Cucsa.108110 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.108110
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Beta-carotene hydroxylase
Location	scaffold00931 : 327142 .. 329852 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAACTTGTGGTCTCTGTTTCTTCAAACTCCATTTTTAAACCCCTCTCCCCGCCGTCTCTTTCTCCGAGAACCTTCCTCTTCTTCCACCGTCTTTTCACTTTTCACCCCTCTTCTCTCCCATGGCGGCCGGCCTCTCCGCCGCCACTCTCCCCAAACCATTCCATCTCTTCCTTACTTCCTCCCATCTCTCCCCTAAACCTCCATCTCCTTTTCTTTTTCCACCTTCTGTCTTCCGGAACAGTAGATTCCAATGGAAGATGCGCAGAAAAACTTTACTCACTGTCTGCGTACTCGTTGAGGGTCAAAATAGTTCCGGCAAGGTAGACAATCTCTCCGATGAAGAATCGCCGATTGTAGTTCCTCAGATCCCATCGCCTCACGTTTCCGAAAGATTAGCTAGGAAGAAATCGGAGCGTTTCACTTATCTTGTTGCTGCGGTTATGTCTACTTTCGGAATTACATCCATGGCTGTCATGGCGGTTTATTACCGATTTTACTGGCAAATGGAGGTCCTCTCTTCTTCTTCTTCCTCTATCTACCTAGAAACATTAATTGCTTTGTTTTTTCTCGTTGGCGCCGCCTTCTTCTGATTGAATATTGAATTGCGGTTGTTTGATTTTGGCGGTGTGTAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCTGTTGGTGCCGCTGTACGTTTTCAATTTCCATTCATCTCTCTACTTTTCTTGATGAATTCCATATGCTTTAATCTGATTCTTCACAATCAATTTGCGATTAATCAGGTCGGCATGGAGTTCTGGGCAAGATGGGCTCATAGAGCTCTTTGGCACTCTTCCTTATGGCATATGCACGAGGTTTCTAATCCTTCTTCACTATATAATTTCTTGATTTCTGAGCAAATTTTTGTATACATTAATCAGCTCTGAGGCTTAATTTCATACCAAACAAACAATCAAAAACCTCTTCTCTATAGATTTAACTATCGATCTCTCATGTTCCAAATAATTATTTTTACAATAAAACAGAGAAAATGGTTGAGGGGCTTTGACCATTATTGATGTTGAATGTTTTGATTATTACAACAGTCGCATCATAAACCAAGAGAAGGACCATTCGAACTGAACGATATTTTCGCCATAATCAACGCTGTGCCGGCCATAGCTCTTCTTTCATACGGGTTCTTCCATAAAGGGCTTGTTCCTGGACTCTGCTTTGGCGCAGTAAATCTCTGAATTTCTCTCTCATTATCTTTGGAGAATCTCAGTTTGTTCATACTCCCAACTGTTTAATGTTTGTTTAATCACTGGGGATTAGTGCTAATAAGAGTGTTATAATGACGAAAATAGCCTTTCGAAAAGAAAGAAAAAAAGTTGTGGTATTGAGAAAGGGGTTTGACACGCTGGCGTGTTGTGGCAGGGTCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTAGGTCCCATCGCCAACGTCCCTTACTTCAGAAAGGTTGCTGCTGCTCACCAGGTAAACTTTTCTTCTCTTTTCCTATTTACCTTCACCCACTTGTTCTTAATTTTTTCCAATTATAGTCCATTTACGTATTTTTAAGTTCATTATAATCCTTAATTACTTGCTATACTTGCTACTTATTCTACATAATAAGTCTATTCTAGTTCGGATTAAATAGAAAAAGAAAATACATTATTTATAAATTTAAATCTTAATTTTGATACTGTGTTGACTTTCATTAATGACTTTTTTGTATATGGATCACTAGATGTAGATTCTACTAATTAACTATATGAAAAATGGGTTAAATAAAATCTTCTAGATGTCCTTATTTCTAAATTTAAGAATACCGAATAAGGTAAAAATATTATGATGAACTAAACATATAAATGTAGACTCAATAAATATGGCTTTAAATCAACCAACTATTAGATACCAATAATAGCTCAAATAATAAGATAGCCATGGAGTCTGAAAAGTTCTAGATGTGACGTTAGTATTTTTTTTTTTTTACAGTTAAAACTACTTTTACATTTTACCCGTATCTTAGAGTAAATCTAAATTTAAAATTGAAGCTTATGATTTTTCTTTTCCCCTTTTTCTCCTTTGCTTTAGCTTCACCATTCGGACAAGTTCAATGGTGTGCCATACGGGCTTTTTTTGGGTCCTAAGGTATGTTTCAGTTCATATATTCTTTAATTTTATTTAAAAGGGTTATTTACAAAAACATAACAAAGTTTCATATAACTTTCATATTTCATAGTGTATCAATAATAATCCTATTATTCTATTGGGTCTATCCGTAGTAAATATTAAATTTTGTAATATTTTGTAAATATTTAGATTGATTTTGTTATATTTAAAATTGTATTTTTTTTAAAAAAAATTAAATGGTGGGTGTGCAGGAATTAGAGGAAGTGGGAGGGTTAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATGGACGGCTCGAAAATCAATAGATGGTTCTTGATCAACCAAATATTATGTGAAAATTAGAGAGGAAAAAAAAAAGAAGCTTTACTTGAGCAGTAACTTTTTTCTTTTTCTAATATTAAAAAGTAAAATATTATTTAGTTCAGAGCTTTTTCTTTTTCTATTATGTACGAAGTAAAGGAGTAAAAGAATGAGATAGTGAATTGGGTACACAATT

