Cucsa.144210 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.144210
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionCa-2+ dependent nuclease
Locationscaffold01080 : 628232 .. 630374 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GGCATGTCAAACTCACACTTAATCAAGTTTTGTCTCATGGGCAACAGGGACTATTGACATTCTATGGTCTTCCTCTTCCTCATACTCTCGTCAAACCTTCTGCTCCGGTTCCAACTGCCGTCTCGATACCTGACGGAGTCAAGTTTGAGCTGCAGACGCTGCCAGTAAGATACTTCTATGGGAAATGACCAACCATTTTGATCATAACGTGTACGTTCTCATTGTGTTAAATTCATACTAGGTTGATGCCAAAGCTGTAGCAGATGGTGATACAGTGACAGTGTATGTGAGCACATCACACCCGAGAGAGTCCTCTTGTGTTCCAAAAGAGGTTAGAAATGCAGCCCTTCAAAGATCACGAGCTAGAAACGCAAAGAACTTTGCAAAGGCCGATGCTCTTCACAAGACAATCACGGATGCTGGTTACAGGTATATGATCTCTATCTCTACTATATATAATTGGAATTTATAAATAGATTGCTGAGATTCAATGCCTGTTTCTTCTTCTTCGAAAGGGTAATTACTTTTCAGAATGAGGAGGTTCTTGCAAAAAAGTACAGAATTCGTCTCAGGTAATTAAAAATTTTAAATTAGCTCTATTTGCATTAGATGCTTTAGACCCTATATTTTGATCATCGTTTGTCCTTTCTGACTCATAAACTCTCAGTGCGTTTATTTTAGTTTATGTAAATTTTAAATGTTGTCTCCTTTGATCTAACCGTTCTTTTGAATCTGGAGTTTTCGTTTGGTTTTTTGTACACTCAAATTTTGAGTTTGACTAATACAACCATTTTATTTTTGAAATTTGGATTTTTGTTTTTGTTTCATATCTAGGTTTCAACATTTATATCTTTAACTTTAATTTTCATTTCAAACCCACTTCTATTCATTAATTAAGATGTTTAAAAATATCTATTAATTAATGTTATAAACTTTTTTATTAATTAATGTTAGAAAATTTTAATTTGACAAAATATTTACTAACTTATTGTAAAAATTCAGATTCTATGACATTGATAGACAGTTTATCAGTTTCTATTTTGATAGAATCCAAAATTGCTATATTTGGTAAATATTTTAGTTTATTTTACTATATCTGAAAATGTTTCTTAATCTTAAATACGAAACTTAATTAATAATAGTTTCTTAGATAAATTATTATTCATTAACACTGAAGATTAGAAAAAAAAAAAAGTATTTAGTGGAAAATCAAAATTAGAAGTGTAAATCTTGCCGCCTAAGACCAAATTGAAATAAATTCAAATGTGAAGCCTAAATTGAACCAACTCTTAAGATAATAACTAACTATATATATATATATATGTTTTTCCCCATAATTTGTTTCCTCATTTTTTTAATCACAATATTTATAATATACACCAAACTTTGTAAAATATTATTACAACTCTATATTAAACAATTATCACAGTAAATAGTTGAGATCGTGAATTTATTCCATAAAAAAATTACAAATTGTATTGCCCCTTAGAGTTGATATGATGCAGAGGAATAGACGCACCAGAAAGTGCAATGCCTTATGGGAAAGAGGCAAAAGAAGAACTGAAAAGGCTTGTTGAAGGCAAGTGTTTGAGAGTACATGTGTATGGTGAAGATCGGTACAATCGATGTGTGGGTGACTTGTATTGTAATGGAAAGTTTATTCAGGTAAAACACAAATTTACACTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTTCGCTATGGTATAGTCTTCATGTTCATGTTATTGGTTTAGACAAATACAAGTTTTTGGTTGTCTTTACAGGAAGCAATGCTGAAAAAGGGGTTTGCTTGGCATTACACAGCATATGACAAACGTCCAGAGCTGTCAAAGGCAAGGGCTAAATTTACCATTTAA

