Cucsa.153640 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.153640 vs. Swiss-Prot
Match: LOX21_SOLTU (Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 3.3e-172
Identity = 302/521 (57.97%), Postives = 380/521 (72.94%), Query Frame = 1

Query: 204 EFSEIKQRDQYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTPER 263
           E  ++ QRD+++W  D+EFAR TLAGLNPYSI+LV   P  SKLD   YGP ES+ T E 
Sbjct: 384 ESPQLVQRDKFSWFRDVEFARQTLAGLNPYSIRLVTEWPLRSKLDPKVYGPPESEITKEL 443

Query: 264 VQELLGCCITVGKALADKRLFVVDYHDTLMPYVRRVRKIKGTTLYGSRTLFFLNSDGTLM 323
           +++ +G  +TV +A+  K+LF++DYHD L+PYV +V ++KG+ LYGSRT+FFL   GTL 
Sbjct: 444 IEKEIGNYMTVEQAVQQKKLFILDYHDLLLPYVNKVNELKGSMLYGSRTIFFLTPQGTLK 503

Query: 324 PLGIELTRPPMDEHLEQWKEVFTPGT-NSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAH 383
           PL IELTRPP+D+   QWKEV++P   N+T  WLW+ AKAHVLSHDS  HQLV HWLR H
Sbjct: 504 PLAIELTRPPVDDK-PQWKEVYSPNDWNATGAWLWKLAKAHVLSHDSGYHQLVSHWLRTH 563

Query: 384 CCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSAASYSV 443
           CC EPY IA+NRQLS MHPIYRLLHPHFRY M INA AR+ LINA G+IE++F    Y++
Sbjct: 564 CCTEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALAREALINANGVIESSFFPGKYAI 623

Query: 444 ELSSSAY-KEWRFDMQALPEDLIHRGM-VERKRDSEGRDVLELTIKDYPFANDGLLLWNA 503
           ELSS AY  EWRFD +ALP++LI RG+ VE   +  G   L+L I+DYPFANDGL+LW+ 
Sbjct: 624 ELSSIAYGAEWRFDQEALPQNLISRGLAVEDPNEPHG---LKLAIEDYPFANDGLVLWDI 683

Query: 504 LLEWVTEYVNHYYGDEENAVINDTELQAWWGEIQEKGHPDKRE--GWFALRTRDDLIKIA 563
           L +WVT YVNHYY  + N + +D ELQAWW EI+  GH DKR+   W  L+T +DLI I 
Sbjct: 684 LKQWVTNYVNHYY-PQTNLIESDKELQAWWSEIKNVGHGDKRDEPWWPELKTPNDLIGII 743

Query: 564 STIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDKFLKDFINQPEKKLNEL 623
           +TI WV SGHHA+VNF QY+Y GY PNRPT+AR+ M TE+ + +  + F+N+PE+ L   
Sbjct: 744 TTIVWVTSGHHAAVNFGQYSYAGYFPNRPTVARSKMPTEDPTAEEWEWFMNKPEEALLRC 803

Query: 624 FPSDAQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDPSISNAFEKFKANLTDLEKKID 683
           FPS  QA  V   + +LS HSPDEEYIG+ IEP WA DP I+ AFE F   L +LE  ID
Sbjct: 804 FPSQIQATKVMAILDVLSNHSPDEEYIGEKIEPYWAEDPVINAAFEVFSGKLKELEGIID 863

Query: 684 ELNQNKDLKNRYGAGIIPYEAMKPRSKPGITGSGVPYSVSI 720
             N +  L NR GAG++PYE +KP S+PG+TG GVPYS+SI
Sbjct: 864 ARNNDSKLSNRNGAGVMPYELLKPYSEPGVTGKGVPYSISI 899

HSP 2 Score: 154.5 bits (389), Expect = 4.5e-36
Identity = 89/180 (49.44%), Postives = 117/180 (65.00%), Query Frame = 1

Query: 31  YIGEIVVAEGFGEIGAVIVELQEGVNERFIDTISVEAEEPPISVTFSCKSWVQPKGLIDH 90
           Y  + V+ + FGE+GA+++E  E   E ++  I ++       V  +C SWV  K     
Sbjct: 137 YEADFVIPQDFGEVGAILIE-NEHHKEMYVKNIVIDGFVHG-KVEITCNSWVHSKFDNPD 196

Query: 91  RRIFFSSNKSYLLGKTPRGLVKLREEDLANLRGEKEDGSVDRNERKAFERIYDYDLYNDL 150
           +RIFF+ NKSYL  +TP G+ +LREE+L  LRG   DG     ERK FERIYDYD+YNDL
Sbjct: 197 KRIFFT-NKSYLPSQTPSGVSRLREEELVTLRG---DGI---GERKVFERIYDYDVYNDL 256

Query: 151 GDPDLSM-DLKRPVLGGSEEYPYPRRCRTGRPPTQNDPASEKRVEEWFYIPRDEEFSEIK 210
           G+ D +  D KRPVLGG +E PYPRRC+TGRP ++ DP SE R   + Y+PRDE FSE+K
Sbjct: 257 GEADSNNDDAKRPVLGG-KELPYPRRCKTGRPRSKKDPLSETR-STFVYVPRDEAFSEVK 305

BLAST of Cucsa.153640 vs. Swiss-Prot
Match: LOX2_ARATH (Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana GN=LOX2 PE=1 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 5.3e-170
Identity = 297/512 (58.01%), Postives = 367/512 (71.68%), Query Frame = 1

Query: 211 RDQYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTPERVQELLGC 270
           RD+++WL D EFAR TLAGLNPYSIQLV+  P +SKLD   YG   S  T E V+  +  
Sbjct: 389 RDRFSWLRDDEFARQTLAGLNPYSIQLVEEWPLISKLDPAVYGDPTSLITWEIVEREVKG 448

Query: 271 CITVGKALADKRLFVVDYHDTLMPYVRRVRKIKGTTLYGSRTLFFLNSDGTLMPLGIELT 330
            +TV +AL +KRLFV+DYHD L+PYV +VR++  TTLY SRTLFFL+ D TL P+ IELT
Sbjct: 449 NMTVDEALKNKRLFVLDYHDLLLPYVNKVRELNNTTLYASRTLFFLSDDSTLRPVAIELT 508

Query: 331 RPPMDEHLEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHCCMEPYAI 390
            PP + +  QWK+VFTPG ++T  WLW  AK H +SHD+  HQL+ HWLR H C EPY I
Sbjct: 509 CPP-NINKPQWKQVFTPGYDATSCWLWNLAKTHAISHDAGYHQLISHWLRTHACTEPYII 568

Query: 391 ATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSAASYSVELSSSAY- 450
           A NRQLS MHPIYRLLHPHFRY M INA AR++L+N GGIIE  F    Y++ELSS+ Y 
Sbjct: 569 AANRQLSAMHPIYRLLHPHFRYTMEINARARQSLVNGGGIIETCFWPGKYALELSSAVYG 628

Query: 451 KEWRFDMQALPEDLIHRGMVERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWVTEYV 510
           K WRFD + LP DLI RG+ E  + +E    + LTI DYPFANDGL+LW+A+ EWVT+YV
Sbjct: 629 KLWRFDQEGLPADLIKRGLAEEDKTAEHG--VRLTIPDYPFANDGLILWDAIKEWVTDYV 688

Query: 511 NHYYGDEENAVINDTELQAWWGEIQEKGHPDKREG--WFALRTRDDLIKIASTIAWVGSG 570
            HYY DEE  + +D ELQ WW E++  GH DK++   W  L+T+DDLI + +TIAWV SG
Sbjct: 689 KHYYPDEE-LITSDEELQGWWSEVRNIGHGDKKDEPWWPVLKTQDDLIGVVTTIAWVTSG 748

Query: 571 HHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDKFLKDFINQPEKKLNELFPSDAQAAI 630
           HHA+VNF QY YGGY PNRPT  R  M TE+ +D+ LK+F   PEK L + +PS  QA +
Sbjct: 749 HHAAVNFGQYGYGGYFPNRPTTTRIRMPTEDPTDEALKEFYESPEKVLLKTYPSQKQATL 808

Query: 631 VKQTMFLLSIHSPDEEYIGDAIEPAWALDPSISNAFEKFKANLTDLEKKIDELNQNKDLK 690
           V  T+ LLS HSPDEEYIG+  E +WA +P I+ AFE+FK  L  LE  IDE N N  LK
Sbjct: 809 VMVTLDLLSTHSPDEEYIGEQQEASWANEPVINAAFERFKGKLQYLEGVIDERNVNITLK 868

Query: 691 NRYGAGIIPYEAMKPRSKPGITGSGVPYSVSI 720
           NR GAG++ YE +KP S+ G+TG GVPYS+SI
Sbjct: 869 NRAGAGVVKYELLKPTSEHGVTGMGVPYSISI 896

HSP 2 Score: 160.2 bits (404), Expect = 8.3e-38
Identity = 87/181 (48.07%), Postives = 118/181 (65.19%), Query Frame = 1

Query: 29  KKYIGEIVVAEGFGEIGAVIVELQEGVNERFIDTISVEAEEPPISVTFSCKSWVQPKGLI 88
           +KY  E  + E FG +GA+ ++ Q     R +    VE + P  S+TF+C+SWV PK + 
Sbjct: 135 EKYECEFEMPEDFGPVGAIKIQNQY---HRQLFLKGVELKLPGGSITFTCESWVAPKSVD 194

Query: 89  DHRRIFFSSNKSYLLGKTPRGLVKLREEDLANLRGEKEDGSVDRNERKAFERIYDYDLYN 148
             +RIFFS +KSYL  +TP  L K R+E+L  L+G+  +   +  E   FERIYDYD+YN
Sbjct: 195 PTKRIFFS-DKSYLPSQTPEPLKKYRKEELETLQGKNRE---EVGEFTKFERIYDYDVYN 254

Query: 149 DLGDPDLSMDLKRPVLGGSEEYPYPRRCRTGRPPTQNDPASEKRVEEWFYIPRDEEFSEI 208
           D+GDPD   +L RPV+GG   +PYPRRC+TGR P + DP+SE+R    FY+PRDEEFS  
Sbjct: 255 DVGDPDNDPELARPVIGGLT-HPYPRRCKTGRKPCETDPSSEQRYGGEFYVPRDEEFSTA 307

Query: 209 K 210
           K
Sbjct: 315 K 307

BLAST of Cucsa.153640 vs. Swiss-Prot
Match: LOX23_HORVU (Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare GN=LOX2.3 PE=1 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 1.4e-162
Identity = 280/519 (53.95%), Postives = 367/519 (70.71%), Query Frame = 1

Query: 204 EFSEIKQRDQYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTPER 263
           E  E+ +RD+++W  D EFAR TLAGLNP  I+ +   P +SKLD   YGP ES  + E 
Sbjct: 384 EVPEMLERDRFSWFRDEEFARQTLAGLNPICIRRLTEFPIVSKLDPAVYGPAESALSKEI 443

Query: 264 VQELLGCCITVGKALADKRLFVVDYHDTLMPYVRRVRKIKGTTLYGSRTLFFLNSDGTLM 323
           +++++   +TV +A+  KRLF++DYHD  +PYV RVR++  TTLYGSRT+FFL+ +GTLM
Sbjct: 444 LEKMMNGRMTVEEAMEKKRLFLLDYHDVFLPYVHRVRELPDTTLYGSRTVFFLSDEGTLM 503

Query: 324 PLGIELTRPPMDEHLEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHC 383
           PL IELTRP       QWK  FT G+++T+ WLW+ AKAHVL+HD+  HQLV HWLR H 
Sbjct: 504 PLAIELTRPQSPTK-PQWKRAFTHGSDATESWLWKLAKAHVLTHDTGYHQLVSHWLRTHA 563

Query: 384 CMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSAASYSVE 443
           C+EPY IATNRQLS MHP+YRLLHPHFRY M INA AR+ LINA GIIE AF A  YS+E
Sbjct: 564 CVEPYIIATNRQLSRMHPVYRLLHPHFRYTMEINALAREALINADGIIEEAFLAGKYSIE 623

Query: 444 LSSSAY-KEWRFDMQALPEDLIHRGMVERKRDSEGRDVLELTIKDYPFANDGLLLWNALL 503
           LSS AY   W+F+ +ALPEDLI+RG+  R+ D E    LEL IKDYP+A+DGLL+W ++ 
Sbjct: 624 LSSVAYGAAWQFNTEALPEDLINRGLAVRRDDGE----LELAIKDYPYADDGLLIWGSIK 683