mRNA sequence

AAAAAACTTGTGGTCTCTGTTTCTTCAAACTCCATTTTTAAACCCCTCTCCCCGCCGTCTCTTTCTCCGAGAACCTTCCTCTTCTTCCACCGTCTTTTCACTTTTCACCCCTCTTCTCTCCCATGGCGGCCGGCCTCTCCGCCGCCACTCTCCCCAAACCATTCCATCTCTTCCTTACTTCCTCCCATCTCTCCCCTAAACCTCCATCTCCTTTTCTTTTTCCACCTTCTGTCTTCCGGAACAGTAGATTCCAATGGAAGATGCGCAGAAAAACTTTACTCACTGTCTGCGTACTCGTTGAGGGTCAAAATAGTTCCGGCAAGGTAGACAATCTCTCCGATGAAGAATCGCCGATTGTAGTTCCTCAGATCCCATCGCCTCACGTTTCCGAAAGATTAGCTAGGAAGAAATCGGAGCGTTTCACTTATCTTGTTGCTGCGGTTATGTCTACTTTCGGAATTACATCCATGGCTGTCATGGCGGTTTATTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCTGTTGGTGCCGCTGTCGGCATGGAGTTCTGGGCAAGATGGGCTCATAGAGCTCTTTGGCACTCTTCCTTATGGCATATGCACGAGTCGCATCATAAACCAAGAGAAGGACCATTCGAACTGAACGATATTTTCGCCATAATCAACGCTGTGCCGGCCATAGCTCTTCTTTCATACGGGTTCTTCCATAAAGGGCTTGTTCCTGGACTCTGCTTTGGCGCAGGTCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTAGGTCCCATCGCCAACGTCCCTTACTTCAGAAAGGTTGCTGCTGCTCACCAGCTTCACCATTCGGACAAGTTCAATGGTGTGCCATACGGGCTTTTTTTGGGTCCTAAGGAATTAGAGGAAGTGGGAGGGTTAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATGGACGGCTCGAAAATCAATAGATGGTTCTTGATCAACCAAATATTATGTGAAAATTAGAGAGGAAAAAAAAAAGAAGCTTTACTTGAGCAGTAACTTTTTTCTTTTTCTAATATTAAAAAGTAAAATATTATTTAGTTCAGAGCTTTTTCTTTTTCTATTATGTACGAAGTAAAGGAGTAAAAGAATGAGATAGTGAATTGGGTACACAATT

Coding sequence (CDS)

ATGGCGGCCGGCCTCTCCGCCGCCACTCTCCCCAAACCATTCCATCTCTTCCTTACTTCCTCCCATCTCTCCCCTAAACCTCCATCTCCTTTTCTTTTTCCACCTTCTGTCTTCCGGAACAGTAGATTCCAATGGAAGATGCGCAGAAAAACTTTACTCACTGTCTGCGTACTCGTTGAGGGTCAAAATAGTTCCGGCAAGGTAGACAATCTCTCCGATGAAGAATCGCCGATTGTAGTTCCTCAGATCCCATCGCCTCACGTTTCCGAAAGATTAGCTAGGAAGAAATCGGAGCGTTTCACTTATCTTGTTGCTGCGGTTATGTCTACTTTCGGAATTACATCCATGGCTGTCATGGCGGTTTATTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCTGTTGGTGCCGCTGTCGGCATGGAGTTCTGGGCAAGATGGGCTCATAGAGCTCTTTGGCACTCTTCCTTATGGCATATGCACGAGTCGCATCATAAACCAAGAGAAGGACCATTCGAACTGAACGATATTTTCGCCATAATCAACGCTGTGCCGGCCATAGCTCTTCTTTCATACGGGTTCTTCCATAAAGGGCTTGTTCCTGGACTCTGCTTTGGCGCAGGTCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTAGGTCCCATCGCCAACGTCCCTTACTTCAGAAAGGTTGCTGCTGCTCACCAGCTTCACCATTCGGACAAGTTCAATGGTGTGCCATACGGGCTTTTTTTGGGTCCTAAGGAATTAGAGGAAGTGGGAGGGTTAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATGGACGGCTCGAAAATCAATAGATGGTTCTTGA

Protein sequence

MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVEGQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS*

Homology

[Close]

BLAST of Cucsa.108110 vs. Swiss-Prot
Match: BCH2_CAPAN (Beta-carotene hydroxylase 2, chloroplastic (Fragment) OS=Capsicum annuum GN=CA2 PE=2 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 1.5e-117
Identity = 209/309 (67.64%), Postives = 238/309 (77.02%), Query Frame = 1


Query: 25  PKPPSPFLFPPSVFR-NSRFQWKMRRKTLLTVCVLVEGQNSSGKVDNLSDEESPIVVPQI 84
           P P +P ++P + F  N     + RR+   TVC ++E      + +   ++    +  QI
Sbjct: 35  PTPTTPSVYPITPFSPNLGSILRCRRRPSFTVCFVLEDDKFKTQFEAGEEDIEMKIEEQI 94

