CSPI01G25350 (gene) Wild cucumber (PI 183967)

NameCSPI01G25350
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionWRKY protein
LocationChr1 : 20820219 .. 20823801 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTATTCTCTCTCTTCTTATGTTATGTTGCGTATGGAAGAGAAGTGGTTTTTGTGAGAGAGAAAGTCAGCGTCCAATTGCTCTCTCTATAATTCTCTCTCAAATTCTTCTTTTTTTTTTTCATAAATAAAGGGAAATACCATTTCCATTTTCTTTCTCCCTAATCTCACCGGCGACAAAAATTCTGCATGTTTTCCGATTCCTCTTCACCTGACACACTCAACTTCAAAACATGTTCTGTAGTTTGTTTGAGATGGACAATAACAATTATCAGCAAGCTGGTGATTTGACTGATGTGATCCGACTGAGTTCCGCCCCTGTCGCCGGTCATTTCTCCTCCGAATTCTCTCTCGACCCGTTTTCCGGTGACAGACAACTCTGGTCGCACCTTCCTGCTGACAATTCTTCCATGAATTTTGGAGATCCGTTGTCGTTTCCAACTCGAGATCCATTTCTTCTTCCCCATTTTTCTCCCTTCAACTTTGCTTCGGATGGCGGCGGTGAGGGTTTGGCGGCGGACGACCAGCCGTCTAATTTACTTTCCCATATGTTGCAGATCTCTCCGAGTTCTGGTGATGGTGTAATTAGTACTCCTCCATGTGAGTCGCTCGCCACAGCAGTGGGGAATTCTCCGAGGTCGACTTCGGTTCGTGGTGGCGGTGGTCTTGTGCTTCCGACCGGTGGCTCGAACTCTCTTTGTTTAATGGAGAATTCCGGGATTCAGATCTCTTCTCCGCGAAATTCCGCCAATAAAAGAAGGTAAAATCAAATCTTGGACTTCTGAAATGATAATAATAACTCAGTATATATTATGTTAATGGGTGCTTTTGATCATTCTGAGTTATAACTTGTTGGGGTTCAAATGAAATTTAAATTTTGTCTTACCTTTGAGTTTCGAAAGCTTCATGATAGTTGATTAATTAGGTACTTAAAAAAAATTATGGGTTTTAGAAAAATATGTATAGGCTTTCTCCTATAGGGATTATTTTAGGATATTGCTGGGTTCAAACTCTCTAGTCCAACAAATGGAAAATAGGAGCATAAAGCTATGACTTTTAAGACATAATTCTCTTATGACTGAATGAACTTTGTCTTTACTATATTTATAGAAGGTTCAAAGGGTGGAGAAAACATTTTTTTTCCTTAAATATACCTTGGAGAAATGTAAACTCTCGTAGTCTTCGATCACATTTCAATGTCTACTTTAATGAAAAAATAAAACTAAAAGGAAAACAAATCAAATTTAGACTATGTTCTAAATAAAATAACAGTAACTAGCTATTTCAAGACCATATACATATCTATGTACAGACTCTATCTCATTTTTAGAGTTTAAGTTAATAAACTATATTAAGATTGTTGTAGATAAATTAGCATTAAAATTTTAAATATTTGGTACCTCTAATTAACCACAAGTTTAATTAGAGTGGAATAACTAAGAAAAATTCTACCGCCTCTCCCATAGTTAACTAAAACTCCATCTGATCATAACAATTTAACTTTCCTTTTGGTTTTATTAAAAAAAAAAAGAAGAAGAAATAAAGAAGAGAAAAACAAGAGAATTGAACTCCAAATTACAAAATTGTTGCTTCAAACAAAAACGCTATTAAAATGAATTCCTACATGAATGAATTAACTAATGTGGTTGGTTGTTCTCAGGAAGAGCCAAGTGAAGAAGGTGGTGTGTATTCCGGCACCGGCACCGGCGAACAGTCGATCAAGCAGCGGCGAAGTGGTTCCTTCCGATCTATGGGCATGGAGGAAGTACGGACAAAAGCCCATCAAAGGATCCCCATATCCAAGGTCAACTTTCTTCAAAACTTTAATCTTATTATTACTTTTAGGTTAAATATATTATCATTTGGTTCCCTCTCTTCCTTCCATAAATCCTAAATTTAGTCCTCAAATTGTTTAAATAATATATAACAACAATAATAACATTCATGAAATAATTATATATAATAGGAGAATGTATTCCTAAATTTATAACAACAAAAAAAAAAAAAGTTGCTAATAGATAATAAAACGTCTTTAAAAAAATCAACTAAATGAACAAACAAGAGAAGTATGTTAAGATTTCTGCATAGACACTATATAAAATTCAAAATCTGAAAGTGTTGGTAGATTAAGTTGAATTGAAATGATTTTAACTGTCTAAATTGATCATTGTTCATCCTAACTTTCCAAGTCAAAAAGATTTTGACCTCAAATATTAATTTTTTTTTCTAATTTTCTTTGGTAATTTTCAATTAATTTATTTTCTACTTAAACATGAGAGAGAGAAAAAAAGAGTTTTGTTTTCCAGAAATCAATGCCAAATGCTGCAAGTTTTTGTACCTCTTTCTCTCTCTCTCTAGCTGACAAGAAATTGACTCCCAATAATTTCATGAATATCTCACTCATGATCATTTACTCATCATTTCTTCAAGTGGGCTTTAAAGTTGGGGCAGAGAAATCATAATATTTTCTAAACTTCTATATATACTAATATATTTTTTATTTATAGAAATACTATATAGTTTTGAGATGTTCTTTATTTCAATTTATGGTATTTAATTTGTTCTAGTCCAATTCTTTAGTTGTTGGTGTTAATCAAAACATGTGTTTTGTGTTCCATTTGTATTTTTCTTCCCTTATTAATGATGGTTGACTTTTCAAAATTAAATGTTATTAGTAGATAAATGGTAATTCTTTTTTTTTTCCTTTTAATTACTTTGGATGTGATTTTTAAAAACTAACTAGCTTGTTTAACAATTCCTTTCTTATGTTGTAGAAGTATTTACATTTTTTCCAAAACCCTCAAATCTTTCATATACTTAATTCTCATTAATGTTGATCATCTTCATTATATATATATAGTATATGCCTTATAGTTCTTTTATGCCTTTCTTTCTCTTCTCTTTTCTTTCAGGGGATATTATAGGTGCAGTAGTTCCAAGGGCTGCTCCGCTCGAAAGCAGGTCGAGCGAAGTCGTACTAACCCCAACATGCTTGTCATCACCTACACTTCGGAGCACAACCACCCATGGCCCACTCAACGCAATGCCCTTGCAGGTTCTACTCGTTCCCATCCTTCACGAACCACCACCGCTACCACCGCTAATAAAACAAGCCCGAAACATGAGCGCATTAACGACATCCTGCCAACCACAACAATAAAGGAGGAAGAGATGGATGAAGACCAAATGACAGATAAAGCAACAACAACAAATGATAATCAGGAGGAGGGAGATCATCAAGATTTCCCTTATGACTTAATTTTCACTGAATTTGCAGATCAAATGAACGACCTCGAGCACGACAACAACCACGACCATAATCATCATCTTTCAGCAGACCCTCTGAACAACAATATGTTGATGTTTAGTAACCATGGATTTAGTGCAGGAAATGGAGAAGGCAGTAAAGATCCATTTTTGGAGCTCTATGATTGGGCAGAGAATTCAAGTGGAAGTTTGTTCAAAGAAGCCAAGGGAGGTTGACGGAGGGTAATTATCAAACCACAGTTTAAAAAGAGCTTTATATATAAAAATTAATAACAAAACTATATATATATAAAATTTTAAACATTATATACACATGCAG

