Cucsa.132950 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.132950
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionProlyl 4-hydroxylase, alpha subunit
Locationscaffold01037 : 772166 .. 773885 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGTTCTCTGGATTCATTTCTTGTGGGATGTGTATCATCAACTCTGATTTTGCTTTTGATTTTGGCTTTGGCTTTGATTTTGGTAATTTTTTGTTGTTCTAATGATGGGACGAGGAAGAATAATAGAAGGGGTCCGTGGGTTGATCAGTTCGTTTCTTGGGAGCCTAGAGCTTTCATTTGCCACAATTTCCTGGCAAGTTCTTACAATCTCAGCTTCAAATTATGTTCGAATGTTTCTAGTATATACTATATACGTTTTTGTATTTGCTGCTTCATTGTTCGATTTCTTAACAATATGTTTGAACATGCTTTTTGTTAAACAGTCAGAGAAAGAATGCTTGCAGTTGATTAATCTTGCAAAACCTCACATGAGGAGATCAAAATTTATATGATACCACACTTCGCAAGAATGTAACGACAAGGTCTAGATTTTTGTGTCACTTGGTTAGACTAATGCGTAGGACTCGTGTGATCCTTGCACAAGGGCGGCCACCACCAAGTCAGGACTTTGTAGGGGCAAATGACAGGTCATGAGGTGCAAAATGAAAAATGATCAATACAAATGCAGGTCGGATATTGAATATTGAAGAAGGGGgCGAGACGGTGTTCCCGGCTGCGAACAAATGCGTCAGCTCTGTGCCATGGTGGAAGAAATTGCCTACACATGGTAAAGATGGACTCTCTATAAAACCAAaGATGGGAGATGCATTATTTTtCTGGAGCATGAAACCTGATGGTACATTGGATTATACGAGTTTGCATGGTTAGTGATATGTTTACAACACCAAAATCTTACATATTTTAACCATGTTAAAAGAGTTTTACAATGATTTTGTTTCTATTCTCAAATATTGTTTTTGTTAACTATTTATTTGCGAGTGTCCATTACATACTTCCAATATGTTGTCTGATATATGTATAGGTTCCTACCCCGTTATAAGAGGGGACGAATGGGCATGTGTAAAGCTCATGGATGATTAGAACACAAAACTTAACTGAGGTAAGTTATACAATTCTTTAGATCATCTATACTTTTCCAAATTGGTCCAATTTTAAATTTCCTTTTCTATCTGTACCAATATAATTCTCATCTAGAACCTCAATATATTAAGTTAAAGAAGGTGCATGGATATATTTCCAGAACTTAAATCATGCAATAAATATTTAAAATAAAAATATAGTACTAAAAGGTAAGGAATATACATTATGTTTTTtGTTTCCGAGTTTATAGATAATAGGTCAATTTTGTAACACAATCAAAGTTTAAGAAATAGTGATGCATGTCTCATATAAGTACTTTGATGTAATCAAATTGCAGGGTAATCGAACCACAAGTATTAGTGGAGAAGATGAGAAGAGACTCACATTTTTTTTTTtAGATATATGATCGAGAATCAGTTTTGTTGATTAGTTACTCTATATCGAGATATATATAGGATCAATTTTGATTTATTTTTGAATCTTTTTATATATTGATCCATTTCTGTTTGGTTATGGATAAACATGGTTTCGATAATTTGACAAAAAAaGTTTATGATAAAACCTTTGTATTTATGGACATTTTTATCATACAAAAAATTCTAAATACTTAACCACCTCCCAAATATAATGTTAAAATGAAAATCTAGAAAGCCATATTtCTTTTTTTTTTttttGTTtGAACAATAAATAAGAAGTGTGGAAGCAGGATACTATTTCCAAGAAAATATAATATATAA

mRNA sequence

ATGCGTTCTCTGGATTCATTTCTTGTGGGATGTGTATCATCAACTCTGATTTTGCTTTTGATTTTGGCTTTGGCTTTGATTTTGGTAATTTTTTGTTGTTCTAATGATGGGACGAGGAAGAATAATAGAAGGGGTCCGTGGGTTGATCAGTTCGTTTCTTGGGAGCCTAGAGCTTTCATTTGCCACAATTTCCTGGCAAGTCGGATATTGAATATTGAAGAAGGGGGCGAGACGGTGTTCCCGGCTGCGAACAAATGCGTCAGCTCTGTGCCATGGTGGAAGAAATTGCCTACACATGGTAAAGATGGACTCTCTATAAAACCAAAGATGGGAGATGCATTATTTTTCTGGAGCATGAAACCTGATGGTACATTGGATTATACGAGTTTGCATGGTTCCTACCCCGTTATAAGAGGGGACGAATGGTGTGGAAGCAGGATACTATTTCCAAGaaaatataatatataa

