Carg18021 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg18021
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Cysteine proteinase inhibitor
Location	Cucurbita_argyrosperma_scaffold_171 : 442899 .. 445305 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GACGAGTCTGCAGACCAATCAATCCTCCTCGTTTTCAACCGGCTATATAAGATTGAAGTGTTTGAGGCAAGAACTGGATCAATCAATTCATCATTCAATTTCTCTATCTCTTGACTGAATCGAAGAGATCGTAATGGCGCTTCTTGGAGGTATCAAGGAAGTTCCTGCAAACCAGAACAGCGTTGAGGCTGATGAACTTGCTCGTTTCGCTGTTAACGAACATAACAAGAAAGAGGTAATGGTTTTCATTTTCCCTTGAGGTTTTGTATGTTGTTTCATGCGTTTATCTCTTCTGTTTTACCATCGTTTTTGTGTTCTTATGTATATTTGTATCGATTGTCTATGATTGTTTGTGAAAAGAGAGAAAAATGGATCGTGATTTACGTTTAGGTTTTCTTTTGTAATTCGTGTCTTCCACGAAATTTAATTTTTATATAAATTGTCTGGTAATAGGAGGTGATGGATTTTCCAGTTGTTCTTCTACTGGTGTGGTGAAAACCACGGGGTGATTCGTTTTCTTTATTTTGAAGTTCTGATTGAAAACATTTCTACATGTAGAATGCGCTTCTGGAATTTAGCAAAGTTGTGAAAGTGAAGGAGCAGGTAGTTTCCGGCACAATGTATCATATAACTCTTGAAGCTAGCAATGGCGGTCAGAAGAAGGTTTATGAAGCCAAGATCTGGGTCAAGGTGTGGGAAAATTTTAAGCAGCTTCAAGAGTTCAAGCTTGTTGGGGATGGGTCTGTGGGCTCCTCTGCTTAGTTTGCTTGGTAATCACTTGAACTCTTGTTTCTCCATTATCATGATACTCCAAAATGTAAATATTAGGCATCTATACTCCATTTTAATGCGTGTGAGAACTGTCTGATTCATGATCGATTACAATTTCGTTTTAAGTTCTGTTTTTAAAAGTTTAACTTGTTTTCTCACCATTTTTTTGGTATGTTTTCATGATTTTTAGAAACACATTTGAATTCTTAGCCAAAGAACTTCTCTAATAATTTTCTTCTTTTGAAAGAGCATAAAAACATGAATGAGTTATGAGAGTAGTATTTGTAAGCTTGATTTTTTAGAAACATGAAATGAGGAACAAAATGGTTATTAAAGAAGGGTTTAGTGTTTCTCATTCAAGATTGCAAAAGACCATATTAGAGCTTGGATGGCCAATACTCTGTCAATGGCAATTACTACTTTGGCAGAAGGTTGTTAGGAACTTGGTGCAAAAACATGGATGAGCAGCTAAACAAGGAATCCTCATTGTAGGTGCAGTTAAGTAGGATTTCATAATTAATGAGGATCTTGTCCTGGTGAAGCAAATAGTTCGTTTGAAATCTTCTAATGCTCTACTTTTAGAATAGGGATGCCACTACAAGGAAGTTAAGGATATCATTAGAGTATCTTGGCAATTAGATAGGGAGTTTGTTGTGGTATTTGGTTATAAATAGAGGGCGTGCGAAAGGAGGAAGTTAGGCAATTGTTTAGTGCCCAAAGGTTCAAGTGATATCTCATTGCAAGTTATATCCTAGTTTTATTGTCACATATAATTCAATGTAATATGTTGAGGATTATTTGGAGTGAGTCCCGCCTTGGTTAATTTAGTGGAAGATCATGGGTTTATAAGTGAGGAATACTATCTTCATTAGTATGAAGCCTTTTGGAGAAGTCCAAAGTAAAGTCATGAGAGCTTATGCTCAAAGTAGATAATATCATACCATTGTGGAGAGTCGTAATTCATAACACCTTAGAGTCGTATCATTTTGATTCTCTTCACCACATTGAACATGTGAGAAAATAATTTTTTGTGAAGTTAATGTACTTTACGTTGTATTGGGTACATCTAAAAGTTGTTTTAATGCTAGAATTTCATAATCTATAATACTGACTGAATAAGTACCGACACTCACATTGAACATCAATATGACATCTACTGACAATAACGTTTAGGTCAAAGCATAGAGTATTGTAGCGTGATGGTATGCCACTTTTCGAACTCTTAAGGCTTCAAAACTGAGTTTTTCATTTGAAATCTTTGCTTTGCATGCACCATACTTAGTAGTTCAAACTTTTGGACAATTACATGGATCCATGTGGTTGAACACTACAAATCTAATGATGTTTTTTCTGAAATTTAGGGTCTTAATGTCGTGATGGAGGAACCTAGATACAGCTGATGCGTGGAATGTTTAATAACATCAAAGACTACTTACATTTGAGACAAATGGAGCATAATCCCTTTGCTTTCTATGTATAGTGTCTTGAATTTAATAAATTCTGTAAGCTATGGAGTGGATATTTATCATCAATAGTTGTTGTTATGAGGCAATTGAAAAAATATATGTATCTTTCATCAAGACATATCCATCTTATAAATCCTTATTACAAATAAGTAAAGCTACTACTTGTACCT