mRNA sequence

ATGTTCTGTAGTTTGTTTGAGATGGACAATAACAATTATCAGCAAGCTGGTGATTTGACTGATGTGATCCGACTGAGTTCCGCCCCTGTCGCCGGTCATTTCTCCTCCGAATTCTCTCTCGACCCGTTTTCCGGTGACAGACAACTCTGGTCGCACCTTCCTGCTGACAATTCTTCCATGAATTTTGGAGATCCGTTGTCGTTTCCAACTCGAGATCCATTTCTTCTTCCCCATTTTTCTCCCTTCAACTTTGCTTCGGATGGCGGCGGTGAGGGTTTGGCGGCGGACGACCAGCCGTCTAATTTACTTTCCCATATGTTGCAGATCTCTCCGAGTTCTGGTGATGGTGTAATTAGTACTCCTCCATGTGAGTCGCTCGCCACAGCAGTGGGGAATTCTCCGAGGTCGACTTCGGTTCGTGGTGGCGGTGGTCTTGTGCTTCCGACCGGTGGCTCGAACTCTCTTTGTTTAATGGAGAATTCCGGGATTCAGATCTCTTCTCCGCGAAATTCCGCCAATAAAAGAAGGAAGAGCCAAGTGAAGAAGGTGGTGTGTATTCCGGCACCGGCACCGGCGAACAGTCGATCAAGCAGCGGCGAAGTGGTTCCTTCCGATCTATGGGCATGGAGGAAGTACGGACAAAAGCCCATCAAAGGATCCCCATATCCAAGGGGATATTATAGGTGCAGTAGTTCCAAGGGCTGCTCCGCTCGAAAGCAGGTCGAGCGAAGTCGTACTAACCCCAACATGCTTGTCATCACCTACACTTCGGAGCACAACCACCCATGGCCCACTCAACGCAATGCCCTTGCAGGTTCTACTCGTTCCCATCCTTCACGAACCACCACCGCTACCACCGCTAATAAAACAAGCCCGAAACATGAGCGCATTAACGACATCCTGCCAACCACAACAATAAAGGAGGAAGAGATGGATGAAGACCAAATGACAGATAAAGCAACAACAACAAATGATAATCAGGAGGAGGGAGATCATCAAGATTTCCCTTATGACTTAATTTTCACTGAATTTGCAGATCAAATGAACGACCTCGAGCACGACAACAACCACGACCATAATCATCATCTTTCAGCAGACCCTCTGAACAACAATATGTTGATGTTTAGTAACCATGGATTTAGTGCAGGAAATGGAGAAGGCAGTAAAGATCCATTTTTGGAGCTCTATGATTGGGCAGAGAATTCAAGTGGAAGTTTGTTCAAAGAAGCCAAGGGAGGTTGA

Coding sequence (CDS)

ATGTTCTGTAGTTTGTTTGAGATGGACAATAACAATTATCAGCAAGCTGGTGATTTGACTGATGTGATCCGACTGAGTTCCGCCCCTGTCGCCGGTCATTTCTCCTCCGAATTCTCTCTCGACCCGTTTTCCGGTGACAGACAACTCTGGTCGCACCTTCCTGCTGACAATTCTTCCATGAATTTTGGAGATCCGTTGTCGTTTCCAACTCGAGATCCATTTCTTCTTCCCCATTTTTCTCCCTTCAACTTTGCTTCGGATGGCGGCGGTGAGGGTTTGGCGGCGGACGACCAGCCGTCTAATTTACTTTCCCATATGTTGCAGATCTCTCCGAGTTCTGGTGATGGTGTAATTAGTACTCCTCCATGTGAGTCGCTCGCCACAGCAGTGGGGAATTCTCCGAGGTCGACTTCGGTTCGTGGTGGCGGTGGTCTTGTGCTTCCGACCGGTGGCTCGAACTCTCTTTGTTTAATGGAGAATTCCGGGATTCAGATCTCTTCTCCGCGAAATTCCGCCAATAAAAGAAGGAAGAGCCAAGTGAAGAAGGTGGTGTGTATTCCGGCACCGGCACCGGCGAACAGTCGATCAAGCAGCGGCGAAGTGGTTCCTTCCGATCTATGGGCATGGAGGAAGTACGGACAAAAGCCCATCAAAGGATCCCCATATCCAAGGGGATATTATAGGTGCAGTAGTTCCAAGGGCTGCTCCGCTCGAAAGCAGGTCGAGCGAAGTCGTACTAACCCCAACATGCTTGTCATCACCTACACTTCGGAGCACAACCACCCATGGCCCACTCAACGCAATGCCCTTGCAGGTTCTACTCGTTCCCATCCTTCACGAACCACCACCGCTACCACCGCTAATAAAACAAGCCCGAAACATGAGCGCATTAACGACATCCTGCCAACCACAACAATAAAGGAGGAAGAGATGGATGAAGACCAAATGACAGATAAAGCAACAACAACAAATGATAATCAGGAGGAGGGAGATCATCAAGATTTCCCTTATGACTTAATTTTCACTGAATTTGCAGATCAAATGAACGACCTCGAGCACGACAACAACCACGACCATAATCATCATCTTTCAGCAGACCCTCTGAACAACAATATGTTGATGTTTAGTAACCATGGATTTAGTGCAGGAAATGGAGAAGGCAGTAAAGATCCATTTTTGGAGCTCTATGATTGGGCAGAGAATTCAAGTGGAAGTTTGTTCAAAGAAGCCAAGGGAGGTTGA
BLAST of CSPI01G25350 vs. Swiss-Prot
Match: WRK14_ARATH (Probable WRKY transcription factor 14 OS=Arabidopsis thaliana GN=WRKY14 PE=2 SV=2)

HSP 1 Score: 218.8 bits (556), Expect = 1.1e-55
Identity = 172/405 (42.47%), Postives = 217/405 (53.58%), Query Frame = 1

Query: 3   CSLFEM-DNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLW----SHLPADN 62
           CS+ E+ D  N+Q  GDLTDV+R     + GH      L P +    +W    SH     
Sbjct: 2   CSVSELLDMENFQ--GDLTDVVR----GIGGHV-----LSPETPPSNIWPLPLSHPTPSP 61

Query: 63  SSMN---FGDPLSFPTRDPFLLPHFSP------FNFASDGGGE----------GLAADDQ 122
           S +N   FGDP  F + D  LL   +       F+   D               +  +D 
Sbjct: 62  SDLNINPFGDP--FVSMDDPLLQELNSITNSGYFSTVGDNNNNIHNNNGFLVPKVFEEDH 121

Query: 123 PSNLLSHMLQISPSSGDGVISTPPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLM 182
             +  S   +I  S  + +  + PC S A +      + +    G + + T    +  L+
Sbjct: 122 IKSQCSIFPRIRISHSNIIHDSSPCNSPAMSAHVVAAAAAASPRGIINVDTNSPRNCLLV 181

