Cucsa.142550 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.142550
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Cysteine proteinase inhibitor
Location	scaffold01079 : 803886 .. 806009 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCAAATCCATCAATCCATCAATCCATCAATCTCTCTCTGATTTTTAACTGAAGAGATCCTAATGGCCACTGTTGGAGGTATTAAGGAAGTCCCTTCAAACGAGAACAGCATCGAGGTTGATGAACTTGCTCGTTTTGCTGTTGACGAACACAACAAGAAAGAGGTTTGGTTTTCTTTTTCCTATTCCATTTTGTCATTTCTTAATTGGGTTCTGTTTTGTTTTTTGTTTTATCCCTAATGATATGTGGTTTCTGCGTGTATGTACCCTTCTATTTTTGTGCTCTTATGTGTATATTCGTGTTGATTGTGGATACTGGATGGTCATTTTCTTCAATTGACAACGAAGTTTTCTGTATTGCTTTTCAAATGAATAGGAAGAATAATGGGTTGGATAGGGATTGGGATTTAAGTCTTGTAATTTTACTTTCCCATGATAGCTCCTTTTTGTATAAATTGAATGGGATTAATAAGAAAAAAAATTCTCGATGTCTTCTAGATCCAATCCTTGGGTTGGGGAAAGATAGGTGCTCTGGATTGGGACACCAATTCGATGGGAGAGCTACATTGTAGTTAAACAATGACCGTGTTTATTGTCTTACTAGTTTTCTAAACAACATAATTTTATAGAATTAGATAAGTTGTGTTATGAGGAGTACTGGCCAGACTTAATACAGTTAGATGTAGGATTGGAAAACTTGCAAAGGAATCTTGAATTTGTAATCTTTTCTAGGAGTTATTAGGAAAAACAAAAAAATTCTTAGAAGTCACATCTAATGATCCTTAAGAGTCATCTTTTTGCCACAAATATCAGGTAATCAAAAAGTTCTGATTGAAATCATTTGTGTGTGCAGAATTCACTTCTGGAATTCAGCAAGGTTGTGAAGGTGAAGGAGCAGGTGGTCGCTGGCACAGTTTATTACATAACTGTTGAGGTAACTGAGGGAGGTCAGAAGAAGGTTTATGAAGCCAAGATCTGGGTCAAGCAGTGGCAGAATTTTAAGCAGCTTCAAGAGTTCAAGCTTGCTGGTGATGGCTCTGTGGGCTCCTCTGCTTAGTTTGCTAGGTAACCATCAAACTGTTAAGCATTATCATGATACTTCAAAATCTACATCTTAGGCATCTAATACTATATTTTAATGTACATAAAATGCCTCCTTTGCCTGCTCAATCAAAAGCCATGATAATTTCGTTTTAGTCTTTGTGTTATTAGAATTATGCTTGGTGTTTCTTCACCGTTTCTTTGATGTAGTTTTCACATTTTTTTAAACACGTTTGAAGATTAGACAAGTTCTTAAAGATAAATACAAGTTTCAAAAAATTAAAAAGAAAAAATAGATACACATAATAGAAACTTGTGAGTGAGTAATGATTATAAACAATAACGAAAACAAAAAAGTTACTTAAGGAGACCAAATTAGAGCTTGGTTGGCCAATAACGAATTTAAGTCCAACGAGAGGAAGGAATTTTGTCTGAAGTTCATCTGATTTATGTTGTTTGGGCGACTTTAAAGCCTGATTCTAATGCTAGAATTTCATAAATCTGTCTACAAATTTCAGTAGGACCATGATATATACGAACAATGATGTTATGTATCAACTGTAATATTAGCATGCAGTCATTTTTTAGTCGGAGGTTCAAATGCTGATACTTCTATTTGTTGTATTAACTCCTTTACAACATTTACCTTTACACATTTGCTTGCTTGATAATAAGTATAGGGGGTTTGTGTCTGAGTTTTTTGTTTTAAATCTTTTGCTCTGCATATAACATGTTTAGTTAGTGTAAATAATTTTTTGGACAAGTTATTGGATTTGTGTGACACCCTCTAAATGTGATGTGTTTTTGAAATTTAGGGTCCTAAAATCCACGATGATATGCGTGGAATACGAATGGAGCAGAATCCTTTGCTTTAATGTGTAGTGTCTTTGATTTAAATAAATTCTGTAAGCTTATGGAGTATGTATGGATCCATTTATTATCATAATATCAATAGTTGTTGTGAATTATGTGGCTATTGCATAATATATGTATTTTCAGGAAGAAATATCATATACTTACCTTTCCTTTTCCTTCTTTAACCCTTGTGAATAAAATGATATCAAATTATACTTTTGACTAGTTGA

