CmaCh12G012710 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh12G012710
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Cytochrome b5-like heme/steroid-binding domain protein
Location	Cma_Chr12 : 9906853 .. 9909200 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGCCCCAAGATGAAGCCCAATTGAATATGCAAACTCAGGCCCATCCCAAAGTGAAAGTCATGTGGCTCAAATTTGAGGCCCAATCTAGTAAGGAAACTGATGCCGAACCGAAAGTAGAAGTCAGTCTGGTGTTTGATGGGGATCCGGTGGGCAGAGTGCAGTGGAAATTCGAGGGTCTAGATTTTACCGGCGAATTCATTTCGGCATTCAGTTCGCCGGATATTCGATAAGCTCACTGCTTAGTCGCTAAATCCGATCCATATTCTTTAGGTAAGTTCTCAGTTTCGCATCGCTTAGCTAAATCATCTGCATCAAAACTAGCTTAAATCAGTGACCGGATGGTCCGAGAATCTTTGCTGAATTTGTGGATTCTGGAGCATTTTCTTGCTTACCAAACAAAATTTTTATTTCTTAGTTTCAATTTCGGAATGGTAATGCTTCGTTGCAGTACTATGTTTTGCATTTATTGTGAATCTGATTTAGCAGTGAGCTATATAAAATGATGATCATTGTAGCCGCATTGGTTCTCGGTGTGTTGTTTGGCGCTCTGATTTTAATCCCACGCGACCGTAAGCTTGGTGAAGTGTATTGTTTCAGTTCTTTAATCCTTTTATGCATAAATTCACATCGATTTGAACAAACAATGCTCTTATTTTCTGGATGCAGGAAATGCACAAAAGGTTCAATTAAATTCTACCAACAAGGTAAATTTGGACTTGTTTTCCTTTAGTTTCAATTTTTAAATGAATTAAGCAACCCTTTGCCCGCTTAGAACTGGAACTTAAATTGTAAATCATCATGGTTTGTGCAATTTTCTATTTCCAGATTTCAACTATGGAGAATGTATTGCTTTTCCATTGGATCCGACTAAAACTAGGATTTGTTGAGGATTGTTGGGAGAGAGTCCCACAGTAGCTAATTTAGGGAATGATCATGGGTTTATAAGTAAGGAATACATCTCTATTGGTATGAGGCTTTTTGGAGAAGCCCAAAGCAAAGCCAAGAGAACTTATGCTCTGGATAATATCATACTATTGTGGTGTGGAGAGTCGTAATTTCTAACAGGATTTAGAAATAGAAATGGAAATAGAACATTCCAAGAGATTTAGTGAAACTGAAACAATTTGTACAATTTTCTTTTTCGAGCTTATCTGTTGATGCTGCAAACTTTAGTGATGGGGCTTATTTGTGTTCAATTAAAGGATCTGCGTTGATATTTCATATAGGAAGAAGTGTTCTTTGTGGTTTCTCATTATATTCTAAAAGTTTTTCTTGCTTATAGGCATCTAAAGTGTACGGTAAAGACGAGGTCTCGGTTCATAACAAGAGAACTGATTGCTGGATCATTATCAAGAACAAGGTATTATCGGATGTCTAAGCTCGTAGCAAAACTTGGGGTTTGCTTGTATGTTGTCATAATTGTAATCTATTTTTCGATTTTTATCGAGCATAGGTGTATGATGTTACGTCTTATGTTGAAGAACATCCAGGAGGAGATGCCATCCTAGCACATGCTGGGGATGATTCTACAGAAGGGTTTTATGGGTAAGACAATCTGACACCTCCTCTCTCAATCATTGTTAGATCATATTAATTTGATCTGTCCAGATAATCTGGATTGGATACTCTTATCATTGTTTTAGCTTTGATAAACAATAATTAAGATCAGCAAAGCTTTTTCAAAATGGTTGCTTCTGCGATCCTTTTTTCCCCTGTTTTGTATGCAAACTACAGAATCATGGTGTAAAAATCGATAGCACTTGGATTTGTCAATGGTGGTATATTGATTTTAGTTTGGTTAGCATGTTCATATCACACACTCTGTCATTTGTTGAACACATCGAGCATTTGTTATGTATACTAAATAGATATATAATAATAAATTTTAAGAAATGCATAAACTTAAGGACTTTGAATTCTCTTCTTGTATAAAAATAGTAATCTGGAAAAAATATATTATTTGAATAAATGTGTCCTTGTCGTTTCTATGTCCTAAAATTTTAGATTATGACGTGCATAATGTGCGTCATGTTGTATAAGTGTCTCTTATTTGTATATGTGCTTTTTAGCATGTGGAGGTGAAGATTCGAACACATCTGACTTTGTGATCGATGATATATGTTCTAACCAATTGAGTTATGCTCAGGTTGAGCTCAAAGATCCTATATATAAAAAAGCGGTCAAATTGGCTTTACTCAAAGTTTTACCTTTCTTTCTTCCATCGTTGCAGCAATCTTCCGAACATGTTTATTTCAACCTCAATATTTTTTTGTGCAGGCCACAACATGCAACTCGAGTCTTCGACATGATCGACGACTTCTATGTTGGAGATCTGAGGCTGTAA

