Cucsa.167500 (gene) Cucumber (Gy14) v1

NameCucsa.167500
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionWRKY protein
Locationscaffold01154 : 862999 .. 866145 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACAATAACAATTATCAGCAAGCTGGTGATTTGACTGATGTGATCCGACTGAGTTCCGCCCCTGTCGCCGGTCATTTCTCCTCCGAATTCTCTCTCGACCCGTTTTCCGGTGACAGACAACTCTGGTCGCACCTTCCTGCTGACAATTCTTCCATGAATTTTGGAGATCCGTTGTCGTTTCCAACTCGAGATCCATTTCTTCTTCCCCATTTTTCTCCCTTCAACTTTGCTTCGGATGGCGGCGGTGAGGGTTTGGCGGCGGACGACCAGCCGTCTAATTTACTTTCCCATATGTTGCAGATCTCTCCGAGTTCTGGTGATGGTGTAATTAGTACTCCTCCATGTGAGTCGCTCGCCACAGCAGTGGGGAATTCTCCGAGGTCGACTTCGGTTCGTGGTGGCGGTGGTCTTGTGCTTCCGACCGGTGGCTCGAACTCTCTTTGTTTAATGGAGAATTCCGGGATTCAGATCTCTTCTCCGCGAAATTCCGCCAATAAAAGAAGGTAAAATCAAATCTTGGACTTCTGAAATGATAATAATAACTCAGTATATATTATGTTAATGGGTGCTTTTGATCATTCTGAGTTATAACTTGTTGGGGTTCAAATGAAATTtAAATTTTGTCTTACCTTTGAGTTTCGAAAGCTTCATGATAGTTGATTAATTAGGTACTTAAAAAAAaTTATGGGTTTTAGAAAAaTATGTATAGGCTTTCTCCTATAGGGATTATTTTAGGATATTGCTGGGTTCAAACTCTCTAGTCCAACAAATGGAAAATAGGAGCATAAAGCTATGACTTTTAAGACATAATTCTCTTATGACTGAATGAACTTTGTCTTTACTATATTTATAGAAGGTTCAAAGGGTGGAGAAAACATTTTTTtCCCTTAAATATACCTTGGAGAAATGTAAACTCTCGTAGTCTTCGATCACATTTCAATGTCTACTTTAATGAAAAAATAAAACTAAAAGGAAAACAAATCAAATTTAGACTATGTTCTAAATAAAATAACAGTCACTAGCTATTTCAAGACCATATACATATCTATGTACAGACTCTATCTCATTTTTAGAGTTTAAGTTAATAAACTATATTAAGATTGCTGTAGATAAATTAGCATTAAAATTTTAAATATTTGGTAGCTCTAATTAACCACAAGTTTAATTAGAGTGGAATAACTAAGAAAAATTCTACCGCCTCTCCCATAGTTAACTAAAACTCCATCTGATCATAACAATTTAACTTTCCTTTtGTTTTtATTAAAAAAAAaaGAAGAAGAAGAAATAAAGAAGAGAAAAaCAAGAGAATTGAACTCCAAATTACAAAATTGTTGCTTCAAACAAAAACGTTACTAAAATGAATTCCTACATGAATGAATTAACTAATGTAGTTGGTTGTTCTCAGGAAGAGCCAAGTGAAGAAGGTGGTGTGTATTCCGGCACCGGCACCAGCGAACAGTCGATCAAGCAGCGGCGAAGTCGTTCCTTCTGATCTATGGGCATGGAGGAAGTACGGACAAAAGCCCATCAAAGGATCCCCATATCCAAGGTCAACTTTCTTCAAAACTTTAATCTTATTATTACTTTTAGGTTAAATATATTATCATTTGGTTCCCTCTCTTCCTTCCATAAATCCTAAATTTAGTCCTCAAATTGTTTAAATAATATATAACAACAATAATAACATTCATGAAATAATTATATATAATAGGAGAGTGTATTCCTAAATTTATAACAACAAaAAAAAAaGTTGCTAATAGATAATAAAACGTCTTTAAAAAAATCAACTAAATGAACAAACAAGAGAAGTATGTTAAGATTTCTGCAAGACACTATATAAAATTCAAAATGTGAAAGTGTTGGTAGATTAAGTTGAATTGAAATGATTTTAACTGTCTAAATTGATCATTGTTCGTCCTAACTTTCCAAGTCAAAAAGATTTTGACCTCAAATATTAATTTTTTTTtCTAATTTTCTTTGGTAATTTTCAATTAATTTATTTTCTACTTAAACATGAGAGAGAGAAAAAAAaGAGTTTTGTTTTCCAGAAATCAATGCCAAATGCTGCAAGTTTTTGTACCTCTTTCTCTCTCTCTCTAGCTGACAAGAAATTGACTCCCAATAATTTCATGAATATCTCACTCATGATCATTTACTCATCATTTCTTCAAGTGGGCTTTAAAGTTGGGGCAGAGAAATCATAATATTTTCTAAACTTCTATATATACTAATATATTTTTTATTTATAGAAATACTATATAGTTTTGAGATGTTCTTTATTTCAATTTATGGTATTTAATTTGTTCTAGTCCAATTCTTTAGTTGTTGGTGTTAATCAAAACATGTGTTTTGTGTTCCATTTGTATTTTTCTTCCCTTATTAATGATGGTTGACTTTTCAAAATTAAATGTTATTAGTAGATAAATGGTAATTCTTTTTTTtCCTTTTAATTACTTTGGATGTGATTTTTAAAAaCTAACTAGCTTGTTTAACAATTCCTTTCTTATGTTGTAGAAGTATTTACATTTTTtCCtAAAACCCTCAAATCTTTCATATACTTAATTCTCATTAATGTTGATCATCTTCACTATATATATATAGTATGTGCCTTATAGTTATTTTATGCCTTTCTTTCTCTTCTCTTTTCTTTCAGGGGATATTATAGGTGCAGTAGTTCCAAGGGCTGCTCCGCTCGAAAGCAGGTCGAGCGAAGTCGTACTAACCCCAACATGCTTGTCATCACCTACACTTCGGAGCACAACCACCCATGGCCCACTCAACGCAATGCCCTTGCAGGTTCTACTCGTTCCCATCCTTCACGAACCACCACCGCTACCACCGCTAATAAAACAAGCCCGAAACATGAGCGCATTAACGACATCCTGCCAACCACAACAATAAAGGAGGAAGAGATGGATGAAGACCAAATGACAGATAAAGCAACAACAACAAATGATAATCAGGAGGAGGGAGATCATCAAGATTTCCCTTATGACTTAATTTTCACTGAATTTGCAGATCAAATGAACGACCTCGAGCACGACAACAACCACGACCATAATCATCATCTTTCAGCAGACCCTCTGAACAACAATATGTTGATGTTTAGTAACCATGGATTTAGTGCAGGAAATGGAGAAGGCA

