Cucsa.108190 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.108190 vs. Swiss-Prot
Match: PRIPO_DANRE (DNA-directed primase/polymerase protein OS=Danio rerio GN=primpol PE=2 SV=2)

HSP 1 Score: 243.4 bits (620), Expect = 6.1e-63
Identity = 173/488 (35.45%), Postives = 234/488 (47.95%), Query Frame = 1

Query: 48  VEETEENASSIQDTVEKFSPIVFYGSPRGVPPKRPSSLWRLLREIRVDLSEQSRFKLSKQ 107
           V+  E+ ASS Q      SP+     PR   P +PSS+WRL                   
Sbjct: 10  VKSVEQRASSFQS-----SPLSCPYKPRLSQPWQPSSIWRL------------------- 69

Query: 108 VWATFPRQEEAIKFSRE-HTDVHIFSYQDHFN--GQRRFLVSSYSEFWHRYKSMDAKCRH 167
               FPRQ  AI F++    DVHIFS +   +  GQR FLV+SYSE WH Y +      H
Sbjct: 70  ----FPRQNAAIAFAQHIKQDVHIFSLEKEGSDAGQRIFLVTSYSELWHYYSTHRHSLMH 129

Query: 168 HYEVIQEGAPCHLYFDLEYSKRINTGKNGDEMVDSLISVVLQALNEKYSIQGSFDWVLEL 227
            YEVI EGA C LYFDLE+ K  N   +G  MV  LI  V + L E Y +  S   VL+L
Sbjct: 130 CYEVILEGAVCKLYFDLEFHKASNKNLDGKMMVAKLIQYVCEKLEEVYGLHCSAKDVLDL 189

Query: 228 DSSNEEKFSRHLIIRVPKVAFKDNSHAGAFVGEICSRICS--------AKVEGRYEELFI 287
           DSS  +KFS HLI  +P  AFKDNSH G F+ +I     +        A  E R +    
Sbjct: 190 DSSTSDKFSHHLIFMLPNAAFKDNSHVGRFIHDILHPALTNLKKSNPEAPGENRDDVEGT 249

Query: 288 KKDSSSTER-------------PSHLFVDNAVYSRNRCFRLALSSKAGKTSVLL------ 347
           +     TE                 LFVD  VY++NR FRL  SSK GK +  +      
Sbjct: 250 QAKRRKTEENDLGFLTVKNEKGQEQLFVDLGVYTKNRNFRLYKSSKLGKNAAFIVAEDNK 309

Query: 348 --PTGRHACTKMCEEDMFMASLICNVDADCEKLLVCKMDLDCIKTLQFEMEEKCNFGQNF 407
             P      TK  +E +F+ASLI NV    +++L             ++M +K   G   
Sbjct: 310 FVPNPSKQITK--DERIFLASLITNVSFTGQRILT------------YDMTQKSTAGSEC 369

Query: 408 SFTKEAVLTGSMRDFSATSCMG---ISPFPDVDKFVQSVASNGNVAGKIRCWYWFSEFGL 467
              +        R+  ++  +G    SPF +VD+FV ++     + G IR W +F+   L
Sbjct: 370 PTLE--------RESHSSDLLGDQKTSPFKEVDEFVLTLVCKDGIQGSIRRWNYFACEQL 429

Query: 468 IVYSMSSNRYCERIGREHKSNHVMYVVDLRRAAYYQKCHDPDCR--GYRSPLRPIPIDAI 499
           +VY +   R+C  + R HKSN+++ VVDL+   +YQKCHDP+CR   YRS   P+P +  
Sbjct: 430 LVYDIEKFRWCHNVKRFHKSNNIIIVVDLKEEVWYQKCHDPECRRQNYRSSSFPLPQEVC 447

BLAST of Cucsa.108190 vs. Swiss-Prot
Match: PRIPO_MOUSE (DNA-directed primase/polymerase protein OS=Mus musculus GN=Primpol PE=2 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 6.9e-51
Identity = 161/488 (32.99%), Postives = 229/488 (46.93%), Query Frame = 1

Query: 48  VEETEENASSIQDTVEKFSPIVFYGSPRGVPPKRPSSLWRLLREIRVDLSEQSRFKLSKQ 107
           V++ EE AS  +       P+     PR   P+ PSS+W+L                   
Sbjct: 9   VKQIEERASHYER-----KPLSSVYRPRLAKPEEPSSIWKL------------------- 68

Query: 108 VWATFPRQEEAIKFSREHTD-VHIFSYQ-DHFNGQRRFLVSSYSEFWHRYKSMDAKCRHH 167
               F RQ +A  F +   + VH+F+ +    NGQR +LV+SY++ W  YK+      H 
Sbjct: 69  ----FHRQNQAFNFVKSCKESVHVFALECKRGNGQRIYLVTSYAQLWFYYKTRKTLL-HC 128

Query: 168 YEVIQEGAPCHLYFDLEYSKRINTGKNGDEMVDSLISVVLQALNEKYSIQGSFDWVLELD 227
           YEVI E A C LYFDLE++K  N G +G  MV  LI  V +AL E Y++Q S + V  LD
Sbjct: 129 YEVIPENAVCKLYFDLEFNKLANPGADGKMMVALLIQHVCKALEEFYNVQCSAEDVFNLD 188

Query: 228 SSNEEKFSRHLIIRVPKVA-----------------------FKDNSHAGAFVGEICSRI 287
           SS EEKFSRHLI ++  VA                         D +     VG+  S  
Sbjct: 189 SSTEEKFSRHLIFQLHNVAFKDNRHAGNFVRKILQPALHLIAEDDEAKVPEAVGQDASGF 248

Query: 288 CSAKVEGRYEELFIK--------KDSSSTERPSH-----LFVDNAVYSRNRCFRLALSSK 347
               ++    E   K         D S     +H     LFVD  VY++NR FRL  SSK
Sbjct: 249 SVTPLKQEISEAREKVGLPKQCDPDLSFLVVKNHMGEKCLFVDLGVYTKNRNFRLYQSSK 308

Query: 348 AGK-TSVLLPTGRHACTKMC-----EEDMFMASLICNVD-ADCEKLLVCKMDLDCIKTLQ 407
            GK  S+ +        K       E   F++SL+ NV  +D  ++L C           
Sbjct: 309 IGKCVSLEVAEDNRFIPKQSKDISEENQYFLSSLVSNVRFSDTLRVLTCHPS-------- 368

Query: 408 FEMEEKCNFGQNFSFTKEAVLTGSMRDFSATSCMGISPFPDVDKFVQSVASNGNVAGKIR 467
              + K    + F+ T  +V   S+  F        SP+P+VD+FV S+ +  ++ G IR
Sbjct: 369 ---QTKRKRAECFNSTGTSV--ESIEGFQG------SPYPEVDQFVLSLVNKHDIKGGIR 428

Query: 468 CWYWFSEFGLIVYSMSSNRYCERIGREHKSNHVMYVVDLRRAAYYQKCHDPDCR--GYRS 489
            W +F    L+VY +   R+CE IGR HKSN++M +VDL+   +YQKCHDP C+   ++S
Sbjct: 429 RWNYFFPEELLVYDICKYRWCENIGRAHKSNNIMILVDLKNEVWYQKCHDPVCKAQNFKS 448

BLAST of Cucsa.108190 vs. Swiss-Prot
Match: PRIPO_HUMAN (DNA-directed primase/polymerase protein OS=Homo sapiens GN=PRIMPOL PE=1 SV=2)

HSP 1 Score: 134.8 bits (338), Expect = 3.0e-30
Identity = 86/205 (41.95%), Postives = 116/205 (56.59%), Query Frame = 1

Query: 93  RVDLSEQSRFKLSK-----QVWATFPRQEEAIKFSRE-HTDVHIFSYQDHF-NGQRRFLV 152
           R  LS   R +LSK      +W  F RQ +A  F +    DVH+F+ +    +GQR +LV
Sbjct: 21  RKPLSSVYRPRLSKPEEPPSIWRLFHRQAQAFNFVKSCKEDVHVFALECKVGDGQRIYLV 80

Query: 153 SSYSEFWHRYKSMDAKCRHHYEVIQEGAPCHLYFDLEYSKRINTGKNGDEMVDSLISVVL 212
           ++Y+EFW  YKS      H YEVI E A C LYFDLE++K  N G +G +MV  LI  V 
Sbjct: 81  TTYAEFWFYYKSRK-NLLHCYEVIPENAVCKLYFDLEFNKPANPGADGKKMVALLIEYVC 140

