Cucsa.116640 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.116640
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
Descriptioncytochrome B5-like protein
Locationscaffold00995 : 533020 .. 535442 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAGAGTGATTCTGGTAAGCATTTCCCCGGCTAATTCATTTTGGCATTCAGTTCACCGGTCAGTGGATAATCTCTCTGATTCATCTCTAAATCGGATCGGTATTCTTACGGTAAGTTCTCAGTTTCGCTCTAAATCAGCTAAATCATCAGCTTCAAAGACTAGCATGAATCAGTGACCTTGGATGGTCCGAGATTCTTTGCTGAATTTCCGAGCTCAGGAGCATTTCTCTTGCTCTTACCAAACAGATTTTTCAATTTTTTTTTtAAGATTTCAATATCTGAATCATGCTGCTTCTTGCTTTGTTTCAGTGTGCTGATTTTGCATTTATTGTGAATCTGATTTTGCAGTCAGGTACATAAAATGGTGATCGTGATAGCTGCATTGCTTCTCAGTGTTTTGTTGGGAGCTCTATTTTTAATCCCACGCGACCGTAAGTTTGAAGAAGTGTATTTGTTTCATTTCTTCAATTCTCCAATGCATAAATTCACATCGATTTGAACAGACAATGCTATTTTCTAATGCAGGAAATGCACAAAAGGTCCAACTAAATCCTATCAACAAGGTACTTTGGATATGGAGGTTTTCTTTTTACTACATTTACTGGAAATTTGAAGACTTAGTTGTCTTTTAGATTCAATTTTATAAATGAATTGATCAATTCTTTGCCAGCTGAGAAGCTGGGACGTGAATGTTTTTAGCTTCAGTAGCATAGGTAAATATTTTGGCTTGTGCAATCTTCTATTTCCAGAATCCAGCTAAAGATAATGTATTGCTTTAATATTGAATCCTGTTAAAAATATGTTGGAGTTTTCAAATGTATAATGTTCAATCTATTCAGCCTTTTCTCAAACATGAACAAGTGGGAAAAAAAGTTGTAGAGTAAATTGATTGATTTGCTAGTCCAAGTGTTTTAAATGATTGTGCTGTTGTACTAACCAACTGCTTATAACTGGAGTGCTAATGCTTTGTGGCTTGAGTCTTTGACTAGGATTATAAATACTGAACTTGTACAATGCACAGTTGAGAAACAGAAAAGAATCTGTAGTTTACAAGAGATTTAGTGAAACAGTTTGTGCCTTCATTCACCATAGAAACAAAGTTGTACGTCCAAATTTAATTCTCAAATGTTTGAAGCGCCTTTTAAGTTTTAACTCTCTGTGGGATTCCTTTCCATTGTTGGTTGGATTGCTGAACTTTAGTATTGTGATTTCAAATCTCCTGGTTTAGCATAAGCTTATTTGTTGGTAATGTGCAGTTCCGGGATAGGCCTTAGTTTTtAAAATTTAAGGATCTGCGTTGATCTTATTTTTCATTAAATTTTAAATGTTGTTTCTTGCTTTTAGTCATCGAAAATCTACTACAGTAAAGATGAAGTTTCGGTTCATAACAAGAGAACTGATTGTTGGGTCATTATCAAGAACAGGGTATAATCTTCTATCTTCTGGTTTGATTTCCAAGCTCTTAGCAAAAGTTAGAGTTTGCTTGTATATTATTGTTGTACTTATAATCTTTTTTCGATTATACTTGAGCATAGGTGTATGATGTTACCTCCTATGTTGAAGAACATCCTGGAGGGGATGCCATCCTAACACATGCTGGGGATGATTCTACAGAAGGGTTTTATGGGTAAACCAAGCGGACATCACCTCATCTGTATTATGTCATGTTAAATTGATATGTCCAAACAATCTTGATTGAATTGCTCTAAAATTGTTTTGGCATAAATAGACAATAATTGAGATCACCATGCTTGATTCACTAAACAAAGACCATCGAGTGAGAGAATATTGCCTATTAACTTTCACACGCAACTAGACAGCATTCACTAAGAAAGATACCGAACCTTTTCCCTTCATTTTTACTTCAAACTATAAGGAACATGTTTGGTTTTACTCAACATACAAATAAGCTATAATATTAGATTCCATCAACGCAACAATCTTCTAAACATGTTAATTTCAACCTCGATACTTATTATGCAGGCCACAACACGCAACTCGAGTCTTTGACATGATTGAAGACTTCTATATTGGAGATCTGAAGCTGTAACATCCAAATAATGTTTGGCTTTGTGCTGTTAATTCGAGGACGTATTTCACATCGCAGATCTGGATTTGCCATTGCAGGGTATGTATGTAAAGATCTGAATTATCTAGTTAGCATTCTTGAAAAATTGTAACATCTATAGATGGTGTAGACTTAATTTAAATCTTCTCCTTTTTAGATTTATATCTTTACATTTCTTGTGGATATTCTGATTTCTAATTTGCTAGAGGGGAGAGACTTTTCATTGTAGTCTTGTTTAATTTGTATTTTCTGTTGTAGCATTTGGTAAAGTGCTTTTTTATTCTTCAAGAATTGATCTGGTTTGTGTAATCTGATTTGGTGCTGTTGAGATTCTTGAGACCCATCTGACTGTAGA