Query: 183 E----NSGIQISSPRNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQ 242
           +    +S IQISSPRN   KRRKSQ KKVVCIPAPA  NSR SSGEVVPSDLWAWRKYGQ
Sbjct: 182 DGTTFSSQIQISSPRNLGLKRRKSQAKKVVCIPAPAAMNSR-SSGEVVPSDLWAWRKYGQ 241

Query: 243 KPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGST 302
           KPIKGSP+PRGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWP QRNALAGST
Sbjct: 242 KPIKGSPFPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRNALAGST 301

Query: 303 RSHPSRTTTATTANKTSPKHERIND---------ILPTTTIK---------EEEMDEDQM 356
           R   S T++++  N + P    +N           LP++T           ++E  +D  
Sbjct: 302 R---SSTSSSSNPNPSKPSTANVNSSSIGSQNTIYLPSSTTPPPTLSSSAIKDERGDDME 361

BLAST of CSPI01G25350 vs. Swiss-Prot
Match: WRK35_ARATH (Probable WRKY transcription factor 35 OS=Arabidopsis thaliana GN=WRKY35 PE=2 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 1.1e-53
Identity = 181/439 (41.23%), Postives = 223/439 (50.80%), Query Frame = 1

Query: 11  NNYQQAGDLTDVIR--------LSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSMN- 70
           +N+Q  GDLTDV+R         S  P  G   S  S  P S    L    P+  +S + 
Sbjct: 2   DNFQ--GDLTDVVRGIGSGHVSPSPGPPEGPSPSSMSPPPTS---DLHVEFPSAATSASC 61

Query: 71  ----FGDPLSFPTRDPFLLPHFSPFNFASDGGGEGLAA-------DDQPSNLLSHMLQIS 130
               FGDP     +DP +    S  + A D       A       DD   +  S   +I 
Sbjct: 62  LANPFGDPF-VSMKDPLIHLPASYISGAGDNKSNKSFAIFPKIFEDDHIKSQCSVFPRIK 121

Query: 131 PSSGDGVISTPPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMEN------SGIQ 190
            S  + +     C S A  V ++  + S  G   +       N L +  N      S +Q
Sbjct: 122 ISQSNNIHDASTCNSPAITVSSAAVAASPWGMINVNTTNSPRNCLLVDNNNNTSSCSQVQ 181

Query: 191 ISS-PRNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYP 250
           ISS PRN   KRRKSQ KKVVCIPAPA  NSRS SGEVVPSDLWAWRKYGQKPIKGSPYP
Sbjct: 182 ISSSPRNLGIKRRKSQAKKVVCIPAPAAMNSRS-SGEVVPSDLWAWRKYGQKPIKGSPYP 241

Query: 251 RGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPS---- 310
           RGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTRS  S    
Sbjct: 242 RGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSSSSLN 301

Query: 311 -RTTTATTANKTSP----------KHERINDILPTTTIKEEEMDEDQMTDKATTTNDNQE 370
             + ++T A  TSP          K E  N  LP+++            D A    +N E
Sbjct: 302 PSSKSSTAAATTSPSSRVFQNNSSKDEPNNSNLPSSSTHPP-------FDAAAIKEENVE 361

Query: 371 E-GDHQDFPYDLIFTEFADQMNDLEHDNNHDHNHHLSADPLNNNML-MFSNHGFSAGNGE 403
           E  +  +F Y+ +   +  ++  L+   +   +     D L  + L M  +H    GN E
Sbjct: 362 ERQEKMEFDYNDVENTYRPEL--LQEFQHQPEDFFADLDELEGDSLTMLLSHSSGGGNME 421

BLAST of CSPI01G25350 vs. Swiss-Prot
Match: WRK22_ARATH (WRKY transcription factor 22 OS=Arabidopsis thaliana GN=WRKY22 PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.5e-31
Identity = 89/195 (45.64%), Postives = 113/195 (57.95%), Query Frame = 1

Query: 148 PTGGSNSLCLMENSGIQISSP---RNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPS 207
           P      L L  +SG   S P     S +KRRK Q KKV  + A           E + S
Sbjct: 80  PQNQKRPLSLSASSGSVTSKPSGSNTSRSKRRKIQHKKVCHVAA-----------EALNS 139

Query: 208 DLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWP 267
           D+WAWRKYGQKPIKGSPYPRGYYRCS+SKGC ARKQVER+R++P M ++TYT+EHNHP P
Sbjct: 140 DVWAWRKYGQKPIKGSPYPRGYYRCSTSKGCLARKQVERNRSDPKMFIVTYTAEHNHPAP 199

Query: 268 TQRNALAGSTRSHPS------RTTTATTANKTSPKHERINDILPT---TTIKEEEMDEDQ 327
           T RN+LAGSTR  PS        TT      +SP       +LP      + + + +ED 
Sbjct: 200 THRNSLAGSTRQKPSDQQTSKSPTTTIATYSSSPVTSADEFVLPVEDHLAVGDLDGEEDL 259

Query: 328 MTDKATTTNDNQEEG 331
           ++   T  +D+  +G
Sbjct: 260 LSLSDTVVSDDFFDG 263

BLAST of CSPI01G25350 vs. Swiss-Prot
Match: WRK16_ARATH (Probable WRKY transcription factor 16 OS=Arabidopsis thaliana GN=WRKY16 PE=2 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 4.3e-31
Identity = 80/155 (51.61%), Postives = 102/155 (65.81%), Query Frame = 1

Query: 171  SANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCS 230
            S ++R+ ++ K+VVC+       SRSS       DLW WRKYGQKPIK SPYPR YYRC+
Sbjct: 1157 SKSRRKNNKEKRVVCV---VDRGSRSS-------DLWVWRKYGQKPIKSSPYPRSYYRCA 1216

Query: 231  SSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSR---TTTATTA 290
            SSKGC ARKQVERSRT+PN+ VITY SEHNHP+PT RN LAGSTRS  S+    TT+ ++
Sbjct: 1217 SSKGCFARKQVERSRTDPNVSVITYISEHNHPFPTLRNTLAGSTRSSSSKCSDVTTSASS 1276

Query: 291  NKTSPKHERINDILPTT---------TIKEEEMDE 314
              +  K       LP++          +KEE+M++
Sbjct: 1277 TVSQDKEGPDKSHLPSSPASPPYAAMVVKEEDMEQ 1301

BLAST of CSPI01G25350 vs. Swiss-Prot
Match: WRK65_ARATH (Probable WRKY transcription factor 65 OS=Arabidopsis thaliana GN=WRKY65 PE=2 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 4.8e-30
Identity = 77/154 (50.00%), Postives = 93/154 (60.39%), Query Frame = 1

Query: 161 SGIQISSPRNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGS 220
           +G++     +S  + R+S  K+VV +P      SR       PSD WAWRKYGQKPIKGS
Sbjct: 31  NGMKALISSHSPKRSRRSVEKRVVNVPMKEMEGSRHKGDTTPPSDSWAWRKYGQKPIKGS 90

Query: 221 PYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSR 280
           PYPRGYYRCSS+KGC ARKQVERSR +P M++ITYTSEHNHPWP     L  STR+ P  
Sbjct: 91  PYPRGYYRCSSTKGCPARKQVERSRDDPTMILITYTSEHNHPWP-----LTSSTRNGPK- 150

Query: 281 TTTATTANKTSPKHERINDILPTTTIKEEEMDED 315
                   K  PK E      P   ++ E  +ED
Sbjct: 151 -------PKPEPKPE------PEPEVEPEAEEED 165

