG   DQGDIY 
Sbjct: 721  QTPAAIESSKKPVSACTLYHDKGPEPWLRKTSTDAWLSTGVGEAIDGADGGPLDQGDIYS 780

Query: 781  VACYDNGDLEIFDVPNFTSVFYVDKFVSGKSHLVDHQISDLQKSS--EVDQNSQELISHG 840
            V CY++G LEIFDVPNF  VF VDKFVSG++H+VD  + +  K S  E++ +S+E    G
Sbjct: 781  VVCYESGALEIFDVPNFNCVFTVDKFVSGRTHIVDTYMREALKDSETEINSSSEEGTGQG 840

Query: 841  RNESSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFESTDSASKIDDSVSID 900
            R E+  +MKV+E+AMQRWSG HSRPFLF ILTDGTILCY AYLFE +++ SK DD VS  
Sbjct: 841  RKENIHSMKVVELAMQRWSGHHSRPFLFAILTDGTILCYQAYLFEGSENTSKSDDPVSTS 900

Query: 901  NSVSSSNMSSSRLRNLRFLRVPLDIQGREDMPNGTLSRRLSIFKNISGYQGLFLCGSRPA 960
             S+S SN+S+SRLRNLRF R PLD   RE+ P+G   +R++IFKNISG+QG FL GSRP 
Sbjct: 901  RSLSVSNVSASRLRNLRFSRTPLDAYTREETPHGAPCQRITIFKNISGHQGFFLSGSRPC 960

Query: 961  WFMVFRERLRVHPQLCDGPIVAFAVLHNVNCNHGLIYVTSQ------------------- 1020
            W MVFRERLRVHPQLCDG IVAF VLHNVNCNHG IYVTSQ                   
Sbjct: 961  WCMVFRERLRVHPQLCDGSIVAFTVLHNVNCNHGFIYVTSQGILKICQLPSGSTYDNYWP 1020

Query: 1021 ---VPLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQVLSSMVDQDVGH-VENHNLSADEL 1080
               +PLK TPHQ+TYF EKNLYP+I+S PV KPLNQVLS ++DQ+VGH ++NHNLS+ +L
Sbjct: 1021 VQKIPLKATPHQITYFAEKNLYPLIVSVPVLKPLNQVLSLLIDQEVGHQIDNHNLSSVDL 1080

Query: 1081 QQTYSVEEFEIRILEPEKSGGPWQTRATIAMHSSENALTIRVVTLLNTTTKENETLLAVG 1140
             +TY+VEE+E+RILEP+++GGPWQTRATI M SSENALT+RVVTL NTTTKEN+TLLA+G
Sbjct: 1081 HRTYTVEEYEVRILEPDRAGGPWQTRATIPMQSSENALTVRVVTLFNTTTKENDTLLAIG 1140

Query: 1141 TAYVQGEDVAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALASLQGHLLIASGPK 1200
            TAYVQGEDVAARGRVLLFS G++ADN Q LV+EVYSKELKGAISALASLQGHLLIASGPK
Sbjct: 1141 TAYVQGEDVAARGRVLLFSTGRNADNPQNLVTEVYSKELKGAISALASLQGHLLIASGPK 1200

Query: 1201 IILHKWTGAELNGIAFYDVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLLAK 1260
            IILHKWTG ELNGIAFYD PPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQL+LLAK
Sbjct: 1201 IILHKWTGTELNGIAFYDAPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLNLLAK 1260

Query: 1261 DFGSLDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTESWKGQKLLSRAEFHVGAHV 1320
            DFGSLDC+ATEFLIDGSTLSL VSD+QKNIQIFYYAPK +ESWKGQKLLSRAEFHVGAHV
Sbjct: 1261 DFGSLDCFATEFLIDGSTLSLVVSDEQKNIQIFYYAPKMSESWKGQKLLSRAEFHVGAHV 1320

Query: 1321 TKFLRLQMLSTSSDK-ACSTVSDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKL 1380
            TKFLRLQML+TSSD+   +  SDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKL
Sbjct: 1321 TKFLRLQMLATSSDRTGAAPGSDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKL 1380

Query: 1381 GDAVPHVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELLCHYEMLPLEEQLDIAHQIGTTR 1431
             D+VPHV GLNPRSFRQFHSNGK HR GPDSIVDCELL HYEMLPLEEQL+IAHQ GTTR
Sbjct: 1381 VDSVPHVAGLNPRSFRQFHSNGKAHRPGPDSIVDCELLSHYEMLPLEEQLEIAHQTGTTR 1440

BLAST of Cucsa.178510 vs. TAIR10
Match: AT5G51660.1 (AT5G51660.1 cleavage and polyadenylation specificity factor 160)

HSP 1 Score: 2099.7 bits (5439), Expect = 0.0e+00
Identity = 1042/1458 (71.47%), Postives = 1224/1458 (83.95%), Query Frame = 1

Query: 1    MSFAAYRMMHWPTGIENCDSAYITHSRADF---VPAVTSHSDDLDSDW-HPRRDIGPVPN 60
            MSFAAY+MMHWPTG+ENC S YITHS +D    +P V+ H DD++++W +P+R IGP+PN
Sbjct: 1    MSFAAYKMMHWPTGVENCASGYITHSLSDSTLQIPIVSVH-DDIEAEWPNPKRGIGPLPN 60

Query: 61   LVVTAGNVLEVYVVRVLEEGG-RESKSSGEVRRGGIMDGVSGASLELVCHYRLHGNVESM 120
            +V+TA N+LEVY+VR  EEG  +E ++    +RGG+MDGV G SLELVCHYRLHGNVES+
Sbjct: 61   VVITAANILEVYIVRAQEEGNTQELRNPKLAKRGGVMDGVYGVSLELVCHYRLHGNVESI 120

Query: 121  AILSSRGGDGSKKRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWLHLKRGRES 180
            A+L   GG+ SK RDSIIL F++AKISVLEFDDS HSLR +SMHCF+GP WLHLKRGRES
Sbjct: 121  AVLPMGGGNSSKGRDSIILTFRDAKISVLEFDDSIHSLRMTSMHCFEGPDWLHLKRGRES 180

Query: 181  FARGPVVKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLI 240
            F RGP+VKVDPQGRCGGVLVYGLQMIILK SQ GSGLV DD+AF + G +SARVESSY+I
Sbjct: 181  FPRGPLVKVDPQGRCGGVLVYGLQMIILKTSQVGSGLVGDDDAFSSGGTVSARVESSYII 240

Query: 241  NLRDLDVKHVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALSISTTLKQHP 300
            NLRDL++KHVKDFVF+HGYIEPV+VIL E+E TWAGRVSWKHHTC++SALSI++TLKQHP
Sbjct: 241  NLRDLEMKHVKDFVFLHGYIEPVIVILQEEEHTWAGRVSWKHHTCVLSALSINSTLKQHP 300

Query: 301  LIWSASNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCMLALNNYAVSADSSQDMP 360
            +IWSA NLPHDAYKLLAVPSPIGGVLV+ AN+IHY+SQSASC LALNNYA SADSSQ++P
Sbjct: 301  VIWSAINLPHDAYKLLAVPSPIGGVLVLCANTIHYHSQSASCALALNNYASSADSSQELP 360

Query: 361  RSNFNVELDAANATWLVNDVALLSTKTGELLLLALVYDGRVVQRLDLSKSKASVLTSGIA 420
             SNF+VELDAA+ TW+ NDVALLSTK+GELLLL L+YDGR VQRLDLSKSKASVL S I 
Sbjct: 361  ASNFSVELDAAHGTWISNDVALLSTKSGELLLLTLIYDGRAVQRLDLSKSKASVLASDIT 420

Query: 421  SIGNSLFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMRRSSSDALQ 480
            S+GNSLFFLGSRLGDSLLVQFSC  G +     L+DE  DIE + H AKR+ R +SD  Q
Sbjct: 421  SVGNSLFFLGSRLGDSLLVQFSCRSGPAASLPGLRDEDEDIEGEGHQAKRL-RMTSDTFQ 480

Query: 481  DMVGGDELSLYGSAANNTESAQKIFSFAVRDSLINIGPLKDFSYGLRINADPNATGIAKQ 540
            D +G +ELSL+GS  NN++SAQK FSFAVRDSL+N+GP+KDF+YGLRINAD NATG++KQ
Sbjct: 481  DTIGNEELSLFGSTPNNSDSAQKSFSFAVRDSLVNVGPVKDFAYGLRINADANATGVSKQ 540

Query: 541  SNYELVCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVYHKNTRGSIADSSRTVP 600
            SNYELVCCSGHGKNGALC+LRQSIRPEMITEVELPGCKGIWTVYHK++RG  ADSS+   
Sbjct: 541  SNYELVCCSGHGKNGALCVLRQSIRPEMITEVELPGCKGIWTVYHKSSRGHNADSSKMAA 600

Query: 601  DDDEYHAYLIISLEARTMVLVTGELLTEVTESVDYFVHGRTIAAGNLFGRRRVIQVYESG 660
            D+DEYHAYLIISLEARTMVL T +LLTEVTESVDY+V GRTIAAGNLFGRRRVIQV+E G
Sbjct: 601  DEDEYHAYLIISLEARTMVLETADLLTEVTESVDYYVQGRTIAAGNLFGRRRVIQVFEHG 660