mRNA sequence

ATGTGGCCCCAAGATGAAGCCCAATTGAATATGCAAACTCAGGCCCATCCCAAAGTGAAAGTCATGTGGCTCAAATTTGAGGCCCAATCTAGTAAGGAAACTGATGCCGAACCGAAAGTAGAAGTCAGTCTGGTGTTTGATGGGGATCCGGTGGGCAGAGTGCACAGTGAGCTATATAAAATGATGATCATTGTAGCCGCATTGGTTCTCGGTGTGTTGTTTGGCGCTCTGATTTTAATCCCACGCGACCGAAATGCACAAAAGGTTCAATTAAATTCTACCAACAAGGCATCTAAAGTGTACGGTAAAGACGAGGTCTCGGTTCATAACAAGAGAACTGATTGCTGGATCATTATCAAGAACAAGGTGTATGATGTTACGTCTTATGTTGAAGAACATCCAGGAGGAGATGCCATCCTAGCACATGCTGGGGATGATTCTACAGAAGGGTTTTATGGGCCACAACATGCAACTCGAGTCTTCGACATGATCGACGACTTCTATGTTGGAGATCTGAGGCTGTAA

Coding sequence (CDS)

ATGTGGCCCCAAGATGAAGCCCAATTGAATATGCAAACTCAGGCCCATCCCAAAGTGAAAGTCATGTGGCTCAAATTTGAGGCCCAATCTAGTAAGGAAACTGATGCCGAACCGAAAGTAGAAGTCAGTCTGGTGTTTGATGGGGATCCGGTGGGCAGAGTGCACAGTGAGCTATATAAAATGATGATCATTGTAGCCGCATTGGTTCTCGGTGTGTTGTTTGGCGCTCTGATTTTAATCCCACGCGACCGAAATGCACAAAAGGTTCAATTAAATTCTACCAACAAGGCATCTAAAGTGTACGGTAAAGACGAGGTCTCGGTTCATAACAAGAGAACTGATTGCTGGATCATTATCAAGAACAAGGTGTATGATGTTACGTCTTATGTTGAAGAACATCCAGGAGGAGATGCCATCCTAGCACATGCTGGGGATGATTCTACAGAAGGGTTTTATGGGCCACAACATGCAACTCGAGTCTTCGACATGATCGACGACTTCTATGTTGGAGATCTGAGGCTGTAA

Protein sequence

MWPQDEAQLNMQTQAHPKVKVMWLKFEAQSSKETDAEPKVEVSLVFDGDPVGRVHSELYKMMIIVAALVLGVLFGALILIPRDRNAQKVQLNSTNKASKVYGKDEVSVHNKRTDCWIIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL

Homology

[Close]

BLAST of CmaCh12G012710 vs. Swiss-Prot
Match: CYP5F_ARATH (Cytochrome B5-like protein OS=Arabidopsis thaliana GN=CB5LP PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 1.8e-34
Identity = 75/120 (62.50%), Postives = 89/120 (74.17%), Query Frame = 1


