Cucsa.164290 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.164290
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionHeat Stress Transcription Factor family protein
Locationscaffold01148 : 149960 .. 152542 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAAAGTCAACGCCGGCGCCGTTTCTGATGAAGACTTACCGGATGGTGGAGGATCCGGCGACGGACGACGTGATATCGTGGAACAGCGACGGCACGGCGTTTGTGGTTTGGCAGACGGCGGAATTTGCTAAAGATGTTTTGCCAAAGCTCTTTAAGCATTCCAATTTCTCTAGCTTTGTTCGCCAACTTAATACCTATGTACGTTTTTCTTATTTACCTATTTCATAATACACTAAACAACTTTATTATTTTTTTTTTtAATTTTATGGTTCTTCAAATTCTCAAAATTTAAAGATTATGTAATGTAAAATTGGGCAACTTAATTTGGTTGTGTTAAATGGGTTTGTTCCGAGTTGAACTAATCAATTTGTAACATAGAAAaGAAAAAAAAaCACTATTGGACACAAAGAAAAAAaGAAGAGGAGTAATTACAATGGGTAGTTCTTTTAGACCTAAGAAGTATTAGGAAGAAAAAaGAAACCCCTTAGAAGTGAATCCCAAACTATACGTTTGTGTCTAATTGAATCTACTAAAATTAATTTATAGAAAGATAAAAGAATAGAAAAAAAAaCAAAAACTATTGAACTTTTCTATTTAGAGTTTGCTCGTTAACTTTGCATTCTAAAGTTCCATCAATTTCTCATGGTTGTATATAATTGCCTCATAAATCATCACCCATTTATTTGAATATTATATTACTTTCAAAAGGCTAATAAGAAAACTAAATAAATAATATCTTCTCCTTAAAATTTAATCTTATTTGGAAATGTAAGAAAGAAAACACCTTGTTGAAATACAAAACTCAACTCAACCTATTCCATTTTCTTATTCAATTTTtATTTCAATTTTTTTtACCCTGATAGATGTCTATTTGCTCTATAAGAATCTATTAAAAaTTTCCTTTATTTATAAATATTTTCAGTTGTTATCATTAATCTTTTTtGTAATTTCCAATTTTTtAATTAAAaCTTAACAAAACCTAAACCCCTCTTTCAAAATTGTATTTTATTTTATCTCAATCAGATAATTGTTTTACCCTTGTATTCTTTAAAACCAAAACAGGAAACAAAGTAAATATATTTATTGAAAAAAAAAaGGTTAATATATATTTTAACAAATTAATACAGGACTTTAATAATTATTAGTTTTAAAGTAAACTAATACTTCATTTGTCAGGTTCATAATAAATTTAGAATTATTTTCTTCTTTTTtATTTTGACTAAATTGATATTTCTGGTCCTACTTGAGACTTTAGGTGAGATTTTGACATCCACAACTTTAGTTTGCTAATTAAGTTTGATTTGCATTCATATTTCAGGCCCAAAAAATGATTTAAGTTGTACTTGTAGTTGTCTGACCTAACTTGATTTGAATTTGCATCATtAATTTTTTTTttttATTtGACATGTTTAATTAACTTTTtCACAAATAAATTAATGAATCATATTTGTTACCTATCTAAAAGAATGAAAACAAAATAAGAGAATTAATTTTTTCACAAATAAATTAATTTATCATATTTGTTACCCATCTAAAGGAATGAAAACAAAATAAGACAAACTTCAAAGCCTTAAAAAATCCCTATTAAGTATTAATTAAGCATTCTCATTTTTTTtGGGTTCCTTTTTTTtGTTATCGAAATTATTTTTCATTAAATATGGAAATTGTATATTCTTCCTTCTTTTATGCATCTTTGCAAAGTTCCATTATTTTAGACGTTCTTTTGTCATCAAAAGTTAGGTGATAGGAATTCTAATTATTATTTAATTTTCCAAAATATAATGTACATTTCAAACAATAACAATTTTTTTCACTTGTTTACTATGTAATATATACATATAAAACTTGTTTACTGCATAGTAATTCTTTTTTCTCACAAAAACATGTTTATTGTATAAATCTAATACCAGAAGTTAGCCTCATGTTAAAATCGGTTCAAACCCCCATAAAAATGATTTATGGATCCACCTTGGCTAGCAAACAAGGTTGGCTAAATAAAATCCACTAAGCTGGTCTCTTTATTTAGATTCTTGAGTTATCAGACTTGAGAAACATACTAAGCAACTAAGATTTCAAAACCATTTCTAGGAAGTTGAAAAGTAAACTAGGCATAAATCTTATTCATTCAAAATAGGGATTTATTTGTGTTTCTTTTTtGGAAAATAAAACTATAAATTGTTCAAGCCTTTTCTAATCAAGTTGCAAATTTTTTTATTCGTGTGTTTTTTTCTCTTGTTGAAGGGATTTCGGAAAGTGAGAACAACAAGGTGGGAGTTTTGCAATGAGAAATTTCAAAAGGGTGAAAAGGAAAAGCTTTGTGAAATACGTAGAAGAAAGAAATGGAGGAACAAGCGACAACACGAGGTAGATGAAGATCAAAGATCGACCTCATCAAACTCATCATCTTCACAGTACATTACTCTAATGGACGAGAACAAGAGGTTGAAGAAGGAAAATGGGGCCTTAAGCTCAGAACTTGCAAGCATGAAAAACAAATGCAAAGGGCTTTTTGATTTGGTGGCTACGTATGAAAATATTTCAAAGAAAGAAGATGAAGATGTGAGGCCAAAGCTGTTTGGA