mRNA sequence

CCAAATCCATCAATCCATCAATCCATCAATCTCTCTCTGATTTTTAACTGAAGAGATCCTAATGGCCACTGTTGGAGGTATTAAGGAAGTCCCTTCAAACGAGAACAGCATCGAGGTTGATGAACTTGCTCGTTTTGCTGTTGACGAACACAACAAGAAAGAGAATTCACTTCTGGAATTCAGCAAGGTTGTGAAGGTGAAGGAGCAGGTGGTCGCTGGCACAGTTTATTACATAACTGTTGAGGTAACTGAGGGAGGTCAGAAGAAGGTTTATGAAGCCAAGATCTGGGTCAAGCAGTGGCAGAATTTTAAGCAGCTTCAAGAGTTCAAGCTTGCTGGTGATGGCTCTGTGGGCTCCTCTGCTTAGTTTGCTAGGGTCCTAAAATCCACGATGATATGCGTGGAATACGAATGGAGCAGAATCCTTTGCTTTAATGTGTAGTGTCTTTGATTTAAATAAATTCTGTAAGCTTATGGAGTATGTATGGATCCATTTATTATCATAATATCAATAGTTGTTGTGAATTATGTGGCTATTGCATAATATATGTATTTTCAGGAAGAAATATCATATACTTACCTTTCCTTTTCCTTCTTTAACCCTTGTGAATAAAATGATATCAAATTATACTTTTGACTAGTTGA

Coding sequence (CDS)

ATGGCCACTGTTGGAGGTATTAAGGAAGTCCCTTCAAACGAGAACAGCATCGAGGTTGATGAACTTGCTCGTTTTGCTGTTGACGAACACAACAAGAAAGAGAATTCACTTCTGGAATTCAGCAAGGTTGTGAAGGTGAAGGAGCAGGTGGTCGCTGGCACAGTTTATTACATAACTGTTGAGGTAACTGAGGGAGGTCAGAAGAAGGTTTATGAAGCCAAGATCTGGGTCAAGCAGTGGCAGAATTTTAAGCAGCTTCAAGAGTTCAAGCTTGCTGGTGATGGCTCTGTGGGCTCCTCTGCTTAG

Protein sequence

MATVGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITVEVTEGGQKKVYEAKIWVKQWQNFKQLQEFKLAGDGSVGSSA*

Homology

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BLAST of Cucsa.142550 vs. Swiss-Prot
Match: CYT6_ARATH (Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana GN=CYS6 PE=1 SV=2)

HSP 1 Score: 144.1 bits (362), Expect = 8.7e-34
Identity = 67/101 (66.34%), Postives = 86/101 (85.15%), Query Frame = 1


Query: 1   MATVGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITV 60
           MA VGG+ +VP+N+NS EV+ LARFAVDEHNKKEN+LLEF++VVK KEQVVAGT++++T+
Sbjct: 34  MALVGGVGDVPANQNSGEVESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTL 93

Query: 61  EVTEGGQKKVYEAKIWVKQWQNFKQLQEFKLAGDGSVGSSA 102
           E+ E GQKK+YEAK+WVK W NFK+LQEFK A D    +S+
Sbjct: 94  EILEAGQKKLYEAKVWVKPWLNFKELQEFKPASDAPAITSS 134

[Close]

