Cucsa.177420 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.177420 vs. Swiss-Prot
Match: 4CL2_TOBAC (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum GN=4CL2 PE=1 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 7.4e-231
Identity = 401/541 (74.12%), Postives = 457/541 (84.47%), Query Frame = 1

Query: 29  ETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVDLTT 88
           +T + D+IFRSKLPDIYIP HLPLHSYC  EN ++   R CLING   + +TY DV+L +
Sbjct: 4   DTKQVDIIFRSKLPDIYIPNHLPLHSYCF-ENISEFSSRPCLINGANKQIYTYADVELNS 63

Query: 89  RKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQAKG 148
           RKVA+GL+K GI  +D IM+LLPNSPEFVFAF+GASYLGAI T ANP FT AE+ KQAK 
Sbjct: 64  RKVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKA 123

Query: 149 SKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDIDPE 208
           S +K+I+TQ+ +  KVK+   E  +VKI+ +DS P+GCL F  L +A+E ++P V+I P+
Sbjct: 124 SSAKIIVTQACHVNKVKDYAFE-NDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPD 183

Query: 209 DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHIYSL 268
           DVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY   EDV+LCVLPLFHIYSL
Sbjct: 184 DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSL 243

Query: 269 NSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYDLSS 328
           NSVLLCGLR G+ ILIM KF+I   L+L ++Y VT+ P VPPIVLAIAKSP ++ YDLSS
Sbjct: 244 NSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSS 303

Query: 329 IRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPGACG 388
           +R + SG APLGKELEDTVRAKFP A LGQGYGMTEAGPVL M LAFAK+PF +K GACG
Sbjct: 304 VRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACG 363

Query: 389 TVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDI 448
           TVVRNAEMKIVD +TG+SLPRN  GEICIRGDQIMKGYLN+PEATA TIDK+GWL+TGDI
Sbjct: 364 TVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDI 423

Query: 449 GFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVA 508
           G+IDDDDE+FIVDRLKELIKYKGFQVAPAELEALLL HP ISDAAVVPMKDEQAGEVPVA
Sbjct: 424 GYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVA 483

Query: 509 FVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAAFP 568
           FVV+   S  TEDE+K FISKQV+FYKRI RVFF+DAIPKSPSGKILRK+LRAKLAA  P
Sbjct: 484 FVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLP 542

Query: 569 N 570
           N
Sbjct: 544 N 542

BLAST of Cucsa.177420 vs. Swiss-Prot
Match: 4CL1_TOBAC (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum GN=4CL1 PE=2 SV=1)

HSP 1 Score: 799.7 bits (2064), Expect = 2.1e-230
Identity = 398/547 (72.76%), Postives = 457/547 (83.55%), Query Frame = 1

Query: 24  IEMGIETVEN-DVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYN 83
           +E   ET ++ D+IFRSKLPDIYIPKHLPLHSYC  EN ++   R CLING   + +TY 
Sbjct: 3   METTTETKQSGDLIFRSKLPDIYIPKHLPLHSYCF-ENISEFSSRPCLINGANDQIYTYA 62

Query: 84  DVDLTTRKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEI 143
           +V+LT RKVA GLNKLGI ++D IM+LLPNSPEFVFAF+GASYLGAI T ANP FT AE+
Sbjct: 63  EVELTCRKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEV 122

Query: 144 GKQAKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPR 203
            KQAK S +K+IITQS +  KVK+   E  +VK++ +DS P+GCL F +L ++DE E+P 
Sbjct: 123 VKQAKASSAKIIITQSCFVGKVKDYASE-NDVKVICIDSAPEGCLHFSELTQSDEHEIPE 182

Query: 204 VDIDPEDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPL 263
           V I P+DVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGEN NLY   EDV++CVLPL
Sbjct: 183 VKIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPL 242

Query: 264 FHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELE 323
           FHIYSLNS+LLCGLR G+ ILIM KF+I   L+L +KY V++ P VPPIVLAIAKSP ++
Sbjct: 243 FHIYSLNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVD 302

Query: 324 KYDLSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPM 383
            YDLSS+R + SG APLGKELED VR KFP A LGQGYGMTEAGPVL M LAFAK+PF +
Sbjct: 303 SYDLSSVRTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDI 362

Query: 384 KPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGW 443
           K GACGTVVRNAEMKIVD +TG SLPRN PGEICIRGDQIMKGYLN+PEAT  TIDK+GW
Sbjct: 363 KSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGW 422

Query: 444 LHTGDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQA 503
           LHTGDIGFID+DDE+FIVDRLKELIKYKGFQVAPAE+EALLL HP ISDAAVVPMKDEQA
Sbjct: 423 LHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQA 482

Query: 504 GEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAK 563
           GEVPVAFVV+   S  TEDE+K FISKQV+FYKR+ RVFF++ +PKSPSGKILRK+LRA+
Sbjct: 483 GEVPVAFVVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRAR 542

Query: 564 LAAAFPN 570
           LAA  PN
Sbjct: 543 LAAGVPN 547

BLAST of Cucsa.177420 vs. Swiss-Prot
Match: 4CL_VANPL (4-coumarate--CoA ligase OS=Vanilla planifolia GN=4CL PE=3 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 4.0e-229
Identity = 408/546 (74.73%), Postives = 457/546 (83.70%), Query Frame = 1

Query: 26  MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVD 85
           + IE  + D+IFRSKLPDIYIPK+LPLHSYC  EN +K   R CLING T E FTY DV+
Sbjct: 5   VAIEEQKKDIIFRSKLPDIYIPKNLPLHSYCF-ENISKFSSRPCLINGATDEIFTYADVE 64

Query: 86  LTTRKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQ 145
           L +R+V SGL+KLGI + D IM+LLPNSPEFVFAFLGAS++G+I T ANPFFT+ E+ KQ
Sbjct: 65  LISRRVGSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQ 124

Query: 146 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDG-----CLWFGDLIKADEREV 205
           AK S +KLIITQ  Y +KVK+   E   VKI+++D+          L F +L  ADE E+
Sbjct: 125 AKASNAKLIITQGCYVDKVKDYACE-NGVKIISIDTTTTADDAANILHFSELTGADENEM 184

Query: 206 PRVDIDPEDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVL 265
           P+V+I P+ VVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY   +DV+LCVL
Sbjct: 185 PKVEISPDGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVL 244