Query: 213 QALNEKYSIQGSFDWVLELDSSNEEKFSRHLIIRVPKVAFKDNSHAGAFVGEICSRICSA 272
           +AL E Y +  S + VL LDSS +EKFS+HLI ++  VAFKDN H G F+ +I       
Sbjct: 141 KALQELYGVNCSAEDVLNLDSSTDEKFSQHLIFQLHDVAFKDNIHVGNFLRKI------- 200

Query: 273 KVEGRYEELFIKKDSSSTERPSHLF 291
            ++   + L  + D S+ E   H F
Sbjct: 201 -LQPALDLLGSEDDDSAPETTGHGF 216

HSP 2 Score: 118.2 bits (295), Expect = 2.9e-25
Identity = 83/284 (29.23%), Postives = 133/284 (46.83%), Query Frame = 1

Query: 288 HLFVDNAVYSRNRCFRLALSSKAGKTSVLLPTGRHACTKMCEEDM------FMASLICNV 347
           HLFVD  VY+RNR FRL  SSK GK   L  T  +    +  +D+      F++SL+ NV
Sbjct: 276 HLFVDLGVYTRNRNFRLYKSSKIGKRVALEVTEDNKFFPIQSKDVSDEYQYFLSSLVSNV 335

Query: 348 D-ADCEKLLVCKMDLDCIKTLQFEMEEKCNFGQNFSFTKEAVLTGSMRDFSATSCMGISP 407
             +D  ++L C+   +  K + +             F        ++  F        SP
Sbjct: 336 RFSDTLRILTCEPSQNKQKGVGY-------------FNSIGTSVETIEGFQC------SP 395

Query: 408 FPDVDKFVQSVASNGNVAGKIRCWYWFSEFGLIVYSMSSNRYCERIGREHKSNHVMYVVD 467
           +P+VD FV S+ +   + G IR W +F    L+VY +   R+CE IGR HKSN++M +VD
Sbjct: 396 YPEVDHFVLSLVNKDGIKGGIRRWNYFFPEELLVYDICKYRWCENIGRAHKSNNIMILVD 455

Query: 468 LRRAAYYQKCHDPDCR--GYRSPLRPIPIDAIPSPWISTDSGQISNDMVSTHDCIDYHQS 527
           L+   +YQKCHDP C+   ++S   P+P +         +    +++   T      +  
Sbjct: 456 LKNEVWYQKCHDPVCKAENFKSDCFPLPAEVCLLFLFKEEEEFTTDEADETRSNETQNPH 515

Query: 528 PSNDENCLLLFSDKNITDSSVKDSWWLEVMRVADDVENKRNMVL 563
             +        S   + D+ + D+++LE    A+  E   N +L
Sbjct: 516 KPSPSRLSTGASADAVWDNGIDDAYFLEATEDAELAEAAENSLL 540

BLAST of Cucsa.108190 vs. Swiss-Prot
Match: PRIPO_BOVIN (DNA-directed primase/polymerase protein OS=Bos taurus GN=PRIMPOL PE=2 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 1.5e-29
Identity = 80/173 (46.24%), Postives = 105/173 (60.69%), Query Frame = 1

Query: 93  RVDLSEQSRFKLSK-----QVWATFPRQEEAIKFSRE-HTDVHIFSYQDHF-NGQRRFLV 152
           R  LS   R +LSK      +W  F RQ +A  F +    +VH+F+ +    +GQR +LV
Sbjct: 21  RKPLSSVYRPRLSKPEEPPSIWKLFHRQTQAFNFVKSCKQEVHVFALECKVGDGQRIYLV 80

Query: 153 SSYSEFWHRYKSMDAKCRHHYEVIQEGAPCHLYFDLEYSKRINTGKNGDEMVDSLISVVL 212
           ++Y++ W  YKS      H YEVI E A C LYFDLE++K  N G +G +MV  LI  V 
Sbjct: 81  TTYTQLWFYYKSR-RNLLHCYEVIPENAVCKLYFDLEFNKLANPGADGKKMVALLIEHVC 140

Query: 213 QALNEKYSIQGSFDWVLELDSSNEEKFSRHLIIRVPKVAFKDNSHAGAFVGEI 259
           +AL E Y++  S + VL LDSS EEKFSRHLI ++  VAFKDN H G FV +I
Sbjct: 141 KALQEFYTVNCSAEDVLNLDSSTEEKFSRHLIFQLHDVAFKDNIHVGNFVRKI 192

HSP 2 Score: 120.2 bits (300), Expect = 7.7e-26
Identity = 93/300 (31.00%), Postives = 142/300 (47.33%), Query Frame = 1

Query: 274 LFIKKDSSSTERPSHLFVDNAVYSRNRCFRLALSSKAGKTSVL--------LPTGRHACT 333
           L +K D         LFVD  VY+RNR FRL  SSK GK   L         P      +
Sbjct: 266 LIVKNDMGE----KRLFVDLGVYTRNRNFRLYKSSKIGKYVALEVAEDNKFFPIQSKNIS 325

Query: 334 KMCEEDMFMASLICNVD-ADCEKLLVCKMDLDCIKTLQFEMEEKCNFGQNFSFTKEAVLT 393
           K  E   F++SL+ NV  +D  ++L C +        Q +   +C     FS T  +V  
Sbjct: 326 K--ENQYFLSSLVSNVRFSDALRILTCDVP-------QSKQRVQC-----FSRTGTSV-- 385

Query: 394 GSMRDFSATSCMGISPFPDVDKFVQSVASNGNVAGKIRCWYWFSEFGLIVYSMSSNRYCE 453
                  A      SP+P++D+FV S+ +   + G IR W +F    L+VY +   R+CE
Sbjct: 386 ------EAIEGFQCSPYPEIDQFVLSLVNKNGIKGGIRRWNYFFPEELLVYDICKYRWCE 445

Query: 454 RIGREHKSNHVMYVVDLRRAAYYQKCHDPDCR--GYRSPLRPIPIDAIPSPWISTDSGQI 513
            IGR H+SN++M +VDL+   +YQKCHDP C+   ++S   P+P +      +  +  + 
Sbjct: 446 NIGRAHRSNNIMILVDLKNEVWYQKCHDPVCKAENFKSDCFPLPAEVCLLSLLK-EGEEF 505

Query: 514 SNDMVSTHDCIDYHQSPSNDENCLLLFSDKNITDSSVKDSWWLEVMRVADDVENKRNMVL 563
           + D  +  +    H  PS+       FSD +  D+ + D++ LE    A+  E   N +L
Sbjct: 506 TTDTTADTETKSPH-GPSSSVLSKGAFSDAD-WDNGIDDTYILEATEDAELAEAAENSLL 536

BLAST of Cucsa.108190 vs. TrEMBL
Match: A0A0A0L6Q1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G184000 PE=4 SV=1)

HSP 1 Score: 1189.9 bits (3077), Expect = 0.0e+00
Identity = 579/597 (96.98%), Postives = 580/597 (97.15%), Query Frame = 1

Query: 2   DDVDRLFECFKCGVSPPQSAVRRKKKRKSKVKPECSVNFETPVSGPVEETEENASSIQDT 61
           DDVDRLFECFKCGVSPPQSAVRRKKKRKSKVKPECSVNFETPVSGPVE+ EENASSIQDT
Sbjct: 7   DDVDRLFECFKCGVSPPQSAVRRKKKRKSKVKPECSVNFETPVSGPVEKAEENASSIQDT 66

Query: 62  VEK-------------FSPIVFYGSPRGVPPKRPSSLWRLLREIRVDLSEQSRFKLSKQV 121
           VEK             FSPIVFYGSPRGVPPKRPSSLWRLLREIRVDLSEQSRFKLSKQV
Sbjct: 67  VEKIGLTSKDKGSRSKFSPIVFYGSPRGVPPKRPSSLWRLLREIRVDLSEQSRFKLSKQV 126

Query: 122 WATFPRQEEAIKFSREHTDVHIFSYQDHFNGQRRFLVSSYSEFWHRYKSMDAKCRHHYEV 181
           WATFPRQEEAIKFSREHTDVHIFSYQDHFNGQRRFLVSSYSEFWHRYKSMDAKCRHHYEV
Sbjct: 127 WATFPRQEEAIKFSREHTDVHIFSYQDHFNGQRRFLVSSYSEFWHRYKSMDAKCRHHYEV 186

Query: 182 IQEGAPCHLYFDLEYSKRINTGKNGDEMVDSLISVVLQALNEKYSIQGSFDWVLELDSSN 241
           IQEGAPCHLYFDLEYSKRINTGKNGDEMVDSLISVVLQALNEKYSIQGSFDWVLELDSSN
Sbjct: 187 IQEGAPCHLYFDLEYSKRINTGKNGDEMVDSLISVVLQALNEKYSIQGSFDWVLELDSSN 246