Query: 504 EWVTEYVNHYYGDEENAVINDTELQAWWGEIQEKGHPDKREG--WFALRTRDDLIKIAST 563
           +W ++YV+ YY  + + V  D EL+AWW E++ KGH DK++   W    T+++L++I + 
Sbjct: 684 QWASDYVDFYYKSDGD-VAGDEELRAWWEEVRTKGHADKKDEPWWPVCDTKENLVQILTI 743

Query: 564 IAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDKFLKDFINQPEKKLNELFP 623
           I WV SGHHA+VNF QY Y GY PNRPT+ R N+  E + D  +K F+ +PE+ L +  P
Sbjct: 744 IMWVTSGHHAAVNFGQYHYAGYFPNRPTVVRRNIPVEENRDDEMKKFMARPEEVLLQSLP 803

Query: 624 SDAQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDPSISNAFEKFKANLTDLEKKIDEL 683
           S  QA  V  T+ +LS HSPDEEY+G+  EPAW  +P +  AFEKF   L + E  ID  
Sbjct: 804 SQMQAIKVMATLDILSSHSPDEEYMGEYAEPAWLAEPMVKAAFEKFSGRLKEAEGTIDMR 863

Query: 684 NQNKDLKNRYGAGIIPYEAMKPRSKPGITGSGVPYSVSI 720
           N N + KNR GAGI+PYE +KP S+PG+TG G+P S+SI
Sbjct: 864 NNNPENKNRCGAGIVPYELLKPFSEPGVTGRGIPNSISI 896

HSP 2 Score: 147.9 bits (372), Expect = 4.3e-34
Identity = 86/182 (47.25%), Postives = 113/182 (62.09%), Query Frame = 1

Query: 31  YIGEIVVAEGFGEIGAVIVELQEGVNERFIDTIS-VEAEEPPISVTFSCKSWVQPKGLID 90
           Y  ++ V   FG +GAV+VE  E   E FI  I  +   +   ++TF   SWV  K    
Sbjct: 134 YEAKMSVPASFGPVGAVLVE-NEHHREMFIKDIKLITGGDESTAITFDVASWVHSKFDDP 193

Query: 91  HRRIFFSSNKSYLLGKTPRGLVKLREEDLANLRGEKEDGSVDRNERKAFERIYDYDLYND 150
             R FF+  KSYL  +TP G+  LR+++L  LRG+        +ERK  ER+YDYD YND
Sbjct: 194 EPRAFFTV-KSYLPSQTPPGIEALRKKELETLRGD------GHSERKFHERVYDYDTYND 253

Query: 151 LGDPDLSMDLKRPVLGGSEEYPYPRRCRTGRPPTQNDPASEKRVEEWFYIPRDEEFSEIK 210
           LGDPD ++D KRPVL G++E+PYPRRCRTGRP T  DP +E R     Y+PRDE+FS++K
Sbjct: 254 LGDPDKNIDHKRPVL-GTKEHPYPRRCRTGRPKTLYDPETETRSSP-VYVPRDEQFSDVK 305

Query: 211 QR 212
            R
Sbjct: 314 GR 305

BLAST of Cucsa.153640 vs. Swiss-Prot
Match: LOX21_HORVU (Lipoxygenase 2.1, chloroplastic OS=Hordeum vulgare GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 1.8e-157
Identity = 282/531 (53.11%), Postives = 362/531 (68.17%), Query Frame = 1

Query: 204 EFSEIKQRDQYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTPER 263
           E  EI  RD++ W  D EFAR T+AG+NP SIQLV   P  S LDE  YGP +S  T E 
Sbjct: 413 ETPEIHDRDKFAWFRDEEFARQTIAGMNPMSIQLVTEFPIKSNLDEATYGPADSLITKEV 472

Query: 264 VQELLGCCITVGKALADKRLFVVDYHDTLMPYVRRVRKIKGTTLYGSRTLFFLNSDGTLM 323
           V+E +   +T  +A+ +K+LF++DYHD L+PYV +VRK+ GTTLYGSR LFFL +DGTL 
Sbjct: 473 VEEQIRRVMTADEAVQNKKLFMLDYHDLLLPYVHKVRKLDGTTLYGSRALFFLTADGTLR 532

Query: 324 PLGIELTRPPMDEHLEQWKEVFTPGTNS--TDLWLWRFAKAHVLSHDSCIHQLVIHWLRA 383
           P+ IELTR P  +   QW++VFTPG +   T  WLW+ AKAH+L+HD+ +HQLV HWLR 
Sbjct: 533 PIAIELTR-PKSKKKPQWRQVFTPGCDGSVTGSWLWQLAKAHILAHDAGVHQLVSHWLRT 592

Query: 384 HCCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSAASYS 443
           H C EPY IA NRQLS MHP+YRLLHPHFR+ M INA AR  LINAGGIIE +F    YS
Sbjct: 593 HACTEPYIIAANRQLSQMHPVYRLLHPHFRFTMEINAQARAMLINAGGIIEGSFVPGEYS 652

Query: 444 VELSSSAY-KEWRFDMQALPEDLIHRGMVERKRDSEGRDVLELTIKDYPFANDGLLLWNA 503
           +ELSS AY ++WRFDM+ALPEDLI RGM  R  + E    LEL I+DYP+ANDGLL+W+A
Sbjct: 653 LELSSVAYDQQWRFDMEALPEDLIRRGMAVRNPNGE----LELAIEDYPYANDGLLVWDA 712

Query: 504 LLEWVTEYVNHYYGDEENAVINDTELQAWWGEIQEKGHPDKRE--GWFALRTRDDLIKIA 563
           + +W   YV HYY    + +++D ELQAWW E++ KGH DK++   W  L + ++L +  
Sbjct: 713 IKQWALTYVQHYYPCAAD-IVDDEELQAWWTEVRTKGHADKQDEPWWPELDSHENLAQTL 772

Query: 564 STIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDKFLKDFINQPEKKLNEL 623
           +TI WV SGHHA+VNF QY   GY+PNRPT+AR NM TE   D  ++DF+  PEK L + 
Sbjct: 773 ATIMWVTSGHHAAVNFGQYPMAGYIPNRPTMARRNMPTEIGGDD-MRDFVEAPEKVLLDT 832

Query: 624 FPSDAQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDPSISNAFEKFKANLTDLEKKID 683
           FPS  Q+AIV   + LLS HS DEEY+G   EPAW  D  I+ AFE+FK +   + +++D
Sbjct: 833 FPSQYQSAIVLAILDLLSTHSSDEEYMGTHEEPAWTKDGVINQAFEEFKESTRKIVEQVD 892

Query: 684 ELNQNKDLKNRYGAGIIPYEAMKPRSKPGITGS----------GVPYSVSI 720
           E N + D KNR+GAG++PY  ++P       G           G+P S+SI
Sbjct: 893 EWNNDPDRKNRHGAGMVPYVLLRPSDGDPTDGDPTDEKMVMEMGIPNSISI 936

HSP 2 Score: 136.7 bits (343), Expect = 9.8e-31
Identity = 82/185 (44.32%), Postives = 107/185 (57.84%), Query Frame = 1

Query: 27  EYKKYIGEIVVAEGFGEIGAVIVELQEGVNERFIDTISVEAEEPPISVTFSCKSWVQPKG 86
           E+  Y     V   FG IGAV V           D       +   +VTF CKSW+ P  
Sbjct: 148 EWDMYEATFKVPASFGPIGAVQVTNYHHSEMLLGDIEVFPTGQEESAVTFHCKSWIDPSH 207

Query: 87  LIDHRRIFFSSNKSYLLGKTPRGLVKLREEDLANLRGEKEDGSVDRNERKAFERIYDYDL 146
               +R+FF ++ SYL  +TP+G+  LR+ +L  LRG          ERK  +RIYDYD+
Sbjct: 208 CTPDKRVFFPAH-SYLPSQTPKGVEGLRKRELEILRG------TGCGERKEHDRIYDYDV 267

Query: 147 YNDLGDPDLSMD-LKRPVLGGSEEYPYPRRCRTGRPPTQNDPASEKRV-EEWFYIPRDEE 206
           YNDLG+PD   +   RPVLGG +E+PYPRRCRTGRP ++ DP SE+R  +E  Y+PRDE 
Sbjct: 268 YNDLGNPDDDNNPTTRPVLGG-KEHPYPRRCRTGRPRSKKDPFSEERSHKEHIYVPRDEA 324

Query: 207 FSEIK 210
           F+E K
Sbjct: 328 FTERK 324

BLAST of Cucsa.153640 vs. Swiss-Prot
Match: LOXC2_ORYSJ (Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX2 PE=2 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 1.0e-152
Identity = 271/514 (52.72%), Postives = 351/514 (68.29%), Query Frame = 1

Query: 210 QRDQYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTPERVQELLG 269
           Q+D++ WL D EFAR TLAG+NPY+I+LV+  P  SKLD   YGP ES  T + ++E + 
Sbjct: 432 QKDKFAWLRDEEFARETLAGINPYAIELVREFPLKSKLDPAVYGPAESAITADLLEEQMR 491

Query: 270 CCITVGKALADKRLFVVDYHDTLMPYVRRVRKIKGTTLYGSRTLFFLNSDGTLMPLGIEL 329
             +TV +A++ KRLF++D+HD  +PYV ++R +K TT+YGSRT+FFL  DGTL  L IEL
Sbjct: 492 RVMTVEEAISQKRLFMLDFHDLFLPYVHKIRSLKHTTMYGSRTIFFLTDDGTLRLLAIEL 551

Query: 330 TRPPMDEHLEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHCCMEPYA 389
           TRP       QW++VFTP T++T  WLWR AKAHV +HD+  H+L+ HWLR HC +EPY 
Sbjct: 552 TRPASPSQ-PQWRQVFTPSTDTTKSWLWRMAKAHVRAHDAGHHELITHWLRTHCAVEPYI 611

Query: 390 IATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSAASYSVELSSSAY 449
           IA NRQLS MHPIY+LLHPHFRY MRINA AR  LI+A GIIE +FS   YS+ELSS AY
Sbjct: 612 IAANRQLSEMHPIYQLLHPHFRYTMRINALARSRLISAAGIIELSFSPQKYSMELSSVAY 671

Query: 450 -KEWRFDMQALPEDLIHRGMVERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWVTEY 509
            K WRFDM+ALP DL+ RGM E   D      L L I+DYPFANDGLL+W+A+  WV  Y
Sbjct: 672 DKLWRFDMEALPADLVRRGMAE--EDPTAEHGLRLAIEDYPFANDGLLIWDAIKTWVQAY 731

Query: 510 VNHYYGDEENAVINDTELQAWWGEIQEKGHPDKREG--WFALRTRDDLIKIASTIAWVGS 569
           V  +Y D + +V  D ELQA+W E++ KGH DK++   W  L + + L    +TI WV +
Sbjct: 732 VARFYPDAD-SVAGDEELQAFWTEVRTKGHGDKKDAPWWPKLDSPESLAHTLTTIVWVAA 791

Query: 570 GHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSD-KFLKDFINQPEKKLNELFPSDAQA 629
            HHA+VNF QY +GGY PNRP+IART M  E   D   ++ F++ P++ L E FPS  QA
Sbjct: 792 AHHAAVNFGQYDFGGYFPNRPSIARTVMPVEEPVDGAAMERFLDNPDQALRECFPSQVQA 851

Query: 630 AIVKQTMFLLSIHSPDEEYIGDAIEPAWALDPSISNAFEKFKANLTDLEKKIDELNQNKD 689
            +V   + +LS HS DEEY+G      W  D ++  A+  F A L ++E  ID  N+++ 
Sbjct: 852 TVVMAVLDVLSTHSTDEEYLGGEQTRPWNSDAAVQAAYAGFTARLKEIEGVIDGRNKDRK 911

Query: 690 LKNRYGAGIIPYEAMKPRSKPGITGSGVPYSVSI 720
           LKNR GAGI+PY+ MKP S  G+TG G+P S SI
Sbjct: 912 LKNRCGAGILPYQLMKPFSDAGVTGMGIPNSTSI 941

HSP 2 Score: 166.8 bits (421), Expect = 8.9e-40
Identity = 93/186 (50.00%), Postives = 121/186 (65.05%), Query Frame = 1

Query: 31  YIGEIVVAEGFGEIGAVIVELQEGVNERFIDTISVEAEEPPISVT---FSCKSWVQPKGL 90
           Y  +  V  GFG IGAV+V  + G  E F++ +++ A +   + T     C SWVQPK  
Sbjct: 169 YEADFDVPTGFGPIGAVVVTNELG-QEMFLEDLNLTAGDGAGNSTVLPIRCNSWVQPKSS 228

