Cp4.1LG14g06240 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG14g06240
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Beta-carotene hydroxylase
Location	Cp4.1LG14 : 468453 .. 471329 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAATGAGAATGAATTTAAAAGAGAGAGAAAATAAAAAAAAATGGCAATTTCGTATATAAACGAAAGAAGGGCAGCAAACGTCCATTTTGTCCGCTCAGAGTAATTTAGTCCCCCATTTCACTGACGAAAATTTGTGGTCTCTGTTCCTTCAACGTCTATTAAAGCCCTCTCTCCGATAAGCTTCTCCCTCTTCCACCGTCTTTTCACTTTTCACCTTCATCTCTCCCATGGCGGCCGCCCTCTCCGCCGCCTTAGGGCCCAAACCACTCCATCTCTTCCTTGCTTCCTCCCATCTCTCCCCTAAACCTCGAACTCCGTTTCTGTTTCCACCTTCAGTCTTCCGGAACAGTAGAATCCAACGGAAGATACGGAGAAAAACGCTGTTCACTGTCTGCGTGCTCGTTGAGGATCAAAATAGCTCTGGCGAGGTGGAGAGTCTTTCCGATGAAGAACCGAAGATTGTAGTCCCCCAGATCCCATCGCCTTATGTTGCACAAAGACTGGCAAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCTGTCATGGCGGTTTATTACCGATTTTACTGGCAAATGGAGGTCCTCTCTTTCTCTATCGAGAAACTTTAATTGTTTTTGTTCTCATTGGCGCCGTATTCTTCTGATTGAATATTGAATTGCGATTGTTTTATTTGGCGGTGTGTAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCTGTTGGTGCCGCTGTACGTTTTCGATTTCATCTTTGTATTCTCTACTTCTCGCGAAGAATTCGATTTGCTATAAGCTAATCTTTCACAATCAATTTGGGAATATCAGGTCGGCATGGAGTTTTGGGCGAGATGGGCTCATAGGGCTCTCTGGCACTCTTCCTTGTGGCATATGCACGAGGTTTCTAATCCTTCTTCTCTTTAGAACATTTGAATCCACTGTGAAGCTTAGTTTCATATCTAACAAACAGTATCCAAAGGGAAAATAGATTCGTGACGCTTGGACTTGTAATTGCCGTAGTCGCACCATTTCTAGACTAATCCTTTCTTCATAGATTATTCTTTATTGGCTGCTGAGAAATTTAATTAACAATCTCCGTTTTCCATGTTCAATCTCGAAGCAAACAAACAACATTTAATTTATTTTTTTAAAAAGTCGTTGACGCTTGGACTTTGTTGATCTTGATTATTACAGTCGCACCATAAACCAAGAGAAGGACCGTTCGAACTGAACGACGTCTTCGCCATTATCAATGCCGTGCCCGCCATAGCTCTCCTCTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCCGGTCTCTGCTTCGGCGCTGTAAATCTCTCTTTCTCTCTCATAATCTTTCAGAATCTCAGTTTATTCATATTCCAACTGTTCAATGGTGTTATTTAATCACTGGGGATTAGCGCTAATGATATGTTAAATGACAAAATTAGCCTTAGAAAATGTGGTATTGAGAAAGGTTTGACACGCTGGCGGGTTGTGGCAGGGCCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTGGGTCCCATTGCCAACGTGCCCTATTTCAGAAAGGTCGCTGCTGCTCACCAGGTAAATTTTATTTATTTATTTAAATCTTATTTTGATCTCAAATTTGATACTCTGTTTACTCCCTACTGTGTTGACTTTTCATTAATGATCTAACAAAATCTCTATTATGTTCCAAAACTTAAGATATTTAGATCACTAGTGTAGATTCCTGTTAATTAATTCTAAATTTATGAATATCAAGATGCTAGACATTAGCGTAGAATTGACTTAGATACAAAAATATGATTTTAAGTCAGCCAACTAATGGGATCTTGGTAATAATTTAAAGAATTACTCTAATAATAATAATTGTAATAAGAAAATAGCTATGGAATTTGGAACTATAATAGAATCCATAGACTTTCCATCATTTTTAATAATGTCACATTAAATTATGTTTCTTAGGGTTAAAATTAAAATATTGTCTCTGATTTGAATATTGAAGCTTATGAGTTTTTGTTTTTCTTGGCTTAAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCATATGGACTGTTTTTAGGTCCGAAGGTCAGTTTCGGGTCTATCTTTCTATATATATATATATATATATCTATTAAAATTTTAATAAATGAATTTTGTAAAATTTAAATGATGGGTGCAGGAATTAGAGGAAGTGGGAGGGGAAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATTGACGACAACAAAATCATCAATCAACGGCTCTTGATGAACCAATATTGTGTGAAAATGAGACGACGAAAACAAAGGGGTGGAAATATAGGCCTCCTGAACAGTAACTTTTCTGTTTTTTAACATTGAAAAGTGAAATATTATGTAGAACAGGGGGTGGTATTTTTTTATCTTCTTTTACTGTATGAAGCAAAGCGGTAGAGAAATGAGAGTGAGTGAATTGGGAGGAAGATTTTTACTGTGACTCGCTCTCTACCGTTCCGGTTTTTGTGACAGTAAAGAAAATTTGGTTGGGGATAGTGTAATTATGTATAAATAATAATAATAATAATAATAATTAAAGAAAACTAGTAAATAGCGTAACTTTACACGCAATGGATGACCGATTTAGTTCCGCTTTCAGTTTCCTGCCGATGCGGCCACAATCATCGTAAGAACTCTCGAGTTATGCTGAAATTTCGGACAAACAGCCAAGCAGTTCAGTGCTGAAATGGGTCCACCGGAATTTCATAATAAGGTCGGTGAAAAGTTTC