Query: 85  PSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTF 144
            +  ++E+LARKKSERFTYLVAAVMS+FGITSMAVMAVYYRFYWQMEGGE+PFSEMFGTF
Sbjct: 95  SATRLAEKLARKKSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEVPFSEMFGTF 154

Query: 145 SLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAINQVGMEFWARWAHRALWHSSLWHM 204
           +LSVGAA                               VGMEFWARWAH+ALWH+SLWHM
Sbjct: 155 ALSVGAA-------------------------------VGMEFWARWAHKALWHASLWHM 214

Query: 205 HESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMF 264
           HESHHKPREGPFELND+FAIINAVPAIALL YGFFHKGL+PGLCFGAGLGITVFGMAYMF
Sbjct: 215 HESHHKPREGPFELNDVFAIINAVPAIALLDYGFFHKGLIPGLCFGAGLGITVFGMAYMF 274

Query: 265 VHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELE 324
           VHDGLVHKRFPVGP+ANVPY RKVAAAH LHHS+KFNGVPYGLFLGPKELEEVGGLEELE
Sbjct: 275 VHDGLVHKRFPVGPVANVPYLRKVAAAHSLHHSEKFNGVPYGLFLGPKELEEVGGLEELE 312

Query: 325 KEINRRIKW 333
           KE+NRR ++
Sbjct: 335 KEVNRRTRY 312

[Close]

BLAST of Cucsa.108110 vs. Swiss-Prot
Match: BCH_GENLU (Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea GN=BHY PE=2 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 2.9e-113
Identity = 212/320 (66.25%), Postives = 238/320 (74.38%), Query Frame = 1


Query: 20  SSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVEGQNSSGKVDNLSDEESPIV 79
           SS +S   P+  +FPP  F+      + R K  LTVC ++E +   GK+   SD++    
Sbjct: 30  SSPVSTSTPTLVVFPP--FKLVSKSLRTRSKPRLTVCFVLEEKELRGKLVVASDDDDGAG 89

Query: 80  VPQ--------IPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMAVYYRFYWQMEG 139
             +          +  ++++LARKKSERFTYLVAAVMS+FGITSMAV++VYYRF WQMEG
Sbjct: 90  EVRKQREKEISASAEKLAQKLARKKSERFTYLVAAVMSSFGITSMAVLSVYYRFSWQMEG 149

Query: 140 GEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAINQVGMEFWARWA 199
           GEIP SEMFGTF+LSVGAA                               VGMEFWARWA
Sbjct: 150 GEIPLSEMFGTFALSVGAA-------------------------------VGMEFWARWA 209

Query: 200 HRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAG 259
           H ALWH+SLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHKGL+PGLCFGAG
Sbjct: 210 HEALWHASLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHKGLIPGLCFGAG 269

Query: 260 LGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 319
           LGITVFGMAYMFVHDGLVHKRFPVGPIA+VPYFR+VAAAH LHHSDKFNGVPYGLFLGPK
Sbjct: 270 LGITVFGMAYMFVHDGLVHKRFPVGPIADVPYFRRVAAAHTLHHSDKFNGVPYGLFLGPK 316

Query: 320 ELEEVGGLEELEKEINRRIK 332
           ELEEVGGL+ LE EINRR K
Sbjct: 330 ELEEVGGLQVLEMEINRRTK 316

[Close]

BLAST of Cucsa.108110 vs. Swiss-Prot
Match: BCH1_ARATH (Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana GN=BETA-OHASE 1 PE=1 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 5.0e-110
Identity = 217/348 (62.36%), Postives = 246/348 (70.69%), Query Frame = 1


Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPK---PPSPFLFPPSVFRNSRFQWKMRRKTLLTVCV 60
           MAAGLS A   KP H   +SS    +   P S   F PS+ R  RF          +VC 
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSLSGFSPSL-RFKRF----------SVCY 60

Query: 61  LVEGQNSSGKVDNLSDEESPIVVPQIPSPHVSERLA----RKKSERFTYLVAAVMSTFGI 120
           +VE +  +  ++N    ES      I + +++ RLA    RKKSER TYL+AA++S+FGI
Sbjct: 61  VVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGI 120

Query: 121 TSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLIL 180
           TSMAVMAVYYRF WQMEGGEI   EMFGTF+LSVGAA                       
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAA----------------------- 180

Query: 181 HNQFAINQVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALL 240
                   VGMEFWARWAHRALWH+SLW+MHESHHKPREGPFELND+FAI+NA PAI LL
Sbjct: 181 --------VGMEFWARWAHRALWHASLWNMHESHHKPREGPFELNDVFAIVNAGPAIGLL 240

Query: 241 SYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQL 300
           SYGFF+KGLVPGLCFGAGLGITVFG+AYMFVHDGLVHKRFPVGPIA+VPY RKVAAAHQL
Sbjct: 241 SYGFFNKGLVPGLCFGAGLGITVFGIAYMFVHDGLVHKRFPVGPIADVPYLRKVAAAHQL 300

Query: 301 HHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 342
           HH+DKFNGVPYGLFLGPKELEEVGG EEL+KEI+RRIK   + S  GS
Sbjct: 301 HHTDKFNGVPYGLFLGPKELEEVGGNEELDKEISRRIKSYKKASGSGS 306

[Close]

BLAST of Cucsa.108110 vs. Swiss-Prot
Match: BCH2_ARATH (Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana GN=BETA-OHASE 2 PE=2 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 1.5e-109
Identity = 211/333 (63.36%), Postives = 241/333 (72.37%), Query Frame = 1