BLAST of CSPI01G25350 vs. TrEMBL
Match: A0A0A0LYP6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G547510 PE=4 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 1.2e-237
Identity = 413/413 (100.00%), Postives = 413/413 (100.00%), Query Frame = 1

Query: 1   MFCSLFEMDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSM 60
           MFCSLFEMDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSM
Sbjct: 1   MFCSLFEMDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSM 60

Query: 61  NFGDPLSFPTRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSSGDGVIST 120
           NFGDPLSFPTRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSSGDGVIST
Sbjct: 61  NFGDPLSFPTRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSSGDGVIST 120

Query: 121 PPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNSANKRRKSQV 180
           PPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNSANKRRKSQV
Sbjct: 121 PPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNSANKRRKSQV 180

Query: 181 KKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQ 240
           KKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQ
Sbjct: 181 KKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQ 240

Query: 241 VERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKHERINDI 300
           VERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKHERINDI
Sbjct: 241 VERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKHERINDI 300

Query: 301 LPTTTIKEEEMDEDQMTDKATTTNDNQEEGDHQDFPYDLIFTEFADQMNDLEHDNNHDHN 360
           LPTTTIKEEEMDEDQMTDKATTTNDNQEEGDHQDFPYDLIFTEFADQMNDLEHDNNHDHN
Sbjct: 301 LPTTTIKEEEMDEDQMTDKATTTNDNQEEGDHQDFPYDLIFTEFADQMNDLEHDNNHDHN 360

Query: 361 HHLSADPLNNNMLMFSNHGFSAGNGEGSKDPFLELYDWAENSSGSLFKEAKGG 414
           HHLSADPLNNNMLMFSNHGFSAGNGEGSKDPFLELYDWAENSSGSLFKEAKGG
Sbjct: 361 HHLSADPLNNNMLMFSNHGFSAGNGEGSKDPFLELYDWAENSSGSLFKEAKGG 413

BLAST of CSPI01G25350 vs. TrEMBL
Match: A0A059A3C8_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_K01971 PE=4 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.0e-63
Identity = 199/465 (42.80%), Postives = 252/465 (54.19%), Query Frame = 1

Query: 3   CSLFEMDN--NNYQQAGDLTDVIRLSSAPVAGH---FSSEFSLDPFSGDRQLWSHLPADN 62
           CSLF+ D   +NY Q GDLTD+IR   + + G     ++ +            S +  ++
Sbjct: 2   CSLFKQDQIMDNYHQ-GDLTDIIRAGGSGMVGEPAVATNTWQFPSMDNPESFSSAMDENS 61

Query: 63  SSMNFGDPLSFPTRDPFL-----------------LPHFSPFNFASDGGGEGLAA----D 122
           +  +FGDP S   RDP L                  P F      S       AA    D
Sbjct: 62  NPHSFGDPFS-SMRDPLLHNINMPNSGFLKPAEMVSPSFDSTGDFSSNSSVMYAAPPIED 121

Query: 123 D--QPSNLLSHMLQISPSSGDGVISTPPCESLATAVGNSPRSTSVRGGGGLVLPTG---- 182
           D  +P N+ + MLQI+P+     +   PC S   A   SP           ++P+     
Sbjct: 122 DLKRPCNIFTRMLQITPNK----LPISPCGSPVMAA--SPPGIQ----NPSIIPSDMISL 181

Query: 183 GSNSLCLMENSGIQISSPRNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWR 242
             + +CL++NS +QISSPRN   KRRKSQ KKVVCIPAPA ANSR+  GEVVPSDLWAWR
Sbjct: 182 NGSKVCLVDNSAMQISSPRNPGIKRRKSQAKKVVCIPAPAAANSRAG-GEVVPSDLWAWR 241

Query: 243 KYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNAL 302
           KYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNAL
Sbjct: 242 KYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNAL 301

Query: 303 AGSTRS-HPSRTTTATTANK--------TSPKHERINDILPTT----------------- 362
           AGSTRS  PS+ +  T+ N          SPK E+  D+  +T                 
Sbjct: 302 AGSTRSTQPSKNSNVTSKNSPNSQTHKHISPKEEQKEDVNDSTIPMEPSGGLAAGATAGA 361

Query: 363 TIKEEEMDEDQMTDKATTTNDNQEEGDHQDFPYDLIFTEFADQMNDLEHDNNHDHNHHLS 406
           +IKEE  +E++M        D Q E D ++F   L        ++  + D+       L 
Sbjct: 362 SIKEE--NENKM--------DRQMEFDDEEFGDGLGHGYRPSMLDANQTDDFFADLGELE 421

BLAST of CSPI01G25350 vs. TrEMBL
Match: M5WIC8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004535mg PE=4 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 3.9e-63
Identity = 190/425 (44.71%), Postives = 237/425 (55.76%), Query Frame = 1

Query: 21  DVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSMNFGDP--LSFPTRDPFLLPH 80
           D+   SS  ++ + SS+ +++  S D        A  +S  FG    +  P   P   PH
Sbjct: 93  DISSSSSFFISPNSSSDMNINITSVDE-------AGGTSSTFGGAHIVLAPPPPP---PH 152

Query: 81  FSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSSGDGVISTP-PCESLATAVGNSPRST 140
               +    GG        +P N+ S MLQISPS+   V +   P    ATAV +SPR  
Sbjct: 153 HHHLHHHQQGGVVDGDPIKRPCNIFSRMLQISPSAKVPVTACDSPLLGAATAV-SSPRGI 212

Query: 141 SVRGGGGLVLPTGGSNSL----------CLMENSGIQISSPRNSANKRRKSQVKKVVCIP 200
           S    G +     GS+ +          CL+EN+G+QISSPRN   KRRKSQ KKVVCIP
Sbjct: 213 SKAVSGSISGAMVGSDMIINGNSSSGKGCLLENTGVQISSPRNPGIKRRKSQAKKVVCIP 272

Query: 201 APAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTN 260
           APA ANSR  +GEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRT+
Sbjct: 273 APAAANSRP-TGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTD 332

Query: 261 PNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKHERINDILPTTTIK 320
           PNMLVITYTSEHNHPWPTQRNALAGSTRS PS+++  +  +  S          PT   +
Sbjct: 333 PNMLVITYTSEHNHPWPTQRNALAGSTRSQPSKSSAGSKTSSNSSHQPHQKPASPTKEEQ 392

Query: 321 EEEMDEDQMTDKATTTN------------DNQEEGDHQ-DFPYDLIFTEFADQMNDLEHD 380
            E M+++       T              + Q E DHQ  FPY     +  DQ N   H+
Sbjct: 393 NETMNDNNHVSPVITAGSASTVKEEFEDIEKQLELDHQAGFPYRPSMPD--DQSNQSHHN 452

Query: 381 NNHDHN-HHLSADPLNNNMLMFSNHGFSAGNGEGSK----DPFLELYDWA----ENSSGS 411
            +   +   + ADPLN   L+FS    +    +GSK    DPF  L+DW+     N++ +
Sbjct: 453 EDFFADLGEIEADPLN---LLFSQCFTADEQQKGSKAAALDPF-NLFDWSGDQNTNNNTT 499

BLAST of CSPI01G25350 vs. TrEMBL
Match: V4U7V4_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10014984mg PE=4 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 3.9e-63
Identity = 215/512 (41.99%), Postives = 254/512 (49.61%), Query Frame = 1