mRNA sequence

GGCATGTCAAACTCACACTTAATCAAGTTTTGTCTCATGGGCAACAGGGACTATTGACATTCTATGGTCTTCCTCTTCCTCATACTCTCGTCAAACCTTCTGCTCCGGTTCCAACTGCCGTCTCGATACCTGACGGAGTCAAGTTTGAGCTGCAGACGCTGCCAGTTGATGCCAAAGCTGTAGCAGATGGTGATACAGTGACAGTGTATGTGAGCACATCACACCCGAGAGAGTCCTCTTGTGTTCCAAAAGAGGTTAGAAATGCAGCCCTTCAAAGATCACGAGCTAGAAACGCAAAGAACTTTGCAAAGGCCGATGCTCTTCACAAGACAATCACGGATGCTGGTTACAGAGGAATAGACGCACCAGAAAGTGCAATGCCTTATGGGAAAGAGGCAAAAGAAGAACTGAAAAGGCTTGTTGAAGGCAAGTGTTTGAGAGTACATGTGTATGGTGAAGATCGGTACAATCGATGTGTGGGTGACTTGTATTGTAATGGAAAGTTTATTCAGGAAGCAATGCTGAAAAAGGGGTTTGCTTGGCATTACACAGCATATGACAAACGTCCAGAGCTGTCAAAGGCAAGGGCTAAATTTACCATTTAA

Coding sequence (CDS)

GGCATGTCAAACTCACACTTAATCAAGTTTTGTCTCATGGGCAACAGGGACTATTGACATTCTATGGTCTTCCTCTTCCTCATACTCTCGTCAAACCTTCTGCTCCGGTTCCAACTGCCGTCTCGATACCTGACGGAGTCAAGTTTGAGCTGCAGACGCTGCCAGTTGATGCCAAAGCTGTAGCAGATGGTGATACAGTGACAGTGTATGTGAGCACATCACACCCGAGAGAGTCCTCTTGTGTTCCAAAAGAGGTTAGAAATGCAGCCCTTCAAAGATCACGAGCTAGAAACGCAAAGAACTTTGCAAAGGCCGATGCTCTTCACAAGACAATCACGGATGCTGGTTACAGAGGAATAGACGCACCAGAAAGTGCAATGCCTTATGGGAAAGAGGCAAAAGAAGAACTGAAAAGGCTTGTTGAAGGCAAGTGTTTGAGAGTACATGTGTATGGTGAAGATCGGTACAATCGATGTGTGGGTGACTTGTATTGTAATGGAAAGTTTATTCAGGAAGCAATGCTGAAAAAGGGGTTTGCTTGGCATTACACAGCATATGACAAACGTCCAGAGCTGTCAAAGGCAAGGGCTAAATTTACCATTTAA

Protein sequence

HVKLTLNQVLSHGQQGLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGYRGIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPELSKARAKFTI*
Homology
[Close]
BLAST of Cucsa.144210 vs. Swiss-Prot
Match: CAN1_ARATH (Staphylococcal-like nuclease CAN1 OS=Arabidopsis thaliana GN=CAN1 PE=1 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 3.5e-63
Identity = 121/198 (61.11%), Postives = 145/198 (73.23%), Query Frame = 1 

Query: 15  QGLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSH 74
           +GLL+FYGLP PH LV+   P    VS+P GV+FEL TLPVD K+VADGDTVTVYVS+  
Sbjct: 96  EGLLSFYGLPSPHNLVE--VPTEAPVSLPKGVRFELNTLPVDTKSVADGDTVTVYVSSKD 155

Query: 75  PRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGYR----------------- 134
           P  SS +PK+V  AA++R++AR  KN+ +ADALHKTI  +GYR                 
Sbjct: 156 PLVSSSLPKDVSLAAVKRAKAREKKNYTEADALHKTIIASGYRMISFQNEEVLAKKFRIR 215

Query: 135 --GIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLK 194
             GID+PES MPYGKEA +EL ++VEGKCL+V VY EDRY RCVGD+YCNGKF+QE MLK
Sbjct: 216 LSGIDSPESKMPYGKEAHDELLKMVEGKCLKVLVYTEDRYGRCVGDIYCNGKFVQEVMLK 275

[Close]
BLAST of Cucsa.144210 vs. Swiss-Prot
Match: CAN1_ORYSJ (Probable staphylococcal-like nuclease CAN1 OS=Oryza sativa subsp. japonica GN=Os01g0166100 PE=2 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 1.8e-62
Identity = 118/199 (59.30%), Postives = 142/199 (71.36%), Query Frame = 1 

Query: 15  QGLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSH 74
           +G L FYGLP+P+      AP    V  P G KFEL TLPVDAKAVADGDT+TVY+ T+ 
Sbjct: 100 EGFLVFYGLPIPNAAASTPAPHTAHVPKPQGCKFELHTLPVDAKAVADGDTITVYIDTAD 159