mRNA sequence

ATGAGAAAGTCAACGCCGGCGCCGTTTCTGATGAAGACTTACCGGATGGTGGAGGATCCGGCGACGGACGACGTGATATCGTGGAACAGCGACGGCACGGCGTTTGTGGTTTGGCAGACGGCGGAATTTGCTAAAGATGTTTTGCCAAAGCTCTTTAAGCATTCCAATTTCTCTAGCTTTGTTCGCCAACTTAATACCTATGGATTTCGGAAAGTGAGAACAACAAGGTGGGAGTTTTGCAATGAGAAATTTCAAAAGGGTGAAAAGGAAAAGCTTTGTGAAATACGTAGAAGAAAGAAATGGAGGAACAAGCGACAACACGAGGTAGATGAAGATCAAAGATCGACCTCATCAAACTCATCATCTTCACAGTACATTACTCTAATGGACGAGAACAAGAGGTTGAAGAAGGAAAATGGGGCCTTAAGCTCAGAACTTGCAAGCATGAAAAACAAATGCAAAGGGCTTTTTGATTTGGTGGCTACGTATGAAAATATTTCAAAGAAAGAAGATGAAGATGTGAGGCCAAAGCTGTTTGGA

Coding sequence (CDS)

ATGAGAAAGTCAACGCCGGCGCCGTTTCTGATGAAGACTTACCGGATGGTGGAGGATCCGGCGACGGACGACGTGATATCGTGGAACAGCGACGGCACGGCGTTTGTGGTTTGGCAGACGGCGGAATTTGCTAAAGATGTTTTGCCAAAGCTCTTTAAGCATTCCAATTTCTCTAGCTTTGTTCGCCAACTTAATACCTATGGATTTCGGAAAGTGAGAACAACAAGGTGGGAGTTTTGCAATGAGAAATTTCAAAAGGGTGAAAAGGAAAAGCTTTGTGAAATACGTAGAAGAAAGAAATGGAGGAACAAGCGACAACACGAGGTAGATGAAGATCAAAGATCGACCTCATCAAACTCATCATCTTCACAGTACATTACTCTAATGGACGAGAACAAGAGGTTGAAGAAGGAAAATGGGGCCTTAAGCTCAGAACTTGCAAGCATGAAAAACAAATGCAAAGGGCTTTTTGATTTGGTGGCTACGTATGAAAATATTTCAAAGAAAGAAGATGAAGATGTGAGGCCAAAGCTGTTTGGA

Protein sequence

MRKSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSFVRQLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRKKWRNKRQHEVDEDQRSTSSNSSSSQYITLMDENKRLKKENGALSSELASMKNKCKGLFDLVATYENISKKEDEDVRPKLFG
Homology
[Close]
BLAST of Cucsa.164290 vs. Swiss-Prot
Match: HSFB3_ARATH (Heat stress transcription factor B-3 OS=Arabidopsis thaliana GN=HSFB3 PE=2 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 1.3e-48
Identity = 110/204 (53.92%), Postives = 127/204 (62.25%), Query Frame = 1 

Query: 6   PAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSFVRQLN 65
           P PFL+KTY++VEDP TD VISWN  GT FVVWQ AEFA+D+LP LFKH NFSSFVRQLN
Sbjct: 38  PPPFLVKTYKVVEDPTTDGVISWNEYGTGFVVWQPAEFARDLLPTLFKHCNFSSFVRQLN 97

Query: 66  TYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRKK--W-RNKRQHEV------------- 125
           TYGFRKV T RWEF NE F+KG++E +  IRRRK   W  NK  H+V             
Sbjct: 98  TYGFRKVTTIRWEFSNEMFRKGQRELMSNIRRRKSQHWSHNKSNHQVVPTTTMVNQEGHQ 157

Query: 126 -------DEDQRSTSSNSSSSQYITLMDENKRLKKENGALSSELASMKNKCKGLFDLVAT 181
                   EDQ+S S+ SSS  Y  L+DENK LK EN  LS EL   K KCK L +LV  
Sbjct: 158 RIGIDHHHEDQQS-SATSSSFVYTALLDENKCLKNENELLSCELGKTKKKCKQLMELVER 217

[Close]
BLAST of Cucsa.164290 vs. Swiss-Prot
Match: HSF24_SOLPE (Heat shock factor protein HSF24 OS=Solanum peruvianum GN=HSF24 PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 3.6e-43
Identity = 93/192 (48.44%), Postives = 120/192 (62.50%), Query Frame = 1 

Query: 2   RKSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSFV 61
           +++ PAPFL+KTY++V+D ATDDVISWN  GT FVVW+TAEFAKD+LPK FKH+NFSSFV
Sbjct: 3   QRTAPAPFLLKTYQLVDDAATDDVISWNEIGTTFVVWKTAEFAKDLLPKYFKHNNFSSFV 62