Query: 91  IDH----RRIFFSSNKSYLLGKTPRGLVKLREEDLANLRGEKEDGSVDRNERKAFERIYD 150
           ID     +RIFF+  K+YL G+TP GL   REEDL   RG          +R+A +R+YD
Sbjct: 229 IDEGTPGKRIFFA--KAYLPGQTPAGLRSYREEDLKQKRGN------GAGQREADDRVYD 288

Query: 151 YDLYNDLGDPDLSMDLKRPVLGGSEEYPYPRRCRTGRPPTQNDPASEKRVEEWFYIPRDE 210
           YD+YNDLG+PD + DL RPVLGGS+++PYPRRCRTGRPP++ DP SE R     Y+PRDE
Sbjct: 289 YDVYNDLGNPDSNGDLARPVLGGSKQFPYPRRCRTGRPPSKKDPKSETRKGN-VYVPRDE 344

BLAST of Cucsa.153640 vs. TrEMBL
Match: K7LX80_SOYBN (Lipoxygenase OS=Glycine max GN=GLYMA_13G075900 PE=3 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 5.5e-206
Identity = 386/682 (56.60%), Postives = 475/682 (69.65%), Query Frame = 1

Query: 41  FGEIGAVIVELQEGVNERFIDTISVEAEEPPISVTFSCKSWVQPKGLIDHRRIFFSSNKS 100
           FG +GAV+V+  E   E F+ +I +  + P   V F+C SWVQPK     +R+FFS +KS
Sbjct: 161 FGNVGAVLVQ-HEHHKEMFLRSIVLH-DVPYGPVHFTCNSWVQPKHDCPVKRVFFS-DKS 220

Query: 101 YLLGKTPRGLVKLREEDLANLRGEKEDGSVDRNERKAFERIYDYDLYNDLGDPDLSMDLK 160
           YL  +TP GL +LRE +L  LRG  E       ERK++ERIYDYD+YNDLGDPD S+DLK
Sbjct: 221 YLPSQTPCGLRRLREVELMLLRGNGE------GERKSYERIYDYDVYNDLGDPDFSIDLK 280

Query: 161 RPVLGGSEEYPYPRRCRTGRPPTQNDPASEKRVEEWFYIPRDEEFSEIKQRDQYNWLSDI 220
           RP+LG SE +PYPRRCRTGR   ++  A                       D++ W SD 
Sbjct: 281 RPILGCSE-HPYPRRCRTGR---EHSIAG----------------------DRFFWFSDE 340

Query: 221 EFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTPERVQELLGCCITVGKALAD 280
           EFAR TL+G+NPYSIQLVK  P  SKLD   YGP ES  T E ++  +    TV +A+ +
Sbjct: 341 EFARETLSGVNPYSIQLVKEWPLRSKLDPEIYGPPESAITREIIESQIIGYRTVEEAIVE 400

Query: 281 KRLFVVDYHDTLMPYVRRVRKIKGTTLYGSRTLFFLNSDGTLMPLGIELTRPPMDEHLEQ 340
           K+LF++DYHD  +PYV +VR+IKGTTLYGSRTLFFL   GTL PL IELTRP MD    Q
Sbjct: 401 KKLFMLDYHDLFLPYVSKVREIKGTTLYGSRTLFFLTKQGTLKPLAIELTRPIMDGK-PQ 460

Query: 341 WKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTMH 400
           WK+VFTP ++STDLWLWR AKAHVL+HDS  H+LV HWLR HC +EP+ IATNRQLSTMH
Sbjct: 461 WKQVFTPASHSTDLWLWRLAKAHVLAHDSGYHELVNHWLRTHCALEPFIIATNRQLSTMH 520

Query: 401 PIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSAASYSVELSSSAYKE-WRFDMQAL 460
           P+Y+LLHPH RY M IN+ AR+ LI A GIIE +FS   YS+E+SS AY + WRFD+QAL
Sbjct: 521 PVYKLLHPHMRYTMEINSLAREVLICANGIIEISFSTNKYSMEISSVAYDQLWRFDLQAL 580

Query: 461 PEDLIHRGMVERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWVTEYVNHYYGDEENA 520
           P DLIHRGM     D      L LTI+DYPFANDGLL+W+A+ +WVTEY+NHYY +  + 
Sbjct: 581 PNDLIHRGMA--LEDPNAPQGLMLTIEDYPFANDGLLIWDAIKQWVTEYINHYYSNS-SV 640

Query: 521 VINDTELQAWWGEIQEKGHPDK-REGWF-ALRTRDDLIKIASTIAWVGSGHHASVNFLQY 580
           V +D ELQAWW EIQ+ GH DK +E W+ +L T  DLI I +TIAW+ SGHHA+VNF QY
Sbjct: 641 VKSDQELQAWWTEIQKVGHGDKYKEPWWPSLETPKDLIDIITTIAWIASGHHAAVNFAQY 700

Query: 581 AYGGYMPNRPTIARTNMLTENHSDKFLKDFINQPEKKLNELFPSDAQAAIVKQTMFLLSI 640
            YGGY PNRPTIAR  M TE+ S +  ++F+  PE+ L E  PS  QA +V   + LLS 
Sbjct: 701 TYGGYFPNRPTIARIKMPTEDPSKEEWENFLKNPEQTLLECLPSQIQATLVMVILNLLSN 760

Query: 641 HSPDEEYIGDAIEPAWALDPSISNAFEKFKANLTDLEKKIDELNQNKDLKNRYGAGIIPY 700
           HSPDEEYIG  +EP+WA + +I  +FE+F   L ++E  ID  N N +LKNR GAG++PY
Sbjct: 761 HSPDEEYIGQYMEPSWAENQTIKTSFERFNKRLKEIEGIIDSRNGNYNLKNRCGAGLVPY 803

Query: 701 EAMKPRSKPGITGSGVPYSVSI 720
           E MKP S PGITG GVPYS SI
Sbjct: 821 ELMKPFSGPGITGKGVPYSASI 803

BLAST of Cucsa.153640 vs. TrEMBL
Match: K7LX81_SOYBN (Lipoxygenase OS=Glycine max PE=3 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 2.8e-202
Identity = 379/680 (55.74%), Postives = 468/680 (68.82%), Query Frame = 1

Query: 74  VTFSCKSWVQPKGLIDHRRIFFSSNKSYLLGKTPRGLVKLREEDLANLRGEKEDGSVDRN 133
           V F+C SWVQPK     +R+FF S+KSYL  +TP GL +LRE +L  LRG  E       
Sbjct: 16  VHFTCNSWVQPKHDCPVKRVFF-SDKSYLPSQTPCGLRRLREVELMLLRGNGE------G 75

Query: 134 ERKAFERIYDYDLYNDLGDPDLSMDLKRPVLGGSEEYPYPRRCRTGRPPTQNDPASEKRV 193
           ERK++ERIYDYD+YNDLGDPD S+DLKRP+LG S E+PYPRRCRTGR  +    AS + +
Sbjct: 76  ERKSYERIYDYDVYNDLGDPDFSIDLKRPILGCS-EHPYPRRCRTGREHSIAGFASFQDI 135

Query: 194 E----EWFYIPRDE------------EFSEIKQRDQYN---------------WLSDIEF 253
           +    E +++P  E             F ++   ++                 W SD EF
Sbjct: 136 DMLYKEGYHLPHPEPKWLTLLQKVIPSFIKVATDNKKTLHFDTPEAVKRDRFFWFSDEEF 195

Query: 254 ARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTPERVQELLGCCITVGKALADKR 313
           AR TL+G+NPYSIQLVK  P  SKLD   YGP ES  T E ++  +    TV +A+ +K+
Sbjct: 196 ARETLSGVNPYSIQLVKEWPLRSKLDPEIYGPPESAITREIIESQIIGYRTVEEAIVEKK 255

Query: 314 LFVVDYHDTLMPYVRRVRKIKGTTLYGSRTLFFLNSDGTLMPLGIELTRPPMDEHLEQWK 373
           LF++DYHD  +PYV +VR+IKGTTLYGSRTLFFL   GTL PL IELTRP MD    QWK
Sbjct: 256 LFMLDYHDLFLPYVSKVREIKGTTLYGSRTLFFLTKQGTLKPLAIELTRPIMDGK-PQWK 315

Query: 374 EVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTMHPI 433
           +VFTP ++STDLWLWR AKAHVL+HDS  H+LV HWLR HC +EP+ IATNRQLSTMHP+
Sbjct: 316 QVFTPASHSTDLWLWRLAKAHVLAHDSGYHELVNHWLRTHCALEPFIIATNRQLSTMHPV 375

Query: 434 YRLLHPHFRYNMRINANARKNLINAGGIIENAFSAASYSVELSSSAYKE-WRFDMQALPE 493
           Y+LLHPH RY M IN+ AR+ LI A GIIE +FS   YS+E+SS AY + WRFD+QALP 
Sbjct: 376 YKLLHPHMRYTMEINSLAREVLICANGIIEISFSTNKYSMEISSVAYDQLWRFDLQALPN 435

Query: 494 DLIHRGMVERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWVTEYVNHYYGDEENAVI 553
           DLIHRGM     D      L LTI+DYPFANDGLL+W+A+ +WVTEY+NHYY +  + V 
Sbjct: 436 DLIHRGMA--LEDPNAPQGLMLTIEDYPFANDGLLIWDAIKQWVTEYINHYYSN-SSVVK 495

Query: 554 NDTELQAWWGEIQEKGHPDK-REGWF-ALRTRDDLIKIASTIAWVGSGHHASVNFLQYAY 613
           +D ELQAWW EIQ+ GH DK +E W+ +L T  DLI I +TIAW+ SGHHA+VNF QY Y
Sbjct: 496 SDQELQAWWTEIQKVGHGDKYKEPWWPSLETPKDLIDIITTIAWIASGHHAAVNFAQYTY 555

Query: 614 GGYMPNRPTIARTNMLTENHSDKFLKDFINQPEKKLNELFPSDAQAAIVKQTMFLLSIHS 673
           GGY PNRPTIAR  M TE+ S +  ++F+  PE+ L E  PS  QA +V   + LLS HS
Sbjct: 556 GGYFPNRPTIARIKMPTEDPSKEEWENFLKNPEQTLLECLPSQIQATLVMVILNLLSNHS 615

Query: 674 PDEEYIGDAIEPAWALDPSISNAFEKFKANLTDLEKKIDELNQNKDLKNRYGAGIIPYEA 720
           PDEEYIG  +EP+WA + +I  +FE+F   L ++E  ID  N N +LKNR GAG++PYE 
Sbjct: 616 PDEEYIGQYMEPSWAENQTIKTSFERFNKRLKEIEGIIDSRNGNYNLKNRCGAGLVPYEL 675

BLAST of Cucsa.153640 vs. TrEMBL
Match: M5X8A3_PRUPE (Lipoxygenase OS=Prunus persica GN=PRUPE_ppa001631mg PE=3 SV=1)

HSP 1 Score: 629.0 bits (1621), Expect = 7.0e-177
Identity = 312/519 (60.12%), Postives = 382/519 (73.60%), Query Frame = 1

Query: 204 EFSEIKQRDQYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTPER 263
           E  EI  RD++ W  D EF+R TLAGLNPYSI+LV   P  SKLD   YGP ES  T E 
Sbjct: 275 ETPEIINRDRFAWFRDEEFSRQTLAGLNPYSIELVTEWPLKSKLDPEIYGPPESLITTEL 334

Query: 264 VQELLGCCITVGKALADKRLFVVDYHDTLMPYVRRVRKIKGTTLYGSRTLFFLNSDGTLM 323
           V++ +  C+TV +AL  K++F++DYHD  MPYV +VR+I+GTTLYGSRTL FL  DGTL 
Sbjct: 335 VEKEIRGCMTVDEALKRKKMFILDYHDLYMPYVNKVREIEGTTLYGSRTLLFLTEDGTLR 394

Query: 324 PLGIELTRPPMDEHLEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHC 383
           P+ IELTRPP+ ++  QWK+VFTP  ++T  WLWR AKAHV +HD+  HQLVIHWLR HC
Sbjct: 395 PVAIELTRPPVGDN-PQWKQVFTPTWDATGRWLWRLAKAHVCAHDAGYHQLVIHWLRTHC 454

Query: 384 CMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSAASYSVE 443
             EPY IA NRQLS MHPIYRLLHPHFRY M INA AR++LINAGG+IE++FS A YS+E
Sbjct: 455 ATEPYIIAANRQLSAMHPIYRLLHPHFRYTMEINALARESLINAGGVIESSFSPAKYSME 514