Query: 266 PLFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPE 325
           PLFHIYSLNSVLLCGLRAGS ILIM KFEI   L+L +KY VT+ P VPPIVLAIAKS  
Sbjct: 245 PLFHIYSLNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTV 304

Query: 326 LEKYDLSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPF 385
           ++ YDLSS+R + SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAK+PF
Sbjct: 305 VDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF 364

Query: 386 PMKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKD 445
            +K GACGTVVRNAEMKIVD ETGSSLPRN PGEICIRGDQIMKGYLN+PEATA TIDK+
Sbjct: 365 DIKSGACGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKE 424

Query: 446 GWLHTGDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDE 505
           GWLHTGDIG+IDDDDE+FIVDRLKELIKYKGFQVAPAELEALLLTHP ISDAAVVPMKDE
Sbjct: 425 GWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDE 484

Query: 506 QAGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELR 565
            AGEVPVAFVVK      TEDEIKQFISKQV+FYKRINRVFF++AIPK+PSGKILRK+LR
Sbjct: 485 AAGEVPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLR 544

Query: 566 AKLAAA 567
           A+LAAA
Sbjct: 545 ARLAAA 548

BLAST of Cucsa.177420 vs. Swiss-Prot
Match: 4CL2_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum GN=4CL2 PE=2 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 9.0e-229
Identity = 397/534 (74.34%), Postives = 455/534 (85.21%), Query Frame = 1

Query: 32  ENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVDLTTRKV 91
           + D+IFRSKLPDIYIPKHLPLH+YC  EN +K+G ++CLING TGE+FTY+ V+L +RKV
Sbjct: 8   KEDLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSRKV 67

Query: 92  ASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQAKGSKS 151
           ASGLNKLGI + D IMLLLPNSPE+ FAFLGASY GAI T ANPFFT+AE+ KQ K S +
Sbjct: 68  ASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLA 127

Query: 152 KLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDIDPEDVV 211
           KLIITQ+ Y +KVK+   E   ++I+ +D  P  CL F  L++ADE E+P V ID +DVV
Sbjct: 128 KLIITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVIDSDDVV 187

Query: 212 ALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHIYSLNSV 271
           ALPYSSGTTGLPKGVMLTHK LVTSVAQQVDG+NPNLY   EDV++C+LPLFHIYSLN+V
Sbjct: 188 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 247

Query: 272 LLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRI 331
           L CGLRAG TILIM KF+I   L+L +KY VT+ P VPPIVLAIAKSP ++KYDLSS+R 
Sbjct: 248 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRT 307

Query: 332 IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPGACGTVV 391
           + SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAK+P+ +K GACGTVV
Sbjct: 308 VMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVV 367

Query: 392 RNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFI 451
           RNAEMKIVD ET +SLPRN  GEICIRGDQIMKGYLN+PE+T  TID++GWLHTGDIGFI
Sbjct: 368 RNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFI 427

Query: 452 DDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVV 511
           DDDDE+FIVDRLKE+IKYKGFQVAPAELEALLLTHP ISDAAVVPM DE+AGEVPVAFVV
Sbjct: 428 DDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVV 487

Query: 512 KLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 566
           +      TE+EIKQF+SKQVVFYKRI RVFF+DAIPKSPSGKILRK+LRAK+A+
Sbjct: 488 RTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKIAS 539

BLAST of Cucsa.177420 vs. Swiss-Prot
Match: 4CL1_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum GN=4CL1 PE=2 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 3.4e-228
Identity = 395/534 (73.97%), Postives = 456/534 (85.39%), Query Frame = 1

Query: 32  ENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVDLTTRKV 91
           + D+IFRSKLPDIYIPKHLPLH+YC  EN +K+G ++CLING TGE+FTY+ V+L +RKV
Sbjct: 8   KEDLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSRKV 67

Query: 92  ASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQAKGSKS 151
           ASGLNKLGI + D IMLLLPNSPE+ FAFLGASY GAI T ANPFFT+AE+ KQ K S++
Sbjct: 68  ASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQA 127

Query: 152 KLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDIDPEDVV 211
           KLIITQ+ Y +KVK+   E   ++I+ +D  P  CL F  L++ADE E+P V I+ +DVV
Sbjct: 128 KLIITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVINSDDVV 187

Query: 212 ALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHIYSLNSV 271
           ALPYSSGTTGLPKGVMLTHK LVTSVAQQVDG+NPNLY   EDV++C+LPLFHIYSLN+V
Sbjct: 188 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 247

Query: 272 LLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRI 331
           L CGLRAG TILIM KF+I   L+L +KY VT+ P VPPIVLAIAKSP ++KYDLSS+R 
Sbjct: 248 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRT 307

Query: 332 IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPGACGTVV 391
           + SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAK+P+ +K GACGTVV
Sbjct: 308 VMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVV 367

Query: 392 RNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFI 451
           RNAEMKIVD ET +SLPRN  GEICIRGDQIMKGYLN+PE+T  TID++GWLHTGDIGFI
Sbjct: 368 RNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFI 427

Query: 452 DDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVV 511
           DDDDE+FIVDRLKE+IKYKGFQVAPAELEALLLTHP ISDAAVVPM DE+AGEVPVAFVV
Sbjct: 428 DDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVV 487

Query: 512 KLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 566
           +      TE+EIKQF+SKQVVFYKRI RVFF+DAIPKSPSGKILRK+LRA++A+
Sbjct: 488 RTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARIAS 539

BLAST of Cucsa.177420 vs. TrEMBL
Match: A0A0A0LF00_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G710200 PE=4 SV=1)

HSP 1 Score: 914.8 bits (2363), Expect = 5.1e-263
Identity = 463/465 (99.57%), Postives = 464/465 (99.78%), Query Frame = 1

Query: 107 MLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQAKGSKSKLIITQSSYYEKVKE 166
           MLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQAKGSKSKLIITQSSYYEKVKE
Sbjct: 1   MLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQAKGSKSKLIITQSSYYEKVKE 60

Query: 167 ITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDIDPEDVVALPYSSGTTGLPKGV 226
           ITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDIDPEDVVALPYSSGTTGLPKGV
Sbjct: 61  ITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDIDPEDVVALPYSSGTTGLPKGV 120