mRNA sequence

TGAGAGTGATTCTGGTAAGCATTTCCCCGGCTAATTCATTTTGGCATTCAGTTCACCGGTCAGTGGATAATCTCTCTGATTCATCTCTAAATCGGATCGGTATTCTTACGTCAGGTACATAAAATGGTGATCGTGATAGCTGCATTGCTTCTCAGTGTTTTGTTGGGAGCTCTATTTTTAATCCCACGCGACCGAAATGCACAAAAGGTCCAACTAAATCCTATCAACAAGTCATCGAAAATCTACTACAGTAAAGATGAAGTTTCGGTTCATAACAAGAGAACTGATTGTTGGGTCATTATCAAGAACAGGGTGTATGATGTTACCTCCTATGTTGAAGAACATCCTGGAGGGGATGCCATCCTAACACATGCTGGGGATGATTCTACAGAAGGGTTTTATGGGCCACAACACGCAACTCGAGTCTTTGACATGATTGAAGACTTCTATATTGGAGATCTGAAGCTGTAACATCCAAATAATGTTTGGCTTTGTGCTGTTAATTCGAGGACGTATTTCACATCGCAGATCTGGATTTGCCATTGCAGGGTATGTATGTAAAGATCTGAATTATCTAGTTAGCATTCTTGAAAAATTGTAACATCTATAGATGGTGTAGACTTAATTTAAATCTTCTCCTTTTTAGATTTATATCTTTACATTTCTTGTGGATATTCTGATTTCTAATTTGCTAGAGGGGAGAGACTTTTCATTGTAGTCTTGTTTAATTTGTATTTTCTGTTGTAGCATTTGGTAAAGTGCTTTTTTATTCTTCAAGAATTGATCTGGTTTGTGTAATCTGATTTGGTGCTGTTGAGATTCTTGAGACCCATCTGACTGTAGA

Coding sequence (CDS)

ATGGTGATCGTGATAGCTGCATTGCTTCTCAGTGTTTTGTTGGGAGCTCTATTTTTAATCCCACGCGACCGAAATGCACAAAAGGTCCAACTAAATCCTATCAACAAGTCATCGAAAATCTACTACAGTAAAGATGAAGTTTCGGTTCATAACAAGAGAACTGATTGTTGGGTCATTATCAAGAACAGGGTGTATGATGTTACCTCCTATGTTGAAGAACATCCTGGAGGGGATGCCATCCTAACACATGCTGGGGATGATTCTACAGAAGGGTTTTATGGGCCACAACACGCAACTCGAGTCTTTGACATGATTGAAGACTTCTATATTGGAGATCTGAAGCTGTAA