Query: 661  ARILDGSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMTDGSIRLLVGDSSSC 720
            ARILDGSFM Q+L+   + +ES + SE  TV S SI+DPYVLL MTD SIRLLVGD S+C
Sbjct: 661  ARILDGSFMNQELSFGASNSESNSGSESSTVSSVSIADPYVLLRMTDDSIRLLVGDPSTC 720

Query: 721  SVSVSAPAAFGSSKKCVSSCTLYQDKGFEPWLRMTSTDAWLSTGVGETIDGTDGSLQDQG 780
            +VS+S+P+    SK+ +S+CTLY DKG EPWLR  STDAWLS+GVGE +D  DG  QDQG
Sbjct: 721  TVSISSPSVLEGSKRKISACTLYHDKGPEPWLRKASTDAWLSSGVGEAVDSVDGGPQDQG 780

Query: 781  DIYCVACYDNGDLEIFDVPNFTSVFYVDKFVSGKSHLVDHQISDLQKSSEVDQNSQELIS 840
            DIYCV CY++G LEIFDVP+F  VF VDKF SG+ HL D  I +L+   E+++NS++  S
Sbjct: 781  DIYCVVCYESGALEIFDVPSFNCVFSVDKFASGRRHLSDMPIHELE--YELNKNSEDNTS 840

Query: 841  HGRNESSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFESTDSASKIDDSVS 900
               ++  +N +V+E+AMQRWSG H+RPFLF +L DGTILCYHAYLF+  DS +K ++S+S
Sbjct: 841  ---SKEIKNTRVVELAMQRWSGHHTRPFLFAVLADGTILCYHAYLFDGVDS-TKAENSLS 900

Query: 901  IDNSVSSSNMSSSRLRNLRFLRVPLDIQGREDMPNGTLSRRLSIFKNISGYQGLFLCGSR 960
             +N  + ++  SS+LRNL+FLR+PLD   RE   +G  S+R+++FKNISG+QG FL GSR
Sbjct: 901  SENPAALNSSGSSKLRNLKFLRIPLDTSTREGTSDGVASQRITMFKNISGHQGFFLSGSR 960

Query: 961  PAWFMVFRERLRVHPQLCDGPIVAFAVLHNVNCNHGLIYVTSQ----------------- 1020
            P W M+FRERLR H QLCDG I AF VLHNVNCNHG IYVT+Q                 
Sbjct: 961  PGWCMLFRERLRFHSQLCDGSIAAFTVLHNVNCNHGFIYVTAQGVLKICQLPSASIYDNY 1020

Query: 1021 -----VPLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQVLSSMVDQDVG-HVENHNLSAD 1080
                 +PLK TPHQVTY+ EKNLYP+I+S PV KPLNQVLSS+VDQ+ G  ++NHN+S+D
Sbjct: 1021 WPVQKIPLKATPHQVTYYAEKNLYPLIVSYPVSKPLNQVLSSLVDQEAGQQLDNHNMSSD 1080

Query: 1081 ELQQTYSVEEFEIRILEPEKSGGPWQTRATIAMHSSENALTIRVVTLLNTTTKENETLLA 1140
            +LQ+TY+VEEFEI+ILEPE+SGGPW+T+A I M +SE+ALT+RVVTLLN +T ENETLLA
Sbjct: 1081 DLQRTYTVEEFEIQILEPERSGGPWETKAKIPMQTSEHALTVRVVTLLNASTGENETLLA 1140

Query: 1141 VGTAYVQGEDVAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALASLQGHLLIASG 1200
            VGTAYVQGEDVAARGRVLLFS GK+ DNSQ +V+EVYS+ELKGAISA+AS+QGHLLI+SG
Sbjct: 1141 VGTAYVQGEDVAARGRVLLFSFGKNGDNSQNVVTEVYSRELKGAISAVASIQGHLLISSG 1200

Query: 1201 PKIILHKWTGAELNGIAFYDVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLL 1260
            PKIILHKW G ELNG+AF+D PPLYVVS+N+VK+FILLGD+HKSIYFLSWKEQG+QLSLL
Sbjct: 1201 PKIILHKWNGTELNGVAFFDAPPLYVVSMNVVKSFILLGDVHKSIYFLSWKEQGSQLSLL 1260

Query: 1261 AKDFGSLDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTESWKGQKLLSRAEFHVGA 1320
            AKDF SLDC+ATEFLIDGSTLSL VSD+QKNIQ+FYYAPK  ESWKG KLLSRAEFHVGA
Sbjct: 1261 AKDFESLDCFATEFLIDGSTLSLAVSDEQKNIQVFYYAPKMIESWKGLKLLSRAEFHVGA 1320

Query: 1321 HVTKFLRLQMLSTSSDKACSTVSDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKK 1380
            HV+KFLRLQM+S+         +DK NRFALLFGTLDGS GCIAPLDE+TFRRLQSLQKK
Sbjct: 1321 HVSKFLRLQMVSSG--------ADKINRFALLFGTLDGSFGCIAPLDEVTFRRLQSLQKK 1380

Query: 1381 LGDAVPHVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELLCHYEMLPLEEQLDIAHQIGTT 1431
            L DAVPHV GLNP +FRQF S+GK  R GPDSIVDCELLCHYEMLPLEEQL++AHQIGTT
Sbjct: 1381 LVDAVPHVAGLNPLAFRQFRSSGKARRSGPDSIVDCELLCHYEMLPLEEQLELAHQIGTT 1440

BLAST of Cucsa.178510 vs. TAIR10
Match: AT4G05420.1 (AT4G05420.1 damaged DNA binding protein 1A)

HSP 1 Score: 92.0 bits (227), Expect = 3.1e-18
Identity = 87/329 (26.44%), Postives = 152/329 (46.20%), Query Frame = 1

Query: 1104 TKENETLLAVGTAYVQGED-VAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALAS 1163
            T++      VGTAYV  E+    +GR+L+F V    D    L++E   KE KGA+ +L +
Sbjct: 777  TEDKNVYYCVGTAYVLPEENEPTKGRILVFIV---EDGRLQLIAE---KETKGAVYSLNA 836

Query: 1164 LQGHLLIASGPKIILHKWT-----GAELNGIAFY--DVPPLYVVSLNIVKNFILLGDIHK 1223
              G LL A   KI L+KW        EL     +   +  LYV +     +FI++GD+ K
Sbjct: 837  FNGKLLAAINQKIQLYKWMLRDDGTRELQSECGHHGHILALYVQTRG---DFIVVGDLMK 896

Query: 1224 SIYFLSWKEQGAQLSLLAKDFGSLDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTE 1283
            SI  L +K +   +   A+D+ +    A E L D   + L   ++   + +   +  +T+
Sbjct: 897  SISLLLYKHEEGAIEERARDYNANWMSAVEILDD--DIYLGAENNFNLLTVKKNSEGATD 956

Query: 1284 SWKGQKLLSRAEFHVGAHVTKFLRLQMLSTSSDKACSTVSDKTNRFALLFGTLDGSIGCI 1343
              +G +L    E+H+G  V +F    ++    D     +        ++FGT++G IG I
Sbjct: 957  EERG-RLEVVGEYHLGEFVNRFRHGSLVMRLPDSEIGQIP------TVIFGTVNGVIGVI 1016

Query: 1344 APLDELTFRRLQSLQKKLGDAVPHVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELLCHYE 1403
            A L +  +  L+ LQ  L   +  VGGL+   +R F  N +       + +D +L+  + 
Sbjct: 1017 ASLPQEQYTFLEKLQSSLRKVIKGVGGLSHEQWRSF--NNEKRTAEARNFLDGDLIESFL 1076

Query: 1404 MLPLEEQLDIAHQIGTTRSQILSNLNDLS 1425
             L   +  DI+  +     ++   + +L+
Sbjct: 1077 DLSRNKMEDISKSMNVQVEELCKRVEELT 1085

HSP 2 Score: 60.8 bits (146), Expect = 7.7e-09
Identity = 76/341 (22.29%), Postives = 140/341 (41.06%), Query Frame = 1

Query: 108 LHGNVESMAILSSRGGDGSKKRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWL 167
           ++G + ++ +    G    + +D + +  +  K  VL++D  +  L T +M         
Sbjct: 58  IYGRIATLELFRPHG----EAQDFLFIATERYKFCVLQWDPESSELITRAMGDVSD---- 117

Query: 168 HLKRGRESFARGPVVKVDPQGRCGGVLVY-GLQMIILKASQAGSGLVVDDEAFGNTGAIS 227
             + GR +   G +  +DP  R  G+ +Y GL  +I                F N G   
Sbjct: 118 --RIGRPT-DNGQIGIIDPDCRLIGLHLYDGLFKVI---------------PFDNKG--- 177

Query: 228 ARVESSYLINLRDLDVKHVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALS 287
            +++ ++ I L +L V  +K   F+ G  +P + +L++     A  V            +
Sbjct: 178 -QLKEAFNIRLEELQVLDIK---FLFGCAKPTIAVLYQDNKD-ARHVK-----------T 237

Query: 288 ISTTLKQHPLI---WSASNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCMLALNN 347
              +LK    +   WS ++L + A  L+ VP P+ GVL+I   +I Y S SA   + +  
Sbjct: 238 YEVSLKDKDFVEGPWSQNSLDNGADLLIPVPPPLCGVLIIGEETIVYCSASAFKAIPIRP 297

Query: 348 YAVSADSSQDMPRSNFNVELDAANATWLVNDVALLSTKTGELLLLALVYDGRVVQRLDLS 407
               A    D+  S +                 LL    G + LL + ++   V  L + 
Sbjct: 298 SITKAYGRVDVDGSRY-----------------LLGDHAGMIHLLVITHEKEKVTGLKIE 336