Query: 1   MAAGLSA-ATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLV 60
           MAAGLS  A   KP +    SS  +  P S  +FPPS+  N       RR+ +LTVC +V
Sbjct: 1   MAAGLSTIAVTLKPLN---RSSFSANHPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EGQNSSGKVDNLSDEESPIVVPQI-PSPHVSERLARKKSERFTYLVAAVMSTFGITSMAV 120
           E +  S  +D+ +  ES     +I  +  + ++  +KKSERFTYL+AAVMS+FGITSMA+
Sbjct: 61  EERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAI 120

Query: 121 MAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFA 180
           MAVYYRF WQM+GGE+   EMFGTF+LSVGAA                            
Sbjct: 121 MAVYYRFSWQMKGGEVSVLEMFGTFALSVGAA---------------------------- 180

Query: 181 INQVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFF 240
              VGMEFWARWAHRALWH SLW+MHESHHKPREG FELND+FAI NAVPAI LL YGF 
Sbjct: 181 ---VGMEFWARWAHRALWHDSLWNMHESHHKPREGAFELNDVFAITNAVPAIGLLYYGFL 240

Query: 241 HKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDK 300
           +KGLVPGLCFGAGLGIT+FGMAYMFVHDGLVHKRFPVGPIANVPY RKVAAAHQLHH+DK
Sbjct: 241 NKGLVPGLCFGAGLGITMFGMAYMFVHDGLVHKRFPVGPIANVPYLRKVAAAHQLHHTDK 294

Query: 301 FNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 332
           F GVPYGLFLGPKE+EEVGG EELEKEI+RRIK
Sbjct: 301 FKGVPYGLFLGPKEVEEVGGKEELEKEISRRIK 294

[Close]

BLAST of Cucsa.108110 vs. Swiss-Prot
Match: BCH1_CAPAN (Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum GN=CA1 PE=1 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 4.9e-105
Identity = 204/349 (58.45%), Postives = 240/349 (68.77%), Query Frame = 1


Query: 1   MAAGLSAATLPKPFHL----FLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVC 60
           MAA +S +   +   L    F    + +  PP  F  P +   N     + RRK  L  C
Sbjct: 1   MAAEISISASSRAICLQRNPFPAPKYFATAPPLLFFSPLTC--NLDAILRSRRKPRLAAC 60

Query: 61  VLVEGQN----SSGK----------VDNLSDEESPIVVPQIPSPHVSERLARKKSERFTY 120
            +++        SGK          ++  ++EE  + V       ++E+ ARKKSERFTY
Sbjct: 61  FVLKDDKLYTAQSGKQSDTEAIGDEIEVETNEEKSLAV------RLAEKFARKKSERFTY 120

Query: 121 LVAAVMSTFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLF 180
           LVAAVMS+ GITSMAV++VYYRF WQMEGGE+PFSEMF TF+L+ GAA            
Sbjct: 121 LVAAVMSSLGITSMAVISVYYRFSWQMEGGEMPFSEMFCTFALAFGAA------------ 180

Query: 181 LMNSICFNLILHNQFAINQVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFA 240
                              +GME+WARWAHRALWH+SLWHMHESHH+PREGPFELNDIFA
Sbjct: 181 -------------------IGMEYWARWAHRALWHASLWHMHESHHRPREGPFELNDIFA 240

Query: 241 IINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVP 300
           IINAVPAIA  S+GF HKGL+PG+CFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIA VP
Sbjct: 241 IINAVPAIAFFSFGFNHKGLIPGICFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIAKVP 300

Query: 301 YFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 332
           YF++VAAAHQLHHSDKF+GVPYGLFLGPKELEEVG +EELEKE+NRRIK
Sbjct: 301 YFQRVAAAHQLHHSDKFDGVPYGLFLGPKELEEVGVIEELEKEVNRRIK 310

[Close]

BLAST of Cucsa.108110 vs. TrEMBL
Match: A0A0A0L606_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G183920 PE=3 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 6.3e-176
Identity = 310/341 (90.91%), Postives = 310/341 (90.91%), Query Frame = 1


Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60
           MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120
           GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA
Sbjct: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAIN 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAA                              
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAA------------------------------ 180

Query: 181 QVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240
            VGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK
Sbjct: 181 -VGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240

Query: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300
           GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN
Sbjct: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300

Query: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 342
           GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS
Sbjct: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 310

[Close]

BLAST of Cucsa.108110 vs. TrEMBL
Match: A0A088CIY4_CUCME (Beta-carotene hydroxylase OS=Cucumis melo var. makuwa GN=CHXB PE=2 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 7.7e-166
Identity = 295/341 (86.51%), Postives = 301/341 (88.27%), Query Frame = 1


Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60
           MAA LSAA LPKPFHLFL+SSHLS KPP+PFLFPPSVF NSRFQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAALSAALLPKPFHLFLSSSHLSLKPPTPFLFPPSVFPNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120
            QNSSG+V +LSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAA+MS+FGITSMAVMA
Sbjct: 61  DQNSSGEVHSLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAIMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAIN 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAA                              
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAA------------------------------ 180

Query: 181 QVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240
            VGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK
Sbjct: 181 -VGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240

Query: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300
           GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN
Sbjct: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300

Query: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 342
           GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTA KS  GS
Sbjct: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTAPKSNYGS 310