Protein sequence

MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKIYYSKDEVSVHNKRTDCWVIIKNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL*
Homology
[Close]
BLAST of Cucsa.116640 vs. Swiss-Prot
Match: CYP5F_ARATH (Cytochrome B5-like protein OS=Arabidopsis thaliana GN=CB5LP PE=2 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 3.1e-35
Identity = 74/120 (61.67%), Postives = 86/120 (71.67%), Query Frame = 1 

Query: 2   VIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKIY--------YSKDEVSVHNKR 61
           +I +  LLL  L+ ALFLI   R            SS+          YSK EV+VHNKR
Sbjct: 1   MIAVIGLLLGFLVSALFLIQGKRRRTNDNQEKKRSSSEPVEDVVRPKSYSKSEVAVHNKR 60

Query: 62  TDCWVIIKNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDL 114
            DCW+IIK++VYD+TSYVEEHPGGDAIL HAGDDST+GF+GPQHATRVFDMIEDFYIG+L
Sbjct: 61  NDCWIIIKDKVYDITSYVEEHPGGDAILDHAGDDSTDGFFGPQHATRVFDMIEDFYIGEL 120

[Close]
BLAST of Cucsa.116640 vs. Swiss-Prot
Match: DES6_PHYPA (Acyl-lipid (9-3)-desaturase OS=Physcomitrella patens subsp. patens GN=DES6 PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.1e-15
Identity = 34/69 (49.28%), Postives = 52/69 (75.36%), Query Frame = 1 

Query: 46  EVSVHNKRTDCWVIIKNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMI 105
           EV+VHNK +DCW+++KN+VYDV+++ +EHPGG  I T+ G D T+ F    HA   + ++
Sbjct: 109 EVAVHNKPSDCWIVVKNKVYDVSNFADEHPGGSVISTYFGRDGTDVF-SSFHAASTWKIL 168

Query: 106 EDFYIGDLK 115
           +DFYIGD++
Sbjct: 169 QDFYIGDVE 176

[Close]
BLAST of Cucsa.116640 vs. Swiss-Prot
Match: CYB5A_ARATH (Cytochrome b5 isoform A OS=Arabidopsis thaliana GN=CB5-A PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 1.8e-14
Identity = 34/73 (46.58%), Postives = 52/73 (71.23%), Query Frame = 1 

Query: 42  YSKDEVSVHNKRTDCWVIIKNRVYDVTSYVEEHPGG-DAILTHAGDDSTEGFYGPQHATR 101
           YS +E + HNK+ DCWV+I  +VYDV+SY++EHPGG D +L  AG D+T+ F    H+  
Sbjct: 8   YSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHSKD 67

Query: 102 VFDMIEDFYIGDL 114
             +++E ++IG+L
Sbjct: 68  ARELMEKYFIGEL 80

[Close]
BLAST of Cucsa.116640 vs. Swiss-Prot
Match: CYB5_ORYSJ (Cytochrome b5 OS=Oryza sativa subsp. japonica GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 76.6 bits (187), Expect = 1.9e-13
Identity = 31/81 (38.27%), Postives = 56/81 (69.14%), Query Frame = 1 

Query: 34  INKSSKIYYSKDEVSVHNKRTDCWVIIKNRVYDVTSYVEEHPGG-DAILTHAGDDSTEGF 93
           ++  +K  Y+ +EV+ HN + DCW+II  +VY+V+ ++E+HPGG D +L+  G D+T+ F
Sbjct: 1   MSNDNKKVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDF 60

Query: 94  YGPQHATRVFDMIEDFYIGDL 114
               H+T    M++++Y+GD+
Sbjct: 61  EDVGHSTTARAMMDEYYVGDI 81

[Close]
BLAST of Cucsa.116640 vs. Swiss-Prot
Match: CYB5_BRAOB (Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.9e-13
Identity = 35/78 (44.87%), Postives = 50/78 (64.10%), Query Frame = 1 

Query: 37  SSKIYYSKDEVSVHNKRTDCWVIIKNRVYDVTSYVEEHPGGD-AILTHAGDDSTEGFYGP 96
           S K     +EVS HNK  DCW+II  +VYDVT ++++HPGGD  +L+  G D+T  F   
Sbjct: 3   SEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDV 62