Query: 62  MIIVAALVLGVLFGALILIP----RDRNAQKVQLNSTNKASKV-----YGKDEVSVHNKR 121
           MI V  L+LG L  AL LI     R  + Q+ + +S+     V     Y K EV+VHNKR
Sbjct: 1   MIAVIGLLLGFLVSALFLIQGKRRRTNDNQEKKRSSSEPVEDVVRPKSYSKSEVAVHNKR 60

Query: 122 TDCWIIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDL 173
            DCWIIIK+KVYD+TSYVEEHPGGDAIL HAGDDST+GF+GPQHATRVFDMI+DFY+G+L
Sbjct: 61  NDCWIIIKDKVYDITSYVEEHPGGDAILDHAGDDSTDGFFGPQHATRVFDMIEDFYIGEL 120

[Close]

BLAST of CmaCh12G012710 vs. Swiss-Prot
Match: CYB5_ORYSJ (Cytochrome b5 OS=Oryza sativa subsp. japonica GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 82.8 bits (203), Expect = 4.1e-15
Identity = 36/80 (45.00%), Postives = 55/80 (68.75%), Query Frame = 1


Query: 94  TNKASKVYGKDEVSVHNKRTDCWIIIKNKVYDVTSYVEEHPGG-DAILAHAGDDSTEGFY 153
           +N   KVY  +EV+ HN + DCW+II  KVY+V+ ++E+HPGG D +L+  G D+T+ F 
Sbjct: 2   SNDNKKVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFE 61

Query: 154 GPQHATRVFDMIDDFYVGDL 173
              H+T    M+D++YVGD+
Sbjct: 62  DVGHSTTARAMMDEYYVGDI 81

[Close]

BLAST of CmaCh12G012710 vs. Swiss-Prot
Match: CYB5A_ARATH (Cytochrome b5 isoform A OS=Arabidopsis thaliana GN=CB5-A PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 5.3e-15
Identity = 33/76 (43.42%), Postives = 55/76 (72.37%), Query Frame = 1


Query: 98  SKVYGKDEVSVHNKRTDCWIIIKNKVYDVTSYVEEHPGG-DAILAHAGDDSTEGFYGPQH 157
           +K+Y  +E + HNK+ DCW++I  KVYDV+SY++EHPGG D +LA AG D+T+ F    H
Sbjct: 5   TKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGH 64

Query: 158 ATRVFDMIDDFYVGDL 173
           +    ++++ +++G+L
Sbjct: 65  SKDARELMEKYFIGEL 80

[Close]

BLAST of CmaCh12G012710 vs. Swiss-Prot
Match: DES6_PHYPA (Acyl-lipid (9-3)-desaturase OS=Physcomitrella patens subsp. patens GN=DES6 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 6.9e-15
Identity = 34/68 (50.00%), Postives = 49/68 (72.06%), Query Frame = 1


Query: 105 EVSVHNKRTDCWIIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMI 164
           EV+VHNK +DCWI++KNKVYDV+++ +EHPGG  I  + G D T+ F    HA   + ++
Sbjct: 109 EVAVHNKPSDCWIVVKNKVYDVSNFADEHPGGSVISTYFGRDGTDVF-SSFHAASTWKIL 168

Query: 165 DDFYVGDL 173
            DFY+GD+
Sbjct: 169 QDFYIGDV 175

[Close]

BLAST of CmaCh12G012710 vs. Swiss-Prot
Match: CYB5_TOBAC (Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.5e-14
Identity = 35/76 (46.05%), Postives = 52/76 (68.42%), Query Frame = 1


Query: 98  SKVYGKDEVSVHNKRTDCWIIIKNKVYDVTSYVEEHPGGDAILAHA-GDDSTEGFYGPQH 157
           +KV+   EVS HN   DCW++I  KVYDVT ++++HPGGD +L  A G D+T+ F    H
Sbjct: 5   TKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFEDVGH 64

Query: 158 ATRVFDMIDDFYVGDL 173
           ++    M+D++YVGD+
Sbjct: 65  SSSARAMLDEYYVGDI 80

[Close]

BLAST of CmaCh12G012710 vs. TrEMBL
Match: A0A0A0LFU2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G000900 PE=3 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 9.3e-51
Identity = 98/122 (80.33%), Postives = 113/122 (92.62%), Query Frame = 1