mRNA sequence

AAAATGAGAATGAATTTAAAAGAGAGAGAAAATAAAAAAAAATGGCAATTTCGTATATAAACGAAAGAAGGGCAGCAAACGTCCATTTTGTCCGCTCAGAGTAATTTAGTCCCCCATTTCACTGACGAAAATTTGTGGTCTCTGTTCCTTCAACGTCTATTAAAGCCCTCTCTCCGATAAGCTTCTCCCTCTTCCACCGTCTTTTCACTTTTCACCTTCATCTCTCCCATGGCGGCCGCCCTCTCCGCCGCCTTAGGGCCCAAACCACTCCATCTCTTCCTTGCTTCCTCCCATCTCTCCCCTAAACCTCGAACTCCGTTTCTGTTTCCACCTTCAGTCTTCCGGAACAGTAGAATCCAACGGAAGATACGGAGAAAAACGCTGTTCACTGTCTGCGTGCTCGTTGAGGATCAAAATAGCTCTGGCGAGGTGGAGAGTCTTTCCGATGAAGAACCGAAGATTGTAGTCCCCCAGATCCCATCGCCTTATGTTGCACAAAGACTGGCAAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCTGTCATGGCGGTTTATTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCTGTTGGTGCCGCTGTCGGCATGGAGTTTTGGGCGAGATGGGCTCATAGGGCTCTCTGGCACTCTTCCTTGTGGCATATGCACGAGTCGCACCATAAACCAAGAGAAGGACCGTTCGAACTGAACGACGTCTTCGCCATTATCAATGCCGTGCCCGCCATAGCTCTCCTCTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCCGGTCTCTGCTTCGGCGCTGGCCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTGGGTCCCATTGCCAACGTGCCCTATTTCAGAAAGGTCGCTGCTGCTCACCAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCATATGGACTGTTTTTAGGTCCGAAGGAATTAGAGGAAGTGGGAGGGGAAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATTGACGACAACAAAATCATCAATCAACGGCTCTTGATGAACCAATATTGTGTGAAAATGAGACGACGAAAACAAAGGGGTGGAAATATAGGCCTCCTGAACAGTAACTTTTCTGTTTTTTAACATTGAAAAGTGAAATATTATGTAGAACAGGGGGTGGTATTTTTTTATCTTCTTTTACTGTATGAAGCAAAGCGGTAGAGAAATGAGAGTGAGTGAATTGGGAGGAAGATTTTTACTGTGACTCGCTCTCTACCGTTCCGGTTTTTGTGACAGTAAAGAAAATTTGGTTGGGGATAGTGTAATTATGTATAAATAATAATAATAATAATAATAATTAAAGAAAACTAGTAAATAGCGTAACTTTACACGCAATGGATGACCGATTTAGTTCCGCTTTCAGTTTCCTGCCGATGCGGCCACAATCATCGTAAGAACTCTCGAGTTATGCTGAAATTTCGGACAAACAGCCAAGCAGTTCAGTGCTGAAATGGGTCCACCGGAATTTCATAATAAGGTCGGTGAAAAGTTTC

Coding sequence (CDS)