mRNA sequence

GACGAGTCTGCAGACCAATCAATCCTCCTCGTTTTCAACCGGCTATATAAGATTGAAGTGTTTGAGGCAAGAACTGGATCAATCAATTCATCATTCAATTTCTCTATCTCTTGACTGAATCGAAGAGATCGTAATGGCGCTTCTTGGAGGTATCAAGGAAGTTCCTGCAAACCAGAACAGCGTTGAGGCTGATGAACTTGCTCGTTTCGCTGTTAACGAACATAACAAGAAAGAGAATGCGCTTCTGGAATTTAGCAAAGTTGTGAAAGTGAAGGAGCAGGTAGTTTCCGGCACAATGTATCATATAACTCTTGAAGCTAGCAATGGCGGTCAGAAGAAGGTTTATGAAGCCAAGATCTGGGTCAAGGTGTGGGAAAATTTTAAGCAGCTTCAAGAGTTCAAGCTTGTTGGGGATGGGTCTGTGGGCTCCTCTGCTTAGTTTGCTTGGGTCTTAATGTCGTGATGGAGGAACCTAGATACAGCTGATGCGTGGAATGTTTAATAACATCAAAGACTACTTACATTTGAGACAAATGGAGCATAATCCCTTTGCTTTCTATGTATAGTGTCTTGAATTTAATAAATTCTGTAAGCTATGGAGTGGATATTTATCATCAATAGTTGTTGTTATGAGGCAATTGAAAAAATATATGTATCTTTCATCAAGACATATCCATCTTATAAATCCTTATTACAAATAAGTAAAGCTACTACTTGTACCT

Coding sequence (CDS)

ATGGCGCTTCTTGGAGGTATCAAGGAAGTTCCTGCAAACCAGAACAGCGTTGAGGCTGATGAACTTGCTCGTTTCGCTGTTAACGAACATAACAAGAAAGAGAATGCGCTTCTGGAATTTAGCAAAGTTGTGAAAGTGAAGGAGCAGGTAGTTTCCGGCACAATGTATCATATAACTCTTGAAGCTAGCAATGGCGGTCAGAAGAAGGTTTATGAAGCCAAGATCTGGGTCAAGGTGTGGGAAAATTTTAAGCAGCTTCAAGAGTTCAAGCTTGTTGGGGATGGGTCTGTGGGCTCCTCTGCTTAG

Protein sequence

MALLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITLEASNGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA

Homology

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BLAST of Carg18021 vs. NCBI nr
Match: XP_022932964.1 (cysteine proteinase inhibitor A-like [Cucurbita moschata])

HSP 1 Score: 197.2 bits (500), Expect = 2.7e-47
Identity = 100/101 (99.01%), Postives = 101/101 (100.00%), Query Frame = 0


Query: 1   MALLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITL 60
           MALLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITL
Sbjct: 1   MALLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITL 60

Query: 61  EASNGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 102
           EAS+GGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA
Sbjct: 61  EASDGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 101

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BLAST of Carg18021 vs. NCBI nr
Match: XP_022968636.1 (cysteine proteinase inhibitor A-like [Cucurbita maxima] >AEK84226.1 cysteine protease inhibitor, partial [Cucurbita maxima])