Query: 54  VHSELYKMMIIVAALVLGVLFGALILIPRDRNAQKVQLNSTNKASKVY-GKDEVSVHNKR 113
           ++ +++KM+I++AAL+L VL GAL LIPRDRNAQKVQLN  NK+SKVY  KDEVSVHNKR
Sbjct: 114 MNHQVHKMVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSKDEVSVHNKR 173

Query: 114 TDCWIIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDL 173
           TDCW+IIKN+VYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHATRVFDMI+DFY+GDL
Sbjct: 174 TDCWVIIKNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDL 233

Query: 174 RL 175
           +L
Sbjct: 234 KL 235

[Close]

BLAST of CmaCh12G012710 vs. TrEMBL
Match: A0A067FGQ3_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g036360mg PE=3 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 4.6e-42
Identity = 86/117 (73.50%), Postives = 98/117 (83.76%), Query Frame = 1


Query: 61  MMIIVAALVLGVLFGALILIPRDRNAQKVQLN----STNKASKVYGKDEVSVHNKRTDCW 120
           M+IIVAAL+LG + G  I IPR   +   +      ++NKASK+Y KDEV++HNKR DCW
Sbjct: 1   MVIIVAALILGAILGVFIFIPRHPTSGHSKSTHSSANSNKASKIYSKDEVTLHNKRDDCW 60

Query: 121 IIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLR 174
           IIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFY+GDL+
Sbjct: 61  IIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYIGDLK 117

[Close]

BLAST of CmaCh12G012710 vs. TrEMBL
Match: V4UPZ7_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10009944mg PE=3 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 6.1e-42
Identity = 86/117 (73.50%), Postives = 97/117 (82.91%), Query Frame = 1


Query: 61  MMIIVAALVLGVLFGALILIPRD----RNAQKVQLNSTNKASKVYGKDEVSVHNKRTDCW 120
           M+IIVAAL+LG + G  I IPR      +       ++NKASK+Y KDEV++HNKR DCW
Sbjct: 1   MVIIVAALILGAILGVFIFIPRHPASGHSKSTHSSANSNKASKIYSKDEVTLHNKRDDCW 60

Query: 121 IIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLR 174
           IIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFY+GDL+
Sbjct: 61  IIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYIGDLK 117

[Close]

BLAST of CmaCh12G012710 vs. TrEMBL
Match: A0A061GU94_THECC (Cytochrome B5-like protein, n5,ATCB5LP,CB5LP OS=Theobroma cacao GN=TCM_037790 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 3.0e-41
Identity = 89/117 (76.07%), Postives = 97/117 (82.91%), Query Frame = 1


Query: 61  MMIIVAALVLGVLFGALILIPR---DRNAQKVQLNSTN-KASKVYGKDEVSVHNKRTDCW 120
           M I V AL+LGVL GA ILIPR     +  +VQ N  N KASK Y K EV++HNKRTDCW
Sbjct: 1   MAIAVIALILGVLLGAFILIPRHGKSAHTNQVQSNGNNSKASKSYSKTEVALHNKRTDCW 60

Query: 121 IIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLR 174
           IIIK+KVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHAT VFDMIDDFY+GDL+
Sbjct: 61  IIIKDKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATSVFDMIDDFYIGDLQ 117

[Close]

BLAST of CmaCh12G012710 vs. TrEMBL
Match: A0A0B0P428_GOSAR (Cytochrome b5 OS=Gossypium arboreum GN=F383_01668 PE=3 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 3.3e-40
Identity = 86/117 (73.50%), Postives = 98/117 (83.76%), Query Frame = 1


Query: 61  MMIIVAALVLGVLFGALILIPRD-RNAQKVQLNST---NKASKVYGKDEVSVHNKRTDCW 120
           M I + AL+L VL GA IL+PR  ++A K ++ ST   +KASK Y K EV +HNKRTDCW
Sbjct: 1   MEIAIIALILAVLLGAFILVPRHGKSAHKNKVKSTVANSKASKSYSKTEVMLHNKRTDCW 60

Query: 121 IIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLR 174
           IIIK+KVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATR FDMIDDFY+GDL+
Sbjct: 61  IIIKDKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRTFDMIDDFYIGDLQ 117

[Close]

BLAST of CmaCh12G012710 vs. TAIR10
Match: AT1G60660.1 (AT1G60660.1 cytochrome B5-like protein)

HSP 1 Score: 147.1 bits (370), Expect = 9.9e-36
Identity = 75/120 (62.50%), Postives = 89/120 (74.17%), Query Frame = 1


Query: 62  MIIVAALVLGVLFGALILIP----RDRNAQKVQLNSTNKASKV-----YGKDEVSVHNKR 121
           MI V  L+LG L  AL LI     R  + Q+ + +S+     V     Y K EV+VHNKR
Sbjct: 1   MIAVIGLLLGFLVSALFLIQGKRRRTNDNQEKKRSSSEPVEDVVRPKSYSKSEVAVHNKR 60

Query: 122 TDCWIIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDL 173
            DCWIIIK+KVYD+TSYVEEHPGGDAIL HAGDDST+GF+GPQHATRVFDMI+DFY+G+L
Sbjct: 61  NDCWIIIKDKVYDITSYVEEHPGGDAILDHAGDDSTDGFFGPQHATRVFDMIEDFYIGEL 120

[Close]

BLAST of CmaCh12G012710 vs. TAIR10
Match: AT1G26340.1 (AT1G26340.1 cytochrome B5 isoform A)

HSP 1 Score: 82.4 bits (202), Expect = 3.0e-16
Identity = 33/76 (43.42%), Postives = 55/76 (72.37%), Query Frame = 1


Query: 98  SKVYGKDEVSVHNKRTDCWIIIKNKVYDVTSYVEEHPGG-DAILAHAGDDSTEGFYGPQH 157
           +K+Y  +E + HNK+ DCW++I  KVYDV+SY++EHPGG D +LA AG D+T+ F    H
Sbjct: 5   TKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGH 64

Query: 158 ATRVFDMIDDFYVGDL 173
           +    ++++ +++G+L
Sbjct: 65  SKDARELMEKYFIGEL 80

[Close]

BLAST of CmaCh12G012710 vs. TAIR10
Match: AT5G48810.1 (AT5G48810.1 cytochrome B5 isoform D)

HSP 1 Score: 79.3 bits (194), Expect = 2.5e-15
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 1


Query: 99  KVYGKDEVSVHNKRTDCWIIIKNKVYDVTSYVEEHPGGD-AILAHAGDDSTEGFYGPQHA 158
           KV+   EVS H+   DCWI+I  KVYDVT ++++HPGGD  IL   G D+T+ F    H+
Sbjct: 6   KVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHS 65

Query: 159 TRVFDMIDDFYVGDL 173
           +    M+D++YVGD+
Sbjct: 66  STAKAMLDEYYVGDI 80

[Close]

BLAST of CmaCh12G012710 vs. TAIR10
Match: AT5G53560.1 (AT5G53560.1 cytochrome B5 isoform E)

HSP 1 Score: 78.6 bits (192), Expect = 4.3e-15
Identity = 34/75 (45.33%), Postives = 50/75 (66.67%), Query Frame = 1


Query: 99  KVYGKDEVSVHNKRTDCWIIIKNKVYDVTSYVEEHPGGD-AILAHAGDDSTEGFYGPQHA 158
           KV   +EVS HNK  DCW+II  KVYDVT ++++HPGGD  +L+  G D+T  F    H+
Sbjct: 6   KVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVGHS 65

Query: 159 TRVFDMIDDFYVGDL 173
               DM+D +++G++
Sbjct: 66  DTARDMMDKYFIGEI 80

[Close]

BLAST of CmaCh12G012710 vs. TAIR10
Match: AT2G32720.1 (AT2G32720.1 cytochrome B5 isoform B)

HSP 1 Score: 77.0 bits (188), Expect = 1.3e-14
Identity = 32/76 (42.11%), Postives = 52/76 (68.42%), Query Frame = 1


Query: 98  SKVYGKDEVSVHNKRTDCWIIIKNKVYDVTSYVEEHPGG-DAILAHAGDDSTEGFYGPQH 157
           +K++   EVS HN+  DCWI+I  KVY+VT ++E+HPGG D +L+  G D+T+ F    H
Sbjct: 5   AKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGH 64

