Carg05967 (gene) Silver-seed gourd

NameCarg05967
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionRING-type E3 ubiquitin transferase
LocationCucurbita_argyrosperma_scaffold_026 : 466974 .. 468965 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGTTGCTCTTTCCTCCCCAATCATGGCAGCTTCAGCCTTTTTGCCTCATGGGTCTGTGCTGCAATCACTGATTGTGTTAGCCAACGAAGTTTGTTTGGAGGAGAAGGCGAGTTTTGTGCAGGCTCGGACCGTCTCAACCATGAGAAGAAGAATCAAGCTTCTTGCTTTCTTATTTGAAGAGGTTCATGAATCTAATTCTCCTCAGCTTCCTCCATCTTCCCTTGTTTGCTTCACTGAACTCTTCTCTGTAGTCCGAAGAGTTAAGCTTCTGATTCAAAGCTGTGAGGAAGGAAGCTGTCTATGGAGTTTACTACAGACAGAGCTCATTTCCAATCAGTTCTATCAGCTAGTGAAGGAGATTGGGAGAGTTCTTGATATTTTACCTCTCAGCTTCCTGAAATTAACAGATGATACAAGAGAACAAGTGGAGCTTCTTCACAATCAAGCTAAACGATTCGAGTTTTCGGCCGACGAACGCGAGGTTCGAAGAAGAGATGAGCTTCTTGAACTAATGATGAACAAGAAGAACAAAGATTTGGCAGAGTTTTTCAGTAGTGTTGGGCTGAGAAGCCTGATGGATTGTGATGAAGAGATATCAAAACTGGAAGTAGAAGCTCTTAAACAAGCAGGAACAGGTGGAATAATGGTGGTTTCTAATATAAACAATCTAATTTCTCTTGTTCGACATGCCAAAACAGTGCTTTTCAGTAAGACAGAACAATTCAACTTGAAGTTTCAACATCATTCGTGTTCTTCTATATCAATGGATCAAATTCCAGATGATTTTCGCTGCCCCATTTCGTTAGATGTGATGAGAGATCCTGTAATTGTATCATCAGGACATACCTATGATCGTAATTCAATAGCTCAATGGATTGACTCAGGGCATCATGTATGCCCCAAGAGTAATCAGAGGCTAATTCATGTGGCTCTCATACCAAATTATGCACTCAAAAGTCTGATGCAACAATGGTGCCAAGAAAACAACGTTAATTTGTTTGAACCTACAAAACCATGTTCTTTCTTAAAGAGAAGCAATAGCAGACGATACCGTTCCGAGGAACCGATCGATCAAATTTCGGCGTCGAAAGCCGCCTCCGACGCTGTGAAAATGACAGCGGAATTTCTGGTGGGGAAGCTTGCAACCGGATCACCAGATGTTCAAAGACAAGCAGCATATGAGCTCCGGTTGCTAGCAAAAACAGGGATGGATAGCCGAAGGATGATTGCGGAGGCAGGAGCAATACCGTTTCTTGTGACATTGTTGAGATCTGGTGATCCAAGGATTGAGGAAAATGCAGTGACGGCATTGTTCAACTTGGCAATCTTTAACAACAACAAGATCCTGATAGTGGCAGCAGGGGCACTAGACAACATAACACACATCTTAGAATCAGGGAAAACAATGGAAGCAAGAGAAAACGCTGCAGCAACAATATTTAGCTTAACAATGGTGGATGAGTTCAAAATATCGATAGGGGCGAGTCCGAAAGCGATACCGGCACTGGTGAGGCTCTTGAAAGAAGGCAATTCAGCCGGGAAGCGCGACGCCGCCACTGCCCTTTGCAATCTAGCGCTTTACAAAGCCAACAAAGCTTGCATTGTAGGTGCTGGGGCAGTGCCATTGCTGATTGAGTTGCTGACAGATGAGAAGGCTGGCATAACAGATGATGCGTTGCAGGCGCTGTCTTTGGTTTTGGGCTGCTCGGAAGGGCTGCAGGAGATAAGGAAGAGCAGAGTTTTGGTGCCTTTGCTGGTTGATCTTTTAAGATTTGGATCTCCAAATGGAAAAGAGAGTTCAATTACGGTGTTGTTGGGGATGTGCAAAGATGGAGGAGAGGAGGTTGGGAGGCGGTTGTTGATGAATCCTCGGAGTATCCCTTCGCTGCAAAGCTTGGCAGCTGATGGATCATTGAAGGCCAGAAGAAAGGCTGATGCTTTGCTAAGATTGCTTAATAGATGCTGCTTTCAATCTCAGCCATGTTGA

mRNA sequence

ATGGATGTTGCTCTTTCCTCCCCAATCATGGCAGCTTCAGCCTTTTTGCCTCATGGGTCTGTGCTGCAATCACTGATTGTGTTAGCCAACGAAGTTTGTTTGGAGGAGAAGGCGAGTTTTGTGCAGGCTCGGACCGTCTCAACCATGAGAAGAAGAATCAAGCTTCTTGCTTTCTTATTTGAAGAGGTTCATGAATCTAATTCTCCTCAGCTTCCTCCATCTTCCCTTGTTTGCTTCACTGAACTCTTCTCTGTAGTCCGAAGAGTTAAGCTTCTGATTCAAAGCTGTGAGGAAGGAAGCTGTCTATGGAGTTTACTACAGACAGAGCTCATTTCCAATCAGTTCTATCAGCTAGTGAAGGAGATTGGGAGAGTTCTTGATATTTTACCTCTCAGCTTCCTGAAATTAACAGATGATACAAGAGAACAAGTGGAGCTTCTTCACAATCAAGCTAAACGATTCGAGTTTTCGGCCGACGAACGCGAGGTTCGAAGAAGAGATGAGCTTCTTGAACTAATGATGAACAAGAAGAACAAAGATTTGGCAGAGTTTTTCAGTAGTGTTGGGCTGAGAAGCCTGATGGATTGTGATGAAGAGATATCAAAACTGGAAGTAGAAGCTCTTAAACAAGCAGGAACAGGTGGAATAATGGTGGTTTCTAATATAAACAATCTAATTTCTCTTGTTCGACATGCCAAAACAGTGCTTTTCAGTAAGACAGAACAATTCAACTTGAAGTTTCAACATCATTCGTGTTCTTCTATATCAATGGATCAAATTCCAGATGATTTTCGCTGCCCCATTTCGTTAGATGTGATGAGAGATCCTGTAATTGTATCATCAGGACATACCTATGATCGTAATTCAATAGCTCAATGGATTGACTCAGGGCATCATGTATGCCCCAAGAGTAATCAGAGGCTAATTCATGTGGCTCTCATACCAAATTATGCACTCAAAAGTCTGATGCAACAATGGTGCCAAGAAAACAACGTTAATTTGTTTGAACCTACAAAACCATGTTCTTTCTTAAAGAGAAGCAATAGCAGACGATACCGTTCCGAGGAACCGATCGATCAAATTTCGGCGTCGAAAGCCGCCTCCGACGCTGTGAAAATGACAGCGGAATTTCTGGTGGGGAAGCTTGCAACCGGATCACCAGATGTTCAAAGACAAGCAGCATATGAGCTCCGGTTGCTAGCAAAAACAGGGATGGATAGCCGAAGGATGATTGCGGAGGCAGGAGCAATACCGTTTCTTGTGACATTGTTGAGATCTGGTGATCCAAGGATTGAGGAAAATGCAGTGACGGCATTGTTCAACTTGGCAATCTTTAACAACAACAAGATCCTGATAGTGGCAGCAGGGGCACTAGACAACATAACACACATCTTAGAATCAGGGAAAACAATGGAAGCAAGAGAAAACGCTGCAGCAACAATATTTAGCTTAACAATGGTGGATGAGTTCAAAATATCGATAGGGGCGAGTCCGAAAGCGATACCGGCACTGGTGAGGCTCTTGAAAGAAGGCAATTCAGCCGGGAAGCGCGACGCCGCCACTGCCCTTTGCAATCTAGCGCTTTACAAAGCCAACAAAGCTTGCATTGTAGGTGCTGGGGCAGTGCCATTGCTGATTGAGTTGCTGACAGATGAGAAGGCTGGCATAACAGATGATGCGTTGCAGGCGCTGTCTTTGGTTTTGGGCTGCTCGGAAGGGCTGCAGGAGATAAGGAAGAGCAGAGTTTTGGTGCCTTTGCTGGTTGATCTTTTAAGATTTGGATCTCCAAATGGAAAAGAGAGTTCAATTACGGTGTTGTTGGGGATGTGCAAAGATGGAGGAGAGGAGGTTGGGAGGCGGTTGTTGATGAATCCTCGGAGTATCCCTTCGCTGCAAAGCTTGGCAGCTGATGGATCATTGAAGGCCAGAAGAAAGGCTGATGCTTTGCTAAGATTGCTTAATAGATGCTGCTTTCAATCTCAGCCATGTTGA

Coding sequence (CDS)

ATGGATGTTGCTCTTTCCTCCCCAATCATGGCAGCTTCAGCCTTTTTGCCTCATGGGTCTGTGCTGCAATCACTGATTGTGTTAGCCAACGAAGTTTGTTTGGAGGAGAAGGCGAGTTTTGTGCAGGCTCGGACCGTCTCAACCATGAGAAGAAGAATCAAGCTTCTTGCTTTCTTATTTGAAGAGGTTCATGAATCTAATTCTCCTCAGCTTCCTCCATCTTCCCTTGTTTGCTTCACTGAACTCTTCTCTGTAGTCCGAAGAGTTAAGCTTCTGATTCAAAGCTGTGAGGAAGGAAGCTGTCTATGGAGTTTACTACAGACAGAGCTCATTTCCAATCAGTTCTATCAGCTAGTGAAGGAGATTGGGAGAGTTCTTGATATTTTACCTCTCAGCTTCCTGAAATTAACAGATGATACAAGAGAACAAGTGGAGCTTCTTCACAATCAAGCTAAACGATTCGAGTTTTCGGCCGACGAACGCGAGGTTCGAAGAAGAGATGAGCTTCTTGAACTAATGATGAACAAGAAGAACAAAGATTTGGCAGAGTTTTTCAGTAGTGTTGGGCTGAGAAGCCTGATGGATTGTGATGAAGAGATATCAAAACTGGAAGTAGAAGCTCTTAAACAAGCAGGAACAGGTGGAATAATGGTGGTTTCTAATATAAACAATCTAATTTCTCTTGTTCGACATGCCAAAACAGTGCTTTTCAGTAAGACAGAACAATTCAACTTGAAGTTTCAACATCATTCGTGTTCTTCTATATCAATGGATCAAATTCCAGATGATTTTCGCTGCCCCATTTCGTTAGATGTGATGAGAGATCCTGTAATTGTATCATCAGGACATACCTATGATCGTAATTCAATAGCTCAATGGATTGACTCAGGGCATCATGTATGCCCCAAGAGTAATCAGAGGCTAATTCATGTGGCTCTCATACCAAATTATGCACTCAAAAGTCTGATGCAACAATGGTGCCAAGAAAACAACGTTAATTTGTTTGAACCTACAAAACCATGTTCTTTCTTAAAGAGAAGCAATAGCAGACGATACCGTTCCGAGGAACCGATCGATCAAATTTCGGCGTCGAAAGCCGCCTCCGACGCTGTGAAAATGACAGCGGAATTTCTGGTGGGGAAGCTTGCAACCGGATCACCAGATGTTCAAAGACAAGCAGCATATGAGCTCCGGTTGCTAGCAAAAACAGGGATGGATAGCCGAAGGATGATTGCGGAGGCAGGAGCAATACCGTTTCTTGTGACATTGTTGAGATCTGGTGATCCAAGGATTGAGGAAAATGCAGTGACGGCATTGTTCAACTTGGCAATCTTTAACAACAACAAGATCCTGATAGTGGCAGCAGGGGCACTAGACAACATAACACACATCTTAGAATCAGGGAAAACAATGGAAGCAAGAGAAAACGCTGCAGCAACAATATTTAGCTTAACAATGGTGGATGAGTTCAAAATATCGATAGGGGCGAGTCCGAAAGCGATACCGGCACTGGTGAGGCTCTTGAAAGAAGGCAATTCAGCCGGGAAGCGCGACGCCGCCACTGCCCTTTGCAATCTAGCGCTTTACAAAGCCAACAAAGCTTGCATTGTAGGTGCTGGGGCAGTGCCATTGCTGATTGAGTTGCTGACAGATGAGAAGGCTGGCATAACAGATGATGCGTTGCAGGCGCTGTCTTTGGTTTTGGGCTGCTCGGAAGGGCTGCAGGAGATAAGGAAGAGCAGAGTTTTGGTGCCTTTGCTGGTTGATCTTTTAAGATTTGGATCTCCAAATGGAAAAGAGAGTTCAATTACGGTGTTGTTGGGGATGTGCAAAGATGGAGGAGAGGAGGTTGGGAGGCGGTTGTTGATGAATCCTCGGAGTATCCCTTCGCTGCAAAGCTTGGCAGCTGATGGATCATTGAAGGCCAGAAGAAAGGCTGATGCTTTGCTAAGATTGCTTAATAGATGCTGCTTTCAATCTCAGCCATGTTGA

Protein sequence

MDVALSSPIMAASAFLPHGSVLQSLIVLANEVCLEEKASFVQARTVSTMRRRIKLLAFLFEEVHESNSPQLPPSSLVCFTELFSVVRRVKLLIQSCEEGSCLWSLLQTELISNQFYQLVKEIGRVLDILPLSFLKLTDDTREQVELLHNQAKRFEFSADEREVRRRDELLELMMNKKNKDLAEFFSSVGLRSLMDCDEEISKLEVEALKQAGTGGIMVVSNINNLISLVRHAKTVLFSKTEQFNLKFQHHSCSSISMDQIPDDFRCPISLDVMRDPVIVSSGHTYDRNSIAQWIDSGHHVCPKSNQRLIHVALIPNYALKSLMQQWCQENNVNLFEPTKPCSFLKRSNSRRYRSEEPIDQISASKAASDAVKMTAEFLVGKLATGSPDVQRQAAYELRLLAKTGMDSRRMIAEAGAIPFLVTLLRSGDPRIEENAVTALFNLAIFNNNKILIVAAGALDNITHILESGKTMEARENAAATIFSLTMVDEFKISIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYKANKACIVGAGAVPLLIELLTDEKAGITDDALQALSLVLGCSEGLQEIRKSRVLVPLLVDLLRFGSPNGKESSITVLLGMCKDGGEEVGRRLLMNPRSIPSLQSLAADGSLKARRKADALLRLLNRCCFQSQPC
BLAST of Carg05967 vs. NCBI nr
Match: XP_022963960.1 (U-box domain-containing protein 1-like [Cucurbita moschata])