Query: 62  RQLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRKKWRN------------KRQHEV 121
           RQLNTYGFRK+   +WEF NE F++G+KE L  IRRRK   +                + 
Sbjct: 63  RQLNTYGFRKIVPDKWEFANENFKRGQKELLTAIRRRKTVTSTPAGGKSVAAGASASPDN 122

Query: 122 DEDQRSTSSNSSS--------------SQYITLMDENKRLKKENGALSSELASMKNKCKG 168
             D   +SS SS               SQ+  L DEN++LKK+N  LSSEL   K +C  
Sbjct: 123 SGDDIGSSSTSSPDSKNPGSVDTPGKLSQFTDLSDENEKLKKDNQMLSSELVQAKKQCNE 182

[Close]
BLAST of Cucsa.164290 vs. Swiss-Prot
Match: HSFB1_ARATH (Heat stress transcription factor B-1 OS=Arabidopsis thaliana GN=HSFB1 PE=2 SV=2)

HSP 1 Score: 161.4 bits (407), Expect = 9.3e-39
Identity = 85/173 (49.13%), Postives = 112/173 (64.74%), Query Frame = 1 

Query: 2   RKSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSFV 61
           ++S PAPFL KTY++V+D +TDDV+SWN +GTAFVVW+TAEFAKD+LP+ FKH+NFSSF+
Sbjct: 8   QRSVPAPFLSKTYQLVDDHSTDDVVSWNEEGTAFVVWKTAEFAKDLLPQYFKHNNFSSFI 67

Query: 62  RQLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRKK---------------WRNKRQ 121
           RQLNTYGFRK    +WEF N+ F++G ++ L +IRRRK                  +   
Sbjct: 68  RQLNTYGFRKTVPDKWEFANDYFRRGGEDLLTDIRRRKSVIASTAGKCVVVGSPSESNSG 127

Query: 122 HEVDEDQRSTSSNSSS-------SQYITLMDENKRLKKENGALSSELASMKNK 153
              D    STSS  SS       +    L  EN++LK+EN  LSSELA+ K +
Sbjct: 128 GGDDHGSSSTSSPGSSKNPGSVENMVADLSGENEKLKRENNNLSSELAAAKKQ 180

[Close]
BLAST of Cucsa.164290 vs. Swiss-Prot
Match: HFB2B_ORYSJ (Heat stress transcription factor B-2b OS=Oryza sativa subsp. japonica GN=HSFB2B PE=2 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 2.1e-38
Identity = 85/202 (42.08%), Postives = 116/202 (57.43%), Query Frame = 1 

Query: 2   RKSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSFV 61
           +++ P PFL KTY++V+DPA DDVISWN DG+ FVVW+ AEFA+D+LPK FKH+NFSSFV
Sbjct: 42  QRTVPTPFLTKTYQLVDDPAVDDVISWNDDGSTFVVWRPAEFARDLLPKYFKHNNFSSFV 101

Query: 62  RQLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRK---------------------- 121
           RQLNTYGFRK+   RWEF N+ F++GE+  LCEI RRK                      
Sbjct: 102 RQLNTYGFRKIVPDRWEFANDCFRRGERRLLCEIHRRKVTPPAPAATTAAVAAAIPMALP 161

Query: 122 KWRNKRQHEVDEDQRSTSSNSSSSQYITLM------------------DENKRLKKENGA 164
               +    V   +    S+SSS +   ++                  DEN+RL++EN  
Sbjct: 162 VTTTRDGSPVLSGEEQVISSSSSPEPPLVLPQAPSGSGSGGVASGDVGDENERLRRENAQ 221

[Close]
BLAST of Cucsa.164290 vs. Swiss-Prot
Match: HFB2A_ARATH (Heat stress transcription factor B-2a OS=Arabidopsis thaliana GN=HSFB2A PE=2 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 6.0e-38
Identity = 84/194 (43.30%), Postives = 117/194 (60.31%), Query Frame = 1 

Query: 2   RKSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSFV 61
           ++S P PFL KT+ +VED + DDVISWN DG++F+VW   +FAKD+LPK FKH+NFSSFV
Sbjct: 17  QRSIPTPFLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPKHFKHNNFSSFV 76

Query: 62  RQLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRK-------------KWRNK---- 121
           RQLNTYGF+KV   RWEF N+ F++GEK  L EI+RRK             + RN+    
Sbjct: 77  RQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRKITTTHQTVVAPSSEQRNQTMVV 136

Query: 122 --RQHEVDEDQRSTSSNSSSSQY-------------ITLMDENKRLKKENGALSSELASM 164
                  D +     S+S SS Y             + L++EN++L+ +N  L+ EL  M
Sbjct: 137 SPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNGGLSVELLEENEKLRSQNIQLNRELTQM 196

[Close]
BLAST of Cucsa.164290 vs. TrEMBL
Match: A0A151TU31_CAJCA (Heat stress transcription factor B-3 OS=Cajanus cajan GN=KK1_009724 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 6.0e-69
Identity = 135/184 (73.37%), Postives = 152/184 (82.61%), Query Frame = 1 

Query: 2   RKSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSFV 61
           RK TP PFLMKTY +VEDPATDDV+SWN +GTAFVVWQ AEFA+D+LP LFKHSNFSSFV
Sbjct: 18  RKCTPPPFLMKTYMLVEDPATDDVVSWNREGTAFVVWQPAEFARDLLPTLFKHSNFSSFV 77

