BLAST of Cucsa.136750 vs. Swiss-Prot
Match:
PUB1_MEDTR (U-box domain-containing protein 1 OS=Medicago truncatula GN=PUB1 PE=1 SV=1)
HSP 1 Score: 582.8 bits (1501), Expect = 4.2e-165
Identity = 298/400 (74.50%), Postives = 343/400 (85.75%), Query Frame = 1
Query: 185 DDFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALRS 244
D+FRCPISLD MRDPVI+SSGHTYDR SIA+WI+SGHH CPKS QRLIH ALIPNYAL+S
Sbjct: 295 DEFRCPISLDLMRDPVIVSSGHTYDRISIAEWINSGHHTCPKSGQRLIHTALIPNYALKS 354
Query: 245 LMQQWCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVDHISASKAASDAIKMTAEFLV 304
L+ QWC ENN+ MNE ++ S+SKR+ +E +DHIS +KA+ DA+KMTAEFLV
Sbjct: 355 LVHQWCYENNVKMNEAITKNNN-----SSSKRHKNENAIDHISENKASKDAVKMTAEFLV 414
Query: 305 GKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLVTLLKSGDPRIEENAVTAL 364
GKLATGS DIQRQ+AYE+RLLAKTGMDNRR+IAE GAIPFLVTLL S D RI+E+ VTAL
Sbjct: 415 GKLATGSTDIQRQSAYEIRLLAKTGMDNRRIIAEVGAIPFLVTLLVSKDSRIQEHVVTAL 474
Query: 365 FNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPK 424
FNL+I++NNKILI+AAGAIDNI +LE GKTMEARENAAA IYSL+M+D+ K+ IGAS +
Sbjct: 475 FNLSIYDNNKILIMAAGAIDNIVEVLEFGKTMEARENAAAAIYSLSMIDDCKVQIGASSR 534
Query: 425 AIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVSGAVPLLIELLTDDKAGITDD 484
AIPALV LLKEG GKRDAATAL NLA+YN NK IV SGAV LL+ELL DDKAGITDD
Sbjct: 535 AIPALVGLLKEGTIIGKRDAATALFNLAVYNPNKLSIVKSGAVTLLVELLMDDKAGITDD 594
Query: 485 ALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEVAR 544
+L L+++LGCSEGL+EI+ S+ LV LLIDLLRFGS KGK++S+TLLLGLCK+ GE VA
Sbjct: 595 SLAVLAVLLGCSEGLEEIKNSKSLVPLLIDLLRFGSVKGKENSITLLLGLCKEEGELVAM 654
Query: 545 RLLINPRSIPSLQSLAADGSLKARRKADALLRLLNRCCSQ 585
RLL NPRSIPSLQSLAADGSL+ARRKADALLRLLNRCCSQ
Sbjct: 655 RLLANPRSIPSLQSLAADGSLRARRKADALLRLLNRCCSQ 689
HSP 2 Score: 198.7 bits (504), Expect = 1.7e-49
Identity = 105/181 (58.01%), Postives = 135/181 (74.59%), Query Frame = 1
Query: 9 SMMAASSSLFPSGSLLESLILLSNEVAFEEKAPFVHAKTISTMRRRIKLLAFLFEEVQES 68
S M S L P+ SLL+SLIL+SNEV+ +K P V K +S+M RRIKLL+ LFEE+QES
Sbjct: 6 SKMMISPGLLPTESLLDSLILISNEVSSMQKFPLVQIKNVSSMIRRIKLLSSLFEEIQES 65
Query: 69 NSPLPPSSILCLTEVFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFVKEIGRVLD 128
+SPLPPSSILC E+FSVI RVK+L Q C +GS LWSL+Q + ISNQF+ VKE+GR LD
Sbjct: 66 DSPLPPSSILCFIEIFSVITRVKVLIQECTDGSSLWSLIQLDFISNQFFVLVKEMGRALD 125
Query: 129 ILPLSLLKLTDDTREQVELLHSQAKRFEFS--VNAREVQRRDELLQLMS-NKERNYKTKD 187
ILPL+LL + D +EQV+LLH Q+KR E ++ REVQRR+ L ++MS N +N KT +
Sbjct: 126 ILPLNLLNVAQDIKEQVDLLHKQSKRVELELFIDPREVQRRENLFEVMSKNCLQNKKTNN 185
BLAST of Cucsa.136750 vs. Swiss-Prot
Match:
PUB13_ARATH (U-box domain-containing protein 13 OS=Arabidopsis thaliana GN=PUB13 PE=1 SV=1)
HSP 1 Score: 312.4 bits (799), Expect = 1.1e-83
Identity = 187/405 (46.17%), Postives = 258/405 (63.70%), Query Frame = 1
Query: 185 DDFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALRS 244
DDFRCPISL+ MRDPVI+SSG TY+R I +WI+ GH CPK+ Q L L PNY LRS
Sbjct: 258 DDFRCPISLEMMRDPVIVSSGQTYERTCIEKWIEGGHSTCPKTQQALTSTTLTPNYVLRS 317
Query: 245 LMQQWCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVDHISASKAASDAIKMTAEFLV 304
L+ QWC+ N+I EP KP SS + +S+ + ++A K+ E L+
Sbjct: 318 LIAQWCEANDI---EPPKPPSSLRPRK--------------VSSFSSPAEANKI--EDLM 377
Query: 305 GKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLVTLLKSGDPRIEENAVTAL 364
+LA G+P+ QR AA E+RLLAK DNR IAEAGAIP LV LL + D RI+E++VTAL
Sbjct: 378 WRLAYGNPEDQRSAAGEIRLLAKRNADNRVAIAEAGAIPLLVGLLSTPDSRIQEHSVTAL 437
Query: 365 FNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPK 424
NL+I NNK IV+AGAI I +L+ G +MEARENAAAT++SL+++DE K+TIGA
Sbjct: 438 LNLSICENNKGAIVSAGAIPGIVQVLKKG-SMEARENAAATLFSLSVIDENKVTIGAL-G 497
Query: 425 AIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVSGAVPLLIELLTDDKAGITDD 484
AIP LV LL EG GK+DAATAL NL +Y NK + +G +P L LLT+ +G+ D+
Sbjct: 498 AIPPLVVLLNEGTQRGKKDAATALFNLCIYQGNKGKAIRAGVIPTLTRLLTEPGSGMVDE 557
Query: 485 ALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEV-- 544
AL L+++ EG I S + S L++ +R GSP+ ++++ +L+ LC + +
Sbjct: 558 ALAILAILSSHPEGKAIIGSSDAVPS-LVEFIRTGSPRNRENAAAVLVHLCSGDPQHLVE 617
Query: 545 ARRL-LINPRSIPSLQSLAADGSLKARRKADALLRLLNRCCSQSQ 587
A++L L+ P L LA +G+ + +RKA LL ++R Q +
Sbjct: 618 AQKLGLMGP-----LIDLAGNGTDRGKRKAAQLLERISRLAEQQK 635
HSP 2 Score: 72.8 bits (177), Expect = 1.4e-11
Identity = 44/156 (28.21%), Postives = 85/156 (54.49%), Query Frame = 1
Query: 22 SLLESLILLSNEVAFEEKAPFVHAKTISTMRRRIKLLAFLFEEVQESNSPLPPSSILCLT 81
S +SLI + NE+A K + RR+KLL +FEE++ESN P+ ++ L
Sbjct: 7 SAAQSLIDVVNEIAAISDYRITVKKLCYNLARRLKLLVPMFEEIRESNEPISEDTLKTLM 66
Query: 82 EVFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFVKEIGRVLDILPLSLLKLTDDT 141
+ + K + C +GS ++ +++ E ++++ + ++ + L +P L ++D+
Sbjct: 67 NLKEAMCSAKDYLKFCSQGSKIYLVMEREQVTSKLMEVSVKLEQSLSQIPYEELDISDEV 126
Query: 142 REQVELLHSQAKRFEFSVNAREVQRRDELLQLMSNK 178
REQVEL+ SQ +R + V+ + + ++ LQ + NK
Sbjct: 127 REQVELVLSQFRRAKGRVDVSDDELYED-LQSLCNK 161
BLAST of Cucsa.136750 vs. Swiss-Prot
Match:
PUB12_ORYSJ (U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica GN=PUB12 PE=2 SV=1)
HSP 1 Score: 298.9 bits (764), Expect = 1.2e-79
Identity = 173/411 (42.09%), Postives = 249/411 (60.58%), Query Frame = 1
Query: 175 SNKERNYKTKDDFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHM 234
S K R+ D+FRCPISL+ M+DPVI+SSG TY+R I +W+DSGH CPK+ Q L H
Sbjct: 220 SIKHRSPIIPDEFRCPISLELMQDPVIVSSGQTYERSCIQKWLDSGHKTCPKTQQPLSHT 279
Query: 235 ALIPNYALRSLMQQWCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVDHISASKAASD 294
+L PN+ L+SL+ QWC+ N I + + + S K+ DH
Sbjct: 280 SLTPNFVLKSLISQWCEANGIELPK--------NKQNSRDKKAAKSSDYDHAGLVS---- 339
Query: 295 AIKMTAEFLVGKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLVTLLKSGDP 354
L+ +L +G+ D QR AA E+RLLAK ++NR IAEAGAIP LV LL S DP
Sbjct: 340 --------LMNRLRSGNQDEQRAAAGEIRLLAKRNVNNRICIAEAGAIPLLVNLLSSSDP 399
Query: 355 RIEENAVTALFNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAATIYSLTMVDE 414
R +E+AVTAL NL+I NNK IV + AI I +L++G +ME RENAAAT++SL++VDE
Sbjct: 400 RTQEHAVTALLNLSIHENNKASIVDSHAIPKIVEVLKTG-SMETRENAAATLFSLSVVDE 459
Query: 415 FKITIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVSGAVPLLIELL 474
K+TIGA+ AIP L+ LL +G+ GK+DAATA+ NL +Y NK V +G V L+ L
Sbjct: 460 NKVTIGAA-GAIPPLINLLCDGSPRGKKDAATAIFNLCIYQGNKVRAVKAGIVIHLMNFL 519
Query: 475 TDDKAGITDDALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGL 534
D G+ D+AL LS++ G EG I +S + L+++++ GSP+ ++++ +L L