Coding sequence (CDS)

ATGCGTTCTCTGGATTCATTTCTTGTGGGATGTGTATCATCAACTCTGATTTTGCTTTTGATTTTGGCTTTGGCTTTGATTTTGGTAATTTTTTGTTGTTCTAATGATGGGACGAGGAAGAATAATAGAAGGGGTCCGTGGGTTGATCAGTTCGTTTCTTGGGAGCCTAGAGCTTTCATTTGCCACAATTTCCTGGCAAGTCGGATATTGAATATTGAAGAAGGGGgCGAGACGGTGTTCCCGGCTGCGAACAAATGCGTCAGCTCTGTGCCATGGTGGAAGAAATTGCCTACACATGGTAAAGATGGACTCTCTATAAAACCAAaGATGGGAGATGCATTATTTTtCTGGAGCATGAAACCTGATGGTACATTGGATTATACGAGTTTGCATGGTTCCTACCCCGTTATAAGAGGGGACGAATGGTGTGGAAGCAGGATACTATTTCCAAGAAAATATAATATATAA

Protein sequence

MRSLDSFLVGCVSSTLILLLILALALILVIFCCSNDGTRKNNRRGPWVDQFVSWEPRAFICHNFLASRILNIEEGGETVFPAANKCVSSVPWWKKLPTHGKDGLSIKPKMGDALFFWSMKPDGTLDYTSLHGSYPVIRGDEWCGSRILFPRKYNI*
Homology
[Close]
BLAST of Cucsa.132950 vs. Swiss-Prot
Match: P4H3_ARATH (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana GN=P4H3 PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.1e-24
Identity = 52/85 (61.18%), Postives = 65/85 (76.47%), Query Frame = 1 

Query: 71  NIEEGGETVFPAANKCVSSVPWWKKLPTHGKDGLSIKPKMGDALFFWSMKPDGTLDYTSL 130
           ++EEGGETVFPAAN   SSVPW+ +L   GK GLS+KP+MGDAL FWSM+PD TLD TSL
Sbjct: 203 DVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVKPRMGDALLFWSMRPDATLDPTSL 262

Query: 131 HGSYPVIRGDEWCGSRILFPRKYNI 156
           HG  PVIRG++W  ++ +   +Y I
Sbjct: 263 HGGCPVIRGNKWSSTKWMHVGEYKI 287


HSP 2 Score: 33.9 bits (76), Expect = 1.9e+00
Identity = 13/24 (54.17%), Postives = 18/24 (75.00%), Query Frame = 1 

Query: 43 RRGPWVDQFVSWEPRAFICHNFLA 67
          +RG    + +SWEPRAF+ HNFL+
Sbjct: 70 KRGDQWTEVLSWEPRAFVYHNFLS 93

[Close]
BLAST of Cucsa.132950 vs. Swiss-Prot
Match: P4H10_ARATH (Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana GN=P4H10 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 6.2e-23
Identity = 49/85 (57.65%), Postives = 62/85 (72.94%), Query Frame = 1 

Query: 71  NIEEGGETVFPAANKCVSSVPWWKKLPTHGKDGLSIKPKMGDALFFWSMKPDGTLDYTSL 130
           ++EEGGETVFPAA    S+VPWW +L   GK GLS+KPKMGDAL FWSM PD TLD +SL
Sbjct: 205 DVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSVKPKMGDALLFWSMTPDATLDPSSL 264

Query: 131 HGSYPVIRGDEWCGSRILFPRKYNI 156
           HG   VI+G++W  ++ L   +Y +
Sbjct: 265 HGGCAVIKGNKWSSTKWLRVHEYKV 289


HSP 2 Score: 34.3 bits (77), Expect = 1.5e+00
Identity = 24/72 (33.33%), Postives = 36/72 (50.00%), Query Frame = 1 