Query: 97  QHATRVFDMIEDFYIGDL 114
            H+    DM+E +YIG++
Sbjct: 63  GHSDTARDMMEKYYIGEI 80

[Close]
BLAST of Cucsa.116640 vs. TrEMBL
Match: A0A0A0LFU2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G000900 PE=3 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 8.1e-59
Identity = 114/115 (99.13%), Postives = 115/115 (100.00%), Query Frame = 1 

Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKIYYSKDEVSVHNKRTDCWVII 60
           MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSK+YYSKDEVSVHNKRTDCWVII
Sbjct: 121 MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSKDEVSVHNKRTDCWVII 180

Query: 61  KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 116
           KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL
Sbjct: 181 KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 235

[Close]
BLAST of Cucsa.116640 vs. TrEMBL
Match: A0A067FGQ3_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g036360mg PE=3 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 3.2e-39
Identity = 83/118 (70.34%), Postives = 95/118 (80.51%), Query Frame = 1 

Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPI----NKSSKIYYSKDEVSVHNKRTDC 60
           MVI++AAL+L  +LG    IPR   +   +        NK+SKIY SKDEV++HNKR DC
Sbjct: 1   MVIIVAALILGAILGVFIFIPRHPTSGHSKSTHSSANSNKASKIY-SKDEVTLHNKRDDC 60

Query: 61  WVIIKNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLK 115
           W+IIKN+VYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHATRVFDMI+DFYIGDLK
Sbjct: 61  WIIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYIGDLK 117

[Close]
BLAST of Cucsa.116640 vs. TrEMBL
Match: V4UPZ7_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10009944mg PE=3 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 4.2e-39
Identity = 83/118 (70.34%), Postives = 94/118 (79.66%), Query Frame = 1 

Query: 1   MVIVIAALLLSVLLGALFLIPRD----RNAQKVQLNPINKSSKIYYSKDEVSVHNKRTDC 60
           MVI++AAL+L  +LG    IPR      +         NK+SKIY SKDEV++HNKR DC
Sbjct: 1   MVIIVAALILGAILGVFIFIPRHPASGHSKSTHSSANSNKASKIY-SKDEVTLHNKRDDC 60

Query: 61  WVIIKNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLK 115
           W+IIKN+VYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHATRVFDMI+DFYIGDLK
Sbjct: 61  WIIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYIGDLK 117

[Close]
BLAST of Cucsa.116640 vs. TrEMBL
Match: A0A0D2UIB7_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_010G244200 PE=3 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 5.5e-39
Identity = 82/115 (71.30%), Postives = 95/115 (82.61%), Query Frame = 1 

Query: 1   MVIVIAALLLSVLLGALFLIPRD-RNAQKVQLNPINKSSKIYYSKDEVSVHNKRTDCWVI 60
           M I I AL+L+VLLGA  L+PR  + A K ++     +SK  YSK EV +HNKRTDCW+I
Sbjct: 1   MEIAIIALILAVLLGAFILVPRHGKLAHKNKVKSTVANSKASYSKTEVMLHNKRTDCWII 60

Query: 61  IKNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLK 115
           IK++VYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHATRVFDMI+DFYIGDL+
Sbjct: 61  IKDKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYIGDLQ 115

[Close]
BLAST of Cucsa.116640 vs. TrEMBL
Match: A0A061GU94_THECC (Cytochrome B5-like protein, n5,ATCB5LP,CB5LP OS=Theobroma cacao GN=TCM_037790 PE=3 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 7.1e-39
Identity = 81/117 (69.23%), Postives = 93/117 (79.49%), Query Frame = 1 

Query: 1   MVIVIAALLLSVLLGALFLIPR---DRNAQKVQLNPINKSSKIYYSKDEVSVHNKRTDCW 60
           M I + AL+L VLLGA  LIPR     +  +VQ N  N  +   YSK EV++HNKRTDCW
Sbjct: 1   MAIAVIALILGVLLGAFILIPRHGKSAHTNQVQSNGNNSKASKSYSKTEVALHNKRTDCW 60