mRNA sequence

ATGGACAATAACAATTATCAGCAAGCTGGTGATTTGACTGATGTGATCCGACTGAGTTCCGCCCCTGTCGCCGGTCATTTCTCCTCCGAATTCTCTCTCGACCCGTTTTCCGGTGACAGACAACTCTGGTCGCACCTTCCTGCTGACAATTCTTCCATGAATTTTGGAGATCCGTTGTCGTTTCCAACTCGAGATCCATTTCTTCTTCCCCATTTTTCTCCCTTCAACTTTGCTTCGGATGGCGGCGGTGAGGGTTTGGCGGCGGACGACCAGCCGTCTAATTTACTTTCCCATATGTTGCAGATCTCTCCGAGTTCTGGTGATGGTGTAATTAGTACTCCTCCATGTGAGTCGCTCGCCACAGCAGTGGGGAATTCTCCGAGGTCGACTTCGGTTCGTGGTGGCGGTGGTCTTGTGCTTCCGACCGGTGGCTCGAACTCTCTTTGTTTAATGGAGAATTCCGGGATTCAGATCTCTTCTCCGCGAAATTCCGCCAATAAAAGAAGGAAGAGCCAAGTGAAGAAGGTGGTGTGTATTCCGGCACCGGCACCAGCGAACAGTCGATCAAGCAGCGGCGAAGTCGTTCCTTCTGATCTATGGGCATGGAGGAAGTACGGACAAAAGCCCATCAAAGGATCCCCATATCCAAGGGGATATTATAGGTGCAGTAGTTCCAAGGGCTGCTCCGCTCGAAAGCAGGTCGAGCGAAGTCGTACTAACCCCAACATGCTTGTCATCACCTACACTTCGGAGCACAACCACCCATGGCCCACTCAACGCAATGCCCTTGCAGGTTCTACTCGTTCCCATCCTTCACGAACCACCACCGCTACCACCGCTAATAAAACAAGCCCGAAACATGAGCGCATTAACGACATCCTGCCAACCACAACAATAAAGGAGGAAGAGATGGATGAAGACCAAATGACAGATAAAGCAACAACAACAAATGATAATCAGGAGGAGGGAGATCATCAAGATTTCCCTTATGACTTAATTTTCACTGAATTTGCAGATCAAATGAACGACCTCGAGCACGACAACAACCACGACCATAATCATCATCTTTCAGCAGACCCTCTGAACAACAATATGTTGATGTTTAGTAACCATGGATTTAGTGCAGGAAATGGAGAAGGCA

Coding sequence (CDS)

ATGGACAATAACAATTATCAGCAAGCTGGTGATTTGACTGATGTGATCCGACTGAGTTCCGCCCCTGTCGCCGGTCATTTCTCCTCCGAATTCTCTCTCGACCCGTTTTCCGGTGACAGACAACTCTGGTCGCACCTTCCTGCTGACAATTCTTCCATGAATTTTGGAGATCCGTTGTCGTTTCCAACTCGAGATCCATTTCTTCTTCCCCATTTTTCTCCCTTCAACTTTGCTTCGGATGGCGGCGGTGAGGGTTTGGCGGCGGACGACCAGCCGTCTAATTTACTTTCCCATATGTTGCAGATCTCTCCGAGTTCTGGTGATGGTGTAATTAGTACTCCTCCATGTGAGTCGCTCGCCACAGCAGTGGGGAATTCTCCGAGGTCGACTTCGGTTCGTGGTGGCGGTGGTCTTGTGCTTCCGACCGGTGGCTCGAACTCTCTTTGTTTAATGGAGAATTCCGGGATTCAGATCTCTTCTCCGCGAAATTCCGCCAATAAAAGAAGGAAGAGCCAAGTGAAGAAGGTGGTGTGTATTCCGGCACCGGCACCAGCGAACAGTCGATCAAGCAGCGGCGAAGTCGTTCCTTCTGATCTATGGGCATGGAGGAAGTACGGACAAAAGCCCATCAAAGGATCCCCATATCCAAGGGGATATTATAGGTGCAGTAGTTCCAAGGGCTGCTCCGCTCGAAAGCAGGTCGAGCGAAGTCGTACTAACCCCAACATGCTTGTCATCACCTACACTTCGGAGCACAACCACCCATGGCCCACTCAACGCAATGCCCTTGCAGGTTCTACTCGTTCCCATCCTTCACGAACCACCACCGCTACCACCGCTAATAAAACAAGCCCGAAACATGAGCGCATTAACGACATCCTGCCAACCACAACAATAAAGGAGGAAGAGATGGATGAAGACCAAATGACAGATAAAGCAACAACAACAAATGATAATCAGGAGGAGGGAGATCATCAAGATTTCCCTTATGACTTAATTTTCACTGAATTTGCAGATCAAATGAACGACCTCGAGCACGACAACAACCACGACCATAATCATCATCTTTCAGCAGACCCTCTGAACAACAATATGTTGATGTTTAGTAACCATGGATTTAGTGCAGGAAATGGAGAAGGCA

Protein sequence

MDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSMNFGDPLSFPTRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSSGDGVISTPPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKHERINDILPTTTIKEEEMDEDQMTDKATTTNDNQEEGDHQDFPYDLIFTEFADQMNDLEHDNNHDHNHHLSADPLNNNMLMFSNHGFSAGNGEGX
BLAST of Cucsa.167500 vs. Swiss-Prot
Match: WRK14_ARATH (Probable WRKY transcription factor 14 OS=Arabidopsis thaliana GN=WRKY14 PE=2 SV=2)

HSP 1 Score: 240.4 bits (612), Expect = 3.3e-62
Identity = 169/399 (42.36%), Postives = 213/399 (53.38%), Query Frame = 1

Query: 1   MDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLW----SHLPADNSSMN-- 60
           +D  N+Q  GDLTDV+R     + GH      L P +    +W    SH     S +N  
Sbjct: 8   LDMENFQ--GDLTDVVR----GIGGHV-----LSPETPPSNIWPLPLSHPTPSPSDLNIN 67

Query: 61  -FGDPLSFPTRDPFLLPHFSP------FNFASDGGGE----------GLAADDQPSNLLS 120
            FGDP  F + D  LL   +       F+   D               +  +D   +  S
Sbjct: 68  PFGDP--FVSMDDPLLQELNSITNSGYFSTVGDNNNNIHNNNGFLVPKVFEEDHIKSQCS 127

Query: 121 HMLQISPSSGDGVISTPPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLME----N 180
              +I  S  + +  + PC S A +      + +    G + + T    +  L++    +
Sbjct: 128 IFPRIRISHSNIIHDSSPCNSPAMSAHVVAAAAAASPRGIINVDTNSPRNCLLVDGTTFS 187

Query: 181 SGIQISSPRNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGS 240
           S IQISSPRN   KRRKSQ KKVVCIPAPA  NSRS SGEVVPSDLWAWRKYGQKPIKGS
Sbjct: 188 SQIQISSPRNLGLKRRKSQAKKVVCIPAPAAMNSRS-SGEVVPSDLWAWRKYGQKPIKGS 247

Query: 241 PYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSR 300
           P+PRGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWP QRNALAGSTR   S 
Sbjct: 248 PFPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRNALAGSTR---SS 307