Query: 227 MLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHIYSLNSVLLCGLRAGSTILIMP 286
           MLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHIYSLNSVLLCGLRAGSTILIMP
Sbjct: 121 MLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHIYSLNSVLLCGLRAGSTILIMP 180

Query: 287 KFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRIIKSGGAPLGKELEDT 346
           KFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRIIKSGGAPLGKELEDT
Sbjct: 181 KFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRIIKSGGAPLGKELEDT 240

Query: 347 VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPGACGTVVRNAEMKIVDTETGSS 406
           VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPGACGTVVRNAEMKIVDTETGSS
Sbjct: 241 VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPGACGTVVRNAEMKIVDTETGSS 300

Query: 407 LPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDEMFIVDRLKEL 466
           LPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDEMFIVDRLKEL
Sbjct: 301 LPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDEMFIVDRLKEL 360

Query: 467 IKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEATEDEIKQF 526
           IKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEA+EDEIKQF
Sbjct: 361 IKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEASEDEIKQF 420

Query: 527 ISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAAFPNSN 572
           ISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAAFP SN
Sbjct: 421 ISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAAFPTSN 465

BLAST of Cucsa.177420 vs. TrEMBL
Match: D5LLN7_SORAU (p-coumarate:CoA ligase 1 OS=Sorbus aucuparia PE=2 SV=1)

HSP 1 Score: 844.7 bits (2181), Expect = 6.5e-242
Identity = 420/548 (76.64%), Postives = 479/548 (87.41%), Query Frame = 1

Query: 26  MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVD 85
           M IET+ ND+++RSKLPDI IPKHLPLHSYCL  N      + C+I+G TG+ +T+ DV+
Sbjct: 1   MAIETIPNDIVYRSKLPDIPIPKHLPLHSYCLH-NKNHSSSKPCIIDGATGDIYTFADVE 60

Query: 86  LTTRKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQ 145
           L  R+VASGLNKLGI + DVIMLLLPNSP F FAFLGAS+ GA+ TAANPFFT AEI KQ
Sbjct: 61  LNARRVASGLNKLGIQQGDVIMLLLPNSPAFAFAFLGASFRGAMTTAANPFFTPAEILKQ 120

Query: 146 AKGSKSKLIITQSSYYEKVKEIT---EELPEVKIMTVDSPPD-GCLWFGDLIKADEREVP 205
           AK SK+KLIIT + YY+KVK+++   +++ ++K+M VDSPPD  CL F +L++ADE ++P
Sbjct: 121 AKASKAKLIITLACYYDKVKDLSSSSDDVHDIKLMCVDSPPDPSCLHFSELLQADENDMP 180

Query: 206 RVDIDPEDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLP 265
            VDI P+DVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLYY  +DV+LCVLP
Sbjct: 181 EVDISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYSTDDVVLCVLP 240

Query: 266 LFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPEL 325
           LFHIYSLNSVLLCGLRAG+ IL+M KFEI  LL L +KY V++APIVPPIVLAIAK P+L
Sbjct: 241 LFHIYSLNSVLLCGLRAGAAILMMNKFEIVSLLGLIDKYKVSIAPIVPPIVLAIAKFPDL 300

Query: 326 EKYDLSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFP 385
           +KYDLSSIR++K GGAPLGKELEDTVRAKFP   LGQGYGMTEAGPVLTM LAFAK PF 
Sbjct: 301 DKYDLSSIRVLKCGGAPLGKELEDTVRAKFPNVTLGQGYGMTEAGPVLTMSLAFAKQPFE 360

Query: 386 MKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDG 445
           +KPG CGTVVRNAE+KIVD E+G+SLPRN PGEICIRGDQIMKGYLN+PE+T  TIDK+G
Sbjct: 361 VKPGGCGTVVRNAELKIVDPESGASLPRNQPGEICIRGDQIMKGYLNDPESTRTTIDKEG 420

Query: 446 WLHTGDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQ 505
           WLHTGDIGFIDDDDE+FIVDRLKELIKYKGFQVAPAELEALL+THP +SDAAVVPMKDE 
Sbjct: 421 WLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLITHPSVSDAAVVPMKDEA 480

Query: 506 AGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRA 565
           AGEVPVAFVV+  NS+ TEDE+KQFISKQVVFYKRINRVFFI+AIPKSPSGKILRK+LRA
Sbjct: 481 AGEVPVAFVVRSNNSQLTEDEVKQFISKQVVFYKRINRVFFIEAIPKSPSGKILRKDLRA 540

Query: 566 KLAAAFPN 570
           KLAA FPN
Sbjct: 541 KLAAGFPN 547

BLAST of Cucsa.177420 vs. TrEMBL
Match: M5X5B3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003747mg PE=4 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 1.9e-241
Identity = 419/552 (75.91%), Postives = 482/552 (87.32%), Query Frame = 1

Query: 26  MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVD 85
           M IET  ND+++RSKLPDI IPKHLPLHSY  Q+    +  + C+ING TG+ ++Y+DV+
Sbjct: 1   MAIETTPNDIVYRSKLPDIPIPKHLPLHSYVFQDKN-HLSSKPCIINGTTGDIYSYSDVE 60

Query: 86  LTTRKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQ 145
           L  R+VASGLNKLG+ + DVIMLLLPNSPEFVFAFLGAS+ GA+ T+ANPFFT AEI KQ
Sbjct: 61  LKARRVASGLNKLGVQQGDVIMLLLPNSPEFVFAFLGASFRGAMTTSANPFFTPAEILKQ 120

Query: 146 AKGSKSKLIITQSSYYEKVKEIT-------EELPEVKIMTVDSPPD-GCLWFGDLIKADE 205
           AK SK+KLIIT + YY+KVK+++       +++ ++K+M VDSPPD  CL F +L++ADE
Sbjct: 121 AKASKAKLIITLACYYDKVKDLSSSSSAGHDDVHDIKLMCVDSPPDPSCLHFSELLQADE 180

Query: 206 REVPRVDIDPEDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVIL 265
            ++P VDIDP DVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLYY  +DV+L
Sbjct: 181 NDMPEVDIDPNDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYSSDDVVL 240