Sbjct: 520 VDPTGGMIDEALSLLSILAGNPEGKIVIARSEP-IPPLVEVIKTGSPRNRENAAAILWLL 579
Query: 535 CKDGGEEVARRLLINPRSIPSLQSLAADGSLKARRKADALLRLLNRCCSQS 586
C E+ +L+ L+ G+ +A+RKA ++L L+++ S
Sbjct: 580 CSADTEQTLAAKAAGVED--ALKELSETGTDRAKRKASSILELMHQANEDS 605
HSP 2 Score: 41.6 bits (96), Expect = 3.5e-02
Identity = 29/104 (27.88%), Postives = 53/104 (50.96%), Query Frame = 1
Query: 51 MRRRIKLLAFLFEEVQESNSPLPPSSILCLTEVFSVIRRVKILTQSCEEGSCLWSLLQTE 110
+ RR++LLA L + + S+S SS L + R L + +GS + ++ +
Sbjct: 26 LSRRVRLLAPLLDHLPASSSS---SSSTPLADALGAARD---LLRKTRDGSKIDQAMRGD 85
Query: 111 SISNQFYQFVKEIGRVLDILPLSLLKLTDDTREQVELLHSQAKR 155
+ ++F ++I LD LP + + + +EQV L+HSQ +R
Sbjct: 86 AFLDEFAGVNRQIHLALDALPYNTFHMPQEVQEQVALVHSQFQR 123
BLAST of Cucsa.136750 vs. Swiss-Prot
Match:
PUB14_ARATH (U-box domain-containing protein 14 OS=Arabidopsis thaliana GN=PUB14 PE=1 SV=1)
HSP 1 Score: 298.1 bits (762), Expect = 2.1e-79
Identity = 209/593 (35.24%), Postives = 321/593 (54.13%), Query Frame = 1
Query: 2 AMDVALSSMMAASSS---------LFPSGSLLESLILLSNEV-AFEEKAPFVHAKTISTM 61
AM +AL S + S LF SL+E ++ E+ A + P+ + +
Sbjct: 71 AMRIALDSSLELFRSVNGGSKLFQLFDRDSLVEKFRDMTVEIEAALSQIPYEKIEVSEEV 130
Query: 62 RRRIKLLAFLFEEVQE--SNSPLPPSSILCLTEVFSVIRRVKILTQSCEEGSCLWSLLQT 121
R +++LL F F+ +E S L S L + E +V+ I+ + + L ++ +
Sbjct: 131 REQVQLLHFQFKRAKERWEESDLQLSHDLAMAE--NVMDPDPIILKRLSQELQLTTIDEL 190
Query: 122 ESISNQFYQFVKEIGRVLDILPLSLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDE 181
+ S+ +++ L D + E + S K V
Sbjct: 191 KKESHAIHEY--------------FLSYDGDPDDCFERMSSLLKNLVDFVTMESSDPDPS 250
Query: 182 LLQLMSNKERNYKTKDDFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVCPKSNQ 241
+ ++ R+ + FRCPISL+ M+DPVI+S+G TY+R SI +W+D+GH CPKS +
Sbjct: 251 TGSRIVSRHRSPVIPEYFRCPISLELMKDPVIVSTGQTYERSSIQKWLDAGHKTCPKSQE 310
Query: 242 RLIHMALIPNYALRSLMQQWCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVDHISAS 301
L+H L PNY L+SL+ WC+ N I + + + ++ S+S
Sbjct: 311 TLLHAGLTPNYVLKSLIALWCESNGIELPQNQGSCRTTKIGGSSS--------------- 370
Query: 302 KAASDAIKMTAEFLVGKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLVTLL 361
SD + L+ KLA G+ + QR AA ELRLLAK +DNR IAEAGAIP LV LL
Sbjct: 371 ---SDCDRTFVLSLLEKLANGTTEQQRAAAGELRLLAKRNVDNRVCIAEAGAIPLLVELL 430
Query: 362 KSGDPRIEENAVTALFNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAATIYSL 421
S DPR +E++VTAL NL+I NK IV AGAI +I +L++G +MEARENAAAT++SL
Sbjct: 431 SSPDPRTQEHSVTALLNLSINEGNKGAIVDAGAITDIVEVLKNG-SMEARENAAATLFSL 490
Query: 422 TMVDEFKITIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVSGAVPL 481
+++DE K+ IGA+ AI AL+ LL+EG GK+DAATA+ NL +Y NK+ V G V
Sbjct: 491 SVIDENKVAIGAA-GAIQALISLLEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGGIVDP 550
Query: 482 LIELLTDDKAGITDDALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDSSLT 541
L LL D G+ D+AL L+++ EG I ++ + +L++++R GSP+ ++++
Sbjct: 551 LTRLLKDAGGGMVDEALAILAILSTNQEGKTAIAEAE-SIPVLVEIIRTGSPRNRENAAA 610
Query: 542 LLLGLCKDGGE--EVARRLLINPRSIPSLQSLAADGSLKARRKADALLRLLNR 581
+L LC E VAR + + +L+ L +G+ +A+RKA +LL L+ +
Sbjct: 611 ILWYLCIGNIERLNVAREV----GADVALKELTENGTDRAKRKAASLLELIQQ 622
HSP 2 Score: 51.2 bits (121), Expect = 4.4e-05
Identity = 33/102 (32.35%), Postives = 51/102 (50.00%), Query Frame = 1
Query: 53 RRIKLLAFLFEEVQESNSPLPPSSILCLTEVFSVIRRVKILTQSCEEGSCLWSLLQTESI 112
RRI LL+ FEE+ + N L I + + L +S GS L+ L +S+
Sbjct: 42 RRITLLSPFFEELIDVNVELKKDQITGFEAMRIALDSSLELFRSVNGGSKLFQLFDRDSL 101
Query: 113 SNQFYQFVKEIGRVLDILPLSLLKLTDDTREQVELLHSQAKR 155
+F EI L +P ++++++ REQV+LLH Q KR
Sbjct: 102 VEKFRDMTVEIEAALSQIPYEKIEVSEEVREQVQLLHFQFKR 143
BLAST of Cucsa.136750 vs. Swiss-Prot
Match:
SL11_ORYSJ (E3 ubiquitin-protein ligase SPL11 OS=Oryza sativa subsp. japonica GN=SPL11 PE=1 SV=2)
HSP 1 Score: 293.1 bits (749), Expect = 6.7e-78
Identity = 171/402 (42.54%), Postives = 251/402 (62.44%), Query Frame = 1
Query: 185 DDFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALRS 244
D+FRCPISL+ M+DPVI+S+G TY+R I +WI SGHH CP + Q++ AL PNY LRS
Sbjct: 275 DEFRCPISLELMKDPVIVSTGQTYERACIEKWIASGHHTCPTTQQKMSTSALTPNYVLRS 334
Query: 245 LMQQWCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVDHISASKAASDAIKMTAEFLV 304
L+ QWC+ N + EP K RS +P S+S+ A+ + L+
Sbjct: 335 LISQWCETNGM---EPPK--------RSTQ----PNKPTPACSSSERAN------IDALL 394
Query: 305 GKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLVTLLKSGDPRIEENAVTAL 364
KL + + QR AA ELRLLAK +NR IAEAGAIP L++LL S D R +E+AVTAL
Sbjct: 395 SKLCSPDTEEQRSAAAELRLLAKRNANNRICIAEAGAIPLLLSLLSSSDLRTQEHAVTAL 454
Query: 365 FNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPK 424
NL+I +NK I+++GA+ +I H+L++G +MEARENAAAT++SL+++DE+K+TIG
Sbjct: 455 LNLSIHEDNKASIISSGAVPSIVHVLKNG-SMEARENAAATLFSLSVIDEYKVTIGGM-G 514
Query: 425 AIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVSGAVPLLIELLTDDKAGITDD 484
AIPALV LL EG+ GK+DAA AL NL +Y NK + +G VPL++ L+T+ + D+
Sbjct: 515 AIPALVVLLGEGSQRGKKDAAAALFNLCIYQGNKGRAIRAGLVPLIMGLVTNPTGALMDE 574
Query: 485 ALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEVAR 544
A+ LS++ EG I + V +L++++ G+P+ ++++ ++L LC V
Sbjct: 575 AMAILSILSSHPEGKAAIGAAEP-VPVLVEMIGSGTPRNRENAAAVMLHLCSGEHHLVHL 634
Query: 545 RLLINPRSIPSLQSLAADGSLKARRKADALLRLLNRCCSQSQ 587
+ L+ LA +G+ + +RKA LL ++R Q Q
Sbjct: 635 ARAQECGIMVPLRELALNGTDRGKRKAVQLLERMSRFLVQQQ 652
HSP 2 Score: 49.7 bits (117), Expect = 1.3e-04
Identity = 34/140 (24.29%), Postives = 71/140 (50.71%), Query Frame = 1
Query: 44 HAKTISTMRRRIKLLAFLFEEVQESN--SPLPPSSILCLTEVFSVIRRVKILTQSCEEGS 103
+ + + + RRI+LL EE++E L + + L + EGS
Sbjct: 49 YRRQLLALSRRIRLLGPFVEELRERRRGEGEGEEEERALAPLADALEAALALLRLGREGS 108
Query: 104 CLWSLLQTESISNQFYQFVKEIGRVLDILPLSLLKLTDDTREQVELLHSQAKRFEFSVNA 163
+ +L+ +S+ +F + ++ + L +P + L ++D+ REQVEL+H+Q KR + ++
Sbjct: 109 RISLVLERDSVMKKFQGVILQLEQALCDIPYNELDISDEVREQVELVHAQLKRAKERIDM 168
Query: 164 REVQRRDELLQLMSNKERNY 182
+ + ++LL + ++NY
Sbjct: 169 PDDEFYNDLLSVY---DKNY 185
BLAST of Cucsa.136750 vs. TrEMBL
Match:
A0A0A0K7R8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G012250 PE=4 SV=1)
HSP 1 Score: 788.1 bits (2034), Expect = 7.4e-225
Identity = 404/404 (100.00%), Postives = 404/404 (100.00%), Query Frame = 1
Query: 185 DDFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALRS 244
DDFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALRS
Sbjct: 285 DDFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALRS 344