ATGGCGGCCGCCCTCTCCGCCGCCTTAGGGCCCAAACCACTCCATCTCTTCCTTGCTTCCTCCCATCTCTCCCCTAAACCTCGAACTCCGTTTCTGTTTCCACCTTCAGTCTTCCGGAACAGTAGAATCCAACGGAAGATACGGAGAAAAACGCTGTTCACTGTCTGCGTGCTCGTTGAGGATCAAAATAGCTCTGGCGAGGTGGAGAGTCTTTCCGATGAAGAACCGAAGATTGTAGTCCCCCAGATCCCATCGCCTTATGTTGCACAAAGACTGGCAAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCTGTCATGGCGGTTTATTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCTGTTGGTGCCGCTGTCGGCATGGAGTTTTGGGCGAGATGGGCTCATAGGGCTCTCTGGCACTCTTCCTTGTGGCATATGCACGAGTCGCACCATAAACCAAGAGAAGGACCGTTCGAACTGAACGACGTCTTCGCCATTATCAATGCCGTGCCCGCCATAGCTCTCCTCTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCCGGTCTCTGCTTCGGCGCTGGCCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTGGGTCCCATTGCCAACGTGCCCTATTTCAGAAAGGTCGCTGCTGCTCACCAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCATATGGACTGTTTTTAGGTCCGAAGGAATTAGAGGAAGTGGGAGGGGAAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATTGACGACAACAAAATCATCAATCAACGGCTCTTGA

Protein sequence

MAAALSAALGPKPLHLFLASSHLSPKPRTPFLFPPSVFRNSRIQRKIRRKTLFTVCVLVEDQNSSGEVESLSDEEPKIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGEEELEKEINRRIKLTTTKSSINGS

Homology

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BLAST of Cp4.1LG14g06240 vs. Swiss-Prot
Match: BCH2_CAPAN (Beta-carotene hydroxylase 2, chloroplastic (Fragment) OS=Capsicum annuum GN=CA2 PE=2 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 1.8e-122
Identity = 213/277 (76.90%), Postives = 239/277 (86.28%), Query Frame = 1


Query: 25  PKPRTPFLFPPSVFR-NSRIQRKIRRKTLFTVCVLVEDQNSSGEVESLSDEEPKIVVPQI 84
           P P TP ++P + F  N     + RR+  FTVC ++ED     + E+  ++    +  QI
Sbjct: 35  PTPTTPSVYPITPFSPNLGSILRCRRRPSFTVCFVLEDDKFKTQFEAGEEDIEMKIEEQI 94

Query: 85  PSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTF 144
            +  +A++LARK+SERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGE+PFSEMFGTF
Sbjct: 95  SATRLAEKLARKKSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEVPFSEMFGTF 154

Query: 145 SLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDVFAIINAVPAIALLS 204
           +LSVGAAVGMEFWARWAH+ALWH+SLWHMHESHHKPREGPFELNDVFAIINAVPAIALL 
Sbjct: 155 ALSVGAAVGMEFWARWAHKALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALLD 214

Query: 205 YGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLH 264
           YGFFHKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGP+ANVPY RKVAAAH LH
Sbjct: 215 YGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPVANVPYLRKVAAAHSLH 274

Query: 265 HSDKFNGVPYGLFLGPKELEEVGGEEELEKEINRRIK 301
           HS+KFNGVPYGLFLGPKELEEVGG EELEKE+NRR +
Sbjct: 275 HSEKFNGVPYGLFLGPKELEEVGGLEELEKEVNRRTR 311

[Close]

BLAST of Cp4.1LG14g06240 vs. Swiss-Prot
Match: BCH_GENLU (Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea GN=BHY PE=2 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 2.1e-115
Identity = 216/303 (71.29%), Postives = 244/303 (80.53%), Query Frame = 1


Query: 6   SAALGPKPLHLFLASSHLSPKPRTPFLFPPSVFRNSRIQRKIRRKTLFTVCVLVEDQNSS 65
           S++L PK       SS +S    T  +FPP  F+      + R K   TVC ++E++   
Sbjct: 23  SSSLTPK-------SSPVSTSTPTLVVFPP--FKLVSKSLRTRSKPRLTVCFVLEEKELR 82

Query: 66  GEVESLSDEEP------KIVVPQIPSPY--VAQRLARKRSERFTYLVAAVMSSFGITSMA 125
           G++   SD++       K    +I +    +AQ+LARK+SERFTYLVAAVMSSFGITSMA
Sbjct: 83  GKLVVASDDDDGAGEVRKQREKEISASAEKLAQKLARKKSERFTYLVAAVMSSFGITSMA 142

Query: 126 VMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHH 185
           V++VYYRF WQMEGGEIP SEMFGTF+LSVGAAVGMEFWARWAH ALWH+SLWHMHESHH
Sbjct: 143 VLSVYYRFSWQMEGGEIPLSEMFGTFALSVGAAVGMEFWARWAHEALWHASLWHMHESHH 202

Query: 186 KPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGL 245
           KPREGPFELND+FAIINAVPAIALLSYGFFHKGL+PGLCFGAGLGITVFGMAYMFVHDGL
Sbjct: 203 KPREGPFELNDIFAIINAVPAIALLSYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGL 262