Query: 3   CSLFEMDNNNYQQAGDLTDVIRLSSAPVAGHFS-----SEFSLDPFSGDRQLWSHLP--- 62
           CS F ++  NYQ  GDLTD+IR     +  + S     S  S    S    L + LP   
Sbjct: 2   CSFFGVEMENYQ--GDLTDIIRAGGRQIGSNNSASVSASSSSASVVSASDHLNNPLPTWQ 61

Query: 63  ------------ADNSSMNFGDPLSFPTRDPFLL-------------PHFSPFNFASDGG 122
                        DN    FGDP S  TRDP L              P   P +   D  
Sbjct: 62  FPSDPLSFCSSIDDNRENLFGDPFS-NTRDPLLHELNIPNSSGYYAPPPLPPADRVVDHE 121

Query: 123 G----------------EGLAADDQ---PSNLLSHMLQISPSSGDGVI--STPPCESLAT 182
                             GL  +D+   P N+ S  +QISPSS       +  PCES   
Sbjct: 122 AATAASNINIGRRHHHQRGLFHEDELKSPCNIFSR-IQISPSSQSPKQQPAVSPCESPVI 181

Query: 183 AVGNSPRS--TSVRGGGGLVLPTGGSNSLCLMENSG-IQISSPRNSANKRRKSQVKKVVC 242
           A   SPR   T V     ++     S S CL++N+G  QISSPRN   KRRKSQ KKVVC
Sbjct: 182 AAAASPRGIKTPVVVASDMINANSTSKS-CLVDNTGPAQISSPRNLGIKRRKSQAKKVVC 241

Query: 243 IPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSR 302
           IPAPA ANSRS SGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSR
Sbjct: 242 IPAPAAANSRS-SGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSR 301

Query: 303 TNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTA---------------TTANKT 362
           T+PNMLVITYTSEHNHPWPTQRNALAGSTRS PS+   A               TT N +
Sbjct: 302 TDPNMLVITYTSEHNHPWPTQRNALAGSTRSQPSKANAAAKGSVSPSSHQPPQNTTTNNS 361

Query: 363 SPKHERINDILPTTTIKEEEMDEDQMTDKATTTNDNQEE-----GDHQDFPYDLIFTEFA 414
           + K         TTT+     + +     +T+  +  EE      D Q    DL   EF+
Sbjct: 362 NTKENVKESSNDTTTLSPSNNNFNAAPSSSTSVKEEIEEIEKPIDDDQMEGADL--DEFS 421

BLAST of CSPI01G25350 vs. TrEMBL
Match: A0A0D2UKC0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_011G006600 PE=4 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.5e-62
Identity = 186/381 (48.82%), Postives = 212/381 (55.64%), Query Frame = 1

Query: 6   FEMDNNNYQQAGDLTDVIRLSSAPVAGHFSS----------EF-SLDPFSGDRQLWSHLP 65
           F ++  NYQ  GDL D++R S+ P   HFS           EF S DP S      S + 
Sbjct: 3   FGLEMENYQ--GDLNDIVRASAEPA--HFSDMVPQLSSWQQEFPSSDPLSFSS---SAME 62

Query: 66  ADNSSMNFGDPLSFPTRDPFLLPHFSPFNFASDGGGE-----------------GLAADD 125
            D S+  FGDP S   RDP LL      N    G                    G     
Sbjct: 63  DDYSTNTFGDPFS-AIRDPLLL--LQELNVVGTGSSSTYFNNSPNSTNDHHHMGGFDDMT 122

Query: 126 QPSNLLSHMLQISPSSGDGVISTPPCESLATAVGNSPRSTSVRGGGG----LVLPTGGSN 185
            P N+ S  +QIS SS       PPC+    A  ++       G GG     V P+   N
Sbjct: 123 SPCNIFSR-IQISHSSNP----LPPCDPPRMAAAST-------GLGGHKAPAVFPSDMVN 182

Query: 186 SL-CLMENSG------IQISSPRNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDL 245
           +  CL++N+       +QISSPRN   KRRKSQ KKVVCIPAPA ANSRS SGEVVPSDL
Sbjct: 183 AKSCLIDNTAAAASVPVQISSPRNLGIKRRKSQGKKVVCIPAPAAANSRS-SGEVVPSDL 242

Query: 246 WAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQ 305
           WAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWPTQ
Sbjct: 243 WAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQ 302

Query: 306 RNALAGSTRSHPSRTTTATTANKTSPKHERINDILPTTTIKEEEMDEDQMTDKA------ 340
           RNALAGSTRS PS+      AN T  K    +D  P  T KE   +E +M D A      
Sbjct: 303 RNALAGSTRSQPSK----PNANNTGSKMGSPSDSEPQKTTKETIKEETEMDDMAKQQLDI 356

BLAST of CSPI01G25350 vs. TAIR10
Match: AT1G30650.1 (AT1G30650.1 WRKY DNA-binding protein 14)

HSP 1 Score: 218.8 bits (556), Expect = 6.4e-57
Identity = 172/405 (42.47%), Postives = 217/405 (53.58%), Query Frame = 1

Query: 3   CSLFEM-DNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLW----SHLPADN 62
           CS+ E+ D  N+Q  GDLTDV+R     + GH      L P +    +W    SH     
Sbjct: 2   CSVSELLDMENFQ--GDLTDVVR----GIGGHV-----LSPETPPSNIWPLPLSHPTPSP 61

Query: 63  SSMN---FGDPLSFPTRDPFLLPHFSP------FNFASDGGGE----------GLAADDQ 122
           S +N   FGDP  F + D  LL   +       F+   D               +  +D 
Sbjct: 62  SDLNINPFGDP--FVSMDDPLLQELNSITNSGYFSTVGDNNNNIHNNNGFLVPKVFEEDH 121

Query: 123 PSNLLSHMLQISPSSGDGVISTPPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLM 182
             +  S   +I  S  + +  + PC S A +      + +    G + + T    +  L+
Sbjct: 122 IKSQCSIFPRIRISHSNIIHDSSPCNSPAMSAHVVAAAAAASPRGIINVDTNSPRNCLLV 181

Query: 183 E----NSGIQISSPRNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQ 242
           +    +S IQISSPRN   KRRKSQ KKVVCIPAPA  NSR SSGEVVPSDLWAWRKYGQ
Sbjct: 182 DGTTFSSQIQISSPRNLGLKRRKSQAKKVVCIPAPAAMNSR-SSGEVVPSDLWAWRKYGQ 241

Query: 243 KPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGST 302
           KPIKGSP+PRGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWP QRNALAGST
Sbjct: 242 KPIKGSPFPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRNALAGST 301

Query: 303 RSHPSRTTTATTANKTSPKHERIND---------ILPTTTIK---------EEEMDEDQM 356
           R   S T++++  N + P    +N           LP++T           ++E  +D  
Sbjct: 302 R---SSTSSSSNPNPSKPSTANVNSSSIGSQNTIYLPSSTTPPPTLSSSAIKDERGDDME 361

BLAST of CSPI01G25350 vs. TAIR10
Match: AT2G34830.1 (AT2G34830.1 WRKY DNA-binding protein 35)

HSP 1 Score: 212.2 bits (539), Expect = 6.0e-55
Identity = 181/439 (41.23%), Postives = 223/439 (50.80%), Query Frame = 1

Query: 11  NNYQQAGDLTDVIR--------LSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSMN- 70
           +N+Q  GDLTDV+R         S  P  G   S  S  P S    L    P+  +S + 
Sbjct: 2   DNFQ--GDLTDVVRGIGSGHVSPSPGPPEGPSPSSMSPPPTS---DLHVEFPSAATSASC 61