Query: 408 KSKASVLTSGIASIGNSLFFLGSRLGDSLLVQFSCGVGSSG 445
               + + S I+ + N++ F+GS  GDS LV+ +    + G
Sbjct: 358 LLGETSIASTISYLDNAVVFVGSSYGDSQLVKLNLHPDAKG 336

HSP 3 Score: 52.0 bits (123), Expect = 3.6e-06
Identity = 55/216 (25.46%), Postives = 95/216 (43.98%), Query Frame = 1

Query: 504 VRDSLINIGPLKDFSYGLRINADPNATGIAKQSNYELVCCSGHGKNGALCILRQSIRPEM 563
           V +  IN+GP+ DF              + +Q   ++V CSG  K+G+L ++R  I    
Sbjct: 341 VLERYINLGPIVDFC----------VVDLERQGQGQVVTCSGAFKDGSLRVVRNGIGINE 400

Query: 564 ITEVELPGCKGIWTVYHKNTRGSIADSSRTVPDDDEYHAYLIISLEARTMVLVTG-ELLT 623
              VEL G KG+W++     + SI         D+ +  +L++S  + T +L    E   
Sbjct: 401 QASVELQGIKGMWSL-----KSSI---------DEAFDTFLVVSFISETRILAMNLEDEL 460

Query: 624 EVTESVDYFVHGRTIAAGNLFGRRRVIQVYESGARILDGSFMTQDLNLVVNGNESGNASE 683
           E TE   +    +T+   +     +++QV  +  R++  S  T++L       +  +A  
Sbjct: 461 EETEIEGFLSQVQTLFCHDAV-YNQLVQVTSNSVRLV--SSTTREL------RDEWHAPA 520

Query: 684 GCTVLSASISDPYVLLTMTDGS-IRLLVGDSSSCSV 718
           G TV  A+ +   VLL    G  + L +GD     V
Sbjct: 521 GFTVNVATANASQVLLATGGGHLVYLEIGDGKLTEV 523

BLAST of Cucsa.178510 vs. TAIR10
Match: AT4G21100.1 (AT4G21100.1 damaged DNA binding protein 1B)

HSP 1 Score: 84.7 bits (208), Expect = 5.0e-16
Identity = 80/278 (28.78%), Postives = 127/278 (45.68%), Query Frame = 1

Query: 1104 TKENETLLAVGTAYVQGED-VAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALAS 1163
            T +      VGTAYV  E+    +GR+L+F V    +    L++E   KE KGA+ +L +
Sbjct: 777  TDDKNVYYCVGTAYVLPEENEPTKGRILVFIV---EEGRLQLITE---KETKGAVYSLNA 836

Query: 1164 LQGHLLIASGPKIILHKWT-----GAELNGIAFY--DVPPLYVVSLNIVKNFILLGDIHK 1223
              G LL +   KI L+KW        EL     +   +  LYV +     +FI +GD+ K
Sbjct: 837  FNGKLLASINQKIQLYKWMLRDDGTRELQSECGHHGHILALYVQTRG---DFIAVGDLMK 896

Query: 1224 SIYFLSWKEQGAQLSLLAKDFGSLDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTE 1283
            SI  L +K +   +   A+D+ +    A E L D   L    +D+  NI       +   
Sbjct: 897  SISLLIYKHEEGAIEERARDYNANWMTAVEILNDDIYLG---TDNCFNIFTVKKNNEGAT 956

Query: 1284 SWKGQKLLSRAEFHVGAHVTKFLRLQMLSTSSDKACSTVSDKTNRFALLFGTLDGSIGCI 1343
              +  ++    E+H+G  V +F    ++    D      SD      ++FGT+ G IG I
Sbjct: 957  DEERARMEVVGEYHIGEFVNRFRHGSLVMKLPD------SDIGQIPTVIFGTVSGMIGVI 1016

Query: 1344 APLDELTFRRLQSLQKKLGDAVPHVGGLNPRSFRQFHS 1374
            A L +  +  L+ LQ  L   +  VGGL+   +R F++
Sbjct: 1017 ASLPQEQYAFLEKLQTSLRKVIKGVGGLSHEQWRSFNN 1036

HSP 2 Score: 67.4 bits (163), Expect = 8.2e-11
Identity = 80/354 (22.60%), Postives = 147/354 (41.53%), Query Frame = 1

Query: 95  VSGASLELVCHYRLHGNVESMAILSSRGGDGSKKRDSIILVFQEAKISVLEFDDSTHSLR 154
           +S   L+ +    L+G + +M +    G    + +D + +  +  K  VL++D  +  L 
Sbjct: 45  LSPQGLQTILDVPLYGRIATMELFRPHG----EAQDFLFVATERYKFCVLQWDYESSELI 104

Query: 155 TSSMHCFDGPQWLHLKRGRESFARGPVVKVDPQGRCGGVLVY-GLQMIILKASQAGSGLV 214
           T +M           + GR +   G +  +DP  R  G+ +Y GL  +I           
Sbjct: 105 TRAMGDVSD------RIGRPT-DNGQIGIIDPDCRVIGLHLYDGLFKVI----------- 164

Query: 215 VDDEAFGNTGAISARVESSYLINLRDLDVKHVKDFVFVHGYIEPVMVILHEQELTWAGRV 274
                F N G    +++ ++ I L +L V  +K   F++G  +P + +L++     A  V
Sbjct: 165 ----PFDNKG----QLKEAFNIRLEELQVLDIK---FLYGCTKPTIAVLYQDNKD-ARHV 224

Query: 275 SWKHHTCMVSALSISTTLKQHPLI---WSASNLPHDAYKLLAVPSPIGGVLVISANSIHY 334
                       +   +LK    +   WS +NL + A  L+ VPSP+ GVL+I   +I Y
Sbjct: 225 K-----------TYEVSLKDKNFVEGPWSQNNLDNGADLLIPVPSPLCGVLIIGEETIVY 284

Query: 335 NSQSASCMLALNNYAVSADSSQDMPRSNFNVELDAANATWLVNDVALLSTKTGELLLLAL 394
            S +A   + +      A    D+  S +                 LL    G + LL +
Sbjct: 285 CSANAFKAIPIRPSITKAYGRVDLDGSRY-----------------LLGDHAGLIHLLVI 336

Query: 395 VYDGRVVQRLDLSKSKASVLTSGIASIGNSLFFLGSRLGDSLLVQFSCGVGSSG 445
            ++   V  L +     + + S I+ + N++ F+GS  GDS L++ +    + G
Sbjct: 345 THEKEKVTGLKIELLGETSIASSISYLDNAVVFVGSSYGDSQLIKLNLQPDAKG 336

HSP 3 Score: 50.1 bits (118), Expect = 1.4e-05
Identity = 53/216 (24.54%), Postives = 95/216 (43.98%), Query Frame = 1

Query: 504 VRDSLINIGPLKDFSYGLRINADPNATGIAKQSNYELVCCSGHGKNGALCILRQSIRPEM 563
           + +  +N+GP+ DF              + +Q   ++V CSG  K+G+L I+R  I    
Sbjct: 341 ILEKYVNLGPIVDFC----------VVDLERQGQGQVVTCSGAYKDGSLRIVRNGIGINE 400

Query: 564 ITEVELPGCKGIWTVYHKNTRGSIADSSRTVPDDDEYHAYLIISLEARTMVLVTG-ELLT 623
              VEL G KG+W++     + SI         D+ +  +L++S  + T +L    E   
Sbjct: 401 QASVELQGIKGMWSL-----KSSI---------DEAFDTFLVVSFISETRILAMNIEDEL 460

Query: 624 EVTESVDYFVHGRTIAAGNLFGRRRVIQVYESGARILDGSFMTQDLNLVVNGNESGNASE 683
           E TE   +    +T+   +     +++QV  +  R++  S  T++L          +A  
Sbjct: 461 EETEIEGFLSEVQTLFCHDAV-YNQLVQVTSNSVRLV--SSTTREL------RNKWDAPA 520

Query: 684 GCTVLSASISDPYVLLTMTDGS-IRLLVGDSSSCSV 718
           G +V  A+ +   VLL    G  + L +GD +   V
Sbjct: 521 GFSVNVATANASQVLLATGGGHLVYLEIGDGTLTEV 523

BLAST of Cucsa.178510 vs. NCBI nr
Match: gi|778667872|ref|XP_011648998.1| (PREDICTED: cleavage and polyadenylation specificity factor subunit 1 [Cucumis sativus])

HSP 1 Score: 2820.8 bits (7311), Expect = 0.0e+00
Identity = 1427/1452 (98.28%), Postives = 1427/1452 (98.28%), Query Frame = 1

Query: 1    MSFAAYRMMHWPTGIENCDSAYITHSRADFVPAVTSHSDDLDSDWHPRRDIGPVPNLVVT 60
            MSFAAYRMMHWPTGIENCDSAYITHSRADFVPAVTSHSDDLDSDWHPRRDIGPVPNLVVT
Sbjct: 1    MSFAAYRMMHWPTGIENCDSAYITHSRADFVPAVTSHSDDLDSDWHPRRDIGPVPNLVVT 60

Query: 61   AGNVLEVYVVRVLEEGGRESKSSGEVRRGGIMDGVSGASLELVCHYRLHGNVESMAILSS 120
            AGNVLEVYVVRVLEEGGRESKSSGEVRRGGIMDGVSGASLELVCHYRLHGNVESMAILSS
Sbjct: 61   AGNVLEVYVVRVLEEGGRESKSSGEVRRGGIMDGVSGASLELVCHYRLHGNVESMAILSS 120