Query: 61  VIIKNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLK 115
           +IIK++VYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHAT VFDMI+DFYIGDL+
Sbjct: 61  IIIKDKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATSVFDMIDDFYIGDLQ 117

[Close]
BLAST of Cucsa.116640 vs. TAIR10
Match: AT1G60660.1 (AT1G60660.1 cytochrome B5-like protein)

HSP 1 Score: 149.1 bits (375), Expect = 1.7e-36
Identity = 74/120 (61.67%), Postives = 86/120 (71.67%), Query Frame = 1 

Query: 2   VIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKIY--------YSKDEVSVHNKR 61
           +I +  LLL  L+ ALFLI   R            SS+          YSK EV+VHNKR
Sbjct: 1   MIAVIGLLLGFLVSALFLIQGKRRRTNDNQEKKRSSSEPVEDVVRPKSYSKSEVAVHNKR 60

Query: 62  TDCWVIIKNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDL 114
            DCW+IIK++VYD+TSYVEEHPGGDAIL HAGDDST+GF+GPQHATRVFDMIEDFYIG+L
Sbjct: 61  NDCWIIIKDKVYDITSYVEEHPGGDAILDHAGDDSTDGFFGPQHATRVFDMIEDFYIGEL 120

[Close]
BLAST of Cucsa.116640 vs. TAIR10
Match: AT1G26340.1 (AT1G26340.1 cytochrome B5 isoform A)

HSP 1 Score: 80.1 bits (196), Expect = 9.9e-16
Identity = 34/73 (46.58%), Postives = 52/73 (71.23%), Query Frame = 1 

Query: 42  YSKDEVSVHNKRTDCWVIIKNRVYDVTSYVEEHPGG-DAILTHAGDDSTEGFYGPQHATR 101
           YS +E + HNK+ DCWV+I  +VYDV+SY++EHPGG D +L  AG D+T+ F    H+  
Sbjct: 8   YSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHSKD 67

Query: 102 VFDMIEDFYIGDL 114
             +++E ++IG+L
Sbjct: 68  ARELMEKYFIGEL 80

[Close]
BLAST of Cucsa.116640 vs. TAIR10
Match: AT5G48810.1 (AT5G48810.1 cytochrome B5 isoform D)

HSP 1 Score: 75.1 bits (183), Expect = 3.2e-14
Identity = 30/73 (41.10%), Postives = 50/73 (68.49%), Query Frame = 1 

Query: 42  YSKDEVSVHNKRTDCWVIIKNRVYDVTSYVEEHPGGD-AILTHAGDDSTEGFYGPQHATR 101
           ++  EVS H+   DCW++I  +VYDVT ++++HPGGD  ILT  G D+T+ F    H++ 
Sbjct: 8   FTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHSST 67

Query: 102 VFDMIEDFYIGDL 114
              M++++Y+GD+
Sbjct: 68  AKAMLDEYYVGDI 80

[Close]
BLAST of Cucsa.116640 vs. TAIR10
Match: AT5G53560.1 (AT5G53560.1 cytochrome B5 isoform E)

HSP 1 Score: 74.3 bits (181), Expect = 5.4e-14
Identity = 33/78 (42.31%), Postives = 51/78 (65.38%), Query Frame = 1 

Query: 37  SSKIYYSKDEVSVHNKRTDCWVIIKNRVYDVTSYVEEHPGGD-AILTHAGDDSTEGFYGP 96
           S +   S +EVS HNK  DCW+II  +VYDVT ++++HPGGD  +L+  G D+T  F   
Sbjct: 3   SDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDV 62

Query: 97  QHATRVFDMIEDFYIGDL 114
            H+    DM++ ++IG++
Sbjct: 63  GHSDTARDMMDKYFIGEI 80

[Close]
BLAST of Cucsa.116640 vs. TAIR10
Match: AT2G32720.1 (AT2G32720.1 cytochrome B5 isoform B)

HSP 1 Score: 73.6 bits (179), Expect = 9.3e-14
Identity = 29/73 (39.73%), Postives = 50/73 (68.49%), Query Frame = 1 