Query: 75  PRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGY------------------ 134
           PRES  VP+E++ AA +R+RAR A+++ KAD L K I DAGY                  
Sbjct: 160 PRESGNVPREIQKAAAERTRARAARDYQKADGLQKMIADAGYRQVPNARGEEVLAKKYRI 219

Query: 135 --RGIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAML 194
             RGIDAPESAMPYGKEAKE L ++V+GK L+V+VY EDRY RCVGD+YC+G F+QE ML
Sbjct: 220 RLRGIDAPESAMPYGKEAKEALLKMVQGKSLKVYVYDEDRYGRCVGDIYCDGVFVQEQML 279

[Close]
BLAST of Cucsa.144210 vs. Swiss-Prot
Match: Y38K_CAPSE (Uncharacterized 38.1 kDa protein OS=Capnoides sempervirens PE=2 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 1.5e-61
Identity = 127/217 (58.53%), Postives = 149/217 (68.66%), Query Frame = 1 

Query: 4   LTLNQVLSHGQQGLLTFYGLPLPHTLVKPSAPVPTAVSIPD---GVKFELQTLPVDAKAV 63
           LTL +      +GLL FYGLPL +    PS    T V+ P    GVKFEL TLPVD KAV
Sbjct: 97  LTLKRHQKADVKGLLRFYGLPLSNN---PSTEATTVVAPPQADQGVKFELHTLPVDVKAV 156

Query: 64  ADGDTVTVYVSTSHPRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGY---- 123
           ADGDTVTVYV+T  PRE+S +PK V+ AA +R++AR  +++ KADAL K I DAGY    
Sbjct: 157 ADGDTVTVYVNTEDPREASNLPKSVKVAAQERAKARAVRDYVKADALQKNIVDAGYRVLS 216

Query: 124 ----------------RGIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVG 183
                           RGID+PES+MP+GKEAKEEL +LV GKCL VH+Y EDRY R VG
Sbjct: 217 GPNNEDILARKYRIRLRGIDSPESSMPFGKEAKEELIKLVVGKCLTVHIYEEDRYGRSVG 276

Query: 184 DLYCNGKFIQEAMLKKGFAWHYTAYDKRPELSKARAK 198
           D+YCNG+FIQE MLKKG AWHYTAYDKRP+LSK   K
Sbjct: 277 DIYCNGQFIQEKMLKKGLAWHYTAYDKRPQLSKWEEK 310

[Close]
BLAST of Cucsa.144210 vs. Swiss-Prot
Match: CAN2_ARATH (Staphylococcal-like nuclease CAN2 OS=Arabidopsis thaliana GN=CAN2 PE=1 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 1.5e-61
Identity = 121/198 (61.11%), Postives = 145/198 (73.23%), Query Frame = 1 

Query: 15  QGLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSH 74
           +GLL+FYGLPL HTLV+ +   P  VS+P+G+ FE QTLPVD KAVADGDT+TVYVSTS 
Sbjct: 106 EGLLSFYGLPLSHTLVEVTVEAP--VSLPEGILFEFQTLPVDPKAVADGDTITVYVSTSE 165

Query: 75  PRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGYR----------------- 134
           P  SS VP+EV  AA+QR++AR  +N+ KAD LH+ I D+GYR                 
Sbjct: 166 PVVSSSVPREVNLAAVQRAKAREKRNYPKADELHQKIIDSGYRVLNIENEEVLARKFRIR 225

Query: 135 --GIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLK 194
             GIDAPES MP+GKEA+E L ++V  K L+V VYGED+Y RCVGDLYCNG F+QEAMLK
Sbjct: 226 LRGIDAPESQMPFGKEAQEGLLKIVGRKSLKVLVYGEDQYGRCVGDLYCNGIFVQEAMLK 285

[Close]
BLAST of Cucsa.144210 vs. Swiss-Prot
Match: CAN3_ORYSJ (Probable staphylococcal-like nuclease CAN3 OS=Oryza sativa subsp. japonica GN=Os12g0109200 PE=2 SV=2)

HSP 1 Score: 175.6 bits (444), Expect = 5.3e-43
Identity = 92/211 (43.60%), Postives = 128/211 (60.66%), Query Frame = 1 

Query: 2   VKLTLNQVLSHGQQGLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVA 61
           +++T N V     QG+L FY  P+P      S   P+  S+P+GV+F L TLPV  K + 
Sbjct: 133 LEITSNYV---SPQGILEFYNFPIPSLPSASSNYQPS--SLPEGVQFVLNTLPVYDKCIG 192

Query: 62  DGDTVTVYVSTSHPRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGY----- 121
           DGD  T YVST+ PRES+ VP EV    + R++AR  +++  ADAL  ++ +AGY     
Sbjct: 193 DGDGFTAYVSTTDPRESANVPLEVHELVIARTQARKCRDYQSADALLSSLDEAGYKIISC 252

Query: 122 --------------RGIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDL 181
                         RGIDAPE  MPYG+E++  L +L+ GK ++++VY  D++ R VGD+
Sbjct: 253 SDDEVLARKYRIRMRGIDAPELKMPYGRESRNALVKLIGGKSVKIYVYDLDQFGRYVGDI 312

Query: 182 YCNGKFIQEAMLKKGFAWHYTAYDKRPELSK 194
           YCN  FIQE MLK G AWH+  YDKRPE ++
Sbjct: 313 YCNNLFIQEQMLKNGHAWHFKTYDKRPEFAR 338

[Close]
BLAST of Cucsa.144210 vs. TrEMBL
Match: B6CAZ6_CUCSA (Calcium-dependent nuclease OS=Cucumis sativus GN=Csa_5G218750 PE=2 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 1.4e-95
Identity = 184/216 (85.19%), Postives = 186/216 (86.11%), Query Frame = 1 

Query: 5   TLNQVLSHGQQGLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGD 64
           TL +      +GLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGD
Sbjct: 95  TLKRHQKKDVEGLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGD 154

Query: 65  TVTVYVSTSHPRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGY-------- 124
           TVTVYVSTSHPRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGY        
Sbjct: 155 TVTVYVSTSHPRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGYRVITFQNE 214

Query: 125 -----------RGIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCN 184
                      RGIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCN
Sbjct: 215 EVLAKKYRIRLRGIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCN 274

Query: 185 GKFIQEAMLKKGFAWHYTAYDKRPELSK----ARAK 198
           GKFIQEAMLKKGFAWHYTAYDKRPELSK    ARAK
Sbjct: 275 GKFIQEAMLKKGFAWHYTAYDKRPELSKWENEARAK 310

[Close]
BLAST of Cucsa.144210 vs. TrEMBL
Match: A0A061FJY4_THECC (Uncharacterized protein isoform 1 OS=Theobroma cacao GN=TCM_042417 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.0e-69
Identity = 138/206 (66.99%), Postives = 160/206 (77.67%), Query Frame = 1 

Query: 15  QGLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSH 74
           +GLL FYGLPLPH LV+PSA  PT  S+P GVKFELQTLPVD KA+ DGDT+TVYVST+ 
Sbjct: 108 EGLLAFYGLPLPHALVQPSASAPT--SLPQGVKFELQTLPVDVKAIPDGDTITVYVSTTD 167

Query: 75  PRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGY------------------ 134
           PRES+ VP++V+ AA+QRS AR  KN+ +ADALHK ITDAGY                  
Sbjct: 168 PRESANVPRDVQLAAVQRSEARAEKNYTEADALHKKITDAGYRVLNLQNQEILARKYRIR 227

Query: 135 -RGIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLK 194
            RGIDAPES+MPYGKEAKEEL +LV+GKCLRV VYGEDRY RCVGD+YCNGKF+QE MLK
Sbjct: 228 LRGIDAPESSMPYGKEAKEELVKLVQGKCLRVLVYGEDRYGRCVGDIYCNGKFVQEIMLK 287

Query: 195 KGFAWHYTAYDKRPELS----KARAK 198
           KG AWHY+AYD+R EL+    +ARAK
Sbjct: 288 KGLAWHYSAYDQRIELATWEKEARAK 311

[Close]
BLAST of Cucsa.144210 vs. TrEMBL
Match: A0A061FKQ6_THECC (Nuclease isoform 2 OS=Theobroma cacao GN=TCM_042417 PE=4 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 3.0e-69
Identity = 134/197 (68.02%), Postives = 155/197 (78.68%), Query Frame = 1 

Query: 15  QGLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSH 74
           +GLL FYGLPLPH LV+PSA  PT  S+P GVKFELQTLPVD KA+ DGDT+TVYVST+ 
Sbjct: 108 EGLLAFYGLPLPHALVQPSASAPT--SLPQGVKFELQTLPVDVKAIPDGDTITVYVSTTD 167

Query: 75  PRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGY------------------ 134
           PRES+ VP++V+ AA+QRS AR  KN+ +ADALHK ITDAGY                  
Sbjct: 168 PRESANVPRDVQLAAVQRSEARAEKNYTEADALHKKITDAGYRVLNLQNQEILARKYRIR 227