Query: 266 CVLPLFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAK 325
           CVLPLFHIYSLNSVLLCGLRAG+ IL+M KFEI  LL L EK+ V++APIVPPIVLAIAK
Sbjct: 241 CVLPLFHIYSLNSVLLCGLRAGAAILMMNKFEIVSLLGLIEKHKVSIAPIVPPIVLAIAK 300

Query: 326 SPELEKYDLSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAK 385
            P+L+KYDLSSIR++K GGAPLGKELEDTVRAKFP   LGQGYGMTEAGPVLTM LAFAK
Sbjct: 301 FPDLDKYDLSSIRVLKCGGAPLGKELEDTVRAKFPNVTLGQGYGMTEAGPVLTMSLAFAK 360

Query: 386 DPFPMKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATI 445
            PF +KPG CGTVVRNA++KIVD ETG+SLPRN PGEICIRGDQIMKGYLN+PE+T ATI
Sbjct: 361 QPFGVKPGGCGTVVRNAQIKIVDPETGASLPRNQPGEICIRGDQIMKGYLNDPESTRATI 420

Query: 446 DKDGWLHTGDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPM 505
           DK+GWLHTGDIGFIDDD+E+FIVDRLKELIKYKGFQVAPAELEALL+THP +SDAAVVPM
Sbjct: 421 DKEGWLHTGDIGFIDDDEELFIVDRLKELIKYKGFQVAPAELEALLVTHPSVSDAAVVPM 480

Query: 506 KDEQAGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRK 565
           KDE AGEVPVAFVV+ KN++ TEDEIKQFISKQVVFYKR+NRVFFI+AIPKSPSGKILRK
Sbjct: 481 KDEAAGEVPVAFVVRSKNAQITEDEIKQFISKQVVFYKRLNRVFFIEAIPKSPSGKILRK 540

Query: 566 ELRAKLAAAFPN 570
           +LRAKLA  FPN
Sbjct: 541 DLRAKLATGFPN 551

BLAST of Cucsa.177420 vs. TrEMBL
Match: A0A089YQ16_BETPN (4-coumarate:CoA ligase 1 OS=Betula pendula PE=2 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 2.5e-241
Identity = 417/544 (76.65%), Postives = 475/544 (87.32%), Query Frame = 1

Query: 26  MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVD 85
           M I+  E + IFRSKLPDIYIPKHLP+HSYC  EN +K+G R CLING+TG+ +TY DV+
Sbjct: 1   MAIQAKEQEFIFRSKLPDIYIPKHLPIHSYCF-ENISKVGSRPCLINGLTGKVYTYYDVE 60

Query: 86  LTTRKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQ 145
           LT RKVASGL+KLGI K DV+MLLLPNSPEF F FLGASYLGA+ TAANPF TA E+ KQ
Sbjct: 61  LTARKVASGLSKLGIQKGDVVMLLLPNSPEFAFVFLGASYLGAMTTAANPFCTAGEVSKQ 120

Query: 146 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 205
           AK + +K+++TQ+ YY++VK+ T E   VKI+ +DSPP+ CL F +L KADE +V  VDI
Sbjct: 121 AKSANAKIVVTQACYYDRVKDYTNE-NGVKIICIDSPPEDCLHFSELTKADENDVAEVDI 180

Query: 206 DPEDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 265
            P+DVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLYY  EDVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHSEDVILCVLPLFHI 240

Query: 266 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 325
           YSLNSV LCGLRAG++ILI+PKFEI  LLQL +K+ VTV PIVPPIVLAI K P+L+KYD
Sbjct: 241 YSLNSVFLCGLRAGASILILPKFEIVSLLQLIQKHKVTVMPIVPPIVLAITKFPDLDKYD 300

Query: 326 LSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 385
           LSS++++KSGGAPLGKE+E+TV+AKFP A+ GQGYGMTEAGPVL M LAFAK+P  +K G
Sbjct: 301 LSSVKMLKSGGAPLGKEIEETVKAKFPNALFGQGYGMTEAGPVLAMCLAFAKEPMEVKSG 360

Query: 386 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 445
           ACGTVVRNAEMKIVD ETG+SLPRN PGEICIRGDQIMKGY+N+PEATA+TIDK+GWLHT
Sbjct: 361 ACGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYINDPEATASTIDKEGWLHT 420

Query: 446 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 505
           GDIG IDD+DE+FIVDRLKELIKYKGFQVAPAELEALLLTHP ISDAAVVPMKD+ AGEV
Sbjct: 421 GDIGLIDDNDELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVPMKDDLAGEV 480

Query: 506 PVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 565
           PVAFV +   S+ TEDEIKQF+SKQVVFYKRI+RVFFID +PKSPSGKILRKELRAKLAA
Sbjct: 481 PVAFVARSNGSQVTEDEIKQFVSKQVVFYKRISRVFFIDVVPKSPSGKILRKELRAKLAA 540

Query: 566 AFPN 570
            F N
Sbjct: 541 GFSN 542

BLAST of Cucsa.177420 vs. TrEMBL
Match: Q5S017_BETPL (4-coumarate:CoA ligase OS=Betula platyphylla PE=2 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 2.5e-241
Identity = 417/544 (76.65%), Postives = 475/544 (87.32%), Query Frame = 1

Query: 26  MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVD 85
           M I+  E + IFRSKLPDIYIPKHLP+HSYC  EN +K+G R CLING+TG+ +TY DV+
Sbjct: 1   MAIQAKEQEFIFRSKLPDIYIPKHLPIHSYCF-ENISKVGSRPCLINGLTGKVYTYYDVE 60

Query: 86  LTTRKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQ 145
           LT RKVASGL+KLGI K DV+MLLLPNSPEF F FLGASYLGA+ TAANPF TA E+ KQ
Sbjct: 61  LTARKVASGLSKLGIQKGDVVMLLLPNSPEFAFVFLGASYLGAMTTAANPFCTAGEVSKQ 120

Query: 146 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 205
           AK + +K+++TQ+ YY++VK+ T E   VKI+ +DSPP+ CL F +L KADE +V  VDI
Sbjct: 121 AKSANAKIVVTQACYYDRVKDYTNE-NGVKIICIDSPPEDCLHFSELTKADENDVAEVDI 180

Query: 206 DPEDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 265
            P+DVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLYY  EDVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHSEDVILCVLPLFHI 240

Query: 266 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 325
           YSLNSV LCGLRAG++ILI+PKFEI  LLQL +K+ VTV PIVPPIVLAI K P+L+KYD
Sbjct: 241 YSLNSVFLCGLRAGASILILPKFEIVSLLQLIQKHKVTVMPIVPPIVLAITKFPDLDKYD 300

Query: 326 LSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 385
           LSS++++KSGGAPLGKE+E+TV+AKFP A+ GQGYGMTEAGPVL M LAFAK+P  +K G
Sbjct: 301 LSSVKMLKSGGAPLGKEIEETVKAKFPNALFGQGYGMTEAGPVLAMCLAFAKEPMEVKSG 360

Query: 386 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 445
           ACGTVVRNAEMKIVD ETG+SLPRN PGEICIRGDQIMKGY+N+PEATA+TIDK+GWLHT
Sbjct: 361 ACGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYINDPEATASTIDKEGWLHT 420

Query: 446 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 505
           GDIG IDD+DE+FIVDRLKELIKYKGFQVAPAELEALLLTHP ISDAAVVPMKD+ AGEV
Sbjct: 421 GDIGLIDDNDELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVPMKDDLAGEV 480

Query: 506 PVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 565
           PVAFV +   S+ TEDEIKQF+SKQVVFYKRI+RVFFID +PKSPSGKILRKELRAKLAA
Sbjct: 481 PVAFVARSNGSQVTEDEIKQFVSKQVVFYKRISRVFFIDVVPKSPSGKILRKELRAKLAA 540

Query: 566 AFPN 570
            F N
Sbjct: 541 GFSN 542

BLAST of Cucsa.177420 vs. TAIR10
Match: AT3G21240.1 (AT3G21240.1 4-coumarate:CoA ligase 2)

HSP 1 Score: 770.4 bits (1988), Expect = 7.8e-223
Identity = 387/544 (71.14%), Postives = 454/544 (83.46%), Query Frame = 1

Query: 33  NDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVDLTTRKVA 92
           NDVIFRS+LPDIYIP HLPLH Y   EN ++   + CLING TGE +TY DV +T+RK+A
Sbjct: 19  NDVIFRSRLPDIYIPNHLPLHDYIF-ENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 78

Query: 93  SGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQAKGSKSK 152
           +GL+ LG+ + DV+M+LLPNSPE V  FL AS++GAI T+ANPFFT AEI KQAK S +K
Sbjct: 79  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 138

Query: 153 LIITQSSYYEKVKEITEELPEVKIMTVDSP--PDGCLWFGDLIKADEREVPRVD-----I 212
           LI+TQS Y +K+K +  +   V I+T DS   P+ CL F +L +++E   PRVD     I
Sbjct: 139 LIVTQSRYVDKIKNLQND--GVLIVTTDSDAIPENCLRFSELTQSEE---PRVDSIPEKI 198

Query: 213 DPEDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 272
            PEDVVALP+SSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+  +DVILCVLP+FHI
Sbjct: 199 SPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHI 258

Query: 273 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 332
           Y+LNS++LC LR G+TILIMPKFEI LLL+  ++  VTVA +VPPIVLAIAKSPE EKYD
Sbjct: 259 YALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYD 318

Query: 333 LSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 392
           LSS+R++KSG APLGKELED + AKFP A LGQGYGMTEAGPVL M L FAK+PFP+K G
Sbjct: 319 LSSVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSG 378

Query: 393 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 452
           ACGTVVRNAEMKI+D +TG SLPRN PGEICIRG+QIMKGYLN+P ATA+TIDKDGWLHT
Sbjct: 379 ACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHT 438

Query: 453 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 512
           GD+GFIDDDDE+FIVDRLKELIKYKGFQVAPAELE+LL+ HP I+D AVV MK+E AGEV
Sbjct: 439 GDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEV 498

Query: 513 PVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 570
           PVAFVV+ K+S  +EDEIKQF+SKQVVFYKRIN+VFF D+IPK+PSGKILRK+LRA+LA 
Sbjct: 499 PVAFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLAN 556

BLAST of Cucsa.177420 vs. TAIR10
Match: AT1G51680.1 (AT1G51680.1 4-coumarate:CoA ligase 1)

HSP 1 Score: 755.0 bits (1948), Expect = 3.4e-218
Identity = 380/539 (70.50%), Postives = 443/539 (82.19%), Query Frame = 1

Query: 33  NDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVDLTTRKVA 92
           +DVIFRSKLPDIYIP HL LH Y  Q N ++   + CLING TG  +TY+DV + +R++A
Sbjct: 21  SDVIFRSKLPDIYIPNHLSLHDYIFQ-NISEFATKPCLINGPTGHVYTYSDVHVISRQIA 80

Query: 93  SGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQAKGSKSK 152
           +  +KLG+ + DV+MLLLPN PEFV +FL AS+ GA  TAANPFFT AEI KQAK S +K
Sbjct: 81  ANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTK 140

Query: 153 LIITQSSYYEKVKEITEELPEVKIMTVDSP----PDGCLWFGDLIKADERE---VPRVDI 212
           LIIT++ Y +K+K +  +   V +   D+     P+GCL F +L ++       +  V+I
Sbjct: 141 LIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEI 200

Query: 213 DPEDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 272
            P+DVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+  +DVILCVLP+FHI
Sbjct: 201 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHI 260

Query: 273 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 332
           Y+LNS++LCGLR G+ ILIMPKFEI LLL+L ++  VTVAP+VPPIVLAIAKS E EKYD
Sbjct: 261 YALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYD 320

Query: 333 LSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 392
           LSSIR++KSG APLGKELED V AKFP A LGQGYGMTEAGPVL M L FAK+PFP+K G
Sbjct: 321 LSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSG 380

Query: 393 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 452
           ACGTVVRNAEMKIVD +TG SL RN PGEICIRG QIMKGYLNNP ATA TIDKDGWLHT
Sbjct: 381 ACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHT 440

Query: 453 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 512
           GDIG IDDDDE+FIVDRLKELIKYKGFQVAPAELEALL+ HP I+D AVV MK+E AGEV
Sbjct: 441 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEV 500

Query: 513 PVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 565
           PVAFVVK K+SE +ED++KQF+SKQVVFYKRIN+VFF ++IPK+PSGKILRK+LRAKLA
Sbjct: 501 PVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLA 558