BLAST of Cucsa.142550 vs. Swiss-Prot
Match: CYTI_VIGUN (Cysteine proteinase inhibitor OS=Vigna unguiculata PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 9.0e-31
Identity = 60/94 (63.83%), Postives = 78/94 (82.98%), Query Frame = 1


Query: 1  MATVGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITV 60
          MA +GG ++V  N+NS+E+D LARFAV+EHNKK+N+LLEF +VV  ++QVV+GT+Y IT+
Sbjct: 1  MAALGGNRDVAGNQNSLEIDSLARFAVEEHNKKQNALLEFGRVVSAQQQVVSGTLYTITL 60

Query: 61 EVTEGGQKKVYEAKIWVKQWQNFKQLQEFKLAGD 95
          E  +GGQKKVYEAK+W K W NFK+LQEFK  GD
Sbjct: 61 EAKDGGQKKVYEAKVWEKPWLNFKELQEFKHVGD 94

[Close]

BLAST of Cucsa.142550 vs. Swiss-Prot
Match: CYT1_MAIZE (Cystatin-1 OS=Zea mays GN=RAMDAZC7 PE=2 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 5.8e-30
Identity = 59/92 (64.13%), Postives = 77/92 (83.70%), Query Frame = 1


Query: 5   GGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITVEVTE 64
           GGIK+VP+NEN +++ ELARFAV+EHN+K N+LL F K+VK K QVVAGT+YY+T+EV +
Sbjct: 42  GGIKDVPANENDLQLQELARFAVNEHNQKANALLGFEKLVKAKTQVVAGTMYYLTIEVKD 101

Query: 65  GGQKKVYEAKIWVKQWQNFKQLQEFKLAGDGS 97
           G  KK+YEAK+W K W+NFKQLQEFK   +G+
Sbjct: 102 GEVKKLYEAKVWEKPWENFKQLQEFKPVEEGA 133

[Close]

BLAST of Cucsa.142550 vs. Swiss-Prot
Match: CYT12_ORYSJ (Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica GN=Os01g0270100 PE=2 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 1.7e-29
Identity = 59/98 (60.20%), Postives = 79/98 (80.61%), Query Frame = 1


Query: 4   VGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITVEVT 63
           +GG  + PS  NS+E D LARFAVDEHNK+EN+LLEF +VV+ KEQVVAGT++++T+E  
Sbjct: 48  LGGAHDAPSAANSVETDALARFAVDEHNKRENALLEFVRVVEAKEQVVAGTLHHLTLEAL 107

Query: 64  EGGQKKVYEAKIWVKQWQNFKQLQEFKLAGDGSVGSSA 102
           E G+KKVYEAK+WVK W +FK+LQEF+  GD +  ++A
Sbjct: 108 EAGRKKVYEAKVWVKPWLDFKELQEFRNTGDATTFTNA 145

[Close]

BLAST of Cucsa.142550 vs. Swiss-Prot
Match: CYTA_HELAN (Cysteine proteinase inhibitor A OS=Helianthus annuus PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 2.5e-28
Identity = 57/75 (76.00%), Postives = 66/75 (88.00%), Query Frame = 1


Query: 16 SIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITVEVTEGGQKKVYEAKI 75
          S+E+DELARFAVDEHNKK+N+LLEF KVV  KEQVVAG +YYIT+E T GG KK YEAK+
Sbjct: 1  SLEIDELARFAVDEHNKKQNALLEFGKVVNTKEQVVAGKMYYITLEATNGGVKKTYEAKV 60

Query: 76 WVKQWQNFKQLQEFK 91
          WVK W+NFK+LQEFK
Sbjct: 61 WVKPWENFKELQEFK 75

[Close]

BLAST of Cucsa.142550 vs. TrEMBL
Match: G0ZS05_CUCMA (Cysteine proteinase inhibitor (Fragment) OS=Cucurbita maxima PE=2 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 1.9e-40
Identity = 82/101 (81.19%), Postives = 94/101 (93.07%), Query Frame = 1