HSP 1 Score: 1269.2 bits (3283), Expect = 0.0e+00
Identity = 661/663 (99.70%), Postives = 663/663 (100.00%), Query Frame = 0

Query: 1   MDVALSSPIMAASAFLPHGSVLQSLIVLANEVCLEEKASFVQARTVSTMRRRIKLLAFLF 60
           MDVALSSPIMAASAFLPHGSVLQSLIVLANEVCLEEKASFVQARTVSTMRRRIKLLAFLF
Sbjct: 3   MDVALSSPIMAASAFLPHGSVLQSLIVLANEVCLEEKASFVQARTVSTMRRRIKLLAFLF 62

Query: 61  EEVHESNSPQLPPSSLVCFTELFSVVRRVKLLIQSCEEGSCLWSLLQTELISNQFYQLVK 120
           EEVHESNSPQLPPSSLVCFTELFSVVRRVKLLIQSCEEGSCLWSLLQTELISNQFYQLVK
Sbjct: 63  EEVHESNSPQLPPSSLVCFTELFSVVRRVKLLIQSCEEGSCLWSLLQTELISNQFYQLVK 122

Query: 121 EIGRVLDILPLSFLKLTDDTREQVELLHNQAKRFEFSADEREVRRRDELLELMMNKKNKD 180
           EIGRVLDILPLSFLKLTDDTREQVELLHNQAKRFEFSADEREVRRRDELLELMMNKKNKD
Sbjct: 123 EIGRVLDILPLSFLKLTDDTREQVELLHNQAKRFEFSADEREVRRRDELLELMMNKKNKD 182

Query: 181 LAEFFSSVGLRSLMDCDEEISKLEVEALKQAGTGGIMVVSNINNLISLVRHAKTVLFSKT 240
           LAEFFSSVGLRSLMDCDEEISKLEVEALKQAGTGGIMVVSNINNLISLVRHAKTVLFSKT
Sbjct: 183 LAEFFSSVGLRSLMDCDEEISKLEVEALKQAGTGGIMVVSNINNLISLVRHAKTVLFSKT 242

Query: 241 EQFNLKFQHHSCSSISMDQIPDDFRCPISLDVMRDPVIVSSGHTYDRNSIAQWIDSGHHV 300
           EQFNLKFQHHSCSSISMDQIPDDFRCPISLDVMRDPVIVSSGHTYDRNSIAQWIDSGHHV
Sbjct: 243 EQFNLKFQHHSCSSISMDQIPDDFRCPISLDVMRDPVIVSSGHTYDRNSIAQWIDSGHHV 302

Query: 301 CPKSNQRLIHVALIPNYALKSLMQQWCQENNVNLFEPTKPCSFLKRSNSRRYRSEEPIDQ 360
           CPKSNQRLIHVALIPNYALKSLMQQWCQENNVNLFEPTKPCSFLKRSNSRRYRSEEPIDQ
Sbjct: 303 CPKSNQRLIHVALIPNYALKSLMQQWCQENNVNLFEPTKPCSFLKRSNSRRYRSEEPIDQ 362

Query: 361 ISASKAASDAVKMTAEFLVGKLATGSPDVQRQAAYELRLLAKTGMDSRRMIAEAGAIPFL 420
           ISASKAASDAVKMTAEFLVGKLATGSPDVQRQAAYELRLLAKTGMDSRRMIAEAGAIPFL
Sbjct: 363 ISASKAASDAVKMTAEFLVGKLATGSPDVQRQAAYELRLLAKTGMDSRRMIAEAGAIPFL 422

Query: 421 VTLLRSGDPRIEENAVTALFNLAIFNNNKILIVAAGALDNITHILESGKTMEARENAAAT 480
           VTLLRSGDPRIEENAVTALFNLAIFNNNKILIVAAGA+DNITH+LESGKTMEARENAAAT
Sbjct: 423 VTLLRSGDPRIEENAVTALFNLAIFNNNKILIVAAGAIDNITHVLESGKTMEARENAAAT 482

Query: 481 IFSLTMVDEFKISIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYKANKACIVGAG 540
           IFSLTMVDEFKISIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYKANKACIVGAG
Sbjct: 483 IFSLTMVDEFKISIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYKANKACIVGAG 542

Query: 541 AVPLLIELLTDEKAGITDDALQALSLVLGCSEGLQEIRKSRVLVPLLVDLLRFGSPNGKE 600
           AVPLLIELLTDEKAGITDDALQALSLVLGCSEGLQEIRKSRVLVPLLVDLLRFGSPNGKE
Sbjct: 543 AVPLLIELLTDEKAGITDDALQALSLVLGCSEGLQEIRKSRVLVPLLVDLLRFGSPNGKE 602

Query: 601 SSITVLLGMCKDGGEEVGRRLLMNPRSIPSLQSLAADGSLKARRKADALLRLLNRCCFQS 660
           SSITVLLGMCKDGGEEVGRRLLMNPRSIPSLQSLAADGSLKARRKADALLRLLNRCCFQS
Sbjct: 603 SSITVLLGMCKDGGEEVGRRLLMNPRSIPSLQSLAADGSLKARRKADALLRLLNRCCFQS 662

Query: 661 QPC 664
           QPC
Sbjct: 663 QPC 665

BLAST of Carg05967 vs. NCBI nr
Match: XP_022990647.1 (U-box domain-containing protein 1-like [Cucurbita maxima])

HSP 1 Score: 1233.0 bits (3189), Expect = 0.0e+00
Identity = 644/663 (97.13%), Postives = 652/663 (98.34%), Query Frame = 0

Query: 1   MDVALSSPIMAASAFLPHGSVLQSLIVLANEVCLEEKASFVQARTVSTMRRRIKLLAFLF 60
           MDVALSSPIMAASAFLP+GS+LQSLIVLANEVCLEEKASFVQARTVSTMRRRIKLLAFLF
Sbjct: 3   MDVALSSPIMAASAFLPYGSLLQSLIVLANEVCLEEKASFVQARTVSTMRRRIKLLAFLF 62

Query: 61  EEVHESNSPQLPPSSLVCFTELFSVVRRVKLLIQSCEEGSCLWSLLQTELISNQFYQLVK 120
           EEVHESNSPQLPPSSLVCFTELFSVVRRVKLLI SCEEGS LWSLLQTELISNQFYQLVK
Sbjct: 63  EEVHESNSPQLPPSSLVCFTELFSVVRRVKLLILSCEEGSRLWSLLQTELISNQFYQLVK 122

Query: 121 EIGRVLDILPLSFLKLTDDTREQVELLHNQAKRFEFSADEREVRRRDELLELMMNKKNKD 180
           EIGRVLDILPLSFLKLTDDTREQVELLHNQAKRFEFSADERE+RRRDELLELMMNKKNKD
Sbjct: 123 EIGRVLDILPLSFLKLTDDTREQVELLHNQAKRFEFSADERELRRRDELLELMMNKKNKD 182

Query: 181 LAEFFSSVGLRSLMDCDEEISKLEVEALKQAGTGGIMVVSNINNLISLVRHAKTVLFSKT 240
           LAEFFSSVGLRSLMDCDEEISKLEVEALKQAGTGGIMVVSNINNLISLVRHAKTV+FSK 
Sbjct: 183 LAEFFSSVGLRSLMDCDEEISKLEVEALKQAGTGGIMVVSNINNLISLVRHAKTVVFSKK 242

Query: 241 EQFNLKFQHHSCSSISMDQIPDDFRCPISLDVMRDPVIVSSGHTYDRNSIAQWIDSGHHV 300
           EQFNLKFQHHSCSSISMDQIPDDFRCPISLD+MRDPVIVSSGHTYDRNSIAQWIDSGHHV
Sbjct: 243 EQFNLKFQHHSCSSISMDQIPDDFRCPISLDMMRDPVIVSSGHTYDRNSIAQWIDSGHHV 302

Query: 301 CPKSNQRLIHVALIPNYALKSLMQQWCQENNVNLFEPTKPCSFLKRSNSRRYRSEEPIDQ 360
           CPKSNQRLIHVALIPNYALKSLMQQWCQENNVNLFEPTKPCSFLKRSNSRRYRS EPID 
Sbjct: 303 CPKSNQRLIHVALIPNYALKSLMQQWCQENNVNLFEPTKPCSFLKRSNSRRYRSIEPIDH 362

Query: 361 ISASKAASDAVKMTAEFLVGKLATGSPDVQRQAAYELRLLAKTGMDSRRMIAEAGAIPFL 420
           ISASKA +DAVKMTAEFLVGKLATGSPDVQRQAAYELRLLAKTGMDSRRMIAEAGAIPFL
Sbjct: 363 ISASKATTDAVKMTAEFLVGKLATGSPDVQRQAAYELRLLAKTGMDSRRMIAEAGAIPFL 422

Query: 421 VTLLRSGDPRIEENAVTALFNLAIFNNNKILIVAAGALDNITHILESGKTMEARENAAAT 480
           VTLLRSGDPRIEENAVTALFNLAIFNNNKILIVAAGA+DNITHILESGKTM ARENAAAT
Sbjct: 423 VTLLRSGDPRIEENAVTALFNLAIFNNNKILIVAAGAIDNITHILESGKTMGARENAAAT 482

Query: 481 IFSLTMVDEFKISIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYKANKACIVGAG 540
           IFSLTMVDEFKISIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYKANKACIVGAG
Sbjct: 483 IFSLTMVDEFKISIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYKANKACIVGAG 542

Query: 541 AVPLLIELLTDEKAGITDDALQALSLVLGCSEGLQEIRKSRVLVPLLVDLLRFGSPNGKE 600
           AVPLLIELLTDEKAGITDDALQALSLVLGC EGLQEIRKSRVLVPLLVDLLRFGSP GKE
Sbjct: 543 AVPLLIELLTDEKAGITDDALQALSLVLGCCEGLQEIRKSRVLVPLLVDLLRFGSPKGKE 602

Query: 601 SSITVLLGMCKDGGEEVGRRLLMNPRSIPSLQSLAADGSLKARRKADALLRLLNRCCFQS 660
            SITVLLGMCKDGGEEVGRRLLMNPRSIPS+QSLAADGSLKARRKADALLRLLNRCCF S
Sbjct: 603 CSITVLLGMCKDGGEEVGRRLLMNPRSIPSVQSLAADGSLKARRKADALLRLLNRCCFPS 662

Query: 661 QPC 664
           QPC
Sbjct: 663 QPC 665

BLAST of Carg05967 vs. NCBI nr
Match: XP_023535888.1 (U-box domain-containing protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1231.5 bits (3185), Expect = 0.0e+00
Identity = 642/663 (96.83%), Postives = 653/663 (98.49%), Query Frame = 0

Query: 1   MDVALSSPIMAASAFLPHGSVLQSLIVLANEVCLEEKASFVQARTVSTMRRRIKLLAFLF 60
           MDVALSSPIMAASAFLP+GS+LQSLIVLANEVCLEEKASFVQARTVSTMRRRIKLLAFLF
Sbjct: 3   MDVALSSPIMAASAFLPYGSLLQSLIVLANEVCLEEKASFVQARTVSTMRRRIKLLAFLF 62

Query: 61  EEVHESNSPQLPPSSLVCFTELFSVVRRVKLLIQSCEEGSCLWSLLQTELISNQFYQLVK 120
           EEVHESNSPQLPPSSLVCFTELFSV+RRVKLLIQ+CEEGSCLWSLLQTELIS+QFYQLVK
Sbjct: 63  EEVHESNSPQLPPSSLVCFTELFSVIRRVKLLIQTCEEGSCLWSLLQTELISHQFYQLVK 122

Query: 121 EIGRVLDILPLSFLKLTDDTREQVELLHNQAKRFEFSADEREVRRRDELLELMMNKKNKD 180
           EIGRVLDILPLSFLKLTDDTREQVELLHNQAKRFEFSADEREVRRRDELLELMMNKKNKD
Sbjct: 123 EIGRVLDILPLSFLKLTDDTREQVELLHNQAKRFEFSADEREVRRRDELLELMMNKKNKD 182

Query: 181 LAEFFSSVGLRSLMDCDEEISKLEVEALKQAGTGGIMVVSNINNLISLVRHAKTVLFSKT 240
           LAEFFSSVGLRSLMDCDEEISKLEVE  KQAGTGGIMVVSNINNLISLVRHAKTV+FSK 
Sbjct: 183 LAEFFSSVGLRSLMDCDEEISKLEVEGRKQAGTGGIMVVSNINNLISLVRHAKTVVFSKK 242

Query: 241 EQFNLKFQHHSCSSISMDQIPDDFRCPISLDVMRDPVIVSSGHTYDRNSIAQWIDSGHHV 300
           EQFNLKFQHHSCSSISMDQIPDDFRCPISLD+MRDPVIVSSGHTYDRNSIAQWIDSGHHV
Sbjct: 243 EQFNLKFQHHSCSSISMDQIPDDFRCPISLDLMRDPVIVSSGHTYDRNSIAQWIDSGHHV 302

Query: 301 CPKSNQRLIHVALIPNYALKSLMQQWCQENNVNLFEPTKPCSFLKRSNSRRYRSEEPIDQ 360
           CPKSNQRLIHVALIPNYALKSLMQQWC ENNVNL EPTKPCSF K +NS RYRS+EPID 
Sbjct: 303 CPKSNQRLIHVALIPNYALKSLMQQWCLENNVNLLEPTKPCSFSK-TNSSRYRSDEPIDH 362

Query: 361 ISASKAASDAVKMTAEFLVGKLATGSPDVQRQAAYELRLLAKTGMDSRRMIAEAGAIPFL 420
           ISASKAASDAVKMTAEFLVGKLATGSPDVQRQAAYELRLLAKTGMDSRRMIAEAGAIPFL
Sbjct: 363 ISASKAASDAVKMTAEFLVGKLATGSPDVQRQAAYELRLLAKTGMDSRRMIAEAGAIPFL 422

Query: 421 VTLLRSGDPRIEENAVTALFNLAIFNNNKILIVAAGALDNITHILESGKTMEARENAAAT 480
           VTLLRSGDPRIEENAVTALFNLAIFNNNKILIVAAGA+DNITHILESGKTMEARENAAAT
Sbjct: 423 VTLLRSGDPRIEENAVTALFNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAAT 482

Query: 481 IFSLTMVDEFKISIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYKANKACIVGAG 540
           IFSLTMVDEFKISIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYKANKACIVGAG
Sbjct: 483 IFSLTMVDEFKISIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYKANKACIVGAG 542