Query: 246 VHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGEEELEKEINR 301
           VHKRFPVGPIA+VPYFR+VAAAH LHHSDKFNGVPYGLFLGPKELEEVGG + LE EINR
Sbjct: 263 VHKRFPVGPIADVPYFRRVAAAHTLHHSDKFNGVPYGLFLGPKELEEVGGLQVLEMEINR 316

[Close]

BLAST of Cp4.1LG14g06240 vs. Swiss-Prot
Match: BCH1_ARATH (Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana GN=BETA-OHASE 1 PE=1 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 8.9e-114
Identity = 220/318 (69.18%), Postives = 251/318 (78.93%), Query Frame = 1


Query: 1   MAAALSAALGPKPLHLFLASSHLSPKPRTPFL---FPPSVFRNSRIQRKIRRKTLFTVCV 60
           MAA LS A+  KPLH   +SS    + R P     F PS+    R +R       F+VC 
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSLSGFSPSL----RFKR-------FSVCY 60

Query: 61  LVEDQNSSGEVESLSDEEPKIVVPQIPSPYVAQRLA----RKRSERFTYLVAAVMSSFGI 120
           +VE++  +  +E+    E       I + Y+A RLA    RK+SER TYL+AA++SSFGI
Sbjct: 61  VVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGI 120

Query: 121 TSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMH 180
           TSMAVMAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWH+SLW+MH
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMH 180

Query: 181 ESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFV 240
           ESHHKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGAGLGITVFG+AYMFV
Sbjct: 181 ESHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAGLGITVFGIAYMFV 240

Query: 241 HDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGEEELEK 300
           HDGLVHKRFPVGPIA+VPY RKVAAAHQLHH+DKFNGVPYGLFLGPKELEEVGG EEL+K
Sbjct: 241 HDGLVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFNGVPYGLFLGPKELEEVGGNEELDK 300

Query: 301 EINRRIKLTTTKSSINGS 312
           EI+RRIK +  K+S +GS
Sbjct: 301 EISRRIK-SYKKASGSGS 306

[Close]

BLAST of Cp4.1LG14g06240 vs. Swiss-Prot
Match: BCH2_ARATH (Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana GN=BETA-OHASE 2 PE=2 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 1.1e-111
Identity = 211/311 (67.85%), Postives = 245/311 (78.78%), Query Frame = 1


Query: 1   MAAALSA-ALGPKPLHLFLASSHLSPKPRTPFLFPPSVFRNSRIQRKIRRKTLFTVCVLV 60
           MAA LS  A+  KPL+    SS  +  P +  +FPPS+  N       RR+ + TVC +V
Sbjct: 1   MAAGLSTIAVTLKPLN---RSSFSANHPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVESLSDEEPKIVVPQ---IPSPYVAQRLARKRSERFTYLVAAVMSSFGITSM 120
           E++  S  ++   D +P+        + +  + ++  +K+SERFTYL+AAVMSSFGITSM
Sbjct: 61  EERKQSSPMDD--DNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSM 120

Query: 121 AVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESH 180
           A+MAVYYRF WQM+GGE+   EMFGTF+LSVGAAVGMEFWARWAHRALWH SLW+MHESH
Sbjct: 121 AIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHESH 180

Query: 181 HKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDG 240
           HKPREG FELNDVFAI NAVPAI LL YGF +KGLVPGLCFGAGLGIT+FGMAYMFVHDG
Sbjct: 181 HKPREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHDG 240

Query: 241 LVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGEEELEKEIN 300
           LVHKRFPVGPIANVPY RKVAAAHQLHH+DKF GVPYGLFLGPKE+EEVGG+EELEKEI+
Sbjct: 241 LVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEIS 300

Query: 301 RRIKLTTTKSS 308
           RRIKL    SS
Sbjct: 301 RRIKLYNKGSS 301

[Close]

BLAST of Cp4.1LG14g06240 vs. Swiss-Prot
Match: BCH1_CAPAN (Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum GN=CA1 PE=1 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 4.3e-108
Identity = 202/312 (64.74%), Postives = 240/312 (76.92%), Query Frame = 1


Query: 1   MAAALSAALGPKPLHL----FLASSHLSPKPRTPFLFPPSVFRNSRIQRKIRRKTLFTVC 60
           MAA +S +   + + L    F A  + +  P  P LF   +  N     + RRK     C
Sbjct: 1   MAAEISISASSRAICLQRNPFPAPKYFATAP--PLLFFSPLTCNLDAILRSRRKPRLAAC 60