Query: 444 LSSSAYKE-WRFDMQALPEDLIHRGMVERKRDSEGRDVLELTIKDYPFANDGLLLWNALL 503
           LSS+AY + WRFDMQALP DLI RGM     D      L+LTI+DYPFANDGL+LW+A+ 
Sbjct: 515 LSSAAYDQLWRFDMQALPADLIRRGMAVE--DPTAEHGLKLTIEDYPFANDGLILWDAIK 574

Query: 504 EWVTEYVNHYYGDEENAVINDTELQAWWGEIQEKGHPDKREG--WFALRTRDDLIKIAST 563
           EWV +YVNHYY D    V +DTELQ WW E++ KGH DK++   W  L+T ++LI I +T
Sbjct: 575 EWVGDYVNHYYPDP-TLVESDTELQGWWTEVRTKGHADKKDEPWWPVLKTPENLIHILTT 634

Query: 564 IAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDKFLKDFINQPEKKLNELFP 623
           I WV +GHHA+VNF QY YGGY PN+PTIARTNM TEN S++F K+F+ +PE  L   FP
Sbjct: 635 IIWVTAGHHAAVNFGQYMYGGYFPNKPTIARTNMPTENPSEEFFKNFLKRPEMALLMCFP 694

Query: 624 SDAQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDPSISNAFEKFKANLTDLEKKIDEL 683
           S  QA  V   + +LS HSPDEEY+G+ +E +WA +P I+ AFE+F  NL  LE  IDE 
Sbjct: 695 SQIQATTVMAVLDVLSNHSPDEEYVGEKLESSWAENPVINAAFERFNGNLKRLEGIIDER 754

Query: 684 NQNKDLKNRYGAGIIPYEAMKPRSKPGITGSGVPYSVSI 720
           N N  LKNR GAG++PYE +KP S PG+TG GVP S+SI
Sbjct: 755 NTNLKLKNRVGAGVVPYELLKPFSTPGVTGMGVPNSISI 789

BLAST of Cucsa.153640 vs. TrEMBL
Match: M5X8A3_PRUPE (Lipoxygenase OS=Prunus persica GN=PRUPE_ppa001631mg PE=3 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 4.4e-38
Identity = 97/180 (53.89%), Postives = 115/180 (63.89%), Query Frame = 1

Query: 31  YIGEIVVAEGFGEIGAVIVELQEGVNERFIDTISVEAEEPPISVTFSCKSWVQPKGLIDH 90
           Y     +  GFG +GA+ VE  E  NE FI TI ++   P  SV   C SWV  K     
Sbjct: 30  YESNFTIPAGFGAVGAIEVE-NEHHNEIFIKTIDLQGF-PNGSVNVPCNSWVHAKFDNPQ 89

Query: 91  RRIFFSSNKSYLLGKTPRGLVKLREEDLANLRGEKEDGSVDRNERKAFERIYDYDLYNDL 150
           +RIFF+ NKSY+  +TP GL +LRE +L NLRG  E       ERK  +RIYDYD YNDL
Sbjct: 90  KRIFFT-NKSYIPSETPSGLKRLRELELENLRGNGE------GERKTSDRIYDYDTYNDL 149

Query: 151 GDPDLSMDLKRPVLGGSEEYPYPRRCRTGRPPTQNDPASEKRVEEWFYIPRDEEFSEIKQ 210
           GDPD   +L RPVL GS+E+PYPRRCRTGRP T+ DP SEKR     Y+PRDE FSE+KQ
Sbjct: 150 GDPDSKEELARPVL-GSKEHPYPRRCRTGRPRTKKDPLSEKRSSS-VYVPRDEAFSEVKQ 198

HSP 2 Score: 628.6 bits (1620), Expect = 9.2e-177
Identity = 310/519 (59.73%), Postives = 378/519 (72.83%), Query Frame = 1

Query: 204 EFSEIKQRDQYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTPER 263
           E  EI  RD++ W  D EF+R TLAGLNPYSI+LV   P  SKLD   YGP ES  T E 
Sbjct: 404 ETPEIVDRDKFAWFRDEEFSRQTLAGLNPYSIELVTEWPLKSKLDPEIYGPPESLITTEL 463

Query: 264 VQELLGCCITVGKALADKRLFVVDYHDTLMPYVRRVRKIKGTTLYGSRTLFFLNSDGTLM 323
           +++ +  C+TV +AL  K++F++DYHD LMPYV +VR+IKGTTLYGSRTLFFL  DGTL 
Sbjct: 464 IEKEIKGCMTVNEALERKKMFILDYHDLLMPYVNKVREIKGTTLYGSRTLFFLTEDGTLR 523

Query: 324 PLGIELTRPPMDEHLEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHC 383
           P+ IELTRPP+ +   QWK+VFTP  ++T  WLWR AKAHVL+HDS  HQLV+HWLR HC
Sbjct: 524 PVAIELTRPPVGDK-PQWKQVFTPTWDATGCWLWRLAKAHVLAHDSGYHQLVVHWLRTHC 583

Query: 384 CMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSAASYSVE 443
           C EPY IA  RQLS MHPIYRLLHPHFRY M INA AR++LINAGGIIE  FS   YS+E
Sbjct: 584 CTEPYIIAAYRQLSAMHPIYRLLHPHFRYTMEINALARESLINAGGIIEKCFSPGKYSME 643

Query: 444 LSSSAYKE-WRFDMQALPEDLIHRGMVERKRDSEGRDVLELTIKDYPFANDGLLLWNALL 503
           +SS AY + WRFDM+ALP DLI RGM     D      L+LTI+DYPFANDGL+LW+A+ 
Sbjct: 644 ISSVAYDQLWRFDMEALPADLIRRGMAVE--DPTAEHGLKLTIEDYPFANDGLMLWDAIK 703

Query: 504 EWVTEYVNHYYGDEENAVINDTELQAWWGEIQEKGHPDKREG--WFALRTRDDLIKIAST 563
           EWV++YVNHYY D  N + +DTELQ WW +++ KGH DK++   W  L+T + LI   +T
Sbjct: 704 EWVSDYVNHYYPDP-NLIESDTELQGWWTDVRTKGHADKKDEPWWPVLKTTESLIHALTT 763

Query: 564 IAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDKFLKDFINQPEKKLNELFP 623
           I WV +GHHA+VNF QY + GY PN PTIARTNM TE+ SD+F ++F+ +PE  L   FP
Sbjct: 764 IIWVTAGHHAAVNFGQYTFAGYFPNHPTIARTNMPTEDPSDEFFQNFMKKPEMALLMCFP 823

Query: 624 SDAQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDPSISNAFEKFKANLTDLEKKIDEL 683
           S  QA  V   + +LS HSPDEEYIGD++EPAWA +P I  A+E+F  NL  LE  IDE 
Sbjct: 824 SQIQATTVMAVIDVLSNHSPDEEYIGDSLEPAWAENPVIKAAYERFSGNLKRLEGIIDER 883

Query: 684 NQNKDLKNRYGAGIIPYEAMKPRSKPGITGSGVPYSVSI 720
           N N  LKNR GAG++PYE +KP S  G+TG GVP S+SI
Sbjct: 884 NTNMKLKNRVGAGVVPYELLKPFSTTGVTGMGVPNSISI 918

BLAST of Cucsa.153640 vs. TrEMBL
Match: S4UKZ4_MALDO (Lipoxygenase OS=Malus domestica GN=LOX5a PE=2 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 7.8e-35
Identity = 91/179 (50.84%), Postives = 113/179 (63.13%), Query Frame = 1

Query: 31  YIGEIVVAEGFGEIGAVIVELQEGVNERFIDTISVEAEEPPISVTFSCKSWVQPKGLIDH 90
           Y     +  GFG +GAV VE  E   E FI  I ++   P  +V   C SWV  K     
Sbjct: 154 YEATFTIPAGFGAVGAVQVE-NEHHKEIFIKNIDLDGF-PNGTVNIPCISWVHSKFDNSQ 213

Query: 91  RRIFFSSNKSYLLGKTPRGLVKLREEDLANLRGEKEDGSVDRNERKAFERIYDYDLYNDL 150
           +RIFF+ NKSY+  +TP GL KLRE +L  +RG   DG     +RK  +RIYDYD YND+
Sbjct: 214 KRIFFT-NKSYITSETPSGLKKLRESELQTMRG---DGG---GQRKTSDRIYDYDTYNDI 273

Query: 151 GDPDLSMDLKRPVLGGSEEYPYPRRCRTGRPPTQNDPASEKRVEEWFYIPRDEEFSEIK 210
           GDPD S DL RPVLGG +++PYPRRCRTGRP ++ DP SE+R     Y+PRDE FSE+K
Sbjct: 274 GDPDSSDDLARPVLGG-KDHPYPRRCRTGRPRSEKDPLSEQRSSS-VYVPRDEAFSEVK 321

HSP 2 Score: 628.2 bits (1619), Expect = 1.2e-176
Identity = 310/519 (59.73%), Postives = 378/519 (72.83%), Query Frame = 1

Query: 204 EFSEIKQRDQYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTPER 263
           E  EI  RD++ W  D EF+R TLAGLNPYSI+LV   P  SKLD   YGP ES  T E 
Sbjct: 404 ETPEIVDRDKFAWFRDEEFSRQTLAGLNPYSIELVTEWPLKSKLDPEIYGPPESLITTEL 463

Query: 264 VQELLGCCITVGKALADKRLFVVDYHDTLMPYVRRVRKIKGTTLYGSRTLFFLNSDGTLM 323
           +++ +  C+TV +AL  K++F++DYHD LMPYV +VR+IKGTTLYGSRTLFFL  DGTL 
Sbjct: 464 IEKEIKGCMTVNEALERKKMFILDYHDLLMPYVNKVREIKGTTLYGSRTLFFLTEDGTLR 523

Query: 324 PLGIELTRPPMDEHLEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHC 383
           P+ IELTRPP+ +   QWK+VFTP  ++T  WLWR AKAHVL+HDS  HQLV+HWLR HC
Sbjct: 524 PVAIELTRPPVGDK-PQWKQVFTPTWDATGCWLWRLAKAHVLAHDSGYHQLVVHWLRTHC 583

Query: 384 CMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSAASYSVE 443
           C EPY IA  RQLS MHPIYRLLHPHFRY M INA AR++LINAGGIIE  FS   YS+E
Sbjct: 584 CTEPYIIAAYRQLSAMHPIYRLLHPHFRYTMEINALARESLINAGGIIEKCFSPGKYSME 643

Query: 444 LSSSAYKE-WRFDMQALPEDLIHRGMVERKRDSEGRDVLELTIKDYPFANDGLLLWNALL 503
           +SS AY + WRFDM+ALP DLI RGM     D      L+LTI+DYPFANDGL+LW+A+ 
Sbjct: 644 ISSVAYDQLWRFDMEALPADLIRRGMAVE--DPTAEHGLKLTIEDYPFANDGLMLWDAIK 703

Query: 504 EWVTEYVNHYYGDEENAVINDTELQAWWGEIQEKGHPDKREG--WFALRTRDDLIKIAST 563
           EWV++YVNHYY D  N + +DTELQ WW +++ KGH DK++   W  L+T + LI   +T
Sbjct: 704 EWVSDYVNHYYPDP-NLIESDTELQGWWTDVRTKGHADKKDEPWWPVLKTTESLIHALTT 763

Query: 564 IAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDKFLKDFINQPEKKLNELFP 623
           I WV +GHHA+VNF QY + GY PN PTIARTNM TE+ SD+F ++F+ +PE  L   FP
Sbjct: 764 IIWVTAGHHAAVNFGQYTFAGYFPNHPTIARTNMPTEDPSDEFFQNFMKKPEMALLMCFP 823

Query: 624 SDAQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDPSISNAFEKFKANLTDLEKKIDEL 683
           S  QA  V   + +LS HSPDEEYIGD++EPAWA +P I  A+E+F  NL  LE  IDE 
Sbjct: 824 SQIQATTVMAVIDVLSNHSPDEEYIGDSLEPAWAENPVIKAAYERFSGNLKRLEGIIDER 883

Query: 684 NQNKDLKNRYGAGIIPYEAMKPRSKPGITGSGVPYSVSI 720
           N N  LKNR GAG++PYE +KP S  G+TG GVP S+SI
Sbjct: 884 NTNMKLKNRVGAGVVPYELLKPFSTHGVTGMGVPNSISI 918

BLAST of Cucsa.153640 vs. TAIR10
Match: AT3G45140.1 (AT3G45140.1 lipoxygenase 2)