Query: 71  ----FGDPLSFPTRDPFLLPHFSPFNFASDGGGEGLAA-------DDQPSNLLSHMLQIS 130
               FGDP     +DP +    S  + A D       A       DD   +  S   +I 
Sbjct: 62  LANPFGDPF-VSMKDPLIHLPASYISGAGDNKSNKSFAIFPKIFEDDHIKSQCSVFPRIK 121

Query: 131 PSSGDGVISTPPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMEN------SGIQ 190
            S  + +     C S A  V ++  + S  G   +       N L +  N      S +Q
Sbjct: 122 ISQSNNIHDASTCNSPAITVSSAAVAASPWGMINVNTTNSPRNCLLVDNNNNTSSCSQVQ 181

Query: 191 ISS-PRNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYP 250
           ISS PRN   KRRKSQ KKVVCIPAPA  NSRS SGEVVPSDLWAWRKYGQKPIKGSPYP
Sbjct: 182 ISSSPRNLGIKRRKSQAKKVVCIPAPAAMNSRS-SGEVVPSDLWAWRKYGQKPIKGSPYP 241

Query: 251 RGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPS---- 310
           RGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTRS  S    
Sbjct: 242 RGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSSSSLN 301

Query: 311 -RTTTATTANKTSP----------KHERINDILPTTTIKEEEMDEDQMTDKATTTNDNQE 370
             + ++T A  TSP          K E  N  LP+++            D A    +N E
Sbjct: 302 PSSKSSTAAATTSPSSRVFQNNSSKDEPNNSNLPSSSTHPP-------FDAAAIKEENVE 361

Query: 371 E-GDHQDFPYDLIFTEFADQMNDLEHDNNHDHNHHLSADPLNNNML-MFSNHGFSAGNGE 403
           E  +  +F Y+ +   +  ++  L+   +   +     D L  + L M  +H    GN E
Sbjct: 362 ERQEKMEFDYNDVENTYRPEL--LQEFQHQPEDFFADLDELEGDSLTMLLSHSSGGGNME 421

BLAST of CSPI01G25350 vs. TAIR10
Match: AT4G01250.1 (AT4G01250.1 WRKY family transcription factor)

HSP 1 Score: 138.7 bits (348), Expect = 8.4e-33
Identity = 89/195 (45.64%), Postives = 113/195 (57.95%), Query Frame = 1

Query: 148 PTGGSNSLCLMENSGIQISSP---RNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPS 207
           P      L L  +SG   S P     S +KRRK Q KKV  + A           E + S
Sbjct: 80  PQNQKRPLSLSASSGSVTSKPSGSNTSRSKRRKIQHKKVCHVAA-----------EALNS 139

Query: 208 DLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWP 267
           D+WAWRKYGQKPIKGSPYPRGYYRCS+SKGC ARKQVER+R++P M ++TYT+EHNHP P
Sbjct: 140 DVWAWRKYGQKPIKGSPYPRGYYRCSTSKGCLARKQVERNRSDPKMFIVTYTAEHNHPAP 199

Query: 268 TQRNALAGSTRSHPS------RTTTATTANKTSPKHERINDILPT---TTIKEEEMDEDQ 327
           T RN+LAGSTR  PS        TT      +SP       +LP      + + + +ED 
Sbjct: 200 THRNSLAGSTRQKPSDQQTSKSPTTTIATYSSSPVTSADEFVLPVEDHLAVGDLDGEEDL 259

Query: 328 MTDKATTTNDNQEEG 331
           ++   T  +D+  +G
Sbjct: 260 LSLSDTVVSDDFFDG 263

BLAST of CSPI01G25350 vs. TAIR10
Match: AT5G45050.1 (AT5G45050.1 Disease resistance protein (TIR-NBS-LRR class))

HSP 1 Score: 137.1 bits (344), Expect = 2.4e-32
Identity = 80/155 (51.61%), Postives = 102/155 (65.81%), Query Frame = 1

Query: 171  SANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCS 230
            S ++R+ ++ K+VVC+       SRSS       DLW WRKYGQKPIK SPYPR YYRC+
Sbjct: 1157 SKSRRKNNKEKRVVCV---VDRGSRSS-------DLWVWRKYGQKPIKSSPYPRSYYRCA 1216

Query: 231  SSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSR---TTTATTA 290
            SSKGC ARKQVERSRT+PN+ VITY SEHNHP+PT RN LAGSTRS  S+    TT+ ++
Sbjct: 1217 SSKGCFARKQVERSRTDPNVSVITYISEHNHPFPTLRNTLAGSTRSSSSKCSDVTTSASS 1276

Query: 291  NKTSPKHERINDILPTT---------TIKEEEMDE 314
              +  K       LP++          +KEE+M++
Sbjct: 1277 TVSQDKEGPDKSHLPSSPASPPYAAMVVKEEDMEQ 1301

BLAST of CSPI01G25350 vs. TAIR10
Match: AT1G29280.1 (AT1G29280.1 WRKY DNA-binding protein 65)

HSP 1 Score: 133.7 bits (335), Expect = 2.7e-31
Identity = 77/154 (50.00%), Postives = 93/154 (60.39%), Query Frame = 1

Query: 161 SGIQISSPRNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGS 220
           +G++     +S  + R+S  K+VV +P      SR       PSD WAWRKYGQKPIKGS
Sbjct: 31  NGMKALISSHSPKRSRRSVEKRVVNVPMKEMEGSRHKGDTTPPSDSWAWRKYGQKPIKGS 90

Query: 221 PYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSR 280
           PYPRGYYRCSS+KGC ARKQVERSR +P M++ITYTSEHNHPWP     L  STR+ P  
Sbjct: 91  PYPRGYYRCSSTKGCPARKQVERSRDDPTMILITYTSEHNHPWP-----LTSSTRNGPK- 150

Query: 281 TTTATTANKTSPKHERINDILPTTTIKEEEMDED 315
                   K  PK E      P   ++ E  +ED
Sbjct: 151 -------PKPEPKPE------PEPEVEPEAEEED 165

BLAST of CSPI01G25350 vs. NCBI nr
Match: gi|778662047|ref|XP_011659293.1| (PREDICTED: probable WRKY transcription factor 14 [Cucumis sativus])

HSP 1 Score: 830.1 bits (2143), Expect = 1.7e-237
Identity = 413/413 (100.00%), Postives = 413/413 (100.00%), Query Frame = 1

Query: 1   MFCSLFEMDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSM 60
           MFCSLFEMDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSM
Sbjct: 1   MFCSLFEMDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSM 60

Query: 61  NFGDPLSFPTRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSSGDGVIST 120
           NFGDPLSFPTRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSSGDGVIST
Sbjct: 61  NFGDPLSFPTRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSSGDGVIST 120

Query: 121 PPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNSANKRRKSQV 180
           PPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNSANKRRKSQV
Sbjct: 121 PPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNSANKRRKSQV 180

Query: 181 KKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQ 240
           KKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQ
Sbjct: 181 KKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQ 240

Query: 241 VERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKHERINDI 300
           VERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKHERINDI
Sbjct: 241 VERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKHERINDI 300