BLAST of Cucsa.177420 vs. TAIR10
Match: AT1G65060.1 (AT1G65060.1 4-coumarate:CoA ligase 3)

HSP 1 Score: 683.3 bits (1762), Expect = 1.3e-196
Identity = 340/531 (64.03%), Postives = 425/531 (80.04%), Query Frame = 1

Query: 36  IFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVDLTTRKVASGL 95
           IFRSKLPDI IP HLPLH+YC  E  + +  + CLI G TG+S+TY +  L  R+VASGL
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCF-EKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGL 93

Query: 96  NKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQAKGSKSKLII 155
            KLGI K DVIM+LL NS EFVF+F+GAS +GA+ T ANPF+T+ E+ KQ K S +KLII
Sbjct: 94  YKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLII 153

Query: 156 TQSSYYEKVKEITEELPEVKIMTVDSP-PDGCLWFGDLIKADEREV--PRVDIDPEDVVA 215
           T S Y +K+K + E L    ++T D P P+ CL F  LI  DE       VDI  +D  A
Sbjct: 154 THSQYVDKLKNLGENLT---LITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAA 213

Query: 216 LPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHIYSLNSVL 275
           LP+SSGTTGLPKGV+LTHKSL+TSVAQQVDG+NPNLY    DVILCVLPLFHIYSLNSVL
Sbjct: 214 LPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVL 273

Query: 276 LCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRII 335
           L  LR+G+T+L+M KFEIG LL L +++ VT+A +VPP+V+A+AK+P +  YDLSS+R +
Sbjct: 274 LNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFV 333

Query: 336 KSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPGACGTVVR 395
            SG APLGKEL+D++R + P+A+LGQGYGMTEAGPVL+M L FAK+P P K G+CGTVVR
Sbjct: 334 LSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVR 393

Query: 396 NAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFID 455
           NAE+K+V  ET  SL  N PGEICIRG QIMK YLN+PEAT+ATID++GWLHTGDIG++D
Sbjct: 394 NAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVD 453

Query: 456 DDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVK 515
           +DDE+FIVDRLKE+IK+KGFQV PAELE+LL+ H  I+DAAVVP  DE AGEVPVAFVV+
Sbjct: 454 EDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVR 513

Query: 516 LKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKL 564
              ++ TE+++K++++KQVVFYKR+++VFF+ +IPKSPSGKILRK+L+AKL
Sbjct: 514 SNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of Cucsa.177420 vs. TAIR10
Match: AT3G21230.1 (AT3G21230.1 4-coumarate:CoA ligase 5)

HSP 1 Score: 671.0 bits (1730), Expect = 6.5e-193
Identity = 333/545 (61.10%), Postives = 415/545 (76.15%), Query Frame = 1

Query: 29  ETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAK---IGHRTCLINGVTGESFTYNDVD 88
           E   +D IFRSKLPDI+IP HLPL  Y  Q  +         TC+I+G TG   TY DV 
Sbjct: 21  EEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADVQ 80

Query: 89  LTTRKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQ 148
              R++A+G+++LGI   DV+MLLLPNSPEF  +FL  +YLGA+ T ANPF+T  EI KQ
Sbjct: 81  TNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQ 140

Query: 149 AKGSKSKLIITQSSYYEKVKEITEELPEVKIM-------TVDSPPDGCLWFGDLIKADER 208
           AK S +K+IIT+    +K+  +  +   +  +        V S  DGC+ F +L +ADE 
Sbjct: 141 AKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADET 200

Query: 209 EVPRVDIDPEDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILC 268
           E+ +  I PED VA+PYSSGTTGLPKGVM+THK LVTS+AQ+VDGENPNL +   DVILC
Sbjct: 201 ELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILC 260

Query: 269 VLPLFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKS 328
            LP+FHIY+L++++L  +R G+ +LI+P+FE+ L+++L ++Y VTV P+ PP+VLA  KS
Sbjct: 261 FLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIKS 320

Query: 329 PELEKYDLSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKD 388
           PE E+YDLSS+RI+ SG A L KELED VR KFP A+ GQGYGMTE+G V    LAFAK+
Sbjct: 321 PETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAKN 380

Query: 389 PFPMKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATID 448
           PF  K GACGTV+RNAEMK+VDTETG SLPRN  GEIC+RG Q+MKGYLN+PEATA TID
Sbjct: 381 PFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTID 440

Query: 449 KDGWLHTGDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMK 508
           KDGWLHTGDIGF+DDDDE+FIVDRLKELIK+KG+QVAPAELEALL++HP I DAAVV MK
Sbjct: 441 KDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAMK 500

Query: 509 DEQAGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKE 564
           DE A EVPVAFV + + S+ TED++K +++KQVV YKRI  VFFI+ IPK+ SGKILRK+
Sbjct: 501 DEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRKD 560

BLAST of Cucsa.177420 vs. TAIR10
Match: AT4G05160.1 (AT4G05160.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 424.9 bits (1091), Expect = 8.1e-119
Identity = 239/543 (44.01%), Postives = 341/543 (62.80%), Query Frame = 1

Query: 34  DVIFRSKLPDIYIPK--HLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVDLTTRKV 93
           D I+RS  P + +PK  +  L S+ L  N++    +  + +  TG+S T++ +     ++
Sbjct: 9   DGIYRSLRPTLVLPKDPNTSLVSF-LFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARL 68

Query: 94  ASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQAKGSKS 153
           A G ++LGI K DV+++  PNS +F   FL  + +G + T ANP +T  E+ KQ K S  
Sbjct: 69  AHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNP 128

Query: 154 KLIITQSSYYEKVKEITEELPEVKIM---TVDSPPDG---CLWFGDLIKADE--REVPRV 213
           K+II+ +  ++K+K    +LP V +    TV+ PP      L F ++++  E   E P V
Sbjct: 129 KIIISVNQLFDKIKGF--DLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVSEYPFV 188

Query: 214 DIDPEDVVALPYSSGTTGLPKGVMLTH-----KSLVTSVAQQVDGENPNLYYGHEDVILC 273
           +I   D  AL YSSGTTG  KGV LTH      SL+ ++ Q + GE    Y+G   V LC
Sbjct: 189 EIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGE----YHG---VFLC 248

Query: 274 VLPLFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKS 333
            LP+FH++ L  +    L+ G+ ++ M +FE+ L+L+  EK+ VT   +VPP+ LA++K 
Sbjct: 249 FLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQ 308