Query: 12 VSSTLILLLILALALILVIFCCSNDGTRKNN-----------RRGP-------WVDQFVS 66
          + ST ++L++LA  ++ V    +N G+ K N           R G        WV+  +S
Sbjct: 26 IMSTFVILILLAFGILSVP--SNNAGSSKANDLTSIVRKTLQRSGEDDSKNERWVE-IIS 85

[Close]
BLAST of Cucsa.132950 vs. Swiss-Prot
Match: P4H8_ARATH (Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana GN=P4H8 PE=3 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 4.0e-22
Identity = 47/84 (55.95%), Postives = 61/84 (72.62%), Query Frame = 1 

Query: 71  NIEEGGETVFPAANKCVSSVPWWKKLPTHGKDGLSIKPKMGDALFFWSMKPDGTLDYTSL 130
           +++EGGETVFPAA   VS VPWW +L   GK+GLS+ PK  DAL FWSMKPD +LD +SL
Sbjct: 207 DVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLSVLPKKRDALLFWSMKPDASLDPSSL 266

Query: 131 HGSYPVIRGDEWCGSRILFPRKYN 155
           HG  PVI+G++W  ++     +YN
Sbjct: 267 HGGCPVIKGNKWSSTKWFHVHEYN 290


HSP 2 Score: 33.1 bits (74), Expect = 3.3e+00
Identity = 13/31 (41.94%), Postives = 20/31 (64.52%), Query Frame = 1 

Query: 37 GTRKNNRRGPWVDQFVSWEPRAFICHNFLAS 68
          G  ++     W++  +SWEPRAF+ HNFL +
Sbjct: 69 GDEEDGNGDRWLE-VISWEPRAFVYHNFLTN 98

[Close]
BLAST of Cucsa.132950 vs. Swiss-Prot
Match: P4H5_ARATH (Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana GN=P4H5 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 2.9e-20
Identity = 40/85 (47.06%), Postives = 62/85 (72.94%), Query Frame = 1 

Query: 71  NIEEGGETVFPAANKCVSSVPWWKKLPTHGKDGLSIKPKMGDALFFWSMKPDGTLDYTSL 130
           ++++GGETVFPAA   +S+VPWW +L   GK+GLS+ PK  DAL FW+M+PD +LD +SL
Sbjct: 207 DVDDGGETVFPAARGNISAVPWWNELSKCGKEGLSVLPKKRDALLFWNMRPDASLDPSSL 266

Query: 131 HGSYPVIRGDEWCGSRILFPRKYNI 156
           HG  PV++G++W  ++     ++ +
Sbjct: 267 HGGCPVVKGNKWSSTKWFHVHEFKV 291


HSP 2 Score: 30.8 bits (68), Expect = 1.6e+01
Identity = 12/21 (57.14%), Postives = 16/21 (76.19%), Query Frame = 1 

Query: 47 WVDQFVSWEPRAFICHNFLAS 68
          WV+  +SWEPRA + HNFL +
Sbjct: 79 WVE-VISWEPRAVVYHNFLTN 98

[Close]
BLAST of Cucsa.132950 vs. Swiss-Prot
Match: P4H13_ARATH (Prolyl 4-hydroxylase 13 OS=Arabidopsis thaliana GN=P4H13 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.2e-12
Identity = 37/95 (38.95%), Postives = 56/95 (58.95%), Query Frame = 1 

Query: 71  NIEEGGETVFPAAN-----------KCVSSVPWWKKLPTHGKDGLSIKPKMGDALFFWSM 130
           ++EEGGET+FP  N           KCV               GL +KP+ GDA+FF+++
Sbjct: 195 SVEEGGETMFPFENGRNMNGRYDYEKCV---------------GLKVKPRQGDAIFFYNL 254

Query: 131 KPDGTLDYTSLHGSYPVIRGDEWCGSRILFPRKYN 155
            P+GT+D TSLHGS PVI+G++W  ++ +  + Y+
Sbjct: 255 FPNGTIDQTSLHGSCPVIKGEKWVATKWIRDQTYD 274

[Close]
BLAST of Cucsa.132950 vs. TrEMBL
Match: A0A103Y5K3_CYNCS (Prolyl 4-hydroxylase, alpha subunit OS=Cynara cardunculus var. scolymus GN=Ccrd_018785 PE=4 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 5.3e-29
Identity = 62/115 (53.91%), Postives = 85/115 (73.91%), Query Frame = 1 