Query: 242 EEKFSRHLIIRVPKVAFKDNSHAGAFVGEICSRICSAKVEGRYEELFIKKDSSSTERPSH 301
           EEKFSRHLIIRVPKVAFKDNSHAGAFVGEICSRICSAKVEGRYEELFIKKDSSSTERPSH
Sbjct: 247 EEKFSRHLIIRVPKVAFKDNSHAGAFVGEICSRICSAKVEGRYEELFIKKDSSSTERPSH 306

Query: 302 LFVDNAVYSRNRCFRLALSSKAGKTSVLLPTGRHACTKMCEEDMFMASLICNVDADCEKL 361
           LFVDNAVYSRNRCFRLALSSKAGKTSVLLPTGRHACTKMCEEDMFMASLICNVDADCEKL
Sbjct: 307 LFVDNAVYSRNRCFRLALSSKAGKTSVLLPTGRHACTKMCEEDMFMASLICNVDADCEKL 366

Query: 362 LVCKMDLDCIKTLQFEMEEKCNFGQNFSFTKEAVLTGSMRDFSATSCMGISPFPDVDKFV 421
           LVCKMDLDCIKTLQFEMEEKCNFG NFSFTKEAVLTGSMRDFSATSCMG SPFPDVDKFV
Sbjct: 367 LVCKMDLDCIKTLQFEMEEKCNFGHNFSFTKEAVLTGSMRDFSATSCMGKSPFPDVDKFV 426

Query: 422 QSVASNGNVAGKIRCWYWFSEFGLIVYSMSSNRYCERIGREHKSNHVMYVVDLRRAAYYQ 481
           QSVAS GNVAGKIRCWYWFSEFGLIVYSMSSNRYCERIGREHKSNHVMYVVDLRRAAYYQ
Sbjct: 427 QSVASTGNVAGKIRCWYWFSEFGLIVYSMSSNRYCERIGREHKSNHVMYVVDLRRAAYYQ 486

Query: 482 KCHDPDCRGYRSPLRPIPIDAIPSPWISTDSGQISNDMVSTHDCIDYHQSPSNDENCLLL 541
           KCHDPDCRGYRSPLRPIPIDAIPSPWISTDSGQISNDMVSTHDCIDYHQSPSNDENCLLL
Sbjct: 487 KCHDPDCRGYRSPLRPIPIDAIPSPWISTDSGQISNDMVSTHDCIDYHQSPSNDENCLLL 546

Query: 542 FSDKNITDSSVKDSWWLEVMRVADDVENKRNMVLGKLDFEDDDWWMAVENSVKDLST 586
           FSDKNITDSSVKDSWWLEVMRVADDVENKRNMVLGKLDFEDDDWWMAVENSVKDLST
Sbjct: 547 FSDKNITDSSVKDSWWLEVMRVADDVENKRNMVLGKLDFEDDDWWMAVENSVKDLST 603

BLAST of Cucsa.108190 vs. TrEMBL
Match: V4S4L7_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10006841mg PE=4 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 1.9e-212
Identity = 377/605 (62.31%), Postives = 449/605 (74.21%), Query Frame = 1

Query: 1   MDDVDRLFECFKCGVSPPQSAVRRKKKRKSKVKPECSVNFETPVSG-----PVEETEENA 60
           MDDVDRLF+CFKCG+SPP SA+R +K+ K+++K +CS       SG           ++A
Sbjct: 1   MDDVDRLFQCFKCGISPPPSALRERKRSKNRLKQDCSKQEVASSSGLFSPGSAGNGHKSA 60

Query: 61  SSIQDTVEKF----------------SPIVFYGSPRGVPPKRPSSLWRLLREIRVDLSEQ 120
           + +  + EK                 SP+VFYG+P GVPPKRP SL RLLREIRVDLS++
Sbjct: 61  TDVHFSSEKLGSSTVKAKGSNSGKQISPVVFYGTPHGVPPKRPLSLLRLLREIRVDLSKE 120

Query: 121 SRFKLSKQVWATFPRQEEAIKFSREHTDVHIFSYQDHFNGQRRFLVSSYSEFWHRYKSMD 180
               L K++WATFPRQ EAI F++ H +V IFSYQDH+NGQRRFLVS+Y EFW RYK MD
Sbjct: 121 DSSNLGKEIWATFPRQNEAIMFAKGHANVRIFSYQDHYNGQRRFLVSTYKEFWQRYKRMD 180

Query: 181 AKCRHHYEVIQEGAPCHLYFDLEYSKRINTGKNGDEMVDSLISVVLQALNEKYSIQGSFD 240
           +K RHHYEVIQEG PCHLYFDLE++KR+N   +GDEMVD LISV L+AL +KYSI+G  D
Sbjct: 181 SKFRHHYEVIQEGLPCHLYFDLEFNKRVNPENHGDEMVDLLISVTLEALFDKYSIKGDED 240

Query: 241 WVLELDSSNEEKFSRHLIIRVPKVAFKDNSHAGAFVGEICSRICSAKV-EGRYEELFIKK 300
           W++ELDSS EEKFSRHLIIR+PK AFKDNSHAGAFV EIC+RI SA+  E ++E LF+ K
Sbjct: 241 WIVELDSSTEEKFSRHLIIRIPKTAFKDNSHAGAFVAEICARIKSAREREAKFESLFVMK 300

Query: 301 DSSSTERPSHLFVDNAVYSRNRCFRLALSSKAGKTSVLLPTGRHACTKMCEEDMFMASLI 360
           DSSSTE  S LFVD AVYSRNRCFR+ALSSKAGK S LLP+ R  C  MCEEDMFMASLI
Sbjct: 301 DSSSTESESELFVDTAVYSRNRCFRIALSSKAGKKSFLLPSERFKCKGMCEEDMFMASLI 360

Query: 361 CNVDADCEKLLVCKMDLDCIKTLQFEMEEKCNFGQNFSFTKEAVLTGSMRDFSATSCMGI 420
           CN+DADCEKLLVCKM+LDC+KTLQF+ E   N G+    ++E  L       SA S +G 
Sbjct: 361 CNMDADCEKLLVCKMELDCVKTLQFDTEVHDNLGKYCRASQECAL-------SACSSVGK 420

Query: 421 SPFPDVDKFVQSVASNGNVAGKIRCWYWFSEFGLIVYSMSSNRYCERIGREHKSNHVMYV 480
           SPFP +DKFV+ +AS GN  GKIR WYWFSE+GLIVYSMS NRYCERIGREHKSNHVMYV
Sbjct: 421 SPFPALDKFVECIASTGNDPGKIRSWYWFSEYGLIVYSMSRNRYCERIGREHKSNHVMYV 480

Query: 481 VDLRRAAYYQKCHDPDCRGYRSPLRPIPIDAIPSPWISTDSGQISNDMVSTHDCIDYHQS 540
           VDLRRA YYQKCHDPDCRGYRSPLRPIP+D IP    S+DS  + +   S+H+  +Y Q 
Sbjct: 481 VDLRRAVYYQKCHDPDCRGYRSPLRPIPMDPIPDLPFSSDSEHMVHHGGSSHNNTEY-QF 540

Query: 541 PSNDENCLLLFSDKNITDSSVKDSWWLEVMRVADDVENKR-NMVLGKL---DFEDDDWWM 580
             NDE   LL+ D+  T+S  KDSWW+E ++ A+DVENK+    LG L   D EDD+WWM
Sbjct: 541 VKNDEKHDLLYDDERDTESYCKDSWWIEAVKFANDVENKKMTCELGNLANNDDEDDNWWM 597

BLAST of Cucsa.108190 vs. TrEMBL
Match: B9RIS2_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1582000 PE=4 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 6.0e-211
Identity = 372/601 (61.90%), Postives = 452/601 (75.21%), Query Frame = 1

Query: 1   MDDVDRLFECFKCGVSPPQSAVRRKKKRKSKVKPECSVNFETPVSGPVEETE-------- 60
           MDDVDRLFECFKCG+SPPQSAVR +K+ KSK+     V+  +    P   T+        
Sbjct: 1   MDDVDRLFECFKCGISPPQSAVRERKRNKSKLNQGNEVSSTSASPSPGSATQPKKKNAID 60

