Carg24489 (gene) Silver-seed gourd

NameCarg24489
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptionstaphylococcal-like nuclease CAN1
LocationCucurbita_argyrosperma_scaffold_303 : 136195 .. 139191 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTCATTACCTTAGATTCGCCATTGTTTAAGGCAGCAAAAAATGGGGAACGTTCTCAGGTTCCTCTGCGGTCACTTCTGCAATCCTAACGCTGCCGCCGACGCCGATTCCCTCGGTCCTCATGGCGTCTCGCCGGCCACCGTCGGCGTCTCGGCTCTGGCTCACGATCTCTTTGATTTCGAGATCACCTCGCAGGTATGTTCTCAATCTGAAGCTCATCTCGAGCTGCATTTCTGGTTGATTGAATTTTGAATCGGATTCTGTCTGGTGATCTAGTGAAGTTTGTTGTTCAAACGATCTATGCTTTGGTATTGATGATTTTGATTTGATGAATTTTTGAAAGGTTCCTCAAGGACTTAGTAAGCATGTGGTTTCGTCGAAGAAGGCACAGGCGAATTGGTAAAGTATTTCTGATTGTTTGGTTACAGAGTGGAGATTTGTGAAAGTAATTGTAGTTACAGAGTGGAGAGATTCTGATTGTTTGTGATGATCTGTGTGTGTGTGTGAAGGTATAGAAAACTGGCTGAAGCTTGGAGGTCGGAGAAACCACCTCCTAAAACGCCTGAAGAAGCATCCAGGCTTGTGATTCAGACACTCAAGAGACACCAGAAGAAAGATGTCGAGGTACAAATAGGAATTCTCTTGCTTGTTTTGTTGGATTGGTAAACTGGGAATCTCTGCAGGTTAAATAGTTCAATTGATAAGAGTCAAAGTTCATTCTGTCAAATATGAGCAGCTCCGATGATAATAAGGAAATGCGTTGTTCTTTCCATTTCGAATTCAAATTTTTAGGAGAGAGAAATCAGATCCATGGGGACTTAGCTAGATGGCTTGTGTAGAACAGTCCAAAATCCAGTGGGGTTGGATCTGATGAACTGAAATGGAAAACAGATTACTTTTTGAAAAGTGTCAAATTACTTTTTGAAAATAGTGCTTTCAAGAACGTTTATGTTTTTAAAAAATACTTAAATGCTTCCTTTAAAAGCATTTAAGTACTTTCTTTTCATTTTTAAAACTTCTTAACCACTTTTGTTCTTGCAAAATCAAGCTATAAGTTAAAAAAAAAAAAAAAAAAAAAATACAACAACAATTTTAGTCCTCCATTAGCATTTTAAAAGGAATGTCAAACACACTCAATCAAGTTCTTTGTTTTTATTTCTGTTGTTCAACAGGGATTATTGGCTTTCTATGGTCTACCTCTTCCTCATACTCTTGTCGAACTTTCTGCTCCGGCCCCAGTGCCTACATCGCTACCAGATGGAGTCAAGTTTGAGCTGCAGACATTGCCAGTAAGTGAGAAAGGATGCTTTTATGGGAAATGACCAACCATTTTGTTGATAACAGGTGTGTTCTCATTTCTAAATGTTGAATTCATACCAGGTTGATGCAAAAGCTGTAGCAGATGGTGATACAGTGACAGTGTATGTGAGTACAGCACACCCTCGAGAGTCCTCTTGTATTCCAATGGAGGTTAGGAACGCAGCCATTCAAAGATCACAAGCTAGAAACGCCAAGAACTACGCAAAGGCTGATGCACTTCACAAGACAATAACTGATGCTGGTTACAGGTAAATTATATCTCTTCTCTACTGCATACATACACTTGGACTTTTTCAATAAGTTATTGGGATTTGATGCCTGTTTCTTCTCCCTCAAAAGGGTGCTTACTGTTCAGAATGAGGAGATTCTTGCAAGAAAATACAGGATTCGTCTCAGGTAATTAAAATTAGCACCGTGTGATTTGATTCTTGAACTTTCAGTTTTTTTGTTTTTCATTTTGATGTATACTTTTTCAAATCGTTTGTCTATTTTGATTCATCTAATTTCAAAATGTCTATTTTAGTCTTTATACTATTTAGAAGTTCACACATTTATATTTTCTCATATTTTTTTTTCATCTCAATTTCATAGCAAAACACTACATTCCAAATTTACTAGAAATACTCTCTTTTAACTTTTATTAGATGATGATTATTGTGCATAGAATCCAGGATTTGCAAATAGACTAAATATTCAGTTTTCAAGAGCACAAAATCAAAATACGATTTAAACCTGAATAAAAATAGTGATTGTTCCATACTTGAACGATTTCCAAACTCCATTGACCCTTAGGCTTAGTTGAACTTTGATAATGCAGAGGAATAGATGCACCAGAAAGCGCAATGCCTTATGGGAAAGAGGCAAAAGAAGAGCTGAGAAAGCTTGTTGAAGGCAAGTGTTTGAGAGTACTTGTGTACGGAGAAGATCGGTATAACCGATGTGTGGGTGACTTGTATTGTAATGGAAAGTTTATTCAGGTAACACTAATTACACTATTTCCACTTTCCTCGATGGATCATATGGCTTCATGTTTCTGGTTATGTAAGAATAAGTTTTTGGTTCTCTCTACAGGAAGCAATGCTGAAAAAGGGCTTTGCGTGGCATTACACTGCCTATGACAAACGAGCAGAGCTGGCAAAGGTGCGTTACATTTTGATTTCATTCGTTAAACTTATATCCCATAAACTTCATGTAACACTTTTGACCCTTGAGATTTAAATTAAACTTCTGTTTGGTCCCTAAAGTTTGAACTAGATTTGTAACTAGTTCTGAAATTTGAAATGCTAACGTGATAGTTAAATATACTTAACTTGCCTTTGCCTTTGATGTATGTATGTTTTAAATGAATTAAATATATATTATGTTTTATAAACGTACCAATTATAAATCAATTTCACATCCACCTCAACATTTTGGAAAAAAGAACTACAATTCAGACAGATATTGTCACATTCAAGTGTACAAAGAGTTTTAACAGAGGGAAAGTGTTACGTGTATGCTACTTTACATGATCACTTTACTCAATTCTAAATTTCTAATGGATTCTAATTCATGATTCATAACAGTGGGAAAATGAAGCTCGAGCTAAAAGGATAGGGTTGTGGGCTTCTAGAAACCCAGAGCAGCCATGGGAATGGAGAAAGGGAAAGCGTGAAGGGAAATAGTATACACAATTTA