Query: 1   MATVGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITV 60
           MA +GGIKEVP+N+NS+E DELARFAVDEHNKK N+LLEFSKVVKVKEQVV+G +Y+IT+
Sbjct: 1   MALLGGIKEVPANQNSVETDELARFAVDEHNKKANALLEFSKVVKVKEQVVSGAMYHITL 60

Query: 61  EVTEGGQKKVYEAKIWVKQWQNFKQLQEFKLAGDGSVGSSA 102
           E ++GGQKKVYEAKIWVK W+NFKQLQEFKL GDGSVGSSA
Sbjct: 61  EASDGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 101

[Close]

BLAST of Cucsa.142550 vs. TrEMBL
Match: M1C2L8_SOLTU (Cysteine proteinase inhibitor OS=Solanum tuberosum GN=PGSC0003DMG400022691 PE=3 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 2.5e-35
Identity = 70/96 (72.92%), Postives = 87/96 (90.62%), Query Frame = 1


Query: 1  MATVGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITV 60
          MAT+GGI+E   +ENS+E+++LARFAVDEHNKK+N+LLEF KVV VKEQVVAGT+YYIT+
Sbjct: 1  MATLGGIREAGGSENSVEINDLARFAVDEHNKKQNALLEFGKVVNVKEQVVAGTMYYITL 60

Query: 61 EVTEGGQKKVYEAKIWVKQWQNFKQLQEFKLAGDGS 97
          E TEGG+KK YEAK+WVKQWQNFKQ+++FKL GD +
Sbjct: 61 EATEGGKKKAYEAKVWVKQWQNFKQVEDFKLIGDAA 96

[Close]

BLAST of Cucsa.142550 vs. TrEMBL
Match: A0A059TCI1_TOBAC (Cysteine proteinase inhibitor OS=Nicotiana tabacum GN=CYS6 PE=2 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 7.2e-35
Identity = 70/96 (72.92%), Postives = 86/96 (89.58%), Query Frame = 1


Query: 1  MATVGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITV 60
          MAT+GGI+E   +ENS+E+++LARFAVDEHNKK+N+LLEF KVV VKEQVVAGT+YYIT+
Sbjct: 1  MATLGGIREAGGSENSLEINDLARFAVDEHNKKQNALLEFGKVVNVKEQVVAGTMYYITL 60

Query: 61 EVTEGGQKKVYEAKIWVKQWQNFKQLQEFKLAGDGS 97
          E TEGG+KK YEAK+WVK WQNFKQL++FKL GD +
Sbjct: 61 EATEGGKKKAYEAKVWVKPWQNFKQLEDFKLIGDAA 96

[Close]

BLAST of Cucsa.142550 vs. TrEMBL
Match: K4AT98_SOLLC (Cysteine proteinase inhibitor OS=Solanum lycopersicum PE=3 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 1.6e-34
Identity = 69/96 (71.88%), Postives = 86/96 (89.58%), Query Frame = 1


Query: 1   MATVGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITV 60
           MAT+GGI+E   +ENS+E+++LARFAVDEHNKK+N+LLEF KVV VKEQVVAGT+YYIT+
Sbjct: 16  MATLGGIREAGGSENSLEINDLARFAVDEHNKKQNALLEFGKVVNVKEQVVAGTMYYITL 75

Query: 61  EVTEGGQKKVYEAKIWVKQWQNFKQLQEFKLAGDGS 97
           E TEGG+KK YEAK+WVK WQNFKQ+++FKL GD +
Sbjct: 76  EATEGGKKKAYEAKVWVKPWQNFKQVEDFKLIGDAA 111

[Close]

BLAST of Cucsa.142550 vs. TrEMBL
Match: Q8VX72_RUMOB (Cysteine proteinase inhibitor OS=Rumex obtusifolius PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 2.3e-33
Identity = 67/94 (71.28%), Postives = 84/94 (89.36%), Query Frame = 1


Query: 1  MATVGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITV 60
          MAT+GGIK+V  + NS+EV+ LA+FAV++HNKK+N++LEFSKVV  KEQVVAGT+YYIT+
Sbjct: 1  MATIGGIKQVEGSANSLEVESLAKFAVEDHNKKQNAMLEFSKVVNTKEQVVAGTMYYITL 60