Query: 245 LMQQWCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVDHISASKAASDAIKMTAEFLV 304
LMQQWCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVDHISASKAASDAIKMTAEFLV
Sbjct: 345 LMQQWCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVDHISASKAASDAIKMTAEFLV 404
Query: 305 GKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLVTLLKSGDPRIEENAVTAL 364
GKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLVTLLKSGDPRIEENAVTAL
Sbjct: 405 GKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLVTLLKSGDPRIEENAVTAL 464
Query: 365 FNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPK 424
FNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPK
Sbjct: 465 FNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPK 524
Query: 425 AIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVSGAVPLLIELLTDDKAGITDD 484
AIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVSGAVPLLIELLTDDKAGITDD
Sbjct: 525 AIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVSGAVPLLIELLTDDKAGITDD 584
Query: 485 ALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEVAR 544
ALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEVAR
Sbjct: 585 ALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEVAR 644
Query: 545 RLLINPRSIPSLQSLAADGSLKARRKADALLRLLNRCCSQSQPC 589
RLLINPRSIPSLQSLAADGSLKARRKADALLRLLNRCCSQSQPC
Sbjct: 645 RLLINPRSIPSLQSLAADGSLKARRKADALLRLLNRCCSQSQPC 688
BLAST of Cucsa.136750 vs. TrEMBL
Match:
A0A0A0K7R8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G012250 PE=4 SV=1)
HSP 1 Score: 351.7 bits (901), Expect = 1.8e-93
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 1
Query: 1 MAMDVALSSMMAASSSLFPSGSLLESLILLSNEVAFEEKAPFVHAKTISTMRRRIKLLAF 60
MAMDVALSSMMAASSSLFPSGSLLESLILLSNEVAFEEKAPFVHAKTISTMRRRIKLLAF
Sbjct: 1 MAMDVALSSMMAASSSLFPSGSLLESLILLSNEVAFEEKAPFVHAKTISTMRRRIKLLAF 60
Query: 61 LFEEVQESNSPLPPSSILCLTEVFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFV 120
LFEEVQESNSPLPPSSILCLTEVFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFV
Sbjct: 61 LFEEVQESNSPLPPSSILCLTEVFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFV 120
Query: 121 KEIGRVLDILPLSLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDELLQLMSNKERN 180
KEIGRVLDILPLSLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDELLQLMSNKERN
Sbjct: 121 KEIGRVLDILPLSLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDELLQLMSNKERN 180
Query: 181 YKTK 185
YKTK
Sbjct: 181 YKTK 184
HSP 2 Score: 656.0 bits (1691), Expect = 4.4e-185
Identity = 339/442 (76.70%), Postives = 385/442 (87.10%), Query Frame = 1
Query: 147 LLHSQAKRFEFSVNAREV--QRRDELLQLMSNKERNYKTKDDFRCPISLDFMRDPVIISS 206
+L+S++ F + + + V Q ++ S+ + D+FRCPISLD M+DPVII+S
Sbjct: 230 VLYSKSMLFSYGDDDKSVKDQSSNKRFDHSSSSKSLVPIPDEFRCPISLDLMKDPVIIAS 289
Query: 207 GHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALRSLMQQWCQENNINMNEPTKPY 266
G TYDR SIAQWIDSGHHVCPKS QRLIHMALIPNYAL+SL+QQWCQENN+ +NEP
Sbjct: 290 GQTYDRTSIAQWIDSGHHVCPKSGQRLIHMALIPNYALKSLVQQWCQENNVQLNEPAT-- 349
Query: 267 SSFELERSNSKRYLSEEPVDHISASKAASDAIKMTAEFLVGKLATGSPDIQRQAAYELRL 326
+S ELERSNS+R LSE VDHIS +KAA DA+KMTAEFLVGKLATGS DIQRQAAYE+RL
Sbjct: 350 ASSELERSNSRRKLSENAVDHISVAKAAIDAVKMTAEFLVGKLATGSSDIQRQAAYEIRL 409
Query: 327 LAKTGMDNRRMIAEAGAIPFLVTLLKSGDPRIEENAVTALFNLAIFNNNKILIVAAGAID 386
LAKTGMDNRRMIAEAGAIPFLVTLL S DPR++ENAVTAL NL+IFNNNK LI++AGAID
Sbjct: 410 LAKTGMDNRRMIAEAGAIPFLVTLLTSTDPRLQENAVTALLNLSIFNNNKTLIISAGAID 469
Query: 387 NITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPKAIPALVRLLKEGNSAGKRDA 446
NI H+LESGKT EARENAAA I+SL+MVDE+K+ IGA P+AIP LVRLL+EG SAGKRDA
Sbjct: 470 NIIHVLESGKTTEARENAAAAIFSLSMVDEYKVVIGAHPRAIPGLVRLLREGTSAGKRDA 529
Query: 447 ATALCNLALYNANKACIVVSGAVPLLIELLTDDKAGITDDALQALSLVLGCSEGLQEIRK 506
A AL NL++Y+ANKA +VV+GAVPLLI+LLTDDKAGITDDALQ LSLVLGCS+GLQEIRK
Sbjct: 530 AIALFNLSIYSANKARVVVAGAVPLLIDLLTDDKAGITDDALQVLSLVLGCSDGLQEIRK 589
Query: 507 SRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEVARRLLINPRSIPSLQSLAADGS 566
SRVLV LLIDLLRFGS KGK++S+TLLLGLCKDGGEEVARRLL+NPRSIPSLQSLAADGS
Sbjct: 590 SRVLVPLLIDLLRFGSLKGKENSITLLLGLCKDGGEEVARRLLMNPRSIPSLQSLAADGS 649
Query: 567 LKARRKADALLRLLNRCCSQSQ 587
LKARRKADALLRLLNRCCS SQ
Sbjct: 650 LKARRKADALLRLLNRCCSHSQ 669
BLAST of Cucsa.136750 vs. TrEMBL
Match:
T1R384_DATGL (E3 ubiquitin ligase OS=Datisca glomerata GN=PUB1 PE=2 SV=1)
HSP 1 Score: 231.1 bits (588), Expect = 3.5e-57
Identity = 119/173 (68.79%), Postives = 143/173 (82.66%), Query Frame = 1
Query: 13 ASSSLFPSGSLLESLILLSNEVAFEEKAPFVHAKTISTMRRRIKLLAFLFEEVQESNSPL 72
ASS P+GSLLESLI +SNEVA E PFV + ISTMRRRIKLL+FLFEE+QE+NSPL
Sbjct: 2 ASSGFLPTGSLLESLIHISNEVASMENMPFVQVRNISTMRRRIKLLSFLFEEIQEANSPL 61
Query: 73 PPSSILCLTEVFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFVKEIGRVLDILPL 132
PPSSILC TE+FSVIRRVK L Q C+EGS LW+LLQTE I+NQFY VKE+ R LDILPL
Sbjct: 62 PPSSILCFTELFSVIRRVKFLIQGCKEGSSLWNLLQTEFITNQFYVLVKEMVRALDILPL 121
Query: 133 SLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDELLQLMS-NKERNYKTK 185
SLLK+T D REQVELLH+QAK+ E SV+ +++QRR+EL+Q+M+ N E+N + K
Sbjct: 122 SLLKITADVREQVELLHNQAKQVELSVDPQDLQRREELVQVMTKNNEKNSRNK 174
HSP 2 Score: 622.9 bits (1605), Expect = 4.1e-175
Identity = 324/453 (71.52%), Postives = 380/453 (83.89%), Query Frame = 1
Query: 133 SLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDELLQLMSNKERNYKTKDDFRCPIS 192
S++ + + R + EL S + S+N ++ R +++N D+FRCPIS
Sbjct: 243 SMIFIDEPERPKEELKRSSSCSSSSSLN-KKFDRSSSSQSVVAN------IPDEFRCPIS 302
Query: 193 LDFMRDPVIISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALRSLMQQWCQE 252
LD MRDPVI++SGHTYDR SIAQWI+SGHH CPKS QRLIHMALIPNYAL+SLM+QWCQ+
Sbjct: 303 LDLMRDPVIVASGHTYDRNSIAQWINSGHHTCPKSGQRLIHMALIPNYALKSLMEQWCQD 362
Query: 253 NNINMNEPTKPYSSFELERSNSKRYLSEEPVDHISASKAASDAIKMTAEFLVGKLATGSP 312
NNI ++E +K S LERS++KR E VDHISA KAA+DA+KM+AEFLVGKLATGSP
Sbjct: 363 NNILLSE-SKSSSLSGLERSDTKRGQCESAVDHISAVKAAADAVKMSAEFLVGKLATGSP 422
Query: 313 DIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLVTLLKSGDPRIEENAVTALFNLAIFNN 372
DIQRQAAYELRLLAKT MDNRR+I+EAGAIPFLVTLL S DPRI+EN VTAL NL+IFNN
Sbjct: 423 DIQRQAAYELRLLAKTSMDNRRIISEAGAIPFLVTLLSSHDPRIQENVVTALLNLSIFNN 482
Query: 373 NKILIVAAGAIDNITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPKAIPALVRL 432
NKILI+AAGAIDNI ++LESG TMEARENAAA I+SL+MVD+ K+ IGA P+AIPALV L
Sbjct: 483 NKILIMAAGAIDNIIYVLESGMTMEARENAAAAIFSLSMVDDCKVAIGARPRAIPALVGL 542
Query: 433 LKEGNSAGKRDAATALCNLALYNANKACIVVSGAVPLLIELLTDDKAGITDDALQALSLV 492