Query: 541 AVPLLIELLTDEKAGITDDALQALSLVLGCSEGLQEIRKSRVLVPLLVDLLRFGSPNGKE 600
           AVPLLIELLTDEKAGITDDALQALSLVLGC EGLQEIRKSRVLVPLLVDLLRFGSPNGKE
Sbjct: 543 AVPLLIELLTDEKAGITDDALQALSLVLGCCEGLQEIRKSRVLVPLLVDLLRFGSPNGKE 602

Query: 601 SSITVLLGMCKDGGEEVGRRLLMNPRSIPSLQSLAADGSLKARRKADALLRLLNRCCFQS 660
           SSITVLLGMCKDGGEEVGRRLLMNPRS+PSLQSLAADGSLKARRKADALLRLLNRCCFQS
Sbjct: 603 SSITVLLGMCKDGGEEVGRRLLMNPRSVPSLQSLAADGSLKARRKADALLRLLNRCCFQS 662

Query: 661 QPC 664
           QPC
Sbjct: 663 QPC 664

BLAST of Carg05967 vs. NCBI nr
Match: XP_023521656.1 (U-box domain-containing protein 1-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1117.8 bits (2890), Expect = 0.0e+00
Identity = 579/598 (96.82%), Postives = 588/598 (98.33%), Query Frame = 0

Query: 66  SNSPQLPPSSLVCFTELFSVVRRVKLLIQSCEEGSCLWSLLQTELISNQFYQLVKEIGRV 125
           SNSPQLPPSSLVCFTELFSV+RRVKLLIQ+CEEGSCLWSLLQTELIS+QFYQLVKEIGRV
Sbjct: 2   SNSPQLPPSSLVCFTELFSVIRRVKLLIQTCEEGSCLWSLLQTELISHQFYQLVKEIGRV 61

Query: 126 LDILPLSFLKLTDDTREQVELLHNQAKRFEFSADEREVRRRDELLELMMNKKNKDLAEFF 185
           LDILPLSFLKLTDDTREQVELLHNQAKRFEFSADEREVRRRDELLELMMNKKNKDLAEFF
Sbjct: 62  LDILPLSFLKLTDDTREQVELLHNQAKRFEFSADEREVRRRDELLELMMNKKNKDLAEFF 121

Query: 186 SSVGLRSLMDCDEEISKLEVEALKQAGTGGIMVVSNINNLISLVRHAKTVLFSKTEQFNL 245
           SSVGLRSLMDCDEEISKLEVE  KQAGTGGIMVVSNINNLISLVRHAKTV+FSK EQFNL
Sbjct: 122 SSVGLRSLMDCDEEISKLEVEGRKQAGTGGIMVVSNINNLISLVRHAKTVVFSKKEQFNL 181

Query: 246 KFQHHSCSSISMDQIPDDFRCPISLDVMRDPVIVSSGHTYDRNSIAQWIDSGHHVCPKSN 305
           KFQHHSCSSISMDQIPDDFRCPISLD+MRDPVIVSSGHTYDRNSIAQWIDSGHHVCPKSN
Sbjct: 182 KFQHHSCSSISMDQIPDDFRCPISLDLMRDPVIVSSGHTYDRNSIAQWIDSGHHVCPKSN 241

Query: 306 QRLIHVALIPNYALKSLMQQWCQENNVNLFEPTKPCSFLKRSNSRRYRSEEPIDQISASK 365
           QRLIHVALIPNYALKSLMQQWC ENNVNL EPTKPCSF K +NS RYRS+EPID ISASK
Sbjct: 242 QRLIHVALIPNYALKSLMQQWCLENNVNLLEPTKPCSFSK-TNSSRYRSDEPIDHISASK 301

Query: 366 AASDAVKMTAEFLVGKLATGSPDVQRQAAYELRLLAKTGMDSRRMIAEAGAIPFLVTLLR 425
           AASDAVKMTAEFLVGKLATGSPDVQRQAAYELRLLAKTGMDSRRMIAEAGAIPFLVTLLR
Sbjct: 302 AASDAVKMTAEFLVGKLATGSPDVQRQAAYELRLLAKTGMDSRRMIAEAGAIPFLVTLLR 361

Query: 426 SGDPRIEENAVTALFNLAIFNNNKILIVAAGALDNITHILESGKTMEARENAAATIFSLT 485
           SGDPRIEENAVTALFNLAIFNNNKILIVAAGA+DNITHILESGKTMEARENAAATIFSLT
Sbjct: 362 SGDPRIEENAVTALFNLAIFNNNKILIVAAGAIDNITHILESGKTMEARENAAATIFSLT 421

Query: 486 MVDEFKISIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYKANKACIVGAGAVPLL 545
           MVDEFKISIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYKANKACIVGAGAVPLL
Sbjct: 422 MVDEFKISIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYKANKACIVGAGAVPLL 481

Query: 546 IELLTDEKAGITDDALQALSLVLGCSEGLQEIRKSRVLVPLLVDLLRFGSPNGKESSITV 605
           IELLTDEKAGITDDALQALSLVLGC EGLQEIRKSRVLVPLLVDLLRFGSPNGKESSITV
Sbjct: 482 IELLTDEKAGITDDALQALSLVLGCCEGLQEIRKSRVLVPLLVDLLRFGSPNGKESSITV 541

Query: 606 LLGMCKDGGEEVGRRLLMNPRSIPSLQSLAADGSLKARRKADALLRLLNRCCFQSQPC 664
           LLGMCKDGGEEVGRRLLMNPRS+PSLQSLAADGSLKARRKADALLRLLNRCCFQSQPC
Sbjct: 542 LLGMCKDGGEEVGRRLLMNPRSVPSLQSLAADGSLKARRKADALLRLLNRCCFQSQPC 598

BLAST of Carg05967 vs. NCBI nr
Match: XP_008441478.1 (PREDICTED: U-box domain-containing protein 13-like [Cucumis melo])

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 576/687 (83.84%), Postives = 624/687 (90.83%), Query Frame = 0

Query: 1   MDVALSSPIMAASAFLPHGSVLQSLIVLANEVCLEEKASFVQARTVSTMRRRIKLLAFLF 60
           MDVALSS + A+S+  P GS+L+SLI+L+NEV  EEKA F+ A+T+STMRRRIKLLAFLF
Sbjct: 3   MDVALSSMMAASSSLFPSGSLLESLILLSNEVAFEEKAPFIHAKTISTMRRRIKLLAFLF 62

Query: 61  EEVHESNSPQLPPSSLVCFTELFSVVRRVKLLIQSCEEGSCLWSLLQTELISNQFYQLVK 120
           EEV ESNSP LPPSS++C TE+FSV+RRVK+L QSCEEGSCLWSLLQTE ISNQFYQ VK
Sbjct: 63  EEVQESNSP-LPPSSILCLTEIFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFVK 122

Query: 121 EIGRVLDILPLSFLKLTDDTREQVELLHNQAKRFEFSADEREVRRRDELLELMMNK---- 180
           EIGRVLDILPLS LKLTDDTREQVELLH+QAKRFEFS + REV+RRDELL+LM NK    
Sbjct: 123 EIGRVLDILPLSLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDELLQLMSNKERNY 182

Query: 181 KNKDLA------EFFSSVGLRSLMDCDEEISKLEVEALKQAGTGGIMVVSNINNLISLVR 240
           KNK L       E FSSVGLR++MDCDEEISKLE EALKQAGTGGI+VVSNINNLISLV+
Sbjct: 183 KNKGLGEVGKIKEIFSSVGLRNMMDCDEEISKLEAEALKQAGTGGIIVVSNINNLISLVK 242

Query: 241 HAKTVLFSKTE------QFNLKFQH------HSCSSISMDQIPDDFRCPISLDVMRDPVI 300
           HAKTV+FSK E      ++NLKFQH      HS SS S+ QIPDDFRCPISLD MRDPVI
Sbjct: 243 HAKTVIFSKKENENDGGKYNLKFQHSNKHLDHSSSSNSLVQIPDDFRCPISLDFMRDPVI 302

Query: 301 VSSGHTYDRNSIAQWIDSGHHVCPKSNQRLIHVALIPNYALKSLMQQWCQENNVNLFEPT 360
           +SSGHTYDR SIAQWIDSGHHVCPKSNQRLIH+ALIPNYALKSLMQQWCQENN+N+ EPT
Sbjct: 303 ISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALKSLMQQWCQENNINMNEPT 362

Query: 361 KPCSF--LKRSNSRRYRSEEPIDQISASKAASDAVKMTAEFLVGKLATGSPDVQRQAAYE 420
           KP S   L+RSNS++Y SEEP+D ISASKAASDAVKMTAEFLVGKLATGSPD+QRQAAYE
Sbjct: 363 KPYSSFGLERSNSKKYLSEEPVDHISASKAASDAVKMTAEFLVGKLATGSPDIQRQAAYE 422

Query: 421 LRLLAKTGMDSRRMIAEAGAIPFLVTLLRSGDPRIEENAVTALFNLAIFNNNKILIVAAG 480
           LRLLAKTGMD+RRMIAEAGAIPFLVTLL+SGDPRIEENAVTALFNLAIFNNNKILIVAAG
Sbjct: 423 LRLLAKTGMDNRRMIAEAGAIPFLVTLLKSGDPRIEENAVTALFNLAIFNNNKILIVAAG 482

Query: 481 ALDNITHILESGKTMEARENAAATIFSLTMVDEFKISIGASPKAIPALVRLLKEGNSAGK 540
           A+DNITHILESGKTMEARENAAATI+SLTMVDEFKI+IGASPKAIPALVRLLKEGNSAGK
Sbjct: 483 AIDNITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPKAIPALVRLLKEGNSAGK 542

Query: 541 RDAATALCNLALYKANKACIVGAGAVPLLIELLTDEKAGITDDALQALSLVLGCSEGLQE 600
           RDAATALCNLALY ANKACIV AGAVPLLIELLTD+KAGITDDALQALSLVLGCSEGLQE
Sbjct: 543 RDAATALCNLALYNANKACIVVAGAVPLLIELLTDDKAGITDDALQALSLVLGCSEGLQE 602

Query: 601 IRKSRVLVPLLVDLLRFGSPNGKESSITVLLGMCKDGGEEVGRRLLMNPRSIPSLQSLAA 660
           IRKSRVLV LL+DLLRFGSP GK+SS+T+LLG+CKDGGEEV RRLL+NPRSIPSLQSLAA
Sbjct: 603 IRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEVARRLLINPRSIPSLQSLAA 662

Query: 661 DGSLKARRKADALLRLLNRCCFQSQPC 664
           DGSLKARRKADALLRLLNRCCFQSQPC
Sbjct: 663 DGSLKARRKADALLRLLNRCCFQSQPC 688

BLAST of Carg05967 vs. TAIR10
Match: AT5G01830.1 (ARM repeat superfamily protein)

HSP 1 Score: 310.8 bits (795), Expect = 2.0e-84
Identity = 216/642 (33.64%), Postives = 338/642 (52.65%), Query Frame = 0

Query: 23  QSLIVLANEVCLEEKASFVQARTVSTMRRRIKLLAFLFEE--VHESNSPQLPPSSLVCFT 82
           +SL + ++E+   +   F+  R   ++ R++K+LA +F+E  +  S       S+ +CF 
Sbjct: 35  RSLFLASHEISSMQPLPFILRRNSLSLIRKVKILASVFDELLLPRSQLVVYSQSAHLCFE 94

Query: 83  ELFSVVRRVKLLIQSCEEGSCLWSLLQTELISNQFYQLVKEIGRVLDILPLSFLKLTDDT 142
           E+  V++R+K LI  C   S LW LLQ ++++  F++LV ++  VLDILPL    L+DD 
Sbjct: 95  EMQIVMQRIKSLIDDCSRVSKLWLLLQIDIVAFNFHELVTDLSTVLDILPLHDFDLSDDA 154

Query: 143 REQVELLHNQAKRFEFSADEREVRRRDELLELMMNKKNK------DLAEFFSSVGLRSLM 202
           ++ + LL  Q        D R+V  R ++ + +   K++       L + F+ +GL    
Sbjct: 155 QDLISLLTKQCSDSVQFVDARDVALRRKVTDTIAGIKHQISPDHSTLIKIFNDLGLSDSA 214

Query: 203 DCDEEISKLEVEALKQAGTGGIMVVSNINNLISLVRHAKTVLFSKTEQFNLKFQHHSCSS 262
              +EI +LE E   Q         S   +LI LVR++K VL+  +      F+ H   S
Sbjct: 215 SLTDEIQRLEDEIQDQIDDRS---KSAAASLIGLVRYSKCVLYGPSTPAP-DFRRH--QS 274

Query: 263 ISMDQIPDDFRCPISLDVMRDPVIVSSGHTYDRNSIAQWIDSGHHVCPKSNQRLIHVALI 322
           +S   IP DFRCPI+L++MRDPV+V++G TYDR SI  WI SGH+ CPK+ Q L H +L+
Sbjct: 275 LSDANIPADFRCPITLELMRDPVVVATGQTYDRESIDLWIQSGHNTCPKTGQVLKHTSLV 334

Query: 323 PNYALKSLMQQWCQENNVNLFEPTKPCSFLKRSNSRRYRSEEPIDQISASKAASDAVKMT 382
           PN ALK+L+  WC++  +       P               EP    +  K A +  KM 
Sbjct: 335 PNRALKNLIVLWCRDQKI-------PFELYGDGGG------EP----APCKEAVEFTKMM 394

Query: 383 AEFLVGKLATGSPDVQRQAAYELRLLAKTGMDSRRMIAEAGAIPFLVTLLRSGDPRIEEN 442
             FL+ KL+           +ELR LAK+   +R  IAEAGAIP LV  L +  P ++ N
Sbjct: 395 VSFLIEKLSVAD---SNGVVFELRALAKSDTVARACIAEAGAIPKLVRYLATECPSLQIN 454

Query: 443 AVTALFNLAIFNNNKILIVAA-GALDNITHILESGKTMEARENAAATIFSLTMVDEFKIS 502
           AVT + NL+I   NK  I+   GAL+ +  +L SG T EA+ NAAAT+FSL  V  ++  
Sbjct: 455 AVTTILNLSILEQNKTRIMETDGALNGVIEVLRSGATWEAKANAAATLFSLAGVSAYRRR 514

Query: 503 IGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYKANKACIVGAGAVPLLIELLTDEK 562
           +G   + +  LV L K+G ++ KRDA  A+ NL   + N    V AG    ++    D  
Sbjct: 515 LGRKARVVSGLVDLAKQGPTSSKRDALVAILNLVAERENVGRFVEAG----VMGAAGDAF 574