Query: 135 -RGIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLK 193
            RGIDAPES+MPYGKEAKEEL +LV+GKCLRV VYGEDRY RCVGD+YCNGKF+QE MLK
Sbjct: 228 LRGIDAPESSMPYGKEAKEELVKLVQGKCLRVLVYGEDRYGRCVGDIYCNGKFVQEIMLK 287

[Close]
BLAST of Cucsa.144210 vs. TrEMBL
Match: B9HHW7_POPTR (Ca(2+)-dependent nuclease family protein OS=Populus trichocarpa GN=POPTR_0008s07470g PE=4 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 4.4e-68
Identity = 134/206 (65.05%), Postives = 157/206 (76.21%), Query Frame = 1 

Query: 15  QGLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSH 74
           +GLL FYGLPLPHTL++ S  +P   S+P+GVKFE+ TLPVDAKAVADGDT+TVYVST+ 
Sbjct: 108 EGLLAFYGLPLPHTLIQVSTGIPA--SLPEGVKFEMPTLPVDAKAVADGDTITVYVSTTD 167

Query: 75  PRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGY------------------ 134
           PRES+ VP+EV+ A +QRS+AR  +N+ KADALHK I +AGY                  
Sbjct: 168 PRESAYVPREVQTATVQRSKARAERNYGKADALHKKIVEAGYRVLNIQNQEILARKYRIR 227

Query: 135 -RGIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLK 194
            RGIDAPESAMPYGKEAKEEL  LV+GKCLR+ VYGEDRY RCVGD+YCNG F+QE MLK
Sbjct: 228 LRGIDAPESAMPYGKEAKEELANLVQGKCLRIFVYGEDRYGRCVGDIYCNGIFVQEIMLK 287

Query: 195 KGFAWHYTAYDKRPEL----SKARAK 198
           KG AWHYTAYD+R EL     +ARAK
Sbjct: 288 KGLAWHYTAYDQRWELETWEKEARAK 311

[Close]
BLAST of Cucsa.144210 vs. TrEMBL
Match: A1KYZ0_POPTO (Ca(2+)-dependent DNase OS=Populus tomentosa GN=CDD PE=2 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 1.7e-67
Identity = 134/206 (65.05%), Postives = 156/206 (75.73%), Query Frame = 1 

Query: 15  QGLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSH 74
           +GLL FYGLPL HTLV+ S  +PT  S+P+GVKFE+ TLPVDAKAVADGDT+TVYVST+ 
Sbjct: 108 EGLLAFYGLPLAHTLVQVSTGIPT--SLPEGVKFEMPTLPVDAKAVADGDTITVYVSTTD 167

Query: 75  PRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGY------------------ 134
           PRES+ VP+EV+ A +QRS+AR  +N+ KADALHK I +AGY                  
Sbjct: 168 PRESAYVPREVQTATVQRSKARAERNYGKADALHKKIVEAGYRVLNIQNQEILARKYRIR 227

Query: 135 -RGIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLK 194
            RGIDAPESAMPYGKEAKEE   LV+GKCLR+ VYGEDRY RCVGD+YCNG F+QE MLK
Sbjct: 228 LRGIDAPESAMPYGKEAKEEFANLVQGKCLRIFVYGEDRYGRCVGDIYCNGIFVQEVMLK 287

Query: 195 KGFAWHYTAYDKRPEL----SKARAK 198
           KG AWHYTAYD+R EL     +ARAK
Sbjct: 288 KGLAWHYTAYDQRWELETWEKEARAK 311

[Close]
BLAST of Cucsa.144210 vs. TAIR10
Match: AT3G56170.1 (AT3G56170.1 Ca-2+ dependent nuclease)

HSP 1 Score: 242.7 bits (618), Expect = 2.0e-64
Identity = 121/198 (61.11%), Postives = 145/198 (73.23%), Query Frame = 1 

Query: 15  QGLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSH 74
           +GLL+FYGLP PH LV+   P    VS+P GV+FEL TLPVD K+VADGDTVTVYVS+  
Sbjct: 96  EGLLSFYGLPSPHNLVE--VPTEAPVSLPKGVRFELNTLPVDTKSVADGDTVTVYVSSKD 155

Query: 75  PRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGYR----------------- 134
           P  SS +PK+V  AA++R++AR  KN+ +ADALHKTI  +GYR                 
Sbjct: 156 PLVSSSLPKDVSLAAVKRAKAREKKNYTEADALHKTIIASGYRMISFQNEEVLAKKFRIR 215