HSP 1 Score: 599.4 bits (1544), Expect = 3.0e-171
Identity = 297/512 (58.01%), Postives = 367/512 (71.68%), Query Frame = 1

Query: 211 RDQYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTPERVQELLGC 270
           RD+++WL D EFAR TLAGLNPYSIQLV+  P +SKLD   YG   S  T E V+  +  
Sbjct: 389 RDRFSWLRDDEFARQTLAGLNPYSIQLVEEWPLISKLDPAVYGDPTSLITWEIVEREVKG 448

Query: 271 CITVGKALADKRLFVVDYHDTLMPYVRRVRKIKGTTLYGSRTLFFLNSDGTLMPLGIELT 330
            +TV +AL +KRLFV+DYHD L+PYV +VR++  TTLY SRTLFFL+ D TL P+ IELT
Sbjct: 449 NMTVDEALKNKRLFVLDYHDLLLPYVNKVRELNNTTLYASRTLFFLSDDSTLRPVAIELT 508

Query: 331 RPPMDEHLEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHCCMEPYAI 390
            PP + +  QWK+VFTPG ++T  WLW  AK H +SHD+  HQL+ HWLR H C EPY I
Sbjct: 509 CPP-NINKPQWKQVFTPGYDATSCWLWNLAKTHAISHDAGYHQLISHWLRTHACTEPYII 568

Query: 391 ATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSAASYSVELSSSAY- 450
           A NRQLS MHPIYRLLHPHFRY M INA AR++L+N GGIIE  F    Y++ELSS+ Y 
Sbjct: 569 AANRQLSAMHPIYRLLHPHFRYTMEINARARQSLVNGGGIIETCFWPGKYALELSSAVYG 628

Query: 451 KEWRFDMQALPEDLIHRGMVERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWVTEYV 510
           K WRFD + LP DLI RG+ E  + +E    + LTI DYPFANDGL+LW+A+ EWVT+YV
Sbjct: 629 KLWRFDQEGLPADLIKRGLAEEDKTAEHG--VRLTIPDYPFANDGLILWDAIKEWVTDYV 688

Query: 511 NHYYGDEENAVINDTELQAWWGEIQEKGHPDKREG--WFALRTRDDLIKIASTIAWVGSG 570
            HYY DEE  + +D ELQ WW E++  GH DK++   W  L+T+DDLI + +TIAWV SG
Sbjct: 689 KHYYPDEE-LITSDEELQGWWSEVRNIGHGDKKDEPWWPVLKTQDDLIGVVTTIAWVTSG 748

Query: 571 HHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDKFLKDFINQPEKKLNELFPSDAQAAI 630
           HHA+VNF QY YGGY PNRPT  R  M TE+ +D+ LK+F   PEK L + +PS  QA +
Sbjct: 749 HHAAVNFGQYGYGGYFPNRPTTTRIRMPTEDPTDEALKEFYESPEKVLLKTYPSQKQATL 808

Query: 631 VKQTMFLLSIHSPDEEYIGDAIEPAWALDPSISNAFEKFKANLTDLEKKIDELNQNKDLK 690
           V  T+ LLS HSPDEEYIG+  E +WA +P I+ AFE+FK  L  LE  IDE N N  LK
Sbjct: 809 VMVTLDLLSTHSPDEEYIGEQQEASWANEPVINAAFERFKGKLQYLEGVIDERNVNITLK 868

Query: 691 NRYGAGIIPYEAMKPRSKPGITGSGVPYSVSI 720
           NR GAG++ YE +KP S+ G+TG GVPYS+SI
Sbjct: 869 NRAGAGVVKYELLKPTSEHGVTGMGVPYSISI 896

HSP 2 Score: 160.2 bits (404), Expect = 4.7e-39
Identity = 87/181 (48.07%), Postives = 118/181 (65.19%), Query Frame = 1

Query: 29  KKYIGEIVVAEGFGEIGAVIVELQEGVNERFIDTISVEAEEPPISVTFSCKSWVQPKGLI 88
           +KY  E  + E FG +GA+ ++ Q     R +    VE + P  S+TF+C+SWV PK + 
Sbjct: 135 EKYECEFEMPEDFGPVGAIKIQNQY---HRQLFLKGVELKLPGGSITFTCESWVAPKSVD 194

Query: 89  DHRRIFFSSNKSYLLGKTPRGLVKLREEDLANLRGEKEDGSVDRNERKAFERIYDYDLYN 148
             +RIFFS +KSYL  +TP  L K R+E+L  L+G+  +   +  E   FERIYDYD+YN
Sbjct: 195 PTKRIFFS-DKSYLPSQTPEPLKKYRKEELETLQGKNRE---EVGEFTKFERIYDYDVYN 254

Query: 149 DLGDPDLSMDLKRPVLGGSEEYPYPRRCRTGRPPTQNDPASEKRVEEWFYIPRDEEFSEI 208
           D+GDPD   +L RPV+GG   +PYPRRC+TGR P + DP+SE+R    FY+PRDEEFS  
Sbjct: 255 DVGDPDNDPELARPVIGGLT-HPYPRRCKTGRKPCETDPSSEQRYGGEFYVPRDEEFSTA 307

Query: 209 K 210
           K
Sbjct: 315 K 307

BLAST of Cucsa.153640 vs. TAIR10
Match: AT1G67560.1 (AT1G67560.1 PLAT/LH2 domain-containing lipoxygenase family protein)

HSP 1 Score: 488.0 bits (1255), Expect = 9.8e-138
Identity = 252/516 (48.84%), Postives = 334/516 (64.73%), Query Frame = 1

Query: 212 DQYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTPERV-QELLGC 271
           D++ WL D EF R  LAG+NP +I+L+K LP  S LD   YGP+ES  T E + +E+   
Sbjct: 409 DRFAWLRDNEFGRQALAGVNPVNIELLKELPIRSNLDPALYGPQESVLTEEIIAREVEHY 468

Query: 272 CITVGKALADKRLFVVDYHDTLMPYVRRVRKIKGTT--LYGSRTLFFLNSDGTLMPLGIE 331
             T+ KAL +KRLF+VDYHD L+P+V ++  IK      Y SRT+FF + +G L PL IE
Sbjct: 469 GTTIEKALEEKRLFLVDYHDILLPFVEKINSIKEDPRKTYASRTIFFYSKNGALRPLAIE 528

Query: 332 LTRPPMDEHLEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHCCMEPY 391
           L+ PP  E   + K V+T G ++T  W+W+ AKAHV S+D+ +HQLV HWLR H  MEPY
Sbjct: 529 LSLPPTAE--SENKFVYTHGHDATTHWIWKLAKAHVCSNDAGVHQLVNHWLRTHASMEPY 588

Query: 392 AIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSAASYSVELSSSA 451
            IATNRQLSTMHP+Y+LLHPH RY + INA ARK+LIN GGIIE+ F+   Y++ELSS+A
Sbjct: 589 IIATNRQLSTMHPVYKLLHPHMRYTLEINARARKSLINGGGIIESCFTPGKYAMELSSAA 648

Query: 452 YKE-WRFDMQALPEDLIHRGMVERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWVTE 511
           YK  WRFDM+ LP DL+ RGM E   DS     + L I DYP+A DGLL+W A+ + V  
Sbjct: 649 YKSMWRFDMEGLPADLVRRGMAE--EDSSAECGVRLVIDDYPYAADGLLIWKAIKDLVES 708

Query: 512 YVNHYYGDEENAVINDTELQAWWGEIQEKGHPDKRE--GWFALRTRDDLIKIASTIAWVG 571
           YV H+Y D + ++ +D ELQAWW EI+ KGH DK++   W  L T  DL +I + + W+ 
Sbjct: 709 YVKHFYSDSK-SITSDLELQAWWDEIKNKGHYDKKDEPWWPKLNTTQDLSQILTNMIWIA 768

Query: 572 SGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDKFLKDFINQPEKKLNELFPSDAQA 631
           SG HA++NF QY +GGY+PNRPT+ R   L    +D   + F+  P+       P+  QA
Sbjct: 769 SGQHAAINFGQYPFGGYVPNRPTLLR--KLIPQETDPDYEMFMRNPQYSFLGSLPTQLQA 828

Query: 632 AIVKQTMFLLSIHSPDEEYIGD--AIEPAWALDPSISNAFEKFKANLTDLEKKIDELNQN 691
             V      LS HSPDEEY+ +   ++  W  D  +   F KF   L  +EK I+E N++
Sbjct: 829 TKVMAVQETLSTHSPDEEYLIELREVQRHWFQDEQVVKYFNKFSEELVKIEKTINERNKD 888

Query: 692 KDLKNRYGAGIIPYEAMKPRSKPGITGSGVPYSVSI 720
           K LKNR GAG+ PYE + P S  G+TG G+P S+SI
Sbjct: 889 KKLKNRTGAGMPPYELLLPTSPHGVTGRGIPNSISI 917

HSP 2 Score: 121.7 bits (304), Expect = 1.8e-27
Identity = 76/185 (41.08%), Postives = 101/185 (54.59%), Query Frame = 1

Query: 28  YKKYIGEIVVAEGFGEIGAVIVELQEGVNERFIDTISVEAEEPPISVTFSCKSWVQPKGL 87
           Y  +  +  V   FG+ GA++V     +    I    +  E+   ++ F   +W+  K  
Sbjct: 151 YLVFTADFTVPINFGKPGAILVT---NLLSTEICLSEIIIEDSTDTILFPANTWIHSKND 210

Query: 88  IDHRRIFFSSNKSYLLGKTPRGLVKLREEDLANLRGEKEDGSVDRNERKAFERIYDYDLY 147
               RI F S    L  +TP G+ +LRE+DL ++RG+       + ERK  ERIYDYD+Y
Sbjct: 211 NPQARIIFRSQPC-LPSETPDGIKELREKDLVSVRGD------GKGERKPHERIYDYDVY 270

Query: 148 NDLGDPDLSMDLKRPVLGGSEEYPYPRRCRTGRPPTQNDPASEKR--VEEWFYIPRDEEF 207
           NDLGDP    +  RPVL G  E PYPRRCRTGRP    DP  E R   +E FY+PRDE F
Sbjct: 271 NDLGDP-RKTERVRPVL-GVPETPYPRRCRTGRPLVSKDPPCESRGKEKEEFYVPRDEVF 323

Query: 208 SEIKQ 211
            EIK+
Sbjct: 331 EEIKR 323

BLAST of Cucsa.153640 vs. TAIR10
Match: AT1G72520.1 (AT1G72520.1 PLAT/LH2 domain-containing lipoxygenase family protein)

HSP 1 Score: 462.6 bits (1189), Expect = 4.4e-130
Identity = 243/518 (46.91%), Postives = 327/518 (63.13%), Query Frame = 1

Query: 207 EIKQRDQYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPR-ESKFTPERVQ 266
           +I  +D+Y WL D EFAR  +AG+NP +I+ V S P +S LD   YGP   S  T + + 
Sbjct: 415 KIVSKDKYAWLRDDEFARQAIAGINPVNIERVTSYPPVSNLDPEIYGPGLHSALTEDHII 474

Query: 267 ELLGCCITVGKALADKRLFVVDYHDTLMPYVRRVRKIKGTTLYGSRTLFFLNSDGTLMPL 326
             L   +TV +AL   RLF+VDYHD  +P++ R+  + G   Y +RT+ FL   GTL P+
Sbjct: 475 GQLDG-LTVQQALETNRLFMVDYHDIYLPFLDRINALDGRKAYATRTILFLTRLGTLKPI 534

Query: 327 GIELTRPPMDEHLEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHCCM 386
            IEL+ P      ++ K V TP  ++T  W+W+ AKAHV S+D+ +HQLV HWLR H C+
Sbjct: 535 AIELSLPSQSSSNQKSKRVVTPPVDATSNWMWQLAKAHVGSNDAGVHQLVNHWLRTHACL 594

Query: 387 EPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSAASYSVELS 446
           EP+ +A +RQLS MHPI++LL PH RY + INA AR+ LI+A G+IE+ F+A  Y +E+S
Sbjct: 595 EPFILAAHRQLSAMHPIFKLLDPHMRYTLEINAVARQTLISADGVIESCFTAGQYGLEIS 654

Query: 447 SSAYK-EWRFDMQALPEDLIHRGMVERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEW 506
           S+AYK +WRFDM+ LP DLI RGM     D      L+L ++DYP+ANDGLLLW+A+  W
Sbjct: 655 SAAYKNKWRFDMEGLPADLIRRGMA--VPDPTQPHGLKLLVEDYPYANDGLLLWSAIQTW 714