mRNA sequence

ATTTCATTACCTTAGATTCGCCATTGTTTAAGGCAGCAAAAAATGGGGAACGTTCTCAGGTTCCTCTGCGGTCACTTCTGCAATCCTAACGCTGCCGCCGACGCCGATTCCCTCGGTCCTCATGGCGTCTCGCCGGCCACCGTCGGCGTCTCGGCTCTGGCTCACGATCTCTTTGATTTCGAGATCACCTCGCAGGTTCCTCAAGGACTTAGTAAGCATGTGGTTTCGTCGAAGAAGGCACAGGCGAATTGGTATAGAAAACTGGCTGAAGCTTGGAGGTCGGAGAAACCACCTCCTAAAACGCCTGAAGAAGCATCCAGGCTTGTGATTCAGACACTCAAGAGACACCAGAAGAAAGATGTCGAGGGATTATTGGCTTTCTATGGTCTACCTCTTCCTCATACTCTTGTCGAACTTTCTGCTCCGGCCCCAGTGCCTACATCGCTACCAGATGGAGTCAAGTTTGAGCTGCAGACATTGCCAGTTGATGCAAAAGCTGTAGCAGATGGTGATACAGTGACAGTGTATGTGAGTACAGCACACCCTCGAGAGTCCTCTTGTATTCCAATGGAGGTTAGGAACGCAGCCATTCAAAGATCACAAGCTAGAAACGCCAAGAACTACGCAAAGGCTGATGCACTTCACAAGACAATAACTGATGCTGGTTACAGGGTGCTTACTGTTCAGAATGAGGAGATTCTTGCAAGAAAATACAGGATTCGTCTCAGAGGAATAGATGCACCAGAAAGCGCAATGCCTTATGGGAAAGAGGCAAAAGAAGAGCTGAGAAAGCTTGTTGAAGGCAAGTGTTTGAGAGTACTTGTGTACGGAGAAGATCGGTATAACCGATGTGTGGGTGACTTGTATTGTAATGGAAAGTTTATTCAGGAAGCAATGCTGAAAAAGGGCTTTGCGTGGCATTACACTGCCTATGACAAACGAGCAGAGCTGGCAAAGTGGGAAAATGAAGCTCGAGCTAAAAGGATAGGGTTGTGGGCTTCTAGAAACCCAGAGCAGCCATGGGAATGGAGAAAGGGAAAGCGTGAAGGGAAATAGTATACACAATTTA

Coding sequence (CDS)

ATGGGGAACGTTCTCAGGTTCCTCTGCGGTCACTTCTGCAATCCTAACGCTGCCGCCGACGCCGATTCCCTCGGTCCTCATGGCGTCTCGCCGGCCACCGTCGGCGTCTCGGCTCTGGCTCACGATCTCTTTGATTTCGAGATCACCTCGCAGGTTCCTCAAGGACTTAGTAAGCATGTGGTTTCGTCGAAGAAGGCACAGGCGAATTGGTATAGAAAACTGGCTGAAGCTTGGAGGTCGGAGAAACCACCTCCTAAAACGCCTGAAGAAGCATCCAGGCTTGTGATTCAGACACTCAAGAGACACCAGAAGAAAGATGTCGAGGGATTATTGGCTTTCTATGGTCTACCTCTTCCTCATACTCTTGTCGAACTTTCTGCTCCGGCCCCAGTGCCTACATCGCTACCAGATGGAGTCAAGTTTGAGCTGCAGACATTGCCAGTTGATGCAAAAGCTGTAGCAGATGGTGATACAGTGACAGTGTATGTGAGTACAGCACACCCTCGAGAGTCCTCTTGTATTCCAATGGAGGTTAGGAACGCAGCCATTCAAAGATCACAAGCTAGAAACGCCAAGAACTACGCAAAGGCTGATGCACTTCACAAGACAATAACTGATGCTGGTTACAGGGTGCTTACTGTTCAGAATGAGGAGATTCTTGCAAGAAAATACAGGATTCGTCTCAGAGGAATAGATGCACCAGAAAGCGCAATGCCTTATGGGAAAGAGGCAAAAGAAGAGCTGAGAAAGCTTGTTGAAGGCAAGTGTTTGAGAGTACTTGTGTACGGAGAAGATCGGTATAACCGATGTGTGGGTGACTTGTATTGTAATGGAAAGTTTATTCAGGAAGCAATGCTGAAAAAGGGCTTTGCGTGGCATTACACTGCCTATGACAAACGAGCAGAGCTGGCAAAGTGGGAAAATGAAGCTCGAGCTAAAAGGATAGGGTTGTGGGCTTCTAGAAACCCAGAGCAGCCATGGGAATGGAGAAAGGGAAAGCGTGAAGGGAAATAG

Protein sequence

MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHVVSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPHTLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRNAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPYGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRAELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK
BLAST of Carg24489 vs. NCBI nr
Match: XP_022941859.1 (staphylococcal-like nuclease CAN2 [Cucurbita moschata] >XP_023552645.1 staphylococcal-like nuclease CAN2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 682.6 bits (1760), Expect = 7.0e-193
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0

Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV
Sbjct: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH
Sbjct: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN
Sbjct: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY
Sbjct: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR
Sbjct: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300

Query: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
           AELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK
Sbjct: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 337