Query: 41  NNRRGPWVDQFVSWEPRAFICHNFLASRILNIEEGGETVFPAANKCVSSVPWWKKLPTHG 100
           ++R   WV+  +SWEPRA + HNFL S + ++EEGGETVFP+A   +S+V WW +L   G
Sbjct: 70  DDRADQWVE-VISWEPRAVVYHNFLVS-MSDVEEGGETVFPSAKGNISAVSWWNQLSECG 129

Query: 101 KDGLSIKPKMGDALFFWSMKPDGTLDYTSLHGSYPVIRGDEWCGSRILFPRKYNI 156
           KDGLS+KPKMGDAL FWSMKPD +LD +SLHG  PVI+G++W  ++ +   +Y +
Sbjct: 130 KDGLSVKPKMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWIHVDEYKV 182

[Close]
BLAST of Cucsa.132950 vs. TrEMBL
Match: G7LB32_MEDTR (Prolyl 4-hydroxylase alpha-like protein OS=Medicago truncatula GN=MTR_8g074810 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 8.7e-24
Identity = 53/72 (73.61%), Postives = 61/72 (84.72%), Query Frame = 1 

Query: 71  NIEEGGETVFPAANKCVSSVPWWKKLPTHGKDGLSIKPKMGDALFFWSMKPDGTLDYTSL 130
           ++EEGGETVFPAA   +SSVPWW +L   GK GLSIKPKMGDAL FWSMKPDGTLD +SL
Sbjct: 194 DVEEGGETVFPAAKGNISSVPWWNELSDCGKKGLSIKPKMGDALLFWSMKPDGTLDPSSL 253

Query: 131 HGSYPVIRGDEW 143
           HG+ PVI+GD+W
Sbjct: 254 HGACPVIKGDKW 265

[Close]
BLAST of Cucsa.132950 vs. TrEMBL
Match: G7LB32_MEDTR (Prolyl 4-hydroxylase alpha-like protein OS=Medicago truncatula GN=MTR_8g074810 PE=4 SV=1)

HSP 1 Score: 41.2 bits (95), Expect = 1.4e+00
Identity = 25/60 (41.67%), Postives = 38/60 (63.33%), Query Frame = 1 

Query: 12 VSSTLILLLILALALILV--IFCCSNDGTR-KNNRRGPWVDQFVSWEPRAFICHNFLASR 69
          V+ TL++L++L L +  +  I   S++  R ++N    WV Q VSWEPRAF+ HNFL  +
Sbjct: 28 VTFTLLILILLTLRIPKLNHINSISHNALRSEDNDNKRWV-QIVSWEPRAFLYHNFLTKK 86


HSP 2 Score: 116.7 bits (291), Expect = 2.5e-23
Identity = 53/79 (67.09%), Postives = 64/79 (81.01%), Query Frame = 1 

Query: 72  IEEGGETVFPAANKCVSSV-PWWKKLPTHGKD-GLSIKPKMGDALFFWSMKPDGTLDYTS 131
           ++EGG TVFP A KC SS   WWKKLP +GKD GLS+KPKMGDAL FWS+KPDGTLD TS
Sbjct: 133 VKEGGATVFPEAKKCASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTS 192

Query: 132 LHGSYPVIRGDEWCGSRIL 149
           LH S PV++GD+W G +++
Sbjct: 193 LHASSPVVKGDKWVGVKLM 211

[Close]
BLAST of Cucsa.132950 vs. TrEMBL
Match: A0A0A0LFF5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G009620 PE=4 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.5e-23
Identity = 54/83 (65.06%), Postives = 64/83 (77.11%), Query Frame = 1 

Query: 71  NIEEGGETVFPAANKCVSSVPWWKKLPTHGKDGLSIKPKMGDALFFWSMKPDGTLDYTSL 130
           ++EEGGETVFPAA    SSVPWW +L   GK GLS+KPKMGDAL FWSMKPD TLD TSL
Sbjct: 176 DVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDATLDPTSL 235

Query: 131 HGSYPVIRGDEWCGSRILFPRKY 154
           HG+ PVIRG++W  ++ +   KY
Sbjct: 236 HGACPVIRGNKWSCTKWMHVDKY 258

[Close]
BLAST of Cucsa.132950 vs. TrEMBL
Match: A0A0A0LFF5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G009620 PE=4 SV=1)

HSP 1 Score: 37.4 bits (85), Expect = 2.0e+01
Identity = 18/32 (56.25%), Postives = 23/32 (71.88%), Query Frame = 1 