Query: 135 --GIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLK 194
             GID+PES MPYGKEA +EL ++VEGKCL+V VY EDRY RCVGD+YCNGKF+QE MLK
Sbjct: 216 LSGIDSPESKMPYGKEAHDELLKMVEGKCLKVLVYTEDRYGRCVGDIYCNGKFVQEVMLK 275

[Close]
BLAST of Cucsa.144210 vs. TAIR10
Match: AT2G40410.2 (AT2G40410.2 Staphylococcal nuclease homologue)

HSP 1 Score: 237.3 bits (604), Expect = 8.4e-63
Identity = 121/198 (61.11%), Postives = 145/198 (73.23%), Query Frame = 1 

Query: 15  QGLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSH 74
           +GLL+FYGLPL HTLV+ +   P  VS+P+G+ FE QTLPVD KAVADGDT+TVYVSTS 
Sbjct: 106 EGLLSFYGLPLSHTLVEVTVEAP--VSLPEGILFEFQTLPVDPKAVADGDTITVYVSTSE 165

Query: 75  PRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGYR----------------- 134
           P  SS VP+EV  AA+QR++AR  +N+ KAD LH+ I D+GYR                 
Sbjct: 166 PVVSSSVPREVNLAAVQRAKAREKRNYPKADELHQKIIDSGYRVLNIENEEVLARKFRIR 225

Query: 135 --GIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLK 194
             GIDAPES MP+GKEA+E L ++V  K L+V VYGED+Y RCVGDLYCNG F+QEAMLK
Sbjct: 226 LRGIDAPESQMPFGKEAQEGLLKIVGRKSLKVLVYGEDQYGRCVGDLYCNGIFVQEAMLK 285

[Close]
BLAST of Cucsa.144210 vs. NCBI nr
Match: gi|568384727|ref|NP_001275526.1| (uncharacterized protein LOC101213685 [Cucumis sativus])

HSP 1 Score: 357.1 bits (915), Expect = 2.1e-95
Identity = 184/216 (85.19%), Postives = 186/216 (86.11%), Query Frame = 1 

Query: 5   TLNQVLSHGQQGLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGD 64
           TL +      +GLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGD
Sbjct: 95  TLKRHQKKDVEGLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGD 154

Query: 65  TVTVYVSTSHPRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGY-------- 124
           TVTVYVSTSHPRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGY        
Sbjct: 155 TVTVYVSTSHPRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGYRVITFQNE 214

Query: 125 -----------RGIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCN 184
                      RGIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCN
Sbjct: 215 EVLAKKYRIRLRGIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCN 274

Query: 185 GKFIQEAMLKKGFAWHYTAYDKRPELSK----ARAK 198
           GKFIQEAMLKKGFAWHYTAYDKRPELSK    ARAK
Sbjct: 275 GKFIQEAMLKKGFAWHYTAYDKRPELSKWENEARAK 310

[Close]
BLAST of Cucsa.144210 vs. NCBI nr
Match: gi|659114095|ref|XP_008456902.1| (PREDICTED: uncharacterized 38.1 kDa protein [Cucumis melo])

HSP 1 Score: 343.6 bits (880), Expect = 2.4e-91
Identity = 178/216 (82.41%), Postives = 182/216 (84.26%), Query Frame = 1 

Query: 5   TLNQVLSHGQQGLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGD 64
           TL +      +GLLTFYGLPLPHTLVK SAP PTA SIPDGVKFELQTLPVDAKAVADGD
Sbjct: 95  TLKRHQKKDVEGLLTFYGLPLPHTLVKSSAPAPTAASIPDGVKFELQTLPVDAKAVADGD 154

Query: 65  TVTVYVSTSHPRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGY-------- 124
           TVTV+VSTSHPRESSCVPKEVRNAALQRSRARNAKN+AKADALHKTITDAGY        
Sbjct: 155 TVTVHVSTSHPRESSCVPKEVRNAALQRSRARNAKNYAKADALHKTITDAGYRVITFQNE 214

Query: 125 -----------RGIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCN 184
                      RGIDAPESAMPYGKEAKEELKRLVEGKCLRV VYGEDRYNRCVGDLYCN
Sbjct: 215 EVLAKKYRIRLRGIDAPESAMPYGKEAKEELKRLVEGKCLRVLVYGEDRYNRCVGDLYCN 274