Query: 563 AGITDDALQALSLVLGCSEGLQEIRKSRVLVPLLVDLLRFGSPNGKESSITVLLGMCKDG 622
             + ++A+  +  V+    GL  +  +  L+ LL +++R G+   +ES+   L+ MC+ G
Sbjct: 575 QELPEEAVAVVEAVVR-RGGLMAVSAAFSLIRLLGEVMREGADTTRESAAATLVTMCRKG 634

Query: 623 GEEVGRRLLMNPRSIPSLQSLAADGSLKARRKADALLRLLNR 656
           G E+   +   P     +  +   G+ +  RKA +L+R L R
Sbjct: 635 GSELVAEMAAIPGIERVIWEMIGAGTARGGRKAASLMRYLRR 645

BLAST of Carg05967 vs. TAIR10
Match: AT1G29340.1 (plant U-box 17)

HSP 1 Score: 309.7 bits (792), Expect = 4.4e-84
Identity = 224/676 (33.14%), Postives = 343/676 (50.74%), Query Frame = 0

Query: 20  SVLQSLIVLANEV--CLEEKASFVQARTVSTMRRRIKLLAFLFEEVHESN---------- 79
           +++Q+L  +++EV  C        Q +   ++ R+I++   LFE + +SN          
Sbjct: 31  ALVQTLASISSEVVSCFTSVRFSFQRKNARSLIRKIEIFVVLFEFLVDSNWGSTTXXXXX 90

Query: 80  -----SPQLPPSSLVCFTELFSVVRRVKLLIQSCEEGSCLWSLLQTELISNQFYQLVKEI 139
                      ++L+C  EL+ ++ R K+L+  C + S LW LLQ   IS  F+ L +EI
Sbjct: 91  XXXXXXXXXXXTALLCLKELYLLLYRSKILVDYCAQSSKLWLLLQNPSISGYFHDLNQEI 150

Query: 140 GRVLDILPLSFLKLTDDTREQVELLHNQAKRFEFSADEREVRRRDELLELMMNKKN---- 199
             +LD+LP++ L L+DD REQ+ELL  Q+++     D+ +   R+     +   +N    
Sbjct: 151 STLLDVLPVNDLGLSDDIREQIELLQRQSRKARLYIDKNDESLRESFYSFLDGFENGKIP 210

Query: 200 --KDLAEFF-SSVGLRSLMDCDEEISKLEVEALKQAG----TGGIMVVSNINNLISLVRH 259
              DL  FF   +G+R    C  EI  LE + +   G    TG +     IN  +++ R+
Sbjct: 211 SSVDLRMFFVEKLGIRDSKSCRSEIEFLEEQIVNHDGDLEPTGSV-----INGFVAITRY 270

Query: 260 AKTVLFSKTE-------QFNLKFQHHSCSSISMDQ----IPDDFRCPISLDVMRDPVIVS 319
            + +LF   E       + N K       +  +      +P DF CPISLD+M DPVI+S
Sbjct: 271 CRFLLFGFEEDGMEWWIENNPKKPRKGFVAQEIGDTFITVPKDFVCPISLDLMTDPVIIS 330

Query: 320 SGHTYDRNSIAQWIDSGHHVCPKSNQRLIHVALIPNYALKSLMQQWCQENNVNLFEPTKP 379
           +G TYDRNSIA+WI+ GH  CPK+ Q L+   ++PN ALK+L+ QWC  + ++ +E    
Sbjct: 331 TGQTYDRNSIARWIEEGHCTCPKTGQMLMDSRIVPNRALKNLIVQWCTASGIS-YE---- 390

Query: 380 CSFLKRSNSRRYRSEEPIDQISASKAASDAVKMTAEFLVGKLATGSPDVQRQAAYELRLL 439
            S    S +  + S  P      +KAA +A K T   L+  LA GS   Q  AA E+RLL
Sbjct: 391 -SEFTDSPNESFASALP------TKAAVEANKATVSILIKYLADGSQAAQTVAAREIRLL 450

Query: 440 AKTGMDSRRMIAEAGAIPFLVTLLRSGDPRIEENAVTALFNLAIFNNNKILIVAAG-ALD 499
           AKTG ++R  IAEAGAIP L  LL S +   +EN+VTA+ NL+I+  NK  I+  G  L+
Sbjct: 451 AKTGKENRAYIAEAGAIPHLCRLLTSENAIAQENSVTAMLNLSIYEKNKSRIMEEGDCLE 510

Query: 500 NITHILESGKTMEARENAAATIFSLTMVDEFKISIGASPKAIPALVRLLKEGNSAGKRDA 559
           +I  +L SG                                              GK+DA
Sbjct: 511 SIVSVLVSGLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPRGKKDA 570

Query: 560 ATALCNLALYKANKACIVGAGAVPLLIELLTDEKAGITDDALQALSLVLGCSEGLQEIRK 619
            TAL NL+ +  N + ++  G V  L+  L +E  G+ ++A  AL+L++  S G + I K
Sbjct: 571 VTALYNLSTHPDNCSRMIEGGGVSSLVGALKNE--GVAEEAAGALALLVRQSLGAEAIGK 630

Query: 620 SRVLVPLLVDLLRFGSPNGKESSITVLLGMCKDGGEEVGRRLLMNPRSIPSLQSLAADGS 656
               V  L+ ++R G+P GKE+++  LL +C+ GG  V  ++L  P     LQ+L   G+
Sbjct: 631 EDSAVAGLMGMMRCGTPRGKENAVAALLELCRSGGAAVAEKVLRAPAIAGLLQTLLFTGT 687

BLAST of Carg05967 vs. TAIR10
Match: AT1G60190.1 (ARM repeat superfamily protein)

HSP 1 Score: 267.3 bits (682), Expect = 2.5e-71
Identity = 193/657 (29.38%), Postives = 342/657 (52.05%), Query Frame = 0

Query: 20  SVLQSLIVLANEV-CLEEKASFVQARTVSTMRRRIKLLAFLFEEVH---ESNSPQLPPSS 79
           +++ SL+ LA E+   + K      R+V    R ++ L   FEE+       S     S 
Sbjct: 28  TLVDSLLQLAGEILSFKPKHFSTNKRSVKETLRHVQTLVIFFEELRIQIRVGSIPAGRSV 87

Query: 80  LVCFTELFSVVRRVKLLIQSC-EEGSCLWSLLQTELISNQFYQLVKEIGRVLDILPLSFL 139
           ++  +EL  + +++K L+  C  +G+ L+ L+ +  +S  F  L + I   LD  P+  +
Sbjct: 88  ILSLSELHVIFQKLKFLLDDCTRDGAKLYMLMNSGQVSAHFRDLTRSISTSLDTFPVRSV 147

Query: 140 KLTDDTREQVELLHNQAKRFEFSADEREVRRRD------ELLELMMNKKNKDLAEFFSSV 199
            L  +  E + L+  Q ++ E   D  + R  D       L E  +N  + ++      +
Sbjct: 148 DLPGEVNELIYLVMRQTRKSEARPDRDDKRAIDSVYWFFNLFENRINPNSDEILRVLDHI 207

Query: 200 GLRSLMDCDEEISKLEVEALKQAGTGGIMVVSNINNLISLVRHAKTVLFSKTEQFNLKFQ 259
           G+R   DC +EI  L  E     G    + +  ++NL+  + + + V+    +  + +  
Sbjct: 208 GVRKWRDCVKEIDFLREEI--SVGKKSNIEIELLSNLMGFICYCRCVILRGIDVDDEEKD 267

Query: 260 HHSCSSISMDQI-PDDFRCPISLDVMRDPVIVSSGHTYDRNSIAQWIDSGHHVCPKSNQR 319
                 + +  +  DD RCPISL++M DPV++ SGHTYDR+SI +W  SG+  CPK+ + 
Sbjct: 268 KEEDDLMMVRSLNVDDLRCPISLEIMSDPVVLESGHTYDRSSITKWFASGNITCPKTGKT 327

Query: 320 LIHVALIPNYALKSLMQQWCQENNVNLFEPTKPCSFLKRSNSRRYRSEEPIDQISASKAA 379
           L+   L+ N+++K ++Q + ++N V + +                + ++ +D ++ S AA
Sbjct: 328 LVSTVLVDNFSVKQVIQSYSKQNGVVMGQ----------------KGKKKVD-VAESLAA 387

Query: 380 SDAVKMTAEFLVGKLATGSPDVQRQAAYELRLLAKTGMDSRRMIAEAGAIPFLVTLLRSG 439
            +A K+TAEFL G+L  G  +   +A  E+R+L KT    R  + EAG +  L+ +LRS 
Sbjct: 388 EEAGKLTAEFLAGELIKGDEEEMVKALVEIRILTKTSTFYRSCLVEAGVVESLMKILRSD 447

Query: 440 DPRIEENAVTALFNLA--IFNNNKILIVAAGALDNITHILESGKTMEARENAAATIFSLT 499
           DPRI+ENA+  + NL+  I    +I+    G L  I  +L  G   E+R+ AAA +F L+
Sbjct: 448 DPRIQENAMAGIMNLSKDIAGKTRIVGEDGGGLRLIVEVLNDGARRESRQYAAAALFYLS 507

Query: 500 MVDEFKISIGASPKAIPALVRLLKE---GNSAGKRDAATALCNLALYKA-NKACIVGAGA 559
            + ++   IG    AIP LVR++K    G+SA KR+A  A+ +L + +  N   I+ AG 
Sbjct: 508 SLGDYSRLIGEISDAIPGLVRIVKSCDYGDSA-KRNALIAIRSLLMNQPDNHWRILAAGI 567

Query: 560 VPLLIELLTDEKA--GITDDALQALSLVLGCSEGLQEIRKSRVLVPLLVDLLRFG--SPN 619
           VP+L++L+  E+   G+T D++  L+ +    +G+  + + R  + L V +L     SP 
Sbjct: 568 VPVLLDLVKSEEISDGVTADSMAILAKMAEYPDGMISVLR-RGGLKLAVKILGSSEVSPA 627

Query: 620 GKESSITVLLGMCKDGGEEVGRRLLMNPRSIPSLQSLAADGSLKARRKADALLRLLN 655
            K+  + +LL +C +GG +V   L  NP  + SL + +++G L   +KA AL+++++
Sbjct: 628 TKQHCVALLLNLCHNGGSDVVGSLAKNPSIMGSLYTASSNGELGGGKKASALIKMIH 663

BLAST of Carg05967 vs. TAIR10
Match: AT1G10560.1 (plant U-box 18)

HSP 1 Score: 231.9 bits (590), Expect = 1.2e-60
Identity = 183/676 (27.07%), Postives = 340/676 (50.30%), Query Frame = 0

Query: 5   LSSPIMAASAFLPHGSVLQSLIVLANEVCLEEKASF-VQARTVSTMRRRIKLLAFLFEE- 64
           L+ P +  S  +   ++L SLI LA ++   +   F    ++     RRI+ L  +FEE 
Sbjct: 13  LTFPTVEPSESISIVTLLDSLIQLAGDILTFKSKHFSTNKQSFRETLRRIQNLLVVFEEI 72

Query: 65  ---VHESNSPQLPPSSLVCFTELFSVVRRVKLLIQSC-EEGSCLWSLLQTELISNQFYQL 124
              +  S       ++     E+    +++K L++ C  +G+ L  ++ ++ +S+    L
Sbjct: 73  RIRIRNSRRYFHDSAAASSLKEIHVGFQKLKFLLEDCTRDGARLCMMMNSDQVSDHLRVL 132

Query: 125 VKEIGRVLDILPLSFLKLTDDTREQVELLHNQAKRFEF---SADEREVRRRDELLELMMN 184
            + I   L   P++ + LT +  E ++L+  QA+++     + D+R V   + +L L +N
Sbjct: 133 TRSISTSLSAFPVASVDLTTEVNELIDLVVRQARKYGVQPETNDKRAVSSINRILALFVN 192

Query: 185 KKNKD---LAEFFSSVGLRSLMDCDEEIS----KLEVEALKQAGTGGIMVVSNINNLISL 244
           +   D   +      VG+R   DC +EI+    +++ E L +        V  +++L+  
Sbjct: 193 RVVPDPDEINRILDHVGIRKWGDCVKEINFLGEEIDAERLDEKKKKSSDQVELLSSLMGF 252

Query: 245 VRHAKTVLFSKTEQFNLKFQHHSCSSISMDQI----PDDFRCPISLDVMRDPVIVSSGHT 304
           + + + ++  + E+ +    H        D I     +D  CPISL++M DPV++ +GHT
Sbjct: 253 ICYCRCIILGRIERDDHHNHHEDGIKKDHDLIRGLKVEDLLCPISLEIMTDPVVIETGHT 312

Query: 305 YDRNSIAQWIDSGHHVCPKSNQRLIHVALIPNYALKSLMQQWCQENNVNLFEPTKPCSFL 364
           YDR+SI +W  SG+  CP + + L    L+ N +++ ++++ C+ N + L          
Sbjct: 313 YDRSSITKWFGSGNITCPITGKILTSTELVDNVSVRQVIRKHCKTNGIVL---------- 372

Query: 365 KRSNSRRYRSEEPIDQISASKAASDAVKMTAEFLVGKLATGSPDVQRQAAYELRLLAKTG 424
               SRR +S +  D +  S AA  A K+ A+FL  +L  G  ++  +A  E+R+  KT 
Sbjct: 373 -AGISRRRKSHD--DVVPESLAAKGAGKLIAKFLTSELINGGEEMIYRAVREIRVQTKTS 432

Query: 425 MDSRRMIAEAGAIPFLVTLLRSGDPRIEENAVTALFNLAIFNNNKILIVAAGALDNITHI 484
             +R  + +AGA+  L+ LL S D RI+ENA+  + NL+     K  I   G L  +  I
Sbjct: 433 SFNRSCLVKAGAVTPLLKLLSSVDIRIQENAMAGILNLSKHVTGKSKIAGEG-LKILVEI 492

Query: 485 LESGKTMEARENAAATIFSLTMVDEFKISIGASPKAIPALVRLLK--EGNSAGKRDAATA 544
           L  G   E R  +A+ +F L+ V+++   IG +P AIP L+ ++K  +   + KR A  A
Sbjct: 493 LNEGAKTETRLYSASALFYLSSVEDYSRLIGENPDAIPGLMNIVKGDDYGDSAKRSALLA 552

Query: 545 LCNLALYKANKACIVGAGAVPLLIELLT--DEKAGITDDALQALSLVLGCSEG-LQEIRK 604
           +  L +   N   ++ AGAVP+L++LL   +   G+T D L  L+ +    +G +  IR+
Sbjct: 553 VMGLLMQSDNHWRVLAAGAVPILLDLLRSGEISGGLTADCLATLAKLAEYPDGTIGVIRR 612