BLAST of Carg24489 vs. NCBI nr
Match: XP_022996106.1 (staphylococcal-like nuclease CAN2 [Cucurbita maxima] >XP_022996112.1 staphylococcal-like nuclease CAN2 [Cucurbita maxima])

HSP 1 Score: 674.9 bits (1740), Expect = 1.5e-190
Identity = 332/337 (98.52%), Postives = 334/337 (99.11%), Query Frame = 0

Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGN LRFLC HFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV
Sbjct: 1   MGNALRFLCSHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSSKKAQ NWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH
Sbjct: 61  VSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVELSAPAP+PTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN
Sbjct: 121 TLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY
Sbjct: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR
Sbjct: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300

Query: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
           AELAKWENEARA+RIGLWASRNPEQPWEWRKGKREGK
Sbjct: 301 AELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK 337

BLAST of Carg24489 vs. NCBI nr
Match: XP_022146824.1 (staphylococcal-like nuclease CAN2 [Momordica charantia])

HSP 1 Score: 601.7 bits (1550), Expect = 1.6e-168
Identity = 302/343 (88.05%), Postives = 312/343 (90.96%), Query Frame = 0

Query: 1   MGNVLRFLCGHFCNPNA-----AADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQG 60
           MGN LRFLCGHFC+P+A          SLGPHGVSPATVGVSALAHDLFDFEITSQVPQG
Sbjct: 1   MGNALRFLCGHFCSPSAXXXXXXXXXXSLGPHGVSPATVGVSALAHDLFDFEITSQVPQG 60

Query: 61  LSKHVVSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYG 120
           LSKHVVSSKKAQANWY KLAEAWRSEKPPP+TPEEASRLVIQTLKRHQKKDVEGLLAFYG
Sbjct: 61  LSKHVVSSKKAQANWYGKLAEAWRSEKPPPRTPEEASRLVIQTLKRHQKKDVEGLLAFYG 120

Query: 121 LPLPHTLVELSAPAPVP-TSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCI 180
           LPLPHTLVELSA AP     LPDG+KFELQTLPVDAKAVADGDTVTVYVST +PRES+CI
Sbjct: 121 LPLPHTLVELSASAPTTGAPLPDGIKFELQTLPVDAKAVADGDTVTVYVSTENPRESACI 180

Query: 181 PMEVRNAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAP 240
           P EV+ AA+QRS AR AKNYAKADALHK ITDAGYRVLT QNEEILA+KYRIRLRGIDAP
Sbjct: 181 PKEVQAAAVQRSHARAAKNYAKADALHKKITDAGYRVLTFQNEEILAKKYRIRLRGIDAP 240

Query: 241 ESAMPYGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHY 300
           ES+MPYGKEAKEEL KLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHY
Sbjct: 241 ESSMPYGKEAKEELTKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHY 300

Query: 301 TAYDKRAELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
            AYDKRAELAKWE EARAKR GLWAS NPEQPWEWRK KREGK
Sbjct: 301 AAYDKRAELAKWEKEARAKRAGLWASSNPEQPWEWRKAKREGK 343

BLAST of Carg24489 vs. NCBI nr
Match: XP_008456902.1 (PREDICTED: staphylococcal-like nuclease CAN1 [Cucumis melo])

HSP 1 Score: 597.0 bits (1538), Expect = 3.9e-167
Identity = 291/338 (86.09%), Postives = 314/338 (92.90%), Query Frame = 0

Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPH-GVSPATVGVSALAHDLFDFEITSQVPQGLSKH 60
           MGN LRFLCGHFC+P     +DSL PH  +S +T GVSALAHDLF+FEITSQVP GL KH
Sbjct: 1   MGNALRFLCGHFCHPT----SDSLPPHAALSSSTAGVSALAHDLFEFEITSQVPPGLGKH 60

Query: 61  VVSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLP 120
           VVSSKKAQANWY KLAEAW+SEKP P+TPEEASRLVIQTLKRHQKKDVEGLL FYGLPLP
Sbjct: 61  VVSSKKAQANWYGKLAEAWKSEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLP 120

Query: 121 HTLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVR 180
           HTLV+ SAPAP   S+PDGVKFELQTLPVDAKAVADGDTVTV+VST+HPRESSC+P EVR
Sbjct: 121 HTLVKSSAPAPTAASIPDGVKFELQTLPVDAKAVADGDTVTVHVSTSHPRESSCVPKEVR 180

Query: 181 NAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMP 240
           NAA+QRS+ARNAKNYAKADALHKTITDAGYRV+T QNEE+LA+KYRIRLRGIDAPESAMP
Sbjct: 181 NAALQRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMP 240

Query: 241 YGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300
           YGKEAKEEL++LVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK
Sbjct: 241 YGKEAKEELKRLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300

Query: 301 RAELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
           R EL+KWENEARAKR+GLWAS NP++PWEWRKGKREGK
Sbjct: 301 RPELSKWENEARAKRVGLWASSNPDKPWEWRKGKREGK 334

BLAST of Carg24489 vs. NCBI nr
Match: NP_001275526.1 (uncharacterized protein LOC101213685 [Cucumis sativus] >ABX56707.1 calcium-dependent nuclease [Cucumis sativus] >ACS71330.1 calcium-dependent nuclease [Cucumis sativus] >KGN50709.1 hypothetical protein Csa_5G218750 [Cucumis sativus])

HSP 1 Score: 588.2 bits (1515), Expect = 1.8e-164
Identity = 289/338 (85.50%), Postives = 308/338 (91.12%), Query Frame = 0

Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPH-GVSPATVGVSALAHDLFDFEITSQVPQGLSKH 60
           MGN LRFLCGHF  P     +DS  PH  VS +T GVSAL HDLF+FEITSQVP GLSKH
Sbjct: 1   MGNALRFLCGHFSRPT----SDSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKH 60

Query: 61  VVSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLP 120
           VVSSKKAQANWY KLAEAW++EKP P+TPEEASRLVIQTLKRHQKKDVEGLL FYGLPLP
Sbjct: 61  VVSSKKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLP 120