Query: 185 GKFIQEAMLKKGFAWHYTAYDKRPELSK----ARAK 198
           GKFIQEAMLKKGFAWHYTAYDKRPELSK    ARAK
Sbjct: 275 GKFIQEAMLKKGFAWHYTAYDKRPELSKWENEARAK 310

[Close]
BLAST of Cucsa.144210 vs. NCBI nr
Match: gi|778697890|ref|XP_011654429.1| (PREDICTED: uncharacterized protein LOC101213685 isoform X1 [Cucumis sativus])

HSP 1 Score: 278.1 bits (710), Expect = 1.2e-71
Identity = 148/193 (76.68%), Postives = 153/193 (79.27%), Query Frame = 1 

Query: 31  KPSAPVPTAVS---IPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRN 90
           KP+   P   S   I    + + + + VDAKAVADGDTVTVYVSTSHPRESSCVPKEVRN
Sbjct: 79  KPTPRTPEEASRLVIQTLKRHQKKDVEVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRN 138

Query: 91  AALQRSRARNAKNFAKADALHKTITDAGY-------------------RGIDAPESAMPY 150
           AALQRSRARNAKNFAKADALHKTITDAGY                   RGIDAPESAMPY
Sbjct: 139 AALQRSRARNAKNFAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPY 198

Query: 151 GKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 198
           GKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR
Sbjct: 199 GKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 258

[Close]
BLAST of Cucsa.144210 vs. NCBI nr
Match: gi|590561526|ref|XP_007008835.1| (Uncharacterized protein isoform 1 [Theobroma cacao])

HSP 1 Score: 271.2 bits (692), Expect = 1.5e-69
Identity = 138/206 (66.99%), Postives = 160/206 (77.67%), Query Frame = 1 

Query: 15  QGLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSH 74
           +GLL FYGLPLPH LV+PSA  PT  S+P GVKFELQTLPVD KA+ DGDT+TVYVST+ 
Sbjct: 108 EGLLAFYGLPLPHALVQPSASAPT--SLPQGVKFELQTLPVDVKAIPDGDTITVYVSTTD 167

Query: 75  PRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGY------------------ 134
           PRES+ VP++V+ AA+QRS AR  KN+ +ADALHK ITDAGY                  
Sbjct: 168 PRESANVPRDVQLAAVQRSEARAEKNYTEADALHKKITDAGYRVLNLQNQEILARKYRIR 227

Query: 135 -RGIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLK 194
            RGIDAPES+MPYGKEAKEEL +LV+GKCLRV VYGEDRY RCVGD+YCNGKF+QE MLK
Sbjct: 228 LRGIDAPESSMPYGKEAKEELVKLVQGKCLRVLVYGEDRYGRCVGDIYCNGKFVQEIMLK 287

Query: 195 KGFAWHYTAYDKRPELS----KARAK 198
           KG AWHY+AYD+R EL+    +ARAK
Sbjct: 288 KGLAWHYSAYDQRIELATWEKEARAK 311

[Close]
BLAST of Cucsa.144210 vs. NCBI nr
Match: gi|743781855|ref|XP_011005462.1| (PREDICTED: staphylococcal-like nuclease CAN2 [Populus euphratica])

HSP 1 Score: 269.6 bits (688), Expect = 4.3e-69
Identity = 135/206 (65.53%), Postives = 159/206 (77.18%), Query Frame = 1 

Query: 15  QGLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSH 74
           +GLL FYGLPLPHTLV+  A +PT  S+P+GVKFE+ TLPVDA+AVADGDT+TVYVST+ 
Sbjct: 108 EGLLAFYGLPLPHTLVQVCAGIPT--SLPEGVKFEMPTLPVDARAVADGDTITVYVSTTE 167

Query: 75  PRESSCVPKEVRNAALQRSRARNAKNFAKADALHKTITDAGYR----------------- 134
           PRES+CVP+EV+ A +QRS+AR  +N+ KADALHKTI +AGYR                 
Sbjct: 168 PRESACVPREVQKATVQRSKARAERNYGKADALHKTIIEAGYRVINIQNKEILARKYRIR 227

Query: 135 --GIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLK 194
             GIDAPES MPYG+EAKEEL  LV+GKCLR+ VYGEDRY RCVGD+YCNG F+QE MLK
Sbjct: 228 LRGIDAPESEMPYGQEAKEELANLVQGKCLRIFVYGEDRYGRCVGDIYCNGIFVQEIMLK 287

Query: 195 KGFAWHYTAYDKRPEL----SKARAK 198
           KG AWHYTAYD+R EL     +ARAK
Sbjct: 288 KGLAWHYTAYDQRWELETWEKEARAK 311