HSP 1 Score: 189.5 bits (480), Expect = 5.6e-45
Identity = 96/101 (95.05%), Postives = 98/101 (97.03%), Query Frame = 0


Query: 1   MALLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITL 60
           MALLGGIKEVPANQNSVE DELARFAV+EHNKK NALLEFSKVVKVKEQVVSG MYHITL
Sbjct: 1   MALLGGIKEVPANQNSVETDELARFAVDEHNKKANALLEFSKVVKVKEQVVSGAMYHITL 60

Query: 61  EASNGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 102
           EAS+GGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA
Sbjct: 61  EASDGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 101

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BLAST of Carg18021 vs. NCBI nr
Match: XP_023530395.1 (cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 189.5 bits (480), Expect = 5.6e-45
Identity = 96/101 (95.05%), Postives = 98/101 (97.03%), Query Frame = 0


Query: 1   MALLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITL 60
           MALLGG KEVPANQNSVE DELARFAV+EHNKK NALLEFSKVVKVKEQVVSGTMYHITL
Sbjct: 1   MALLGGAKEVPANQNSVETDELARFAVDEHNKKANALLEFSKVVKVKEQVVSGTMYHITL 60

Query: 61  EASNGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 102
           EAS+GGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA
Sbjct: 61  EASDGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 101

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BLAST of Carg18021 vs. NCBI nr
Match: XP_004144108.1 (PREDICTED: cysteine proteinase inhibitor A [Cucumis sativus])

HSP 1 Score: 172.6 bits (436), Expect = 7.0e-40
Identity = 83/101 (82.18%), Postives = 95/101 (94.06%), Query Frame = 0


Query: 1   MALLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITL 60
           MA +GGIKEVP+N+NS+E DELARFAV+EHNKKEN+LLEFSKVVKVKEQVV+GT+Y+IT+
Sbjct: 1   MATVGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITV 60

Query: 61  EASNGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 102
           E + GGQKKVYEAKIWVK W+NFKQLQEFKL GDGSVGSSA
Sbjct: 61  EVTEGGQKKVYEAKIWVKQWQNFKQLQEFKLAGDGSVGSSA 101

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BLAST of Carg18021 vs. NCBI nr
Match: XP_022147553.1 (cysteine proteinase inhibitor A-like [Momordica charantia])

HSP 1 Score: 165.6 bits (418), Expect = 8.6e-38
Identity = 83/101 (82.18%), Postives = 91/101 (90.10%), Query Frame = 0


Query: 1   MALLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITL 60
           MA +GGIKEV  NQNSVE D+LARFAV EHNKKENALLEF KVV VKEQVV+G +Y ITL
Sbjct: 1   MANVGGIKEVSGNQNSVEIDDLARFAVAEHNKKENALLEFGKVVNVKEQVVAGAIYFITL 60

Query: 61  EASNGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 102
           EA++GG+KKVYEAKIWVK WENFKQLQEFKL+GDGSVGSSA
Sbjct: 61  EATDGGKKKVYEAKIWVKAWENFKQLQEFKLLGDGSVGSSA 101

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BLAST of Carg18021 vs. TAIR10
Match: AT3G12490.2 (cystatin B)

HSP 1 Score: 139.8 bits (351), Expect = 9.2e-34
Identity = 67/101 (66.34%), Postives = 83/101 (82.18%), Query Frame = 0


Query: 1   MALLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITL 60
           MAL+GG+ +VPANQNS E + LARFAV+EHNKKENALLEF++VVK KEQVV+GT++H+TL
Sbjct: 34  MALVGGVGDVPANQNSGEVESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTL 93

Query: 61  EASNGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 102
           E    GQKK+YEAK+WVK W NFK+LQEFK   D    +S+
Sbjct: 94  EILEAGQKKLYEAKVWVKPWLNFKELQEFKPASDAPAITSS 134

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BLAST of Carg18021 vs. TAIR10
Match: AT2G40880.1 (cystatin A)

HSP 1 Score: 123.2 bits (308), Expect = 8.9e-29
Identity = 56/88 (63.64%), Postives = 71/88 (80.68%), Query Frame = 0


Query: 3   LLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITLEA 62
           +LGG+ ++  NQNS E + LARFA+ EHNK++N +LEF K+VK +EQVV+GTMYH+TLEA
Sbjct: 34  MLGGVHDLRGNQNSGEIESLARFAIQEHNKQQNKILEFKKIVKAREQVVAGTMYHLTLEA 93