Query: 301 TTTATTANKTSPKHERIND---------ILPTTTIK---------EEEMDEDQMTDKATT 349
           T++++  N + P    +N           LP++T           ++E  +D   +    
Sbjct: 308 TSSSSNPNPSKPSTANVNSSSIGSQNTIYLPSSTTPPPTLSSSAIKDERGDDMELENVDD 367

BLAST of Cucsa.167500 vs. Swiss-Prot
Match: WRK35_ARATH (Probable WRKY transcription factor 35 OS=Arabidopsis thaliana GN=WRKY35 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 8.2e-61
Identity = 176/420 (41.90%), Postives = 215/420 (51.19%), Query Frame = 1

Query: 4   NNYQQAGDLTDVIR--------LSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSMN- 63
           +N+Q  GDLTDV+R         S  P  G   S  S  P S    L    P+  +S + 
Sbjct: 2   DNFQ--GDLTDVVRGIGSGHVSPSPGPPEGPSPSSMSPPPTS---DLHVEFPSAATSASC 61

Query: 64  ----FGDPLSFPTRDPFLLPHFSPFNFASDGGGEGLAA-------DDQPSNLLSHMLQIS 123
               FGDP     +DP +    S  + A D       A       DD   +  S   +I 
Sbjct: 62  LANPFGDPF-VSMKDPLIHLPASYISGAGDNKSNKSFAIFPKIFEDDHIKSQCSVFPRIK 121

Query: 124 PSSGDGVISTPPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMEN------SGIQ 183
            S  + +     C S A  V ++  + S  G   +       N L +  N      S +Q
Sbjct: 122 ISQSNNIHDASTCNSPAITVSSAAVAASPWGMINVNTTNSPRNCLLVDNNNNTSSCSQVQ 181

Query: 184 ISS-PRNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYP 243
           ISS PRN   KRRKSQ KKVVCIPAPA  NSRS SGEVVPSDLWAWRKYGQKPIKGSPYP
Sbjct: 182 ISSSPRNLGIKRRKSQAKKVVCIPAPAAMNSRS-SGEVVPSDLWAWRKYGQKPIKGSPYP 241

Query: 244 RGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPS---- 303
           RGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTRS  S    
Sbjct: 242 RGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSSSSLN 301

Query: 304 -RTTTATTANKTSP----------KHERINDILPTTTIKEEEMDEDQMTDKATTTNDNQE 363
             + ++T A  TSP          K E  N  LP+++            D A    +N E
Sbjct: 302 PSSKSSTAAATTSPSSRVFQNNSSKDEPNNSNLPSSSTHPP-------FDAAAIKEENVE 361

Query: 364 E-GDHQDFPYDLIFTEFADQMNDLEHDNNHDHNHHLSADPLNNNML-MFSNHGFSAGNGE 380
           E  +  +F Y+ +   +  ++  L+   +   +     D L  + L M  +H    GN E
Sbjct: 362 ERQEKMEFDYNDVENTYRPEL--LQEFQHQPEDFFADLDELEGDSLTMLLSHSSGGGNME 405

BLAST of Cucsa.167500 vs. Swiss-Prot
Match: WRK22_ARATH (WRKY transcription factor 22 OS=Arabidopsis thaliana GN=WRKY22 PE=2 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.7e-37
Identity = 89/195 (45.64%), Postives = 113/195 (57.95%), Query Frame = 1

Query: 141 PTGGSNSLCLMENSGIQISSP---RNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPS 200
           P      L L  +SG   S P     S +KRRK Q KKV  + A           E + S
Sbjct: 80  PQNQKRPLSLSASSGSVTSKPSGSNTSRSKRRKIQHKKVCHVAA-----------EALNS 139

Query: 201 DLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWP 260
           D+WAWRKYGQKPIKGSPYPRGYYRCS+SKGC ARKQVER+R++P M ++TYT+EHNHP P
Sbjct: 140 DVWAWRKYGQKPIKGSPYPRGYYRCSTSKGCLARKQVERNRSDPKMFIVTYTAEHNHPAP 199

Query: 261 TQRNALAGSTRSHPS------RTTTATTANKTSPKHERINDILPT---TTIKEEEMDEDQ 320
           T RN+LAGSTR  PS        TT      +SP       +LP      + + + +ED 
Sbjct: 200 THRNSLAGSTRQKPSDQQTSKSPTTTIATYSSSPVTSADEFVLPVEDHLAVGDLDGEEDL 259

Query: 321 MTDKATTTNDNQEEG 324
           ++   T  +D+  +G
Sbjct: 260 LSLSDTVVSDDFFDG 263

BLAST of Cucsa.167500 vs. Swiss-Prot
Match: WRK27_ARATH (Probable WRKY transcription factor 27 OS=Arabidopsis thaliana GN=WRKY27 PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.6e-35
Identity = 84/201 (41.79%), Postives = 115/201 (57.21%), Query Frame = 1

Query: 139 VLPTGGSNSLCLMENSGIQISSPRNSA---NKRRKSQVKKVVCIPAPAPANSRSSSGEVV 198
           V P   S+S+ +      Q+   ++     +++RK+Q K+ +C            + E +
Sbjct: 114 VFPPSTSSSVFVFRGQRDQLLQQQSQPPLRSRKRKNQQKRTIC----------HVTQENL 173

Query: 199 PSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHP 258
            SDLWAWRKYGQKPIKGSPYPR YYRCSSSKGC ARKQVERS  +PN+ ++TYT EH HP
Sbjct: 174 SSDLWAWRKYGQKPIKGSPYPRNYYRCSSSKGCLARKQVERSNLDPNIFIVTYTGEHTHP 233

Query: 259 WPTQRNALAGSTRSHPSRTTTATTANKTSPKHERIND---ILPTTTIK------EEEMDE 318
            PT RN+LAGSTR + S+         TSP  + + +   + PTT +K      E   DE
Sbjct: 234 RPTHRNSLAGSTR-NKSQPVNPVPKPDTSPLSDTVKEEIHLSPTTPLKGNDDVQETNGDE 293

Query: 319 DQMTDKATTTNDNQEEGDHQD 328
           D +  +     + +EE   +D
Sbjct: 294 DMVGQEVNMEEEEEEEEVEED 303

BLAST of Cucsa.167500 vs. Swiss-Prot
Match: WRK16_ARATH (Probable WRKY transcription factor 16 OS=Arabidopsis thaliana GN=WRKY16 PE=2 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 4.5e-35
Identity = 80/155 (51.61%), Postives = 102/155 (65.81%), Query Frame = 1

Query: 164  SANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCS 223
            S ++R+ ++ K+VVC+       SRSS       DLW WRKYGQKPIK SPYPR YYRC+
Sbjct: 1157 SKSRRKNNKEKRVVCV---VDRGSRSS-------DLWVWRKYGQKPIKSSPYPRSYYRCA 1216

Query: 224  SSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSR---TTTATTA 283
            SSKGC ARKQVERSRT+PN+ VITY SEHNHP+PT RN LAGSTRS  S+    TT+ ++
Sbjct: 1217 SSKGCFARKQVERSRTDPNVSVITYISEHNHPFPTLRNTLAGSTRSSSSKCSDVTTSASS 1276