[Close]

BLAST of Cucsa.108110 vs. TrEMBL
Match: G1FQR0_CUCMO (Chloroplast beta-carotene hydroxylase OS=Cucurbita moschata GN=CHYB PE=2 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 4.7e-155
Identity = 275/341 (80.65%), Postives = 294/341 (86.22%), Query Frame = 1


Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60
           MAA LSA   PKPFH+FLTSS+LSPKP +P LFP SVFRNSR QWKMRRKTLLTVCVLVE
Sbjct: 1   MAASLSAVLGPKPFHVFLTSSNLSPKPRTPLLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120
            Q+SS +V+NL+DE+  I+VPQIPSPHV+E+LARKKSERFTYLVAAVMS+FGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLLIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAIN 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAA                              
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAA------------------------------ 180

Query: 181 QVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240
            VGMEFWARWAHRALWH+SLWHMHESHHKPREGPFELND+FAI NAVPAIALLSYGFFHK
Sbjct: 181 -VGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAITNAVPAIALLSYGFFHK 240

Query: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300
           GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHS+K N
Sbjct: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSEKLN 300

Query: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 342
           GVPYGLFLGPKELEEVGGLEELEKEINRRIK TA+K I+GS
Sbjct: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKSTAKKPINGS 310

[Close]

BLAST of Cucsa.108110 vs. TrEMBL
Match: A0A061G159_THECC (Beta-hydroxylase 1, 1,B1,chy1,BCH1 isoform 1 OS=Theobroma cacao GN=TCM_015247 PE=3 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 4.7e-123
Identity = 236/338 (69.82%), Postives = 261/338 (77.22%), Query Frame = 1


Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60
           MAAGLSAA  PKPF  FL SSHL PKP +P   P  + R  +  +  RRK    VC ++E
Sbjct: 1   MAAGLSAAITPKPFRSFL-SSHLEPKP-TPLFHP--LLRLQKTLYGARRKRSFAVCFVLE 60

Query: 61  GQNSSG-KVDNLSDE------ESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGI 120
            Q  S  ++ NL++E      +S I++       ++E+LARK+SERFTYLVAAVMS+FGI
Sbjct: 61  EQKQSAPQIVNLAEEGSEDARDSQILI----QARLAEKLARKRSERFTYLVAAVMSSFGI 120

Query: 121 TSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLIL 180
           TSMAVMAVYYRF WQMEGGE+P SEMFGTF+LSVGAA                       
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEVPLSEMFGTFALSVGAA----------------------- 180

Query: 181 HNQFAINQVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALL 240
                   VGMEFWARWAHRALWH+SLWHMHESHH+PREGPFELND+FAIINAVPAIALL
Sbjct: 181 --------VGMEFWARWAHRALWHASLWHMHESHHRPREGPFELNDVFAIINAVPAIALL 240

Query: 241 SYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQL 300
           SYGFF+KGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQL
Sbjct: 241 SYGFFNKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQL 299

Query: 301 HHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 332
           HHSDKFNGVPYGLFLGPKE+EEVGGLEELEKEINRRIK
Sbjct: 301 HHSDKFNGVPYGLFLGPKEVEEVGGLEELEKEINRRIK 299

[Close]

BLAST of Cucsa.108110 vs. TrEMBL
Match: B9RIT5_RICCO (Beta-carotene hydroxylase, putative OS=Ricinus communis GN=RCOM_1582530 PE=3 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 3.1e-122
Identity = 229/331 (69.18%), Postives = 255/331 (77.04%), Query Frame = 1


Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60
           MAAGLSAA + KPF  ++ +S++ PKP    L PPS   NS F    RRKT   +C ++E
Sbjct: 1   MAAGLSAAPVFKPFR-YINASYILPKPLPTSLVPPSTRYNSLFLGG-RRKTGFAICFVLE 60

Query: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120
               S + +N  +EE+  V  QI +P V+ERLARK+SERFTYLVAAVMS+FGITSMAVMA
Sbjct: 61  DLKQSVETENPVEEENEKVNYQILTPRVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAIN 180
            YYRFYWQMEGGE+P +EMFGTFSLSVGAA                              
Sbjct: 121 CYYRFYWQMEGGEVPLAEMFGTFSLSVGAA------------------------------ 180

Query: 181 QVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240
            VGMEFWARWAHRALWH+SLWHMHESHH+PREGPFELND+FAI NAVPAI LL YGFF+K
Sbjct: 181 -VGMEFWARWAHRALWHASLWHMHESHHRPREGPFELNDVFAITNAVPAIGLLYYGFFNK 240

Query: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300
           GL PGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHS+KFN
Sbjct: 241 GLFPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSEKFN 298

Query: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIK 332
           GVPYGLFLGPKE+EEVGGLEELEKE +RR K
Sbjct: 301 GVPYGLFLGPKEIEEVGGLEELEKETSRRKK 298

[Close]

BLAST of Cucsa.108110 vs. TAIR10
Match: AT4G25700.1 (AT4G25700.1 beta-hydroxylase 1)

HSP 1 Score: 399.1 bits (1024), Expect = 2.8e-111
Identity = 217/348 (62.36%), Postives = 246/348 (70.69%), Query Frame = 1


Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPK---PPSPFLFPPSVFRNSRFQWKMRRKTLLTVCV 60
           MAAGLS A   KP H   +SS    +   P S   F PS+ R  RF          +VC 
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSLSGFSPSL-RFKRF----------SVCY 60