Query: 121 HTLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVR 180
           HTLV+ SAP P   S+PDGVKFELQTLPVDAKAVADGDTVTVYVST+HPRESSC+P EVR
Sbjct: 121 HTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVR 180

Query: 181 NAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMP 240
           NAA+QRS+ARNAKN+AKADALHKTITDAGYRV+T QNEE+LA+KYRIRLRGIDAPESAMP
Sbjct: 181 NAALQRSRARNAKNFAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMP 240

Query: 241 YGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300
           YGKEAKEEL++LVEGKCLRV VYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK
Sbjct: 241 YGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300

Query: 301 RAELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
           R EL+KWENEARAKR GLWAS NPEQPWEWRKGKREGK
Sbjct: 301 RPELSKWENEARAKRAGLWASSNPEQPWEWRKGKREGK 334

BLAST of Carg24489 vs. TAIR10
Match: AT2G40410.2 (Staphylococcal nuclease homologue)

HSP 1 Score: 477.2 bits (1227), Expect = 8.1e-135
Identity = 237/334 (70.96%), Postives = 272/334 (81.44%), Query Frame = 0

Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGN L FL G  C P      DSLGPHGVS ATVGVSALAHDLF+FEITSQVP+GL ++V
Sbjct: 1   MGNALTFLYGKCCKPTTT--DDSLGPHGVSAATVGVSALAHDLFNFEITSQVPEGLGRYV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
            SS+KAQANWYRK+ EAW+  KPPP+T EEASRLV   LKR+QK DVEGLL+FYGLPL H
Sbjct: 61  QSSRKAQANWYRKILEAWKQAKPPPQTAEEASRLVTDILKRNQKADVEGLLSFYGLPLSH 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVE++  APV  SLP+G+ FE QTLPVD KAVADGDT+TVYVST+ P  SS +P EV  
Sbjct: 121 TLVEVTVEAPV--SLPEGILFEFQTLPVDPKAVADGDTITVYVSTSEPVVSSSVPREVNL 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AA+QR++AR  +NY KAD LH+ I D+GYRVL ++NEE+LARK+RIRLRGIDAPES MP+
Sbjct: 181 AAVQRAKAREKRNYPKADELHQKIIDSGYRVLNIENEEVLARKFRIRLRGIDAPESQMPF 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEA+E L K+V  K L+VLVYGED+Y RCVGDLYCNG F+QEAMLKKG AWHY AYDKR
Sbjct: 241 GKEAQEGLLKIVGRKSLKVLVYGEDQYGRCVGDLYCNGIFVQEAMLKKGLAWHYLAYDKR 300

Query: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKR 335
             LAKWE EAR KRIGLWAS NPE+PW+WRK  R
Sbjct: 301 PVLAKWEKEARQKRIGLWASSNPEKPWDWRKNNR 330

BLAST of Carg24489 vs. TAIR10
Match: AT3G56170.1 (Ca-2+ dependent nuclease)

HSP 1 Score: 455.3 bits (1170), Expect = 3.3e-128
Identity = 226/336 (67.26%), Postives = 263/336 (78.27%), Query Frame = 0

Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGN +R L               L  HGVS ++ GVSAL+ DL +FE TSQVP+ L  +V
Sbjct: 1   MGNAIRLL------------RKCLNSHGVSASSGGVSALSRDLLNFETTSQVPEKLGSYV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSS+KAQANWYRK+ EAW+  KP PKTPEEASRLVI  LK HQK DVEGLL+FYGLP PH
Sbjct: 61  VSSQKAQANWYRKILEAWKQAKPRPKTPEEASRLVIAALKNHQKADVEGLLSFYGLPSPH 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
            LVE+  P   P SLP GV+FEL TLPVD K+VADGDTVTVYVS+  P  SS +P +V  
Sbjct: 121 NLVEV--PTEAPVSLPKGVRFELNTLPVDTKSVADGDTVTVYVSSKDPLVSSSLPKDVSL 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AA++R++AR  KNY +ADALHKTI  +GYR+++ QNEE+LA+K+RIRL GID+PES MPY
Sbjct: 181 AAVKRAKAREKKNYTEADALHKTIIASGYRMISFQNEEVLAKKFRIRLSGIDSPESKMPY 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEA +EL K+VEGKCL+VLVY EDRY RCVGD+YCNGKF+QE MLKKG AWHY AYDKR
Sbjct: 241 GKEAHDELLKMVEGKCLKVLVYTEDRYGRCVGDIYCNGKFVQEVMLKKGLAWHYVAYDKR 300

Query: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKREG 337
           AELAKWENEAR KR+GLWAS NPE+PWEWRK KR G
Sbjct: 301 AELAKWENEARQKRVGLWASSNPEKPWEWRKNKRGG 322

BLAST of Carg24489 vs. Swiss-Prot
Match: sp|F4IH31|CAN2_ARATH (Staphylococcal-like nuclease CAN2 OS=Arabidopsis thaliana OX=3702 GN=CAN2 PE=1 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 1.5e-133
Identity = 237/334 (70.96%), Postives = 272/334 (81.44%), Query Frame = 0

Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGN L FL G  C P      DSLGPHGVS ATVGVSALAHDLF+FEITSQVP+GL ++V
Sbjct: 1   MGNALTFLYGKCCKPTTT--DDSLGPHGVSAATVGVSALAHDLFNFEITSQVPEGLGRYV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
            SS+KAQANWYRK+ EAW+  KPPP+T EEASRLV   LKR+QK DVEGLL+FYGLPL H
Sbjct: 61  QSSRKAQANWYRKILEAWKQAKPPPQTAEEASRLVTDILKRNQKADVEGLLSFYGLPLSH 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVE++  APV  SLP+G+ FE QTLPVD KAVADGDT+TVYVST+ P  SS +P EV  
Sbjct: 121 TLVEVTVEAPV--SLPEGILFEFQTLPVDPKAVADGDTITVYVSTSEPVVSSSVPREVNL 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AA+QR++AR  +NY KAD LH+ I D+GYRVL ++NEE+LARK+RIRLRGIDAPES MP+
Sbjct: 181 AAVQRAKAREKRNYPKADELHQKIIDSGYRVLNIENEEVLARKFRIRLRGIDAPESQMPF 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEA+E L K+V  K L+VLVYGED+Y RCVGDLYCNG F+QEAMLKKG AWHY AYDKR
Sbjct: 241 GKEAQEGLLKIVGRKSLKVLVYGEDQYGRCVGDLYCNGIFVQEAMLKKGLAWHYLAYDKR 300