Query: 605 SRVLVPLLVDLLRFGSPNG-KESSITVLLGMCKDGGEEVGRRLLMNPRSIPSLQSLAADG 655
             + + + +      SP   K+  + ++L +C +GG +V   L+ N   + SL ++ ++G
Sbjct: 613 GGLKLAVKILSSSEDSPVAVKQHCVGLILNLCLNGGRDVVGVLVKNSLVMGSLYTVLSNG 672

BLAST of Carg05967 vs. TAIR10
Match: AT3G46510.1 (plant U-box 13)

HSP 1 Score: 225.3 bits (573), Expect = 1.1e-58
Identity = 155/441 (35.15%), Postives = 234/441 (53.06%), Query Frame = 0

Query: 20  SVLQSLIVLANEVCLEEKASFVQARTVSTMRRRIKLLAFLFEEVHESNSPQLPPSSLVCF 79
           S  QSLI + NE+           +    + RR+KLL  +FEE+ ESN P +   +L   
Sbjct: 7   SAAQSLIDVVNEIAAISDYRITVKKLCYNLARRLKLLVPMFEEIRESNEP-ISEDTLKTL 66

Query: 80  TELFSVVRRVKLLIQSCEEGSCLWSLLQTELISNQFYQLVKEIGRVLDILPLSFLKLTDD 139
             L   +   K  ++ C +GS ++ +++ E ++++  ++  ++ + L  +P   L ++D+
Sbjct: 67  MNLKEAMCSAKDYLKFCSQGSKIYLVMEREQVTSKLMEVSVKLEQSLSQIPYEELDISDE 126

Query: 140 TREQVELLHNQAKRFEFSADEREVRRRDELLELMMNKKNKD-----LAEFFSSVGLRSLM 199
            REQVEL+ +Q +R +   D  +    ++L  L     + D     L      + L  + 
Sbjct: 127 VREQVELVLSQFRRAKGRVDVSDDELYEDLQSLCNKSSDVDAYQPVLERVAKKLHLMEIP 186

Query: 200 DCDEEISKLEVEALKQAGTGGIMVVSNINNLISLVRHAKTVLFSKTEQFNLKFQHHSCSS 259
           D  +E   L        G  G     NI  +  +++  K   F +TE  N + Q    +S
Sbjct: 187 DLAQESVALHEMVASSGGDVG----ENIEEMAMVLKMIKD--FVQTEDDNGEEQKVGVNS 246

Query: 260 ISMDQ-----------IPDDFRCPISLDVMRDPVIVSSGHTYDRNSIAQWIDSGHHVCPK 319
            S  Q           IPDDFRCPISL++MRDPVIVSSG TY+R  I +WI+ GH  CPK
Sbjct: 247 RSNGQTSTAASQKIPVIPDDFRCPISLEMMRDPVIVSSGQTYERTCIEKWIEGGHSTCPK 306

Query: 320 SNQRLIHVALIPNYALKSLMQQWCQENNVNLFEPTKPCSFLKRSNSRRYRSEEPIDQISA 379
           + Q L    L PNY L+SL+ QWC+ N++   EP KP S L+              ++S+
Sbjct: 307 TQQALTSTTLTPNYVLRSLIAQWCEANDI---EPPKPPSSLRPR------------KVSS 366

Query: 380 SKAASDAVKMTAEFLVGKLATGSPDVQRQAAYELRLLAKTGMDSRRMIAEAGAIPFLVTL 439
             + ++A K+  E L+ +LA G+P+ QR AA E+RLLAK   D+R  IAEAGAIP LV L
Sbjct: 367 FSSPAEANKI--EDLMWRLAYGNPEDQRSAAGEIRLLAKRNADNRVAIAEAGAIPLLVGL 423

Query: 440 LRSGDPRIEENAVTALFNLAI 445
           L + D RI+E++VTAL NL+I
Sbjct: 427 LSTPDSRIQEHSVTALLNLSI 423

BLAST of Carg05967 vs. Swiss-Prot
Match: sp|E4NKF8|PUB1_MEDTR (U-box domain-containing protein 1 OS=Medicago truncatula OX=3880 GN=PUB1 PE=1 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 1.4e-188
Identity = 380/689 (55.15%), Postives = 464/689 (67.34%), Query Frame = 0

Query: 7   SPIMAASAFLPHGSVLQSLIVLANEVCLEEKASFVQARTVSTMRRRIKLLAFLFEEVHES 66
           S +M +   LP  S+L SLI+++NEV   +K   VQ + VS+M RRIKLL+ LFEE+ ES
Sbjct: 6   SKMMISPGLLPTESLLDSLILISNEVSSMQKFPLVQIKNVSSMIRRIKLLSSLFEEIQES 65

Query: 67  NSPQLPPSSLVCFTELFSVVRRVKLLIQSCEEGSCLWSLLQTELISNQFYQLVKEIGRVL 126
           +SP LPPSS++CF E+FSV+ RVK+LIQ C +GS LWSL+Q + ISNQF+ LVKE+GR L
Sbjct: 66  DSP-LPPSSILCFIEIFSVITRVKVLIQECTDGSSLWSLIQLDFISNQFFVLVKEMGRAL 125

Query: 127 DILPLSFLKLTDDTREQVELLHNQAKRFEFS--ADEREVRRRDELLELM-----MNKKNK 186
           DILPL+ L +  D +EQV+LLH Q+KR E     D REV+RR+ L E+M      NKK  
Sbjct: 126 DILPLNLLNVAQDIKEQVDLLHKQSKRVELELFIDPREVQRRENLFEVMSKNCLQNKKTN 185

Query: 187 D---------LAEFFSSVGLRSLMDCDEEISKLEVEALKQAGTGGIMVVSNINNLISLVR 246
           +         + E   S+GLR+L D  EEISKLEVEA  QAGTGG++VVSNINNL+SLV 
Sbjct: 186 NNKGFIDFVKVEEIMCSIGLRTLSDYVEEISKLEVEAQNQAGTGGLIVVSNINNLMSLVS 245

Query: 247 HAKTVLF-------------------SKTEQFNLKFQHHSCSSISMDQIPDDFRCPISLD 306
           + K+++F                   SK              S+    IPD+FRCPISLD
Sbjct: 246 YTKSMVFRNDGESEECKPISMFLYNKSKXXXXXXXXXXXXXXSMMTVNIPDEFRCPISLD 305

Query: 307 VMRDPVIVSSGHTYDRNSIAQWIDSGHHVCPKSNQRLIHVALIPNYALKSLMQQWCQENN 366
           +MRDPVIVSSGHTYDR SIA+WI+SGHH CPKS QRLIH ALIPNYALKSL+ QWC ENN
Sbjct: 306 LMRDPVIVSSGHTYDRISIAEWINSGHHTCPKSGQRLIHTALIPNYALKSLVHQWCYENN 365

Query: 367 VNLFEP-TKPCSFLKRSNSRRYRSEEPIDQISASKAASDAVKMTAEFLVGKLATGSPDVQ 426
           V + E  TK       S+S+R+++E  ID IS +KA+ DAVKMTAEFLVGKLATGS D+Q
Sbjct: 366 VKMNEAITKN----NNSSSKRHKNENAIDHISENKASKDAVKMTAEFLVGKLATGSTDIQ 425

Query: 427 RQAAYELRLLAKTGMDSRRMIAEAGAIPFLVTLLRSGDPRIEENAVTALFNLAIFNNNKI 486
           RQ+AYE+RLLAKTGMD+RR+IAE GAIPFLVTLL S D RI+E+ VTALFNL+I++NNKI
Sbjct: 426 RQSAYEIRLLAKTGMDNRRIIAEVGAIPFLVTLLVSKDSRIQEHVVTALFNLSIYDNNKI 485

Query: 487 LIVAAGALDNITHILESGKTMEARENAAATIFSLTMVDEFKISIGASPKAIPALVRLLKE 546
           LI+AAGA+DNI  +LE                                            
Sbjct: 486 LIMAAGAIDNIVEVLEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 545

Query: 547 GNSAGKRDAATALCNLALYKANKACIVGAGAVPLLIELLTDEKAGITDDALQALSLVLGC 606
                                       +GAV LL+ELL D+KAGITDD+L  L+++LGC
Sbjct: 546 XXXXXXXXXXXXXXXXXXXXXXXXXXXXSGAVTLLVELLMDDKAGITDDSLAVLAVLLGC 605

Query: 607 SEGLQEIRKSRVLVPLLVDLLRFGSPNGKESSITVLLGMCKDGGEEVGRRLLMNPRSIPS 660
           SEGL+EI+ S+ LVPLL+DLLRFGS  GKE+SIT+LLG+CK+ GE V  RLL NPRSIPS
Sbjct: 606 SEGLEEIKNSKSLVPLLIDLLRFGSVKGKENSITLLLGLCKEEGELVAMRLLANPRSIPS 665

BLAST of Carg05967 vs. Swiss-Prot
Match: sp|Q9LZW3|PUB16_ARATH (U-box domain-containing protein 16 OS=Arabidopsis thaliana OX=3702 GN=PUB16 PE=2 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 3.6e-83
Identity = 216/642 (33.64%), Postives = 338/642 (52.65%), Query Frame = 0

Query: 23  QSLIVLANEVCLEEKASFVQARTVSTMRRRIKLLAFLFEE--VHESNSPQLPPSSLVCFT 82
           +SL + ++E+   +   F+  R   ++ R++K+LA +F+E  +  S       S+ +CF 
Sbjct: 35  RSLFLASHEISSMQPLPFILRRNSLSLIRKVKILASVFDELLLPRSQLVVYSQSAHLCFE 94

Query: 83  ELFSVVRRVKLLIQSCEEGSCLWSLLQTELISNQFYQLVKEIGRVLDILPLSFLKLTDDT 142
           E+  V++R+K LI  C   S LW LLQ ++++  F++LV ++  VLDILPL    L+DD 
Sbjct: 95  EMQIVMQRIKSLIDDCSRVSKLWLLLQIDIVAFNFHELVTDLSTVLDILPLHDFDLSDDA 154

Query: 143 REQVELLHNQAKRFEFSADEREVRRRDELLELMMNKKNK------DLAEFFSSVGLRSLM 202
           ++ + LL  Q        D R+V  R ++ + +   K++       L + F+ +GL    
Sbjct: 155 QDLISLLTKQCSDSVQFVDARDVALRRKVTDTIAGIKHQISPDHSTLIKIFNDLGLSDSA 214

Query: 203 DCDEEISKLEVEALKQAGTGGIMVVSNINNLISLVRHAKTVLFSKTEQFNLKFQHHSCSS 262
              +EI +LE E   Q         S   +LI LVR++K VL+  +      F+ H   S
Sbjct: 215 SLTDEIQRLEDEIQDQIDDRS---KSAAASLIGLVRYSKCVLYGPSTPAP-DFRRH--QS 274

Query: 263 ISMDQIPDDFRCPISLDVMRDPVIVSSGHTYDRNSIAQWIDSGHHVCPKSNQRLIHVALI 322
           +S   IP DFRCPI+L++MRDPV+V++G TYDR SI  WI SGH+ CPK+ Q L H +L+
Sbjct: 275 LSDANIPADFRCPITLELMRDPVVVATGQTYDRESIDLWIQSGHNTCPKTGQVLKHTSLV 334

Query: 323 PNYALKSLMQQWCQENNVNLFEPTKPCSFLKRSNSRRYRSEEPIDQISASKAASDAVKMT 382
           PN ALK+L+  WC++  +       P               EP    +  K A +  KM 
Sbjct: 335 PNRALKNLIVLWCRDQKI-------PFELYGDGGG------EP----APCKEAVEFTKMM 394

Query: 383 AEFLVGKLATGSPDVQRQAAYELRLLAKTGMDSRRMIAEAGAIPFLVTLLRSGDPRIEEN 442
             FL+ KL+           +ELR LAK+   +R  IAEAGAIP LV  L +  P ++ N
Sbjct: 395 VSFLIEKLSVAD---SNGVVFELRALAKSDTVARACIAEAGAIPKLVRYLATECPSLQIN 454

Query: 443 AVTALFNLAIFNNNKILIVAA-GALDNITHILESGKTMEARENAAATIFSLTMVDEFKIS 502
           AVT + NL+I   NK  I+   GAL+ +  +L SG T EA+ NAAAT+FSL  V  ++  
Sbjct: 455 AVTTILNLSILEQNKTRIMETDGALNGVIEVLRSGATWEAKANAAATLFSLAGVSAYRRR 514

Query: 503 IGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYKANKACIVGAGAVPLLIELLTDEK 562
           +G   + +  LV L K+G ++ KRDA  A+ NL   + N    V AG    ++    D  
Sbjct: 515 LGRKARVVSGLVDLAKQGPTSSKRDALVAILNLVAERENVGRFVEAG----VMGAAGDAF 574

Query: 563 AGITDDALQALSLVLGCSEGLQEIRKSRVLVPLLVDLLRFGSPNGKESSITVLLGMCKDG 622
             + ++A+  +  V+    GL  +  +  L+ LL +++R G+   +ES+   L+ MC+ G
Sbjct: 575 QELPEEAVAVVEAVVR-RGGLMAVSAAFSLIRLLGEVMREGADTTRESAAATLVTMCRKG 634

Query: 623 GEEVGRRLLMNPRSIPSLQSLAADGSLKARRKADALLRLLNR 656
           G E+   +   P     +  +   G+ +  RKA +L+R L R
Sbjct: 635 GSELVAEMAAIPGIERVIWEMIGAGTARGGRKAASLMRYLRR 645

BLAST of Carg05967 vs. Swiss-Prot
Match: sp|Q9C7R6|PUB17_ARATH (U-box domain-containing protein 17 OS=Arabidopsis thaliana OX=3702 GN=PUB17 PE=2 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 7.9e-83
Identity = 224/676 (33.14%), Postives = 343/676 (50.74%), Query Frame = 0

Query: 20  SVLQSLIVLANEV--CLEEKASFVQARTVSTMRRRIKLLAFLFEEVHESN---------- 79
           +++Q+L  +++EV  C        Q +   ++ R+I++   LFE + +SN          
Sbjct: 31  ALVQTLASISSEVVSCFTSVRFSFQRKNARSLIRKIEIFVVLFEFLVDSNWGSTTXXXXX 90

Query: 80  -----SPQLPPSSLVCFTELFSVVRRVKLLIQSCEEGSCLWSLLQTELISNQFYQLVKEI 139
                      ++L+C  EL+ ++ R K+L+  C + S LW LLQ   IS  F+ L +EI
Sbjct: 91  XXXXXXXXXXXTALLCLKELYLLLYRSKILVDYCAQSSKLWLLLQNPSISGYFHDLNQEI 150