Query: 507 VTEYVNHYYGDEENAVINDTELQAWWGEIQEKGHPDKR--EGWFALRTRDDLIKIASTIA 566
           V  YV  YY +  N +  DTELQAW+ E    GH D R  E W  L T +DL+ + +TI 
Sbjct: 715 VRTYVERYYAN-SNLIQTDTELQAWYSESINVGHADHRDAEWWPKLSTVEDLVSVITTII 774

Query: 567 WVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDKFLKDFINQPEKKLNELFPSD 626
           W+ S  HA++NF QY YGGY+PNRP + R   L  + SD     FI  P+K      PS 
Sbjct: 775 WLASAQHAALNFGQYPYGGYVPNRPPLMR--RLIPDESDPEFTSFIEDPQKYFFSSMPSL 834

Query: 627 AQAAIVKQTMFLLSIHSPDEEYIGDAIEPA-WALDPSISNAFEKFKANLTDLEKKIDELN 686
            Q       +  LS HSPDEEYIG+  +P+ W  D  I +AF  F A +  +EK+ID+ N
Sbjct: 835 LQTTKFMAVVDTLSTHSPDEEYIGERQQPSIWTGDAEIVDAFYGFSAEIGRIEKEIDKRN 894

Query: 687 QNKDLKNRYGAGIIPYEAMKPRSKPGITGSGVPYSVSI 720
           ++   +NR GAG++PYE M P S+PG+T  GVP SVSI
Sbjct: 895 RDPSRRNRCGAGVLPYELMAPSSEPGVTCRGVPNSVSI 926

HSP 2 Score: 148.3 bits (373), Expect = 1.8e-35
Identity = 86/182 (47.25%), Postives = 112/182 (61.54%), Query Frame = 1

Query: 31  YIGEIVVAEGFGEIGAVIVELQEGVNERFIDTISVEAEEPPISVTFSCKSWVQPKGLIDH 90
           Y  E  V   FG  GA+ V   +   E F+++I++E       V F C SWVQ +     
Sbjct: 165 YTAEFTVDSAFGSPGAITVT-NKHQKEFFLESITIEGFACG-PVHFPCNSWVQSQKDHPS 224

Query: 91  RRIFFSSNKSYLLGKTPRGLVKLREEDLANLRGEKEDGSVDRNERKAFERIYDYDLYNDL 150
           +RI F+ N+ YL  +TP GL  LRE++L NLRG        + ERK  +RIYDYD+YND+
Sbjct: 225 KRILFT-NQPYLPSETPSGLRTLREKELENLRGN------GKGERKLSDRIYDYDVYNDI 284

Query: 151 GDPDLSMDLKRPVLGGSEEYPYPRRCRTGRPPTQNDPASEKRVEE--WFYIPRDEEFSEI 210
           G+PD+S +L RP LGG  E+PYPRRCRTGR  T  D  SE+RVE+    Y+PRDE+F E 
Sbjct: 285 GNPDISRELARPTLGG-REFPYPRRCRTGRSSTDTDMMSERRVEKPLPMYVPRDEQFEES 336

BLAST of Cucsa.153640 vs. TAIR10
Match: AT1G17420.1 (AT1G17420.1 lipoxygenase 3)

HSP 1 Score: 460.3 bits (1183), Expect = 2.2e-129
Identity = 240/517 (46.42%), Postives = 331/517 (64.02%), Query Frame = 1

Query: 207 EIKQRDQYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTPERVQE 266
           +I  +D+  WL D EFAR  +AG+NP +I+ VK+ P +S LD   YGP+ S  T + +  
Sbjct: 410 KILSKDKNAWLRDDEFARQAIAGINPVNIERVKTFPPVSNLDPKIYGPQHSALTDDHIIG 469

Query: 267 LLGCCITVGKALADKRLFVVDYHDTLMPYVRRVRKIKGTTLYGSRTLFFLNSDGTLMPLG 326
            L    +V +AL + RL+++DYHD  +P++ R+  + G   Y +RT+FFL   GTL P+ 
Sbjct: 470 HLDG-FSVQQALEENRLYMLDYHDIFLPFLDRINALDGRKAYATRTIFFLTRLGTLKPVA 529

Query: 327 IELTRPPMDEHLEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHCCME 386
           IEL+ PP      + K V TP  ++T  W+W+ AKAHV S+D+ +HQLV HWLR H C+E
Sbjct: 530 IELSLPPHGPK-HRSKRVLTPPVDATSNWMWQLAKAHVSSNDAGVHQLVNHWLRTHACLE 589

Query: 387 PYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSAASYSVELSS 446
           P+ +A +RQLS MHPI++LL PH RY + INA AR++LI+A G+IE  F+A +Y +E+S+
Sbjct: 590 PFILAAHRQLSAMHPIFKLLDPHMRYTLEINALARQSLISADGVIEGGFTAGAYGMEMSA 649

Query: 447 SAYK-EWRFDMQALPEDLIHRGMVERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWV 506
           +AYK  WRFDM+ LP DLI RGM     D+     L+L I+DYP+ANDGLLLW+A+  WV
Sbjct: 650 AAYKSSWRFDMEGLPADLIRRGMA--IPDATQPHGLKLLIEDYPYANDGLLLWSAIQTWV 709

Query: 507 TEYVNHYYGDEENAVINDTELQAWWGEIQEKGHPDKREG--WFALRTRDDLIKIASTIAW 566
             YV  YY    N +  D+ELQ+W+ E    GH D R+   W  L T DDL+ I +T+ W
Sbjct: 710 RTYVERYY-PNPNLIKTDSELQSWYSESINVGHADLRDADWWPELSTVDDLVSILTTLIW 769

Query: 567 VGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDKFLKDFINQPEKKLNELFPSDA 626
           + S  HA++NF QY YGGY+PNRP + R   L  + SD     FI+ PEK      PS A
Sbjct: 770 LASAQHAALNFGQYPYGGYVPNRPPLMR--RLIPDESDPEYASFISHPEKYYFSSMPSLA 829

Query: 627 QAAIVKQTMFLLSIHSPDEEYIGDAIEPA-WALDPSISNAFEKFKANLTDLEKKIDELNQ 686
           Q +     +  LS HSPDEEYIG+  +P+ W  D  I  AF  F A +  +EK+I++ N 
Sbjct: 830 QTSKFMAVVDTLSTHSPDEEYIGERQQPSIWTGDAEIVEAFYGFAAEIGRIEKEIEKRNA 889

Query: 687 NKDLKNRYGAGIIPYEAMKPRSKPGITGSGVPYSVSI 720
           + D +NR GAG++PYE + P S+PG+T  GVP SVSI
Sbjct: 890 DPDRRNRCGAGVLPYELLVPSSEPGVTCRGVPNSVSI 919

HSP 2 Score: 144.1 bits (362), Expect = 3.5e-34
Identity = 90/184 (48.91%), Postives = 116/184 (63.04%), Query Frame = 1

Query: 31  YIGEIVVAEGFGEIGAVIVELQEGVNERFIDTISVE--AEEPPISVTFSCKSWVQPKGLI 90
           Y  E  V   FG  GA+ V + +   E F+++I++E  A  P   V F C SWVQ +   
Sbjct: 159 YTAEFTVDAAFGSPGAITV-MNKHQKEFFLESITIEGFALGP---VHFPCNSWVQSQKDH 218

Query: 91  DHRRIFFSSNKSYLLGKTPRGLVKLREEDLANLRGEKEDGSVDRNERKAFERIYDYDLYN 150
             +RIFF+ N+ YL  +TP GL  LRE++L NLRG   DGS     RK  +RIYD+D+YN
Sbjct: 219 PDKRIFFT-NQPYLPNETPSGLRVLREKELKNLRG---DGS---GVRKLSDRIYDFDVYN 278

Query: 151 DLGDPDLSMDLKRPVLGGSEEYPYPRRCRTGRPPTQNDPASEKRVEE--WFYIPRDEEFS 210
           DLG+PD S +L RP LGG +E PYPRRCRTGR  T +D  +E RVE+    Y+PRDE+F 
Sbjct: 279 DLGNPDKSSELSRPKLGG-KEVPYPRRCRTGRQSTVSDKDAESRVEKPLPMYVPRDEQFE 330

BLAST of Cucsa.153640 vs. TAIR10
Match: AT1G55020.1 (AT1G55020.1 lipoxygenase 1)

HSP 1 Score: 417.5 bits (1072), Expect = 1.6e-116
Identity = 234/517 (45.26%), Postives = 313/517 (60.54%), Query Frame = 1

Query: 207 EIKQRDQYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTPERVQE 266
           ++ + D+  W +D EFAR  LAGLNP  IQL+K  P  SKLD   YG + S  T   ++ 
Sbjct: 351 QVIKEDKTAWRTDEEFAREMLAGLNPVVIQLLKEFPPKSKLDSESYGNQNSTITKSHIEH 410

Query: 267 LLGCCITVGKALADKRLFVVDYHDTLMPYVRRVRKIKGTTLYGSRTLFFLNSDGTLMPLG 326
            L   +TV +AL  +RLF++D+HDTLMPY+ RV     T  Y SRTL FL  DGTL PL 
Sbjct: 411 NLDG-LTVEEALEKERLFILDHHDTLMPYLGRVNTTT-TKTYASRTLLFLKDDGTLKPLV 470

Query: 327 IELTRP-PMDEHLEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHCCM 386
           IEL+ P P  +      EV+TPG    D  LW+ AKA V  +DS  HQL+ HW++ H  +
Sbjct: 471 IELSLPHPNGDKFGAVSEVYTPGEGVYDS-LWQLAKAFVGVNDSGNHQLISHWMQTHASI 530

Query: 387 EPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSAASYSVELS 446
           EP+ IATNRQLS +HP+++LL PHFR  M INA AR+ LIN GGI E     + Y++E+S
Sbjct: 531 EPFVIATNRQLSVLHPVFKLLEPHFRDTMNINALARQILINGGGIFEITVFPSKYAMEMS 590

Query: 447 SSAYK-EWRFDMQALPEDLIHRGMVERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEW 506
           S  YK  W F  QALP +L  RGM     D E    L L IKDYP+A DGL +W A+  W
Sbjct: 591 SFIYKNHWTFPDQALPAELKKRGMA--VEDPEAPHGLRLRIKDYPYAVDGLEVWYAIESW 650

Query: 507 VTEYVNHYYGDEENAVINDTELQAWWGEIQEKGHPDKRE--GWFALRTRDDLIKIASTIA 566
           V +Y+  +Y  EE+ +  DTELQAWW E++E+GH DK+    W  ++TR++L++  + I 
Sbjct: 651 VRDYIFLFYKIEED-IQTDTELQAWWKEVREEGHGDKKSEPWWPKMQTREELVESCTIII 710

Query: 567 WVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDKFLKDFINQPEKKLNELFPSD 626
           WV S  HA+VNF QY   GY+PNRPTI+R  M  EN  +   ++    P+K   +   + 
Sbjct: 711 WVASALHAAVNFGQYPVAGYLPNRPTISRQYMPKENTPE--FEELEKNPDKVFLKTITAQ 770

Query: 627 AQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDPSISNAFEKFKANLTDLEKKIDELNQ 686
            Q  +    + +LS HS DE Y+G      WA +     AFEKF   + ++EK IDE N 
Sbjct: 771 LQTLLGISLIEILSTHSSDEVYLGQRDSKEWAAEKEALEAFEKFGEKVKEIEKNIDERND 830

Query: 687 NKDLKNRYGAGIIPYEAMKPRSKPGITGSGVPYSVSI 720
           ++ LKNR G   +PY  + P S+ G+TG G+P SVSI
Sbjct: 831 DETLKNRTGLVKMPYTLLFPSSEGGVTGRGIPNSVSI 859

HSP 2 Score: 125.6 bits (314), Expect = 1.3e-28
Identity = 74/176 (42.05%), Postives = 101/176 (57.39%), Query Frame = 1

Query: 41  FGEIGAVIVELQEGVNERFIDTISVEAEEPPISVTFSCKSWVQPKGLIDHRRIFFSSNKS 100
           FG  GA ++      +E  + ++++E       V + C SW+ P       R+FFS NK+
Sbjct: 107 FGYPGAFLIR-NSHFSEFLLKSLTLEDVPGHGRVHYICNSWIYPAKHYTTDRVFFS-NKT 166

Query: 101 YLLGKTPRGLVKLREEDLANLRGEKEDGSVDRNERKAFERIYDYDLYNDLGDPDLSMDLK 160
           YL  +TP  L+K REE+L +LRG  E       E K ++R+YDY  YNDLG P  +    
Sbjct: 167 YLPHETPATLLKYREEELVSLRGTGE------GELKEWDRVYDYAYYNDLGVPPKN---P 226