Query: 35 NDGTRKNNRRGPWVDQFVSWEPRAFICHNFLA 67
          +DG  K  R   WV +F+SWEPRAF+ HNFL+
Sbjct: 38 SDGLGK--RGDQWV-EFISWEPRAFVYHNFLS 66


HSP 2 Score: 116.3 bits (290), Expect = 3.3e-23
Identity = 52/83 (62.65%), Postives = 65/83 (78.31%), Query Frame = 1 

Query: 71  NIEEGGETVFPAANKCVSSVPWWKKLPTHGKDGLSIKPKMGDALFFWSMKPDGTLDYTSL 130
           ++EEGGETVFPAA   +S+VPWW +L   GK+GLSIKPKMGDAL FWSMKPD TLD +SL
Sbjct: 210 DVEEGGETVFPAAKGNISAVPWWNELSQCGKEGLSIKPKMGDALLFWSMKPDATLDPSSL 269

Query: 131 HGSYPVIRGDEWCGSRILFPRKY 154
           HG  PVIRG++W  ++ +   +Y
Sbjct: 270 HGGCPVIRGNKWSSTKWMRVHEY 292

[Close]
BLAST of Cucsa.132950 vs. TAIR10
Match: AT1G20270.1 (AT1G20270.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 114.4 bits (285), Expect = 6.4e-26
Identity = 52/85 (61.18%), Postives = 65/85 (76.47%), Query Frame = 1 

Query: 71  NIEEGGETVFPAANKCVSSVPWWKKLPTHGKDGLSIKPKMGDALFFWSMKPDGTLDYTSL 130
           ++EEGGETVFPAAN   SSVPW+ +L   GK GLS+KP+MGDAL FWSM+PD TLD TSL
Sbjct: 203 DVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVKPRMGDALLFWSMRPDATLDPTSL 262

Query: 131 HGSYPVIRGDEWCGSRILFPRKYNI 156
           HG  PVIRG++W  ++ +   +Y I
Sbjct: 263 HGGCPVIRGNKWSSTKWMHVGEYKI 287


HSP 2 Score: 33.9 bits (76), Expect = 1.1e-01
Identity = 13/24 (54.17%), Postives = 18/24 (75.00%), Query Frame = 1 

Query: 43 RRGPWVDQFVSWEPRAFICHNFLA 67
          +RG    + +SWEPRAF+ HNFL+
Sbjct: 70 KRGDQWTEVLSWEPRAFVYHNFLS 93

[Close]
BLAST of Cucsa.132950 vs. TAIR10
Match: AT5G66060.1 (AT5G66060.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 108.6 bits (270), Expect = 3.5e-24
Identity = 49/85 (57.65%), Postives = 62/85 (72.94%), Query Frame = 1 

Query: 71  NIEEGGETVFPAANKCVSSVPWWKKLPTHGKDGLSIKPKMGDALFFWSMKPDGTLDYTSL 130
           ++EEGGETVFPAA    S+VPWW +L   GK GLS+KPKMGDAL FWSM PD TLD +SL
Sbjct: 205 DVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSVKPKMGDALLFWSMTPDATLDPSSL 264

Query: 131 HGSYPVIRGDEWCGSRILFPRKYNI 156
           HG   VI+G++W  ++ L   +Y +
Sbjct: 265 HGGCAVIKGNKWSSTKWLRVHEYKV 289


HSP 2 Score: 34.3 bits (77), Expect = 8.4e-02
Identity = 24/72 (33.33%), Postives = 36/72 (50.00%), Query Frame = 1 

Query: 12 VSSTLILLLILALALILVIFCCSNDGTRKNN-----------RRGP-------WVDQFVS 66
          + ST ++L++LA  ++ V    +N G+ K N           R G        WV+  +S
Sbjct: 26 IMSTFVILILLAFGILSVP--SNNAGSSKANDLTSIVRKTLQRSGEDDSKNERWVE-IIS 85

[Close]
BLAST of Cucsa.132950 vs. TAIR10
Match: AT4G35810.1 (AT4G35810.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 105.9 bits (263), Expect = 2.3e-23
Identity = 47/84 (55.95%), Postives = 61/84 (72.62%), Query Frame = 1 

Query: 71  NIEEGGETVFPAANKCVSSVPWWKKLPTHGKDGLSIKPKMGDALFFWSMKPDGTLDYTSL 130
           +++EGGETVFPAA   VS VPWW +L   GK+GLS+ PK  DAL FWSMKPD +LD +SL
Sbjct: 207 DVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLSVLPKKRDALLFWSMKPDASLDPSSL 266