Query: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKR 335
             LAKWE EAR KRIGLWAS NPE+PW+WRK  R
Sbjct: 301 PVLAKWEKEARQKRIGLWASSNPEKPWDWRKNNR 330

BLAST of Carg24489 vs. Swiss-Prot
Match: sp|F4IZC5|CAN1_ARATH (Staphylococcal-like nuclease CAN1 OS=Arabidopsis thaliana OX=3702 GN=CAN1 PE=1 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 5.9e-127
Identity = 226/336 (67.26%), Postives = 263/336 (78.27%), Query Frame = 0

Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGN +R L               L  HGVS ++ GVSAL+ DL +FE TSQVP+ L  +V
Sbjct: 1   MGNAIRLL------------RKCLNSHGVSASSGGVSALSRDLLNFETTSQVPEKLGSYV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSS+KAQANWYRK+ EAW+  KP PKTPEEASRLVI  LK HQK DVEGLL+FYGLP PH
Sbjct: 61  VSSQKAQANWYRKILEAWKQAKPRPKTPEEASRLVIAALKNHQKADVEGLLSFYGLPSPH 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
            LVE+  P   P SLP GV+FEL TLPVD K+VADGDTVTVYVS+  P  SS +P +V  
Sbjct: 121 NLVEV--PTEAPVSLPKGVRFELNTLPVDTKSVADGDTVTVYVSSKDPLVSSSLPKDVSL 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AA++R++AR  KNY +ADALHKTI  +GYR+++ QNEE+LA+K+RIRL GID+PES MPY
Sbjct: 181 AAVKRAKAREKKNYTEADALHKTIIASGYRMISFQNEEVLAKKFRIRLSGIDSPESKMPY 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEA +EL K+VEGKCL+VLVY EDRY RCVGD+YCNGKF+QE MLKKG AWHY AYDKR
Sbjct: 241 GKEAHDELLKMVEGKCLKVLVYTEDRYGRCVGDIYCNGKFVQEVMLKKGLAWHYVAYDKR 300

Query: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKREG 337
           AELAKWENEAR KR+GLWAS NPE+PWEWRK KR G
Sbjct: 301 AELAKWENEARQKRVGLWASSNPEKPWEWRKNKRGG 322

BLAST of Carg24489 vs. Swiss-Prot
Match: sp|Q39635|Y38K_CAPSE (Uncharacterized 38.1 kDa protein OS=Capnoides sempervirens OX=3464 PE=2 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 1.7e-126
Identity = 227/338 (67.16%), Postives = 264/338 (78.11%), Query Frame = 0

Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGN LRFL GH C P           +GVS ATVGVSALAHDLF FE TSQ+P+GL+K+V
Sbjct: 1   MGNALRFLYGHCCKPTVEDHYQPPHGYGVSTATVGVSALAHDLFHFENTSQIPEGLTKYV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSSKKAQ NWY+KL++AWR  KPPP+T E+A+RL+I TLKRHQK DV+GLL FYGLPL +
Sbjct: 61  VSSKKAQTNWYKKLSQAWREAKPPPQTAEQATRLIILTLKRHQKADVKGLLRFYGLPLSN 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
                +     P     GVKFEL TLPVD KAVADGDTVTVYV+T  PRE+S +P  V+ 
Sbjct: 121 NPSTEATTVVAPPQADQGVKFELHTLPVDVKAVADGDTVTVYVNTEDPREASNLPKSVKV 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLT-VQNEEILARKYRIRLRGIDAPESAMP 240
           AA +R++AR  ++Y KADAL K I DAGYRVL+   NE+ILARKYRIRLRGID+PES+MP
Sbjct: 181 AAQERAKARAVRDYVKADALQKNIVDAGYRVLSGPNNEDILARKYRIRLRGIDSPESSMP 240

Query: 241 YGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300
           +GKEAKEEL KLV GKCL V +Y EDRY R VGD+YCNG+FIQE MLKKG AWHYTAYDK
Sbjct: 241 FGKEAKEELIKLVVGKCLTVHIYEEDRYGRSVGDIYCNGQFIQEKMLKKGLAWHYTAYDK 300

Query: 301 RAELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
           R +L+KWE +ARA R+GLWAS NPE+PWEWRK KR GK
Sbjct: 301 RPQLSKWEEKARAARVGLWASSNPEKPWEWRKNKRNGK 338

BLAST of Carg24489 vs. Swiss-Prot
Match: sp|Q942N7|CAN1_ORYSJ (Probable staphylococcal-like nuclease CAN1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0166100 PE=2 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 9.5e-117
Identity = 211/338 (62.43%), Postives = 252/338 (74.56%), Query Frame = 0

Query: 1   MGN-VLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKH 60
           MGN + RFLCG  C+P     +    PHG  PA   V+AL  D+  FE TSQVP GLS+H
Sbjct: 1   MGNSIYRFLCG-LCSP-----SPEYQPHGAHPA---VAALGRDIQQFEATSQVPDGLSRH 60

Query: 61  VVSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLP 120
           VVSSKKAQANWY+KL   W+  +P P+TPEEA+RLV+ TLK HQK DVEG L FYGLP+P
Sbjct: 61  VVSSKKAQANWYKKLIVTWKKARPTPRTPEEAARLVVTTLKNHQKADVEGFLVFYGLPIP 120