Query: 61  VLVEDQN-------SSGEVESLSDE-EPKIVVPQIPSPYVAQRLARKRSERFTYLVAAVM 120
            +++D            + E++ DE E +    +  +  +A++ ARK+SERFTYLVAAVM
Sbjct: 61  FVLKDDKLYTAQSGKQSDTEAIGDEIEVETNEEKSLAVRLAEKFARKKSERFTYLVAAVM 120

Query: 121 SSFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSS 180
           SS GITSMAV++VYYRF WQMEGGE+PFSEMF TF+L+ GAA+GME+WARWAHRALWH+S
Sbjct: 121 SSLGITSMAVISVYYRFSWQMEGGEMPFSEMFCTFALAFGAAIGMEYWARWAHRALWHAS 180

Query: 181 LWHMHESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGM 240
           LWHMHESHH+PREGPFELND+FAIINAVPAIA  S+GF HKGL+PG+CFGAGLGITVFGM
Sbjct: 181 LWHMHESHHRPREGPFELNDIFAIINAVPAIAFFSFGFNHKGLIPGICFGAGLGITVFGM 240

Query: 241 AYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGE 300
           AYMFVHDGLVHKRFPVGPIA VPYF++VAAAHQLHHSDKF+GVPYGLFLGPKELEEVG  
Sbjct: 241 AYMFVHDGLVHKRFPVGPIAKVPYFQRVAAAHQLHHSDKFDGVPYGLFLGPKELEEVGVI 300

[Close]

BLAST of Cp4.1LG14g06240 vs. TrEMBL
Match: A0A088CIY4_CUCME (Beta-carotene hydroxylase OS=Cucumis melo var. makuwa GN=CHXB PE=2 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 1.3e-159
Identity = 283/307 (92.18%), Postives = 292/307 (95.11%), Query Frame = 1


Query: 1   MAAALSAALGPKPLHLFLASSHLSPKPRTPFLFPPSVFRNSRIQRKIRRKTLFTVCVLVE 60
           MAAALSAAL PKP HLFL+SSHLS KP TPFLFPPSVF NSR Q K+RRKTL TVCVLVE
Sbjct: 1   MAAALSAALLPKPFHLFLSSSHLSLKPPTPFLFPPSVFPNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVESLSDEEPKIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQNSSGEV SLSDEE  IVVPQIPSP+V++RLARK+SERFTYLVAA+MSSFGITSMAVMA
Sbjct: 61  DQNSSGEVHSLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAIMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELND+FAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGEEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 LTTTKSS 308
            T  KS+
Sbjct: 301 WTAPKSN 307

[Close]

BLAST of Cp4.1LG14g06240 vs. TrEMBL
Match: A0A0A0L606_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G183920 PE=3 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 1.4e-158
Identity = 282/311 (90.68%), Postives = 294/311 (94.53%), Query Frame = 1


Query: 1   MAAALSAALGPKPLHLFLASSHLSPKPRTPFLFPPSVFRNSRIQRKIRRKTLFTVCVLVE 60
           MAA LSAA  PKP HLFL SSHLSPKP +PFLFPPSVFRNSR Q K+RRKTL TVCVLVE
Sbjct: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVESLSDEEPKIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120
            QNSSG+V++LSDEE  IVVPQIPSP+V++RLARK+SERFTYLVAAVMS+FGITSMAVMA
Sbjct: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELND+FAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGEEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 LTTTKSSINGS 312
            T  K SI+GS
Sbjct: 301 WTARK-SIDGS 310

[Close]

BLAST of Cp4.1LG14g06240 vs. TrEMBL
Match: G1FQR0_CUCMO (Chloroplast beta-carotene hydroxylase OS=Cucurbita moschata GN=CHYB PE=2 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 8.6e-156
Identity = 276/311 (88.75%), Postives = 294/311 (94.53%), Query Frame = 1


Query: 1   MAAALSAALGPKPLHLFLASSHLSPKPRTPFLFPPSVFRNSRIQRKIRRKTLFTVCVLVE 60
           MAA+LSA LGPKP H+FL SS+LSPKPRTP LFP SVFRNSRIQ K+RRKTL TVCVLVE
Sbjct: 1   MAASLSAVLGPKPFHVFLTSSNLSPKPRTPLLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVESLSDEEPKIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVE+L+DE+  I+VPQIPSP+VA++LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLLIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGEEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHS+K NGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKLNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 LTTTKSSINGS 312
            +T K  INGS
Sbjct: 301 -STAKKPINGS 310

[Close]

BLAST of Cp4.1LG14g06240 vs. TrEMBL
Match: A0A061G159_THECC (Beta-hydroxylase 1, 1,B1,chy1,BCH1 isoform 1 OS=Theobroma cacao GN=TCM_015247 PE=3 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 6.0e-125
Identity = 236/307 (76.87%), Postives = 260/307 (84.69%), Query Frame = 1