Query: 140 GRVLDILPLSFLKLTDDTREQVELLHNQAKRFEFSADEREVRRRDELLELMMNKKN---- 199
             +LD+LP++ L L+DD REQ+ELL  Q+++     D+ +   R+     +   +N    
Sbjct: 151 STLLDVLPVNDLGLSDDIREQIELLQRQSRKARLYIDKNDESLRESFYSFLDGFENGKIP 210

Query: 200 --KDLAEFF-SSVGLRSLMDCDEEISKLEVEALKQAG----TGGIMVVSNINNLISLVRH 259
              DL  FF   +G+R    C  EI  LE + +   G    TG +     IN  +++ R+
Sbjct: 211 SSVDLRMFFVEKLGIRDSKSCRSEIEFLEEQIVNHDGDLEPTGSV-----INGFVAITRY 270

Query: 260 AKTVLFSKTE-------QFNLKFQHHSCSSISMDQ----IPDDFRCPISLDVMRDPVIVS 319
            + +LF   E       + N K       +  +      +P DF CPISLD+M DPVI+S
Sbjct: 271 CRFLLFGFEEDGMEWWIENNPKKPRKGFVAQEIGDTFITVPKDFVCPISLDLMTDPVIIS 330

Query: 320 SGHTYDRNSIAQWIDSGHHVCPKSNQRLIHVALIPNYALKSLMQQWCQENNVNLFEPTKP 379
           +G TYDRNSIA+WI+ GH  CPK+ Q L+   ++PN ALK+L+ QWC  + ++ +E    
Sbjct: 331 TGQTYDRNSIARWIEEGHCTCPKTGQMLMDSRIVPNRALKNLIVQWCTASGIS-YE---- 390

Query: 380 CSFLKRSNSRRYRSEEPIDQISASKAASDAVKMTAEFLVGKLATGSPDVQRQAAYELRLL 439
            S    S +  + S  P      +KAA +A K T   L+  LA GS   Q  AA E+RLL
Sbjct: 391 -SEFTDSPNESFASALP------TKAAVEANKATVSILIKYLADGSQAAQTVAAREIRLL 450

Query: 440 AKTGMDSRRMIAEAGAIPFLVTLLRSGDPRIEENAVTALFNLAIFNNNKILIVAAG-ALD 499
           AKTG ++R  IAEAGAIP L  LL S +   +EN+VTA+ NL+I+  NK  I+  G  L+
Sbjct: 451 AKTGKENRAYIAEAGAIPHLCRLLTSENAIAQENSVTAMLNLSIYEKNKSRIMEEGDCLE 510

Query: 500 NITHILESGKTMEARENAAATIFSLTMVDEFKISIGASPKAIPALVRLLKEGNSAGKRDA 559
           +I  +L SG                                              GK+DA
Sbjct: 511 SIVSVLVSGLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPRGKKDA 570

Query: 560 ATALCNLALYKANKACIVGAGAVPLLIELLTDEKAGITDDALQALSLVLGCSEGLQEIRK 619
            TAL NL+ +  N + ++  G V  L+  L +E  G+ ++A  AL+L++  S G + I K
Sbjct: 571 VTALYNLSTHPDNCSRMIEGGGVSSLVGALKNE--GVAEEAAGALALLVRQSLGAEAIGK 630

Query: 620 SRVLVPLLVDLLRFGSPNGKESSITVLLGMCKDGGEEVGRRLLMNPRSIPSLQSLAADGS 656
               V  L+ ++R G+P GKE+++  LL +C+ GG  V  ++L  P     LQ+L   G+
Sbjct: 631 EDSAVAGLMGMMRCGTPRGKENAVAALLELCRSGGAAVAEKVLRAPAIAGLLQTLLFTGT 687

BLAST of Carg05967 vs. Swiss-Prot
Match: sp|O80742|PUB19_ARATH (U-box domain-containing protein 19 OS=Arabidopsis thaliana OX=3702 GN=PUB19 PE=2 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 4.5e-70
Identity = 193/657 (29.38%), Postives = 342/657 (52.05%), Query Frame = 0

Query: 20  SVLQSLIVLANEV-CLEEKASFVQARTVSTMRRRIKLLAFLFEEVH---ESNSPQLPPSS 79
           +++ SL+ LA E+   + K      R+V    R ++ L   FEE+       S     S 
Sbjct: 28  TLVDSLLQLAGEILSFKPKHFSTNKRSVKETLRHVQTLVIFFEELRIQIRVGSIPAGRSV 87

Query: 80  LVCFTELFSVVRRVKLLIQSC-EEGSCLWSLLQTELISNQFYQLVKEIGRVLDILPLSFL 139
           ++  +EL  + +++K L+  C  +G+ L+ L+ +  +S  F  L + I   LD  P+  +
Sbjct: 88  ILSLSELHVIFQKLKFLLDDCTRDGAKLYMLMNSGQVSAHFRDLTRSISTSLDTFPVRSV 147

Query: 140 KLTDDTREQVELLHNQAKRFEFSADEREVRRRD------ELLELMMNKKNKDLAEFFSSV 199
            L  +  E + L+  Q ++ E   D  + R  D       L E  +N  + ++      +
Sbjct: 148 DLPGEVNELIYLVMRQTRKSEARPDRDDKRAIDSVYWFFNLFENRINPNSDEILRVLDHI 207

Query: 200 GLRSLMDCDEEISKLEVEALKQAGTGGIMVVSNINNLISLVRHAKTVLFSKTEQFNLKFQ 259
           G+R   DC +EI  L  E     G    + +  ++NL+  + + + V+    +  + +  
Sbjct: 208 GVRKWRDCVKEIDFLREEI--SVGKKSNIEIELLSNLMGFICYCRCVILRGIDVDDEEKD 267

Query: 260 HHSCSSISMDQI-PDDFRCPISLDVMRDPVIVSSGHTYDRNSIAQWIDSGHHVCPKSNQR 319
                 + +  +  DD RCPISL++M DPV++ SGHTYDR+SI +W  SG+  CPK+ + 
Sbjct: 268 KEEDDLMMVRSLNVDDLRCPISLEIMSDPVVLESGHTYDRSSITKWFASGNITCPKTGKT 327

Query: 320 LIHVALIPNYALKSLMQQWCQENNVNLFEPTKPCSFLKRSNSRRYRSEEPIDQISASKAA 379
           L+   L+ N+++K ++Q + ++N V + +                + ++ +D ++ S AA
Sbjct: 328 LVSTVLVDNFSVKQVIQSYSKQNGVVMGQ----------------KGKKKVD-VAESLAA 387

Query: 380 SDAVKMTAEFLVGKLATGSPDVQRQAAYELRLLAKTGMDSRRMIAEAGAIPFLVTLLRSG 439
            +A K+TAEFL G+L  G  +   +A  E+R+L KT    R  + EAG +  L+ +LRS 
Sbjct: 388 EEAGKLTAEFLAGELIKGDEEEMVKALVEIRILTKTSTFYRSCLVEAGVVESLMKILRSD 447

Query: 440 DPRIEENAVTALFNLA--IFNNNKILIVAAGALDNITHILESGKTMEARENAAATIFSLT 499
           DPRI+ENA+  + NL+  I    +I+    G L  I  +L  G   E+R+ AAA +F L+
Sbjct: 448 DPRIQENAMAGIMNLSKDIAGKTRIVGEDGGGLRLIVEVLNDGARRESRQYAAAALFYLS 507

Query: 500 MVDEFKISIGASPKAIPALVRLLKE---GNSAGKRDAATALCNLALYKA-NKACIVGAGA 559
            + ++   IG    AIP LVR++K    G+SA KR+A  A+ +L + +  N   I+ AG 
Sbjct: 508 SLGDYSRLIGEISDAIPGLVRIVKSCDYGDSA-KRNALIAIRSLLMNQPDNHWRILAAGI 567

Query: 560 VPLLIELLTDEKA--GITDDALQALSLVLGCSEGLQEIRKSRVLVPLLVDLLRFG--SPN 619
           VP+L++L+  E+   G+T D++  L+ +    +G+  + + R  + L V +L     SP 
Sbjct: 568 VPVLLDLVKSEEISDGVTADSMAILAKMAEYPDGMISVLR-RGGLKLAVKILGSSEVSPA 627

Query: 620 GKESSITVLLGMCKDGGEEVGRRLLMNPRSIPSLQSLAADGSLKARRKADALLRLLN 655
            K+  + +LL +C +GG +V   L  NP  + SL + +++G L   +KA AL+++++
Sbjct: 628 TKQHCVALLLNLCHNGGSDVVGSLAKNPSIMGSLYTASSNGELGGGKKASALIKMIH 663

BLAST of Carg05967 vs. Swiss-Prot
Match: sp|Q6EUK7|PUB04_ORYSJ (U-box domain-containing protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB4 PE=2 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.5e-65
Identity = 191/635 (30.08%), Postives = 309/635 (48.66%), Query Frame = 0

Query: 42  QARTVSTMRRRIKLLAFLFEEV--HESNSPQLPPSSLVCFTELFSVVRRVKLLIQSCEEG 101
           Q R V  + RR+ LL+ + E +    + +     ++ +CF EL+ V+ R +LL+      
Sbjct: 62  QRRNVDALARRLALLSAILESILLDTAAAGAFSDAANLCFRELYVVLFRAELLVSYVASA 121

Query: 102 SCLWSLLQTELISNQFYQLVKEIGRVLDILPLSFLKLTDDTREQVELL--HNQAKRFEFS 161
              W+LL++  ++  F  L  E+  VLD+LP + L+L+ D    ++LL  H + +     
Sbjct: 122 GRAWALLRSPHLAASFRDLDAELAVVLDVLPAASLRLSHDATGLLDLLRAHCRCRAPAQY 181

Query: 162 ADEREVRRRDELLELMMN------KKNKDLAEFFSSVGLRSLMDCDEEISKLEVEALKQA 221
            D  E   R+ L++ +          +  L    + +G+ +   C  EI  LE + L Q 
Sbjct: 182 HDPDEAALRERLMDALRQFDLGQPPDHPSLQSLLADMGISTAASCRAEIDYLEEQILSQE 241

Query: 222 GTGGIMVVSNINNLISLVRHAKTVLFSKT-----EQFNLKFQHHSCSSI-----SMDQIP 281
               + +V ++   ++L+R+    +F  +       + L        SI     +   +P
Sbjct: 242 EDTDLPLVGSV---LALLRYCLFAVFDPSNAKALRDWPLSGNRQRLLSIGGGDDTSFSVP 301

Query: 282 DDFRCPISLDVMRDPVIVSSGHTYDRNSIAQWIDSGHHVCPKSNQRLIHVALIPNYALKS 341
            +F CPISLD+MRDPV+ S+G TYDR SI QWI+ GH  CP S Q L    L+PN AL+S
Sbjct: 302 KEFSCPISLDLMRDPVVASTGQTYDRPSIIQWIEEGHSTCPNSGQTLADHRLVPNRALRS 361

Query: 342 LMQQWCQENNVNLFEPTKPCSFLKRSNSRRYRSEEPIDQISASKAASDAVKMTAEFLVGK 401
           L+ QWC    +    P         SN       E +    +S+AA +A K TA  LV  
Sbjct: 362 LISQWCGVYGLQYDSP--------ESNE---GMAECVAASCSSRAAMEANKATARILVRM 421

Query: 402 LATGSPDVQRQAAYELRLLAKTGMDSRRMIAEAGAIPFLVTLLRSGDPRIEENAVTALFN 461
           L  GS +V+  AA E+RLLAKTG  +R  IA+ GAIP L  LL S D   +ENAVTAL N
Sbjct: 422 LEDGSENVKAVAAKEIRLLAKTGKQNRAFIADLGAIPLLCRLLLSNDWMAQENAVTALLN 481

Query: 462 LAIFNNNKILIV-AAGALDNITHILESGKTMEARENAAATIFSLTMVDEFKISIGASPKA 521
           L+IF  NK  I+   G L  I  +L++G T E                            
Sbjct: 482 LSIFEPNKGRIMEQEGCLRLIVGVLQNGWTTEXXXXXXXXXXXXXXXXXXXXXXXXXXXX 541

Query: 522 IPALVRLLKEGNSAGKRDAATALCNLALYKANKACIVGAGAVPLLIELLTDEKAGITDDA 581
                             A  AL NL+ +  + A ++ + AV  LI+ L ++   ++++A
Sbjct: 542 XXXXXXXXXXXXXXXXXXAVMALFNLSTHPESSARMLESCAVVALIQSLRNDT--VSEEA 601

Query: 582 LQALSLVLGCSEGLQEIRKSRVLVPLLVDLLRFGSPNGKESSITVLLGMCKDGGEEVGRR 641
             AL+L++     +  +  S  ++  LV L+R G+P GKE++++ L  +C+ GG  + +R
Sbjct: 602 AGALALLMKQPSIVHLVGSSETVITSLVGLMRRGTPKGKENAVSALYEICRRGGSALVQR 661

Query: 642 LLMNPRSIPSLQSLAADGSLKARRKADALLRLLNR 656
           +   P     +Q++  +G+ +A++KA  ++++  R
Sbjct: 662 VAKIPGLNTVIQTITLNGTKRAKKKASLIVKMCQR 680

BLAST of Carg05967 vs. TrEMBL
Match: tr|A0A1S3B3K6|A0A1S3B3K6_CUCME (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485586 PE=4 SV=1)

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 576/687 (83.84%), Postives = 624/687 (90.83%), Query Frame = 0

Query: 1   MDVALSSPIMAASAFLPHGSVLQSLIVLANEVCLEEKASFVQARTVSTMRRRIKLLAFLF 60
           MDVALSS + A+S+  P GS+L+SLI+L+NEV  EEKA F+ A+T+STMRRRIKLLAFLF
Sbjct: 3   MDVALSSMMAASSSLFPSGSLLESLILLSNEVAFEEKAPFIHAKTISTMRRRIKLLAFLF 62

Query: 61  EEVHESNSPQLPPSSLVCFTELFSVVRRVKLLIQSCEEGSCLWSLLQTELISNQFYQLVK 120
           EEV ESNSP LPPSS++C TE+FSV+RRVK+L QSCEEGSCLWSLLQTE ISNQFYQ VK
Sbjct: 63  EEVQESNSP-LPPSSILCLTEIFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFVK 122

Query: 121 EIGRVLDILPLSFLKLTDDTREQVELLHNQAKRFEFSADEREVRRRDELLELMMNK---- 180
           EIGRVLDILPLS LKLTDDTREQVELLH+QAKRFEFS + REV+RRDELL+LM NK    
Sbjct: 123 EIGRVLDILPLSLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDELLQLMSNKERNY 182