Query: 131 HGSYPVIRGDEWCGSRILFPRKYN 155
           HG  PVI+G++W  ++     +YN
Sbjct: 267 HGGCPVIKGNKWSSTKWFHVHEYN 290


HSP 2 Score: 33.1 bits (74), Expect = 1.9e-01
Identity = 13/31 (41.94%), Postives = 20/31 (64.52%), Query Frame = 1 

Query: 37 GTRKNNRRGPWVDQFVSWEPRAFICHNFLAS 68
          G  ++     W++  +SWEPRAF+ HNFL +
Sbjct: 69 GDEEDGNGDRWLE-VISWEPRAFVYHNFLTN 98

[Close]
BLAST of Cucsa.132950 vs. TAIR10
Match: AT2G17720.1 (AT2G17720.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 99.8 bits (247), Expect = 1.6e-21
Identity = 40/85 (47.06%), Postives = 62/85 (72.94%), Query Frame = 1 

Query: 71  NIEEGGETVFPAANKCVSSVPWWKKLPTHGKDGLSIKPKMGDALFFWSMKPDGTLDYTSL 130
           ++++GGETVFPAA   +S+VPWW +L   GK+GLS+ PK  DAL FW+M+PD +LD +SL
Sbjct: 207 DVDDGGETVFPAARGNISAVPWWNELSKCGKEGLSVLPKKRDALLFWNMRPDASLDPSSL 266

Query: 131 HGSYPVIRGDEWCGSRILFPRKYNI 156
           HG  PV++G++W  ++     ++ +
Sbjct: 267 HGGCPVVKGNKWSSTKWFHVHEFKV 291


HSP 2 Score: 30.8 bits (68), Expect = 9.3e-01
Identity = 12/21 (57.14%), Postives = 16/21 (76.19%), Query Frame = 1 

Query: 47 WVDQFVSWEPRAFICHNFLAS 68
          WV+  +SWEPRA + HNFL +
Sbjct: 79 WVE-VISWEPRAVVYHNFLTN 98

[Close]
BLAST of Cucsa.132950 vs. TAIR10
Match: AT2G23096.1 (AT2G23096.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 73.6 bits (179), Expect = 1.2e-13
Identity = 37/95 (38.95%), Postives = 56/95 (58.95%), Query Frame = 1 

Query: 71  NIEEGGETVFPAAN-----------KCVSSVPWWKKLPTHGKDGLSIKPKMGDALFFWSM 130
           ++EEGGET+FP  N           KCV               GL +KP+ GDA+FF+++
Sbjct: 195 SVEEGGETMFPFENGRNMNGRYDYEKCV---------------GLKVKPRQGDAIFFYNL 254

Query: 131 KPDGTLDYTSLHGSYPVIRGDEWCGSRILFPRKYN 155
            P+GT+D TSLHGS PVI+G++W  ++ +  + Y+
Sbjct: 255 FPNGTIDQTSLHGSCPVIKGEKWVATKWIRDQTYD 274

[Close]
BLAST of Cucsa.132950 vs. NCBI nr
Match: gi|976917476|gb|KVI02929.1| (Prolyl 4-hydroxylase, alpha subunit [Cynara cardunculus var. scolymus])

HSP 1 Score: 135.6 bits (340), Expect = 7.6e-29
Identity = 62/115 (53.91%), Postives = 85/115 (73.91%), Query Frame = 1 

Query: 41  NNRRGPWVDQFVSWEPRAFICHNFLASRILNIEEGGETVFPAANKCVSSVPWWKKLPTHG 100
           ++R   WV+  +SWEPRA + HNFL S + ++EEGGETVFP+A   +S+V WW +L   G
Sbjct: 70  DDRADQWVE-VISWEPRAVVYHNFLVS-MSDVEEGGETVFPSAKGNISAVSWWNQLSECG 129

Query: 101 KDGLSIKPKMGDALFFWSMKPDGTLDYTSLHGSYPVIRGDEWCGSRILFPRKYNI 156
           KDGLS+KPKMGDAL FWSMKPD +LD +SLHG  PVI+G++W  ++ +   +Y +
Sbjct: 130 KDGLSVKPKMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWIHVDEYKV 182