Query: 121  RGGDGSKKRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWLHLKRGRESFARGP 180
            RGGDGSKKRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWLHLKRGRESFARGP
Sbjct: 121  RGGDGSKKRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWLHLKRGRESFARGP 180

Query: 181  VVKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLINLRDL 240
            VVKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLINLRDL
Sbjct: 181  VVKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLINLRDL 240

Query: 241  DVKHVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALSISTTLKQHPLIWSA 300
            DVKHVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALSISTTLKQHPLIWSA
Sbjct: 241  DVKHVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALSISTTLKQHPLIWSA 300

Query: 301  SNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCMLALNNYAVSADSSQDMPRSNFN 360
            SNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCMLALNNYAVSADSSQDMPRSNFN
Sbjct: 301  SNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCMLALNNYAVSADSSQDMPRSNFN 360

Query: 361  VELDAANATWLVNDVALLSTKTGELLLLALVYDGRVVQRLDLSKSKASVLTSGIASIGNS 420
            VELDAANATWLVNDVALLSTKTGELLLLALVYDGRVVQRLDLSKSKASVLTSGIASIGNS
Sbjct: 361  VELDAANATWLVNDVALLSTKTGELLLLALVYDGRVVQRLDLSKSKASVLTSGIASIGNS 420

Query: 421  LFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMRRSSSDALQDMVGG 480
            LFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMRRSSSDALQDMVGG
Sbjct: 421  LFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMRRSSSDALQDMVGG 480

Query: 481  DELSLYGSAANNTESAQKIFSFAVRDSLINIGPLKDFSYGLRINADPNATGIAKQSNYEL 540
            DELSLYGSAANNTESAQKIFSFAVRDSLINIGPLKDFSYGLRINADPNATGIAKQSNYEL
Sbjct: 481  DELSLYGSAANNTESAQKIFSFAVRDSLINIGPLKDFSYGLRINADPNATGIAKQSNYEL 540

Query: 541  VCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVYHKNTRGSIADSSRTVPDDDEY 600
            VCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVYHKNTRGSIADSSR VPDDDEY
Sbjct: 541  VCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVYHKNTRGSIADSSRMVPDDDEY 600

Query: 601  HAYLIISLEARTMVLVTGELLTEVTESVDYFVHGRTIAAGNLFGRRRVIQVYESGARILD 660
            HAYLIISLEARTMVLVTGELLTEVTESVDYFVHGRTIAAGNLFGRRRVIQVYESGARILD
Sbjct: 601  HAYLIISLEARTMVLVTGELLTEVTESVDYFVHGRTIAAGNLFGRRRVIQVYESGARILD 660

Query: 661  GSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMTDGSIRLLVGDSSSCSVSVS 720
            GSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMTDGSIRLLVGDSSSCSVSVS
Sbjct: 661  GSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMTDGSIRLLVGDSSSCSVSVS 720

Query: 721  APAAFGSSKKCVSSCTLYQDKGFEPWLRMTSTDAWLSTGVGETIDGTDGSLQDQGDIYCV 780
            APAAFGSSKKCVSSCTLYQDKG EPWLRMTSTDAWLSTGVGETIDGTDGSLQDQGDIYCV
Sbjct: 721  APAAFGSSKKCVSSCTLYQDKGIEPWLRMTSTDAWLSTGVGETIDGTDGSLQDQGDIYCV 780

Query: 781  ACYDNGDLEIFDVPNFTSVFYVDKFVSGKSHLVDHQISDLQKSSEVDQNSQELISHGRNE 840
            ACYDNGDLEIFDVPNFTSVFYVDKFVSGKSHLVDHQISDLQKSSEVDQNSQELISHGRNE
Sbjct: 781  ACYDNGDLEIFDVPNFTSVFYVDKFVSGKSHLVDHQISDLQKSSEVDQNSQELISHGRNE 840

Query: 841  SSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFESTDSASKIDDSVSIDNSV 900
            SSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFESTDSASKIDDSVSIDNSV
Sbjct: 841  SSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFESTDSASKIDDSVSIDNSV 900

Query: 901  SSSNMSSSRLRNLRFLRVPLDIQGREDMPNGTLSRRLSIFKNISGYQGLFLCGSRPAWFM 960
            SSSNMSSSRLRNLRFLRVPLDIQGREDMPNGTLS RLSIFKNISGYQGLFLCGSRPAWFM
Sbjct: 901  SSSNMSSSRLRNLRFLRVPLDIQGREDMPNGTLSCRLSIFKNISGYQGLFLCGSRPAWFM 960

Query: 961  VFRERLRVHPQLCDGPIVAFAVLHNVNCNHGLIYVTSQ---------------------- 1020
            VFRERLRVHPQLCDGPIVAFAVLHNVNCNHGLIYVTSQ                      
Sbjct: 961  VFRERLRVHPQLCDGPIVAFAVLHNVNCNHGLIYVTSQGVLKICQLPSTSNYDNYWPVQK 1020

Query: 1021 VPLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQVLSSMVDQDVGHVENHNLSADELQQTY 1080
            VPLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQVLSSMVDQDVGHVENHNLSADELQQTY
Sbjct: 1021 VPLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQVLSSMVDQDVGHVENHNLSADELQQTY 1080

Query: 1081 SVEEFEIRILEPEKSGGPWQTRATIAMHSSENALTIRVVTLLNTTTKENETLLAVGTAYV 1140
            SVEEFEIRILEPEKSGGPWQTRATIAMHSSENALTIRVVTLLNTTTKENETLLAVGTAYV
Sbjct: 1081 SVEEFEIRILEPEKSGGPWQTRATIAMHSSENALTIRVVTLLNTTTKENETLLAVGTAYV 1140

Query: 1141 QGEDVAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALASLQGHLLIASGPKIILH 1200
            QGEDVAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALASLQGHLLIASGPKIILH
Sbjct: 1141 QGEDVAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALASLQGHLLIASGPKIILH 1200

Query: 1201 KWTGAELNGIAFYDVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLLAKDFGS 1260
            KWTGAELNGIAFYDVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLLAKDFGS
Sbjct: 1201 KWTGAELNGIAFYDVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLLAKDFGS 1260

Query: 1261 LDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTESWKGQKLLSRAEFHVGAHVTKFL 1320
            LDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTESWKGQKLLSRAEFHVGAHVTKFL
Sbjct: 1261 LDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTESWKGQKLLSRAEFHVGAHVTKFL 1320

Query: 1321 RLQMLSTSSDKACSTVSDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKLGDAVP 1380
            RLQMLSTSSDKACSTVSDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKLGDAVP
Sbjct: 1321 RLQMLSTSSDKACSTVSDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKLGDAVP 1380

Query: 1381 HVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELLCHYEMLPLEEQLDIAHQIGTTRSQILS 1431
            HVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELLCHYEMLPLEEQLDIAHQIGTTRSQILS
Sbjct: 1381 HVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELLCHYEMLPLEEQLDIAHQIGTTRSQILS 1440

BLAST of Cucsa.178510 vs. NCBI nr
Match: gi|659082456|ref|XP_008441850.1| (PREDICTED: cleavage and polyadenylation specificity factor subunit 1 isoform X1 [Cucumis melo])

HSP 1 Score: 2792.7 bits (7238), Expect = 0.0e+00
Identity = 1409/1452 (97.04%), Postives = 1419/1452 (97.73%), Query Frame = 1

Query: 1    MSFAAYRMMHWPTGIENCDSAYITHSRADFVPAVTSHSDDLDSDWHPRRDIGPVPNLVVT 60
            MSFAAYRMMHWPTGIENCDSA+ITHSRADFVPAVTSHSDDLDSDWHPRRDIGPVPNLVVT
Sbjct: 1    MSFAAYRMMHWPTGIENCDSAFITHSRADFVPAVTSHSDDLDSDWHPRRDIGPVPNLVVT 60

Query: 61   AGNVLEVYVVRVLEEGGRESKSSGEVRRGGIMDGVSGASLELVCHYRLHGNVESMAILSS 120
            AGNVLEVYVVRVLEEGGRES+SSGEVRRGGIMDGVSGASLELVCHYRLHGNVESMAILSS
Sbjct: 61   AGNVLEVYVVRVLEEGGRESRSSGEVRRGGIMDGVSGASLELVCHYRLHGNVESMAILSS 120

Query: 121  RGGDGSKKRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWLHLKRGRESFARGP 180
            RGGDGSKKRDSIILVFQEAKISVLEFDDS HSLRTSSMHCF+GPQWLHLKRGRESFARGP
Sbjct: 121  RGGDGSKKRDSIILVFQEAKISVLEFDDSIHSLRTSSMHCFEGPQWLHLKRGRESFARGP 180

Query: 181  VVKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLINLRDL 240
            VVKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLINLRDL
Sbjct: 181  VVKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLINLRDL 240

Query: 241  DVKHVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALSISTTLKQHPLIWSA 300
            DVKHVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCM+SALSISTTLKQHPLIWSA
Sbjct: 241  DVKHVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMISALSISTTLKQHPLIWSA 300

Query: 301  SNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCMLALNNYAVSADSSQDMPRSNFN 360
            +NLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCMLALNNYAVSADSSQDMPRSNFN
Sbjct: 301  NNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCMLALNNYAVSADSSQDMPRSNFN 360