LKEG GK+DAATAL NLA+YNANK +VV+GA+PLL++LL DDKA ITDDAL L+L+
Sbjct: 543 LKEGTPVGKKDAATALFNLAVYNANKVGVVVAGAIPLLVDLLMDDKAAITDDALAVLALL 602
Query: 493 LGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEVARRLLINPRS 552
LGCSEGL+EIRKS+VLV +L+DLLRFGSPKGKD+S+TLLLGLCKDGGEEV RRLLINPRS
Sbjct: 603 LGCSEGLEEIRKSKVLVPILVDLLRFGSPKGKDNSITLLLGLCKDGGEEVVRRLLINPRS 662
Query: 553 IPSLQSLAADGSLKARRKADALLRLLNRCCSQS 586
IPSLQSLAADGSL+ARRKADALLRLL+RCC QS
Sbjct: 663 IPSLQSLAADGSLRARRKADALLRLLSRCCFQS 687
BLAST of Cucsa.136750 vs. TrEMBL
Match:
W9RRI9_9ROSA (U-box domain-containing protein 13 OS=Morus notabilis GN=L484_009898 PE=4 SV=1)
HSP 1 Score: 220.3 bits (560), Expect = 6.1e-54
Identity = 121/202 (59.90%), Postives = 146/202 (72.28%), Query Frame = 1
Query: 3 MDVALSSMMAASSSLFPSGSLLESLILLSNEVAFEEKAPFVHAKTISTMRRRIKLLAFLF 62
MDVALS+++ S + P+GSLLESL+ +S EV EK P V + IS M RRIKLL+ LF
Sbjct: 1 MDVALSAVII-SEAFLPTGSLLESLVHISKEVCSMEKLPIVQVRNISNMTRRIKLLSSLF 60
Query: 63 EEVQESNSPLPPSSILCLTEVFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFVKE 122
EE+QE+N LPPSSILCLTE+FSVIRRVK QSCE+GS LW L+QTE +SNQFY VKE
Sbjct: 61 EEMQETNRSLPPSSILCLTELFSVIRRVKFRIQSCEDGSSLWGLMQTELVSNQFYVLVKE 120
Query: 123 IGRVLDILPLSLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDELLQLMSNKERNYK 182
+G LDILPLSLL ++ D REQVELLH QAKR E ++ RE+QRR+ELLQLM+ N
Sbjct: 121 MGSALDILPLSLLNVSADIREQVELLHKQAKRAELYIDLRELQRREELLQLMATNNENSS 180
Query: 183 TKDDFRCPISLDFMRDPVIISS 205
K F LDF + I+SS
Sbjct: 181 KKKGF-----LDFGKVREILSS 196
HSP 2 Score: 622.5 bits (1604), Expect = 5.4e-175
Identity = 316/401 (78.80%), Postives = 359/401 (89.53%), Query Frame = 1
Query: 185 DDFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALRS 244
D+FRCPISLD MRDPVI++SGHTYDR SIAQWI++GH+ CPKS +LIHMALIPNYAL+S
Sbjct: 284 DEFRCPISLDLMRDPVIVASGHTYDRNSIAQWINTGHNTCPKSGMKLIHMALIPNYALKS 343
Query: 245 LMQQWCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVDHISASKAASDAIKMTAEFLV 304
L+ QWC+ENNI + E T SS +L RSNS R E+ VDHISA+K A DA+KMTAEFLV
Sbjct: 344 LVHQWCRENNIQLIESTSSSSS-DLGRSNSMRKSCEKAVDHISATKTAMDAVKMTAEFLV 403
Query: 305 GKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLVTLLKSGDPRIEENAVTAL 364
GKLATGSP+IQRQAAYELRLLAKTGMDNRR+IAEAGAIPFLVTLL S DPRI+ENAVTAL
Sbjct: 404 GKLATGSPEIQRQAAYELRLLAKTGMDNRRIIAEAGAIPFLVTLLSSHDPRIQENAVTAL 463
Query: 365 FNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPK 424
NL+IF+NNKILI+AAGAIDNI +L+SGKTMEARENAAA I+SL+M+D+ K+TIGA P+
Sbjct: 464 LNLSIFDNNKILIMAAGAIDNIVDVLQSGKTMEARENAAAAIFSLSMIDDCKVTIGAHPR 523
Query: 425 AIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVSGAVPLLIELLTDDKAGITDD 484
A+PALV LL+EG SAGKRDAATAL NL +Y+ANK VV+GAVPLLIELL DDKAGITDD
Sbjct: 524 AMPALVALLREGTSAGKRDAATALFNLVVYSANKGSAVVAGAVPLLIELLMDDKAGITDD 583
Query: 485 ALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEVAR 544
AL L+L+LGC +GL+EIRKSR+LV LLIDLLRFGSPKGK++S+TLLLGLCKDGGEEVAR
Sbjct: 584 ALAVLALLLGCPDGLEEIRKSRILVPLLIDLLRFGSPKGKENSITLLLGLCKDGGEEVAR 643
Query: 545 RLLINPRSIPSLQSLAADGSLKARRKADALLRLLNRCCSQS 586
RLL+NPRSIPSLQSL ADGSLKARRKADALLRLLNRCCSQS
Sbjct: 644 RLLLNPRSIPSLQSLVADGSLKARRKADALLRLLNRCCSQS 683
BLAST of Cucsa.136750 vs. TrEMBL
Match:
F6HCB0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0067g01460 PE=4 SV=1)
HSP 1 Score: 221.5 bits (563), Expect = 2.8e-54
Identity = 121/189 (64.02%), Postives = 146/189 (77.25%), Query Frame = 1
Query: 3 MDVALSSMMAASSSLFPSGSLLESLILLSNEVAFEEKAPFVHAKTISTMRRRIKLLAFLF 62
MDVAL ++M SS P+GSLLESLI +SNEVA E+ P + + ISTM RRIKLL+ LF
Sbjct: 1 MDVALPTLMV-SSGFLPTGSLLESLIHISNEVASMERLPVIQVRNISTMIRRIKLLSSLF 60
Query: 63 EEVQESNSPLPPSSILCLTEVFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFVKE 122
EE+ E N+PLPPSSILCLTE+FSVIRRVK Q C++ S LWSL+QTE IS+QF+ VKE
Sbjct: 61 EEIHEMNTPLPPSSILCLTELFSVIRRVKSFIQGCKDASSLWSLVQTELISSQFHVVVKE 120
Query: 123 IGRVLDILPLSLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDELLQLM-SNKERNY 182
+GR LDILPLSLL +T D REQVELLH QAKR + V+ RE+QRR+ELLQ+M +N E+N
Sbjct: 121 MGRALDILPLSLLNITADIREQVELLHKQAKRVDLFVDPRELQRREELLQVMATNNEKNA 180
Query: 183 KTKD--DFR 189
K K DFR
Sbjct: 181 KNKALVDFR 188
HSP 2 Score: 622.1 bits (1603), Expect = 7.0e-175
Identity = 316/401 (78.80%), Postives = 357/401 (89.03%), Query Frame = 1
Query: 185 DDFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALRS 244
D+FRCPISLD MRDPVI++SGHTYDR SIAQWI+SGHH CPKS QRLIHMALIPNY L+S
Sbjct: 283 DEFRCPISLDLMRDPVIVASGHTYDRNSIAQWINSGHHTCPKSGQRLIHMALIPNYTLKS 342
Query: 245 LMQQWCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVDHISASKAASDAIKMTAEFLV 304
L+ QWCQ+NN+ + E SS +LERSN+K L E +DHISA KAA+DA+KMTAEFLV
Sbjct: 343 LLHQWCQDNNVPLVESASS-SSTDLERSNNKGKLCENAIDHISAMKAAADAVKMTAEFLV 402
Query: 305 GKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLVTLLKSGDPRIEENAVTAL 364
GKLA GSP+IQ QAAYELRLLAKTGMDNRR+IAEAGAIPFLVTLL S DPRI+ENAVTAL
Sbjct: 403 GKLAMGSPEIQSQAAYELRLLAKTGMDNRRIIAEAGAIPFLVTLLSSHDPRIQENAVTAL 462
Query: 365 FNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPK 424
NL+IF+NNKILI+AAGAID+I +L+SGKTMEARENAAATI+SL+M+D+ K+ IG P+
Sbjct: 463 LNLSIFDNNKILIMAAGAIDSIIEVLQSGKTMEARENAAATIFSLSMIDDCKVMIGGRPR 522
Query: 425 AIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVSGAVPLLIELLTDDKAGITDD 484
AIPALV LL+EG +AGK+DAATAL NLA+YNANKA +VV+GAVPLLIELL DDKAGITDD
Sbjct: 523 AIPALVGLLREGTTAGKKDAATALFNLAVYNANKASVVVAGAVPLLIELLMDDKAGITDD 582
Query: 485 ALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEVAR 544
AL L+L+LGC EGL+EIRK RVLV LLIDLLRFGS KGK++S+TLLLGLCKDGGEEVAR
Sbjct: 583 ALAVLALLLGCREGLEEIRKCRVLVPLLIDLLRFGSAKGKENSITLLLGLCKDGGEEVAR 642
Query: 545 RLLINPRSIPSLQSLAADGSLKARRKADALLRLLNRCCSQS 586
RLLINPRSIPSLQSL DGSLKARRKADALLRLLNRCCSQS
Sbjct: 643 RLLINPRSIPSLQSLTTDGSLKARRKADALLRLLNRCCSQS 682
BLAST of Cucsa.136750 vs. TAIR10
Match:
AT3G46510.1 (AT3G46510.1 plant U-box 13)
HSP 1 Score: 312.4 bits (799), Expect = 6.0e-85
Identity = 187/405 (46.17%), Postives = 258/405 (63.70%), Query Frame = 1
Query: 185 DDFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALRS 244
DDFRCPISL+ MRDPVI+SSG TY+R I +WI+ GH CPK+ Q L L PNY LRS
Sbjct: 258 DDFRCPISLEMMRDPVIVSSGQTYERTCIEKWIEGGHSTCPKTQQALTSTTLTPNYVLRS 317
Query: 245 LMQQWCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVDHISASKAASDAIKMTAEFLV 304
L+ QWC+ N+I EP KP SS + +S+ + ++A K+ E L+
Sbjct: 318 LIAQWCEANDI---EPPKPPSSLRPRK--------------VSSFSSPAEANKI--EDLM 377