Query: 1   MAAALSAALGPKPLHLFLASSHLSPKPRTPFLFPPSVFRNSRIQRKIRRKTLFTVCVLVE 60
           MAA LSAA+ PKP   FL SSHL PKP TP   P  + R  +     RRK  F VC ++E
Sbjct: 1   MAAGLSAAITPKPFRSFL-SSHLEPKP-TPLFHP--LLRLQKTLYGARRKRSFAVCFVLE 60

Query: 61  DQNSSG-EVESLSDE------EPKIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGI 120
           +Q  S  ++ +L++E      + +I++       +A++LARKRSERFTYLVAAVMSSFGI
Sbjct: 61  EQKQSAPQIVNLAEEGSEDARDSQILI----QARLAEKLARKRSERFTYLVAAVMSSFGI 120

Query: 121 TSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMH 180
           TSMAVMAVYYRF WQMEGGE+P SEMFGTF+LSVGAAVGMEFWARWAHRALWH+SLWHMH
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEVPLSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMH 180

Query: 181 ESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFV 240
           ESHH+PREGPFELNDVFAIINAVPAIALLSYGFF+KGLVPGLCFGAGLGITVFGMAYMFV
Sbjct: 181 ESHHRPREGPFELNDVFAIINAVPAIALLSYGFFNKGLVPGLCFGAGLGITVFGMAYMFV 240

Query: 241 HDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGEEELEK 300
           HDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKE+EEVGG EELEK
Sbjct: 241 HDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKEVEEVGGLEELEK 299

[Close]

BLAST of Cp4.1LG14g06240 vs. TrEMBL
Match: M5X1V0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009001mg PE=3 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 5.1e-124
Identity = 229/306 (74.84%), Postives = 255/306 (83.33%), Query Frame = 1


Query: 1   MAAALSAALGPKPLHLFLASSHLSPKPRTPFLFPPSVFRN--SRIQRKIRRKTLFTVCVL 60
           MAA   AA  P P+ L + SS+L  KP     FPPS  R+  S +  + RRK  FTV V+
Sbjct: 1   MAAGHFAATTPNPIRL-IQSSYLLAKPNPTIFFPPSNLRHQKSALHYRARRKLCFTVYVV 60

Query: 61  VEDQNSSGEVESLSDEEPKIVVPQIP----SPYVAQRLARKRSERFTYLVAAVMSSFGIT 120
           +EDQ  S  +E+ +++  +    QIP    S  VA++L+RK+SERFTYLVAAVMSSFGIT
Sbjct: 61  MEDQKQSTHLENCTEKAQEAAQSQIPIVIPSVRVAEKLSRKKSERFTYLVAAVMSSFGIT 120

Query: 121 SMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHE 180
           SMAVMAVYYRFYWQMEGG +P SEM GTF+LSVGAAVGMEFWARWAH+ALWH+SLWHMHE
Sbjct: 121 SMAVMAVYYRFYWQMEGGNVPLSEMLGTFALSVGAAVGMEFWARWAHKALWHASLWHMHE 180

Query: 181 SHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVH 240
           SHH+PREGPFELNDVFA+INAVPAIALL+YGFFHKGLVPGLCFGAGLGITVFGMAYMFVH
Sbjct: 181 SHHRPREGPFELNDVFAVINAVPAIALLNYGFFHKGLVPGLCFGAGLGITVFGMAYMFVH 240

Query: 241 DGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGEEELEKE 300
           DGLVHKRFPVGPIANVPY RKVAAAHQLHHS+KF GVPYGLFLGPKELEEVGG EELEKE
Sbjct: 241 DGLVHKRFPVGPIANVPYLRKVAAAHQLHHSEKFEGVPYGLFLGPKELEEVGGLEELEKE 300

[Close]

BLAST of Cp4.1LG14g06240 vs. TAIR10
Match: AT4G25700.1 (AT4G25700.1 beta-hydroxylase 1)

HSP 1 Score: 411.4 bits (1056), Expect = 5.0e-115
Identity = 220/318 (69.18%), Postives = 251/318 (78.93%), Query Frame = 1


Query: 1   MAAALSAALGPKPLHLFLASSHLSPKPRTPFL---FPPSVFRNSRIQRKIRRKTLFTVCV 60
           MAA LS A+  KPLH   +SS    + R P     F PS+    R +R       F+VC 
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSLSGFSPSL----RFKR-------FSVCY 60

Query: 61  LVEDQNSSGEVESLSDEEPKIVVPQIPSPYVAQRLA----RKRSERFTYLVAAVMSSFGI 120
           +VE++  +  +E+    E       I + Y+A RLA    RK+SER TYL+AA++SSFGI
Sbjct: 61  VVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGI 120