Query: 284  NKTSPKHERINDILPTT---------TIKEEEMDE 307
              +  K       LP++          +KEE+M++
Sbjct: 1277 TVSQDKEGPDKSHLPSSPASPPYAAMVVKEEDMEQ 1301

BLAST of Cucsa.167500 vs. TrEMBL
Match: A0A0A0LYP6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G547510 PE=4 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 9.6e-226
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 1

Query: 1   MDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSMNFGDPLS 60
           MDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSMNFGDPLS
Sbjct: 8   MDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSMNFGDPLS 67

Query: 61  FPTRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSSGDGVISTPPCESLA 120
           FPTRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSSGDGVISTPPCESLA
Sbjct: 68  FPTRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSSGDGVISTPPCESLA 127

Query: 121 TAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNSANKRRKSQVKKVVCIP 180
           TAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNSANKRRKSQVKKVVCIP
Sbjct: 128 TAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNSANKRRKSQVKKVVCIP 187

Query: 181 APAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTN 240
           APAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTN
Sbjct: 188 APAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTN 247

Query: 241 PNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKHERINDILPTTTIK 300
           PNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKHERINDILPTTTIK
Sbjct: 248 PNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKHERINDILPTTTIK 307

Query: 301 EEEMDEDQMTDKATTTNDNQEEGDHQDFPYDLIFTEFADQMNDLEHDNNHDHNHHLSADP 360
           EEEMDEDQMTDKATTTNDNQEEGDHQDFPYDLIFTEFADQMNDLEHDNNHDHNHHLSADP
Sbjct: 308 EEEMDEDQMTDKATTTNDNQEEGDHQDFPYDLIFTEFADQMNDLEHDNNHDHNHHLSADP 367

Query: 361 LNNNMLMFSNHGFSAGNGEG 381
           LNNNMLMFSNHGFSAGNGEG
Sbjct: 368 LNNNMLMFSNHGFSAGNGEG 387

BLAST of Cucsa.167500 vs. TrEMBL
Match: M5WIC8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004535mg PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 2.0e-69
Identity = 177/383 (46.21%), Postives = 217/383 (56.66%), Query Frame = 1

Query: 14  DVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSMNFGDP--LSFPTRDPFLLPH 73
           D+   SS  ++ + SS+ +++  S D        A  +S  FG    +  P   P   PH
Sbjct: 93  DISSSSSFFISPNSSSDMNINITSVDE-------AGGTSSTFGGAHIVLAPPPPP---PH 152

Query: 74  FSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSSGDGVISTP-PCESLATAVGNSPRST 133
               +    GG        +P N+ S MLQISPS+   V +   P    ATAV +SPR  
Sbjct: 153 HHHLHHHQQGGVVDGDPIKRPCNIFSRMLQISPSAKVPVTACDSPLLGAATAV-SSPRGI 212

Query: 134 SVRGGGGLVLPTGGSNSL----------CLMENSGIQISSPRNSANKRRKSQVKKVVCIP 193
           S    G +     GS+ +          CL+EN+G+QISSPRN   KRRKSQ KKVVCIP
Sbjct: 213 SKAVSGSISGAMVGSDMIINGNSSSGKGCLLENTGVQISSPRNPGIKRRKSQAKKVVCIP 272

Query: 194 APAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTN 253
           APA ANSR  +GEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRT+
Sbjct: 273 APAAANSRP-TGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTD 332

Query: 254 PNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKHERINDILPTTTIK 313
           PNMLVITYTSEHNHPWPTQRNALAGSTRS PS+++  +  +  S          PT   +
Sbjct: 333 PNMLVITYTSEHNHPWPTQRNALAGSTRSQPSKSSAGSKTSSNSSHQPHQKPASPTKEEQ 392

Query: 314 EEEMDEDQMTDKATTTN------------DNQEEGDHQ-DFPYDLIFTEFADQMNDLEHD 370
            E M+++       T              + Q E DHQ  FPY     +  DQ N   H+
Sbjct: 393 NETMNDNNHVSPVITAGSASTVKEEFEDIEKQLELDHQAGFPYRPSMPD--DQSNQSHHN 452

BLAST of Cucsa.167500 vs. TrEMBL
Match: A0A0D2UKC0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_011G006600 PE=4 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.3e-68
Identity = 185/379 (48.81%), Postives = 211/379 (55.67%), Query Frame = 1

Query: 1   MDNNNYQQAGDLTDVIRLSSAPVAGHFSS----------EF-SLDPFSGDRQLWSHLPAD 60
           ++  NYQ  GDL D++R S+ P   HFS           EF S DP S      S +  D
Sbjct: 5   LEMENYQ--GDLNDIVRASAEPA--HFSDMVPQLSSWQQEFPSSDPLSFSS---SAMEDD 64

Query: 61  NSSMNFGDPLSFPTRDPFLLPHFSPFNFASDGGGE-----------------GLAADDQP 120
            S+  FGDP S   RDP LL      N    G                    G      P
Sbjct: 65  YSTNTFGDPFS-AIRDPLLL--LQELNVVGTGSSSTYFNNSPNSTNDHHHMGGFDDMTSP 124

Query: 121 SNLLSHMLQISPSSGDGVISTPPCESLATAVGNSPRSTSVRGGGG----LVLPTGGSNSL 180
            N+ S  +QIS SS       PPC+    A  ++       G GG     V P+   N+ 
Sbjct: 125 CNIFSR-IQISHSSNP----LPPCDPPRMAAAST-------GLGGHKAPAVFPSDMVNAK 184

Query: 181 -CLMENSG------IQISSPRNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWA 240
            CL++N+       +QISSPRN   KRRKSQ KKVVCIPAPA ANSRS SGEVVPSDLWA
Sbjct: 185 SCLIDNTAAAASVPVQISSPRNLGIKRRKSQGKKVVCIPAPAAANSRS-SGEVVPSDLWA 244

Query: 241 WRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRN 300
           WRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWPTQRN
Sbjct: 245 WRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRN 304

Query: 301 ALAGSTRSHPSRTTTATTANKTSPKHERINDILPTTTIKEEEMDEDQMTDKA-------- 333
           ALAGSTRS PS+      AN T  K    +D  P  T KE   +E +M D A        
Sbjct: 305 ALAGSTRSQPSK----PNANNTGSKMGSPSDSEPQKTTKETIKEETEMDDMAKQQLDIDE 356

BLAST of Cucsa.167500 vs. TrEMBL
Match: A0A0D5YA14_SALMI (WRKY protein OS=Salvia miltiorrhiza GN=WRKY40 PE=2 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 8.2e-68
Identity = 173/376 (46.01%), Postives = 218/376 (57.98%), Query Frame = 1

Query: 5   NYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSMNFGDPLSFPTR 64
           NYQ  GDLTD+IR +        + ++    F  +   +S  PAD+    FGDP +   R
Sbjct: 3   NYQ--GDLTDIIRATGGAETPAAAPDWH---FPANPITFSTNPADD----FGDPFAH-LR 62