Query: 63  SNGGQKKVYEAKIWVKVWENFKQLQEFK 91
             G Q K +EAK+WVK W NFKQLQEFK
Sbjct: 94  KEGDQTKNFEAKVWVKPWMNFKQLQEFK 121

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BLAST of Carg18021 vs. TAIR10
Match: AT5G12140.1 (cystatin-1)

HSP 1 Score: 103.2 bits (256), Expect = 9.5e-23
Identity = 45/92 (48.91%), Postives = 68/92 (73.91%), Query Frame = 0


Query: 3   LLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITLEA 62
           ++GG++++ AN N ++ + LARFAV+EHNK EN  LE+ +++  K QVV+GTM+H+T+E 
Sbjct: 9   IVGGVRDIDANANDLQVESLARFAVDEHNKNENLTLEYKRLLGAKTQVVAGTMHHLTVEV 68

Query: 63  SNGGQKKVYEAKIWVKVWENFKQLQEFKLVGD 95
           ++G   KVYEAK+  K WEN KQL+ F  + D
Sbjct: 69  ADGETNKVYEAKVLEKAWENLKQLESFNHLHD 100

[Close]

BLAST of Carg18021 vs. TAIR10
Match: AT5G05110.1 (Cystatin/monellin family protein)

HSP 1 Score: 102.4 bits (254), Expect = 1.6e-22
Identity = 49/88 (55.68%), Postives = 65/88 (73.86%), Query Frame = 0


Query: 4   LGGIKEVPANQN-SVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITLEA 63
           LGG  +   + N   E D++A FAV EHN++ENA+LE ++V+K  EQVV+G +Y +TLE 
Sbjct: 45  LGGFSDSKNDWNGGKEIDDIALFAVQEHNRRENAVLELARVLKATEQVVAGKLYRLTLEV 104

Query: 64  SNGGQKKVYEAKIWVKVWENFKQLQEFK 91
              G+KK+YEAK+WVK W NFKQLQEFK
Sbjct: 105 IEAGEKKIYEAKVWVKPWMNFKQLQEFK 132

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BLAST of Carg18021 vs. TAIR10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein)

HSP 1 Score: 59.3 bits (142), Expect = 1.6e-09
Identity = 34/86 (39.53%), Postives = 51/86 (59.30%), Query Frame = 0


Query: 6   GIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITLEASNG 65
           G ++   N +  +   +A++A+ EHNK+    L F KVV+   QVVSGT Y + + A +G
Sbjct: 30  GSRKPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVSGTKYDLKIAAKDG 89

Query: 66  GQK-KVYEAKIWVKVWENFKQLQEFK 91
           G K K YEA +  K+W + K L+ FK
Sbjct: 90  GGKIKNYEAVVVEKLWLHSKSLESFK 115

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BLAST of Carg18021 vs. Swiss-Prot
Match: sp|Q8H0X6|CYT6_ARATH (Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=2)

HSP 1 Score: 139.8 bits (351), Expect = 1.7e-32
Identity = 67/101 (66.34%), Postives = 83/101 (82.18%), Query Frame = 0


Query: 1   MALLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITL 60
           MAL+GG+ +VPANQNS E + LARFAV+EHNKKENALLEF++VVK KEQVV+GT++H+TL
Sbjct: 34  MALVGGVGDVPANQNSGEVESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTL 93

Query: 61  EASNGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 102
           E    GQKK+YEAK+WVK W NFK+LQEFK   D    +S+
Sbjct: 94  EILEAGQKKLYEAKVWVKPWLNFKELQEFKPASDAPAITSS 134

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BLAST of Carg18021 vs. Swiss-Prot
Match: sp|Q06445|CYTI_VIGUN (Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 8.2e-32
Identity = 67/94 (71.28%), Postives = 78/94 (82.98%), Query Frame = 0


Query: 1  MALLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITL 60
          MA LGG ++V  NQNS+E D LARFAV EHNKK+NALLEF +VV  ++QVVSGT+Y ITL
Sbjct: 1  MAALGGNRDVAGNQNSLEIDSLARFAVEEHNKKQNALLEFGRVVSAQQQVVSGTLYTITL 60

Query: 61 EASNGGQKKVYEAKIWVKVWENFKQLQEFKLVGD 95
          EA +GGQKKVYEAK+W K W NFK+LQEFK VGD
Sbjct: 61 EAKDGGQKKVYEAKVWEKPWLNFKELQEFKHVGD 94