Query: 42  YSKDEVSVHNKRTDCWVIIKNRVYDVTSYVEEHPGG-DAILTHAGDDSTEGFYGPQHATR 101
           ++  EVS HN+  DCW++I  +VY+VT ++E+HPGG D +L+  G D+T+ F    H+  
Sbjct: 8   FTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSES 67

Query: 102 VFDMIEDFYIGDL 114
             +M+E +Y+G++
Sbjct: 68  AREMMEQYYVGEI 80

[Close]
BLAST of Cucsa.116640 vs. NCBI nr
Match: gi|449446520|ref|XP_004141019.1| (PREDICTED: cytochrome B5-like protein [Cucumis sativus])

HSP 1 Score: 234.2 bits (596), Expect = 1.2e-58
Identity = 114/115 (99.13%), Postives = 115/115 (100.00%), Query Frame = 1 

Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKIYYSKDEVSVHNKRTDCWVII 60
           MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSK+YYSKDEVSVHNKRTDCWVII
Sbjct: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSKDEVSVHNKRTDCWVII 60

Query: 61  KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 116
           KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL
Sbjct: 61  KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 115

[Close]
BLAST of Cucsa.116640 vs. NCBI nr
Match: gi|700205464|gb|KGN60583.1| (hypothetical protein Csa_2G000900 [Cucumis sativus])

HSP 1 Score: 234.2 bits (596), Expect = 1.2e-58
Identity = 114/115 (99.13%), Postives = 115/115 (100.00%), Query Frame = 1 

Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKIYYSKDEVSVHNKRTDCWVII 60
           MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSK+YYSKDEVSVHNKRTDCWVII
Sbjct: 121 MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSKDEVSVHNKRTDCWVII 180

Query: 61  KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 116
           KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL
Sbjct: 181 KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 235

[Close]
BLAST of Cucsa.116640 vs. NCBI nr
Match: gi|659071233|ref|XP_008458811.1| (PREDICTED: cytochrome b5 [Cucumis melo])

HSP 1 Score: 233.0 bits (593), Expect = 2.6e-58
Identity = 113/115 (98.26%), Postives = 115/115 (100.00%), Query Frame = 1 

Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKIYYSKDEVSVHNKRTDCWVII 60
           MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSK+YYSKDEVSVHNKRTDCWVII
Sbjct: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSKDEVSVHNKRTDCWVII 60

Query: 61  KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 116
           KN+VYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL
Sbjct: 61  KNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 115

[Close]
BLAST of Cucsa.116640 vs. NCBI nr
Match: gi|568843333|ref|XP_006475567.1| (PREDICTED: cytochrome B5-like protein [Citrus sinensis])

HSP 1 Score: 169.5 bits (428), Expect = 3.5e-39
Identity = 84/119 (70.59%), Postives = 96/119 (80.67%), Query Frame = 1 

Query: 1   MVIVIAALLLSVLLGALFLIPRD-----RNAQKVQLNPINKSSKIYYSKDEVSVHNKRTD 60
           MVI++AAL+L  +LG    IPR        + +   N  NK+SKIY SKDEV++HNKR D
Sbjct: 1   MVIIVAALILGAILGVFIFIPRHPASGHSKSTRSSANS-NKASKIY-SKDEVTLHNKRDD 60

Query: 61  CWVIIKNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLK 115
           CW+IIKN+VYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHATRVFDMI+DFYIGDLK
Sbjct: 61  CWIIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYIGDLK 117

[Close]
BLAST of Cucsa.116640 vs. NCBI nr
Match: gi|641847695|gb|KDO66574.1| (hypothetical protein CISIN_1g036360mg [Citrus sinensis])

HSP 1 Score: 169.1 bits (427), Expect = 4.6e-39
Identity = 83/118 (70.34%), Postives = 95/118 (80.51%), Query Frame = 1 

Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPI----NKSSKIYYSKDEVSVHNKRTDC 60
           MVI++AAL+L  +LG    IPR   +   +        NK+SKIY SKDEV++HNKR DC
Sbjct: 1   MVIIVAALILGAILGVFIFIPRHPTSGHSKSTHSSANSNKASKIY-SKDEVTLHNKRDDC 60