Query: 65  DPFL-------LPHF-------SPFNFASDGGGEGLAADD--QPSNLLSHMLQISPSSGD 124
           DP +       LP F        P +      G  +  D+  +P N+ S MLQISP+   
Sbjct: 63  DPLIHIDAPPPLPGFFHGSDVIKPSSVDDSNLGHKIIDDEMKRPPNIFSRMLQISPTPKP 122

Query: 125 GVISTPPCESLATAVGNSPRSTSVRGGGGLVLPT-----GGSNSL-CLMENSGIQISSPR 184
            +    PC+S           T ++G   +++P        SNS  CLME+S +QISSPR
Sbjct: 123 PLQLPLPCDSPVVVA-----PTGLKGVAPMLVPNDHTLISPSNSRGCLMESS-LQISSPR 182

Query: 185 NSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRC 244
           N+  +RRKSQ KKVVCIPAPAPANSR  SGE+VPSDLWAWRKYGQKPIKGSPYPRGYYRC
Sbjct: 183 NTGIRRRKSQAKKVVCIPAPAPANSRP-SGEIVPSDLWAWRKYGQKPIKGSPYPRGYYRC 242

Query: 245 SSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANK 304
           SSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTRS PS+         
Sbjct: 243 SSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSQPSK-------KS 302

Query: 305 TSPKHERINDILPTTTIKEEEMDEDQMTDKATTTNDNQEEGDHQDFPYDLIFTEFADQMN 353
            +PK E+       + +KEE  + +Q    A   + +    + +    D++FT+  D  +
Sbjct: 303 INPKEEQ-----KESGVKEEVDNNEQSYKPALPESQDSFFAEIEADSLDMLFTQGFDDRD 349

BLAST of Cucsa.167500 vs. TrEMBL
Match: A0A022RT22_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a017713mg PE=4 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 1.4e-67
Identity = 181/407 (44.47%), Postives = 224/407 (55.04%), Query Frame = 1

Query: 5   NYQQAGDLTDVIRLSS-----------APVAGHFSSEFSLDPFSGDRQLWSHLPADNSSM 64
           NYQ  GDL D+ R S+           A  A     +F  +P +     +S  P ++   
Sbjct: 3   NYQ--GDLADIFRPSTTTATVDGGDPDAAAAALPDWQFPTNPIT-----YSSDPVED--- 62

Query: 65  NFGDPLSFPTRDPFL--LPHFSPFNFAS--DGGG-------------EGLAADDQ---PS 124
            FGDP ++   +P L  +P F   N ++  DGGG               L   D+   PS
Sbjct: 63  -FGDPFNY-MGNPLLPAVPGFFSCNNSNIIDGGGGINNSGAGGVNISHRLLVHDEMRKPS 122

Query: 125 NLLSHMLQISPSSGDGVISTPPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMEN 184
           N+ S MLQISP++      +P   S A A   +  +  +R    LV      +  CL+E+
Sbjct: 123 NIFSRMLQISPNA-----KSPVDHSQAAAAAAASPTAGLRVSPILV------SKGCLVES 182

Query: 185 -SGIQISSPRNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKG 244
             G+QISSPRN+  KRRKSQ KKVVCIPA +PANSR+ +GE+VPSDLWAWRKYGQKPIKG
Sbjct: 183 PGGLQISSPRNTGIKRRKSQAKKVVCIPASSPANSRA-TGEIVPSDLWAWRKYGQKPIKG 242

Query: 245 SPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPS 304
           SPYPRGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTRS PS
Sbjct: 243 SPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSQPS 302

Query: 305 RTTTATTANKTSPKHERINDILPTTTIKEEEMDEDQMTDKATTTNDNQEEGDHQDFPYDL 364
             TT TT   T+ K        PT  I     +   +  +  TT+D  +E + +      
Sbjct: 303 SKTTTTTTTTTTKKD-------PTKQINNNNNNNTVIITQQFTTDDVLKEEEEE------ 362

Query: 365 IFTEFADQMNDLEHDNNHDHNHHLSADPLNNNMLMFSNHGFSAGNGE 380
              E      D +H    DH   L     N  +L   +H F A  GE
Sbjct: 363 ---EEITNNQDFDHQLEMDHKFELGFQ-YNPTLLEDDHHNFFADLGE 368

BLAST of Cucsa.167500 vs. TAIR10
Match: AT1G30650.1 (AT1G30650.1 WRKY DNA-binding protein 14)

HSP 1 Score: 240.4 bits (612), Expect = 1.9e-63
Identity = 169/399 (42.36%), Postives = 213/399 (53.38%), Query Frame = 1

Query: 1   MDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLW----SHLPADNSSMN-- 60
           +D  N+Q  GDLTDV+R     + GH      L P +    +W    SH     S +N  
Sbjct: 8   LDMENFQ--GDLTDVVR----GIGGHV-----LSPETPPSNIWPLPLSHPTPSPSDLNIN 67

Query: 61  -FGDPLSFPTRDPFLLPHFSP------FNFASDGGGE----------GLAADDQPSNLLS 120
            FGDP  F + D  LL   +       F+   D               +  +D   +  S
Sbjct: 68  PFGDP--FVSMDDPLLQELNSITNSGYFSTVGDNNNNIHNNNGFLVPKVFEEDHIKSQCS 127

Query: 121 HMLQISPSSGDGVISTPPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLME----N 180
              +I  S  + +  + PC S A +      + +    G + + T    +  L++    +
Sbjct: 128 IFPRIRISHSNIIHDSSPCNSPAMSAHVVAAAAAASPRGIINVDTNSPRNCLLVDGTTFS 187

Query: 181 SGIQISSPRNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGS 240
           S IQISSPRN   KRRKSQ KKVVCIPAPA  NSRS SGEVVPSDLWAWRKYGQKPIKGS
Sbjct: 188 SQIQISSPRNLGLKRRKSQAKKVVCIPAPAAMNSRS-SGEVVPSDLWAWRKYGQKPIKGS 247

Query: 241 PYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSR 300
           P+PRGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWP QRNALAGSTR   S 
Sbjct: 248 PFPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRNALAGSTR---SS 307

Query: 301 TTTATTANKTSPKHERIND---------ILPTTTIK---------EEEMDEDQMTDKATT 349
           T++++  N + P    +N           LP++T           ++E  +D   +    
Sbjct: 308 TSSSSNPNPSKPSTANVNSSSIGSQNTIYLPSSTTPPPTLSSSAIKDERGDDMELENVDD 367

BLAST of Cucsa.167500 vs. TAIR10
Match: AT2G34830.1 (AT2G34830.1 WRKY DNA-binding protein 35)

HSP 1 Score: 235.7 bits (600), Expect = 4.6e-62
Identity = 176/420 (41.90%), Postives = 215/420 (51.19%), Query Frame = 1

Query: 4   NNYQQAGDLTDVIR--------LSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSMN- 63
           +N+Q  GDLTDV+R         S  P  G   S  S  P S    L    P+  +S + 
Sbjct: 2   DNFQ--GDLTDVVRGIGSGHVSPSPGPPEGPSPSSMSPPPTS---DLHVEFPSAATSASC 61