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BLAST of Carg18021 vs. Swiss-Prot
Match: sp|P31726|CYT1_MAIZE (Cystatin-1 OS=Zea mays OX=4577 GN=RAMDAZC7 PE=2 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 5.9e-30
Identity = 62/94 (65.96%), Postives = 77/94 (81.91%), Query Frame = 0


Query: 3   LLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITLEA 62
           L GGIK+VPAN+N ++  ELARFAVNEHN+K NALL F K+VK K QVV+GTMY++T+E 
Sbjct: 40  LAGGIKDVPANENDLQLQELARFAVNEHNQKANALLGFEKLVKAKTQVVAGTMYYLTIEV 99

Query: 63  SNGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGS 97
            +G  KK+YEAK+W K WENFKQLQEFK V +G+
Sbjct: 100 KDGEVKKLYEAKVWEKPWENFKQLQEFKPVEEGA 133

[Close]

BLAST of Carg18021 vs. Swiss-Prot
Match: sp|Q0JNR2|CYT12_ORYSJ (Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0270100 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 2.9e-29
Identity = 61/99 (61.62%), Postives = 80/99 (80.81%), Query Frame = 0


Query: 3   LLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITLEA 62
           +LGG  + P+  NSVE D LARFAV+EHNK+ENALLEF +VV+ KEQVV+GT++H+TLEA
Sbjct: 47  VLGGAHDAPSAANSVETDALARFAVDEHNKRENALLEFVRVVEAKEQVVAGTLHHLTLEA 106

Query: 63  SNGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 102
              G+KKVYEAK+WVK W +FK+LQEF+  GD +  ++A
Sbjct: 107 LEAGRKKVYEAKVWVKPWLDFKELQEFRNTGDATTFTNA 145

[Close]

BLAST of Carg18021 vs. Swiss-Prot
Match: sp|Q10992|CYTA_HELAN (Cysteine proteinase inhibitor A OS=Helianthus annuus OX=4232 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 7.2e-28
Identity = 60/77 (77.92%), Postives = 68/77 (88.31%), Query Frame = 0


Query: 16 SVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITLEASNGGQKKVYEAKI 75
          S+E DELARFAV+EHNKK+NALLEF KVV  KEQVV+G MY+ITLEA+NGG KK YEAK+
Sbjct: 1  SLEIDELARFAVDEHNKKQNALLEFGKVVNTKEQVVAGKMYYITLEATNGGVKKTYEAKV 60

Query: 76 WVKVWENFKQLQEFKLV 93
          WVK WENFK+LQEFK V
Sbjct: 61 WVKPWENFKELQEFKPV 77

[Close]

BLAST of Carg18021 vs. TrEMBL
Match: tr|G0ZS05|G0ZS05_CUCMA (Cysteine proteinase inhibitor (Fragment) OS=Cucurbita maxima OX=3661 PE=2 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 3.7e-45
Identity = 96/101 (95.05%), Postives = 98/101 (97.03%), Query Frame = 0


Query: 1   MALLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITL 60
           MALLGGIKEVPANQNSVE DELARFAV+EHNKK NALLEFSKVVKVKEQVVSG MYHITL
Sbjct: 1   MALLGGIKEVPANQNSVETDELARFAVDEHNKKANALLEFSKVVKVKEQVVSGAMYHITL 60

Query: 61  EASNGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 102
           EAS+GGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA
Sbjct: 61  EASDGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 101

[Close]

BLAST of Carg18021 vs. TrEMBL
Match: tr|A0A1S3BRD0|A0A1S3BRD0_CUCME (Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103492450 PE=3 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 8.2e-37
Identity = 81/101 (80.20%), Postives = 93/101 (92.08%), Query Frame = 0


Query: 1   MALLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITL 60
           MA +GGIK+VP+N+NS+E DELARFAV EHNKKEN+LLEFSKVVKVK+QVV+GT+Y+IT+
Sbjct: 1   MATVGGIKDVPSNENSIEMDELARFAVAEHNKKENSLLEFSKVVKVKQQVVAGTVYYITV 60

Query: 61  EASNGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 102
           E + GGQKKVYEAKIWVK WENFKQLQEFKLV D SVGSSA
Sbjct: 61  EVTEGGQKKVYEAKIWVKHWENFKQLQEFKLV-DASVGSSA 100