Query: 161 RPVLGGSEEYPYPRRCRTGRPPTQNDPASEKRVEEW----FYIPRDEEFSEIKQRD 213
           RPVLGG++EYPYPRR RTGR PT+ DP +E R+        Y+PRDE F  +K  D
Sbjct: 227 RPVLGGTQEYPYPRRGRTGRKPTKEDPQTESRLPITSSLDIYVPRDERFGHLKMSD 271

BLAST of Cucsa.153640 vs. NCBI nr
Match: gi|778693093|ref|XP_004142238.2| (PREDICTED: lipoxygenase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 1045.0 bits (2701), Expect = 5.9e-302
Identity = 506/513 (98.64%), Postives = 507/513 (98.83%), Query Frame = 1

Query: 207 EIKQRDQYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTPERVQE 266
           E  +RDQYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTPERVQE
Sbjct: 311 ESYKRDQYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTPERVQE 370

Query: 267 LLGCCITVGKALADKRLFVVDYHDTLMPYVRRVRKIKGTTLYGSRTLFFLNSDGTLMPLG 326
           LLGCCITVGKALADKRLFVVDYHDTLMPYVRRVRKIKGTTLYGSRTLFFLNSDGTLMPLG
Sbjct: 371 LLGCCITVGKALADKRLFVVDYHDTLMPYVRRVRKIKGTTLYGSRTLFFLNSDGTLMPLG 430

Query: 327 IELTRPPMDEHLEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHCCME 386
           IELTRPPMDEHLEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHCCME
Sbjct: 431 IELTRPPMDEHLEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHCCME 490

Query: 387 PYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSAASYSVELSS 446
           PYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSAASYSVELSS
Sbjct: 491 PYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSAASYSVELSS 550

Query: 447 SAYKEWRFDMQALPEDLIHRGMVERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWVT 506
           SAYKEWRFDMQALPEDLIHRGM ERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWVT
Sbjct: 551 SAYKEWRFDMQALPEDLIHRGMAERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWVT 610

Query: 507 EYVNHYYGDEENAVINDTELQAWWGEIQEKGHPDKREGWFALRTRDDLIKIASTIAWVGS 566
           EYVNHYYGDEENAVINDTELQAWWGEIQEKGHPDKREGWFALRTRDDLIKIASTIAWVGS
Sbjct: 611 EYVNHYYGDEENAVINDTELQAWWGEIQEKGHPDKREGWFALRTRDDLIKIASTIAWVGS 670

Query: 567 GHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDKFLKDFINQPEKKLNELFPSDAQAA 626
           GHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDKFLKDFINQPEKKLNELFPSDAQAA
Sbjct: 671 GHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDKFLKDFINQPEKKLNELFPSDAQAA 730

Query: 627 IVKQTMFLLSIHSPDEEYIGDAIEPAWALDPSISNAFEKFKANLTDLEKKIDELNQNKDL 686
           IVKQTMFLLSIHSPDEEYIGDAIEPAWALD SIS AFEKFKANLTDLEKKIDELNQNKDL
Sbjct: 731 IVKQTMFLLSIHSPDEEYIGDAIEPAWALDQSISKAFEKFKANLTDLEKKIDELNQNKDL 790

Query: 687 KNRYGAGIIPYEAMKPRSKPGITGSGVPYSVSI 720
           KNRYGA IIPYEAMKPRSKPGITGSGVPYSVSI
Sbjct: 791 KNRYGAAIIPYEAMKPRSKPGITGSGVPYSVSI 823

BLAST of Cucsa.153640 vs. NCBI nr
Match: gi|778693093|ref|XP_004142238.2| (PREDICTED: lipoxygenase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 418.3 bits (1074), Expect = 2.7e-113
Identity = 203/206 (98.54%), Postives = 206/206 (100.00%), Query Frame = 1

Query: 5   TDEQPKEFIKCEAQLQEVDELVEYKKYIGEIVVAEGFGEIGAVIVELQEGVNERFIDTIS 64
           ++EQPKEFIKCEAQLQEVDELVEYKKYIGEIVVAEGFGEIGAVIVELQEGVNERFIDTIS
Sbjct: 36  SNEQPKEFIKCEAQLQEVDELVEYKKYIGEIVVAEGFGEIGAVIVELQEGVNERFIDTIS 95

Query: 65  VEAEEPPISVTFSCKSWVQPKGLIDHRRIFFSSNKSYLLGKTPRGLVKLREEDLANLRGE 124
           VEAEEPPIS+TFSCKSWVQPKGLIDHRRIFFSSNKSYLLGKTPRGLVKLREEDLANLRGE
Sbjct: 96  VEAEEPPISITFSCKSWVQPKGLIDHRRIFFSSNKSYLLGKTPRGLVKLREEDLANLRGE 155

Query: 125 KEDGSVDRNERKAFERIYDYDLYNDLGDPDLSMDLKRPVLGGSEEYPYPRRCRTGRPPTQ 184
           KEDGSVDRNERKAFERIYDYDLYNDLGDPDLSMDLKRPVLGGSEEYPYPRRCRTGRPPTQ
Sbjct: 156 KEDGSVDRNERKAFERIYDYDLYNDLGDPDLSMDLKRPVLGGSEEYPYPRRCRTGRPPTQ 215

Query: 185 NDPASEKRVEEWFYIPRDEEFSEIKQ 211
           NDPASEKRVEEWFYIPRDEEFSEIKQ
Sbjct: 216 NDPASEKRVEEWFYIPRDEEFSEIKQ 241

HSP 2 Score: 979.5 bits (2531), Expect = 3.0e-282
Identity = 484/542 (89.30%), Postives = 494/542 (91.14%), Query Frame = 1

Query: 180 RPPT--QNDPASEKRVEEWFYIPRDEEFSEIKQRDQYNWLSDIEFARLTLAGLNPYSIQL 239
           RPP    +D  S   VE    +P  E +    +RD+YNWLSDIEFARLTL GLNPYSIQL
Sbjct: 288 RPPALKSSDTPSSTLVE----LPPPESY----KRDKYNWLSDIEFARLTLVGLNPYSIQL 347

Query: 240 VKSLPFMSKLDEGDYGPRESKFTPERVQELLGCCITVGKALADKRLFVVDYHDTLMPYVR 299
           VKSLPFMSKLDEGDY PRESKFTPE VQELLGC ITV KALADKRLFVVDYHDTLMPYV 
Sbjct: 348 VKSLPFMSKLDEGDYRPRESKFTPEXVQELLGCYITVDKALADKRLFVVDYHDTLMPYVS 407

Query: 300 RVRKIKGTTLYGSRTLFFLNSDGTLMPLGIELTRPPMDEHLEQWKEVFTPGTNSTDLWLW 359
           +VRKIKGTTLYGSRTLFFLNSDG LMPLGIELTRPPMD HLEQWKEVFTPGTNSTD+WLW
Sbjct: 408 KVRKIKGTTLYGSRTLFFLNSDGILMPLGIELTRPPMDGHLEQWKEVFTPGTNSTDVWLW 467

Query: 360 RFAKAHVLSHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRIN 419
           R AKAHVLSHDSCIHQLVIHWLRAHCCME YAIATNRQLSTMHPIYRLLHPHFRYNMRIN
Sbjct: 468 RLAKAHVLSHDSCIHQLVIHWLRAHCCMESYAIATNRQLSTMHPIYRLLHPHFRYNMRIN 527

Query: 420 ANARKNLINAGGIIENAFSAASYSVELSSSAYKEWRFDMQALPEDLIHRGMVERKRDSEG 479
           ANARKNLINA GIIENAFS ASYSVELSSSAYK WRFD QALPEDLIHRGM ERKRDSEG
Sbjct: 528 ANARKNLINARGIIENAFSGASYSVELSSSAYKAWRFDEQALPEDLIHRGMAERKRDSEG 587

Query: 480 RDVLELTIKDYPFANDGLLLWNALLEWVTEYVNHYYGDEENAVINDTELQAWWGEIQEKG 539
           RDVLELTIKDYPFANDGLLLWNALLEWVTEYVNHYYGDEENAV ND ELQAWWGEIQEKG
Sbjct: 588 RDVLELTIKDYPFANDGLLLWNALLEWVTEYVNHYYGDEENAVTNDKELQAWWGEIQEKG 647

Query: 540 HPDKREGWFALRTRDDLIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTE 599
           HPDKREGW ALRTRDDLIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTE
Sbjct: 648 HPDKREGWPALRTRDDLIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTE 707

Query: 600 NHSDKFLKDFINQPEKKLNELFPSDAQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDP 659
           NHSD+ LKDF N PE KLNELFPS  QAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALD 
Sbjct: 708 NHSDEILKDFRNYPENKLNELFPSVVQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDQ 767

Query: 660 SISNAFEKFKANLTDLEKKIDELNQNKDLKNRYGAGIIPYEAMKPRSKPGITGSGVPYSV 719
           SIS AFEKFKANLTDLEKKIDELN+NKDLKNRYGAGIIPYE MKPRSKPG+TG GVPYSV
Sbjct: 768 SISKAFEKFKANLTDLEKKIDELNENKDLKNRYGAGIIPYEVMKPRSKPGVTGIGVPYSV 821

BLAST of Cucsa.153640 vs. NCBI nr
Match: gi|778693100|ref|XP_011653578.1| (PREDICTED: LOW QUALITY PROTEIN: lipoxygenase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 389.4 bits (999), Expect = 1.3e-104
Identity = 188/206 (91.26%), Postives = 198/206 (96.12%), Query Frame = 1

Query: 5   TDEQPKEFIKCEAQLQEVDELVEYKKYIGEIVVAEGFGEIGAVIVELQEGVNERFIDTIS 64
           ++EQPKEFIKCEAQLQEVDE VEYKKY G+I VAEGFG+IGAVIVELQ+GVNERF+DTIS
Sbjct: 36  SNEQPKEFIKCEAQLQEVDESVEYKKYKGDIKVAEGFGKIGAVIVELQDGVNERFVDTIS 95

Query: 65  VEAEEPPISVTFSCKSWVQPKGLIDHRRIFFSSNKSYLLGKTPRGLVKLREEDLANLRGE 124
           VEAE+PPISVTFSCKSWVQPKGLIDHRRIFFSSNKSYL GKTP GLVKLREEDLANLRGE
Sbjct: 96  VEAEKPPISVTFSCKSWVQPKGLIDHRRIFFSSNKSYLSGKTPGGLVKLREEDLANLRGE 155

Query: 125 KEDGSVDRNERKAFERIYDYDLYNDLGDPDLSMDLKRPVLGGSEEYPYPRRCRTGRPPTQ 184
           K DGSVDRNERKAFERIYDYDLYNDLGDPD SM+LKRPVLGGSEEYPYPRRCRTGRP TQ
Sbjct: 156 KADGSVDRNERKAFERIYDYDLYNDLGDPDQSMNLKRPVLGGSEEYPYPRRCRTGRPLTQ 215

Query: 185 NDPASEKRVEEWFYIPRDEEFSEIKQ 211
           NDPASEKR+EEWFY+PRDEEFSEIKQ
Sbjct: 216 NDPASEKRIEEWFYVPRDEEFSEIKQ 241

HSP 2 Score: 970.7 bits (2508), Expect = 1.4e-279
Identity = 476/542 (87.82%), Postives = 496/542 (91.51%), Query Frame = 1

Query: 180 RPP--TQNDPASEKRVEEWFYIPRDEEFSEIKQRDQYNWLSDIEFARLTLAGLNPYSIQL 239
           RPP  + +D  S   VE    +P  E +    +RDQYNWLSDIEFARLTLAGLNPYSIQL
Sbjct: 66  RPPALSSSDVPSSTLVE----LPPPESY----KRDQYNWLSDIEFARLTLAGLNPYSIQL 125

Query: 240 VKSLPFMSKLDEGDYGPRESKFTPERVQELLGCCITVGKALADKRLFVVDYHDTLMPYVR 299
           V+ LPF S L+E DYGPR+SKFTPERV ELLGC ITV KAL DKRLFV+DYHDTL+PYVR
Sbjct: 126 VRDLPFKSTLEESDYGPRQSKFTPERVHELLGCRITVAKALVDKRLFVLDYHDTLLPYVR 185

Query: 300 RVRKIKGTTLYGSRTLFFLNSDGTLMPLGIELTRPPMDEHLEQWKEVFTPGTNSTDLWLW 359
           +VRKIKGTTLYGSRTLFFLNSDGTLMPLGIELTRPPMD HLEQWKEVFTPGT+STD+WLW
Sbjct: 186 KVRKIKGTTLYGSRTLFFLNSDGTLMPLGIELTRPPMDGHLEQWKEVFTPGTDSTDVWLW 245