Query: 62  RQLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRKKWRNKRQ-----HEVDEDQRST 121
           RQLNTYGFRKV T+RWEF N+KF+KGE+E L EIRRRK W NK+Q      + DEDQRS 
Sbjct: 78  RQLNTYGFRKVATSRWEFFNDKFKKGERELLHEIRRRKAWTNKQQPNAPNQDSDEDQRS- 137

Query: 122 SSNSSSSQYITLMDENKRLKKENGALSSELASMKNKCKGLFDLVATYENISKKEDEDVRP 181
           SS SSSS Y TL+DENKRLKKENG LSSEL SMK KCK L DLVA Y + +K+++ED RP
Sbjct: 138 SSTSSSSGYTTLVDENKRLKKENGVLSSELTSMKRKCKELLDLVAKYSSHAKEKEEDERP 197

[Close]
BLAST of Cucsa.164290 vs. TrEMBL
Match: B9II85_POPTR (Heat Stress Transcription Factor family protein OS=Populus trichocarpa GN=POPTR_0016s05680g PE=3 SV=2)

HSP 1 Score: 265.8 bits (678), Expect = 3.9e-68
Identity = 132/189 (69.84%), Postives = 154/189 (81.48%), Query Frame = 1 

Query: 1   MRKSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSF 60
           +RKSTP PFL+KTY +VEDPATD+VISWN +GT FVVWQ AEFA+D+LP LFKHSNFSSF
Sbjct: 11  VRKSTPPPFLLKTYMLVEDPATDEVISWNEEGTGFVVWQPAEFARDLLPTLFKHSNFSSF 70

Query: 61  VRQLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRKKWRNKRQ---------HEVDE 120
           VRQLNTYGFRKV T+RWEFCN+ F+KGE+E LC+IRRRK W +K+Q          E +E
Sbjct: 71  VRQLNTYGFRKVATSRWEFCNDMFRKGERELLCQIRRRKAWSSKQQPIAPIQVTTQEFEE 130

Query: 121 DQRSTSSNSSSSQYITLMDENKRLKKENGALSSELASMKNKCKGLFDLVATYENISKKED 180
           DQRS SS SSSS+Y TL+DENKRLKKENG LS+EL SM  KCK L DLVA Y ++ +KE+
Sbjct: 131 DQRS-SSTSSSSEYTTLVDENKRLKKENGVLSTELTSMNRKCKELLDLVAKYAHL-EKEE 190

[Close]
BLAST of Cucsa.164290 vs. TrEMBL
Match: A0A0U3SRJ2_VITPS (Transcription factor HsfB3a OS=Vitis pseudoreticulata PE=2 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 2.5e-67
Identity = 132/195 (67.69%), Postives = 152/195 (77.95%), Query Frame = 1 

Query: 1   MRKSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSF 60
           +RKSTP PFL+KTY +VEDPATD VISWNSDGTAFVVWQ AEFA+D+LP LFKHSNFSSF
Sbjct: 15  VRKSTPPPFLLKTYMLVEDPATDGVISWNSDGTAFVVWQPAEFARDLLPTLFKHSNFSSF 74

Query: 61  VRQLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRKKWRNKRQ-------------- 120
           VRQLNTYGFRKV T+RWEFCN+ F++GE+E LCEIRRRK W  ++Q              
Sbjct: 75  VRQLNTYGFRKVATSRWEFCNDMFRRGERELLCEIRRRKAWTKQQQQQQQAVGGPADQNG 134

Query: 121 -HEVDEDQRSTSSNSSSSQYITLMDENKRLKKENGALSSELASMKNKCKGLFDLVATYEN 180
             E++ED RS SS SSSS Y +L+DENKRLKKENG L SEL SMKNKCK L DLVA +  
Sbjct: 135 VQELEEDLRS-SSTSSSSGYSSLVDENKRLKKENGVLCSELTSMKNKCKELLDLVAMHAG 194

[Close]
BLAST of Cucsa.164290 vs. TrEMBL
Match: M5WB78_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa014675mg PE=3 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 2.5e-67
Identity = 131/200 (65.50%), Postives = 155/200 (77.50%), Query Frame = 1 

Query: 1   MRKSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSF 60
           +RKS+P PFL+KTY +VEDP TDDVISWN DG+AFVVWQ AEFA+D+LP LFKHSNFSSF
Sbjct: 14  VRKSSPPPFLLKTYMLVEDPTTDDVISWNDDGSAFVVWQPAEFARDLLPTLFKHSNFSSF 73

Query: 61  VRQLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRKKWRNKR--------------- 120
           VRQLNTYGFRKV T+RWEFCN+KF+KGEK++LC+IRRRK W +K+               
Sbjct: 74  VRQLNTYGFRKVSTSRWEFCNDKFRKGEKDQLCDIRRRKAWASKQQPINNIALNQAAQAM 133

Query: 121 --QHEVDEDQRSTSSNSSSSQYITLMDENKRLKKENGALSSELASMKNKCKGLFDLVATY 180
             Q E DEDQRS SS SSSS Y +L+DENKRLK+ENG LSSEL SMK KCK L DLVA Y
Sbjct: 134 QNQDEFDEDQRSNSSTSSSSDYSSLVDENKRLKQENGVLSSELTSMKRKCKELLDLVAKY 193