Query: 61  WVIIKNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLK 115
           W+IIKN+VYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHATRVFDMI+DFYIGDLK
Sbjct: 61  WIIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYIGDLK 117

The following BLAST results are available for this feature:
BLAST of Cucsa.116640 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CYP5F_ARATH3.1e-3561.67Cytochrome B5-like protein OS=Arabidopsis thaliana GN=CB5LP PE=2 SV=1[more]
DES6_PHYPA2.1e-1549.28Acyl-lipid (9-3)-desaturase OS=Physcomitrella patens subsp. patens GN=DES6 PE=1 ... [more]
CYB5A_ARATH1.8e-1446.58Cytochrome b5 isoform A OS=Arabidopsis thaliana GN=CB5-A PE=1 SV=1[more]
CYB5_ORYSJ1.9e-1338.27Cytochrome b5 OS=Oryza sativa subsp. japonica GN=Os05g0108800 PE=2 SV=2[more]
CYB5_BRAOB1.9e-1344.87Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1[more]
BLAST of Cucsa.116640 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LFU2_CUCSA8.1e-5999.13Uncharacterized protein OS=Cucumis sativus GN=Csa_2G000900 PE=3 SV=1[more]
A0A067FGQ3_CITSI3.2e-3970.34Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g036360mg PE=3 SV=1[more]
V4UPZ7_9ROSI4.2e-3970.34Uncharacterized protein OS=Citrus clementina GN=CICLE_v10009944mg PE=3 SV=1[more]
A0A0D2UIB7_GOSRA5.5e-3971.30Uncharacterized protein OS=Gossypium raimondii GN=B456_010G244200 PE=3 SV=1[more]
A0A061GU94_THECC7.1e-3969.23Cytochrome B5-like protein, n5,ATCB5LP,CB5LP OS=Theobroma cacao GN=TCM_037790 PE... [more]
BLAST of Cucsa.116640 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G60660.11.7e-3661.67 cytochrome B5-like protein[more]
AT1G26340.19.9e-1646.58 cytochrome B5 isoform A[more]
AT5G48810.13.2e-1441.10 cytochrome B5 isoform D[more]
AT5G53560.15.4e-1442.31 cytochrome B5 isoform E[more]
AT2G32720.19.3e-1439.73 cytochrome B5 isoform B[more]
BLAST of Cucsa.116640 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449446520|ref|XP_004141019.1|1.2e-5899.13PREDICTED: cytochrome B5-like protein [Cucumis sativus][more]
gi|700205464|gb|KGN60583.1|1.2e-5899.13hypothetical protein Csa_2G000900 [Cucumis sativus][more]
gi|659071233|ref|XP_008458811.1|2.6e-5898.26PREDICTED: cytochrome b5 [Cucumis melo][more]
gi|568843333|ref|XP_006475567.1|3.5e-3970.59PREDICTED: cytochrome B5-like protein [Citrus sinensis][more]
gi|641847695|gb|KDO66574.1|4.6e-3970.34hypothetical protein CISIN_1g036360mg [Citrus sinensis][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001199Cyt_B5-like_heme/steroid-bd
IPR018506Cyt_B5_heme-BS
Vocabulary: Molecular Function
TermDefinition
GO:0020037heme binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006040 amino sugar metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0016126 sterol biosynthetic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004128 cytochrome-b5 reductase activity, acting on NAD(P)H
molecular_function GO:0020037 heme binding
molecular_function GO:0046872 metal ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.116640.1Cucsa.116640.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001199Cytochrome b5-like heme/steroid binding domainPRINTSPR00363CYTOCHROMEB5coord: 101..113
score: 3.8E-6coord: 74..88
score: 3.8E-6coord: 64..74
score: 3.
IPR001199Cytochrome b5-like heme/steroid binding domainGENE3DG3DSA:3.10.120.10coord: 41..114
score: 2.3
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 44..113
score: 1.9
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 42..114
score: 3.6
IPR001199Cytochrome b5-like heme/steroid binding domainPROFILEPS50255CYTOCHROME_B5_2coord: 39..114
score: 24
IPR001199Cytochrome b5-like heme/steroid binding domainunknownSSF55856Cytochrome b5-like heme/steroid binding domaincoord: 38..114
score: 7.72
IPR018506Cytochrome b5, heme-binding sitePROSITEPS00191CYTOCHROME_B5_1coord: 70..77
scor
NoneNo IPR availablePANTHERPTHR19359CYTOCHROME B5coord: 1..114
score: 8.6
NoneNo IPR availablePANTHERPTHR19359:SF10SUBFAMILY NOT NAMEDcoord: 1..114
score: 8.6