Query: 61 EVTEGGQKKVYEAKIWVKQWQNFKQLQEFKLAGD 95
          E T+GG+KKVYEAK+WVK W NFKQ+QEFKL GD
Sbjct: 61 EATDGGKKKVYEAKVWVKPWMNFKQVQEFKLLGD 94

[Close]

BLAST of Cucsa.142550 vs. TAIR10
Match: AT3G12490.2 (AT3G12490.2 cystatin B)

HSP 1 Score: 144.1 bits (362), Expect = 4.9e-35
Identity = 67/101 (66.34%), Postives = 86/101 (85.15%), Query Frame = 1


Query: 1   MATVGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITV 60
           MA VGG+ +VP+N+NS EV+ LARFAVDEHNKKEN+LLEF++VVK KEQVVAGT++++T+
Sbjct: 34  MALVGGVGDVPANQNSGEVESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTL 93

Query: 61  EVTEGGQKKVYEAKIWVKQWQNFKQLQEFKLAGDGSVGSSA 102
           E+ E GQKK+YEAK+WVK W NFK+LQEFK A D    +S+
Sbjct: 94  EILEAGQKKLYEAKVWVKPWLNFKELQEFKPASDAPAITSS 134

[Close]

BLAST of Cucsa.142550 vs. TAIR10
Match: AT2G40880.1 (AT2G40880.1 cystatin A)

HSP 1 Score: 121.3 bits (303), Expect = 3.4e-28
Identity = 52/87 (59.77%), Postives = 71/87 (81.61%), Query Frame = 1


Query: 4   VGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITVEVT 63
           +GG+ ++  N+NS E++ LARFA+ EHNK++N +LEF K+VK +EQVVAGT+Y++T+E  
Sbjct: 35  LGGVHDLRGNQNSGEIESLARFAIQEHNKQQNKILEFKKIVKAREQVVAGTMYHLTLEAK 94

Query: 64  EGGQKKVYEAKIWVKQWQNFKQLQEFK 91
           EG Q K +EAK+WVK W NFKQLQEFK
Sbjct: 95  EGDQTKNFEAKVWVKPWMNFKQLQEFK 121

[Close]

BLAST of Cucsa.142550 vs. TAIR10
Match: AT5G05110.1 (AT5G05110.1 Cystatin/monellin family protein)

HSP 1 Score: 108.2 bits (269), Expect = 3.0e-24
Identity = 49/88 (55.68%), Postives = 69/88 (78.41%), Query Frame = 1


Query: 4   VGGIKEVPSNENS-IEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITVEV 63
           +GG  +  ++ N   E+D++A FAV EHN++EN++LE ++V+K  EQVVAG +Y +T+EV
Sbjct: 45  LGGFSDSKNDWNGGKEIDDIALFAVQEHNRRENAVLELARVLKATEQVVAGKLYRLTLEV 104

Query: 64  TEGGQKKVYEAKIWVKQWQNFKQLQEFK 91
            E G+KK+YEAK+WVK W NFKQLQEFK
Sbjct: 105 IEAGEKKIYEAKVWVKPWMNFKQLQEFK 132

[Close]

BLAST of Cucsa.142550 vs. TAIR10
Match: AT5G12140.1 (AT5G12140.1 cystatin-1)

HSP 1 Score: 105.5 bits (262), Expect = 1.9e-23
Identity = 46/86 (53.49%), Postives = 66/86 (76.74%), Query Frame = 1


Query: 4  VGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITVEVT 63
          VGG++++ +N N ++V+ LARFAVDEHNK EN  LE+ +++  K QVVAGT++++TVEV 
Sbjct: 10 VGGVRDIDANANDLQVESLARFAVDEHNKNENLTLEYKRLLGAKTQVVAGTMHHLTVEVA 69