[Close]
BLAST of Cucsa.164290 vs. TrEMBL
Match: F6HKV6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0007g08750 PE=3 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 3.3e-67
Identity = 132/198 (66.67%), Postives = 153/198 (77.27%), Query Frame = 1 

Query: 1   MRKSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSF 60
           +RKSTP PFL+KTY +VEDPATD VISWNSDGTAFVVWQ AEFA+D+LP LFKHSNFSSF
Sbjct: 14  VRKSTPPPFLLKTYMLVEDPATDGVISWNSDGTAFVVWQPAEFARDLLPTLFKHSNFSSF 73

Query: 61  VRQLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRKKWRNKRQ-------------- 120
           VRQLNTYGFRKV T+RWEFCN+ F++GE+E LCEIRRRK W  ++Q              
Sbjct: 74  VRQLNTYGFRKVATSRWEFCNDMFRRGERELLCEIRRRKAWTKQQQQQQQQQQAVGGPAD 133

Query: 121 ----HEVDEDQRSTSSNSSSSQYITLMDENKRLKKENGALSSELASMKNKCKGLFDLVAT 180
                E+++DQRS SS SSSS Y +L+DENKRLKKENG L SEL SMKNKCK L DLVA 
Sbjct: 134 QNGVQELEDDQRS-SSTSSSSGYSSLVDENKRLKKENGVLCSELTSMKNKCKELLDLVAM 193

[Close]
BLAST of Cucsa.164290 vs. TAIR10
Match: AT2G41690.1 (AT2G41690.1 heat shock transcription factor B3)

HSP 1 Score: 194.1 bits (492), Expect = 7.3e-50
Identity = 110/204 (53.92%), Postives = 127/204 (62.25%), Query Frame = 1 

Query: 6   PAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSFVRQLN 65
           P PFL+KTY++VEDP TD VISWN  GT FVVWQ AEFA+D+LP LFKH NFSSFVRQLN
Sbjct: 38  PPPFLVKTYKVVEDPTTDGVISWNEYGTGFVVWQPAEFARDLLPTLFKHCNFSSFVRQLN 97

Query: 66  TYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRKK--W-RNKRQHEV------------- 125
           TYGFRKV T RWEF NE F+KG++E +  IRRRK   W  NK  H+V             
Sbjct: 98  TYGFRKVTTIRWEFSNEMFRKGQRELMSNIRRRKSQHWSHNKSNHQVVPTTTMVNQEGHQ 157

Query: 126 -------DEDQRSTSSNSSSSQYITLMDENKRLKKENGALSSELASMKNKCKGLFDLVAT 181
                   EDQ+S S+ SSS  Y  L+DENK LK EN  LS EL   K KCK L +LV  
Sbjct: 158 RIGIDHHHEDQQS-SATSSSFVYTALLDENKCLKNENELLSCELGKTKKKCKQLMELVER 217

[Close]
BLAST of Cucsa.164290 vs. TAIR10
Match: AT4G36990.1 (AT4G36990.1 heat shock factor 4)

HSP 1 Score: 161.4 bits (407), Expect = 5.2e-40
Identity = 85/173 (49.13%), Postives = 112/173 (64.74%), Query Frame = 1 

Query: 2   RKSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSFV 61
           ++S PAPFL KTY++V+D +TDDV+SWN +GTAFVVW+TAEFAKD+LP+ FKH+NFSSF+
Sbjct: 8   QRSVPAPFLSKTYQLVDDHSTDDVVSWNEEGTAFVVWKTAEFAKDLLPQYFKHNNFSSFI 67

Query: 62  RQLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRKK---------------WRNKRQ 121
           RQLNTYGFRK    +WEF N+ F++G ++ L +IRRRK                  +   
Sbjct: 68  RQLNTYGFRKTVPDKWEFANDYFRRGGEDLLTDIRRRKSVIASTAGKCVVVGSPSESNSG 127

Query: 122 HEVDEDQRSTSSNSSS-------SQYITLMDENKRLKKENGALSSELASMKNK 153
              D    STSS  SS       +    L  EN++LK+EN  LSSELA+ K +
Sbjct: 128 GGDDHGSSSTSSPGSSKNPGSVENMVADLSGENEKLKRENNNLSSELAAAKKQ 180

[Close]
BLAST of Cucsa.164290 vs. TAIR10
Match: AT5G62020.1 (AT5G62020.1 heat shock transcription factor B2A)

HSP 1 Score: 158.7 bits (400), Expect = 3.4e-39
Identity = 84/194 (43.30%), Postives = 117/194 (60.31%), Query Frame = 1 

Query: 2   RKSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSFV 61
           ++S P PFL KT+ +VED + DDVISWN DG++F+VW   +FAKD+LPK FKH+NFSSFV
Sbjct: 17  QRSIPTPFLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPKHFKHNNFSSFV 76

Query: 62  RQLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRK-------------KWRNK---- 121
           RQLNTYGF+KV   RWEF N+ F++GEK  L EI+RRK             + RN+    
Sbjct: 77  RQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRKITTTHQTVVAPSSEQRNQTMVV 136

Query: 122 --RQHEVDEDQRSTSSNSSSSQY-------------ITLMDENKRLKKENGALSSELASM 164
                  D +     S+S SS Y             + L++EN++L+ +N  L+ EL  M
Sbjct: 137 SPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNGGLSVELLEENEKLRSQNIQLNRELTQM 196