Query: 61  -----ENASSIQ----DTVEKFSPIVFYGSPRGVPPKRPSSLWRLLREIRVDLSEQSRFK 120
                +N S+++       ++ SPIVFYGSP GVPPKRP SL RLLREIR+DL+E     
Sbjct: 61  PHLSADNVSTVKANNFSRRKRISPIVFYGSPHGVPPKRPISLLRLLREIRIDLAEHQESN 120

Query: 121 LSKQVWATFPRQEEAIKFSREHTDVHIFSYQDHFNGQRRFLVSSYSEFWHRYKSMDAKCR 180
           L K+VWATFPRQ EA+ F++EH +VH+FSYQDH NGQRRFLVSSY EFW RYK+MD+K R
Sbjct: 121 LRKEVWATFPRQNEALNFAKEHMNVHVFSYQDHHNGQRRFLVSSYGEFWRRYKNMDSKFR 180

Query: 181 HHYEVIQEGAPCHLYFDLEYSKRINTGKNGDEMVDSLISVVLQALNEKYSIQGSFDWVLE 240
           HHYEVIQE  PCHLYFDLE++KR N   NGDEMVD LIS++L+AL EKYSI+G+ +W++E
Sbjct: 181 HHYEVIQEDFPCHLYFDLEFNKRENAENNGDEMVDLLISLILEALLEKYSIEGNCEWIVE 240

Query: 241 LDSSNEEKFSRHLIIRVPKVAFKDNSHAGAFVGEICSRICSAK-VEGRYEELFIKKDSSS 300
           LDSS  EKFSRHLI+R+PK AFKDNSHAGAFV EICSRI S++  +GRY++LF++K SSS
Sbjct: 241 LDSSTAEKFSRHLILRIPKTAFKDNSHAGAFVSEICSRILSSREKDGRYKKLFVRKGSSS 300

Query: 301 TERPSHLFVDNAVYSRNRCFRLALSSKAGKTSVLLPTGRHACTKMCEEDMFMASLICNVD 360
            E  S  F+D AVYSRNR FRLALSSKAGK SVLLPTGR  C  MCEEDMFMASLICN+D
Sbjct: 301 -ESASQPFIDTAVYSRNRVFRLALSSKAGKNSVLLPTGRFKCKDMCEEDMFMASLICNMD 360

Query: 361 ADCEKLLVCKMDLDCIKTLQFEMEEKCNFGQNFSFTKEAVLTGSMRDFSATSCMGISPFP 420
           ADCEKLLVCKMDLDC+KTL F+ E   N+     FT +        D S T   G SPFP
Sbjct: 361 ADCEKLLVCKMDLDCVKTLHFDTE--ANYYSRRCFTTQE------NDASMTCTTGKSPFP 420

Query: 421 DVDKFVQSVASNGNVAGKIRCWYWFSEFGLIVYSMSSNRYCERIGREHKSNHVMYVVDLR 480
            +DKFV+S+A+ G+++GKIR WYWFSE+ L+VYSMS NRYCERIGR+HKSNHV+YVVDLR
Sbjct: 421 SLDKFVESIATVGSLSGKIRSWYWFSEYELMVYSMSRNRYCERIGRQHKSNHVIYVVDLR 480

Query: 481 RAAYYQKCHDPDCRGYRSPLRPIPIDAIPSPWISTDSGQISNDMVSTHDCIDYHQSPSND 540
           RA YYQKCHDPDC+GYRSPLRP+P+  IP P  S  S Q++N +   +  +DY QS +N+
Sbjct: 481 RAVYYQKCHDPDCQGYRSPLRPVPLYLIPDPSFSYASAQVANHIGLANGTLDY-QSANNN 540

Query: 541 ENCLLLFSDKNITDSSVKDSWWLEVMRVADDVEN-KRNMVLGKLD---FEDDDWWMAVEN 580
              ++LFS++   DSS KDSWWLE  +VADD+EN K+ M L  L+    EDD+WW+AVE 
Sbjct: 541 HEGIMLFSEEYDIDSSSKDSWWLEAAKVADDIENMKKTMELSTLENIGEEDDNWWVAVER 591

BLAST of Cucsa.108190 vs. TrEMBL
Match: M5XLZ6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003300mg PE=4 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 5.1e-210
Identity = 368/600 (61.33%), Postives = 445/600 (74.17%), Query Frame = 1

Query: 1   MDDVDRLFECFKCGVSPPQSAVRRKKKRKSKVKPECSVNFETPVSGPVEETEENASSIQD 60
           MDDVDRLFECFKCG+SPPQSA+R +K+ KS +K        +      E+T++ AS IQ 
Sbjct: 1   MDDVDRLFECFKCGISPPQSALRERKRSKSNLKRRSKTQEVSSPGSAAEQTQKYASEIQL 60

Query: 61  TVEK----------------FSPIVFYGSPRGVPPKRPS-SLWRLLREIRVDLSEQSRFK 120
           + EK                FSP+VFYGSP GVPPK+PS SL RLLREIRVDL+ Q R  
Sbjct: 61  SAEKSGSGTVKANNFNRAKQFSPVVFYGSPNGVPPKKPSRSLLRLLREIRVDLATQKRLS 120

Query: 121 LSKQVWATFPRQEEAIKFSREHTDVHIFSYQDHFNGQRRFLVSSYSEFWHRYKSMDAKCR 180
             K+VWATFPRQ+EA++F++ + +VH+FSYQDH++GQRRFLVS+Y +FW RY +M+ K R
Sbjct: 121 SRKEVWATFPRQDEAMQFAKGNGNVHVFSYQDHYSGQRRFLVSTYDDFWRRYNNMNPKFR 180

Query: 181 HHYEVIQEGAPCHLYFDLEYSKRINTGKNGDEMVDSLISVVLQALNEKYSIQGSFDWVLE 240
           HHYEVIQEG PCHLYFDLE++KR N G+NGDEMVD LISV+ +AL EKYSIQG+ +W+LE
Sbjct: 181 HHYEVIQEGLPCHLYFDLEFNKRDNAGRNGDEMVDVLISVIFEALLEKYSIQGNQEWILE 240

Query: 241 LDSSNEEKFSRHLIIRVPKVAFKDNSHAGAFVGEICSRICSAKV-EGRYEELFIKKDSSS 300
           LDSS E KFSRHLIIR+ K AFKDNSH GAFV EICSRI SAK  +GR+   F++KDS S
Sbjct: 241 LDSSTEAKFSRHLIIRIEKTAFKDNSHVGAFVTEICSRISSAKERDGRFGNFFVRKDSKS 300

Query: 301 TERPSHLFVDNAVYSRNRCFRLALSSKAGKTSVLLPTGRHACTKM-CEEDMFMASLICNV 360
           +   S LFVD AVY+RNRCFRLALSSKAGK SVLLPTGR     M  EE+MFMASLICN+
Sbjct: 301 SNSASQLFVDTAVYTRNRCFRLALSSKAGKNSVLLPTGRFKAKDMQSEEEMFMASLICNL 360

Query: 361 DADCEKLLVCKMDLDCIKTLQFEMEEKCNFGQNFSFTKEAVLTGSMRDFSATSCMGISPF 420
           D DCEKLLVCK DLDCIKTL F+ E   NFG+ +S+ +E  L G   D SAT  +G SPF
Sbjct: 361 DVDCEKLLVCKTDLDCIKTLHFDTEVNRNFGKCYSWPQEFALNGGTTDASATYFLGKSPF 420

Query: 421 PDVDKFVQSVASNGNVAGKIRCWYWFSEFGLIVYSMSSNRYCERIGREHKSNHVMYVVDL 480
           P +D F++SVA+ GNV+GKIR WYWFSEFG +VYSMS NRYCERIGR+HKSNHV+YV DL
Sbjct: 421 PALDAFIESVATVGNVSGKIRSWYWFSEFGHMVYSMSRNRYCERIGRQHKSNHVIYVADL 480

Query: 481 RRAAYYQKCHDPDCRGYRSPLRPIPIDAIPSPWISTDSGQISNDMVSTHDCIDYHQSPSN 540
           RRAAYYQKCHDPDCRGYRSP RPIP+D IP         ++ ++  + H+C+ Y      
Sbjct: 481 RRAAYYQKCHDPDCRGYRSPSRPIPLDIIP---------EMVHEYRNDHECVPY------ 540

Query: 541 DENCLLLFSDKNITDSSVKDSWWLEVMRVADDVENKRNMV--LGKLDFEDDDWWMAVENS 580
                    D+N+T S + DSWWLE +RVADDVENK+  +  +  +D EDD+WWMAVE +
Sbjct: 541 --------GDENMTSSCITDSWWLEAIRVADDVENKKIDISNMENIDLEDDNWWMAVEKT 577