Query: 64 EGGQKKVYEAKIWVKQWQNFKQLQEF 90
          +G   KVYEAK+  K W+N KQL+ F
Sbjct: 70 DGETNKVYEAKVLEKAWENLKQLESF 95

[Close]

BLAST of Cucsa.142550 vs. TAIR10
Match: AT5G47550.1 (AT5G47550.1 Cystatin/monellin superfamily protein)

HSP 1 Score: 66.2 bits (160), Expect = 1.3e-11
Identity = 39/95 (41.05%), Postives = 53/95 (55.79%), Query Frame = 1


Query: 2   ATVGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITVE 61
           A VGG   + SN    +V E+  FAV E+NK+  S L+F  VV  + QVV+GT Y + V 
Sbjct: 26  ARVGGWSPI-SNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSGTNYRLKVA 85

Query: 62  VTEG-GQKKVYEAKIWVKQWQNFKQLQEFKLAGDG 96
             +G G  K Y A +W K W  F+ L  F+ A +G
Sbjct: 86  ANDGDGVSKNYLAIVWDKPWMKFRNLTSFEPANNG 119

[Close]

BLAST of Cucsa.142550 vs. NCBI nr
Match: gi|449452723|ref|XP_004144108.1| (PREDICTED: cysteine proteinase inhibitor A [Cucumis sativus])

HSP 1 Score: 201.1 bits (510), Expect = 9.6e-49
Identity = 101/101 (100.00%), Postives = 101/101 (100.00%), Query Frame = 1


Query: 1   MATVGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITV 60
           MATVGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITV
Sbjct: 1   MATVGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITV 60

Query: 61  EVTEGGQKKVYEAKIWVKQWQNFKQLQEFKLAGDGSVGSSA 102
           EVTEGGQKKVYEAKIWVKQWQNFKQLQEFKLAGDGSVGSSA
Sbjct: 61  EVTEGGQKKVYEAKIWVKQWQNFKQLQEFKLAGDGSVGSSA 101

[Close]

BLAST of Cucsa.142550 vs. NCBI nr
Match: gi|659100343|ref|XP_008451048.1| (PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo])

HSP 1 Score: 180.6 bits (457), Expect = 1.3e-42
Identity = 92/101 (91.09%), Postives = 96/101 (95.05%), Query Frame = 1


Query: 1   MATVGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITV 60
           MATVGGIK+VPSNENSIE+DELARFAV EHNKKENSLLEFSKVVKVK+QVVAGTVYYITV
Sbjct: 1   MATVGGIKDVPSNENSIEMDELARFAVAEHNKKENSLLEFSKVVKVKQQVVAGTVYYITV 60

Query: 61  EVTEGGQKKVYEAKIWVKQWQNFKQLQEFKLAGDGSVGSSA 102
           EVTEGGQKKVYEAKIWVK W+NFKQLQEFKL  D SVGSSA
Sbjct: 61  EVTEGGQKKVYEAKIWVKHWENFKQLQEFKLV-DASVGSSA 100

[Close]

BLAST of Cucsa.142550 vs. NCBI nr
Match: gi|341605681|gb|AEK84226.1| (cysteine protease inhibitor [Cucurbita maxima])

HSP 1 Score: 172.9 bits (437), Expect = 2.8e-40
Identity = 82/101 (81.19%), Postives = 94/101 (93.07%), Query Frame = 1


Query: 1   MATVGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITV 60
           MA +GGIKEVP+N+NS+E DELARFAVDEHNKK N+LLEFSKVVKVKEQVV+G +Y+IT+
Sbjct: 1   MALLGGIKEVPANQNSVETDELARFAVDEHNKKANALLEFSKVVKVKEQVVSGAMYHITL 60

Query: 61  EVTEGGQKKVYEAKIWVKQWQNFKQLQEFKLAGDGSVGSSA 102
           E ++GGQKKVYEAKIWVK W+NFKQLQEFKL GDGSVGSSA
Sbjct: 61  EASDGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 101

[Close]

BLAST of Cucsa.142550 vs. NCBI nr
Match: gi|565394166|ref|XP_006362733.1| (PREDICTED: cysteine proteinase inhibitor A-like [Solanum tuberosum])