[Close]
BLAST of Cucsa.164290 vs. TAIR10
Match: AT1G46264.1 (AT1G46264.1 heat shock transcription factor B4)

HSP 1 Score: 148.3 bits (373), Expect = 4.6e-36
Identity = 66/97 (68.04%), Postives = 78/97 (80.41%), Query Frame = 1 

Query: 3   KSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSFVR 62
           K+ PAPFL KTY++V+DPATD V+SW  D T FVVW+  EFA+D+LP  FKH+NFSSFVR
Sbjct: 29  KAVPAPFLTKTYQLVDDPATDHVVSWGDDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR 88

Query: 63  QLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRK 100
           QLNTYGFRK+   RWEF NE F++GEK  LCEI RRK
Sbjct: 89  QLNTYGFRKIVPDRWEFANEFFKRGEKHLLCEIHRRK 125


HSP 2 Score: 30.0 bits (66), Expect = 1.8e+00
Identity = 13/30 (43.33%), Postives = 21/30 (70.00%), Query Frame = 1 

Query: 121 SSSQYITLMDENKRLKKENGALSSELASMK 151
           +++Q   L ++N+RL++ N  L SELA MK
Sbjct: 189 TAAQVTALSEDNERLRRSNTVLMSELAHMK 218

[Close]
BLAST of Cucsa.164290 vs. TAIR10
Match: AT4G11660.1 (AT4G11660.1 winged-helix DNA-binding transcription factor family protein)

HSP 1 Score: 144.8 bits (364), Expect = 5.1e-35
Identity = 65/98 (66.33%), Postives = 81/98 (82.65%), Query Frame = 1 

Query: 2   RKSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSFV 61
           ++S P PFL KTY++VEDP  D++ISWN DGT F+VW+ AEFA+D+LPK FKH+NFSSFV
Sbjct: 53  QRSIPTPFLTKTYQLVEDPVYDELISWNEDGTTFIVWRPAEFARDLLPKYFKHNNFSSFV 112

Query: 62  RQLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRK 100
           RQLNTYGFRKV   RWEF N+ F++GEK  L +I+RRK
Sbjct: 113 RQLNTYGFRKVVPDRWEFSNDCFKRGEKILLRDIQRRK 150


HSP 2 Score: 32.7 bits (73), Expect = 2.8e-01
Identity = 16/51 (31.37%), Postives = 30/51 (58.82%), Query Frame = 1 

Query: 113 QRSTSSNSSSSQYITLMDENKRLKKENGALSSELASMKNKCKGLFDLVATY 164
           QR+TS  ++      L++EN+RL+K+N  L  E+  +K     ++ L+A +
Sbjct: 218 QRTTSCTTAPE----LVEENERLRKDNERLRKEMTKLKGLYANIYTLMANF 264

[Close]
BLAST of Cucsa.164290 vs. NCBI nr
Match: gi|449463360|ref|XP_004149402.1| (PREDICTED: heat stress transcription factor B-3 [Cucumis sativus])

HSP 1 Score: 369.4 bits (947), Expect = 3.6e-99
Identity = 180/180 (100.00%), Postives = 180/180 (100.00%), Query Frame = 1 

Query: 1   MRKSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSF 60
           MRKSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSF
Sbjct: 32  MRKSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSF 91

Query: 61  VRQLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRKKWRNKRQHEVDEDQRSTSSNS 120
           VRQLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRKKWRNKRQHEVDEDQRSTSSNS
Sbjct: 92  VRQLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRKKWRNKRQHEVDEDQRSTSSNS 151

Query: 121 SSSQYITLMDENKRLKKENGALSSELASMKNKCKGLFDLVATYENISKKEDEDVRPKLFG 180
           SSSQYITLMDENKRLKKENGALSSELASMKNKCKGLFDLVATYENISKKEDEDVRPKLFG
Sbjct: 152 SSSQYITLMDENKRLKKENGALSSELASMKNKCKGLFDLVATYENISKKEDEDVRPKLFG 211

[Close]
BLAST of Cucsa.164290 vs. NCBI nr
Match: gi|659121625|ref|XP_008460754.1| (PREDICTED: LOW QUALITY PROTEIN: heat stress transcription factor B-3 [Cucumis melo])

HSP 1 Score: 339.7 bits (870), Expect = 3.0e-90
Identity = 166/180 (92.22%), Postives = 171/180 (95.00%), Query Frame = 1 

Query: 1   MRKSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSF 60
           MRKST APFLMKTYRMVEDP TDDVISWNSDGTAFVVWQTAEFAKD+LPKLFKHSNFSSF
Sbjct: 32  MRKSTTAPFLMKTYRMVEDPGTDDVISWNSDGTAFVVWQTAEFAKDILPKLFKHSNFSSF 91

Query: 61  VRQLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRKKWRNKRQHEVDEDQRSTSSNS 120
           VRQLNTYGFRKV TTRWEFCNEKFQKGEK+KLCEI+RRKKW NK+QH VDED+RSTSSNS
Sbjct: 92  VRQLNTYGFRKVTTTRWEFCNEKFQKGEKKKLCEIQRRKKWSNKQQHGVDEDRRSTSSNS 151