Query: 361  VELDAANATWLVNDVALLSTKTGELLLLALVYDGRVVQRLDLSKSKASVLTSGIASIGNS 420
            VELDAANATWLVNDVALLSTKTGELLLLALVYDGRVVQRLDLSKSKASVL SGIASIGNS
Sbjct: 361  VELDAANATWLVNDVALLSTKTGELLLLALVYDGRVVQRLDLSKSKASVLASGIASIGNS 420

Query: 421  LFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMRRSSSDALQDMVGG 480
            LFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMRRSSSDALQDMVGG
Sbjct: 421  LFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMRRSSSDALQDMVGG 480

Query: 481  DELSLYGSAANNTESAQKIFSFAVRDSLINIGPLKDFSYGLRINADPNATGIAKQSNYEL 540
            DELSLYGSAANNTESAQK FSFAVRDSLINIGPLKDFSYGLRINADPNATGIAKQSNYEL
Sbjct: 481  DELSLYGSAANNTESAQKNFSFAVRDSLINIGPLKDFSYGLRINADPNATGIAKQSNYEL 540

Query: 541  VCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVYHKNTRGSIADSSRTVPDDDEY 600
            VCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVYHKNTRGSIADSSR VPDDDEY
Sbjct: 541  VCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVYHKNTRGSIADSSRMVPDDDEY 600

Query: 601  HAYLIISLEARTMVLVTGELLTEVTESVDYFVHGRTIAAGNLFGRRRVIQVYESGARILD 660
            HAYLIISLEARTMVL TG+LLTEVTE+VDYFVHGRTIAAGNLFGRRRVIQVYESGARILD
Sbjct: 601  HAYLIISLEARTMVLETGDLLTEVTETVDYFVHGRTIAAGNLFGRRRVIQVYESGARILD 660

Query: 661  GSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMTDGSIRLLVGDSSSCSVSVS 720
            GSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMTDGSIRLLVGDSSSCSVSVS
Sbjct: 661  GSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMTDGSIRLLVGDSSSCSVSVS 720

Query: 721  APAAFGSSKKCVSSCTLYQDKGFEPWLRMTSTDAWLSTGVGETIDGTDGSLQDQGDIYCV 780
            APAAFGSSKKCVSSCTLY DKG EPWLRMTSTDAWLSTGVGETIDGTDGSLQDQGDIYCV
Sbjct: 721  APAAFGSSKKCVSSCTLYHDKGVEPWLRMTSTDAWLSTGVGETIDGTDGSLQDQGDIYCV 780

Query: 781  ACYDNGDLEIFDVPNFTSVFYVDKFVSGKSHLVDHQISDLQKSSEVDQNSQELISHGRNE 840
            ACYDNGDLEIFDVPNF SVFYVDKFVSGKSHLVDHQISDLQK SEVDQNSQELISHGRNE
Sbjct: 781  ACYDNGDLEIFDVPNFISVFYVDKFVSGKSHLVDHQISDLQKPSEVDQNSQELISHGRNE 840

Query: 841  SSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFESTDSASKIDDSVSIDNSV 900
            SSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFE+TDSASKIDDSVSIDNSV
Sbjct: 841  SSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFENTDSASKIDDSVSIDNSV 900

Query: 901  SSSNMSSSRLRNLRFLRVPLDIQGREDMPNGTLSRRLSIFKNISGYQGLFLCGSRPAWFM 960
            SSSNMSSSRLRNLRFLRVPLDIQGR+DMPNGTLSRRLSIFKNISGYQGLFLCGSRPAWFM
Sbjct: 901  SSSNMSSSRLRNLRFLRVPLDIQGRDDMPNGTLSRRLSIFKNISGYQGLFLCGSRPAWFM 960

Query: 961  VFRERLRVHPQLCDGPIVAFAVLHNVNCNHGLIYVTSQ---------------------- 1020
            VFRERLRVHPQLCDGPIVAF VLHNVNCNHGLIYVTSQ                      
Sbjct: 961  VFRERLRVHPQLCDGPIVAFTVLHNVNCNHGLIYVTSQGVLKICQLPSTSNYDNYWPVQK 1020

Query: 1021 VPLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQVLSSMVDQDVGHVENHNLSADELQQTY 1080
            VPLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQVLSSMVDQDVGHVENHNLSADELQQTY
Sbjct: 1021 VPLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQVLSSMVDQDVGHVENHNLSADELQQTY 1080

Query: 1081 SVEEFEIRILEPEKSGGPWQTRATIAMHSSENALTIRVVTLLNTTTKENETLLAVGTAYV 1140
            SVEEFEIRILEPEKSGGPWQTRATIAMHSSENALTIRVVTLLN TTKENETLLAVGTAYV
Sbjct: 1081 SVEEFEIRILEPEKSGGPWQTRATIAMHSSENALTIRVVTLLNATTKENETLLAVGTAYV 1140

Query: 1141 QGEDVAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALASLQGHLLIASGPKIILH 1200
            QGEDVAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALASLQGHLLIASGPKIILH
Sbjct: 1141 QGEDVAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALASLQGHLLIASGPKIILH 1200

Query: 1201 KWTGAELNGIAFYDVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLLAKDFGS 1260
            KWTGAELNGIAFYDVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLLAKDFGS
Sbjct: 1201 KWTGAELNGIAFYDVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLLAKDFGS 1260

Query: 1261 LDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTESWKGQKLLSRAEFHVGAHVTKFL 1320
            LDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTESWKGQKLLSRAEFHVGAHVTKFL
Sbjct: 1261 LDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTESWKGQKLLSRAEFHVGAHVTKFL 1320

Query: 1321 RLQMLSTSSDKACSTVSDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKLGDAVP 1380
            RLQMLSTSSDKACSTVSDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKLGDAVP
Sbjct: 1321 RLQMLSTSSDKACSTVSDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKLGDAVP 1380

Query: 1381 HVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELLCHYEMLPLEEQLDIAHQIGTTRSQILS 1431
            HVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELLCHYEMLPLEEQL+IAHQIGTTRSQILS
Sbjct: 1381 HVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELLCHYEMLPLEEQLEIAHQIGTTRSQILS 1440

BLAST of Cucsa.178510 vs. NCBI nr
Match: gi|590671948|ref|XP_007038473.1| (Cleavage and polyadenylation specificity factor 160 isoform 1 [Theobroma cacao])

HSP 1 Score: 2331.2 bits (6040), Expect = 0.0e+00
Identity = 1156/1457 (79.34%), Postives = 1289/1457 (88.47%), Query Frame = 1

Query: 1    MSFAAYRMMHWPTGIENCDSAYITHSRADFVPAVT-SHSDDLDSDWHPRRDIGPVPNLVV 60
            MS+AAY+MMHWPTGIENC S ++TH RADF P +  + ++DL+S+W  RR IGPVPNL+V
Sbjct: 1    MSYAAYKMMHWPTGIENCASGFVTHCRADFTPQIPLNQTEDLESEWPARRGIGPVPNLIV 60

Query: 61   TAGNVLEVYVVRVLEEGGRESKSSGEVRRGGIMDGVSGASLELVCHYRLHGNVESMAILS 120
            TA N+LE+YVVRV EEG RE+++S EV+RGG++DGVSG SLELVC+YRLHGNVESMA+LS
Sbjct: 61   TAANLLEIYVVRVQEEGRREARNSTEVKRGGVLDGVSGVSLELVCNYRLHGNVESMAVLS 120

Query: 121  SRGGDGSKKRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWLHLKRGRESFARG 180
              GGDGS++RDSIIL F++AKISVLEFDDS H LRT+SMHCF+GP+WLHLKRGRESFARG
Sbjct: 121  IGGGDGSRRRDSIILAFKDAKISVLEFDDSIHGLRTTSMHCFEGPEWLHLKRGRESFARG 180

Query: 181  PVVKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLINLRD 240
            P+VKVDPQGRCGGVLVY LQMIILKASQAGSG V +D+AFG+ GA+SARVESSY+INLRD
Sbjct: 181  PLVKVDPQGRCGGVLVYDLQMIILKASQAGSGFVGEDDAFGSGGAVSARVESSYIINLRD 240

Query: 241  LDVKHVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALSISTTLKQHPLIWS 300
            LDVKH+KDF+FVHGYIEPVMVILHE+ELTWAGRVSWKHHTCM+SALSISTTLKQHPLIWS
Sbjct: 241  LDVKHIKDFIFVHGYIEPVMVILHERELTWAGRVSWKHHTCMISALSISTTLKQHPLIWS 300

Query: 301  ASNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCMLALNNYAVSADSSQDMPRSNF 360
            A NLPHDAYKLLAVPSPIGGVLVISAN+IHY+SQSASC LALNNYA+S D+SQD+PRSNF
Sbjct: 301  AVNLPHDAYKLLAVPSPIGGVLVISANTIHYHSQSASCALALNNYAISVDNSQDLPRSNF 360

Query: 361  NVELDAANATWLVNDVALLSTKTGELLLLALVYDGRVVQRLDLSKSKASVLTSGIASIGN 420
            +VELDAANATWL+NDVALLSTKTGELLLL L+YDGRVVQRLDLSKSKASVLTS I +IGN
Sbjct: 361  SVELDAANATWLLNDVALLSTKTGELLLLTLIYDGRVVQRLDLSKSKASVLTSDITTIGN 420