Query: 61  LVEGQNSSGKVDNLSDEESPIVVPQIPSPHVSERLA----RKKSERFTYLVAAVMSTFGI 120
           +VE +  +  ++N    ES      I + +++ RLA    RKKSER TYL+AA++S+FGI
Sbjct: 61  VVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGI 120

Query: 121 TSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLIL 180
           TSMAVMAVYYRF WQMEGGEI   EMFGTF+LSVGAA                       
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAA----------------------- 180

Query: 181 HNQFAINQVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALL 240
                   VGMEFWARWAHRALWH+SLW+MHESHHKPREGPFELND+FAI+NA PAI LL
Sbjct: 181 --------VGMEFWARWAHRALWHASLWNMHESHHKPREGPFELNDVFAIVNAGPAIGLL 240

Query: 241 SYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQL 300
           SYGFF+KGLVPGLCFGAGLGITVFG+AYMFVHDGLVHKRFPVGPIA+VPY RKVAAAHQL
Sbjct: 241 SYGFFNKGLVPGLCFGAGLGITVFGIAYMFVHDGLVHKRFPVGPIADVPYLRKVAAAHQL 300

Query: 301 HHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 342
           HH+DKFNGVPYGLFLGPKELEEVGG EEL+KEI+RRIK   + S  GS
Sbjct: 301 HHTDKFNGVPYGLFLGPKELEEVGGNEELDKEISRRIKSYKKASGSGS 306

[Close]

BLAST of Cucsa.108110 vs. TAIR10
Match: AT5G52570.1 (AT5G52570.1 beta-carotene hydroxylase 2)

HSP 1 Score: 397.5 bits (1020), Expect = 8.3e-111
Identity = 211/333 (63.36%), Postives = 241/333 (72.37%), Query Frame = 1


Query: 1   MAAGLSA-ATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLV 60
           MAAGLS  A   KP +    SS  +  P S  +FPPS+  N       RR+ +LTVC +V
Sbjct: 1   MAAGLSTIAVTLKPLN---RSSFSANHPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EGQNSSGKVDNLSDEESPIVVPQI-PSPHVSERLARKKSERFTYLVAAVMSTFGITSMAV 120
           E +  S  +D+ +  ES     +I  +  + ++  +KKSERFTYL+AAVMS+FGITSMA+
Sbjct: 61  EERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAI 120

Query: 121 MAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFA 180
           MAVYYRF WQM+GGE+   EMFGTF+LSVGAA                            
Sbjct: 121 MAVYYRFSWQMKGGEVSVLEMFGTFALSVGAA---------------------------- 180

Query: 181 INQVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFF 240
              VGMEFWARWAHRALWH SLW+MHESHHKPREG FELND+FAI NAVPAI LL YGF 
Sbjct: 181 ---VGMEFWARWAHRALWHDSLWNMHESHHKPREGAFELNDVFAITNAVPAIGLLYYGFL 240

Query: 241 HKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDK 300
           +KGLVPGLCFGAGLGIT+FGMAYMFVHDGLVHKRFPVGPIANVPY RKVAAAHQLHH+DK
Sbjct: 241 NKGLVPGLCFGAGLGITMFGMAYMFVHDGLVHKRFPVGPIANVPYLRKVAAAHQLHHTDK 294

Query: 301 FNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 332
           F GVPYGLFLGPKE+EEVGG EELEKEI+RRIK
Sbjct: 301 FKGVPYGLFLGPKEVEEVGGKEELEKEISRRIK 294

[Close]

BLAST of Cucsa.108110 vs. NCBI nr
Match: gi|449445997|ref|XP_004140758.1| (PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 624.8 bits (1610), Expect = 9.0e-176
Identity = 310/341 (90.91%), Postives = 310/341 (90.91%), Query Frame = 1


Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60
           MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120
           GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA
Sbjct: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAIN 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAA                              
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAA------------------------------ 180

Query: 181 QVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240
            VGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK
Sbjct: 181 -VGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240

Query: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300
           GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN
Sbjct: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300

Query: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 342
           GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS
Sbjct: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 310

[Close]

BLAST of Cucsa.108110 vs. NCBI nr
Match: gi|659077599|ref|XP_008439286.1| (PREDICTED: beta-carotene hydroxylase 2, chloroplastic [Cucumis melo])

HSP 1 Score: 591.3 bits (1523), Expect = 1.1e-165
Identity = 295/341 (86.51%), Postives = 301/341 (88.27%), Query Frame = 1


Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60
           MAA LSAA LPKPFHLFL+SSHLS KPP+PFLFPPSVF NSRFQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAALSAALLPKPFHLFLSSSHLSLKPPTPFLFPPSVFPNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120
            QNSSG+V +LSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAA+MS+FGITSMAVMA
Sbjct: 61  DQNSSGEVHSLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAIMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAIN 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAA                              
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAA------------------------------ 180

Query: 181 QVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240
            VGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK
Sbjct: 181 -VGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240

Query: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300
           GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN
Sbjct: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300

Query: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 342
           GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTA KS  GS
Sbjct: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTAPKSNYGS 310

[Close]

BLAST of Cucsa.108110 vs. NCBI nr
Match: gi|341657785|gb|AEK86567.1| (chloroplast beta-carotene hydroxylase [Cucurbita moschata])