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.116640Cla016796Watermelon (97103) v1cgywmB175
Cucsa.116640Csa2G000900Cucumber (Chinese Long) v2cgycuB150
Cucsa.116640MELO3C004588Melon (DHL92) v3.5.1cgymeB172
Cucsa.116640ClCG11G002140Watermelon (Charleston Gray)cgywcgB171
Cucsa.116640CSPI02G00840Wild cucumber (PI 183967)cgycpiB152
Cucsa.116640CmaCh12G012710Cucurbita maxima (Rimu)cgycmaB0285
Cucsa.116640CmoCh12G013050Cucurbita moschata (Rifu)cgycmoB0281
Cucsa.116640Lsi11G002270Bottle gourd (USVL1VR-Ls)cgylsiB163
Cucsa.116640Cp4.1LG07g10270Cucurbita pepo (Zucchini)cgycpeB0279
Cucsa.116640MELO3C004588.2Melon (DHL92) v3.6.1cgymedB170
Cucsa.116640CsaV3_2G001920Cucumber (Chinese Long) v3cgycucB156
Cucsa.116640Cla97C11G208530Watermelon (97103) v2cgywmbB167
Cucsa.116640Bhi06G001461Wax gourdcgywgoB196
Cucsa.116640Carg18596Silver-seed gourdcarcgyB1014
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.116640Silver-seed gourdcarcgyB0040
Cucsa.116640Silver-seed gourdcarcgyB0111
Cucsa.116640Silver-seed gourdcarcgyB0239
Cucsa.116640Silver-seed gourdcarcgyB0644
Cucsa.116640Watermelon (97103) v2cgywmbB168
Cucsa.116640Watermelon (97103) v2cgywmbB171
Cucsa.116640Wax gourdcgywgoB199
Cucsa.116640Wax gourdcgywgoB202
Cucsa.116640Cucumber (Gy14) v1cgycgyB067
Cucsa.116640Cucurbita maxima (Rimu)cgycmaB0283
Cucsa.116640Cucurbita maxima (Rimu)cgycmaB0284
Cucsa.116640Cucurbita maxima (Rimu)cgycmaB0288
Cucsa.116640Cucurbita moschata (Rifu)cgycmoB0279
Cucsa.116640Cucurbita moschata (Rifu)cgycmoB0280
Cucsa.116640Cucurbita moschata (Rifu)cgycmoB0285
Cucsa.116640Wild cucumber (PI 183967)cgycpiB149
Cucsa.116640Cucumber (Chinese Long) v2cgycuB148
Cucsa.116640Melon (DHL92) v3.5.1cgymeB168
Cucsa.116640Melon (DHL92) v3.5.1cgymeB171
Cucsa.116640Watermelon (Charleston Gray)cgywcgB172
Cucsa.116640Watermelon (Charleston Gray)cgywcgB175
Cucsa.116640Watermelon (97103) v1cgywmB173
Cucsa.116640Watermelon (97103) v1cgywmB174
Cucsa.116640Cucurbita pepo (Zucchini)cgycpeB0273
Cucsa.116640Cucurbita pepo (Zucchini)cgycpeB0276
Cucsa.116640Cucurbita pepo (Zucchini)cgycpeB0278
Cucsa.116640Bottle gourd (USVL1VR-Ls)cgylsiB164
Cucsa.116640Bottle gourd (USVL1VR-Ls)cgylsiB166
Cucsa.116640Melon (DHL92) v3.6.1cgymedB167