HSP 1 Score: 156.0 bits (393), Expect = 3.5e-35
Identity = 70/96 (72.92%), Postives = 87/96 (90.62%), Query Frame = 1


Query: 1  MATVGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITV 60
          MAT+GGI+E   +ENS+E+++LARFAVDEHNKK+N+LLEF KVV VKEQVVAGT+YYIT+
Sbjct: 1  MATLGGIREAGGSENSVEINDLARFAVDEHNKKQNALLEFGKVVNVKEQVVAGTMYYITL 60

Query: 61 EVTEGGQKKVYEAKIWVKQWQNFKQLQEFKLAGDGS 97
          E TEGG+KK YEAK+WVKQWQNFKQ+++FKL GD +
Sbjct: 61 EATEGGKKKAYEAKVWVKQWQNFKQVEDFKLIGDAA 96

[Close]

BLAST of Cucsa.142550 vs. NCBI nr
Match: gi|698525620|ref|XP_009759630.1| (PREDICTED: cysteine proteinase inhibitor A-like [Nicotiana sylvestris])

HSP 1 Score: 154.5 bits (389), Expect = 1.0e-34
Identity = 70/96 (72.92%), Postives = 86/96 (89.58%), Query Frame = 1


Query: 1  MATVGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITV 60
          MAT+GGI+E   +ENS+E+++LARFAVDEHNKK+N+LLEF KVV VKEQVVAGT+YYIT+
Sbjct: 1  MATLGGIREAGGSENSLEINDLARFAVDEHNKKQNALLEFGKVVNVKEQVVAGTMYYITL 60

Query: 61 EVTEGGQKKVYEAKIWVKQWQNFKQLQEFKLAGDGS 97
          E TEGG+KK YEAK+WVK WQNFKQL++FKL GD +
Sbjct: 61 EATEGGKKKAYEAKVWVKPWQNFKQLEDFKLIGDAA 96

The following BLAST results are available for this feature:

BLAST of Cucsa.142550 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CYT6_ARATH	8.7e-34	66.34	Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana GN=CYS6 PE=1 SV=2	[more]
CYTI_VIGUN	9.0e-31	63.83	Cysteine proteinase inhibitor OS=Vigna unguiculata PE=1 SV=1	[more]
CYT1_MAIZE	5.8e-30	64.13	Cystatin-1 OS=Zea mays GN=RAMDAZC7 PE=2 SV=1	[more]
CYT12_ORYSJ	1.7e-29	60.20	Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica GN=Os01g0270100...	[more]
CYTA_HELAN	2.5e-28	76.00	Cysteine proteinase inhibitor A OS=Helianthus annuus PE=1 SV=1	[more]

BLAST of Cucsa.142550 vs. TrEMBL


Match Name	E-value	Identity	Description
G0ZS05_CUCMA	1.9e-40	81.19	Cysteine proteinase inhibitor (Fragment) OS=Cucurbita maxima PE=2 SV=1	[more]
M1C2L8_SOLTU	2.5e-35	72.92	Cysteine proteinase inhibitor OS=Solanum tuberosum GN=PGSC0003DMG400022691 PE=3 ...	[more]
A0A059TCI1_TOBAC	7.2e-35	72.92	Cysteine proteinase inhibitor OS=Nicotiana tabacum GN=CYS6 PE=2 SV=1	[more]
K4AT98_SOLLC	1.6e-34	71.88	Cysteine proteinase inhibitor OS=Solanum lycopersicum PE=3 SV=1	[more]
Q8VX72_RUMOB	2.3e-33	71.28	Cysteine proteinase inhibitor OS=Rumex obtusifolius PE=2 SV=1	[more]

BLAST of Cucsa.142550 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G12490.2	4.9e-35	66.34	cystatin B	[more]
AT2G40880.1	3.4e-28	59.77	cystatin A	[more]
AT5G05110.1	3.0e-24	55.68	Cystatin/monellin family protein	[more]
AT5G12140.1	1.9e-23	53.49	cystatin-1	[more]
AT5G47550.1	1.3e-11	41.05	Cystatin/monellin superfamily protein	[more]