Query: 301 LPTTTIKEEEMDEDQMTDKATTTNDNQEEGDHQDFPYDLIFTEFADQMNDLEHDNNHDHN 360
           LPTTTIKEEEMDEDQMTDKATTTNDNQEEGDHQDFPYDLIFTEFADQMNDLEHDNNHDHN
Sbjct: 301 LPTTTIKEEEMDEDQMTDKATTTNDNQEEGDHQDFPYDLIFTEFADQMNDLEHDNNHDHN 360

Query: 361 HHLSADPLNNNMLMFSNHGFSAGNGEGSKDPFLELYDWAENSSGSLFKEAKGG 414
           HHLSADPLNNNMLMFSNHGFSAGNGEGSKDPFLELYDWAENSSGSLFKEAKGG
Sbjct: 361 HHLSADPLNNNMLMFSNHGFSAGNGEGSKDPFLELYDWAENSSGSLFKEAKGG 413

BLAST of CSPI01G25350 vs. NCBI nr
Match: gi|659131380|ref|XP_008465657.1| (PREDICTED: probable WRKY transcription factor 14 isoform X1 [Cucumis melo])

HSP 1 Score: 768.5 bits (1983), Expect = 6.1e-219
Identity = 386/414 (93.24%), Postives = 398/414 (96.14%), Query Frame = 1

Query: 1   MFCSLFEMDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSM 60
           MFCSLFEMDNNNYQQAGDLTDVIR +SAPVAGHFSSEFSLD FSG+ +LWSHLPADNSSM
Sbjct: 1   MFCSLFEMDNNNYQQAGDLTDVIRPTSAPVAGHFSSEFSLDSFSGEGRLWSHLPADNSSM 60

Query: 61  NFGDPLSFPTRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSSGDGVIST 120
           NFGDPLSF TRDPFLLPHFSPFNFASDGGGEGLAAD QPSNLLSHMLQISPSSGDGVIST
Sbjct: 61  NFGDPLSFQTRDPFLLPHFSPFNFASDGGGEGLAAD-QPSNLLSHMLQISPSSGDGVIST 120

Query: 121 PPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNSANKRRKSQV 180
           PPCESLA+AVGNSP STSVRGGGG+V+PTGGSN LCLMENSGIQISSPRNSANKRRKSQV
Sbjct: 121 PPCESLASAVGNSPSSTSVRGGGGVVVPTGGSNPLCLMENSGIQISSPRNSANKRRKSQV 180

Query: 181 KKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQ 240
           KKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQ
Sbjct: 181 KKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQ 240

Query: 241 VERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKHERINDI 300
           VERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTT T  NKTSPK ERINDI
Sbjct: 241 VERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTTTATNKTSPKQERINDI 300

Query: 301 LPTTTIKEEEMDEDQMTDK-ATTTNDNQEEGDHQDFPYDLIFTEFADQMNDLEHDNNHDH 360
           LPTT IKEEE+DEDQ TDK ATTTNDNQEEGDHQDFP+DLIFTEFADQMNDLEH+N+HDH
Sbjct: 301 LPTTMIKEEEIDEDQTTDKAATTTNDNQEEGDHQDFPFDLIFTEFADQMNDLEHENDHDH 360

Query: 361 NHHLSADPLNNNMLMFSNHGFSAGNGEGSKDPFLELYDWAENSSGSLFKEAKGG 414
           +HHLSADPLNNNMLMF +HGFS GNGEGSKDPFLELYDWAENSSGSLFKEAKGG
Sbjct: 361 SHHLSADPLNNNMLMFGSHGFSTGNGEGSKDPFLELYDWAENSSGSLFKEAKGG 413

BLAST of CSPI01G25350 vs. NCBI nr
Match: gi|659131382|ref|XP_008465658.1| (PREDICTED: probable WRKY transcription factor 14 isoform X2 [Cucumis melo])

HSP 1 Score: 753.4 bits (1944), Expect = 2.0e-214
Identity = 382/414 (92.27%), Postives = 394/414 (95.17%), Query Frame = 1

Query: 1   MFCSLFEMDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSM 60
           MFCSLFEMDNNNYQQAGDLTDVIR +SAPVAGHFSSEFSLD FSG+ +LWSHLPADNSSM
Sbjct: 1   MFCSLFEMDNNNYQQAGDLTDVIRPTSAPVAGHFSSEFSLDSFSGEGRLWSHLPADNSSM 60

Query: 61  NFGDPLSFPTRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSSGDGVIST 120
           NFGDPLSF TRDPFLLPHFSPFNFASDGGGEGLAAD QPSNLLSHMLQISPSSGDGVIST
Sbjct: 61  NFGDPLSFQTRDPFLLPHFSPFNFASDGGGEGLAAD-QPSNLLSHMLQISPSSGDGVIST 120

Query: 121 PPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNSANKRRKSQV 180
           PPCESLA+AVGNSP STSVRGGGG+V+PTGGSN LCLMENSGIQISSPRNSANKRRKSQV
Sbjct: 121 PPCESLASAVGNSPSSTSVRGGGGVVVPTGGSNPLCLMENSGIQISSPRNSANKRRKSQV 180

Query: 181 KKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQ 240
           KKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYP    RCSSSKGCSARKQ
Sbjct: 181 KKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYP----RCSSSKGCSARKQ 240

Query: 241 VERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKHERINDI 300
           VERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTT T  NKTSPK ERINDI
Sbjct: 241 VERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTTTATNKTSPKQERINDI 300

Query: 301 LPTTTIKEEEMDEDQMTDK-ATTTNDNQEEGDHQDFPYDLIFTEFADQMNDLEHDNNHDH 360
           LPTT IKEEE+DEDQ TDK ATTTNDNQEEGDHQDFP+DLIFTEFADQMNDLEH+N+HDH
Sbjct: 301 LPTTMIKEEEIDEDQTTDKAATTTNDNQEEGDHQDFPFDLIFTEFADQMNDLEHENDHDH 360

Query: 361 NHHLSADPLNNNMLMFSNHGFSAGNGEGSKDPFLELYDWAENSSGSLFKEAKGG 414
           +HHLSADPLNNNMLMF +HGFS GNGEGSKDPFLELYDWAENSSGSLFKEAKGG
Sbjct: 361 SHHLSADPLNNNMLMFGSHGFSTGNGEGSKDPFLELYDWAENSSGSLFKEAKGG 409

BLAST of CSPI01G25350 vs. NCBI nr
Match: gi|698484442|ref|XP_009789029.1| (PREDICTED: probable WRKY transcription factor 14 [Nicotiana sylvestris])

HSP 1 Score: 269.6 bits (688), Expect = 8.9e-69
Identity = 166/321 (51.71%), Postives = 207/321 (64.49%), Query Frame = 1

Query: 101 NLLSHMLQISPSSGDGVISTPPCESLATAVGNSPRSTSVRGGGGLV----LPTGGSNSLC 160
           N+ S MLQISP++      +P    +  AV   P ST + GGG LV    + +  S+  C
Sbjct: 142 NIFSRMLQISPTTKLAAAMSPCNSPMTGAVALQPPSTVIAGGGALVANDAIISPNSSKTC 201

Query: 161 LMENSGIQISSPRNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKP 220
           LMENSG+QISSPRN+  KRRKSQ KKVVCIPAPAPANSR   GE+VPSDLWAWRKYGQKP
Sbjct: 202 LMENSGLQISSPRNTGIKRRKSQAKKVVCIPAPAPANSRQG-GEIVPSDLWAWRKYGQKP 261

Query: 221 IKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRS 280
           IKGSPYPRGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWPTQRN+LAGSTRS
Sbjct: 262 IKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNSLAGSTRS 321