Query: 158 ATRVFDMIDDFYVGDL 173
           +    +M++ +YVG++
Sbjct: 65  SESAREMMEQYYVGEI 80

[Close]

BLAST of CmaCh12G012710 vs. NCBI nr
Match: gi|700205464|gb|KGN60583.1| (hypothetical protein Csa_2G000900 [Cucumis sativus])

HSP 1 Score: 208.0 bits (528), Expect = 1.3e-50
Identity = 98/122 (80.33%), Postives = 113/122 (92.62%), Query Frame = 1


Query: 54  VHSELYKMMIIVAALVLGVLFGALILIPRDRNAQKVQLNSTNKASKVY-GKDEVSVHNKR 113
           ++ +++KM+I++AAL+L VL GAL LIPRDRNAQKVQLN  NK+SKVY  KDEVSVHNKR
Sbjct: 114 MNHQVHKMVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSKDEVSVHNKR 173

Query: 114 TDCWIIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDL 173
           TDCW+IIKN+VYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHATRVFDMI+DFY+GDL
Sbjct: 174 TDCWVIIKNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDL 233

Query: 174 RL 175
           +L
Sbjct: 234 KL 235

[Close]

BLAST of CmaCh12G012710 vs. NCBI nr
Match: gi|659071233|ref|XP_008458811.1| (PREDICTED: cytochrome b5 [Cucumis melo])

HSP 1 Score: 204.5 bits (519), Expect = 1.5e-49
Identity = 98/115 (85.22%), Postives = 107/115 (93.04%), Query Frame = 1


Query: 61  MMIIVAALVLGVLFGALILIPRDRNAQKVQLNSTNKASKVY-GKDEVSVHNKRTDCWIII 120
           M+I++AAL+L VL GAL LIPRDRNAQKVQLN  NK+SKVY  KDEVSVHNKRTDCW+II
Sbjct: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSKDEVSVHNKRTDCWVII 60

Query: 121 KNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 175
           KNKVYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHATRVFDMI+DFY+GDL+L
Sbjct: 61  KNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 115

[Close]

BLAST of CmaCh12G012710 vs. NCBI nr
Match: gi|449446520|ref|XP_004141019.1| (PREDICTED: cytochrome B5-like protein [Cucumis sativus])

HSP 1 Score: 203.4 bits (516), Expect = 3.3e-49
Identity = 97/115 (84.35%), Postives = 107/115 (93.04%), Query Frame = 1


Query: 61  MMIIVAALVLGVLFGALILIPRDRNAQKVQLNSTNKASKVY-GKDEVSVHNKRTDCWIII 120
           M+I++AAL+L VL GAL LIPRDRNAQKVQLN  NK+SKVY  KDEVSVHNKRTDCW+II
Sbjct: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSKDEVSVHNKRTDCWVII 60

Query: 121 KNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 175
           KN+VYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHATRVFDMI+DFY+GDL+L
Sbjct: 61  KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 115

[Close]

BLAST of CmaCh12G012710 vs. NCBI nr
Match: gi|641847695|gb|KDO66574.1| (hypothetical protein CISIN_1g036360mg [Citrus sinensis])

HSP 1 Score: 179.1 bits (453), Expect = 6.7e-42
Identity = 86/117 (73.50%), Postives = 98/117 (83.76%), Query Frame = 1


Query: 61  MMIIVAALVLGVLFGALILIPRDRNAQKVQLN----STNKASKVYGKDEVSVHNKRTDCW 120
           M+IIVAAL+LG + G  I IPR   +   +      ++NKASK+Y KDEV++HNKR DCW
Sbjct: 1   MVIIVAALILGAILGVFIFIPRHPTSGHSKSTHSSANSNKASKIYSKDEVTLHNKRDDCW 60

Query: 121 IIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLR 174
           IIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFY+GDL+
Sbjct: 61  IIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYIGDLK 117

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BLAST of CmaCh12G012710 vs. NCBI nr
Match: gi|567918576|ref|XP_006451294.1| (hypothetical protein CICLE_v10009944mg [Citrus clementina])