HSP 1 Score: 555.4 bits (1430), Expect = 6.7e-155
Identity = 275/341 (80.65%), Postives = 294/341 (86.22%), Query Frame = 1


Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60
           MAA LSA   PKPFH+FLTSS+LSPKP +P LFP SVFRNSR QWKMRRKTLLTVCVLVE
Sbjct: 1   MAASLSAVLGPKPFHVFLTSSNLSPKPRTPLLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120
            Q+SS +V+NL+DE+  I+VPQIPSPHV+E+LARKKSERFTYLVAAVMS+FGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLLIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAIN 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAA                              
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAA------------------------------ 180

Query: 181 QVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240
            VGMEFWARWAHRALWH+SLWHMHESHHKPREGPFELND+FAI NAVPAIALLSYGFFHK
Sbjct: 181 -VGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAITNAVPAIALLSYGFFHK 240

Query: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300
           GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHS+K N
Sbjct: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSEKLN 300

Query: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 342
           GVPYGLFLGPKELEEVGGLEELEKEINRRIK TA+K I+GS
Sbjct: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKSTAKKPINGS 310

[Close]

BLAST of Cucsa.108110 vs. NCBI nr
Match: gi|694400895|ref|XP_009375525.1| (PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like [Pyrus x bretschneideri])

HSP 1 Score: 454.9 bits (1169), Expect = 1.2e-124
Identity = 233/338 (68.93%), Postives = 257/338 (76.04%), Query Frame = 1


Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60
           MA GL AAT P PF L + SSHL PKP    +F P++        + RRK   TV V++E
Sbjct: 1   MAVGLFAATTPTPFRL-IQSSHLLPKPNPATVFFPNLRHKKSALHRTRRKLCFTVYVVME 60

Query: 61  GQ-------NSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGI 120
            Q       NS+GK     D + PIV+P +    V+ERL+RKKSERFTYLVAAVMS+FGI
Sbjct: 61  DQKQITHLDNSAGKAPEADDSQIPIVIPSV---RVAERLSRKKSERFTYLVAAVMSSFGI 120

Query: 121 TSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLIL 180
           TSMAVMAVYYRFYWQMEGG +P SEM GTF+LSVGAA                       
Sbjct: 121 TSMAVMAVYYRFYWQMEGGNVPMSEMLGTFALSVGAA----------------------- 180

Query: 181 HNQFAINQVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALL 240
                   VGMEFWARWAH+ALWH+SLWHMHESHH+PREGPFELND+FAIINAVPAIALL
Sbjct: 181 --------VGMEFWARWAHKALWHASLWHMHESHHRPREGPFELNDVFAIINAVPAIALL 240

Query: 241 SYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQL 300
           +YGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPY RKVAAAHQL
Sbjct: 241 NYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYLRKVAAAHQL 300

Query: 301 HHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 332
           HHS+KF+GVPYGLFLGPKELEEVGGLEEL+KEINRRIK
Sbjct: 301 HHSEKFDGVPYGLFLGPKELEEVGGLEELDKEINRRIK 303

[Close]

BLAST of Cucsa.108110 vs. NCBI nr
Match: gi|694405407|ref|XP_009377554.1| (PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like [Pyrus x bretschneideri])

HSP 1 Score: 453.0 bits (1164), Expect = 4.7e-124
Identity = 232/338 (68.64%), Postives = 257/338 (76.04%), Query Frame = 1


Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60
           MA GL AAT P PF L + SS+L PKP    +F P++        + RRK   TV V++E
Sbjct: 1   MAVGLFAATTPTPFRL-IQSSYLLPKPKPATVFFPNLRHQKSALHRTRRKLCFTVYVVME 60

Query: 61  GQ-------NSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGI 120
            Q       NS+GK     D + PIV+P +    V+ERL+RKKSERFTYLVAAVMS+FGI
Sbjct: 61  DQKQITHLDNSAGKAPEADDSQIPIVIPSV---RVAERLSRKKSERFTYLVAAVMSSFGI 120

Query: 121 TSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLIL 180
           TSMAVMAVYYRFYWQMEGG +P SEM GTF+LSVGAA                       
Sbjct: 121 TSMAVMAVYYRFYWQMEGGNVPMSEMLGTFALSVGAA----------------------- 180

Query: 181 HNQFAINQVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALL 240
                   VGMEFWARWAH+ALWH+SLWHMHESHH+PREGPFELND+FAIINAVPAIALL
Sbjct: 181 --------VGMEFWARWAHKALWHASLWHMHESHHRPREGPFELNDVFAIINAVPAIALL 240

Query: 241 SYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQL 300
           +YGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPY RKVAAAHQL
Sbjct: 241 NYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYLRKVAAAHQL 300

Query: 301 HHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 332
           HHS+KF+GVPYGLFLGPKELEEVGGLEEL+KEINRRIK
Sbjct: 301 HHSEKFDGVPYGLFLGPKELEEVGGLEELDKEINRRIK 303

The following BLAST results are available for this feature:

BLAST of Cucsa.108110 vs. Swiss-Prot


Match Name	E-value	Identity	Description
BCH2_CAPAN	1.5e-117	67.64	Beta-carotene hydroxylase 2, chloroplastic (Fragment) OS=Capsicum annuum GN=CA2 ...	[more]
BCH_GENLU	2.9e-113	66.25	Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea GN=BHY PE=2 SV=1	[more]
BCH1_ARATH	5.0e-110	62.36	Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana GN=BETA-OHA...	[more]
BCH2_ARATH	1.5e-109	63.36	Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana GN=BETA-OHA...	[more]
BCH1_CAPAN	4.9e-105	58.45	Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum GN=CA1 PE=1 SV=1	[more]