Query: 181 KNKDLA------EFFSSVGLRSLMDCDEEISKLEVEALKQAGTGGIMVVSNINNLISLVR 240
           KNK L       E FSSVGLR++MDCDEEISKLE EALKQAGTGGI+VVSNINNLISLV+
Sbjct: 183 KNKGLGEVGKIKEIFSSVGLRNMMDCDEEISKLEAEALKQAGTGGIIVVSNINNLISLVK 242

Query: 241 HAKTVLFSKTE------QFNLKFQH------HSCSSISMDQIPDDFRCPISLDVMRDPVI 300
           HAKTV+FSK E      ++NLKFQH      HS SS S+ QIPDDFRCPISLD MRDPVI
Sbjct: 243 HAKTVIFSKKENENDGGKYNLKFQHSNKHLDHSSSSNSLVQIPDDFRCPISLDFMRDPVI 302

Query: 301 VSSGHTYDRNSIAQWIDSGHHVCPKSNQRLIHVALIPNYALKSLMQQWCQENNVNLFEPT 360
           +SSGHTYDR SIAQWIDSGHHVCPKSNQRLIH+ALIPNYALKSLMQQWCQENN+N+ EPT
Sbjct: 303 ISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALKSLMQQWCQENNINMNEPT 362

Query: 361 KPCSF--LKRSNSRRYRSEEPIDQISASKAASDAVKMTAEFLVGKLATGSPDVQRQAAYE 420
           KP S   L+RSNS++Y SEEP+D ISASKAASDAVKMTAEFLVGKLATGSPD+QRQAAYE
Sbjct: 363 KPYSSFGLERSNSKKYLSEEPVDHISASKAASDAVKMTAEFLVGKLATGSPDIQRQAAYE 422

Query: 421 LRLLAKTGMDSRRMIAEAGAIPFLVTLLRSGDPRIEENAVTALFNLAIFNNNKILIVAAG 480
           LRLLAKTGMD+RRMIAEAGAIPFLVTLL+SGDPRIEENAVTALFNLAIFNNNKILIVAAG
Sbjct: 423 LRLLAKTGMDNRRMIAEAGAIPFLVTLLKSGDPRIEENAVTALFNLAIFNNNKILIVAAG 482

Query: 481 ALDNITHILESGKTMEARENAAATIFSLTMVDEFKISIGASPKAIPALVRLLKEGNSAGK 540
           A+DNITHILESGKTMEARENAAATI+SLTMVDEFKI+IGASPKAIPALVRLLKEGNSAGK
Sbjct: 483 AIDNITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPKAIPALVRLLKEGNSAGK 542

Query: 541 RDAATALCNLALYKANKACIVGAGAVPLLIELLTDEKAGITDDALQALSLVLGCSEGLQE 600
           RDAATALCNLALY ANKACIV AGAVPLLIELLTD+KAGITDDALQALSLVLGCSEGLQE
Sbjct: 543 RDAATALCNLALYNANKACIVVAGAVPLLIELLTDDKAGITDDALQALSLVLGCSEGLQE 602

Query: 601 IRKSRVLVPLLVDLLRFGSPNGKESSITVLLGMCKDGGEEVGRRLLMNPRSIPSLQSLAA 660
           IRKSRVLV LL+DLLRFGSP GK+SS+T+LLG+CKDGGEEV RRLL+NPRSIPSLQSLAA
Sbjct: 603 IRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEVARRLLINPRSIPSLQSLAA 662

Query: 661 DGSLKARRKADALLRLLNRCCFQSQPC 664
           DGSLKARRKADALLRLLNRCCFQSQPC
Sbjct: 663 DGSLKARRKADALLRLLNRCCFQSQPC 688

BLAST of Carg05967 vs. TrEMBL
Match: tr|A0A0A0K7R8|A0A0A0K7R8_CUCSA (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G012250 PE=4 SV=1)

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 570/687 (82.97%), Postives = 620/687 (90.25%), Query Frame = 0

Query: 1   MDVALSSPIMAASAFLPHGSVLQSLIVLANEVCLEEKASFVQARTVSTMRRRIKLLAFLF 60
           MDVALSS + A+S+  P GS+L+SLI+L+NEV  EEKA FV A+T+STMRRRIKLLAFLF
Sbjct: 3   MDVALSSMMAASSSLFPSGSLLESLILLSNEVAFEEKAPFVHAKTISTMRRRIKLLAFLF 62

Query: 61  EEVHESNSPQLPPSSLVCFTELFSVVRRVKLLIQSCEEGSCLWSLLQTELISNQFYQLVK 120
           EEV ESNSP LPPSS++C TE+FSV+RRVK+L QSCEEGSCLWSLLQTE ISNQFYQ VK
Sbjct: 63  EEVQESNSP-LPPSSILCLTEVFSVIRRVKILTQSCEEGSCLWSLLQTESISNQFYQFVK 122

Query: 121 EIGRVLDILPLSFLKLTDDTREQVELLHNQAKRFEFSADEREVRRRDELLELMMNK---- 180
           EIGRVLDILPLS LKLTDDTREQVELLH+QAKRFEFS + REV+RRDELL+LM NK    
Sbjct: 123 EIGRVLDILPLSLLKLTDDTREQVELLHSQAKRFEFSVNAREVQRRDELLQLMSNKERNY 182

Query: 181 KNKDLA------EFFSSVGLRSLMDCDEEISKLEVEALKQAGTGGIMVVSNINNLISLVR 240
           K K L       E FSSVGLR++MDCDEEI+KLE E LKQAGTGGI+VVSNINNLISLV+
Sbjct: 183 KTKGLGEVGKIKEIFSSVGLRNMMDCDEEITKLEAEGLKQAGTGGIIVVSNINNLISLVK 242

Query: 241 HAKTVLFSKTE------QFNLKFQH------HSCSSISMDQIPDDFRCPISLDVMRDPVI 300
           HAKTV+FSK E      ++NLKF H      HS SS S+ QIPDDFRCPISLD MRDPVI
Sbjct: 243 HAKTVIFSKKENENDGGKYNLKFLHSNKHLDHSSSSNSLVQIPDDFRCPISLDFMRDPVI 302

Query: 301 VSSGHTYDRNSIAQWIDSGHHVCPKSNQRLIHVALIPNYALKSLMQQWCQENNVNLFEPT 360
           +SSGHTYDR SIAQWIDSGHHVCPKSNQRLIH+ALIPNYAL+SLMQQWCQENN+N+ EPT
Sbjct: 303 ISSGHTYDRYSIAQWIDSGHHVCPKSNQRLIHMALIPNYALRSLMQQWCQENNINMNEPT 362

Query: 361 KPCSF--LKRSNSRRYRSEEPIDQISASKAASDAVKMTAEFLVGKLATGSPDVQRQAAYE 420
           KP S   L+RSNS+RY SEEP+D ISASKAASDA+KMTAEFLVGKLATGSPD+QRQAAYE
Sbjct: 363 KPYSSFELERSNSKRYLSEEPVDHISASKAASDAIKMTAEFLVGKLATGSPDIQRQAAYE 422

Query: 421 LRLLAKTGMDSRRMIAEAGAIPFLVTLLRSGDPRIEENAVTALFNLAIFNNNKILIVAAG 480
           LRLLAKTGMD+RRMIAEAGAIPFLVTLL+SGDPRIEENAVTALFNLAIFNNNKILIVAAG
Sbjct: 423 LRLLAKTGMDNRRMIAEAGAIPFLVTLLKSGDPRIEENAVTALFNLAIFNNNKILIVAAG 482

Query: 481 ALDNITHILESGKTMEARENAAATIFSLTMVDEFKISIGASPKAIPALVRLLKEGNSAGK 540
           A+DNITHILESGKTMEARENAAATI+SLTMVDEFKI+IGASPKAIPALVRLLKEGNSAGK
Sbjct: 483 AIDNITHILESGKTMEARENAAATIYSLTMVDEFKITIGASPKAIPALVRLLKEGNSAGK 542

Query: 541 RDAATALCNLALYKANKACIVGAGAVPLLIELLTDEKAGITDDALQALSLVLGCSEGLQE 600
           RDAATALCNLALY ANKACIV +GAVPLLIELLTD+KAGITDDALQALSLVLGCSEGLQE
Sbjct: 543 RDAATALCNLALYNANKACIVVSGAVPLLIELLTDDKAGITDDALQALSLVLGCSEGLQE 602

Query: 601 IRKSRVLVPLLVDLLRFGSPNGKESSITVLLGMCKDGGEEVGRRLLMNPRSIPSLQSLAA 660
           IRKSRVLV LL+DLLRFGSP GK+SS+T+LLG+CKDGGEEV RRLL+NPRSIPSLQSLAA
Sbjct: 603 IRKSRVLVSLLIDLLRFGSPKGKDSSLTLLLGLCKDGGEEVARRLLINPRSIPSLQSLAA 662

Query: 661 DGSLKARRKADALLRLLNRCCFQSQPC 664
           DGSLKARRKADALLRLLNRCC QSQPC
Sbjct: 663 DGSLKARRKADALLRLLNRCCSQSQPC 688

BLAST of Carg05967 vs. TrEMBL
Match: tr|A0A2J6KJG9|A0A2J6KJG9_LACSA (RING-type E3 ubiquitin transferase OS=Lactuca sativa OX=4236 GN=LSAT_2X123761 PE=4 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 3.9e-236
Identity = 440/682 (64.52%), Postives = 544/682 (79.77%), Query Frame = 0

Query: 1   MDVALSSPIMAASAFLPHGSVLQSLIVLANEVCLEEKASFVQARTVSTMRRRIKLLAFLF 60
           MDV L  P+M +  FLP  S+L+SLI ++NEV   EK    Q++ VSTM RRIKLL+ LF
Sbjct: 1   MDVTLPPPLMISQGFLPTDSLLESLIQISNEVTTIEKIPITQSKNVSTMIRRIKLLSSLF 60

Query: 61  EEVHESNSPQLPPSSLVCFTELFSVVRRVKLLIQSCEEGSCLWSLLQTELISNQFYQLVK 120
           EE+ E+N+ QLPPSS++C TEL+SV+RRVK LIQ+CE+GSCLW+L++ E ISNQF+ +VK
Sbjct: 61  EEIQETNT-QLPPSSILCLTELYSVIRRVKSLIQACEQGSCLWNLVRAESISNQFHVIVK 120

Query: 121 EIGRVLDILPLSFLKLTDDTREQVELLHNQAKRFEFSADEREVRRRDELLELMM-----N 180
           E+ R LDILPLS LK+  DTREQVELLH Q KR +   D +EV+RR+ELL++MM      
Sbjct: 121 EMSRALDILPLSLLKIGADTREQVELLHRQVKRVDLMTDSKEVQRREELLQVMMASTKSK 180

Query: 181 KKNKDLAEF------FSSVGLRSLMDCDEEISKLEVEALKQAGTGGIMVVSNINNLISLV 240
            ++K + +F       + +GL++  D +EEISKLE EA KQAGTGG++VVSNINNLISLV
Sbjct: 181 SRSKAVIDFEKLKVIMTRIGLKTPFDYEEEISKLEAEAEKQAGTGGLIVVSNINNLISLV 240

Query: 241 RHAKTVLFSK-------TEQFNLKFQHHSCSSISMDQIPDDFRCPISLDVMRDPVIVSSG 300
             +K+++FSK        E  N + + +S S   +  IPD++RCPISLD+MRDPVIV+SG
Sbjct: 241 SLSKSIVFSKHDHAIAQEEDKNSQSRRYSFSQSMVINIPDEYRCPISLDLMRDPVIVASG 300

Query: 301 HTYDRNSIAQWIDSGHHVCPKSNQRLIHVALIPNYALKSLMQQWCQENNVNLFE----PT 360
           HTYDR SIAQWI+SGHH CPKS +RLIH+ALIPNYALKSL+ Q+C ENN+ + E     +
Sbjct: 301 HTYDRVSIAQWINSGHHTCPKSGKRLIHMALIPNYALKSLIHQYCDENNLPVDENAPATS 360

Query: 361 KPCSFLKRSNSRRYRSEEPIDQISASKAASDAVKMTAEFLVGKLATGSPDVQRQAAYELR 420
              SF K  + +R   E+ ID ISA+K+A DAVKMTAEFLVGKLA GS D+QRQAAYELR
Sbjct: 361 STHSFEKNPSVKRKLPEKAIDHISATKSAMDAVKMTAEFLVGKLAMGSVDIQRQAAYELR 420

Query: 421 LLAKTGMDSRRMIAEAGAIPFLVTLLRSGDPRIEENAVTALFNLAIFNNNKILIVAAGAL 480
           LLAKTGMD+RR+I+EAGAIPFLVTLL S DPRI+E+AVT L NL+IF NNK+LIVAAGAL
Sbjct: 421 LLAKTGMDNRRLISEAGAIPFLVTLLSSHDPRIQEHAVTTLLNLSIFENNKLLIVAAGAL 480

Query: 481 DNITHILESGKTMEARENAAATIFSLTMVDEFKISIGASPKAIPALVRLLKEGNSAGKRD 540
           D+I  +L SGKTMEARENAAA IFSL+++D++K+ IG+ PKAIP L+ LLK+G + GKRD
Sbjct: 481 DSIVDVLGSGKTMEARENAAAAIFSLSLIDDYKVVIGSRPKAIPYLIGLLKDGTTTGKRD 540

Query: 541 AATALCNLALYKANKACIVGAGAVPLLIELLTDEKAGITDDALQALSLVLGCSEGLQEIR 600
           AATAL NLA+Y  NK  +V +GA+P+LI+LL D+KAGITDDAL  LSL+LGC EG  EIR
Sbjct: 541 AATALINLAVYSVNKVNVVVSGAIPVLIDLLMDDKAGITDDALAVLSLLLGCCEGRDEIR 600

Query: 601 KSRVLVPLLVDLLRFGSPNGKESSITVLLGMCKDGGEEVGRRLLMNPRSIPSLQSLAADG 660
           KS+VLVP+L+DLLRFGSP GKE+SIT+LLG+CKDGG+EV RRLLMNPRSIPSLQSLAADG
Sbjct: 601 KSKVLVPILIDLLRFGSPKGKENSITLLLGLCKDGGDEVARRLLMNPRSIPSLQSLAADG 660

BLAST of Carg05967 vs. TrEMBL
Match: tr|T1R384|T1R384_DATGL (RING-type E3 ubiquitin transferase OS=Datisca glomerata OX=34297 GN=PUB1 PE=2 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 2.4e-233
Identity = 445/671 (66.32%), Postives = 517/671 (77.05%), Query Frame = 0