Query: 281 HPS---RTTTATTANKTSPKHERINDILPTTTIKEEEMDEDQMTDKATTTNDNQEEGDHQ 340
            P+    TTT+T+ N T+ K ++ N+I     +   ++ E+          D +++  HQ
Sbjct: 322 QPNNSKHTTTSTSKNNTNSKDDQQNNISTVAQVNISKVKEE-----VAELQDLRDDHHHQ 381

Query: 341 DFPYDLIFTEFA-DQMNDLEHDNNHDHNH--------HLSADPLNNNMLMFSNHGFSAGN 400
               ++   EF+ D    +  D++ +H+H         L ADPLN   L+F+       N
Sbjct: 382 QLAVEMRDVEFSKDSYQPILPDSSSNHSHEDFFADLVELEADPLN---LLFAKTLSGDIN 441

Query: 401 GEGSKDPF--LELYDWAENSS 404
             G K       LYDW+ N++
Sbjct: 442 EAGQKKTIDAFNLYDWSGNNN 453

BLAST of CSPI01G25350 vs. NCBI nr
Match: gi|297740908|emb|CBI31090.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 269.2 bits (687), Expect = 1.2e-68
Identity = 196/440 (44.55%), Postives = 253/440 (57.50%), Query Frame = 1

Query: 3   CSLFEMDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSMNF 62
           CS F     NYQ  GDL D++R S +  A       +   F  +  ++S +  D    +F
Sbjct: 2   CSYFRQRMENYQ--GDLADIVRASGSGGARTSDEPVADWQFPSEPMIFSPV-VDEPLDDF 61

Query: 63  GDPLSFPTRDPFLLP-------HFSPFNFASDGGGEGLAADD--QPSNLLSHMLQISPSS 122
           GDP S   RDP L          F   N +    G+ +  +D  +P N+ S MLQISP++
Sbjct: 62  GDPFS-NMRDPLLHELGIAGSGFFGSSNSSGGMFGQKILDEDMKRPCNIFSRMLQISPNA 121

Query: 123 GDGVISTPPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNSAN 182
               +   PCES   A   +   TS      ++  +G S+  CLM+++ +QISSPRN   
Sbjct: 122 R---MPISPCESQIVAASPTGIKTSALAPNDMI--SGNSSKGCLMDSTALQISSPRNPGI 181

Query: 183 KRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 242
           KRRKSQ KKVVCIPAPA ANSR  SGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK
Sbjct: 182 KRRKSQAKKVVCIPAPAAANSRP-SGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 241

Query: 243 GCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPK 302
           GCSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTRS PS+   A+  + +   
Sbjct: 242 GCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSQPSKNNGASKNSGSCQS 301

Query: 303 HERINDILPTTTIKEEEMD---EDQMT----DKATTTNDNQEEGDHQDFPYDLIFTEFAD 362
            ++       T +KEE  +    D M+      +TT    +EE  + +   ++  +EF++
Sbjct: 302 TQK------ATGLKEENKESYNNDDMSPIVGGSSTTGASVKEEMGNVEKQLEMDDSEFSE 361

Query: 363 ----QMNDLEHDNNHDHNH-----HLSADPLNNNMLMFSNHGFSAGNGEGSK----DPFL 414
                      D+N   +       + ADPLN   L+F+  GFS+G+ E  +    DPF 
Sbjct: 362 GFPRSYKPAMPDSNQSEDFFADLGEIEADPLN---LLFA-QGFSSGDHEERESKALDPF- 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
WRK14_ARATH1.1e-5542.47Probable WRKY transcription factor 14 OS=Arabidopsis thaliana GN=WRKY14 PE=2 SV=... [more]
WRK35_ARATH1.1e-5341.23Probable WRKY transcription factor 35 OS=Arabidopsis thaliana GN=WRKY35 PE=2 SV=... [more]
WRK22_ARATH1.5e-3145.64WRKY transcription factor 22 OS=Arabidopsis thaliana GN=WRKY22 PE=2 SV=1[more]
WRK16_ARATH4.3e-3151.61Probable WRKY transcription factor 16 OS=Arabidopsis thaliana GN=WRKY16 PE=2 SV=... [more]
WRK65_ARATH4.8e-3050.00Probable WRKY transcription factor 65 OS=Arabidopsis thaliana GN=WRKY65 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0LYP6_CUCSA1.2e-237100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G547510 PE=4 SV=1[more]
A0A059A3C8_EUCGR1.0e-6342.80Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_K01971 PE=4 SV=1[more]
M5WIC8_PRUPE3.9e-6344.71Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004535mg PE=4 SV=1[more]
V4U7V4_9ROSI3.9e-6341.99Uncharacterized protein OS=Citrus clementina GN=CICLE_v10014984mg PE=4 SV=1[more]
A0A0D2UKC0_GOSRA2.5e-6248.82Uncharacterized protein OS=Gossypium raimondii GN=B456_011G006600 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G30650.16.4e-5742.47 WRKY DNA-binding protein 14[more]
AT2G34830.16.0e-5541.23 WRKY DNA-binding protein 35[more]
AT4G01250.18.4e-3345.64 WRKY family transcription factor[more]
AT5G45050.12.4e-3251.61 Disease resistance protein (TIR-NBS-LRR class)[more]
AT1G29280.12.7e-3150.00 WRKY DNA-binding protein 65[more]
Match NameE-valueIdentityDescription
gi|778662047|ref|XP_011659293.1|1.7e-237100.00PREDICTED: probable WRKY transcription factor 14 [Cucumis sativus][more]
gi|659131380|ref|XP_008465657.1|6.1e-21993.24PREDICTED: probable WRKY transcription factor 14 isoform X1 [Cucumis melo][more]
gi|659131382|ref|XP_008465658.1|2.0e-21492.27PREDICTED: probable WRKY transcription factor 14 isoform X2 [Cucumis melo][more]
gi|698484442|ref|XP_009789029.1|8.9e-6951.71PREDICTED: probable WRKY transcription factor 14 [Nicotiana sylvestris][more]
gi|297740908|emb|CBI31090.3|1.2e-6844.55unnamed protein product [Vitis vinifera][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR003657WRKY_dom
Vocabulary: Molecular Function
TermDefinition
GO:0003700transcription factor activity, sequence-specific DNA binding
GO:0043565sequence-specific DNA binding
Vocabulary: Biological Process
TermDefinition
GO:0006355regulation of transcription, DNA-templated
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005667 transcription factor complex
cellular_component GO:0005634 nucleus
molecular_function GO:0043565 sequence-specific DNA binding
molecular_function GO:0003700 transcription factor activity, sequence-specific DNA binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G25350.1CSPI01G25350.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainGENE3DG3DSA:2.20.25.80coord: 195..264
score: 1.1
IPR003657WRKY domainPFAMPF03106WRKYcoord: 205..262
score: 1.8
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 203..263
score: 6.0
IPR003657WRKY domainPROFILEPS50811WRKYcoord: 198..264
score: 32
IPR003657WRKY domainunknownSSF118290WRKY DNA-binding domaincoord: 201..264
score: 5.1
NoneNo IPR availablePANTHERPTHR32096FAMILY NOT NAMEDcoord: 152..372
score: 5.0
NoneNo IPR availablePANTHERPTHR32096:SF18DISEASE RESISTANCE PROTEIN-LIKE-RELATEDcoord: 152..372
score: 5.0