Query: 360 RFAKAHVLSHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRIN 419
           R AKAHVLSHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRIN
Sbjct: 246 RLAKAHVLSHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRIN 305

Query: 420 ANARKNLINAGGIIENAFSAASYSVELSSSAYKEWRFDMQALPEDLIHRGMVERKRDSEG 479
           ANARKNLINAGGIIE  FSAASYSVELSSSAYKEWRFD QALPEDLIHRGM E K DS G
Sbjct: 306 ANARKNLINAGGIIERTFSAASYSVELSSSAYKEWRFDKQALPEDLIHRGMAEIKTDSSG 365

Query: 480 RDVLELTIKDYPFANDGLLLWNALLEWVTEYVNHYYGDEENAVINDTELQAWWGEIQEKG 539
           RDV ELTIKDYPFANDGLLLWNALLEWVTEYVNHYYGD++ AV ND ELQAWW EIQ+KG
Sbjct: 366 RDVFELTIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDKYAVKNDKELQAWWEEIQKKG 425

Query: 540 HPDKREGWFALRTRDDLIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTE 599
           HPDK+ GW ALRTRDDLIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRP+IARTNMLTE
Sbjct: 426 HPDKKTGWPALRTRDDLIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRPSIARTNMLTE 485

Query: 600 NHSDKFLKDFINQPEKKLNELFPSDAQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDP 659
           NHSDKFLKDFINQPEKKL+ELFPSDAQAA+VKQTMFLLSIHSPDEEYIGDAIEPAWALDP
Sbjct: 486 NHSDKFLKDFINQPEKKLHELFPSDAQAALVKQTMFLLSIHSPDEEYIGDAIEPAWALDP 545

Query: 660 SISNAFEKFKANLTDLEKKIDELNQNKDLKNRYGAGIIPYEAMKPRSKPGITGSGVPYSV 719
           SIS AFEKFKANLT LEKKIDELNQNKDLKNRYGAGIIPYE MKPRSKPGITGSGVPYSV
Sbjct: 546 SISEAFEKFKANLTVLEKKIDELNQNKDLKNRYGAGIIPYEVMKPRSKPGITGSGVPYSV 599

BLAST of Cucsa.153640 vs. NCBI nr
Match: gi|659098040|ref|XP_008449947.1| (PREDICTED: lipoxygenase 2, chloroplastic-like, partial [Cucumis melo])

HSP 1 Score: 42.0 bits (97), Expect = 5.3e+00
Identity = 16/18 (88.89%), Postives = 18/18 (100.00%), Query Frame = 1

Query: 193 VEEWFYIPRDEEFSEIKQ 211
           +EEWFY+PRDEEFSEIKQ
Sbjct: 1   IEEWFYVPRDEEFSEIKQ 18

HSP 2 Score: 863.2 bits (2229), Expect = 3.2e-247
Identity = 430/568 (75.70%), Postives = 473/568 (83.27%), Query Frame = 1

Query: 157 MDLKRPVLGGS-EEYPY--PRRCRTGRPPTQNDPASEKRVEEWFYIPRDEEFSEIKQRDQ 216
           +D+K P       + P+  P    + +PP  N    E        +P   + S     D+
Sbjct: 228 LDIKTPFAASKINQLPFDIPSLVSSNQPPPLNSLDDEPSSSVELPLPESYKISG----DK 287

Query: 217 YNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTPERVQELLGCCIT 276
           YNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFT E VQELLGC I 
Sbjct: 288 YNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTHELVQELLGCRIK 347

Query: 277 VGKALADKRLFVVDYHDTLMPYVRRVRKIKGTTLYGSRTLFFLNSDGTLMPLGIELTRPP 336
           V KAL DKRLFV+DYHD LMPYV +VRKI+ TTLYGSRTLFFLNSD TL PLGIELTRPP
Sbjct: 348 VCKALTDKRLFVLDYHDILMPYVSKVRKIEWTTLYGSRTLFFLNSDDTLEPLGIELTRPP 407

Query: 337 MDEHLEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHCCMEPYAIATN 396
           +  H EQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHCCMEPYAIAT+
Sbjct: 408 IKGHSEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHCCMEPYAIATH 467

Query: 397 RQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSAASYSVELSSSAYKEWR 456
           RQLSTMHPIYRLLHPHFRY MRINANARK LI AGGIIE  FSAA +S+ELSSSAYKEWR
Sbjct: 468 RQLSTMHPIYRLLHPHFRYTMRINANARKILICAGGIIEGTFSAAYHSMELSSSAYKEWR 527

Query: 457 FDMQALPEDLIHRGMVERKRDSEGRDVLE-LTIKDYPFANDGLLLWNALLEWVTEYVNHY 516
           FD+Q LP DL+HRGM E K+D+E  +V++ LTIKDYPFANDGL LWNAL EWVTEYVNHY
Sbjct: 528 FDLQGLPYDLVHRGMAETKKDAECHEVIDKLTIKDYPFANDGLQLWNALWEWVTEYVNHY 587

Query: 517 YGDEENAVINDTELQAWWGEIQEKGHPDKREGWFALRTRDDLIKIASTIAWVGSGHHASV 576
           Y  + NAV ND ELQAWW EI++KGHPD +EGW  L T++DLIKIASTIAWVGSGHHAS+
Sbjct: 588 YKCDMNAVTNDKELQAWWKEIKDKGHPDIKEGWPKLETKEDLIKIASTIAWVGSGHHASI 647

Query: 577 NFLQYAYGGYMPNRPTIARTNMLTENH-SDKFLKDFINQPEKKLNELFPSDAQAAIVKQT 636
           NFLQYAY  Y+PNRP++AR NMLTEN+ S K  KDF+NQPE KL ELFP+D QA IV+QT
Sbjct: 648 NFLQYAYXSYIPNRPSVARANMLTENNPSGKISKDFLNQPEDKLKELFPTDEQATIVQQT 707

Query: 637 MFLLSIHSPDEEYIGDAIEPAWALDPSISNAFEKFKANLTDLEKKIDELNQNKDLKNRYG 696
           MF+LSIHS DEEYIGD IEPAWAL+P IS AFE+FKA LTDLEK+I+ELN+NKDLKNR G
Sbjct: 708 MFVLSIHSSDEEYIGDDIEPAWALEPCISKAFERFKAKLTDLEKRINELNENKDLKNRCG 767

Query: 697 AGIIPYEAMKPRSKPGITGSGVPYSVSI 720
           AGIIPYEAMKPRSKPGITGSGVPYSVSI
Sbjct: 768 AGIIPYEAMKPRSKPGITGSGVPYSVSI 791

BLAST of Cucsa.153640 vs. NCBI nr
Match: gi|778697632|ref|XP_011654363.1| (PREDICTED: LOW QUALITY PROTEIN: lipoxygenase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 261.2 bits (666), Expect = 5.5e-66
Identity = 147/251 (58.57%), Postives = 173/251 (68.92%), Query Frame = 1

Query: 1   MYIYTDEQPKEFIKCEAQLQ---EVDELVEYKKYIGEIVVAEGFGEIGAVIVELQEGVNE 60
           MYIYT E  ++ I C A L+   +V +L E       I V   FGEIGAVIVEL + V E
Sbjct: 1   MYIYT-EGNQQIIGCTAGLKCGGDVSKLFETS-----IDVPGDFGEIGAVIVELDDDVKE 60

Query: 61  RFIDTISVEAEEPPISVTFSCKSWVQPKGLIDHRRIFFSSNKSYLLGKTPRGLVKLREED 120
           RFIDTI+++ ++  + +TFSCKSWVQPK     RRIFFSSNKSYL GKTP GLVKLR ED
Sbjct: 61  RFIDTITIDFKKCTL-LTFSCKSWVQPKSSTHQRRIFFSSNKSYLSGKTPEGLVKLRAED 120

Query: 121 LANLRGEKEDGSVDRNERKAFERIYDYDLYNDLGDPDLSMDLKRPVLGGSEEYPYPRRCR 180
           LANLRGEKEDGSVD+ +R AFERIYDYDLYNDLG PD S DL RPVLGGSEEYPYPRRCR
Sbjct: 121 LANLRGEKEDGSVDKKKRNAFERIYDYDLYNDLGKPDESSDLIRPVLGGSEEYPYPRRCR 180

Query: 181 TGRPPTQNDPASEKRVEEWFYIPRDEEFSEIKQ----RDQYNWLSDIEFARLTLAGL-NP 240
           TGR  + ND  +E+R+E  FY+PRDE FSE KQ     +    L    F+ L    +  P
Sbjct: 181 TGRDTSDNDKHAEERIEAPFYVPRDENFSEEKQGSVLAENEALLGKAPFSDLPELDIKTP 240

Query: 241 YSIQLVKSLPF 244
           ++   +  LPF
Sbjct: 241 FAASKINQLPF 244

HSP 2 Score: 837.4 bits (2162), Expect = 1.9e-239
Identity = 403/513 (78.56%), Postives = 446/513 (86.94%), Query Frame = 1

Query: 207 EIKQRDQYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTPERVQE 266
           E  +RD++ WLSDIEFARLTLAGLNPYSIQLV++LPF SKLD   YGP +SKFT  RVQE
Sbjct: 387 EALKRDKFLWLSDIEFARLTLAGLNPYSIQLVRNLPFKSKLDVTKYGPPDSKFTLTRVQE 446

Query: 267 LLGCCITVGKALADKRLFVVDYHDTLMPYVRRVRKIKGTTLYGSRTLFFLNSDGTLMPLG 326
           LLGC + V  AL  KRLFV+DYHDTLMPYV++VR+I+  TLYGSRTLFFLNSD TL+PLG
Sbjct: 447 LLGCRMNVADALKKKRLFVLDYHDTLMPYVQKVREIEDKTLYGSRTLFFLNSDDTLVPLG 506

Query: 327 IELTRPPMDEHLEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHCCME 386
           IELTRPP+    +QWKE+F PGTNSTD+WLWR AKAHVLSHDSCIHQLVIHWLRAHCCME
Sbjct: 507 IELTRPPIKGDPKQWKEIFPPGTNSTDVWLWRLAKAHVLSHDSCIHQLVIHWLRAHCCME 566

Query: 387 PYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGIIENAFSAASYSVELSS 446
           PYAIATNR+LSTMHPIYRLLHPHFRY MRINANARKNLI+AGGIIE+ FS ASYSVELSS
Sbjct: 567 PYAIATNRRLSTMHPIYRLLHPHFRYTMRINANARKNLISAGGIIEDTFSTASYSVELSS 626

Query: 447 SAYKEWRFDMQALPEDLIHRGMVERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWVT 506
            AYKEWRFD+Q LP+DL+HRGM ERK D  GRDV ELTIKDYPFANDGLLLW+AL +WVT
Sbjct: 627 LAYKEWRFDLQGLPDDLVHRGMAERKTDPSGRDVFELTIKDYPFANDGLLLWDALWQWVT 686

Query: 507 EYVNHYYGDEENAVINDTELQAWWGEIQEKGHPDKREGWFALRTRDDLIKIASTIAWVGS 566
           +YVNHYY D ENAVIND ELQAWW EIQ+KGHPD +EGW  L T++ LIKIASTIAWVGS
Sbjct: 687 KYVNHYYADAENAVINDEELQAWWKEIQDKGHPDIKEGWPKLETKEHLIKIASTIAWVGS 746

Query: 567 GHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDKFLKDFINQPEKKLNELFPSDAQAA 626
           GHHASVNFLQYAYGGY+PNRP+IAR NMLTEN S    K+F+NQPE+KL +LFP++ QA 
Sbjct: 747 GHHASVNFLQYAYGGYIPNRPSIARANMLTENRSVSGRKEFLNQPEEKLKKLFPTEDQAT 806

Query: 627 IVKQTMFLLSIHSPDEEYIGDAIEPAWALDPSISNAFEKFKANLTDLEKKIDELNQNKDL 686
            V +TMFLLS+HSPDEEYIGD IEPAW LD SISNAFE+FK  L  LE KIDELNQN+DL
Sbjct: 807 KVMKTMFLLSMHSPDEEYIGDDIEPAWDLDQSISNAFEEFKTKLMMLENKIDELNQNEDL 866

Query: 687 KNRYGAGIIPYEAMKPRSKPGITGSGVPYSVSI 720
           KNR GAGIIPYEAMKPRS PGITG GVPYS+SI
Sbjct: 867 KNRNGAGIIPYEAMKPRSNPGITGIGVPYSISI 899