BLAST of Cucsa.108110 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L606_CUCSA	6.3e-176	90.91	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G183920 PE=3 SV=1	[more]
A0A088CIY4_CUCME	7.7e-166	86.51	Beta-carotene hydroxylase OS=Cucumis melo var. makuwa GN=CHXB PE=2 SV=1	[more]
G1FQR0_CUCMO	4.7e-155	80.65	Chloroplast beta-carotene hydroxylase OS=Cucurbita moschata GN=CHYB PE=2 SV=1	[more]
A0A061G159_THECC	4.7e-123	69.82	Beta-hydroxylase 1, 1,B1,chy1,BCH1 isoform 1 OS=Theobroma cacao GN=TCM_015247 PE...	[more]
B9RIT5_RICCO	3.1e-122	69.18	Beta-carotene hydroxylase, putative OS=Ricinus communis GN=RCOM_1582530 PE=3 SV=...	[more]

BLAST of Cucsa.108110 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G25700.1	2.8e-111	62.36	beta-hydroxylase 1	[more]
AT5G52570.1	8.3e-111	63.36	beta-carotene hydroxylase 2	[more]

BLAST of Cucsa.108110 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449445997\|ref\|XP_004140758.1\|	9.0e-176	90.91	PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like [Cucumis sativus]	[more]
gi\|659077599\|ref\|XP_008439286.1\|	1.1e-165	86.51	PREDICTED: beta-carotene hydroxylase 2, chloroplastic [Cucumis melo]	[more]
gi\|341657785\|gb\|AEK86567.1\|	6.7e-155	80.65	chloroplast beta-carotene hydroxylase [Cucurbita moschata]	[more]
gi\|694400895\|ref\|XP_009375525.1\|	1.2e-124	68.93	PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like [Pyrus x bretschneide...	[more]
gi\|694405407\|ref\|XP_009377554.1\|	4.7e-124	68.64	PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like [Pyrus x bretschneide...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006694	Fatty_acid_hydroxylase
IPR006694	Fatty_acid_hydroxylase

Vocabulary: **Molecular Function**
Term	Definition
GO:0005506	iron ion binding
GO:0016491	oxidoreductase activity
GO:0005506	iron ion binding
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006633	fatty acid biosynthetic process
GO:0055114	oxidation-reduction process
GO:0006633	fatty acid biosynthetic process
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006633	fatty acid biosynthetic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.108110.2	Cucsa.108110.2	mRNA
Cucsa.108110.1	Cucsa.108110.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 149..276 score: 7.4
None	No IPR available	PANTHER	PTHR31899	FAMILY NOT NAMED	coord: 2..309 score: 4.0E
None	No IPR available	PANTHER	PTHR31899:SF1	BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTIC-RELATED	coord: 2..309 score: 4.0E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.108110	Cla006149	Watermelon (97103) v1	cgywmB152
Cucsa.108110	Csa3G183920	Cucumber (Chinese Long) v2	cgycuB132
Cucsa.108110	MELO3C006938	Melon (DHL92) v3.5.1	cgymeB142
Cucsa.108110	ClCG05G008380	Watermelon (Charleston Gray)	cgywcgB152
Cucsa.108110	CSPI03G17250	Wild cucumber (PI 183967)	cgycpiB133
Cucsa.108110	CmaCh16G001000	Cucurbita maxima (Rimu)	cgycmaB0248
Cucsa.108110	CmaCh18G012680	Cucurbita maxima (Rimu)	cgycmaB0249
Cucsa.108110	CmoCh18G012980	Cucurbita moschata (Rifu)	cgycmoB0248
Cucsa.108110	CmoCh16G001080	Cucurbita moschata (Rifu)	cgycmoB0247
Cucsa.108110	Lsi05G011960	Bottle gourd (USVL1VR-Ls)	cgylsiB143
Cucsa.108110	Cp4.1LG09g00740	Cucurbita pepo (Zucchini)	cgycpeB0240
Cucsa.108110	Cp4.1LG14g06240	Cucurbita pepo (Zucchini)	cgycpeB0241
Cucsa.108110	MELO3C006938.2	Melon (DHL92) v3.6.1	cgymedB145
Cucsa.108110	CsaV3_3G017450	Cucumber (Chinese Long) v3	cgycucB137
Cucsa.108110	Cla97C05G090480	Watermelon (97103) v2	cgywmbB147
Cucsa.108110	BhiUN557G10	Wax gourd	cgywgoB168
Cucsa.108110	Carg22126	Silver-seed gourd	carcgyB0828
Cucsa.108110	Carg15111	Silver-seed gourd	carcgyB1105

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.108110	Cucurbita maxima (Rimu)	cgycmaB0251
Cucsa.108110	Cucurbita moschata (Rifu)	cgycmoB0250
Cucsa.108110	Melon (DHL92) v3.5.1	cgymeB145
Cucsa.108110	Cucurbita pepo (Zucchini)	cgycpeB0244
Cucsa.108110	Melon (DHL92) v3.6.1	cgymedB142
Cucsa.108110	Silver-seed gourd	carcgyB0794