[Close]
BLAST of Cucsa.132950 vs. NCBI nr
Match: gi|357517881|ref|XP_003629229.1| (prolyl 4-hydroxylase alpha-like protein [Medicago truncatula])

HSP 1 Score: 118.2 bits (295), Expect = 1.2e-23
Identity = 53/72 (73.61%), Postives = 61/72 (84.72%), Query Frame = 1 

Query: 71  NIEEGGETVFPAANKCVSSVPWWKKLPTHGKDGLSIKPKMGDALFFWSMKPDGTLDYTSL 130
           ++EEGGETVFPAA   +SSVPWW +L   GK GLSIKPKMGDAL FWSMKPDGTLD +SL
Sbjct: 194 DVEEGGETVFPAAKGNISSVPWWNELSDCGKKGLSIKPKMGDALLFWSMKPDGTLDPSSL 253

Query: 131 HGSYPVIRGDEW 143
           HG+ PVI+GD+W
Sbjct: 254 HGACPVIKGDKW 265

[Close]
BLAST of Cucsa.132950 vs. NCBI nr
Match: gi|357517881|ref|XP_003629229.1| (prolyl 4-hydroxylase alpha-like protein [Medicago truncatula])

HSP 1 Score: 41.2 bits (95), Expect = 1.9e+00
Identity = 25/60 (41.67%), Postives = 38/60 (63.33%), Query Frame = 1 

Query: 12 VSSTLILLLILALALILV--IFCCSNDGTR-KNNRRGPWVDQFVSWEPRAFICHNFLASR 69
          V+ TL++L++L L +  +  I   S++  R ++N    WV Q VSWEPRAF+ HNFL  +
Sbjct: 28 VTFTLLILILLTLRIPKLNHINSISHNALRSEDNDNKRWV-QIVSWEPRAFLYHNFLTKK 86


HSP 2 Score: 117.5 bits (293), Expect = 2.1e-23
Identity = 54/83 (65.06%), Postives = 64/83 (77.11%), Query Frame = 1 

Query: 71  NIEEGGETVFPAANKCVSSVPWWKKLPTHGKDGLSIKPKMGDALFFWSMKPDGTLDYTSL 130
           ++EEGGETVFPAA    SSVPWW +L   GK GLS+KPKMGDAL FWSMKPD TLD TSL
Sbjct: 201 DVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDATLDPTSL 260

Query: 131 HGSYPVIRGDEWCGSRILFPRKY 154
           HG+ PVIRG++W  ++ +   KY
Sbjct: 261 HGACPVIRGNKWSCTKWMHVNKY 283

[Close]
BLAST of Cucsa.132950 vs. NCBI nr
Match: gi|659070731|ref|XP_008456388.1| (PREDICTED: prolyl 4-hydroxylase subunit alpha-1-like isoform X2 [Cucumis melo])

HSP 1 Score: 37.7 bits (86), Expect = 2.1e+01
Identity = 25/73 (34.25%), Postives = 35/73 (47.95%), Query Frame = 1 

Query: 14 STLILLLILALALILVIF--------------------CCSNDGTRKNNRRGPWVDQFVS 67
          S +I+ L+LAL   ++I                        +DG  K  R   WV +F+S
Sbjct: 22 SKMIMALVLALGFFMLIALRFFSPPETSHHRLPSVRRTAFQSDGLGK--RGDQWV-EFIS 81


HSP 2 Score: 117.5 bits (293), Expect = 2.1e-23
Identity = 55/83 (66.27%), Postives = 64/83 (77.11%), Query Frame = 1 

Query: 71  NIEEGGETVFPAANKCVSSVPWWKKLPTHGKDGLSIKPKMGDALFFWSMKPDGTLDYTSL 130
           ++EEGGETVFPAA    SSVPWW +L   GK GLSIKPKMGDAL FWSMKPD TLD TSL
Sbjct: 201 DVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKMGDALLFWSMKPDTTLDPTSL 260

Query: 131 HGSYPVIRGDEWCGSRILFPRKY 154
           HG+ PVIRG++W  ++ +   KY
Sbjct: 261 HGACPVIRGNKWSCTKWIHVNKY 283

[Close]
BLAST of Cucsa.132950 vs. NCBI nr
Match: gi|659070723|ref|XP_008456352.1| (PREDICTED: prolyl 4-hydroxylase subunit alpha-1-like [Cucumis melo])