Query: 121 SSSQYITLMDENKRLKKENGALSSELASMKNKCKGLFDLVATYENISKKEDEDVRPKLFG 180
           SSSQY TLMDENKRLKKENGALSSELASMKNKCK LFDLVATYENISKKEDED R KLFG
Sbjct: 152 SSSQYNTLMDENKRLKKENGALSSELASMKNKCKELFDLVATYENISKKEDEDERXKLFG 211

[Close]
BLAST of Cucsa.164290 vs. NCBI nr
Match: gi|1012359320|gb|KYP70504.1| (Heat stress transcription factor B-3 [Cajanus cajan])

HSP 1 Score: 268.5 bits (685), Expect = 8.6e-69
Identity = 135/184 (73.37%), Postives = 152/184 (82.61%), Query Frame = 1 

Query: 2   RKSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSFV 61
           RK TP PFLMKTY +VEDPATDDV+SWN +GTAFVVWQ AEFA+D+LP LFKHSNFSSFV
Sbjct: 18  RKCTPPPFLMKTYMLVEDPATDDVVSWNREGTAFVVWQPAEFARDLLPTLFKHSNFSSFV 77

Query: 62  RQLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRKKWRNKRQ-----HEVDEDQRST 121
           RQLNTYGFRKV T+RWEF N+KF+KGE+E L EIRRRK W NK+Q      + DEDQRS 
Sbjct: 78  RQLNTYGFRKVATSRWEFFNDKFKKGERELLHEIRRRKAWTNKQQPNAPNQDSDEDQRS- 137

Query: 122 SSNSSSSQYITLMDENKRLKKENGALSSELASMKNKCKGLFDLVATYENISKKEDEDVRP 181
           SS SSSS Y TL+DENKRLKKENG LSSEL SMK KCK L DLVA Y + +K+++ED RP
Sbjct: 138 SSTSSSSGYTTLVDENKRLKKENGVLSSELTSMKRKCKELLDLVAKYSSHAKEKEEDERP 197

[Close]
BLAST of Cucsa.164290 vs. NCBI nr
Match: gi|743869673|ref|XP_011033345.1| (PREDICTED: heat stress transcription factor B-3-like [Populus euphratica])

HSP 1 Score: 266.5 bits (680), Expect = 3.3e-68
Identity = 132/189 (69.84%), Postives = 155/189 (82.01%), Query Frame = 1 

Query: 1   MRKSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSF 60
           +RKSTP PFL+KTY +VEDPATD+VISWN +GT FVVWQ AEFA+D+LP LFKHSNFSSF
Sbjct: 11  VRKSTPPPFLLKTYMLVEDPATDEVISWNDEGTGFVVWQPAEFARDLLPTLFKHSNFSSF 70

Query: 61  VRQLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRKKWRNKRQ---------HEVDE 120
            RQLNTYGFRKV T+RWEFCN+ F+KGE+E LC+IRRRK W +K+Q          E++E
Sbjct: 71  FRQLNTYGFRKVATSRWEFCNDMFRKGERELLCQIRRRKAWSSKQQPIAPIQVTTQELEE 130

Query: 121 DQRSTSSNSSSSQYITLMDENKRLKKENGALSSELASMKNKCKGLFDLVATYENISKKED 180
           DQRS SS SSSS+Y TL+DENKRLKKENG LS+EL SMK KCK L DLVA Y ++ +KE+
Sbjct: 131 DQRS-SSTSSSSEYTTLVDENKRLKKENGVLSTELTSMKRKCKELLDLVAKYAHL-EKEE 190

[Close]
BLAST of Cucsa.164290 vs. NCBI nr
Match: gi|566208889|ref|XP_002323320.2| (Heat Stress Transcription Factor family protein [Populus trichocarpa])

HSP 1 Score: 265.8 bits (678), Expect = 5.6e-68
Identity = 132/189 (69.84%), Postives = 154/189 (81.48%), Query Frame = 1 

Query: 1   MRKSTPAPFLMKTYRMVEDPATDDVISWNSDGTAFVVWQTAEFAKDVLPKLFKHSNFSSF 60
           +RKSTP PFL+KTY +VEDPATD+VISWN +GT FVVWQ AEFA+D+LP LFKHSNFSSF
Sbjct: 11  VRKSTPPPFLLKTYMLVEDPATDEVISWNEEGTGFVVWQPAEFARDLLPTLFKHSNFSSF 70

Query: 61  VRQLNTYGFRKVRTTRWEFCNEKFQKGEKEKLCEIRRRKKWRNKRQ---------HEVDE 120
           VRQLNTYGFRKV T+RWEFCN+ F+KGE+E LC+IRRRK W +K+Q          E +E
Sbjct: 71  VRQLNTYGFRKVATSRWEFCNDMFRKGERELLCQIRRRKAWSSKQQPIAPIQVTTQEFEE 130

Query: 121 DQRSTSSNSSSSQYITLMDENKRLKKENGALSSELASMKNKCKGLFDLVATYENISKKED 180
           DQRS SS SSSS+Y TL+DENKRLKKENG LS+EL SM  KCK L DLVA Y ++ +KE+
Sbjct: 131 DQRS-SSTSSSSEYTTLVDENKRLKKENGVLSTELTSMNRKCKELLDLVAKYAHL-EKEE 190