Query: 421  SLFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMRRSSSDALQDMVG 480
            SLFFLGSRLGDSLLVQFS G G S L S LK+E GDIE D   AKR+RRSSSDALQDMVG
Sbjct: 421  SLFFLGSRLGDSLLVQFSGGSGVSALPSGLKEEVGDIEGDVPLAKRLRRSSSDALQDMVG 480

Query: 481  GDELSLYGSAANNTESAQKIFSFAVRDSLINIGPLKDFSYGLRINADPNATGIAKQSNYE 540
            G+ELSLYGSA NNTESAQK F FAVRDSL N+GPLKDFSYGLRINAD NATGIAKQSNYE
Sbjct: 481  GEELSLYGSAPNNTESAQKTFLFAVRDSLTNVGPLKDFSYGLRINADVNATGIAKQSNYE 540

Query: 541  LVCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVYHKNTRGSIADSSRTVPDDDE 600
            LVCCSGHGKNGALC+LRQSIRPEMITEVEL GCKGIWTVYHK+TR   AD S+   DDDE
Sbjct: 541  LVCCSGHGKNGALCVLRQSIRPEMITEVELTGCKGIWTVYHKSTRSHSADLSKVTDDDDE 600

Query: 601  YHAYLIISLEARTMVLVTGELLTEVTESVDYFVHGRTIAAGNLFGRRRVIQVYESGARIL 660
            YHAYLIISLEARTMVL T +LLTEVTESVDY+V GRTIAAGNLFGRRRV+QVYE GARIL
Sbjct: 601  YHAYLIISLEARTMVLETADLLTEVTESVDYYVQGRTIAAGNLFGRRRVVQVYERGARIL 660

Query: 661  DGSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMTDGSIRLLVGDSSSCSVSV 720
            DGSFMTQ+L++    +ES   SE  TV+S SI+DPYVLL MTDGSI LLVGD ++C+VS+
Sbjct: 661  DGSFMTQELSIPSPNSESSPGSENSTVISVSIADPYVLLRMTDGSILLLVGDPATCTVSI 720

Query: 721  SAPAAFGSSKKCVSSCTLYQDKGFEPWLRMTSTDAWLSTGVGETIDGTDGSLQDQGDIYC 780
            + P AF  SKK VS+CTLY DKG EPWLR  STDAWLSTGVGE+IDG DG   DQGDIYC
Sbjct: 721  NTPTAFEGSKKMVSACTLYHDKGPEPWLRKASTDAWLSTGVGESIDGADGGPHDQGDIYC 780

Query: 781  VACYDNGDLEIFDVPNFTSVFYVDKFVSGKSHLVDHQISDLQKSSE--VDQNSQELISHG 840
            V CY++G LEIFDVPNF  VF ++KF SG++ LVD    +  K SE  ++++S+EL   G
Sbjct: 781  VVCYESGALEIFDVPNFNCVFSMEKFASGRTRLVDAYTLESSKDSEKVINKSSEELTGQG 840

Query: 841  RNESSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFESTDSASKIDDSVSID 900
            R E+ QN+KV+E+AMQRWS  HSRPFLFGILTDGTILCYHAYLFE +++ASK++DSV   
Sbjct: 841  RKENVQNLKVVELAMQRWSANHSRPFLFGILTDGTILCYHAYLFEGSENASKVEDSVVAQ 900

Query: 901  NSVSSSNMSSSRLRNLRFLRVPLDIQGREDMPNGTLSRRLSIFKNISGYQGLFLCGSRPA 960
            NSV  SN+++SRLRNLRF+R+PLD   RE+M NGTLS+R++IFKNISGYQG FL GSRPA
Sbjct: 901  NSVGLSNINASRLRNLRFIRIPLDAYTREEMSNGTLSQRITIFKNISGYQGFFLSGSRPA 960

Query: 961  WFMVFRERLRVHPQLCDGPIVAFAVLHNVNCNHGLIYVTSQ------------------- 1020
            WFMVFRERLRVHPQLCDG IVAF VLHNVNCNHG IYVTSQ                   
Sbjct: 961  WFMVFRERLRVHPQLCDGSIVAFTVLHNVNCNHGFIYVTSQGILKICQIPSASNYDNYWP 1020

Query: 1021 ---VPLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQVLSSMVDQDVGH-VENHNLSADEL 1080
               +PL+GTPHQVTYF E+NLYP+I+S PV KP+NQVLSS+VDQ+VGH ++NHNLS+DEL
Sbjct: 1021 VQKIPLRGTPHQVTYFAERNLYPIIVSVPVHKPVNQVLSSLVDQEVGHQMDNHNLSSDEL 1080

Query: 1081 QQTYSVEEFEIRILEPEKSGGPWQTRATIAMHSSENALTIRVVTLLNTTTKENETLLAVG 1140
            Q+TY+V+EFE+RILEPEKSGGPW+T+ATI M SSENALT+RVVTL NTTTKENE+LLA+G
Sbjct: 1081 QRTYTVDEFEVRILEPEKSGGPWETKATIPMQSSENALTVRVVTLFNTTTKENESLLAIG 1140

Query: 1141 TAYVQGEDVAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALASLQGHLLIASGPK 1200
            TAY+QGEDVAARGRV+L S+G++ DN Q LVSEVYSKELKGAISALASLQGHLLIASGPK
Sbjct: 1141 TAYIQGEDVAARGRVILCSIGRNTDNLQNLVSEVYSKELKGAISALASLQGHLLIASGPK 1200

Query: 1201 IILHKWTGAELNGIAFYDVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLLAK 1260
            IILH WTG+ELNGIAFYD PPLYVVSLNIVKNFILLGD+HKSIYFLSWKEQGAQLSLLAK
Sbjct: 1201 IILHNWTGSELNGIAFYDAPPLYVVSLNIVKNFILLGDVHKSIYFLSWKEQGAQLSLLAK 1260

Query: 1261 DFGSLDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTESWKGQKLLSRAEFHVGAHV 1320
            DFGSLDC+ATEFLIDGSTLSL VSD+QKNIQIFYYAPK +ESWKGQKLLSRAEFHVGAHV
Sbjct: 1261 DFGSLDCFATEFLIDGSTLSLMVSDEQKNIQIFYYAPKMSESWKGQKLLSRAEFHVGAHV 1320

Query: 1321 TKFLRLQMLSTSSDKACSTV-SDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKL 1380
            TKFLRLQMLSTSSD+  +T  SDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKL
Sbjct: 1321 TKFLRLQMLSTSSDRTSATAGSDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKKL 1380

Query: 1381 GDAVPHVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELLCHYEMLPLEEQLDIAHQIGTTR 1431
             DAVPHV GLNPRSFRQFHSNGK HR GPDSIVDCELLCHYEMLPLEEQLDIAHQIGTTR
Sbjct: 1381 VDAVPHVAGLNPRSFRQFHSNGKAHRPGPDSIVDCELLCHYEMLPLEEQLDIAHQIGTTR 1440

BLAST of Cucsa.178510 vs. NCBI nr
Match: gi|1009122186|ref|XP_015877866.1| (PREDICTED: cleavage and polyadenylation specificity factor subunit 1 [Ziziphus jujuba])

HSP 1 Score: 2315.0 bits (5998), Expect = 0.0e+00
Identity = 1153/1458 (79.08%), Postives = 1290/1458 (88.48%), Query Frame = 1

Query: 1    MSFAAYRMMHWPTGIENCDSAYITHSRADFVPAVTS-HSDDLDSDWHP-RRDIGPVPNLV 60
            MSFAA++MMHWPTGIENC S +ITHSRADFVP +    +DDLDSDW   RR+IGP+PNLV
Sbjct: 1    MSFAAFKMMHWPTGIENCASGFITHSRADFVPRIPPIQNDDLDSDWSASRREIGPIPNLV 60

Query: 61   VTAGNVLEVYVVRVLEEGGRESKSSGEVRRGGIMDGVSGASLELVCHYRLHGNVESMAIL 120
            VTAGNVLEVYVVR+ EE  R S++SGE RRGG+MDG+SGASLELVCHYRLHGNVE+MA+L
Sbjct: 61   VTAGNVLEVYVVRIQEESNRSSRASGESRRGGVMDGLSGASLELVCHYRLHGNVETMAVL 120

Query: 121  SSRGGDGSKKRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWLHLKRGRESFAR 180
            S+ GG+ S++RDSIIL FQ+AKISVL+FDDSTH LRTSSMHCF+GP+WLHLKRGRESFAR
Sbjct: 121  STGGGESSRRRDSIILSFQDAKISVLDFDDSTHGLRTSSMHCFEGPKWLHLKRGRESFAR 180

Query: 181  GPVVKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLINLR 240
            GP+VKVDPQGRCGGVLVY  QMIILKA+QAGSGLVVD++   + GA+SA +ESSY+INLR
Sbjct: 181  GPLVKVDPQGRCGGVLVYDFQMIILKAAQAGSGLVVDEDTSSSGGAVSAHIESSYIINLR 240

Query: 241  DLDVKHVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALSISTTLKQHPLIW 300
            DLD+KH+KDF+FVHGYIEPVMVILHE+ELTWAGRV+WKHHTCMVSALSISTTLKQHPLIW
Sbjct: 241  DLDMKHIKDFIFVHGYIEPVMVILHERELTWAGRVAWKHHTCMVSALSISTTLKQHPLIW 300