Query: 334 PELEKYDLSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKD 393
             ++K+DLSS++ I SG APLGK+L +      P  +L QGYGMTE     T G+   +D
Sbjct: 309 SIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTE-----TCGIVSVED 368

Query: 394 PFPMK--PGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAAT 453
           P   K   G+ G +    E +IV  ETG S P N  GEI +RG  +MKGYLNNP+AT  T
Sbjct: 369 PRLGKRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKET 428

Query: 454 IDKDGWLHTGDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVP 513
           IDK  W+HTGD+G+ ++D  +++VDR+KELIKYKGFQVAPAELE LL++HP I DA V+P
Sbjct: 429 IDKKSWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIP 488

Query: 514 MKDEQAGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILR 560
             DE+AGEVP+AFVV+  NS  TE +I++FI+KQV  YKR+ RV FI  +PKS +GKILR
Sbjct: 489 FPDEEAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILR 536

BLAST of Cucsa.177420 vs. NCBI nr
Match: gi|778682904|ref|XP_004137646.2| (PREDICTED: 4-coumarate--CoA ligase 1 [Cucumis sativus])

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 544/546 (99.63%), Postives = 545/546 (99.82%), Query Frame = 1

Query: 26  MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVD 85
           MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVD 60

Query: 86  LTTRKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQ 145
           LTTRKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQ
Sbjct: 61  LTTRKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQ 120

Query: 146 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 205
           AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI
Sbjct: 121 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 180

Query: 206 DPEDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 265
           DPEDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI
Sbjct: 181 DPEDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 240

Query: 266 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 325
           YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 326 LSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 385
           LSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG
Sbjct: 301 LSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 360

Query: 386 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 445
           ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 446 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 505
           GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 506 PVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 565
           PVAFVVKLKNSEA+EDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA
Sbjct: 481 PVAFVVKLKNSEASEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 540

Query: 566 AFPNSN 572
           AFP SN
Sbjct: 541 AFPTSN 546

BLAST of Cucsa.177420 vs. NCBI nr
Match: gi|659125990|ref|XP_008462955.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo])

HSP 1 Score: 978.4 bits (2528), Expect = 5.4e-282
Identity = 493/519 (94.99%), Postives = 508/519 (97.88%), Query Frame = 1

Query: 26  MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVD 85
           MGIETVENDVIFRSKLPDIYIP HLPLHSYCLQENAAKIGHRTCLINGVTGESFT+NDVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60

Query: 86  LTTRKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQ 145
           LT RKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGAS+LGAIMTAANPFFTAAEI KQ
Sbjct: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120

Query: 146 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 205
           AKGSK+KLIITQSSYYEK+KEITEELPEVKIMTVDSP DGCLWFGDLIKADERE+PRV+I
Sbjct: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180

Query: 206 DPEDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 265
           DP+DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYG+EDVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240

Query: 266 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 325
           YSLNSVLLCGLRAGSTILIMPKFEIGLLLQL EKYGVTVAPIVPPIVLAIAKSPELEKYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 326 LSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 385
           LSSIR+IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAK+PFPMKPG
Sbjct: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 386 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 445
           ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 446 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 505
           GDIGFID+DDE+FIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 506 PVAFVVKLKNSEATEDEIKQFISKQV-VFY--KRINRVF 542
           PVAFVVKLKNSEATEDEIKQFISKQV VF+    +N +F
Sbjct: 481 PVAFVVKLKNSEATEDEIKQFISKQVSVFFYLTNLNSIF 519

BLAST of Cucsa.177420 vs. NCBI nr
Match: gi|700203508|gb|KGN58641.1| (hypothetical protein Csa_3G710200 [Cucumis sativus])

HSP 1 Score: 914.8 bits (2363), Expect = 7.3e-263
Identity = 463/465 (99.57%), Postives = 464/465 (99.78%), Query Frame = 1

Query: 107 MLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQAKGSKSKLIITQSSYYEKVKE 166
           MLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQAKGSKSKLIITQSSYYEKVKE
Sbjct: 1   MLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQAKGSKSKLIITQSSYYEKVKE 60

Query: 167 ITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDIDPEDVVALPYSSGTTGLPKGV 226
           ITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDIDPEDVVALPYSSGTTGLPKGV
Sbjct: 61  ITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDIDPEDVVALPYSSGTTGLPKGV 120

Query: 227 MLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHIYSLNSVLLCGLRAGSTILIMP 286
           MLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHIYSLNSVLLCGLRAGSTILIMP
Sbjct: 121 MLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHIYSLNSVLLCGLRAGSTILIMP 180

Query: 287 KFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRIIKSGGAPLGKELEDT 346
           KFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRIIKSGGAPLGKELEDT
Sbjct: 181 KFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRIIKSGGAPLGKELEDT 240

Query: 347 VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPGACGTVVRNAEMKIVDTETGSS 406
           VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPGACGTVVRNAEMKIVDTETGSS
Sbjct: 241 VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPGACGTVVRNAEMKIVDTETGSS 300

Query: 407 LPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDEMFIVDRLKEL 466
           LPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDEMFIVDRLKEL
Sbjct: 301 LPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDEMFIVDRLKEL 360

Query: 467 IKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEATEDEIKQF 526
           IKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEA+EDEIKQF
Sbjct: 361 IKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEASEDEIKQF 420

Query: 527 ISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAAFPNSN 572
           ISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAAFP SN
Sbjct: 421 ISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAAFPTSN 465

BLAST of Cucsa.177420 vs. NCBI nr
Match: gi|294714021|gb|ADF30254.1| (p-coumarate:CoA ligase 1 [Sorbus aucuparia])

HSP 1 Score: 844.7 bits (2181), Expect = 9.3e-242
Identity = 420/548 (76.64%), Postives = 479/548 (87.41%), Query Frame = 1

Query: 26  MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVD 85
           M IET+ ND+++RSKLPDI IPKHLPLHSYCL  N      + C+I+G TG+ +T+ DV+
Sbjct: 1   MAIETIPNDIVYRSKLPDIPIPKHLPLHSYCLH-NKNHSSSKPCIIDGATGDIYTFADVE 60