Query: 305 GKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLVTLLKSGDPRIEENAVTAL 364
+LA G+P+ QR AA E+RLLAK DNR IAEAGAIP LV LL + D RI+E++VTAL
Sbjct: 378 WRLAYGNPEDQRSAAGEIRLLAKRNADNRVAIAEAGAIPLLVGLLSTPDSRIQEHSVTAL 437
Query: 365 FNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPK 424
NL+I NNK IV+AGAI I +L+ G +MEARENAAAT++SL+++DE K+TIGA
Sbjct: 438 LNLSICENNKGAIVSAGAIPGIVQVLKKG-SMEARENAAATLFSLSVIDENKVTIGAL-G 497
Query: 425 AIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVSGAVPLLIELLTDDKAGITDD 484
AIP LV LL EG GK+DAATAL NL +Y NK + +G +P L LLT+ +G+ D+
Sbjct: 498 AIPPLVVLLNEGTQRGKKDAATALFNLCIYQGNKGKAIRAGVIPTLTRLLTEPGSGMVDE 557
Query: 485 ALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEV-- 544
AL L+++ EG I S + S L++ +R GSP+ ++++ +L+ LC + +
Sbjct: 558 ALAILAILSSHPEGKAIIGSSDAVPS-LVEFIRTGSPRNRENAAAVLVHLCSGDPQHLVE 617
Query: 545 ARRL-LINPRSIPSLQSLAADGSLKARRKADALLRLLNRCCSQSQ 587
A++L L+ P L LA +G+ + +RKA LL ++R Q +
Sbjct: 618 AQKLGLMGP-----LIDLAGNGTDRGKRKAAQLLERISRLAEQQK 635
HSP 2 Score: 72.8 bits (177), Expect = 8.0e-13
Identity = 44/156 (28.21%), Postives = 85/156 (54.49%), Query Frame = 1
Query: 22 SLLESLILLSNEVAFEEKAPFVHAKTISTMRRRIKLLAFLFEEVQESNSPLPPSSILCLT 81
S +SLI + NE+A K + RR+KLL +FEE++ESN P+ ++ L
Sbjct: 7 SAAQSLIDVVNEIAAISDYRITVKKLCYNLARRLKLLVPMFEEIRESNEPISEDTLKTLM 66
Query: 82 EVFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFVKEIGRVLDILPLSLLKLTDDT 141
+ + K + C +GS ++ +++ E ++++ + ++ + L +P L ++D+
Sbjct: 67 NLKEAMCSAKDYLKFCSQGSKIYLVMEREQVTSKLMEVSVKLEQSLSQIPYEELDISDEV 126
Query: 142 REQVELLHSQAKRFEFSVNAREVQRRDELLQLMSNK 178
REQVEL+ SQ +R + V+ + + ++ LQ + NK
Sbjct: 127 REQVELVLSQFRRAKGRVDVSDDELYED-LQSLCNK 161
BLAST of Cucsa.136750 vs. TAIR10
Match:
AT3G54850.1 (AT3G54850.1 plant U-box 14)
HSP 1 Score: 298.1 bits (762), Expect = 1.2e-80
Identity = 209/593 (35.24%), Postives = 321/593 (54.13%), Query Frame = 1
Query: 2 AMDVALSSMMAASSS---------LFPSGSLLESLILLSNEV-AFEEKAPFVHAKTISTM 61
AM +AL S + S LF SL+E ++ E+ A + P+ + +
Sbjct: 71 AMRIALDSSLELFRSVNGGSKLFQLFDRDSLVEKFRDMTVEIEAALSQIPYEKIEVSEEV 130
Query: 62 RRRIKLLAFLFEEVQE--SNSPLPPSSILCLTEVFSVIRRVKILTQSCEEGSCLWSLLQT 121
R +++LL F F+ +E S L S L + E +V+ I+ + + L ++ +
Sbjct: 131 REQVQLLHFQFKRAKERWEESDLQLSHDLAMAE--NVMDPDPIILKRLSQELQLTTIDEL 190
Query: 122 ESISNQFYQFVKEIGRVLDILPLSLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDE 181
+ S+ +++ L D + E + S K V
Sbjct: 191 KKESHAIHEY--------------FLSYDGDPDDCFERMSSLLKNLVDFVTMESSDPDPS 250
Query: 182 LLQLMSNKERNYKTKDDFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVCPKSNQ 241
+ ++ R+ + FRCPISL+ M+DPVI+S+G TY+R SI +W+D+GH CPKS +
Sbjct: 251 TGSRIVSRHRSPVIPEYFRCPISLELMKDPVIVSTGQTYERSSIQKWLDAGHKTCPKSQE 310
Query: 242 RLIHMALIPNYALRSLMQQWCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVDHISAS 301
L+H L PNY L+SL+ WC+ N I + + + ++ S+S
Sbjct: 311 TLLHAGLTPNYVLKSLIALWCESNGIELPQNQGSCRTTKIGGSSS--------------- 370
Query: 302 KAASDAIKMTAEFLVGKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLVTLL 361
SD + L+ KLA G+ + QR AA ELRLLAK +DNR IAEAGAIP LV LL
Sbjct: 371 ---SDCDRTFVLSLLEKLANGTTEQQRAAAGELRLLAKRNVDNRVCIAEAGAIPLLVELL 430
Query: 362 KSGDPRIEENAVTALFNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAATIYSL 421
S DPR +E++VTAL NL+I NK IV AGAI +I +L++G +MEARENAAAT++SL
Sbjct: 431 SSPDPRTQEHSVTALLNLSINEGNKGAIVDAGAITDIVEVLKNG-SMEARENAAATLFSL 490
Query: 422 TMVDEFKITIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVSGAVPL 481
+++DE K+ IGA+ AI AL+ LL+EG GK+DAATA+ NL +Y NK+ V G V
Sbjct: 491 SVIDENKVAIGAA-GAIQALISLLEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGGIVDP 550
Query: 482 LIELLTDDKAGITDDALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDSSLT 541
L LL D G+ D+AL L+++ EG I ++ + +L++++R GSP+ ++++
Sbjct: 551 LTRLLKDAGGGMVDEALAILAILSTNQEGKTAIAEAE-SIPVLVEIIRTGSPRNRENAAA 610
Query: 542 LLLGLCKDGGE--EVARRLLINPRSIPSLQSLAADGSLKARRKADALLRLLNR 581
+L LC E VAR + + +L+ L +G+ +A+RKA +LL L+ +
Sbjct: 611 ILWYLCIGNIERLNVAREV----GADVALKELTENGTDRAKRKAASLLELIQQ 622
HSP 2 Score: 51.2 bits (121), Expect = 2.5e-06
Identity = 33/102 (32.35%), Postives = 51/102 (50.00%), Query Frame = 1
Query: 53 RRIKLLAFLFEEVQESNSPLPPSSILCLTEVFSVIRRVKILTQSCEEGSCLWSLLQTESI 112
RRI LL+ FEE+ + N L I + + L +S GS L+ L +S+
Sbjct: 42 RRITLLSPFFEELIDVNVELKKDQITGFEAMRIALDSSLELFRSVNGGSKLFQLFDRDSL 101
Query: 113 SNQFYQFVKEIGRVLDILPLSLLKLTDDTREQVELLHSQAKR 155
+F EI L +P ++++++ REQV+LLH Q KR
Sbjct: 102 VEKFRDMTVEIEAALSQIPYEKIEVSEEVREQVQLLHFQFKR 143
BLAST of Cucsa.136750 vs. TAIR10
Match:
AT1G29340.1 (AT1G29340.1 plant U-box 17)
HSP 1 Score: 287.0 bits (733), Expect = 2.7e-77
Identity = 171/396 (43.18%), Postives = 245/396 (61.87%), Query Frame = 1
Query: 186 DFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALRSL 245
DF CPISLD M DPVIIS+G TYDR SIA+WI+ GH CPK+ Q L+ ++PN AL++L
Sbjct: 308 DFVCPISLDLMTDPVIISTGQTYDRNSIARWIEEGHCTCPKTGQMLMDSRIVPNRALKNL 367
Query: 246 MQQWCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVDHISASKAASDAIKMTAEFLVG 305
+ QWC + I+ Y S E S ++ + S P +KAA +A K T L+
Sbjct: 368 IVQWCTASGIS-------YES-EFTDSPNESFASALP------TKAAVEANKATVSILIK 427
Query: 306 KLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLVTLLKSGDPRIEENAVTALF 365
LA GS Q AA E+RLLAKTG +NR IAEAGAIP L LL S + +EN+VTA+
Sbjct: 428 YLADGSQAAQTVAAREIRLLAKTGKENRAYIAEAGAIPHLCRLLTSENAIAQENSVTAML 487
Query: 366 NLAIFNNNKILIVAAG-AIDNITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPK 425
NL+I+ NK I+ G +++I +L SG T+EA+ENAAAT++SL+ V E+K I +
Sbjct: 488 NLSIYEKNKSRIMEEGDCLESIVSVLVSGLTVEAQENAAATLFSLSAVHEYKKRIAIVDQ 547
Query: 426 AIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVSGAVPLLIELLTDDKAGITDD 485
+ AL LL+ G GK+DA TAL NL+ + N + ++ G V L+ L ++ G+ ++
Sbjct: 548 CVEALALLLQNGTPRGKKDAVTALYNLSTHPDNCSRMIEGGGVSSLVGALKNE--GVAEE 607
Query: 486 ALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEVAR 545
A AL+L++ S G + I K V+ L+ ++R G+P+GK++++ LL LC+ GG VA
Sbjct: 608 AAGALALLVRQSLGAEAIGKEDSAVAGLMGMMRCGTPRGKENAVAALLELCRSGGAAVAE 667
Query: 546 RLLINPRSIPSLQSLAADGSLKARRKADALLRLLNR 581
++L P LQ+L G+ +ARRKA +L R+ R
Sbjct: 668 KVLRAPAIAGLLQTLLFTGTKRARRKAASLARVFQR 687
HSP 2 Score: 84.7 bits (208), Expect = 2.0e-16
Identity = 53/175 (30.29%), Postives = 90/175 (51.43%), Query Frame = 1
Query: 22 SLLESLILLSNEVA--FEEKAPFVHAKTISTMRRRIKLLAFLFEEVQESN---------- 81
+L+++L +S+EV F K ++ R+I++ LFE + +SN