Query: 301  SASNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCMLALNNYAVSADSSQDMPRSN 360
            SA+NLPHDAYKLLAVPSPIGGVLVI ANSIHY+SQS SC LALNN+AVS DSSQ+MPRS+
Sbjct: 301  SAANLPHDAYKLLAVPSPIGGVLVIGANSIHYHSQSTSCALALNNFAVSVDSSQEMPRSS 360

Query: 361  FNVELDAANATWLVNDVALLSTKTGELLLLALVYDGRVVQRLDLSKSKASVLTSGIASIG 420
            FNVELDAANATWL+NDVALLSTKTGELLLL +VYDGRVVQRLDLSKSKASVLTSGI +IG
Sbjct: 361  FNVELDAANATWLLNDVALLSTKTGELLLLTIVYDGRVVQRLDLSKSKASVLTSGITTIG 420

Query: 421  NSLFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMRRSSSDALQDMV 480
            NSLFFLGSRLGDSLLVQF+CGVGSS ++S LKDE GDIE DA +AKR+RR SSDA QDM 
Sbjct: 421  NSLFFLGSRLGDSLLVQFTCGVGSSIMSSALKDEVGDIEGDAPSAKRLRRLSSDASQDMA 480

Query: 481  GGDELSLYGSAANNTESAQKIFSFAVRDSLINIGPLKDFSYGLRINADPNATGIAKQSNY 540
             G+ELSLYGSA NNTESAQK FSFAVRDSLIN+GP+KDFSYGLR+NAD NATGIAKQSNY
Sbjct: 481  SGEELSLYGSAPNNTESAQKSFSFAVRDSLINVGPIKDFSYGLRVNADTNATGIAKQSNY 540

Query: 541  ELVCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVYHKNTRGSIADSSRTVPDDD 600
            ELVCCSGHGKNGALC+LRQSIRPEMITEVELPGCKGIWTVYHK+TRG   DS+++   DD
Sbjct: 541  ELVCCSGHGKNGALCVLRQSIRPEMITEVELPGCKGIWTVYHKSTRGHNVDSAKSAAADD 600

Query: 601  EYHAYLIISLEARTMVLVTGELLTEVTESVDYFVHGRTIAAGNLFGRRRVIQVYESGARI 660
            EYHAYLIISLEARTMVL T +LLTEVTESVDY+V GRTIAAGNLFGRRRV+QVYE GARI
Sbjct: 601  EYHAYLIISLEARTMVLETADLLTEVTESVDYYVQGRTIAAGNLFGRRRVVQVYERGARI 660

Query: 661  LDGSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMTDGSIRLLVGDSSSCSVS 720
            LDGSFMTQDL++V   +ESG  SE  TVLS SI+DPYV+L MTDGSIRLL+GD SSC+VS
Sbjct: 661  LDGSFMTQDLSIVAANSESG--SESATVLSVSIADPYVVLRMTDGSIRLLIGDPSSCTVS 720

Query: 721  VSAPAAFGSSKKCVSSCTLYQDKGFEPWLRMTSTDAWLSTGVGETIDGTDGSLQDQGDIY 780
            +S PAAF SSKK +S+CTLY D G EPWLR TSTDAWLSTGV E +DG DGSL DQGDIY
Sbjct: 721  ISTPAAFESSKKLISACTLYHDDGPEPWLRKTSTDAWLSTGVDEAVDGADGSLHDQGDIY 780

Query: 781  CVACYDNGDLEIFDVPNFTSVFYVDKFVSGKSHLVDHQISDLQKSSE--VDQNSQELISH 840
            CV CY++G LEI+DVPNF  VF V+KF+SGK +L+D  + +  K  +  ++++S+++   
Sbjct: 781  CVVCYESGSLEIYDVPNFNCVFSVEKFISGKMNLLDTLVEEQSKDPQKLMNRSSEDVSGQ 840

Query: 841  GRNESSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFESTDSASKIDDSVSI 900
             R E+ QNMK++E+AMQRWSGQHSRPFLFGIL+DGTILCYHAYLFE  +SASK +DSVS 
Sbjct: 841  ARKENVQNMKIVELAMQRWSGQHSRPFLFGILSDGTILCYHAYLFEGPESASKTEDSVSA 900

Query: 901  DNSVSSSNMSSSRLRNLRFLRVPLDIQGREDMPNGTLSRRLSIFKNISGYQGLFLCGSRP 960
             +    SN S+SRLRNLRF+RV LD   +E+ PN T  +R+SIFKNI+GYQGLFL GSRP
Sbjct: 901  QSLSGLSNNSASRLRNLRFVRVALDTYAKEETPNATSCQRISIFKNIAGYQGLFLSGSRP 960

Query: 961  AWFMVFRERLRVHPQLCDGPIVAFAVLHNVNCNHGLIYVTSQ------------------ 1020
            AWFMVFRERLRVHPQLCDG IVAF VLHNVNCNHGLIYVTSQ                  
Sbjct: 961  AWFMVFRERLRVHPQLCDGSIVAFTVLHNVNCNHGLIYVTSQGILKICQLPSITSYDSYW 1020

Query: 1021 ----VPLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQVLSSMVDQDVGH-VENHNLSADE 1080
                +PLKGTPHQVTYF EKNLYP+I+S PV KPLNQV+SS++DQ+VGH  ENHNLS+D+
Sbjct: 1021 PVQKIPLKGTPHQVTYFAEKNLYPLIVSVPVHKPLNQVISSLIDQEVGHQAENHNLSSDD 1080

Query: 1081 LQQTYSVEEFEIRILEPEKSGGPWQTRATIAMHSSENALTIRVVTLLNTTTKENETLLAV 1140
            L +TY+V+EFE+RILEPE SGGPWQT+ATI M +SENALT+RVVTL NTTTKENETLLA+
Sbjct: 1081 LHRTYTVDEFEVRILEPEISGGPWQTKATIPMQTSENALTVRVVTLFNTTTKENETLLAI 1140

Query: 1141 GTAYVQGEDVAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALASLQGHLLIASGP 1200
            GTAYVQGEDVAARGRVLLFS+G   +N Q LVSEVY+K+LKGAISALASLQGHLL+ASGP
Sbjct: 1141 GTAYVQGEDVAARGRVLLFSIG---NNPQNLVSEVYTKDLKGAISALASLQGHLLMASGP 1200

Query: 1201 KIILHKWTGAELNGIAFYDVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLLA 1260
            KIILHKWTG ELN +AF+DVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLLA
Sbjct: 1201 KIILHKWTGGELNAVAFFDVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLLA 1260

Query: 1261 KDFGSLDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTESWKGQKLLSRAEFHVGAH 1320
            KDFGSLDC+ATEFLIDGSTLSL VSD++KNIQIFYYAPK +ESWKGQKLLSRAEFHVGAH
Sbjct: 1261 KDFGSLDCFATEFLIDGSTLSLVVSDNRKNIQIFYYAPKMSESWKGQKLLSRAEFHVGAH 1320

Query: 1321 VTKFLRLQMLSTSSDK-ACSTVSDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQKK 1380
            VTK LRLQMLST+SD+   ++VSDKTNRFALLFGTLDGS+GCIAPLDELTFRRLQSLQKK
Sbjct: 1321 VTKLLRLQMLSTTSDRTGTASVSDKTNRFALLFGTLDGSVGCIAPLDELTFRRLQSLQKK 1380

Query: 1381 LGDAVPHVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELLCHYEMLPLEEQLDIAHQIGTT 1431
            L DAV HV GLNPRSFRQF SNGK HR GPDSIVDCELLCHYEMLPLEEQL+IAHQIGTT
Sbjct: 1381 LVDAVSHVAGLNPRSFRQFRSNGKAHRPGPDSIVDCELLCHYEMLPLEEQLEIAHQIGTT 1440

BLAST of Cucsa.178510 vs. NCBI nr
Match: gi|645257300|ref|XP_008234350.1| (PREDICTED: cleavage and polyadenylation specificity factor subunit 1 [Prunus mume])

HSP 1 Score: 2310.4 bits (5986), Expect = 0.0e+00
Identity = 1154/1459 (79.10%), Postives = 1282/1459 (87.87%), Query Frame = 1

Query: 1    MSFAAYRMMHWPTGIENCDSAYITHSRADFVPAVTS-HSDDLDSDWHP-RRDIGPVPNLV 60
            MSFAAY+MMHWPTGIENC S +I+HSR+DFVP +    ++DL+S+W   RR+IGP+P+LV
Sbjct: 1    MSFAAYKMMHWPTGIENCASGFISHSRSDFVPRILPIQTEDLESEWPTSRREIGPIPDLV 60

Query: 61   VTAGNVLEVYVVRVLEEGG-RESKSSGEVRRGGIMDGVSGASLELVCHYRLHGNVESMAI 120
            VTAGNVLEVYVVRV EE G R  ++SGE +RGG+MDGVSGASLELVCHYRLHGNV +MA+
Sbjct: 61   VTAGNVLEVYVVRVQEEDGTRGPRASGEPKRGGLMDGVSGASLELVCHYRLHGNVVTMAV 120

Query: 121  LSSRGGDGSKKRDSIILVFQEAKISVLEFDDSTHSLRTSSMHCFDGPQWLHLKRGRESFA 180
            LSS GGDGS++RDSIIL F++AKISVLEFDDS H LRTSSMHCF+GP+WLHL+RGRESFA
Sbjct: 121  LSSGGGDGSRRRDSIILTFEDAKISVLEFDDSIHGLRTSSMHCFEGPEWLHLRRGRESFA 180