BLAST of Cucsa.108190 vs. TrEMBL
Match: A0A061G0G4_THECC (Coiled-coil domain-containing protein 111 isoform 1 OS=Theobroma cacao GN=TCM_015263 PE=4 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 1.9e-209
Identity = 363/605 (60.00%), Postives = 447/605 (73.88%), Query Frame = 1

Query: 1   MDDVDRLFECFKCGVSPPQSAVRRKKKRKSKVKPECSVNFE-----TPVSGPVEETEENA 60
           MDDVDRLFECFKCG+SPPQSAVR +K+RKSK+  E S         TP  G  E+ +E  
Sbjct: 10  MDDVDRLFECFKCGISPPQSAVRERKRRKSKLNQENSARKVSASPCTPSLGSAEQGKETP 69

Query: 61  SSIQDTVEK----------------FSPIVFYGSPRGVPPKRPSSLWRLLREIRVDLSEQ 120
           ++ Q +V K                 SP++FYGSP G+PPKRP SL RLL EIR+DLSEQ
Sbjct: 70  NNAQLSVGKRVSTMVKVGKFSRGKQISPVIFYGSPHGIPPKRPLSLLRLLHEIRIDLSEQ 129

Query: 121 SRFKLSKQVWATFPRQEEAIKFSREHTDVHIFSYQDHFNGQRRFLVSSYSEFWHRYKSMD 180
            +  L  +VWATFPRQ+EA+KF++ H +  +FSYQDH++GQRR+L S+Y EFW RYK MD
Sbjct: 130 EKSNLRTEVWATFPRQDEAVKFAKGHANARVFSYQDHYSGQRRYLASTYEEFWKRYKIMD 189

Query: 181 AKCRHHYEVIQEGAPCHLYFDLEYSKRINTGKNGDEMVDSLISVVLQALNEKYSIQGSFD 240
           +K RHHYEVIQEG PCHLYFDLE++KR N G++GDEMVD LISV+L+AL EKYSI G+ D
Sbjct: 190 SKLRHHYEVIQEGFPCHLYFDLEFNKRDNVGRDGDEMVDLLISVILEALLEKYSINGNQD 249

Query: 241 WVLELDSSNEEKFSRHLIIRVPKVAFKDNSHAGAFVGEICSRICSAKV-EGRYEELFIKK 300
           WV+ELDSS EEKFSRHLI+R+PK AFKDNSH GAFV EICSRI SA+  + R+E L++K 
Sbjct: 250 WVVELDSSTEEKFSRHLIMRIPKTAFKDNSHVGAFVAEICSRIASARERDKRFETLYVKN 309

Query: 301 DSSSTERPSHLFVDNAVYSRNRCFRLALSSKAGKTSVLLPTGRHACTKMCEEDMFMASLI 360
            S+S E P  LFVD AVYSRNRCFRLALSSKAGK S LLPTGR  C +M E DMFMASLI
Sbjct: 310 ASTSAESPGQLFVDTAVYSRNRCFRLALSSKAGKASFLLPTGRFKCKEMGEVDMFMASLI 369

Query: 361 CNVDADCEKLLVCKMDLDCIKTLQFEMEEKCNFGQNFSFTKEAVLTGSMRDFSATSCMGI 420
           CN++ DCEK LVCKM+LDC+KTL FE E   NFG+     +E      + D S T   G 
Sbjct: 370 CNMEVDCEKFLVCKMELDCVKTLHFETEVTSNFGRYCGAPQENTY---ISDVSTTYLTGK 429

Query: 421 SPFPDVDKFVQSVASNGNVAGKIRCWYWFSEFGLIVYSMSSNRYCERIGREHKSNHVMYV 480
           SPFP +D+F++S+AS  N +GKIR WYWFSE+GLI+YSMS NRYCERIGREHKSNHVMYV
Sbjct: 430 SPFPSLDEFIESIASTRNASGKIRSWYWFSEYGLIIYSMSRNRYCERIGREHKSNHVMYV 489

Query: 481 VDLRRAAYYQKCHDPDCRGYRSPLRPIPIDAIPSPWISTDSGQISNDMVSTHDCIDYHQS 540
           VD+RRAAYYQKC+DPDC+GYRSP+RPIP+D IP      DS QI +D   T + ++Y Q 
Sbjct: 490 VDMRRAAYYQKCYDPDCKGYRSPMRPIPMDCIPDSSFFFDSRQIVDDNGLTSNDLEY-QF 549

Query: 541 PSNDENCLLLFSDKNITDSSVKDSWWLEVMRVADDVENKRNMV----LGKLDFEDDDWWM 580
            +N+   +LL+S+++  D   KDSWWLE ++VAD+++ K   +    +  +  EDDDWW+
Sbjct: 550 VNNNNGRVLLYSNESDIDDCTKDSWWLEAIKVADNIDGKPERLMFNDMENMSDEDDDWWV 609

BLAST of Cucsa.108190 vs. TAIR10
Match: AT5G52800.3 (AT5G52800.3 DNA primases)

HSP 1 Score: 664.8 bits (1714), Expect = 4.7e-191
Identity = 345/615 (56.10%), Postives = 430/615 (69.92%), Query Frame = 1

Query: 1   MDDVDRLFECFKCGVSPPQSAVRRKKKRKSKVKPE---------CSVNFETPVSGPVEET 60
           MDDVDRLF+CFKCG+SPP SA+R KK+ K  +  E           ++  + +SG   E 
Sbjct: 10  MDDVDRLFQCFKCGISPPASAMREKKRSKKMLNSEYDSPVTCKKLQLSSLSRLSGKRGER 69

Query: 61  EENASSIQ-DTVE-----------KFSPIVFYGSPRGVPPKRPSSLWRLLREIRVDLSEQ 120
            +   S+  D+V            +FSPIVFYGSP GVPPK+P SL RLLREIR+DLSE+
Sbjct: 70  NQIDGSVSVDSVSGSRDVKKSVGRQFSPIVFYGSPNGVPPKKPLSLLRLLREIRIDLSEE 129

Query: 121 SRFKLSKQVWATFPRQEEAIKFSREHTDVHIFSYQDHFNGQRRFLVSSYSEFWHRYKSMD 180
            +    K VWATFPRQEEAIKF + H +V IFSYQDHF+GQRRFLVSSY EFW RY SMD
Sbjct: 130 RKAISRKGVWATFPRQEEAIKFEKRHDNVRIFSYQDHFSGQRRFLVSSYEEFWKRYLSMD 189

Query: 181 AKCRHHYEVIQEGAPCHLYFDLEYSKRINTGKNGDEMVDSLISVVLQALNEKYSIQGSFD 240
            + RHHYEVIQEG PCH+YFDLE++++ N GKN DEMVD LISV+L+AL EKY+I+G  D
Sbjct: 190 PRHRHHYEVIQEGLPCHMYFDLEFNQKENEGKNVDEMVDILISVILEALREKYAIEGQED 249

Query: 241 WVLELDSSNEEKFSRHLIIRVPKVAFKDNSHAGAFVGEICSRICSAK-VEGRYEELFIKK 300
           W++ELDSS ++KFSRH+I+ +PKVAFKDNSH GAFVGE+CSRI +AK  + R  +LF+ K
Sbjct: 250 WIVELDSSTKDKFSRHVIVIIPKVAFKDNSHVGAFVGELCSRIVNAKETDERLRKLFVHK 309

Query: 301 DSSSTERPSHLFVDNAVYSRNRCFRLALSSKAGKTSVLLPTGRHACTKMCEEDMFMASLI 360
           +++  +  S LFVD AVYSRNRCFRLALSSKAGKTSVLLPTGR  C  M E D+FM SLI
Sbjct: 310 EAN--DSASLLFVDTAVYSRNRCFRLALSSKAGKTSVLLPTGRFKCKDMGERDVFMTSLI 369

Query: 361 CNVDADCEKLLVCKMDLDCIKTLQFEMEEKCNFGQNFSFTKEAVLTGSMRDFSATSCMGI 420
           CNV++DCEKLLVCKM+ DC+KTL F+ E   N        ++  L  S  D S +   G 
Sbjct: 370 CNVESDCEKLLVCKMESDCMKTLCFDTEVNSNNLVRDQNAQKFQLNASTSDMSTSYFGGK 429

Query: 421 SPFPDVDKFVQSVASNGNVAGKIRCWYWFSEFGLIVYSMSSNRYCERIGREHKSNHVMYV 480
           SPFP +D+F++S AS GNV GKIRCWYWFSE GLIVYSM  NRYCERIGREHKSNHVMY+
Sbjct: 430 SPFPQLDQFIESTASTGNVPGKIRCWYWFSEDGLIVYSMLRNRYCERIGREHKSNHVMYI 489