HSP 1 Score: 178.7 bits (452), Expect = 8.7e-42
Identity = 86/117 (73.50%), Postives = 97/117 (82.91%), Query Frame = 1


Query: 61  MMIIVAALVLGVLFGALILIPRD----RNAQKVQLNSTNKASKVYGKDEVSVHNKRTDCW 120
           M+IIVAAL+LG + G  I IPR      +       ++NKASK+Y KDEV++HNKR DCW
Sbjct: 1   MVIIVAALILGAILGVFIFIPRHPASGHSKSTHSSANSNKASKIYSKDEVTLHNKRDDCW 60

Query: 121 IIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLR 174
           IIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFY+GDL+
Sbjct: 61  IIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYIGDLK 117

The following BLAST results are available for this feature:

BLAST of CmaCh12G012710 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CYP5F_ARATH	1.8e-34	62.50	Cytochrome B5-like protein OS=Arabidopsis thaliana GN=CB5LP PE=2 SV=1	[more]
CYB5_ORYSJ	4.1e-15	45.00	Cytochrome b5 OS=Oryza sativa subsp. japonica GN=Os05g0108800 PE=2 SV=2	[more]
CYB5A_ARATH	5.3e-15	43.42	Cytochrome b5 isoform A OS=Arabidopsis thaliana GN=CB5-A PE=1 SV=1	[more]
DES6_PHYPA	6.9e-15	50.00	Acyl-lipid (9-3)-desaturase OS=Physcomitrella patens subsp. patens GN=DES6 PE=1 ...	[more]
CYB5_TOBAC	1.5e-14	46.05	Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1	[more]

BLAST of CmaCh12G012710 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LFU2_CUCSA	9.3e-51	80.33	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G000900 PE=3 SV=1	[more]
A0A067FGQ3_CITSI	4.6e-42	73.50	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g036360mg PE=3 SV=1	[more]
V4UPZ7_9ROSI	6.1e-42	73.50	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10009944mg PE=3 SV=1	[more]
A0A061GU94_THECC	3.0e-41	76.07	Cytochrome B5-like protein, n5,ATCB5LP,CB5LP OS=Theobroma cacao GN=TCM_037790 PE...	[more]
A0A0B0P428_GOSAR	3.3e-40	73.50	Cytochrome b5 OS=Gossypium arboreum GN=F383_01668 PE=3 SV=1	[more]

BLAST of CmaCh12G012710 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G60660.1	9.9e-36	62.50	cytochrome B5-like protein	[more]
AT1G26340.1	3.0e-16	43.42	cytochrome B5 isoform A	[more]
AT5G48810.1	2.5e-15	46.67	cytochrome B5 isoform D	[more]
AT5G53560.1	4.3e-15	45.33	cytochrome B5 isoform E	[more]
AT2G32720.1	1.3e-14	42.11	cytochrome B5 isoform B	[more]