Query: 121 TSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMH 180
           TSMAVMAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWH+SLW+MH
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMH 180

Query: 181 ESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFV 240
           ESHHKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGAGLGITVFG+AYMFV
Sbjct: 181 ESHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAGLGITVFGIAYMFV 240

Query: 241 HDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGEEELEK 300
           HDGLVHKRFPVGPIA+VPY RKVAAAHQLHH+DKFNGVPYGLFLGPKELEEVGG EEL+K
Sbjct: 241 HDGLVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFNGVPYGLFLGPKELEEVGGNEELDK 300

Query: 301 EINRRIKLTTTKSSINGS 312
           EI+RRIK +  K+S +GS
Sbjct: 301 EISRRIK-SYKKASGSGS 306

[Close]

BLAST of Cp4.1LG14g06240 vs. TAIR10
Match: AT5G52570.1 (AT5G52570.1 beta-carotene hydroxylase 2)

HSP 1 Score: 404.4 bits (1038), Expect = 6.1e-113
Identity = 211/311 (67.85%), Postives = 245/311 (78.78%), Query Frame = 1


Query: 1   MAAALSA-ALGPKPLHLFLASSHLSPKPRTPFLFPPSVFRNSRIQRKIRRKTLFTVCVLV 60
           MAA LS  A+  KPL+    SS  +  P +  +FPPS+  N       RR+ + TVC +V
Sbjct: 1   MAAGLSTIAVTLKPLN---RSSFSANHPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVESLSDEEPKIVVPQ---IPSPYVAQRLARKRSERFTYLVAAVMSSFGITSM 120
           E++  S  ++   D +P+        + +  + ++  +K+SERFTYL+AAVMSSFGITSM
Sbjct: 61  EERKQSSPMDD--DNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSM 120

Query: 121 AVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESH 180
           A+MAVYYRF WQM+GGE+   EMFGTF+LSVGAAVGMEFWARWAHRALWH SLW+MHESH
Sbjct: 121 AIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHESH 180

Query: 181 HKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDG 240
           HKPREG FELNDVFAI NAVPAI LL YGF +KGLVPGLCFGAGLGIT+FGMAYMFVHDG
Sbjct: 181 HKPREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHDG 240

Query: 241 LVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGEEELEKEIN 300
           LVHKRFPVGPIANVPY RKVAAAHQLHH+DKF GVPYGLFLGPKE+EEVGG+EELEKEI+
Sbjct: 241 LVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEIS 300

Query: 301 RRIKLTTTKSS 308
           RRIKL    SS
Sbjct: 301 RRIKLYNKGSS 301

[Close]

BLAST of Cp4.1LG14g06240 vs. NCBI nr
Match: gi|659077599|ref|XP_008439286.1| (PREDICTED: beta-carotene hydroxylase 2, chloroplastic [Cucumis melo])

HSP 1 Score: 570.5 bits (1469), Expect = 1.8e-159
Identity = 283/307 (92.18%), Postives = 292/307 (95.11%), Query Frame = 1


Query: 1   MAAALSAALGPKPLHLFLASSHLSPKPRTPFLFPPSVFRNSRIQRKIRRKTLFTVCVLVE 60
           MAAALSAAL PKP HLFL+SSHLS KP TPFLFPPSVF NSR Q K+RRKTL TVCVLVE
Sbjct: 1   MAAALSAALLPKPFHLFLSSSHLSLKPPTPFLFPPSVFPNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVESLSDEEPKIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQNSSGEV SLSDEE  IVVPQIPSP+V++RLARK+SERFTYLVAA+MSSFGITSMAVMA
Sbjct: 61  DQNSSGEVHSLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAIMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELND+FAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGEEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 LTTTKSS 308
            T  KS+
Sbjct: 301 WTAPKSN 307

[Close]

BLAST of Cp4.1LG14g06240 vs. NCBI nr
Match: gi|449445997|ref|XP_004140758.1| (PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 567.0 bits (1460), Expect = 2.0e-158
Identity = 282/311 (90.68%), Postives = 294/311 (94.53%), Query Frame = 1


Query: 1   MAAALSAALGPKPLHLFLASSHLSPKPRTPFLFPPSVFRNSRIQRKIRRKTLFTVCVLVE 60
           MAA LSAA  PKP HLFL SSHLSPKP +PFLFPPSVFRNSR Q K+RRKTL TVCVLVE
Sbjct: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVESLSDEEPKIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120
            QNSSG+V++LSDEE  IVVPQIPSP+V++RLARK+SERFTYLVAAVMS+FGITSMAVMA
Sbjct: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELND+FAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGEEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 LTTTKSSINGS 312
            T  K SI+GS
Sbjct: 301 WTARK-SIDGS 310

[Close]