Query: 481 VDLRRAAYYQKCHDPDCRGYRSPLRPIPIDAIPSPWISTDSG--QISNDMVSTHDC-IDY 540
           VDLRR  YYQKC+DPDCR YRSP+RP+P   +P   +        + +++ S  +C +D 
Sbjct: 490 VDLRRGIYYQKCYDPDCRDYRSPIRPVPDSYLPEDMVYYQGATQNLCDNLYSEGECHVDK 549

Query: 541 HQSPSNDENCLLLFSDKNITDSSVKDSWWLEVMRVADDVENKRNMVLGKLDF----EDDD 586
              P +               ++ + SWWLE ++VADD+E+K    L  L +    EDDD
Sbjct: 550 ECDPDS---------------AASRGSWWLEAVKVADDLESKPK-TLEPLTWENYEEDDD 606

BLAST of Cucsa.108190 vs. NCBI nr
Match: gi|778679721|ref|XP_011651189.1| (PREDICTED: DNA-directed primase/polymerase protein [Cucumis sativus])

HSP 1 Score: 1189.9 bits (3077), Expect = 0.0e+00
Identity = 579/597 (96.98%), Postives = 580/597 (97.15%), Query Frame = 1

Query: 2   DDVDRLFECFKCGVSPPQSAVRRKKKRKSKVKPECSVNFETPVSGPVEETEENASSIQDT 61
           DDVDRLFECFKCGVSPPQSAVRRKKKRKSKVKPECSVNFETPVSGPVE+ EENASSIQDT
Sbjct: 7   DDVDRLFECFKCGVSPPQSAVRRKKKRKSKVKPECSVNFETPVSGPVEKAEENASSIQDT 66

Query: 62  VEK-------------FSPIVFYGSPRGVPPKRPSSLWRLLREIRVDLSEQSRFKLSKQV 121
           VEK             FSPIVFYGSPRGVPPKRPSSLWRLLREIRVDLSEQSRFKLSKQV
Sbjct: 67  VEKIGLTSKDKGSRSKFSPIVFYGSPRGVPPKRPSSLWRLLREIRVDLSEQSRFKLSKQV 126

Query: 122 WATFPRQEEAIKFSREHTDVHIFSYQDHFNGQRRFLVSSYSEFWHRYKSMDAKCRHHYEV 181
           WATFPRQEEAIKFSREHTDVHIFSYQDHFNGQRRFLVSSYSEFWHRYKSMDAKCRHHYEV
Sbjct: 127 WATFPRQEEAIKFSREHTDVHIFSYQDHFNGQRRFLVSSYSEFWHRYKSMDAKCRHHYEV 186

Query: 182 IQEGAPCHLYFDLEYSKRINTGKNGDEMVDSLISVVLQALNEKYSIQGSFDWVLELDSSN 241
           IQEGAPCHLYFDLEYSKRINTGKNGDEMVDSLISVVLQALNEKYSIQGSFDWVLELDSSN
Sbjct: 187 IQEGAPCHLYFDLEYSKRINTGKNGDEMVDSLISVVLQALNEKYSIQGSFDWVLELDSSN 246

Query: 242 EEKFSRHLIIRVPKVAFKDNSHAGAFVGEICSRICSAKVEGRYEELFIKKDSSSTERPSH 301
           EEKFSRHLIIRVPKVAFKDNSHAGAFVGEICSRICSAKVEGRYEELFIKKDSSSTERPSH
Sbjct: 247 EEKFSRHLIIRVPKVAFKDNSHAGAFVGEICSRICSAKVEGRYEELFIKKDSSSTERPSH 306

Query: 302 LFVDNAVYSRNRCFRLALSSKAGKTSVLLPTGRHACTKMCEEDMFMASLICNVDADCEKL 361
           LFVDNAVYSRNRCFRLALSSKAGKTSVLLPTGRHACTKMCEEDMFMASLICNVDADCEKL
Sbjct: 307 LFVDNAVYSRNRCFRLALSSKAGKTSVLLPTGRHACTKMCEEDMFMASLICNVDADCEKL 366

Query: 362 LVCKMDLDCIKTLQFEMEEKCNFGQNFSFTKEAVLTGSMRDFSATSCMGISPFPDVDKFV 421
           LVCKMDLDCIKTLQFEMEEKCNFG NFSFTKEAVLTGSMRDFSATSCMG SPFPDVDKFV
Sbjct: 367 LVCKMDLDCIKTLQFEMEEKCNFGHNFSFTKEAVLTGSMRDFSATSCMGKSPFPDVDKFV 426

Query: 422 QSVASNGNVAGKIRCWYWFSEFGLIVYSMSSNRYCERIGREHKSNHVMYVVDLRRAAYYQ 481
           QSVAS GNVAGKIRCWYWFSEFGLIVYSMSSNRYCERIGREHKSNHVMYVVDLRRAAYYQ
Sbjct: 427 QSVASTGNVAGKIRCWYWFSEFGLIVYSMSSNRYCERIGREHKSNHVMYVVDLRRAAYYQ 486

Query: 482 KCHDPDCRGYRSPLRPIPIDAIPSPWISTDSGQISNDMVSTHDCIDYHQSPSNDENCLLL 541
           KCHDPDCRGYRSPLRPIPIDAIPSPWISTDSGQISNDMVSTHDCIDYHQSPSNDENCLLL
Sbjct: 487 KCHDPDCRGYRSPLRPIPIDAIPSPWISTDSGQISNDMVSTHDCIDYHQSPSNDENCLLL 546

Query: 542 FSDKNITDSSVKDSWWLEVMRVADDVENKRNMVLGKLDFEDDDWWMAVENSVKDLST 586
           FSDKNITDSSVKDSWWLEVMRVADDVENKRNMVLGKLDFEDDDWWMAVENSVKDLST
Sbjct: 547 FSDKNITDSSVKDSWWLEVMRVADDVENKRNMVLGKLDFEDDDWWMAVENSVKDLST 603

BLAST of Cucsa.108190 vs. NCBI nr
Match: gi|659077640|ref|XP_008439308.1| (PREDICTED: DNA-directed primase/polymerase protein [Cucumis melo])

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 553/597 (92.63%), Postives = 567/597 (94.97%), Query Frame = 1

Query: 2   DDVDRLFECFKCGVSPPQSAVRRKKKRKSKVKPECSVNFETPVSGPVEETEENASSIQDT 61
           DDVDRLFECFKCGVSPPQSAVRR+KKRKSK+KPECSVN ETPVSGPVEE EENASS+QDT
Sbjct: 7   DDVDRLFECFKCGVSPPQSAVRREKKRKSKMKPECSVNSETPVSGPVEEREENASSVQDT 66

Query: 62  VEK-------------FSPIVFYGSPRGVPPKRPSSLWRLLREIRVDLSEQSRFKLSKQV 121
           VEK             FSPI+FYGSPRGVPPKRPSSLWRLLREIRVDLSEQSRFKLSKQV
Sbjct: 67  VEKIGLTSKDKGSRSKFSPIIFYGSPRGVPPKRPSSLWRLLREIRVDLSEQSRFKLSKQV 126

Query: 122 WATFPRQEEAIKFSREHTDVHIFSYQDHFNGQRRFLVSSYSEFWHRYKSMDAKCRHHYEV 181
           WATFPRQEEAIKF+REH DVHIFSYQDHFNGQRRFLVSSYSEFWHRYKSMDAKCRHHYEV
Sbjct: 127 WATFPRQEEAIKFAREHIDVHIFSYQDHFNGQRRFLVSSYSEFWHRYKSMDAKCRHHYEV 186

Query: 182 IQEGAPCHLYFDLEYSKRINTGKNGDEMVDSLISVVLQALNEKYSIQGSFDWVLELDSSN 241
           IQEGAPCHLYFDLEYSKRIN GKNGDEMVDSLISVVLQALNEKYSIQGSFDWVLELDSSN
Sbjct: 187 IQEGAPCHLYFDLEYSKRINMGKNGDEMVDSLISVVLQALNEKYSIQGSFDWVLELDSSN 246

Query: 242 EEKFSRHLIIRVPKVAFKDNSHAGAFVGEICSRICSAKVEGRYEELFIKKDSSSTERPSH 301
           EEKFSRHLIIR+PKVAFKDNSHAGAFVGEICSRIC AKVEG+YEELFIKKDSSSTE PSH
Sbjct: 247 EEKFSRHLIIRIPKVAFKDNSHAGAFVGEICSRICCAKVEGKYEELFIKKDSSSTESPSH 306