Query: 64  ----FGDPLSFPTRDPFLLPHFSPFNFASDGGGEGLAA-------DDQPSNLLSHMLQIS 123
               FGDP     +DP +    S  + A D       A       DD   +  S   +I 
Sbjct: 62  LANPFGDPF-VSMKDPLIHLPASYISGAGDNKSNKSFAIFPKIFEDDHIKSQCSVFPRIK 121

Query: 124 PSSGDGVISTPPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMEN------SGIQ 183
            S  + +     C S A  V ++  + S  G   +       N L +  N      S +Q
Sbjct: 122 ISQSNNIHDASTCNSPAITVSSAAVAASPWGMINVNTTNSPRNCLLVDNNNNTSSCSQVQ 181

Query: 184 ISS-PRNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYP 243
           ISS PRN   KRRKSQ KKVVCIPAPA  NSRS SGEVVPSDLWAWRKYGQKPIKGSPYP
Sbjct: 182 ISSSPRNLGIKRRKSQAKKVVCIPAPAAMNSRS-SGEVVPSDLWAWRKYGQKPIKGSPYP 241

Query: 244 RGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPS---- 303
           RGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTRS  S    
Sbjct: 242 RGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSSSSLN 301

Query: 304 -RTTTATTANKTSP----------KHERINDILPTTTIKEEEMDEDQMTDKATTTNDNQE 363
             + ++T A  TSP          K E  N  LP+++            D A    +N E
Sbjct: 302 PSSKSSTAAATTSPSSRVFQNNSSKDEPNNSNLPSSSTHPP-------FDAAAIKEENVE 361

Query: 364 E-GDHQDFPYDLIFTEFADQMNDLEHDNNHDHNHHLSADPLNNNML-MFSNHGFSAGNGE 380
           E  +  +F Y+ +   +  ++  L+   +   +     D L  + L M  +H    GN E
Sbjct: 362 ERQEKMEFDYNDVENTYRPEL--LQEFQHQPEDFFADLDELEGDSLTMLLSHSSGGGNME 405

BLAST of Cucsa.167500 vs. TAIR10
Match: AT4G01250.1 (AT4G01250.1 WRKY family transcription factor)

HSP 1 Score: 157.1 bits (396), Expect = 2.1e-38
Identity = 89/195 (45.64%), Postives = 113/195 (57.95%), Query Frame = 1

Query: 141 PTGGSNSLCLMENSGIQISSP---RNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPS 200
           P      L L  +SG   S P     S +KRRK Q KKV  + A           E + S
Sbjct: 80  PQNQKRPLSLSASSGSVTSKPSGSNTSRSKRRKIQHKKVCHVAA-----------EALNS 139

Query: 201 DLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWP 260
           D+WAWRKYGQKPIKGSPYPRGYYRCS+SKGC ARKQVER+R++P M ++TYT+EHNHP P
Sbjct: 140 DVWAWRKYGQKPIKGSPYPRGYYRCSTSKGCLARKQVERNRSDPKMFIVTYTAEHNHPAP 199

Query: 261 TQRNALAGSTRSHPS------RTTTATTANKTSPKHERINDILPT---TTIKEEEMDEDQ 320
           T RN+LAGSTR  PS        TT      +SP       +LP      + + + +ED 
Sbjct: 200 THRNSLAGSTRQKPSDQQTSKSPTTTIATYSSSPVTSADEFVLPVEDHLAVGDLDGEEDL 259

Query: 321 MTDKATTTNDNQEEG 324
           ++   T  +D+  +G
Sbjct: 260 LSLSDTVVSDDFFDG 263

BLAST of Cucsa.167500 vs. TAIR10
Match: AT5G52830.1 (AT5G52830.1 WRKY DNA-binding protein 27)

HSP 1 Score: 151.8 bits (382), Expect = 8.8e-37
Identity = 84/201 (41.79%), Postives = 115/201 (57.21%), Query Frame = 1

Query: 139 VLPTGGSNSLCLMENSGIQISSPRNSA---NKRRKSQVKKVVCIPAPAPANSRSSSGEVV 198
           V P   S+S+ +      Q+   ++     +++RK+Q K+ +C            + E +
Sbjct: 114 VFPPSTSSSVFVFRGQRDQLLQQQSQPPLRSRKRKNQQKRTIC----------HVTQENL 173

Query: 199 PSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHP 258
            SDLWAWRKYGQKPIKGSPYPR YYRCSSSKGC ARKQVERS  +PN+ ++TYT EH HP
Sbjct: 174 SSDLWAWRKYGQKPIKGSPYPRNYYRCSSSKGCLARKQVERSNLDPNIFIVTYTGEHTHP 233

Query: 259 WPTQRNALAGSTRSHPSRTTTATTANKTSPKHERIND---ILPTTTIK------EEEMDE 318
            PT RN+LAGSTR + S+         TSP  + + +   + PTT +K      E   DE
Sbjct: 234 RPTHRNSLAGSTR-NKSQPVNPVPKPDTSPLSDTVKEEIHLSPTTPLKGNDDVQETNGDE 293

Query: 319 DQMTDKATTTNDNQEEGDHQD 328
           D +  +     + +EE   +D
Sbjct: 294 DMVGQEVNMEEEEEEEEVEED 303

BLAST of Cucsa.167500 vs. TAIR10
Match: AT5G45050.1 (AT5G45050.1 Disease resistance protein (TIR-NBS-LRR class))

HSP 1 Score: 150.2 bits (378), Expect = 2.6e-36
Identity = 80/155 (51.61%), Postives = 102/155 (65.81%), Query Frame = 1

Query: 164  SANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCS 223
            S ++R+ ++ K+VVC+       SRSS       DLW WRKYGQKPIK SPYPR YYRC+
Sbjct: 1157 SKSRRKNNKEKRVVCV---VDRGSRSS-------DLWVWRKYGQKPIKSSPYPRSYYRCA 1216

Query: 224  SSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSR---TTTATTA 283
            SSKGC ARKQVERSRT+PN+ VITY SEHNHP+PT RN LAGSTRS  S+    TT+ ++
Sbjct: 1217 SSKGCFARKQVERSRTDPNVSVITYISEHNHPFPTLRNTLAGSTRSSSSKCSDVTTSASS 1276

Query: 284  NKTSPKHERINDILPTT---------TIKEEEMDE 307
              +  K       LP++          +KEE+M++
Sbjct: 1277 TVSQDKEGPDKSHLPSSPASPPYAAMVVKEEDMEQ 1301

BLAST of Cucsa.167500 vs. NCBI nr
Match: gi|778662047|ref|XP_011659293.1| (PREDICTED: probable WRKY transcription factor 14 [Cucumis sativus])

HSP 1 Score: 790.4 bits (2040), Expect = 1.4e-225
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 1

Query: 1   MDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSMNFGDPLS 60
           MDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSMNFGDPLS
Sbjct: 8   MDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSMNFGDPLS 67

Query: 61  FPTRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSSGDGVISTPPCESLA 120
           FPTRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSSGDGVISTPPCESLA
Sbjct: 68  FPTRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSSGDGVISTPPCESLA 127

Query: 121 TAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNSANKRRKSQVKKVVCIP 180
           TAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNSANKRRKSQVKKVVCIP
Sbjct: 128 TAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNSANKRRKSQVKKVVCIP 187

Query: 181 APAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTN 240
           APAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTN
Sbjct: 188 APAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTN 247

Query: 241 PNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKHERINDILPTTTIK 300
           PNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKHERINDILPTTTIK
Sbjct: 248 PNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKHERINDILPTTTIK 307

Query: 301 EEEMDEDQMTDKATTTNDNQEEGDHQDFPYDLIFTEFADQMNDLEHDNNHDHNHHLSADP 360
           EEEMDEDQMTDKATTTNDNQEEGDHQDFPYDLIFTEFADQMNDLEHDNNHDHNHHLSADP
Sbjct: 308 EEEMDEDQMTDKATTTNDNQEEGDHQDFPYDLIFTEFADQMNDLEHDNNHDHNHHLSADP 367

Query: 361 LNNNMLMFSNHGFSAGNGEG 381
           LNNNMLMFSNHGFSAGNGEG
Sbjct: 368 LNNNMLMFSNHGFSAGNGEG 387

BLAST of Cucsa.167500 vs. NCBI nr
Match: gi|659131380|ref|XP_008465657.1| (PREDICTED: probable WRKY transcription factor 14 isoform X1 [Cucumis melo])

HSP 1 Score: 728.8 bits (1880), Expect = 4.9e-207
Identity = 353/381 (92.65%), Postives = 365/381 (95.80%), Query Frame = 1

Query: 1   MDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSMNFGDPLS 60
           MDNNNYQQAGDLTDVIR +SAPVAGHFSSEFSLD FSG+ +LWSHLPADNSSMNFGDPLS
Sbjct: 8   MDNNNYQQAGDLTDVIRPTSAPVAGHFSSEFSLDSFSGEGRLWSHLPADNSSMNFGDPLS 67

Query: 61  FPTRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSSGDGVISTPPCESLA 120
           F TRDPFLLPHFSPFNFASDGGGEGLAAD QPSNLLSHMLQISPSSGDGVISTPPCESLA
Sbjct: 68  FQTRDPFLLPHFSPFNFASDGGGEGLAAD-QPSNLLSHMLQISPSSGDGVISTPPCESLA 127

Query: 121 TAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNSANKRRKSQVKKVVCIP 180
           +AVGNSP STSVRGGGG+V+PTGGSN LCLMENSGIQISSPRNSANKRRKSQVKKVVCIP
Sbjct: 128 SAVGNSPSSTSVRGGGGVVVPTGGSNPLCLMENSGIQISSPRNSANKRRKSQVKKVVCIP 187

Query: 181 APAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTN 240
           APAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTN
Sbjct: 188 APAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTN 247

Query: 241 PNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKHERINDILPTTTIK 300
           PNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTT T  NKTSPK ERINDILPTT IK
Sbjct: 248 PNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTTTATNKTSPKQERINDILPTTMIK 307

Query: 301 EEEMDEDQMTDK-ATTTNDNQEEGDHQDFPYDLIFTEFADQMNDLEHDNNHDHNHHLSAD 360
           EEE+DEDQ TDK ATTTNDNQEEGDHQDFP+DLIFTEFADQMNDLEH+N+HDH+HHLSAD
Sbjct: 308 EEEIDEDQTTDKAATTTNDNQEEGDHQDFPFDLIFTEFADQMNDLEHENDHDHSHHLSAD 367

Query: 361 PLNNNMLMFSNHGFSAGNGEG 381
           PLNNNMLMF +HGFS GNGEG
Sbjct: 368 PLNNNMLMFGSHGFSTGNGEG 387

BLAST of Cucsa.167500 vs. NCBI nr
Match: gi|659131382|ref|XP_008465658.1| (PREDICTED: probable WRKY transcription factor 14 isoform X2 [Cucumis melo])

HSP 1 Score: 713.4 bits (1840), Expect = 2.1e-202
Identity = 349/381 (91.60%), Postives = 361/381 (94.75%), Query Frame = 1

Query: 1   MDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSMNFGDPLS 60
           MDNNNYQQAGDLTDVIR +SAPVAGHFSSEFSLD FSG+ +LWSHLPADNSSMNFGDPLS
Sbjct: 8   MDNNNYQQAGDLTDVIRPTSAPVAGHFSSEFSLDSFSGEGRLWSHLPADNSSMNFGDPLS 67

Query: 61  FPTRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSSGDGVISTPPCESLA 120
           F TRDPFLLPHFSPFNFASDGGGEGLAAD QPSNLLSHMLQISPSSGDGVISTPPCESLA
Sbjct: 68  FQTRDPFLLPHFSPFNFASDGGGEGLAAD-QPSNLLSHMLQISPSSGDGVISTPPCESLA 127

Query: 121 TAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNSANKRRKSQVKKVVCIP 180
           +AVGNSP STSVRGGGG+V+PTGGSN LCLMENSGIQISSPRNSANKRRKSQVKKVVCIP
Sbjct: 128 SAVGNSPSSTSVRGGGGVVVPTGGSNPLCLMENSGIQISSPRNSANKRRKSQVKKVVCIP 187

Query: 181 APAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTN 240
           APAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYP    RCSSSKGCSARKQVERSRTN
Sbjct: 188 APAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYP----RCSSSKGCSARKQVERSRTN 247

Query: 241 PNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKHERINDILPTTTIK 300
           PNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTT T  NKTSPK ERINDILPTT IK
Sbjct: 248 PNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTTTATNKTSPKQERINDILPTTMIK 307

Query: 301 EEEMDEDQMTDK-ATTTNDNQEEGDHQDFPYDLIFTEFADQMNDLEHDNNHDHNHHLSAD 360
           EEE+DEDQ TDK ATTTNDNQEEGDHQDFP+DLIFTEFADQMNDLEH+N+HDH+HHLSAD
Sbjct: 308 EEEIDEDQTTDKAATTTNDNQEEGDHQDFPFDLIFTEFADQMNDLEHENDHDHSHHLSAD 367

Query: 361 PLNNNMLMFSNHGFSAGNGEG 381
           PLNNNMLMF +HGFS GNGEG
Sbjct: 368 PLNNNMLMFGSHGFSTGNGEG 383

BLAST of Cucsa.167500 vs. NCBI nr
Match: gi|698484442|ref|XP_009789029.1| (PREDICTED: probable WRKY transcription factor 14 [Nicotiana sylvestris])

HSP 1 Score: 290.4 bits (742), Expect = 4.5e-75
Identity = 156/285 (54.74%), Postives = 192/285 (67.37%), Query Frame = 1

Query: 94  NLLSHMLQISPSSGDGVISTPPCESLATAVGNSPRSTSVRGGGGLV----LPTGGSNSLC 153
           N+ S MLQISP++      +P    +  AV   P ST + GGG LV    + +  S+  C
Sbjct: 142 NIFSRMLQISPTTKLAAAMSPCNSPMTGAVALQPPSTVIAGGGALVANDAIISPNSSKTC 201