Query: 181  RGPVVKVDPQGRCGGVLVYGLQMIILKASQAGSGLVVDDEAFGNTGAISARVESSYLINL 240
            RGP+VKVDPQGRCG +LVYGLQMIILKASQ GSGLV DD++FG+ GAISAR+ESSY++NL
Sbjct: 181  RGPLVKVDPQGRCGSILVYGLQMIILKASQGGSGLVGDDDSFGSGGAISARIESSYIVNL 240

Query: 241  RDLDVKHVKDFVFVHGYIEPVMVILHEQELTWAGRVSWKHHTCMVSALSISTTLKQHPLI 300
            RD+D+KHVKDF F+HGYIEPVMVILHEQELTWAGRVSWKHHTCM+SALSISTTLKQHPLI
Sbjct: 241  RDMDMKHVKDFTFLHGYIEPVMVILHEQELTWAGRVSWKHHTCMISALSISTTLKQHPLI 300

Query: 301  WSASNLPHDAYKLLAVPSPIGGVLVISANSIHYNSQSASCMLALNNYAVSADSSQDMPRS 360
            WSA NLPHDAYKLLAVPSPIGGVLVISANSIHY+SQSASC LALN+YAVSAD+SQ++PRS
Sbjct: 301  WSAVNLPHDAYKLLAVPSPIGGVLVISANSIHYHSQSASCALALNSYAVSADNSQEVPRS 360

Query: 361  NFNVELDAANATWLVNDVALLSTKTGELLLLALVYDGRVVQRLDLSKSKASVLTSGIASI 420
            +F VELDAANATWL+NDVALLSTKTGELLLL LVYDGRVVQRLDLSKSKASVLTSGI  +
Sbjct: 361  SFPVELDAANATWLLNDVALLSTKTGELLLLTLVYDGRVVQRLDLSKSKASVLTSGITKV 420

Query: 421  GNSLFFLGSRLGDSLLVQFSCGVGSSGLASNLKDEGGDIEVDAHTAKRMRRSSSDALQDM 480
            GNSLFFLGSRLGDSLLVQF+CGVG S L+S++KDE GDIE DA +AKR+R SSSDALQDM
Sbjct: 421  GNSLFFLGSRLGDSLLVQFTCGVGGSVLSSDMKDEVGDIEGDAPSAKRLRMSSSDALQDM 480

Query: 481  VGGDELSLYGSAANNTESAQKIFSFAVRDSLINIGPLKDFSYGLRINADPNATGIAKQSN 540
            V G+ELSLYGSA NN ESAQK FSFAVRDSLIN+GPLKDFSYGLRINAD NATGIAKQSN
Sbjct: 481  VSGEELSLYGSAPNNAESAQKSFSFAVRDSLINVGPLKDFSYGLRINADANATGIAKQSN 540

Query: 541  YELVCCSGHGKNGALCILRQSIRPEMITEVELPGCKGIWTVYHKNTRGSIADSSRTVPDD 600
            YELVCCSGHGKNGALC+LRQSIRPEMITEVELPGCKGIWTVYHKN RG  ADSS+    D
Sbjct: 541  YELVCCSGHGKNGALCVLRQSIRPEMITEVELPGCKGIWTVYHKNARGHNADSSKIAASD 600

Query: 601  DEYHAYLIISLEARTMVLVTGELLTEVTESVDYFVHGRTIAAGNLFGRRRVIQVYESGAR 660
            DEYHAYLIISLEARTMVL T +LL+EVTESVDYFV GRTIAAGNLFGRRRV+QVYE GAR
Sbjct: 601  DEYHAYLIISLEARTMVLETADLLSEVTESVDYFVQGRTIAAGNLFGRRRVVQVYERGAR 660

Query: 661  ILDGSFMTQDLNLVVNGNESGNASEGCTVLSASISDPYVLLTMTDGSIRLLVGDSSSCSV 720
            ILDGSFMTQDL+   + +E G  SE  TVLS SI DPYVLL M+DG IRLLVGD S C+V
Sbjct: 661  ILDGSFMTQDLSFGTSNSEMGTGSESSTVLSVSIVDPYVLLRMSDGGIRLLVGDPSLCTV 720

Query: 721  SVSAPAAFGSSKKCVSSCTLYQDKGFEPWLRMTSTDAWLSTGVGETIDGTDGSLQDQGDI 780
            S+S PAAF SS K +S+CTLY DKG EPWLR TSTDAWLSTG+ E IDG DG   DQGD+
Sbjct: 721  SISIPAAFESSTKSISACTLYHDKGPEPWLRKTSTDAWLSTGIDEAIDGADGVSHDQGDV 780

Query: 781  YCVACYDNGDLEIFDVPNFTSVFYVDKFVSGKSHLVDHQISDLQKSSE--VDQNSQELIS 840
            YCV CY++G LEIFDVPNF  VF VDKFVSG +HLVD  + D  K  +  ++++S+E+  
Sbjct: 781  YCVVCYESGSLEIFDVPNFNCVFSVDKFVSGNAHLVDALMRDPPKDPQKLINKSSEEVSG 840

Query: 841  HGRNESSQNMKVIEVAMQRWSGQHSRPFLFGILTDGTILCYHAYLFESTDSASKIDDSVS 900
             GR E+ QNMKV+E+AMQRW GQHSRPFLFGIL DG ILCYHAYLFE  ++ASK +DS S
Sbjct: 841  QGRKENIQNMKVVELAMQRWLGQHSRPFLFGILNDGMILCYHAYLFEDPETASKTEDSAS 900

Query: 901  IDNSVSSSNMSSSRLRNLRFLRVPLDIQGREDMPNGTLSRRLSIFKNISGYQGLFLCGSR 960
              N+   SN+++SRLRNLRF+RVPLD   ++D  N T  +R++IFKNI+GYQGLFL GSR
Sbjct: 901  AQNTAGVSNLNASRLRNLRFVRVPLDTYAKKDTSNETSCQRMTIFKNIAGYQGLFLSGSR 960

Query: 961  PAWFMVFRERLRVHPQLCDGPIVAFAVLHNVNCNHGLIYVTSQ----------------- 1020
            PAWFMVFRERLR+HPQLCDG +VA  VLHNVNCNHGLIYVTSQ                 
Sbjct: 961  PAWFMVFRERLRIHPQLCDGSVVAVTVLHNVNCNHGLIYVTSQGILKICQLPPITSYDNY 1020

Query: 1021 -----VPLKGTPHQVTYFHEKNLYPVIISAPVQKPLNQVLSSMVDQDVGH-VENHNLSAD 1080
                 +PLKGTPHQVTYF EKNLYP+I+S PV KPLNQVLSS+VDQ+VGH VENHNLS+D
Sbjct: 1021 WPVQKIPLKGTPHQVTYFAEKNLYPLIVSVPVHKPLNQVLSSLVDQEVGHQVENHNLSSD 1080

Query: 1081 ELQQTYSVEEFEIRILEPEKSGGPWQTRATIAMHSSENALTIRVVTLLNTTTKENETLLA 1140
            EL +TYSV+EFEIRI+EP+KSGGPWQT+ATI M +SENALT+RVVTL NTTTKENETLLA
Sbjct: 1081 ELHRTYSVDEFEIRIMEPDKSGGPWQTKATIPMQTSENALTVRVVTLFNTTTKENETLLA 1140

Query: 1141 VGTAYVQGEDVAARGRVLLFSVGKDADNSQTLVSEVYSKELKGAISALASLQGHLLIASG 1200
            +GTAYVQGEDVA RGRVLLFS GK ADN+QTLVSEVYSKELKGAISALASLQGHLLIASG
Sbjct: 1141 IGTAYVQGEDVAGRGRVLLFSAGKSADNTQTLVSEVYSKELKGAISALASLQGHLLIASG 1200

Query: 1201 PKIILHKWTGAELNGIAFYDVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLL 1260
            PKIILHKW G ELNG+AF+DVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLL
Sbjct: 1201 PKIILHKWNGTELNGVAFFDVPPLYVVSLNIVKNFILLGDIHKSIYFLSWKEQGAQLSLL 1260

Query: 1261 AKDFGSLDCYATEFLIDGSTLSLTVSDDQKNIQIFYYAPKSTESWKGQKLLSRAEFHVGA 1320
            AKDFG+LDC+ATEFLIDGSTLSL V+D+QKNIQIFYYAPK +ESWKGQKLLSRAEFHVG 
Sbjct: 1261 AKDFGNLDCFATEFLIDGSTLSLVVADEQKNIQIFYYAPKMSESWKGQKLLSRAEFHVGT 1320

Query: 1321 HVTKFLRLQMLSTSSDK-ACSTVSDKTNRFALLFGTLDGSIGCIAPLDELTFRRLQSLQK 1380
            HVTKFLRLQMLSTSSD+   +  SDKTNR+ALLFGTLDGSIGCIAPLDELTFRRLQSLQK
Sbjct: 1321 HVTKFLRLQMLSTSSDRTGTNPGSDKTNRYALLFGTLDGSIGCIAPLDELTFRRLQSLQK 1380

Query: 1381 KLGDAVPHVGGLNPRSFRQFHSNGKVHRRGPDSIVDCELLCHYEMLPLEEQLDIAHQIGT 1431
            KL DAVPHV GLNPR+FRQF SNGK HR GPD+IVDCELL HYEMLPL EQL+IA+QIGT
Sbjct: 1381 KLVDAVPHVAGLNPRAFRQFRSNGKAHRPGPDTIVDCELLSHYEMLPLGEQLEIANQIGT 1440