Query: 10  MAASAFLPHGSVLQSLIVLANEVCLEEKASFVQARTVSTMRRRIKLLAFLFEEVHESNSP 69
           MA+S FLP GS+L+SLI ++NEV   E   FVQ R +STMRRRIKLL+FLFEE+ E+NSP
Sbjct: 1   MASSGFLPTGSLLESLIHISNEVASMENMPFVQVRNISTMRRRIKLLSFLFEEIQEANSP 60

Query: 70  QLPPSSLVCFTELFSVVRRVKLLIQSCEEGSCLWSLLQTELISNQFYQLVKEIGRVLDIL 129
            LPPSS++CFTELFSV+RRVK LIQ C+EGS LW+LLQTE I+NQFY LVKE+ R LDIL
Sbjct: 61  -LPPSSILCFTELFSVIRRVKFLIQGCKEGSSLWNLLQTEFITNQFYVLVKEMVRALDIL 120

Query: 130 PLSFLKLTDDTREQVELLHNQAKRFEFSADEREVRRRDELLELM-----MNKKNK----- 189
           PLS LK+T D REQVELLHNQAK+ E S D ++++RR+EL+++M      N +NK     
Sbjct: 121 PLSLLKITADVREQVELLHNQAKQVELSVDPQDLQRREELVQVMTKNNEKNSRNKGFIEI 180

Query: 190 -DLAEFFSSVGLRSLMDCDEEISKLEVEALKQAGTGGIMVVSNINNLISLVRHAKTVLFS 249
             + E  S++GLRS++D ++EI+KLE EALKQAGTGG++VVSNINNLISLV ++K++LFS
Sbjct: 181 GKVKEILSNIGLRSIVDYEDEITKLEAEALKQAGTGGLIVVSNINNLISLVLYSKSMLFS 240

Query: 250 --------KTEQFNLKFQHHSCSSISMDQIPDDFRCPISLDVMRDPVIVSSGHTYDRNSI 309
                   K +  N +F  HS SS S+  IPD+FRCPISLD+M+DPVI++SG TYDR SI
Sbjct: 241 YGDDDKSVKDQSSNKRFD-HSSSSKSLVPIPDEFRCPISLDLMKDPVIIASGQTYDRTSI 300

Query: 310 AQWIDSGHHVCPKSNQRLIHVALIPNYALKSLMQQWCQENNVNLFEPTKPCSFLKRSNSR 369
           AQWIDSGHHVCPKS QRLIH+ALIPNYALKSL+QQWCQENNV L EP    S L+RSNSR
Sbjct: 301 AQWIDSGHHVCPKSGQRLIHMALIPNYALKSLVQQWCQENNVQLNEPATASSELERSNSR 360

Query: 370 RYRSEEPIDQISASKAASDAVKMTAEFLVGKLATGSPDVQRQAAYELRLLAKTGMDSRRM 429
           R  SE  +D IS +KAA DAVKMTAEFLVGKLATGS D+QRQAAYE+RLLAKTGMD+RRM
Sbjct: 361 RKLSENAVDHISVAKAAIDAVKMTAEFLVGKLATGSSDIQRQAAYEIRLLAKTGMDNRRM 420

Query: 430 IAEAGAIPFLVTLLRSGDPRIEENAVTALFNLAIFNNNKILIVAAGALDNITHILESGKT 489
           IAEAGAIPFLVTLL S DPR++ENAVTAL NL+IFNNNK LI++AGA+DNI H+LESGKT
Sbjct: 421 IAEAGAIPFLVTLLTSTDPRLQENAVTALLNLSIFNNNKTLIISAGAIDNIIHVLESGKT 480

Query: 490 MEARENAAATIFSLTMVDEFKISIGASPKAIPALVRLLKEGNSAGKRDAATALCNLALYK 549
            E                                                        Y 
Sbjct: 481 TEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYS 540

Query: 550 ANKACIVGAGAVPLLIELLTDEKAGITDDALQALSLVLGCSEGLQEIRKSRVLVPLLVDL 609
           ANKA +V AGAVPLLI+LLTD+KAGITDDALQ LSLVLGCS+GLQEIRKSRVLVPLL+DL
Sbjct: 541 ANKARVVVAGAVPLLIDLLTDDKAGITDDALQVLSLVLGCSDGLQEIRKSRVLVPLLIDL 600

Query: 610 LRFGSPNGKESSITVLLGMCKDGGEEVGRRLLMNPRSIPSLQSLAADGSLKARRKADALL 662
           LRFGS  GKE+SIT+LLG+CKDGGEEV RRLLMNPRSIPSLQSLAADGSLKARRKADALL
Sbjct: 601 LRFGSLKGKENSITLLLGLCKDGGEEVARRLLMNPRSIPSLQSLAADGSLKARRKADALL 660

BLAST of Carg05967 vs. TrEMBL
Match: tr|A0A0L9UWG3|A0A0L9UWG3_PHAAN (RING-type E3 ubiquitin transferase OS=Phaseolus angularis OX=3914 GN=LR48_Vigan07g058300 PE=4 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 1.4e-230
Identity = 443/678 (65.34%), Postives = 522/678 (76.99%), Query Frame = 0

Query: 7   SPIMAASAFLPHGSVLQSLIVLANEVCLEEKASFVQARTVSTMRRRIKLLAFLFEEVHES 66
           S +M +   LP GS+L SLI ++ EV   EK  FV  R VS+M RRIKLL+ LFEE+ ES
Sbjct: 6   SAMMVSQGLLPSGSLLDSLIHISKEVDSMEKFPFVHIRNVSSMIRRIKLLSSLFEEIKES 65

Query: 67  NSPQLPPSSLVCFTELFSVVRRVKLLIQSCEEGSCLWSLLQTELISNQFYQLVKEIGRVL 126
            +P LPPSS++C TELFSV+RRVK+LIQ C++GS LWSL+Q E ISNQFY LVKE+GR L
Sbjct: 66  GTP-LPPSSVLCLTELFSVIRRVKVLIQDCKDGSSLWSLIQLEFISNQFYVLVKEMGRAL 125

Query: 127 DILPLSFLKLTDDTREQVELLHNQAKRFEFSADEREVRRRDELLELMMN-----KKNKDL 186
           DILPLS L +T D REQVELLH QAK+ E   D RE+ RR++L   M N     KKNK  
Sbjct: 126 DILPLSLLNVTSDIREQVELLHKQAKKAELFIDPRELHRREQLKLAMANNSLQKKKNKGF 185

Query: 187 AEF------FSSVGLRSLMDCDEEISKLEVEALKQAGTGGIMVVSNINNLISLVRHAKTV 246
            EF       SS+GLR+  D D EISKL+ EA  QAGTGG+++VSNINNLISLV ++KT+
Sbjct: 186 VEFGKVEEILSSIGLRTPSDYDGEISKLKAEAQNQAGTGGLIIVSNINNLISLVSYSKTM 245

Query: 247 LF-----SKTEQ---------FNLKFQHHSCSSISMDQIPDDFRCPISLDVMRDPVIVSS 306
           +F     S+ E+         +N      S S      +PD+FRCPI+LD+MRDPVIVSS
Sbjct: 246 IFRDGESSRNEEDCKPLSSFLYNKVHDSSSSSQSMTPNVPDEFRCPITLDLMRDPVIVSS 305

Query: 307 GHTYDRNSIAQWIDSGHHVCPKSNQRLIHVALIPNYALKSLMQQWCQENNVNLFEPTKPC 366
           GHTYDR SI QWI+SGHH CPKS QRLIH ALIPNYALKSL+QQWC +NNV + +P+   
Sbjct: 306 GHTYDRTSIGQWINSGHHTCPKSGQRLIHTALIPNYALKSLVQQWCYDNNVPVNDPSTEG 365

Query: 367 SFLKRSNSRRYRSEEPIDQISASKAASDAVKMTAEFLVGKLATGSPDVQRQAAYELRLLA 426
           +   + NS+    EE ID ISA+KAA+DAVKMTAEFLVGKLATGS D+QRQAAYELRLLA
Sbjct: 366 NSHNKKNSK----EEAIDHISANKAAADAVKMTAEFLVGKLATGSADIQRQAAYELRLLA 425

Query: 427 KTGMDSRRMIAEAGAIPFLVTLLRSGDPRIEENAVTALFNLAIFNNNKILIVAAGALDNI 486
           KTGM +R +IAE GAIPFLVTLL S D RI+E+AVT LFNL+IF+NNKILI+AAGA+D+I
Sbjct: 426 KTGMVNRSVIAEVGAIPFLVTLLGSQDSRIQEHAVTTLFNLSIFDNNKILIMAAGAVDSI 485

Query: 487 THILESGKTMEARENAAATIFSLTMVDEFKISIGASPKAIPALVRLLKEGNSAGKRDAAT 546
             +LESGKTMEARENAAA I+SL MVDE K+ IG  P+AIPALV LLKEG S GK+DAA+
Sbjct: 486 VEVLESGKTMEARENAAAAIYSLCMVDELKVHIGGRPRAIPALVGLLKEGTSIGKKDAAS 545

Query: 547 ALCNLALYKANKACIVGAGAVPLLIELLTDEKAGITDDALQALSLVLGCSEGLQEIRKSR 606
           AL NLA+Y  NK   V AGAV +L+ELL D+KAGITDDAL  L+ +LGCSEGLQEIR SR
Sbjct: 546 ALFNLAVYNPNKVSAVKAGAVSVLVELLMDDKAGITDDALAVLAALLGCSEGLQEIRNSR 605

Query: 607 VLVPLLVDLLRFGSPNGKESSITVLLGMCKDGGEEVGRRLLMNPRSIPSLQSLAADGSLK 660
            LVPLL+DLLRFGS  GKE+SIT+LLG+CK+ GE V RRLL NPRSIPSLQSLA+DGSL+
Sbjct: 606 ALVPLLIDLLRFGSVKGKENSITLLLGLCKEEGEVVARRLLANPRSIPSLQSLASDGSLR 665

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022963960.10.0e+0099.70U-box domain-containing protein 1-like [Cucurbita moschata][more]
XP_022990647.10.0e+0097.13U-box domain-containing protein 1-like [Cucurbita maxima][more]
XP_023535888.10.0e+0096.83U-box domain-containing protein 1-like [Cucurbita pepo subsp. pepo][more]
XP_023521656.10.0e+0096.82U-box domain-containing protein 1-like, partial [Cucurbita pepo subsp. pepo][more]
XP_008441478.10.0e+0083.84PREDICTED: U-box domain-containing protein 13-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT5G01830.12.0e-8433.64ARM repeat superfamily protein[more]
AT1G29340.14.4e-8433.14plant U-box 17[more]
AT1G60190.12.5e-7129.38ARM repeat superfamily protein[more]
AT1G10560.11.2e-6027.07plant U-box 18[more]
AT3G46510.11.1e-5835.15plant U-box 13[more]
Match NameE-valueIdentityDescription
sp|E4NKF8|PUB1_MEDTR1.4e-18855.15U-box domain-containing protein 1 OS=Medicago truncatula OX=3880 GN=PUB1 PE=1 SV... [more]
sp|Q9LZW3|PUB16_ARATH3.6e-8333.64U-box domain-containing protein 16 OS=Arabidopsis thaliana OX=3702 GN=PUB16 PE=2... [more]
sp|Q9C7R6|PUB17_ARATH7.9e-8333.14U-box domain-containing protein 17 OS=Arabidopsis thaliana OX=3702 GN=PUB17 PE=2... [more]
sp|O80742|PUB19_ARATH4.5e-7029.38U-box domain-containing protein 19 OS=Arabidopsis thaliana OX=3702 GN=PUB19 PE=2... [more]
sp|Q6EUK7|PUB04_ORYSJ1.5e-6530.08U-box domain-containing protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=PU... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3B3K6|A0A1S3B3K6_CUCME0.0e+0083.84RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485586 PE=4 ... [more]
tr|A0A0A0K7R8|A0A0A0K7R8_CUCSA0.0e+0082.97RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G012250 PE... [more]
tr|A0A2J6KJG9|A0A2J6KJG9_LACSA3.9e-23664.52RING-type E3 ubiquitin transferase OS=Lactuca sativa OX=4236 GN=LSAT_2X123761 PE... [more]
tr|T1R384|T1R384_DATGL2.4e-23366.32RING-type E3 ubiquitin transferase OS=Datisca glomerata OX=34297 GN=PUB1 PE=2 SV... [more]
tr|A0A0L9UWG3|A0A0L9UWG3_PHAAN1.4e-23065.34RING-type E3 ubiquitin transferase OS=Phaseolus angularis OX=3914 GN=LR48_Vigan0... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005488binding
GO:0005515protein binding
GO:0004842ubiquitin-protein transferase activity
Vocabulary: Biological Process
TermDefinition
GO:0016567protein ubiquitination
Vocabulary: INTERPRO
TermDefinition
IPR016024ARM-type_fold
IPR013083Znf_RING/FYVE/PHD
IPR011989ARM-like
IPR000225Armadillo
IPR003613Ubox_domain
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0005488 binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05967-RACarg05967-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 263..326
e-value: 8.5E-30
score: 115.0
IPR003613U box domainPFAMPF04564U-boxcoord: 260..331
e-value: 1.5E-20
score: 73.0
IPR003613U box domainPROSITEPS51698U_BOXcoord: 259..333
score: 38.109
IPR000225ArmadilloSMARTSM00185arm_5coord: 488..528
e-value: 9.6
score: 13.4
coord: 445..486
e-value: 110.0
score: 5.1
coord: 570..611
e-value: 34.0
score: 9.1
coord: 404..444
e-value: 7.5E-5
score: 32.1
coord: 529..569
e-value: 0.93
score: 18.5
IPR000225ArmadilloPFAMPF00514Armcoord: 532..565
e-value: 8.3E-5
score: 22.4
coord: 408..443
e-value: 7.8E-8
score: 32.0
coord: 497..527
e-value: 2.0E-5
score: 24.3
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 499..541
score: 10.552
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 415..457
score: 12.827
IPR011989Armadillo-like helicalGENE3DG3DSA:1.25.10.10coord: 376..494
e-value: 3.0E-23
score: 84.0
IPR011989Armadillo-like helicalGENE3DG3DSA:1.25.10.10coord: 495..659
e-value: 5.0E-21
score: 77.1
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 241..336
e-value: 2.1E-28
score: 100.2
NoneNo IPR availablePANTHERPTHR23315:SF132SUBFAMILY NOT NAMEDcoord: 10..661
NoneNo IPR availablePANTHERPTHR23315BETA CATENIN-RELATED ARMADILLO REPEAT-CONTAININGcoord: 10..661
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 264..306
e-value: 1.74877E-23
score: 93.4522
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 252..336
IPR016024Armadillo-type foldSUPERFAMILYSSF48371ARM repeatcoord: 375..653