BLAST of Cucsa.142550 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449452723\|ref\|XP_004144108.1\|	9.6e-49	100.00	PREDICTED: cysteine proteinase inhibitor A [Cucumis sativus]	[more]
gi\|659100343\|ref\|XP_008451048.1\|	1.3e-42	91.09	PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo]	[more]
gi\|341605681\|gb\|AEK84226.1\|	2.8e-40	81.19	cysteine protease inhibitor [Cucurbita maxima]	[more]
gi\|565394166\|ref\|XP_006362733.1\|	3.5e-35	72.92	PREDICTED: cysteine proteinase inhibitor A-like [Solanum tuberosum]	[more]
gi\|698525620\|ref\|XP_009759630.1\|	1.0e-34	72.92	PREDICTED: cysteine proteinase inhibitor A-like [Nicotiana sylvestris]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000010	Cystatin_dom
IPR018073	Prot_inh_cystat_CS
IPR020381	Proteinase inhibitor I25, cystatin, conserved region
IPR027214	Cystatin

Vocabulary: **Molecular Function**
Term	Definition
GO:0004869	cysteine-type endopeptidase inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity
molecular_function	GO:0008233	peptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.142550.1	Cucsa.142550.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	SMART	SM00043	CY_4	coord: 2..92 score: 1.5
IPR018073	Proteinase inhibitor I25, cystatin, conserved site	PROSITE	PS00287	CYSTATIN	coord: 48..61 scor
IPR020381	Proteinase inhibitor I25, cystatin, conserved region	PRODOM	PD001231		coord: 16..96 score: 1.0
IPR020381	Proteinase inhibitor I25, cystatin, conserved region	PFAM	PF16845	SQAPI	coord: 15..90 score: 2.0
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 4..96 score: 1.8
None	No IPR available	GENE3D	G3DSA:3.10.450.10		coord: 4..90 score: 1.5
None	No IPR available	PANTHER	PTHR11413:SF56	CYSTEINE PROTEINASE INHIBITOR 3-RELATED	coord: 4..96 score: 1.8
None	No IPR available	unknown	SSF54403	Cystatin/monellin	coord: 3..93 score: 1.15

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.142550	Cla011086	Watermelon (97103) v1	cgywmB251
Cucsa.142550	MELO3C015607	Melon (DHL92) v3.5.1	cgymeB238
Cucsa.142550	ClCG03G014300	Watermelon (Charleston Gray)	cgywcgB242
Cucsa.142550	CSPI01G29500	Wild cucumber (PI 183967)	cgycpiB208
Cucsa.142550	CmaCh11G007990	Cucurbita maxima (Rimu)	cgycmaB0396
Cucsa.142550	CmoCh11G008050	Cucurbita moschata (Rifu)	cgycmoB0396
Cucsa.142550	Lsi06G004220	Bottle gourd (USVL1VR-Ls)	cgylsiB230
Cucsa.142550	Cp4.1LG04g11650	Cucurbita pepo (Zucchini)	cgycpeB0385
Cucsa.142550	MELO3C015607.2	Melon (DHL92) v3.6.1	cgymedB235
Cucsa.142550	CsaV3_1G041920	Cucumber (Chinese Long) v3	cgycucB213
Cucsa.142550	Cla97C03G064490	Watermelon (97103) v2	cgywmbB241
Cucsa.142550	Bhi02G001428	Wax gourd	cgywgoB282
Cucsa.142550	Carg18021	Silver-seed gourd	carcgyB0212

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.142550	Silver-seed gourd	carcgyB0020
Cucsa.142550	Cucurbita maxima (Rimu)	cgycmaB0395
Cucsa.142550	Cucurbita moschata (Rifu)	cgycmoB0395
Cucsa.142550	Cucumber (Chinese Long) v2	cgycuB205
Cucsa.142550	Cucurbita pepo (Zucchini)	cgycpeB0382