[Close]

BLAST of Carg18021 vs. TrEMBL
Match: tr|A0A2I0JQQ5|A0A2I0JQQ5_PUNGR (Cysteine proteinase inhibitor OS=Punica granatum OX=22663 GN=CRG98_021873 PE=3 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 1.6e-32
Identity = 70/101 (69.31%), Postives = 86/101 (85.15%), Query Frame = 0


Query: 1   MALLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITL 60
           MA  GGI+EV  N+NS+E D LARFAV+EHNKKEN+LL+F KVV  K+QVVSGT+Y++T+
Sbjct: 1   MATAGGIREVGGNENSLEIDALARFAVDEHNKKENSLLQFDKVVNAKQQVVSGTVYYLTV 60

Query: 61  EASNGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 102
           EA++GG KKVYEAKIWVK W NFK+LQEFKL+GD   G+SA
Sbjct: 61  EATDGGAKKVYEAKIWVKPWMNFKELQEFKLIGDAPAGASA 101

[Close]

BLAST of Carg18021 vs. TrEMBL
Match: tr|A0A059TCI1|A0A059TCI1_TOBAC (Cysteine proteinase inhibitor OS=Nicotiana tabacum OX=4097 GN=LOC107761445 PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 8.0e-32
Identity = 67/96 (69.79%), Postives = 85/96 (88.54%), Query Frame = 0


Query: 1  MALLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITL 60
          MA LGGI+E   ++NS+E ++LARFAV+EHNKK+NALLEF KVV VKEQVV+GTMY+ITL
Sbjct: 1  MATLGGIREAGGSENSLEINDLARFAVDEHNKKQNALLEFGKVVNVKEQVVAGTMYYITL 60

Query: 61 EASNGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGS 97
          EA+ GG+KK YEAK+WVK W+NFKQL++FKL+GD +
Sbjct: 61 EATEGGKKKAYEAKVWVKPWQNFKQLEDFKLIGDAA 96

[Close]

BLAST of Carg18021 vs. TrEMBL
Match: tr|A0A2N9FL66|A0A2N9FL66_FAGSY (Cysteine proteinase inhibitor OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS19408 PE=3 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 8.0e-32
Identity = 68/101 (67.33%), Postives = 84/101 (83.17%), Query Frame = 0


Query: 1   MALLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITL 60
           MA +GG+ E+  NQNS+E D LAR+AV+EHNKKEN LLEF KVV  K+QVVSGT+Y++TL
Sbjct: 1   MATVGGVSEIKGNQNSLEIDGLARYAVDEHNKKENTLLEFKKVVNAKQQVVSGTLYYLTL 60

Query: 61  EASNGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 102
           E +NGG+ +VYEAKIW K W NFK++QEFKL+GD S GSSA
Sbjct: 61  EVANGGKNEVYEAKIWEKPWLNFKEVQEFKLIGDASSGSSA 101

The following BLAST results are available for this feature:

BLAST of Carg18021 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022932964.1	2.7e-47	99.01	cysteine proteinase inhibitor A-like [Cucurbita moschata]	[more]
XP_022968636.1	5.6e-45	95.05	cysteine proteinase inhibitor A-like [Cucurbita maxima] >AEK84226.1 cysteine pro...	[more]
XP_023530395.1	5.6e-45	95.05	cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo]	[more]
XP_004144108.1	7.0e-40	82.18	PREDICTED: cysteine proteinase inhibitor A [Cucumis sativus]	[more]
XP_022147553.1	8.6e-38	82.18	cysteine proteinase inhibitor A-like [Momordica charantia]	[more]

BLAST of Carg18021 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G12490.2	9.2e-34	66.34	cystatin B	[more]
AT2G40880.1	8.9e-29	63.64	cystatin A	[more]
AT5G12140.1	9.5e-23	48.91	cystatin-1	[more]
AT5G05110.1	1.6e-22	55.68	Cystatin/monellin family protein	[more]
AT4G16500.1	1.6e-09	39.53	Cystatin/monellin superfamily protein	[more]