Query: 154 LMENSGIQISSPRNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKP 213
           LMENSG+QISSPRN+  KRRKSQ KKVVCIPAPAPANSR   GE+VPSDLWAWRKYGQKP
Sbjct: 202 LMENSGLQISSPRNTGIKRRKSQAKKVVCIPAPAPANSRQG-GEIVPSDLWAWRKYGQKP 261

Query: 214 IKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRS 273
           IKGSPYPRGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWPTQRN+LAGSTRS
Sbjct: 262 IKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNSLAGSTRS 321

Query: 274 HPS---RTTTATTANKTSPKHERINDILPTTTIKEEEMDEDQMTDKATTTNDNQEEGDHQ 333
            P+    TTT+T+ N T+ K ++ N+I     +   ++ E+          D +++  HQ
Sbjct: 322 QPNNSKHTTTSTSKNNTNSKDDQQNNISTVAQVNISKVKEE-----VAELQDLRDDHHHQ 381

Query: 334 DFPYDLIFTEFA-DQMNDLEHDNNHDHNH--------HLSADPLN 363
               ++   EF+ D    +  D++ +H+H         L ADPLN
Sbjct: 382 QLAVEMRDVEFSKDSYQPILPDSSSNHSHEDFFADLVELEADPLN 420

BLAST of Cucsa.167500 vs. NCBI nr
Match: gi|697136922|ref|XP_009622546.1| (PREDICTED: probable WRKY transcription factor 14 [Nicotiana tomentosiformis])

HSP 1 Score: 281.6 bits (719), Expect = 2.1e-72
Identity = 157/286 (54.90%), Postives = 193/286 (67.48%), Query Frame = 1

Query: 94  NLLSHMLQISPSSGDGVISTPPCES-LATAVGNSPRSTSVRGGGGLVLPTG----GSNSL 153
           N+ S MLQISP++    +S  PC S +  AV   P ST +  G  LV         S+  
Sbjct: 141 NIFSRMLQISPTTKLAAMS--PCNSPMTAAVALQPPSTVIATGDALVANDAIMFPNSSKT 200

Query: 154 CLMENSGIQISSPRNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQK 213
           CL+ENSG+QISSPRN+  KRRKSQ KKVVCIPAPAPANSR   GE+VPSDLWAWRKYGQK
Sbjct: 201 CLVENSGLQISSPRNTGIKRRKSQAKKVVCIPAPAPANSRQG-GEIVPSDLWAWRKYGQK 260

Query: 214 PIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTR 273
           PIKGSPYPRGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTR
Sbjct: 261 PIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTR 320

Query: 274 SHPS---RTTTATTANKTSPKHERINDILPTTTIKEEEMDEDQMTDKATTTNDNQEEGDH 333
           S P+    TTT+T+ N T+ K +  N    T+T+   +++  ++ ++     D + +  H
Sbjct: 321 SQPNNSKHTTTSTSKNNTNSKDDEQN----TSTV--AQVNISKVKEEVAELQDLRHDHHH 380

Query: 334 QDFPYDLIFTEFA-DQMNDLEHDNNHDHNH--------HLSADPLN 363
           Q    ++   EF+ D    +  D++ +H+H         L ADPLN
Sbjct: 381 QQLAVEMRDFEFSKDSYQPILPDSSSNHSHEDFFADLVELEADPLN 417

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
WRK14_ARATH3.3e-6242.36Probable WRKY transcription factor 14 OS=Arabidopsis thaliana GN=WRKY14 PE=2 SV=... [more]
WRK35_ARATH8.2e-6141.90Probable WRKY transcription factor 35 OS=Arabidopsis thaliana GN=WRKY35 PE=2 SV=... [more]
WRK22_ARATH3.7e-3745.64WRKY transcription factor 22 OS=Arabidopsis thaliana GN=WRKY22 PE=2 SV=1[more]
WRK27_ARATH1.6e-3541.79Probable WRKY transcription factor 27 OS=Arabidopsis thaliana GN=WRKY27 PE=2 SV=... [more]
WRK16_ARATH4.5e-3551.61Probable WRKY transcription factor 16 OS=Arabidopsis thaliana GN=WRKY16 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0LYP6_CUCSA9.6e-226100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G547510 PE=4 SV=1[more]
M5WIC8_PRUPE2.0e-6946.21Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004535mg PE=4 SV=1[more]
A0A0D2UKC0_GOSRA1.3e-6848.81Uncharacterized protein OS=Gossypium raimondii GN=B456_011G006600 PE=4 SV=1[more]
A0A0D5YA14_SALMI8.2e-6846.01WRKY protein OS=Salvia miltiorrhiza GN=WRKY40 PE=2 SV=1[more]
A0A022RT22_ERYGU1.4e-6744.47Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a017713mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G30650.11.9e-6342.36 WRKY DNA-binding protein 14[more]
AT2G34830.14.6e-6241.90 WRKY DNA-binding protein 35[more]
AT4G01250.12.1e-3845.64 WRKY family transcription factor[more]
AT5G52830.18.8e-3741.79 WRKY DNA-binding protein 27[more]
AT5G45050.12.6e-3651.61 Disease resistance protein (TIR-NBS-LRR class)[more]
Match NameE-valueIdentityDescription
gi|778662047|ref|XP_011659293.1|1.4e-225100.00PREDICTED: probable WRKY transcription factor 14 [Cucumis sativus][more]
gi|659131380|ref|XP_008465657.1|4.9e-20792.65PREDICTED: probable WRKY transcription factor 14 isoform X1 [Cucumis melo][more]
gi|659131382|ref|XP_008465658.1|2.1e-20291.60PREDICTED: probable WRKY transcription factor 14 isoform X2 [Cucumis melo][more]
gi|698484442|ref|XP_009789029.1|4.5e-7554.74PREDICTED: probable WRKY transcription factor 14 [Nicotiana sylvestris][more]
gi|697136922|ref|XP_009622546.1|2.1e-7254.90PREDICTED: probable WRKY transcription factor 14 [Nicotiana tomentosiformis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR003657WRKY_dom
Vocabulary: Molecular Function
TermDefinition
GO:0003700transcription factor activity, sequence-specific DNA binding
GO:0043565sequence-specific DNA binding
Vocabulary: Biological Process
TermDefinition
GO:0006355regulation of transcription, DNA-templated
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005667 transcription factor complex
molecular_function GO:0043565 sequence-specific DNA binding
molecular_function GO:0003700 transcription factor activity, sequence-specific DNA binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.167500.1Cucsa.167500.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainGENE3DG3DSA:2.20.25.80coord: 188..257
score: 1.0
IPR003657WRKY domainPFAMPF03106WRKYcoord: 198..255
score: 1.6
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 196..256
score: 6.0
IPR003657WRKY domainPROFILEPS50811WRKYcoord: 191..257
score: 32
IPR003657WRKY domainunknownSSF118290WRKY DNA-binding domaincoord: 194..257
score: 4.45
NoneNo IPR availablePANTHERPTHR32096FAMILY NOT NAMEDcoord: 145..365
score: 5.0
NoneNo IPR availablePANTHERPTHR32096:SF18DISEASE RESISTANCE PROTEIN-LIKE-RELATEDcoord: 145..365
score: 5.0