Query: 302 LFVDNAVYSRNRCFRLALSSKAGKTSVLLPTGRHACTKMCEEDMFMASLICNVDADCEKL 361
           LFVDNAVYSRNRCFRLALSSKAGKTSVLLP+GRHA TKMCEEDMFMASLICNVDA+CEKL
Sbjct: 307 LFVDNAVYSRNRCFRLALSSKAGKTSVLLPSGRHAYTKMCEEDMFMASLICNVDAECEKL 366

Query: 362 LVCKMDLDCIKTLQFEMEEKCNFGQNFSFTKEAVLTGSMRDFSATSCMGISPFPDVDKFV 421
           LVCKMDLDCIKTLQFE EEKCNFG+N SFT+EAVLTGSMRD SATSCMG SPFPDVDKFV
Sbjct: 367 LVCKMDLDCIKTLQFETEEKCNFGRNVSFTQEAVLTGSMRDISATSCMGKSPFPDVDKFV 426

Query: 422 QSVASNGNVAGKIRCWYWFSEFGLIVYSMSSNRYCERIGREHKSNHVMYVVDLRRAAYYQ 481
           QSVAS G+VAGKIRCWYWFSE+GLIVYSMS NRYCERIGREHKSNHVMYVVDLR AAYYQ
Sbjct: 427 QSVASTGSVAGKIRCWYWFSEYGLIVYSMSRNRYCERIGREHKSNHVMYVVDLRSAAYYQ 486

Query: 482 KCHDPDCRGYRSPLRPIPIDAIPSPWISTDSGQISNDMVSTHDCIDYHQSPSNDENCLLL 541
           KCHDPDCRGYRSPLRPIPIDAIPSPW+STDSGQISNDMVST DCIDY Q PSNDENCLLL
Sbjct: 487 KCHDPDCRGYRSPLRPIPIDAIPSPWVSTDSGQISNDMVSTDDCIDY-QFPSNDENCLLL 546

Query: 542 FSDKNITDSSVKDSWWLEVMRVADDVENKRNMVLGKLDFEDDDWWMAVENSVKDLST 586
           FSDKNITDSSVKDSWWLEVMRVADDVENKRNMVLGKLDFEDDDWWMAVENSVKDLST
Sbjct: 547 FSDKNITDSSVKDSWWLEVMRVADDVENKRNMVLGKLDFEDDDWWMAVENSVKDLST 602

BLAST of Cucsa.108190 vs. NCBI nr
Match: gi|802768759|ref|XP_012090174.1| (PREDICTED: DNA-directed primase/polymerase protein [Jatropha curcas])

HSP 1 Score: 760.8 bits (1963), Expect = 1.8e-216
Identity = 381/604 (63.08%), Postives = 458/604 (75.83%), Query Frame = 1

Query: 1   MDDVDRLFECFKCGVSPPQSAVRRKKKRKSKVKPECSVNFETPVSGP------VEETEEN 60
           MDDVDRLFECFKCGVSPPQSAVR +K+ KSK+K    ++  +  SG        +  ++N
Sbjct: 6   MDDVDRLFECFKCGVSPPQSAVRERKRNKSKLKQGNGIHEVSSASGTPSPGSATQPKKKN 65

Query: 61  ASSIQDTVE----------------KFSPIVFYGSPRGVPPKRPSSLWRLLREIRVDLSE 120
           A+  Q + E                +FSPIVFYGSP GVPPKRP SL RLL EIR+DL+E
Sbjct: 66  ATDAQVSKEELGLTVVGEKNFSHGRQFSPIVFYGSPHGVPPKRPISLLRLLHEIRIDLAE 125

Query: 121 QSRFKLSKQVWATFPRQEEAIKFSREHTDVHIFSYQDHFNGQRRFLVSSYSEFWHRYKSM 180
           Q +  L K+VWATFPRQ EA+ F++EH +VH+FSYQDH NGQRRFLVS+Y EFW RYK+M
Sbjct: 126 QQKSDLRKEVWATFPRQNEALNFAKEHANVHVFSYQDHHNGQRRFLVSTYKEFWSRYKNM 185

Query: 181 DAKCRHHYEVIQEGAPCHLYFDLEYSKRINTGKNGDEMVDSLISVVLQALNEKYSIQGSF 240
           D+K RHHYEVIQEG PCHLYFDLE+ KR N  KNGDEMVD LISV+L+A  EKYSIQG+ 
Sbjct: 186 DSKFRHHYEVIQEGVPCHLYFDLEFDKRENAEKNGDEMVDLLISVILEAFLEKYSIQGNH 245

Query: 241 DWVLELDSSNEEKFSRHLIIRVPKVAFKDNSHAGAFVGEICSRICSAKV-EGRYEELFIK 300
           +W++ELDSS  EKFSRHLIIR+PK  FKDNSHAGAFV EICSRI SA+  +GR+E+LFI+
Sbjct: 246 EWIVELDSSTAEKFSRHLIIRIPKAGFKDNSHAGAFVSEICSRILSARERDGRFEKLFIR 305

Query: 301 KDSSSTERPSHLFVDNAVYSRNRCFRLALSSKAGKTSVLLPTGRHACTKMCEEDMFMASL 360
           KDSS+ E    +F+D AVYSRNR FR+ALSSKAGK+++LLPTGR  C  MCEEDMFMASL
Sbjct: 306 KDSSA-ESTMQIFIDTAVYSRNRVFRVALSSKAGKSAILLPTGRFKCKDMCEEDMFMASL 365

Query: 361 ICNVDADCEKLLVCKMDLDCIKTLQFEMEEKCNFGQNFSFTKEAVLTGSMRDFSATSCMG 420
           ICN+D DCEKLLVCKMDLDC+KTL F+ EE C + + +          S  + S T  MG
Sbjct: 366 ICNMDTDCEKLLVCKMDLDCVKTLHFDTEENC-YSRRYD--------TSQVNLSTTYTMG 425

Query: 421 ISPFPDVDKFVQSVASNGNVAGKIRCWYWFSEFGLIVYSMSSNRYCERIGREHKSNHVMY 480
            SPFP +D FV+S+AS G+++GKIR WYWFSE+GLIVYSMS NRYCERIGR+HKSNHV+Y
Sbjct: 426 KSPFPFLDNFVESIASAGSLSGKIRSWYWFSEYGLIVYSMSRNRYCERIGRQHKSNHVIY 485

Query: 481 VVDLRRAAYYQKCHDPDCRGYRSPLRPIPIDAIPSPWISTDSGQISNDMVSTHDCIDYHQ 540
           V+DLRRA YYQKCHDPDC+GYRSPLRPIPI+ IP P    DS QI+N M  T D  DY  
Sbjct: 486 VIDLRRAVYYQKCHDPDCQGYRSPLRPIPINLIPDPSFVHDSVQIANHMGLTSDTPDYQS 545

Query: 541 SPSNDENCLLLFSDKNITDSSVKDSWWLEVMRVADDVENKRNMV-LGKLDF-EDDDWWMA 580
             S  E  +LL++D++ TDS  KDSWWLE  +VADD+E+K+ +V L  L+  EDD+WWMA
Sbjct: 546 VNSGCEG-ILLYNDEHDTDSCAKDSWWLEAAKVADDIESKQKLVELSTLNVNEDDNWWMA 598

BLAST of Cucsa.108190 vs. NCBI nr
Match: gi|302143772|emb|CBI22633.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 760.0 bits (1961), Expect = 3.1e-216
Identity = 373/594 (62.79%), Postives = 459/594 (77.27%), Query Frame = 1

Query: 1   MDDVDRLFECFKCGVSPPQSAVRRKKKRKSKVKPECSVNFETPVS-----GPVEETEENA 60
           MDDVDRLF+CFKCGVSPP+SA+R +K+ K K+K   S +  +  S     G   E  +NA
Sbjct: 1   MDDVDRLFQCFKCGVSPPKSAIRERKRNKEKLKQAGSTHVASTYSDVQSPGSAGERHQNA 60

Query: 61  SSIQDTVEK-----FSPIVFYGSPRGVPPKRPSSLWRLLREIRVDLSEQSRFKLSKQVWA 120
           +++Q  +++      SP++FYGSP GVPPKRPSSL RLLREIRV+LSEQ++  L ++VWA
Sbjct: 61  NNVQPCMKERFNCHISPVIFYGSPHGVPPKRPSSLLRLLREIRVELSEQNKSDLREEVWA 120