The following BLAST results are available for this feature:
BLAST of Cucsa.144210 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CAN1_ARATH3.5e-6361.11Staphylococcal-like nuclease CAN1 OS=Arabidopsis thaliana GN=CAN1 PE=1 SV=1[more]
CAN1_ORYSJ1.8e-6259.30Probable staphylococcal-like nuclease CAN1 OS=Oryza sativa subsp. japonica GN=Os... [more]
Y38K_CAPSE1.5e-6158.53Uncharacterized 38.1 kDa protein OS=Capnoides sempervirens PE=2 SV=1[more]
CAN2_ARATH1.5e-6161.11Staphylococcal-like nuclease CAN2 OS=Arabidopsis thaliana GN=CAN2 PE=1 SV=1[more]
CAN3_ORYSJ5.3e-4343.60Probable staphylococcal-like nuclease CAN3 OS=Oryza sativa subsp. japonica GN=Os... [more]
BLAST of Cucsa.144210 vs. TrEMBL
Match NameE-valueIdentityDescription
B6CAZ6_CUCSA1.4e-9585.19Calcium-dependent nuclease OS=Cucumis sativus GN=Csa_5G218750 PE=2 SV=1[more]
A0A061FJY4_THECC1.0e-6966.99Uncharacterized protein isoform 1 OS=Theobroma cacao GN=TCM_042417 PE=4 SV=1[more]
A0A061FKQ6_THECC3.0e-6968.02Nuclease isoform 2 OS=Theobroma cacao GN=TCM_042417 PE=4 SV=1[more]
B9HHW7_POPTR4.4e-6865.05Ca(2+)-dependent nuclease family protein OS=Populus trichocarpa GN=POPTR_0008s07... [more]
A1KYZ0_POPTO1.7e-6765.05Ca(2+)-dependent DNase OS=Populus tomentosa GN=CDD PE=2 SV=1[more]
BLAST of Cucsa.144210 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G56170.12.0e-6461.11 Ca-2+ dependent nuclease[more]
AT2G40410.28.4e-6361.11 Staphylococcal nuclease homologue[more]
BLAST of Cucsa.144210 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|568384727|ref|NP_001275526.1|2.1e-9585.19uncharacterized protein LOC101213685 [Cucumis sativus][more]
gi|659114095|ref|XP_008456902.1|2.4e-9182.41PREDICTED: uncharacterized 38.1 kDa protein [Cucumis melo][more]
gi|778697890|ref|XP_011654429.1|1.2e-7176.68PREDICTED: uncharacterized protein LOC101213685 isoform X1 [Cucumis sativus][more]
gi|590561526|ref|XP_007008835.1|1.5e-6966.99Uncharacterized protein isoform 1 [Theobroma cacao][more]
gi|743781855|ref|XP_011005462.1|4.3e-6965.53PREDICTED: staphylococcal-like nuclease CAN2 [Populus euphratica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR009080tRNAsynth_Ia_anticodon-bd
IPR016071Staphylococal_nuclease_OB-fold
Vocabulary: Molecular Function
TermDefinition
GO:0000166nucleotide binding
GO:0004812aminoacyl-tRNA ligase activity
GO:0005524ATP binding
Vocabulary: Biological Process
TermDefinition
GO:0006418tRNA aminoacylation for protein translation
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006418 tRNA aminoacylation for protein translation
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.144210.1Cucsa.144210.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009080Aminoacyl-tRNA synthetase, class Ia, anticodon-bindingunknownSSF47323Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetasescoord: 75..123
score: 8.7
IPR016071Staphylococcal nuclease (SNase-like), OB-foldGENE3DG3DSA:2.40.50.90coord: 110..180
score: 1.2E-29coord: 48..72
score: 1.2
IPR016071Staphylococcal nuclease (SNase-like), OB-foldPFAMPF00565SNasecoord: 117..192
score: 6.3
IPR016071Staphylococcal nuclease (SNase-like), OB-foldSMARTSM00318SNASE_2coord: 51..197
score: 1.5
IPR016071Staphylococcal nuclease (SNase-like), OB-foldPROFILEPS50830TNASE_3coord: 51..200
score: 21
IPR016071Staphylococcal nuclease (SNase-like), OB-foldunknownSSF50199Staphylococcal nucleasecoord: 107..191
score: 5.89E-23coord: 52..69
score: 5.89
NoneNo IPR availablePANTHERPTHR12302EBNA2 BINDING PROTEIN P100coord: 14..22
score: 8.4E-53coord: 39..194
score: 8.4