Sbjct: 31 ALVQTLASISSEVVSCFTSVRFSFQRKNARSLIRKIEIFVVLFEFLVDSNWGSTTTRTRA 90
Query: 82 ------SPLPPSSILCLTEVFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFVKEI 141
S +++LCL E++ ++ R KIL C + S LW LLQ SIS F+ +EI
Sbjct: 91 RRRSKSSVSESTALLCLKELYLLLYRSKILVDYCAQSSKLWLLLQNPSISGYFHDLNQEI 150
Query: 142 GRVLDILPLSLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDELLQLMSNKE 179
+LD+LP++ L L+DD REQ+ELL Q+++ ++ + R+ + E
Sbjct: 151 STLLDVLPVNDLGLSDDIREQIELLQRQSRKARLYIDKNDESLRESFYSFLDGFE 205
BLAST of Cucsa.136750 vs. TAIR10
Match:
AT2G28830.1 (AT2G28830.1 PLANT U-BOX 12)
HSP 1 Score: 285.0 bits (728), Expect = 1.0e-76
Identity = 184/462 (39.83%), Postives = 269/462 (58.23%), Query Frame = 1
Query: 139 DDTREQVELLHSQAKRFEFSVNAREVQRRDELLQLMSNKERNYKTKDD----------FR 198
DD E E + K+ + V D L+L S+ K++DD FR
Sbjct: 205 DDPGESFEKMSMVLKKIKDFVQTYNPNLDDAPLRLKSSLP---KSRDDDRDMLIPPEEFR 264
Query: 199 CPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALRSLMQQ 258
CPISL+ M DPVI+SSG TY+R I +W++ GH CPK+ + L + PNY LRSL+ Q
Sbjct: 265 CPISLELMTDPVIVSSGQTYERECIKKWLEGGHLTCPKTQETLTSDIMTPNYVLRSLIAQ 324
Query: 259 WCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVDHISASKAASDAIKMTAEFLVGKLA 318
WC+ N I P +P S +P S+S +A D E L+ KL
Sbjct: 325 WCESNGIE--PPKRPNIS--------------QPSSKASSSSSAPDDEHNKIEELLLKLT 384
Query: 319 TGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLVTLLK-SGDPRIEENAVTALFNL 378
+ P+ +R AA E+RLLAK NR IA +GAIP LV LL S D R +E+AVT++ NL
Sbjct: 385 SQQPEDRRSAAGEIRLLAKQNNHNRVAIAASGAIPLLVNLLTISNDSRTQEHAVTSILNL 444
Query: 379 AIFNNNKILIV-AAGAIDNITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPKAI 438
+I NK IV ++GA+ I H+L+ G +MEARENAAAT++SL+++DE K+TIGA+ AI
Sbjct: 445 SICQENKGKIVYSSGAVPGIVHVLQKG-SMEARENAAATLFSLSVIDENKVTIGAA-GAI 504
Query: 439 PALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVSGAVPLLIELLTDDKAGITDDAL 498
P LV LL EG+ GK+DAATAL NL ++ NK V +G VP+L+ LLT+ ++G+ D++L
Sbjct: 505 PPLVTLLSEGSQRGKKDAATALFNLCIFQGNKGKAVRAGLVPVLMRLLTEPESGMVDESL 564
Query: 499 QALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEV--AR 558
L+++ +G E+ + V +L+D +R GSP+ K++S +L+ LC + + A+
Sbjct: 565 SILAILSSHPDGKSEVGAADA-VPVLVDFIRSGSPRNKENSAAVLVHLCSWNQQHLIEAQ 624
Query: 559 RLLINPRSIPSLQSLAADGSLKARRKADALLRLLNRCCSQSQ 587
+L I + L +A +G+ + +RKA LL +R Q +
Sbjct: 625 KLGI----MDLLIEMAENGTDRGKRKAAQLLNRFSRFNDQQK 640
HSP 2 Score: 46.6 bits (109), Expect = 6.2e-05
Identity = 33/132 (25.00%), Postives = 64/132 (48.48%), Query Frame = 1
Query: 23 LLESLILLSNEVAFEEKAPFVHAKTISTMRRRIKLLAFLFEEVQESNSPLPPSSILCLTE 82
L ++LI NE+A + K + + RR+ LL + EE+++ N + L
Sbjct: 9 LAQTLIDSINEIASISDSVTPMKKHCANLSRRLSLLLPMLEEIRD-NQESSSEVVNALLS 68
Query: 83 VFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFVKEIGRVLDILPLSLLKLTDDTR 142
V + K L S ++ +L+ + + +F + + + L I+P L+++D+ +
Sbjct: 69 VKQSLLHAKDLLSFVSHVSKIYLVLERDQVMVKFQKVTSLLEQALSIIPYENLEISDELK 128
Query: 143 EQVELLHSQAKR 155
EQVEL+ Q +R
Sbjct: 129 EQVELVLVQLRR 139
BLAST of Cucsa.136750 vs. TAIR10
Match:
AT1G23030.1 (AT1G23030.1 ARM repeat superfamily protein)
HSP 1 Score: 275.0 bits (702), Expect = 1.1e-73
Identity = 165/399 (41.35%), Postives = 245/399 (61.40%), Query Frame = 1
Query: 186 DFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALRSL 245
DF CP+SL+ M+DPVI+++G TY+R I +WID G+ CPK+ Q+L + L PNY LRSL
Sbjct: 244 DFLCPVSLELMKDPVIVATGQTYERAYIQRWIDCGNLTCPKTQQKLENFTLTPNYVLRSL 303
Query: 246 MQQWCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVDHISA-SKAASDAIKMTAEFLV 305
+ +WC E+NI E+P +I+ +K + D + A LV
Sbjct: 304 ISRWCAEHNI------------------------EQPAGYINGRTKNSGDMSVIRA--LV 363
Query: 306 GKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLVTLLKSGDPRIEENAVTAL 365
+L++ S + +R A E+R L+K DNR +IAEAGAIP LV LL S D +ENA+T +
Sbjct: 364 QRLSSRSTEDRRNAVSEIRSLSKRSTDNRILIAEAGAIPVLVNLLTSEDVATQENAITCV 423
Query: 366 FNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPK 425
NL+I+ NNK LI+ AGA+ +I +L +G TMEARENAAAT++SL++ DE KI IG S
Sbjct: 424 LNLSIYENNKELIMFAGAVTSIVQVLRAG-TMEARENAAATLFSLSLADENKIIIGGS-G 483
Query: 426 AIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVSGAVPLLIELLTDD-KAGITD 485
AIPALV LL+ G GK+DAATAL NL +Y+ NK V +G V L+++L+D + + D
Sbjct: 484 AIPALVDLLENGTPRGKKDAATALFNLCIYHGNKGRAVRAGIVTALVKMLSDSTRHRMVD 543
Query: 486 DALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEVA 545
+AL LS++ + I K+ L + LI +L+ + ++++ +LL LCK E++
Sbjct: 544 EALTILSVLANNQDAKSAIVKANTLPA-LIGILQTDQTRNRENAAAILLSLCKRDTEKLI 603
Query: 546 RRLLINPRSIPSLQSLAADGSLKARRKADALLRLLNRCC 583
+ ++ L L+ +G+ + +RKA +LL LL + C
Sbjct: 604 --TIGRLGAVVPLMDLSKNGTERGKRKAISLLELLRKAC 611
HSP 2 Score: 36.2 bits (82), Expect = 8.3e-02
Identity = 31/120 (25.83%), Postives = 54/120 (45.00%), Query Frame = 1
Query: 46 KTISTMRRRIKLLAFLFEEVQES---NSPLPPSS--------ILCLTEVFSVIRRVKILT 105
K + + RR+ LL L EE+++S +S SS ++ L ++ +
Sbjct: 30 KDCADLTRRVCLLTHLLEEIRDSTPIDSAASSSSENDWWSDLVVGLQAAKRLLSTARFQA 89
Query: 106 QSCEEGSCLWSLLQTESISNQFYQFVKEIGRVLDILPLSLLKLTDDTREQVELLHSQAKR 155
+ +G+ + IS QF ++ + L LP L ++D+ EQVEL SQ +R
Sbjct: 90 RDSSDGAA-------KRISFQFQCVTWKLEKALSNLPYDLYDISDEVGEQVELARSQLRR 142
BLAST of Cucsa.136750 vs. NCBI nr
Match:
gi|778709556|ref|XP_004138560.2| (PREDICTED: U-box domain-containing protein 13-like [Cucumis sativus])
HSP 1 Score: 788.1 bits (2034), Expect = 1.1e-224
Identity = 404/404 (100.00%), Postives = 404/404 (100.00%), Query Frame = 1
Query: 185 DDFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALRS 244
DDFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALRS
Sbjct: 285 DDFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALRS 344
Query: 245 LMQQWCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVDHISASKAASDAIKMTAEFLV 304
LMQQWCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVDHISASKAASDAIKMTAEFLV
Sbjct: 345 LMQQWCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVDHISASKAASDAIKMTAEFLV 404
Query: 305 GKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLVTLLKSGDPRIEENAVTAL 364
GKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLVTLLKSGDPRIEENAVTAL
Sbjct: 405 GKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLVTLLKSGDPRIEENAVTAL 464
Query: 365 FNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPK 424
FNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPK
Sbjct: 465 FNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPK 524
Query: 425 AIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVSGAVPLLIELLTDDKAGITDD 484
AIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVSGAVPLLIELLTDDKAGITDD
Sbjct: 525 AIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVSGAVPLLIELLTDDKAGITDD 584
Query: 485 ALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEVAR 544
ALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEVAR
Sbjct: 585 ALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEVAR 644
Query: 545 RLLINPRSIPSLQSLAADGSLKARRKADALLRLLNRCCSQSQPC 589
RLLINPRSIPSLQSLAADGSLKARRKADALLRLLNRCCSQSQPC
Sbjct: 645 RLLINPRSIPSLQSLAADGSLKARRKADALLRLLNRCCSQSQPC 688
BLAST of Cucsa.136750 vs. NCBI nr
Match:
gi|778709556|ref|XP_004138560.2| (PREDICTED: U-box domain-containing protein 13-like [Cucumis sativus])
HSP 1 Score: 351.7 bits (901), Expect = 2.5e-93
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 1
Query: 1 MAMDVALSSMMAASSSLFPSGSLLESLILLSNEVAFEEKAPFVHAKTISTMRRRIKLLAF 60
MAMDVALSSMMAASSSLFPSGSLLESLILLSNEVAFEEKAPFVHAKTISTMRRRIKLLAF
Sbjct: 1 MAMDVALSSMMAASSSLFPSGSLLESLILLSNEVAFEEKAPFVHAKTISTMRRRIKLLAF 60
Query: 61 LFEEVQESNSPLPPSSILCLTEVFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFV 120
LFEEVQESNSPLPPSSILCLTEVFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFV
Sbjct: 61 LFEEVQESNSPLPPSSILCLTEVFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFV 120
Query: 121 KEIGRVLDILPLSLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDELLQLMSNKERN 180
KEIGRVLDILPLSLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDELLQLMSNKERN
Sbjct: 121 KEIGRVLDILPLSLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDELLQLMSNKERN 180
Query: 181 YKTK 185
YKTK
Sbjct: 181 YKTK 184
HSP 2 Score: 780.0 bits (2013), Expect = 2.9e-222
Identity = 401/424 (94.58%), Postives = 409/424 (96.46%), Query Frame = 1
Query: 165 QRRDELLQLMSNKERNYKTKDDFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVC 224
Q ++ L S+ + DDFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVC
Sbjct: 265 QHSNKHLDHSSSSNSLVQIPDDFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVC 324
Query: 225 PKSNQRLIHMALIPNYALRSLMQQWCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVD 284
PKSNQRLIHMALIPNYAL+SLMQQWCQENNINMNEPTKPYSSF LERSNSK+YLSEEPVD
Sbjct: 325 PKSNQRLIHMALIPNYALKSLMQQWCQENNINMNEPTKPYSSFGLERSNSKKYLSEEPVD 384
Query: 285 HISASKAASDAIKMTAEFLVGKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPF 344
HISASKAASDA+KMTAEFLVGKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPF
Sbjct: 385 HISASKAASDAVKMTAEFLVGKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPF 444
Query: 345 LVTLLKSGDPRIEENAVTALFNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAA 404
LVTLLKSGDPRIEENAVTALFNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAA
Sbjct: 445 LVTLLKSGDPRIEENAVTALFNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAA 504
Query: 405 TIYSLTMVDEFKITIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVS 464
TIYSLTMVDEFKITIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVV+
Sbjct: 505 TIYSLTMVDEFKITIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVA 564
Query: 465 GAVPLLIELLTDDKAGITDDALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGK 524
GAVPLLIELLTDDKAGITDDALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGK
Sbjct: 565 GAVPLLIELLTDDKAGITDDALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGK 624
Query: 525 DSSLTLLLGLCKDGGEEVARRLLINPRSIPSLQSLAADGSLKARRKADALLRLLNRCCSQ 584
DSSLTLLLGLCKDGGEEVARRLLINPRSIPSLQSLAADGSLKARRKADALLRLLNRCC Q
Sbjct: 625 DSSLTLLLGLCKDGGEEVARRLLINPRSIPSLQSLAADGSLKARRKADALLRLLNRCCFQ 684
Query: 585 SQPC 589
SQPC
Sbjct: 685 SQPC 688
BLAST of Cucsa.136750 vs. NCBI nr
Match:
gi|659081718|ref|XP_008441478.1| (PREDICTED: U-box domain-containing protein 13-like [Cucumis melo])
HSP 1 Score: 349.0 bits (894), Expect = 1.6e-92
Identity = 181/184 (98.37%), Postives = 183/184 (99.46%), Query Frame = 1
Query: 1 MAMDVALSSMMAASSSLFPSGSLLESLILLSNEVAFEEKAPFVHAKTISTMRRRIKLLAF 60
MAMDVALSSMMAASSSLFPSGSLLESLILLSNEVAFEEKAPF+HAKTISTMRRRIKLLAF
Sbjct: 1 MAMDVALSSMMAASSSLFPSGSLLESLILLSNEVAFEEKAPFIHAKTISTMRRRIKLLAF 60
Query: 61 LFEEVQESNSPLPPSSILCLTEVFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFV 120
LFEEVQESNSPLPPSSILCLTE+FSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFV
Sbjct: 61 LFEEVQESNSPLPPSSILCLTEIFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFV 120
Query: 121 KEIGRVLDILPLSLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDELLQLMSNKERN 180
KEIGRVLDILPLSLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDELLQLMSNKERN
Sbjct: 121 KEIGRVLDILPLSLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDELLQLMSNKERN 180
Query: 181 YKTK 185
YK K
Sbjct: 181 YKNK 184
HSP 2 Score: 702.6 bits (1812), Expect = 5.9e-199
Identity = 373/598 (62.37%), Postives = 459/598 (76.76%), Query Frame = 1
Query: 3 MDVALSSMMAASSSLFPSGSLLESLILLSNEVAFEEKAPFVHAKTISTMRRRIKLLAFLF 62
M+ + S M S L P+ SLL+SL+L++NE+ EK P VH +S+M RRIKLL+ LF
Sbjct: 1 MNDSRSKNMMISPGLLPTESLLDSLVLITNEIGSIEKLPLVHKSNVSSMIRRIKLLSSLF 60
Query: 63 EEVQESNSPLPPSSILCLTEVFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFVKE 122
EE+QES + LPPSSILC TE+ SVI RVK+L Q C++GS LWSL+Q +SIS+QFY VKE
Sbjct: 61 EEIQESETQLPPSSILCFTELLSVITRVKVLIQECKDGSSLWSLMQLDSISSQFYVLVKE 120
Query: 123 IGRVLDILPLSLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDELLQLMSNKERNYK 182
+GR LDILPL+LL +T D +EQ+ELL+ QAKR E ++A+E QRR+ +L++MSN N +
Sbjct: 121 MGRALDILPLNLLNVTFDIKEQIELLNKQAKRGELFIDAKEFQRREMILEVMSNN--NVQ 180
Query: 183 TKDDFRCPISLDFMRDPVIISS------GHTYDRYSI----------AQWIDSGHHVCPK 242
K + +DF++ I+SS D S +WI+SGHH CPK
Sbjct: 181 NKKNSNNKGFIDFVKVEEIMSSIGLRNLSDYVDEISKLEVEAQNQAGTEWINSGHHTCPK 240
Query: 243 SNQRLIHMALIPNYALRSLMQQWCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVDHI 302
S QRLIH ALIPNYAL+SL+ QWC ENN+ +N+ ++ + + N +DHI
Sbjct: 241 SGQRLIHTALIPNYALKSLVHQWCYENNVTLNDSISNVNNNKRNKKNE-----HAAIDHI 300
Query: 303 SASKAASDAIKMTAEFLVGKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLV 362
SA+KAA+DA+KMTAEFLVGKLATGS DIQRQ+AYE+R+LAKTGMDNRR+IAE GAIPFLV
Sbjct: 301 SANKAATDAVKMTAEFLVGKLATGSTDIQRQSAYEIRILAKTGMDNRRIIAEVGAIPFLV 360
Query: 363 TLLKSGDPRIEENAVTALFNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAATI 422
TLL S D RI+E+ VTALFNL+I++NNKILI+AAGA++NI +LESGKTMEARENAAA I
Sbjct: 361 TLLASKDSRIQEHVVTALFNLSIYDNNKILIMAAGAVENIVKVLESGKTMEARENAAAAI 420
Query: 423 YSLTMVDEFKITIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVSGA 482