Query: 86  LTTRKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQ 145
           L  R+VASGLNKLGI + DVIMLLLPNSP F FAFLGAS+ GA+ TAANPFFT AEI KQ
Sbjct: 61  LNARRVASGLNKLGIQQGDVIMLLLPNSPAFAFAFLGASFRGAMTTAANPFFTPAEILKQ 120

Query: 146 AKGSKSKLIITQSSYYEKVKEIT---EELPEVKIMTVDSPPD-GCLWFGDLIKADEREVP 205
           AK SK+KLIIT + YY+KVK+++   +++ ++K+M VDSPPD  CL F +L++ADE ++P
Sbjct: 121 AKASKAKLIITLACYYDKVKDLSSSSDDVHDIKLMCVDSPPDPSCLHFSELLQADENDMP 180

Query: 206 RVDIDPEDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLP 265
            VDI P+DVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLYY  +DV+LCVLP
Sbjct: 181 EVDISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYSTDDVVLCVLP 240

Query: 266 LFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPEL 325
           LFHIYSLNSVLLCGLRAG+ IL+M KFEI  LL L +KY V++APIVPPIVLAIAK P+L
Sbjct: 241 LFHIYSLNSVLLCGLRAGAAILMMNKFEIVSLLGLIDKYKVSIAPIVPPIVLAIAKFPDL 300

Query: 326 EKYDLSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFP 385
           +KYDLSSIR++K GGAPLGKELEDTVRAKFP   LGQGYGMTEAGPVLTM LAFAK PF 
Sbjct: 301 DKYDLSSIRVLKCGGAPLGKELEDTVRAKFPNVTLGQGYGMTEAGPVLTMSLAFAKQPFE 360

Query: 386 MKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDG 445
           +KPG CGTVVRNAE+KIVD E+G+SLPRN PGEICIRGDQIMKGYLN+PE+T  TIDK+G
Sbjct: 361 VKPGGCGTVVRNAELKIVDPESGASLPRNQPGEICIRGDQIMKGYLNDPESTRTTIDKEG 420

Query: 446 WLHTGDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQ 505
           WLHTGDIGFIDDDDE+FIVDRLKELIKYKGFQVAPAELEALL+THP +SDAAVVPMKDE 
Sbjct: 421 WLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLITHPSVSDAAVVPMKDEA 480

Query: 506 AGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRA 565
           AGEVPVAFVV+  NS+ TEDE+KQFISKQVVFYKRINRVFFI+AIPKSPSGKILRK+LRA
Sbjct: 481 AGEVPVAFVVRSNNSQLTEDEVKQFISKQVVFYKRINRVFFIEAIPKSPSGKILRKDLRA 540

Query: 566 KLAAAFPN 570
           KLAA FPN
Sbjct: 541 KLAAGFPN 547

BLAST of Cucsa.177420 vs. NCBI nr
Match: gi|595935993|ref|XP_007215519.1| (hypothetical protein PRUPE_ppa003747mg [Prunus persica])

HSP 1 Score: 843.2 bits (2177), Expect = 2.7e-241
Identity = 419/552 (75.91%), Postives = 482/552 (87.32%), Query Frame = 1

Query: 26  MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVD 85
           M IET  ND+++RSKLPDI IPKHLPLHSY  Q+    +  + C+ING TG+ ++Y+DV+
Sbjct: 1   MAIETTPNDIVYRSKLPDIPIPKHLPLHSYVFQDKN-HLSSKPCIINGTTGDIYSYSDVE 60

Query: 86  LTTRKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQ 145
           L  R+VASGLNKLG+ + DVIMLLLPNSPEFVFAFLGAS+ GA+ T+ANPFFT AEI KQ
Sbjct: 61  LKARRVASGLNKLGVQQGDVIMLLLPNSPEFVFAFLGASFRGAMTTSANPFFTPAEILKQ 120

Query: 146 AKGSKSKLIITQSSYYEKVKEIT-------EELPEVKIMTVDSPPD-GCLWFGDLIKADE 205
           AK SK+KLIIT + YY+KVK+++       +++ ++K+M VDSPPD  CL F +L++ADE
Sbjct: 121 AKASKAKLIITLACYYDKVKDLSSSSSAGHDDVHDIKLMCVDSPPDPSCLHFSELLQADE 180

Query: 206 REVPRVDIDPEDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVIL 265
            ++P VDIDP DVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLYY  +DV+L
Sbjct: 181 NDMPEVDIDPNDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYSSDDVVL 240

Query: 266 CVLPLFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAK 325
           CVLPLFHIYSLNSVLLCGLRAG+ IL+M KFEI  LL L EK+ V++APIVPPIVLAIAK
Sbjct: 241 CVLPLFHIYSLNSVLLCGLRAGAAILMMNKFEIVSLLGLIEKHKVSIAPIVPPIVLAIAK 300

Query: 326 SPELEKYDLSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAK 385
            P+L+KYDLSSIR++K GGAPLGKELEDTVRAKFP   LGQGYGMTEAGPVLTM LAFAK
Sbjct: 301 FPDLDKYDLSSIRVLKCGGAPLGKELEDTVRAKFPNVTLGQGYGMTEAGPVLTMSLAFAK 360

Query: 386 DPFPMKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATI 445
            PF +KPG CGTVVRNA++KIVD ETG+SLPRN PGEICIRGDQIMKGYLN+PE+T ATI
Sbjct: 361 QPFGVKPGGCGTVVRNAQIKIVDPETGASLPRNQPGEICIRGDQIMKGYLNDPESTRATI 420

Query: 446 DKDGWLHTGDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPM 505
           DK+GWLHTGDIGFIDDD+E+FIVDRLKELIKYKGFQVAPAELEALL+THP +SDAAVVPM
Sbjct: 421 DKEGWLHTGDIGFIDDDEELFIVDRLKELIKYKGFQVAPAELEALLVTHPSVSDAAVVPM 480

Query: 506 KDEQAGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRK 565
           KDE AGEVPVAFVV+ KN++ TEDEIKQFISKQVVFYKR+NRVFFI+AIPKSPSGKILRK
Sbjct: 481 KDEAAGEVPVAFVVRSKNAQITEDEIKQFISKQVVFYKRLNRVFFIEAIPKSPSGKILRK 540

Query: 566 ELRAKLAAAFPN 570
           +LRAKLA  FPN
Sbjct: 541 DLRAKLATGFPN 551