HSP 1 Score: 37.4 bits (85), Expect = 2.8e+01
Identity = 18/32 (56.25%), Postives = 23/32 (71.88%), Query Frame = 1 

Query: 35 NDGTRKNNRRGPWVDQFVSWEPRAFICHNFLA 67
          +DG  K  R   WV +F+SWEPRAF+ HNFL+
Sbjct: 63 SDGLGK--RGDQWV-EFISWEPRAFVYHNFLS 91


HSP 2 Score: 117.1 bits (292), Expect = 2.8e-23
Identity = 54/86 (62.79%), Postives = 66/86 (76.74%), Query Frame = 1 

Query: 65  LASRILNIEEGGETVFPAANKCVSSV-PWWKKLPTHGKD-GLSIKPKMGDALFFWSMKPD 124
           L   +  ++EGG TVFP A KC SS   WWKKLP +GKD GLS+KPKMGDAL FWS+KPD
Sbjct: 120 LVMYLSGVKEGGATVFPEAKKCASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPD 179

Query: 125 GTLDYTSLHGSYPVIRGDEWCGSRIL 149
           GTLD TSLH S PV++GD+W G +++
Sbjct: 180 GTLDPTSLHASSPVVKGDKWVGVKLM 205

The following BLAST results are available for this feature:
BLAST of Cucsa.132950 vs. Swiss-Prot
Match NameE-valueIdentityDescription
P4H3_ARATH1.1e-2461.18Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana GN=P4H3 PE=2 SV=1[more]
P4H10_ARATH6.2e-2357.65Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana GN=P4H10 PE=2 SV=1[more]
P4H8_ARATH4.0e-2255.95Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana GN=P4H8 PE=3 SV=1[more]
P4H5_ARATH2.9e-2047.06Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana GN=P4H5 PE=2 SV=1[more]
P4H13_ARATH2.2e-1238.95Prolyl 4-hydroxylase 13 OS=Arabidopsis thaliana GN=P4H13 PE=2 SV=1[more]
BLAST of Cucsa.132950 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A103Y5K3_CYNCS5.3e-2953.91Prolyl 4-hydroxylase, alpha subunit OS=Cynara cardunculus var. scolymus GN=Ccrd_... [more]
G7LB32_MEDTR8.7e-2473.61Prolyl 4-hydroxylase alpha-like protein OS=Medicago truncatula GN=MTR_8g074810 P... [more]
G7LB32_MEDTR1.4e+0041.67Prolyl 4-hydroxylase alpha-like protein OS=Medicago truncatula GN=MTR_8g074810 P... [more]
A0A0A0LFF5_CUCSA2.5e-2365.06Uncharacterized protein OS=Cucumis sativus GN=Csa_2G009620 PE=4 SV=1[more]
A0A0A0LFF5_CUCSA2.0e+0156.25Uncharacterized protein OS=Cucumis sativus GN=Csa_2G009620 PE=4 SV=1[more]
BLAST of Cucsa.132950 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G20270.16.4e-2661.18 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT5G66060.13.5e-2457.65 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT4G35810.12.3e-2355.95 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT2G17720.11.6e-2147.06 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT2G23096.11.2e-1338.95 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
BLAST of Cucsa.132950 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|976917476|gb|KVI02929.1|7.6e-2953.91Prolyl 4-hydroxylase, alpha subunit [Cynara cardunculus var. scolymus][more]
gi|357517881|ref|XP_003629229.1|1.2e-2373.61prolyl 4-hydroxylase alpha-like protein [Medicago truncatula][more]
gi|357517881|ref|XP_003629229.1|1.9e+0041.67prolyl 4-hydroxylase alpha-like protein [Medicago truncatula][more]
gi|659070731|ref|XP_008456388.1|2.1e+0134.25PREDICTED: prolyl 4-hydroxylase subunit alpha-1-like isoform X2 [Cucumis melo][more]
gi|659070723|ref|XP_008456352.1|2.8e+0156.25PREDICTED: prolyl 4-hydroxylase subunit alpha-1-like [Cucumis melo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005794 Golgi apparatus
molecular_function GO:0005506 iron ion binding
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.132950.1Cucsa.132950.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR10869PROLYL 4-HYDROXYLASE ALPHA SUBUNITcoord: 100..142
score: 1.8E-29coord: 71..84
score: 1.8
NoneNo IPR availablePANTHERPTHR10869:SF782-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEINcoord: 100..142
score: 1.8E-29coord: 71..84
score: 1.8