Query: 121 HTLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVR 180
           +      AP       P G KFEL TLPVDAKAVADGDT+TVY+ TA PRES  +P E++
Sbjct: 121 NAAASTPAPHTAHVPKPQGCKFELHTLPVDAKAVADGDTITVYIDTADPRESGNVPREIQ 180

Query: 181 NAAIQRSQARNAKNYAKADALHKTITDAGYR-VLTVQNEEILARKYRIRLRGIDAPESAM 240
            AA +R++AR A++Y KAD L K I DAGYR V   + EE+LA+KYRIRLRGIDAPESAM
Sbjct: 181 KAAAERTRARAARDYQKADGLQKMIADAGYRQVPNARGEEVLAKKYRIRLRGIDAPESAM 240

Query: 241 PYGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYD 300
           PYGKEAKE L K+V+GK L+V VY EDRY RCVGD+YC+G F+QE MLKKG AWHYTAYD
Sbjct: 241 PYGKEAKEALLKMVQGKSLKVYVYDEDRYGRCVGDIYCDGVFVQEQMLKKGCAWHYTAYD 300

Query: 301 KRAELAKWENEARAKRIGLWASRNPEQPWEWRKGKREG 337
           +R ELAKWE +A++ R GLWA+  P++PWEWR+ KR G
Sbjct: 301 QRPELAKWEKQAQSGRKGLWAASRPQKPWEWRRDKRNG 329

BLAST of Carg24489 vs. Swiss-Prot
Match: sp|Q2RBJ2|CAN2_ORYSJ (Probable staphylococcal-like nuclease CAN2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g0109500 PE=2 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 2.0e-98
Identity = 173/323 (53.56%), Postives = 231/323 (71.52%), Query Frame = 0

Query: 15  PNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHVVSSKKAQANWYRKL 74
           P A      LGPHGV+P+TVGV+ALAHDL +FE TS VP GLS+HVVSS+KAQ  WY+KL
Sbjct: 41  PPAPLQQQHLGPHGVTPSTVGVAALAHDLLNFESTSMVPDGLSQHVVSSRKAQVKWYQKL 100

Query: 75  AEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPHTLVELSAPAPVPTS 134
            EA+++  PPPKTP  A++L+ + L   Q+ D+EG+L FY LP+P   +  ++    P+ 
Sbjct: 101 LEAYKNTTPPPKTPANAAQLIARALNMIQRADLEGILEFYNLPIPS--LPTASSNYQPSL 160

Query: 135 LPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRNAAIQRSQARNAKNY 194
           LP+GV+F L TLPV  K + DGD  T YV T  PRES+ +P+EV    I R+QAR  ++Y
Sbjct: 161 LPEGVQFVLNTLPVYDKCIGDGDGFTAYVPTTDPRESANVPLEVHELVIARTQARKCRDY 220

Query: 195 AKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPYGKEAKEELRKLVEG 254
             ADAL  ++ +AGY++++  ++E+LARKYRIR+RGIDAPE  MPYGKE++  L KL+ G
Sbjct: 221 QSADALLSSLDEAGYKIISCSDDEVLARKYRIRMRGIDAPELKMPYGKESRTALVKLIGG 280

Query: 255 KCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRAELAKWENEARAKR 314
           K +++ VY  D++ R VGD+YCN  FIQE MLK G AWH+  YDKR E A+WE EARA  
Sbjct: 281 KSVKIYVYDLDQFGRYVGDIYCNNLFIQEQMLKNGHAWHFKTYDKRPEFARWEREARAAN 340

Query: 315 IGLWASRNPEQPWEWRKGKREGK 338
            GLWAS NPE+PW+WR+ +R  +
Sbjct: 341 RGLWASGNPEKPWDWRRDQRNAR 361

BLAST of Carg24489 vs. TrEMBL
Match: tr|A0A1S3C4Y1|A0A1S3C4Y1_CUCME (staphylococcal-like nuclease CAN1 OS=Cucumis melo OX=3656 GN=LOC103496714 PE=4 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 2.6e-167
Identity = 291/338 (86.09%), Postives = 314/338 (92.90%), Query Frame = 0

Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPH-GVSPATVGVSALAHDLFDFEITSQVPQGLSKH 60
           MGN LRFLCGHFC+P     +DSL PH  +S +T GVSALAHDLF+FEITSQVP GL KH
Sbjct: 1   MGNALRFLCGHFCHPT----SDSLPPHAALSSSTAGVSALAHDLFEFEITSQVPPGLGKH 60

Query: 61  VVSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLP 120
           VVSSKKAQANWY KLAEAW+SEKP P+TPEEASRLVIQTLKRHQKKDVEGLL FYGLPLP
Sbjct: 61  VVSSKKAQANWYGKLAEAWKSEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLP 120

Query: 121 HTLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVR 180
           HTLV+ SAPAP   S+PDGVKFELQTLPVDAKAVADGDTVTV+VST+HPRESSC+P EVR
Sbjct: 121 HTLVKSSAPAPTAASIPDGVKFELQTLPVDAKAVADGDTVTVHVSTSHPRESSCVPKEVR 180

Query: 181 NAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMP 240
           NAA+QRS+ARNAKNYAKADALHKTITDAGYRV+T QNEE+LA+KYRIRLRGIDAPESAMP
Sbjct: 181 NAALQRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMP 240

Query: 241 YGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300
           YGKEAKEEL++LVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK
Sbjct: 241 YGKEAKEELKRLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300

Query: 301 RAELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
           R EL+KWENEARAKR+GLWAS NP++PWEWRKGKREGK
Sbjct: 301 RPELSKWENEARAKRVGLWASSNPDKPWEWRKGKREGK 334

BLAST of Carg24489 vs. TrEMBL
Match: tr|B6CAZ6|B6CAZ6_CUCSA (Calcium-dependent nuclease OS=Cucumis sativus OX=3659 GN=Csa_5G218750 PE=2 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 1.2e-164
Identity = 289/338 (85.50%), Postives = 308/338 (91.12%), Query Frame = 0

Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPH-GVSPATVGVSALAHDLFDFEITSQVPQGLSKH 60
           MGN LRFLCGHF  P     +DS  PH  VS +T GVSAL HDLF+FEITSQVP GLSKH
Sbjct: 1   MGNALRFLCGHFSRPT----SDSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKH 60

Query: 61  VVSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLP 120
           VVSSKKAQANWY KLAEAW++EKP P+TPEEASRLVIQTLKRHQKKDVEGLL FYGLPLP
Sbjct: 61  VVSSKKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLP 120

Query: 121 HTLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVR 180
           HTLV+ SAP P   S+PDGVKFELQTLPVDAKAVADGDTVTVYVST+HPRESSC+P EVR
Sbjct: 121 HTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVR 180

Query: 181 NAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMP 240
           NAA+QRS+ARNAKN+AKADALHKTITDAGYRV+T QNEE+LA+KYRIRLRGIDAPESAMP
Sbjct: 181 NAALQRSRARNAKNFAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMP 240

Query: 241 YGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300
           YGKEAKEEL++LVEGKCLRV VYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK
Sbjct: 241 YGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300

Query: 301 RAELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
           R EL+KWENEARAKR GLWAS NPEQPWEWRKGKREGK
Sbjct: 301 RPELSKWENEARAKRAGLWASSNPEQPWEWRKGKREGK 334

BLAST of Carg24489 vs. TrEMBL
Match: tr|A0A2P5ECL1|A0A2P5ECL1_9ROSA (Aminoacyl-tRNA synthetase, class 1a, anticodon-binding OS=Trema orientalis OX=63057 GN=TorRG33x02_209580 PE=4 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 1.4e-152
Identity = 275/338 (81.36%), Postives = 297/338 (87.87%), Query Frame = 0

Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGNVLR L  H C P     A + GPHGVS ATVGVSALAHD+F FE TSQVP+GLS+HV
Sbjct: 1   MGNVLRLLYAHCCKP-----ATTAGPHGVSVATVGVSALAHDIFQFENTSQVPEGLSEHV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSSKKAQANWYRKL EAWR  KPPPKTPEEASRLVIQTLKRHQK DVEGLLAFYGLPLPH
Sbjct: 61  VSSKKAQANWYRKLGEAWREAKPPPKTPEEASRLVIQTLKRHQKADVEGLLAFYGLPLPH 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVE+S  A VPTS+P GVKFELQTLPVDAKA+ADGDTVTVYVSTA PRES+C+P +V+ 
Sbjct: 121 TLVEVS--AGVPTSVPAGVKFELQTLPVDAKAIADGDTVTVYVSTADPRESACVPRDVKT 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQN-EEILARKYRIRLRGIDAPESAMP 240
           AA+QRS+AR AKNYAKADALHK I DAGYRV+ +QN EEILARKYRIRLRGIDAPES MP
Sbjct: 181 AAVQRSKARAAKNYAKADALHKQIIDAGYRVINLQNDEEILARKYRIRLRGIDAPESKMP 240

Query: 241 YGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300
           YGKEAKEEL KLV+GKCLRVLVY EDRY RCVGD+Y NGKF+QE MLKKGFAWHY AYD+
Sbjct: 241 YGKEAKEELSKLVQGKCLRVLVYDEDRYGRCVGDIYSNGKFVQEIMLKKGFAWHYLAYDQ 300

Query: 301 RAELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
           R ELAKWENEARAKR+GLWAS NPE+PWEWRK KREG+
Sbjct: 301 RQELAKWENEARAKRVGLWASSNPEKPWEWRKDKREGR 331

BLAST of Carg24489 vs. TrEMBL
Match: tr|A0A2P4HGS0|A0A2P4HGS0_QUESU (Staphylococcal-like nuclease can2 OS=Quercus suber OX=58331 GN=CFP56_45817 PE=4 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 1.2e-151
Identity = 272/338 (80.47%), Postives = 294/338 (86.98%), Query Frame = 0

Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGN LRFL      P  A D++SLG HGVS ATVGVSALAHDLF FEITSQVP+GLSKHV
Sbjct: 1   MGNALRFLYEQCFKPTTAGDSESLGHHGVSAATVGVSALAHDLFHFEITSQVPEGLSKHV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSSKKAQANWYRK+ EAWR+ KPPPKTPEEASRLVI+TLKRHQK DVEGLLAFYGLPLPH
Sbjct: 61  VSSKKAQANWYRKILEAWRAAKPPPKTPEEASRLVIETLKRHQKADVEGLLAFYGLPLPH 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLV+LSA A  PTSLP GVK+E QTLPVDAKAVADGDTVTVYVST  PRESSC+P EV+ 
Sbjct: 121 TLVKLSAGA--PTSLPAGVKYEFQTLPVDAKAVADGDTVTVYVSTTDPRESSCVPREVQV 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQ-NEEILARKYRIRLRGIDAPESAMP 240
           AA QRSQAR  KNY KADA+HK I D+GYRVL  Q NEEILARKYRIRLRGIDAPE +MP
Sbjct: 181 AAAQRSQARAEKNYTKADAIHKKIIDSGYRVLNFQNNEEILARKYRIRLRGIDAPEGSMP 240

Query: 241 YGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300
           YGKEAKEEL K+V+GKCLRVL YGEDRY RCVGD+YCNGKF+QE MLKKGFAWHYTAYD+
Sbjct: 241 YGKEAKEELTKIVQGKCLRVLFYGEDRYGRCVGDIYCNGKFVQELMLKKGFAWHYTAYDQ 300

Query: 301 RAELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
           R ELA WE EA+AKR+GLWAS NPE+PWEWRK KREG+
Sbjct: 301 RPELATWEKEAQAKRVGLWASSNPEKPWEWRKDKREGR 336

BLAST of Carg24489 vs. TrEMBL
Match: tr|B9S495|B9S495_RICCO (Uncharacterized protein OS=Ricinus communis OX=3988 GN=RCOM_0688330 PE=4 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 2.0e-151
Identity = 266/337 (78.93%), Postives = 294/337 (87.24%), Query Frame = 0

Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGN LRFL GH C P+   D++SLGPHG+S ATVGVSALA DLF FEI SQVP+GLSKHV
Sbjct: 1   MGNALRFLYGHCCKPSTTGDSESLGPHGISAATVGVSALAQDLFHFEINSQVPEGLSKHV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSSKKAQANWYRKL EAWR  KPPPKTPEEASRLVIQTLKRHQK DVEGLLAFYGLPLPH
Sbjct: 61  VSSKKAQANWYRKLLEAWREAKPPPKTPEEASRLVIQTLKRHQKADVEGLLAFYGLPLPH 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TL++LS  + VPTSLPDGVKFEL TLPVD +AV DGDT++VYVSTA PRESSC+P EV+ 
Sbjct: 121 TLIQLS--SGVPTSLPDGVKFELLTLPVDPRAVPDGDTISVYVSTADPRESSCVPREVQM 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AA+QRS+AR  +NY KAD L K ITDAGYRV++VQNEEILARKYRIRLRGIDAPES MPY
Sbjct: 181 AAVQRSKARAERNYEKADNLQKKITDAGYRVISVQNEEILARKYRIRLRGIDAPESKMPY 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEA+EEL KLV+GKCLR+LVY EDRY+R VGD+YCNG F+QE MLKKG AWHY AYD+R
Sbjct: 241 GKEAQEELVKLVQGKCLRILVYNEDRYDRSVGDIYCNGIFVQEVMLKKGLAWHYAAYDQR 300

Query: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
            ELA WE EARAKRIGLWAS NPE+PWEWRK +REG+
Sbjct: 301 PELASWEKEARAKRIGLWASTNPEEPWEWRKARREGR 335

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022941859.17.0e-193100.00staphylococcal-like nuclease CAN2 [Cucurbita moschata] >XP_023552645.1 staphyloc... [more]
XP_022996106.11.5e-19098.52staphylococcal-like nuclease CAN2 [Cucurbita maxima] >XP_022996112.1 staphylococ... [more]
XP_022146824.11.6e-16888.05staphylococcal-like nuclease CAN2 [Momordica charantia][more]
XP_008456902.13.9e-16786.09PREDICTED: staphylococcal-like nuclease CAN1 [Cucumis melo][more]
NP_001275526.11.8e-16485.50uncharacterized protein LOC101213685 [Cucumis sativus] >ABX56707.1 calcium-depen... [more]
Match NameE-valueIdentityDescription
AT2G40410.28.1e-13570.96Staphylococcal nuclease homologue[more]
AT3G56170.13.3e-12867.26Ca-2+ dependent nuclease[more]
Match NameE-valueIdentityDescription
sp|F4IH31|CAN2_ARATH1.5e-13370.96Staphylococcal-like nuclease CAN2 OS=Arabidopsis thaliana OX=3702 GN=CAN2 PE=1 S... [more]
sp|F4IZC5|CAN1_ARATH5.9e-12767.26Staphylococcal-like nuclease CAN1 OS=Arabidopsis thaliana OX=3702 GN=CAN1 PE=1 S... [more]
sp|Q39635|Y38K_CAPSE1.7e-12667.16Uncharacterized 38.1 kDa protein OS=Capnoides sempervirens OX=3464 PE=2 SV=1[more]
sp|Q942N7|CAN1_ORYSJ9.5e-11762.43Probable staphylococcal-like nuclease CAN1 OS=Oryza sativa subsp. japonica OX=39... [more]
sp|Q2RBJ2|CAN2_ORYSJ2.0e-9853.56Probable staphylococcal-like nuclease CAN2 OS=Oryza sativa subsp. japonica OX=39... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3C4Y1|A0A1S3C4Y1_CUCME2.6e-16786.09staphylococcal-like nuclease CAN1 OS=Cucumis melo OX=3656 GN=LOC103496714 PE=4 S... [more]
tr|B6CAZ6|B6CAZ6_CUCSA1.2e-16485.50Calcium-dependent nuclease OS=Cucumis sativus OX=3659 GN=Csa_5G218750 PE=2 SV=1[more]
tr|A0A2P5ECL1|A0A2P5ECL1_9ROSA1.4e-15281.36Aminoacyl-tRNA synthetase, class 1a, anticodon-binding OS=Trema orientalis OX=63... [more]
tr|A0A2P4HGS0|A0A2P4HGS0_QUESU1.2e-15180.47Staphylococcal-like nuclease can2 OS=Quercus suber OX=58331 GN=CFP56_45817 PE=4 ... [more]
tr|B9S495|B9S495_RICCO2.0e-15178.93Uncharacterized protein OS=Ricinus communis OX=3988 GN=RCOM_0688330 PE=4 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR035437SNase_OB-fold_sf
IPR016071Staphylococal_nuclease_OB-fold
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg24489-RACarg24489-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016071Staphylococcal nuclease (SNase-like), OB-foldSMARTSM00318SNASE_2coord: 202..320
e-value: 9.0E-33
score: 124.9
IPR016071Staphylococcal nuclease (SNase-like), OB-foldPFAMPF00565SNasecoord: 225..319
e-value: 7.5E-28
score: 97.1
IPR016071Staphylococcal nuclease (SNase-like), OB-foldPROSITEPS50830TNASE_3coord: 144..320
score: 31.644
NoneNo IPR availableGENE3DG3DSA:1.20.120.1910coord: 145..213
e-value: 1.0E-5
score: 27.5
NoneNo IPR availablePANTHERPTHR12302:SF4SUBFAMILY NOT NAMEDcoord: 27..335
NoneNo IPR availablePANTHERPTHR12302EBNA2 BINDING PROTEIN P100coord: 27..335
NoneNo IPR availableSUPERFAMILYSSF50199Staphylococcal nucleasecoord: 146..162
coord: 219..327
IPR035437SNase-like, OB-fold superfamilyGENE3DG3DSA:2.40.50.90coord: 214..325
e-value: 2.6E-25
score: 91.0