BLAST of Carg18021 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q8H0X6\|CYT6_ARATH	1.7e-32	66.34	Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=...	[more]
sp\|Q06445\|CYTI_VIGUN	8.2e-32	71.28	Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1	[more]
sp\|P31726\|CYT1_MAIZE	5.9e-30	65.96	Cystatin-1 OS=Zea mays OX=4577 GN=RAMDAZC7 PE=2 SV=1	[more]
sp\|Q0JNR2\|CYT12_ORYSJ	2.9e-29	61.62	Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os0...	[more]
sp\|Q10992\|CYTA_HELAN	7.2e-28	77.92	Cysteine proteinase inhibitor A OS=Helianthus annuus OX=4232 PE=1 SV=1	[more]

BLAST of Carg18021 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|G0ZS05\|G0ZS05_CUCMA	3.7e-45	95.05	Cysteine proteinase inhibitor (Fragment) OS=Cucurbita maxima OX=3661 PE=2 SV=1	[more]
tr\|A0A1S3BRD0\|A0A1S3BRD0_CUCME	8.2e-37	80.20	Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103492450 PE=3 SV=1	[more]
tr\|A0A2I0JQQ5\|A0A2I0JQQ5_PUNGR	1.6e-32	69.31	Cysteine proteinase inhibitor OS=Punica granatum OX=22663 GN=CRG98_021873 PE=3 S...	[more]
tr\|A0A059TCI1\|A0A059TCI1_TOBAC	8.0e-32	69.79	Cysteine proteinase inhibitor OS=Nicotiana tabacum OX=4097 GN=LOC107761445 PE=2 ...	[more]
tr\|A0A2N9FL66\|A0A2N9FL66_FAGSY	8.0e-32	67.33	Cysteine proteinase inhibitor OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS19408 PE=3...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004869	cysteine-type endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR018073	Prot_inh_cystat_CS
IPR027214	Cystatin
IPR000010	Cystatin_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18021-RA	Carg18021-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	SMART	SM00043	CY_4	coord: 2..92 e-value: 1.1E-32 score: 124.6
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 18..91 e-value: 1.6E-22 score: 79.6
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 5..90 e-value: 7.65612E-15 score: 64.0324
None	No IPR available	GENE3D	G3DSA:3.10.450.650		coord: 1..95 e-value: 7.3E-43 score: 148.0
None	No IPR available	PANTHER	PTHR11413:SF56	CYSTEINE PROTEINASE INHIBITOR 3	coord: 3..94
None	No IPR available	PRODOM	PD001231		coord: 16..96 e-value: 1.0E-24 score: 275.0
None	No IPR available	SUPERFAMILY	SSF54403	Cystatin/monellin	coord: 3..94
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 3..94
IPR018073	Proteinase inhibitor I25, cystatin, conserved site	PROSITE	PS00287	CYSTATIN	coord: 48..61

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg18021	Cla011086	Watermelon (97103) v1	carwmB0163
Carg18021	MELO3C015607	Melon (DHL92) v3.5.1	carmeB0178
Carg18021	ClCG03G014300	Watermelon (Charleston Gray)	carwcgB0165
Carg18021	CSPI01G29500	Wild cucumber (PI 183967)	carcpiB0199
Carg18021	Cucsa.142550	Cucumber (Gy14) v1	carcgyB0212
Carg18021	CmaCh11G007990	Cucurbita maxima (Rimu)	carcmaB0231
Carg18021	CmoCh11G008050	Cucurbita moschata (Rifu)	carcmoB0241
Carg18021	Lsi06G004220	Bottle gourd (USVL1VR-Ls)	carlsiB155
Carg18021	Cp4.1LG04g11650	Cucurbita pepo (Zucchini)	carcpeB0248
Carg18021	CsGy1G028800	Cucumber (Gy14) v2	carcgybB0186
Carg18021	MELO3C015607.2	Melon (DHL92) v3.6.1	carmedB0182
Carg18021	CsaV3_1G041920	Cucumber (Chinese Long) v3	carcucB0199
Carg18021	Cla97C03G064490	Watermelon (97103) v2	carwmbB0178
Carg18021	Bhi02G001428	Wax gourd	carwgoB0221

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg18021	Silver-seed gourd	carcarB017
Carg18021	Silver-seed gourd	carcarB209
Carg18021	Cucurbita maxima (Rimu)	carcmaB0230
Carg18021	Cucurbita moschata (Rifu)	carcmoB0240
Carg18021	Cucurbita pepo (Zucchini)	carcpeB0247
Carg18021	Cucumber (Chinese Long) v2	carcuB0192