BLAST of CmaCh12G012710 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700205464\|gb\|KGN60583.1\|	1.3e-50	80.33	hypothetical protein Csa_2G000900 [Cucumis sativus]	[more]
gi\|659071233\|ref\|XP_008458811.1\|	1.5e-49	85.22	PREDICTED: cytochrome b5 [Cucumis melo]	[more]
gi\|449446520\|ref\|XP_004141019.1\|	3.3e-49	84.35	PREDICTED: cytochrome B5-like protein [Cucumis sativus]	[more]
gi\|641847695\|gb\|KDO66574.1\|	6.7e-42	73.50	hypothetical protein CISIN_1g036360mg [Citrus sinensis]	[more]
gi\|567918576\|ref\|XP_006451294.1\|	8.7e-42	73.50	hypothetical protein CICLE_v10009944mg [Citrus clementina]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001199	Cyt_B5-like_heme/steroid-bd
IPR018506	Cyt_B5_heme-BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006040	amino sugar metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0016126	sterol biosynthetic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004128	cytochrome-b5 reductase activity, acting on NAD(P)H
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh12G012710.1	CmaCh12G012710.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 123..133 score: 2.3E-5coord: 160..172 score: 2.3E-5coord: 133..147 score: 2.
IPR001199	Cytochrome b5-like heme/steroid binding domain	GENE3D	G3DSA:3.10.120.10		coord: 98..173 score: 8.9
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 104..172 score: 1.9
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 101..173 score: 1.4
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROFILE	PS50255	CYTOCHROME_B5_2	coord: 98..173 score: 24
IPR001199	Cytochrome b5-like heme/steroid binding domain	unknown	SSF55856	Cytochrome b5-like heme/steroid binding domain	coord: 96..172 score: 3.79
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 129..136 scor
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 61..173 score: 7.0
None	No IPR available	PANTHER	PTHR19359:SF10	SUBFAMILY NOT NAMED	coord: 61..173 score: 7.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh12G012710	Cucsa.116640	Cucumber (Gy14) v1	cgycmaB0285
CmaCh12G012710	Cla016796	Watermelon (97103) v1	cmawmB172
CmaCh12G012710	Csa2G000900	Cucumber (Chinese Long) v2	cmacuB173
CmaCh12G012710	MELO3C004588	Melon (DHL92) v3.5.1	cmameB172
CmaCh12G012710	ClCG11G002140	Watermelon (Charleston Gray)	cmawcgB147
CmaCh12G012710	CSPI02G00840	Wild cucumber (PI 183967)	cmacpiB174
CmaCh12G012710	CmoCh12G013050	Cucurbita moschata (Rifu)	cmacmoB177
CmaCh12G012710	Lsi11G002270	Bottle gourd (USVL1VR-Ls)	cmalsiB159
CmaCh12G012710	Cp4.1LG07g10270	Cucurbita pepo (Zucchini)	cmacpeB204
CmaCh12G012710	MELO3C004588.2	Melon (DHL92) v3.6.1	cmamedB187
CmaCh12G012710	CsaV3_2G001920	Cucumber (Chinese Long) v3	cmacucB0202
CmaCh12G012710	Cla97C11G208530	Watermelon (97103) v2	cmawmbB171
CmaCh12G012710	Bhi06G001461	Wax gourd	cmawgoB0189
CmaCh12G012710	CsGy2G000810	Cucumber (Gy14) v2	cgybcmaB178
CmaCh12G012710	Carg18596	Silver-seed gourd	carcmaB1236

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh12G012710	Cucumber (Gy14) v2	cgybcmaB055
CmaCh12G012710	Melon (DHL92) v3.6.1	cmamedB165
CmaCh12G012710	Silver-seed gourd	carcmaB0048
CmaCh12G012710	Silver-seed gourd	carcmaB0121
CmaCh12G012710	Silver-seed gourd	carcmaB0797
CmaCh12G012710	Watermelon (97103) v2	cmawmbB179
CmaCh12G012710	Watermelon (97103) v2	cmawmbB192
CmaCh12G012710	Wax gourd	cmawgoB0234
CmaCh12G012710	Cucurbita maxima (Rimu)	cmacmaB086
CmaCh12G012710	Cucurbita maxima (Rimu)	cmacmaB122
CmaCh12G012710	Cucurbita maxima (Rimu)	cmacmaB190
CmaCh12G012710	Cucumber (Gy14) v1	cgycmaB0813
CmaCh12G012710	Cucurbita moschata (Rifu)	cmacmoB169
CmaCh12G012710	Cucurbita moschata (Rifu)	cmacmoB171
CmaCh12G012710	Cucurbita moschata (Rifu)	cmacmoB186
CmaCh12G012710	Cucurbita moschata (Rifu)	cmacmoB190
CmaCh12G012710	Wild cucumber (PI 183967)	cmacpiB158
CmaCh12G012710	Cucumber (Chinese Long) v2	cmacuB160
CmaCh12G012710	Melon (DHL92) v3.5.1	cmameB152
CmaCh12G012710	Watermelon (Charleston Gray)	cmawcgB158
CmaCh12G012710	Watermelon (97103) v1	cmawmB157
CmaCh12G012710	Cucurbita pepo (Zucchini)	cmacpeB171
CmaCh12G012710	Cucurbita pepo (Zucchini)	cmacpeB184
CmaCh12G012710	Cucurbita pepo (Zucchini)	cmacpeB196
CmaCh12G012710	Bottle gourd (USVL1VR-Ls)	cmalsiB179