YSL+MVD+ K+ IG S AIPAL+ LLKEG GKRDAATAL NLA+YN NK IV SG
Sbjct: 421 YSLSMVDDCKVQIGGSTNAIPALIGLLKEGTQIGKRDAATALFNLAVYNPNKISIVKSGG 480
Query: 483 VPLLIELLTDDKAGITDDALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDS 542
V LL+ELL DDKAGITDD+L L+++LGCS+GL+EI+ SR LV LLIDLLRFGS KGK++
Sbjct: 481 VSLLVELLMDDKAGITDDSLAVLAVLLGCSQGLEEIKNSRGLVPLLIDLLRFGSVKGKEN 540
Query: 543 SLTLLLGLCKDGGEEVARRLLINPRSIPSLQSLAADGSLKARRKADALLRLLNRCCSQ 585
S+TLLLGLCK+ GE VAR+LL NPRSIPSLQSLAADGSL+ARRKADALLRLLNRCCSQ
Sbjct: 541 SITLLLGLCKEEGELVARKLLANPRSIPSLQSLAADGSLRARRKADALLRLLNRCCSQ 591
BLAST of Cucsa.136750 vs. NCBI nr
Match:
gi|459680682|gb|AGG82490.1| (E3 ubiquitin ligase [Datisca glomerata])
HSP 1 Score: 656.0 bits (1691), Expect = 6.3e-185
Identity = 339/442 (76.70%), Postives = 385/442 (87.10%), Query Frame = 1
Query: 147 LLHSQAKRFEFSVNAREV--QRRDELLQLMSNKERNYKTKDDFRCPISLDFMRDPVIISS 206
+L+S++ F + + + V Q ++ S+ + D+FRCPISLD M+DPVII+S
Sbjct: 230 VLYSKSMLFSYGDDDKSVKDQSSNKRFDHSSSSKSLVPIPDEFRCPISLDLMKDPVIIAS 289
Query: 207 GHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALRSLMQQWCQENNINMNEPTKPY 266
G TYDR SIAQWIDSGHHVCPKS QRLIHMALIPNYAL+SL+QQWCQENN+ +NEP
Sbjct: 290 GQTYDRTSIAQWIDSGHHVCPKSGQRLIHMALIPNYALKSLVQQWCQENNVQLNEPAT-- 349
Query: 267 SSFELERSNSKRYLSEEPVDHISASKAASDAIKMTAEFLVGKLATGSPDIQRQAAYELRL 326
+S ELERSNS+R LSE VDHIS +KAA DA+KMTAEFLVGKLATGS DIQRQAAYE+RL
Sbjct: 350 ASSELERSNSRRKLSENAVDHISVAKAAIDAVKMTAEFLVGKLATGSSDIQRQAAYEIRL 409
Query: 327 LAKTGMDNRRMIAEAGAIPFLVTLLKSGDPRIEENAVTALFNLAIFNNNKILIVAAGAID 386
LAKTGMDNRRMIAEAGAIPFLVTLL S DPR++ENAVTAL NL+IFNNNK LI++AGAID
Sbjct: 410 LAKTGMDNRRMIAEAGAIPFLVTLLTSTDPRLQENAVTALLNLSIFNNNKTLIISAGAID 469
Query: 387 NITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPKAIPALVRLLKEGNSAGKRDA 446
NI H+LESGKT EARENAAA I+SL+MVDE+K+ IGA P+AIP LVRLL+EG SAGKRDA
Sbjct: 470 NIIHVLESGKTTEARENAAAAIFSLSMVDEYKVVIGAHPRAIPGLVRLLREGTSAGKRDA 529
Query: 447 ATALCNLALYNANKACIVVSGAVPLLIELLTDDKAGITDDALQALSLVLGCSEGLQEIRK 506
A AL NL++Y+ANKA +VV+GAVPLLI+LLTDDKAGITDDALQ LSLVLGCS+GLQEIRK
Sbjct: 530 AIALFNLSIYSANKARVVVAGAVPLLIDLLTDDKAGITDDALQVLSLVLGCSDGLQEIRK 589
Query: 507 SRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEVARRLLINPRSIPSLQSLAADGS 566
SRVLV LLIDLLRFGS KGK++S+TLLLGLCKDGGEEVARRLL+NPRSIPSLQSLAADGS
Sbjct: 590 SRVLVPLLIDLLRFGSLKGKENSITLLLGLCKDGGEEVARRLLMNPRSIPSLQSLAADGS 649
Query: 567 LKARRKADALLRLLNRCCSQSQ 587
LKARRKADALLRLLNRCCS SQ
Sbjct: 650 LKARRKADALLRLLNRCCSHSQ 669
BLAST of Cucsa.136750 vs. NCBI nr
Match:
gi|459680682|gb|AGG82490.1| (E3 ubiquitin ligase [Datisca glomerata])
HSP 1 Score: 231.1 bits (588), Expect = 5.0e-57
Identity = 119/173 (68.79%), Postives = 143/173 (82.66%), Query Frame = 1
Query: 13 ASSSLFPSGSLLESLILLSNEVAFEEKAPFVHAKTISTMRRRIKLLAFLFEEVQESNSPL 72
ASS P+GSLLESLI +SNEVA E PFV + ISTMRRRIKLL+FLFEE+QE+NSPL
Sbjct: 2 ASSGFLPTGSLLESLIHISNEVASMENMPFVQVRNISTMRRRIKLLSFLFEEIQEANSPL 61
Query: 73 PPSSILCLTEVFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFVKEIGRVLDILPL 132
PPSSILC TE+FSVIRRVK L Q C+EGS LW+LLQTE I+NQFY VKE+ R LDILPL
Sbjct: 62 PPSSILCFTELFSVIRRVKFLIQGCKEGSSLWNLLQTEFITNQFYVLVKEMVRALDILPL 121
Query: 133 SLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDELLQLMS-NKERNYKTK 185
SLLK+T D REQVELLH+QAK+ E SV+ +++QRR+EL+Q+M+ N E+N + K
Sbjct: 122 SLLKITADVREQVELLHNQAKQVELSVDPQDLQRREELVQVMTKNNEKNSRNK 174
HSP 2 Score: 626.7 bits (1615), Expect = 4.1e-176
Identity = 315/411 (76.64%), Postives = 366/411 (89.05%), Query Frame = 1
Query: 175 SNKERNYKTKDDFRCPISLDFMRDPVIISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHM 234
S+ + ++ D++RCPISLD MRDPVI++SGHTYDR SIAQWI++GHH CPKS QRLIHM
Sbjct: 271 SSSQSSFNIPDEYRCPISLDLMRDPVIVASGHTYDRNSIAQWINTGHHTCPKSGQRLIHM 330
Query: 235 ALIPNYALRSLMQQWCQENNINMNEPTKPYSSFELERSNSKRYLSEEPVDHISASKAASD 294
ALIPNYAL+SL+ QWC+ENNI + E T S +L+RS+ KR L ++ VDHISA+K ASD
Sbjct: 331 ALIPNYALKSLIHQWCEENNITIRESTS--SPPDLDRSSGKRELQDKAVDHISATKTASD 390
Query: 295 AIKMTAEFLVGKLATGSPDIQRQAAYELRLLAKTGMDNRRMIAEAGAIPFLVTLLKSGDP 354
A+KMTAEFLVGKLATGSPDIQRQAAYELRLLAKTGM+NRRMIAEAGAIPFLVTLL S D
Sbjct: 391 AVKMTAEFLVGKLATGSPDIQRQAAYELRLLAKTGMENRRMIAEAGAIPFLVTLLGSPDS 450
Query: 355 RIEENAVTALFNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAATIYSLTMVDE 414
RI+ NAVTAL NL+IF+NNKILI++AG++D+I H+L SGKTMEARENAAATI+SL+MVD+
Sbjct: 451 RIQGNAVTALLNLSIFDNNKILIMSAGSLDSIIHVLLSGKTMEARENAAATIFSLSMVDD 510
Query: 415 FKITIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYNANKACIVVSGAVPLLIELL 474
+K+ IG PKAIPALV LL +G +AGKRDAATAL NLA+YN NK+C+ VSGA+PLLIELL
Sbjct: 511 YKVLIGGHPKAIPALVELLIDGTTAGKRDAATALFNLAVYNINKSCVAVSGAIPLLIELL 570
Query: 475 TDDKAGITDDALQALSLVLGCSEGLQEIRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGL 534
DDKAGITDDAL L+L+LGCSEGL+EI+KSRVLV LLIDLLR+GSPKGK++S+TLLLGL
Sbjct: 571 MDDKAGITDDALAVLALLLGCSEGLEEIKKSRVLVPLLIDLLRYGSPKGKENSITLLLGL 630
Query: 535 CKDGGEEVARRLLINPRSIPSLQSLAADGSLKARRKADALLRLLNRCCSQS 586
CKD GEEVARRLL+NPRSIPSLQSLAADGSLKARRKADALLRLLNRCC QS
Sbjct: 631 CKDSGEEVARRLLMNPRSIPSLQSLAADGSLKARRKADALLRLLNRCCFQS 679
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| PUB1_MEDTR | 4.2e-165 | 74.50 | U-box domain-containing protein 1 OS=Medicago truncatula GN=PUB1 PE=1 SV=1 | [more] |
| PUB13_ARATH | 1.1e-83 | 46.17 | U-box domain-containing protein 13 OS=Arabidopsis thaliana GN=PUB13 PE=1 SV=1 | [more] |
| PUB12_ORYSJ | 1.2e-79 | 42.09 | U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica GN=PUB12 PE=2... | [more] |
| PUB14_ARATH | 2.1e-79 | 35.24 | U-box domain-containing protein 14 OS=Arabidopsis thaliana GN=PUB14 PE=1 SV=1 | [more] |
| SL11_ORYSJ | 6.7e-78 | 42.54 | E3 ubiquitin-protein ligase SPL11 OS=Oryza sativa subsp. japonica GN=SPL11 PE=1 ... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0K7R8_CUCSA | 7.4e-225 | 100.00 | Uncharacterized protein OS=Cucumis sativus GN=Csa_6G012250 PE=4 SV=1 | [more] |
| A0A0A0K7R8_CUCSA | 1.8e-93 | 100.00 | Uncharacterized protein OS=Cucumis sativus GN=Csa_6G012250 PE=4 SV=1 | [more] |
| T1R384_DATGL | 3.5e-57 | 68.79 | E3 ubiquitin ligase OS=Datisca glomerata GN=PUB1 PE=2 SV=1 | [more] |
| W9RRI9_9ROSA | 6.1e-54 | 59.90 | U-box domain-containing protein 13 OS=Morus notabilis GN=L484_009898 PE=4 SV=1 | [more] |
| F6HCB0_VITVI | 2.8e-54 | 64.02 | Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0067g01460 PE=4 SV=... | [more] |