Query: 121 TFPRQEEAIKFSREHTDVHIFSYQDHFNGQRRFLVSSYSEFWHRYKSMDAKCRHHYEVIQ 180
           TFPRQ+E+IKF++ H DVHIFSYQDH NGQRRFLVS+Y EFW RYK+MD+K RHHYEVIQ
Sbjct: 121 TFPRQDESIKFAQGHADVHIFSYQDHLNGQRRFLVSTYKEFWQRYKNMDSKFRHHYEVIQ 180

Query: 181 EGAPCHLYFDLEYSKRINTGKNGDEMVDSLISVVLQALNEKYSIQGSFDWVLELDSSNEE 240
           EG PCHLYFDLE++K+ N  K+GDEMVD LISVV +AL EKYSIQ +  W++ELDSS  E
Sbjct: 181 EGFPCHLYFDLEFNKKDNAEKDGDEMVDLLISVVSEALLEKYSIQVNEKWIVELDSSTGE 240

Query: 241 KFSRHLIIRVPKVAFKDNSHAGAFVGEICSRICSA-KVEGRYEELFIKKDSSSTERPSHL 300
           KFSRHLIIR+PK+AFKDNSHAG FV EICS+I SA + +G +++LFI K S+S E P  L
Sbjct: 241 KFSRHLIIRIPKIAFKDNSHAGVFVAEICSQISSAGEGDGNFKKLFISKGSTSAEFPCQL 300

Query: 301 FVDNAVYSRNRCFRLALSSKAGKTSVLLPTGRHACTKMCEEDMFMASLICNVDADCEKLL 360
           FVD AVYSRNRCFRLALSSKAGK SVLLPTGR  C  MCEE+MFMASLICN+D DCEK L
Sbjct: 301 FVDTAVYSRNRCFRLALSSKAGKNSVLLPTGRFKCKDMCEEEMFMASLICNIDVDCEKFL 360

Query: 361 VCKMDLDCIKTLQFEMEEKCNFGQNFSFTKEAVLTGSMRDFSATSCMGISPFPDVDKFVQ 420
           VCKMDL+C+KTL F+MEE  N  ++ S  ++  L+    + S T  MG SPFP +D FV+
Sbjct: 361 VCKMDLNCVKTLHFDMEENHNCRKHSSSPQDFALSACTSNVSGTCFMGNSPFPALDIFVE 420

Query: 421 SVASNGNVAGKIRCWYWFSEFGLIVYSMSSNRYCERIGREHKSNHVMYVVDLRRAAYYQK 480
           S+AS GNV+GKIR WYWFSE+ L+VYSMS NRYCERIGR+HKSNHV+YVVDLRRA +YQK
Sbjct: 421 SIASIGNVSGKIRSWYWFSEYRLMVYSMSRNRYCERIGRQHKSNHVIYVVDLRRAIFYQK 480

Query: 481 CHDPDCRGYRSPLRPIPIDAIPSPWISTDSGQISNDMVSTHDCIDYHQSPSNDENCLLLF 540
           CHDPDCRGYRSP RPIP+D IP+  +  DS Q  + + ST + + Y     ND+ C+ L+
Sbjct: 481 CHDPDCRGYRSPSRPIPMDVIPNTAVLMDSVQTEHHVESTSNHLGY-SLVENDQECVSLY 540

Query: 541 SDKNITDSSVKDSWWLEVMRVADDVENKRNM----VLGKLDFEDDDWWMAVENS 580
           +DK+ITDS  KD+WWLE +R AD+VE+ ++M        +D EDDDWWMA E +
Sbjct: 541 NDKSITDSCKKDAWWLEAIRFADNVESVQHMRDLSDTENIDDEDDDWWMAAERT 593

BLAST of Cucsa.108190 vs. NCBI nr
Match: gi|731423921|ref|XP_010662672.1| (PREDICTED: DNA-directed primase/polymerase protein isoform X1 [Vitis vinifera])

HSP 1 Score: 756.5 bits (1952), Expect = 3.4e-215
Identity = 373/603 (61.86%), Postives = 459/603 (76.12%), Query Frame = 1

Query: 1   MDDVDRLFECFKCGVSPPQSAVRRKKKRKSKVKPECSVNFETPVS-----GPVEETEENA 60
           MDDVDRLF+CFKCGVSPP+SA+R +K+ K K+K   S +  +  S     G   E  +NA
Sbjct: 1   MDDVDRLFQCFKCGVSPPKSAIRERKRNKEKLKQAGSTHVASTYSDVQSPGSAGERHQNA 60

Query: 61  SSIQDTVEKF--------------SPIVFYGSPRGVPPKRPSSLWRLLREIRVDLSEQSR 120
           +++Q  +++               SP++FYGSP GVPPKRPSSL RLLREIRV+LSEQ++
Sbjct: 61  NNVQPCMKELGPRTVEAERFNCHISPVIFYGSPHGVPPKRPSSLLRLLREIRVELSEQNK 120

Query: 121 FKLSKQVWATFPRQEEAIKFSREHTDVHIFSYQDHFNGQRRFLVSSYSEFWHRYKSMDAK 180
             L ++VWATFPRQ+E+IKF++ H DVHIFSYQDH NGQRRFLVS+Y EFW RYK+MD+K
Sbjct: 121 SDLREEVWATFPRQDESIKFAQGHADVHIFSYQDHLNGQRRFLVSTYKEFWQRYKNMDSK 180

Query: 181 CRHHYEVIQEGAPCHLYFDLEYSKRINTGKNGDEMVDSLISVVLQALNEKYSIQGSFDWV 240
            RHHYEVIQEG PCHLYFDLE++K+ N  K+GDEMVD LISVV +AL EKYSIQ +  W+
Sbjct: 181 FRHHYEVIQEGFPCHLYFDLEFNKKDNAEKDGDEMVDLLISVVSEALLEKYSIQVNEKWI 240

Query: 241 LELDSSNEEKFSRHLIIRVPKVAFKDNSHAGAFVGEICSRICSA-KVEGRYEELFIKKDS 300
           +ELDSS  EKFSRHLIIR+PK+AFKDNSHAG FV EICS+I SA + +G +++LFI K S
Sbjct: 241 VELDSSTGEKFSRHLIIRIPKIAFKDNSHAGVFVAEICSQISSAGEGDGNFKKLFISKGS 300

Query: 301 SSTERPSHLFVDNAVYSRNRCFRLALSSKAGKTSVLLPTGRHACTKMCEEDMFMASLICN 360
           +S E P  LFVD AVYSRNRCFRLALSSKAGK SVLLPTGR  C  MCEE+MFMASLICN
Sbjct: 301 TSAEFPCQLFVDTAVYSRNRCFRLALSSKAGKNSVLLPTGRFKCKDMCEEEMFMASLICN 360

Query: 361 VDADCEKLLVCKMDLDCIKTLQFEMEEKCNFGQNFSFTKEAVLTGSMRDFSATSCMGISP 420
           +D DCEK LVCKMDL+C+KTL F+MEE  N  ++ S  ++  L+    + S T  MG SP
Sbjct: 361 IDVDCEKFLVCKMDLNCVKTLHFDMEENHNCRKHSSSPQDFALSACTSNVSGTCFMGNSP 420

Query: 421 FPDVDKFVQSVASNGNVAGKIRCWYWFSEFGLIVYSMSSNRYCERIGREHKSNHVMYVVD 480
           FP +D FV+S+AS GNV+GKIR WYWFSE+ L+VYSMS NRYCERIGR+HKSNHV+YVVD
Sbjct: 421 FPALDIFVESIASIGNVSGKIRSWYWFSEYRLMVYSMSRNRYCERIGRQHKSNHVIYVVD 480

Query: 481 LRRAAYYQKCHDPDCRGYRSPLRPIPIDAIPSPWISTDSGQISNDMVSTHDCIDYHQSPS 540
           LRRA +YQKCHDPDCRGYRSP RPIP+D IP+  +  DS Q  + + ST + + Y     
Sbjct: 481 LRRAIFYQKCHDPDCRGYRSPSRPIPMDVIPNTAVLMDSVQTEHHVESTSNHLGY-SLVE 540

Query: 541 NDENCLLLFSDKNITDSSVKDSWWLEVMRVADDVENKRNM----VLGKLDFEDDDWWMAV 580
           ND+ C+ L++DK+ITDS  KD+WWLE +R AD+VE+ ++M        +D EDDDWWMA 
Sbjct: 541 NDQECVSLYNDKSITDSCKKDAWWLEAIRFADNVESVQHMRDLSDTENIDDEDDDWWMAA 600