The following BLAST results are available for this feature:
BLAST of Cucsa.164290 vs. Swiss-Prot
Match NameE-valueIdentityDescription
HSFB3_ARATH1.3e-4853.92Heat stress transcription factor B-3 OS=Arabidopsis thaliana GN=HSFB3 PE=2 SV=1[more]
HSF24_SOLPE3.6e-4348.44Heat shock factor protein HSF24 OS=Solanum peruvianum GN=HSF24 PE=2 SV=1[more]
HSFB1_ARATH9.3e-3949.13Heat stress transcription factor B-1 OS=Arabidopsis thaliana GN=HSFB1 PE=2 SV=2[more]
HFB2B_ORYSJ2.1e-3842.08Heat stress transcription factor B-2b OS=Oryza sativa subsp. japonica GN=HSFB2B ... [more]
HFB2A_ARATH6.0e-3843.30Heat stress transcription factor B-2a OS=Arabidopsis thaliana GN=HSFB2A PE=2 SV=... [more]
BLAST of Cucsa.164290 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A151TU31_CAJCA6.0e-6973.37Heat stress transcription factor B-3 OS=Cajanus cajan GN=KK1_009724 PE=3 SV=1[more]
B9II85_POPTR3.9e-6869.84Heat Stress Transcription Factor family protein OS=Populus trichocarpa GN=POPTR_... [more]
A0A0U3SRJ2_VITPS2.5e-6767.69Transcription factor HsfB3a OS=Vitis pseudoreticulata PE=2 SV=1[more]
M5WB78_PRUPE2.5e-6765.50Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa014675mg PE=3 S... [more]
F6HKV6_VITVI3.3e-6766.67Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0007g08750 PE=3 SV=... [more]
BLAST of Cucsa.164290 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G41690.17.3e-5053.92 heat shock transcription factor B3[more]
AT4G36990.15.2e-4049.13 heat shock factor 4[more]
AT5G62020.13.4e-3943.30 heat shock transcription factor B2A[more]
AT1G46264.14.6e-3668.04 heat shock transcription factor B4[more]
AT4G11660.15.1e-3566.33 winged-helix DNA-binding transcription factor family protein[more]
BLAST of Cucsa.164290 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449463360|ref|XP_004149402.1|3.6e-99100.00PREDICTED: heat stress transcription factor B-3 [Cucumis sativus][more]
gi|659121625|ref|XP_008460754.1|3.0e-9092.22PREDICTED: LOW QUALITY PROTEIN: heat stress transcription factor B-3 [Cucumis me... [more]
gi|1012359320|gb|KYP70504.1|8.6e-6973.37Heat stress transcription factor B-3 [Cajanus cajan][more]
gi|743869673|ref|XP_011033345.1|3.3e-6869.84PREDICTED: heat stress transcription factor B-3-like [Populus euphratica][more]
gi|566208889|ref|XP_002323320.2|5.6e-6869.84Heat Stress Transcription Factor family protein [Populus trichocarpa][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000232HSF_DNA-bd
IPR011991Winged helix-turn-helix DNA-binding domain
IPR027725HSF_fam
Vocabulary: Molecular Function
TermDefinition
GO:0003700transcription factor activity, sequence-specific DNA binding
GO:0043565sequence-specific DNA binding
Vocabulary: Biological Process
TermDefinition
GO:0006355regulation of transcription, DNA-templated
Vocabulary: Cellular Component
TermDefinition
GO:0005634nucleus
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0009408 response to heat
cellular_component GO:0005634 nucleus
cellular_component GO:0005667 transcription factor complex
molecular_function GO:0043565 sequence-specific DNA binding
molecular_function GO:0003700 transcription factor activity, sequence-specific DNA binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.164290.1Cucsa.164290.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 47..59
score: 1.2E-19coord: 60..72
score: 1.2E-19coord: 9..32
score: 1.2
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 9..98
score: 8.0
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 5..98
score: 3.2
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 48..72
scor
IPR011991Winged helix-turn-helix DNA-binding domainGENE3DG3DSA:1.10.10.10coord: 5..98
score: 8.5
IPR011991Winged helix-turn-helix DNA-binding domainunknownSSF46785"Winged helix" DNA-binding domaincoord: 5..98
score: 2.45
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 1..165
score: 8.6
NoneNo IPR availableunknownCoilCoilcoord: 132..152
scor
NoneNo IPR availablePANTHERPTHR10015:SF168HEAT STRESS TRANSCRIPTION FACTOR B-2Acoord: 1..165
score: 8.6

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.164290Cla002516Watermelon (97103) v1cgywmB301
Cucsa.164290Csa4G171490Cucumber (Chinese Long) v2cgycuB246
Cucsa.164290MELO3C023104Melon (DHL92) v3.5.1cgymeB287
Cucsa.164290ClCG04G003410Watermelon (Charleston Gray)cgywcgB286
Cucsa.164290CSPI04G10760Wild cucumber (PI 183967)cgycpiB253
Cucsa.164290MELO3C023104.2Melon (DHL92) v3.6.1cgymedB282
Cucsa.164290CsaV3_4G011850Cucumber (Chinese Long) v3cgycucB260
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.164290Cucurbita pepo (Zucchini)cgycpeB0451
Cucsa.164290Cucurbita pepo (Zucchini)cgycpeB0452
Cucsa.164290Silver-seed gourdcarcgyB0233
Cucsa.164290Silver-seed gourdcarcgyB0332