BLAST of Cp4.1LG14g06240 vs. NCBI nr
Match: gi|341657785|gb|AEK86567.1| (chloroplast beta-carotene hydroxylase [Cucurbita moschata])

HSP 1 Score: 557.8 bits (1436), Expect = 1.2e-155
Identity = 276/311 (88.75%), Postives = 294/311 (94.53%), Query Frame = 1


Query: 1   MAAALSAALGPKPLHLFLASSHLSPKPRTPFLFPPSVFRNSRIQRKIRRKTLFTVCVLVE 60
           MAA+LSA LGPKP H+FL SS+LSPKPRTP LFP SVFRNSRIQ K+RRKTL TVCVLVE
Sbjct: 1   MAASLSAVLGPKPFHVFLTSSNLSPKPRTPLLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVESLSDEEPKIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVE+L+DE+  I+VPQIPSP+VA++LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLLIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGEEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHS+K NGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKLNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 LTTTKSSINGS 312
            +T K  INGS
Sbjct: 301 -STAKKPINGS 310

[Close]

BLAST of Cp4.1LG14g06240 vs. NCBI nr
Match: gi|590673137|ref|XP_007038805.1| (Beta-hydroxylase 1, 1,B1,chy1,BCH1 isoform 1 [Theobroma cacao])

HSP 1 Score: 455.3 bits (1170), Expect = 8.6e-125
Identity = 236/307 (76.87%), Postives = 260/307 (84.69%), Query Frame = 1


Query: 1   MAAALSAALGPKPLHLFLASSHLSPKPRTPFLFPPSVFRNSRIQRKIRRKTLFTVCVLVE 60
           MAA LSAA+ PKP   FL SSHL PKP TP   P  + R  +     RRK  F VC ++E
Sbjct: 1   MAAGLSAAITPKPFRSFL-SSHLEPKP-TPLFHP--LLRLQKTLYGARRKRSFAVCFVLE 60

Query: 61  DQNSSG-EVESLSDE------EPKIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGI 120
           +Q  S  ++ +L++E      + +I++       +A++LARKRSERFTYLVAAVMSSFGI
Sbjct: 61  EQKQSAPQIVNLAEEGSEDARDSQILI----QARLAEKLARKRSERFTYLVAAVMSSFGI 120

Query: 121 TSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMH 180
           TSMAVMAVYYRF WQMEGGE+P SEMFGTF+LSVGAAVGMEFWARWAHRALWH+SLWHMH
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEVPLSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMH 180

Query: 181 ESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFV 240
           ESHH+PREGPFELNDVFAIINAVPAIALLSYGFF+KGLVPGLCFGAGLGITVFGMAYMFV
Sbjct: 181 ESHHRPREGPFELNDVFAIINAVPAIALLSYGFFNKGLVPGLCFGAGLGITVFGMAYMFV 240

Query: 241 HDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGEEELEK 300
           HDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKE+EEVGG EELEK
Sbjct: 241 HDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKEVEEVGGLEELEK 299

[Close]

BLAST of Cp4.1LG14g06240 vs. NCBI nr
Match: gi|694400895|ref|XP_009375525.1| (PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like [Pyrus x bretschneideri])

HSP 1 Score: 454.9 bits (1169), Expect = 1.1e-124
Identity = 230/307 (74.92%), Postives = 254/307 (82.74%), Query Frame = 1


Query: 1   MAAALSAALGPKPLHLFLASSHLSPKPRTPFLFPPSVFRNSRIQRKIRRKTLFTVCVLVE 60
           MA  L AA  P P  L + SSHL PKP    +F P++        + RRK  FTV V++E
Sbjct: 1   MAVGLFAATTPTPFRL-IQSSHLLPKPNPATVFFPNLRHKKSALHRTRRKLCFTVYVVME 60

Query: 61  DQ-------NSSGEVESLSDEEPKIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGI 120
           DQ       NS+G+     D +  IV+P +    VA+RL+RK+SERFTYLVAAVMSSFGI
Sbjct: 61  DQKQITHLDNSAGKAPEADDSQIPIVIPSVR---VAERLSRKKSERFTYLVAAVMSSFGI 120

Query: 121 TSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMH 180
           TSMAVMAVYYRFYWQMEGG +P SEM GTF+LSVGAAVGMEFWARWAH+ALWH+SLWHMH
Sbjct: 121 TSMAVMAVYYRFYWQMEGGNVPMSEMLGTFALSVGAAVGMEFWARWAHKALWHASLWHMH 180

Query: 181 ESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFV 240
           ESHH+PREGPFELNDVFAIINAVPAIALL+YGFFHKGLVPGLCFGAGLGITVFGMAYMFV
Sbjct: 181 ESHHRPREGPFELNDVFAIINAVPAIALLNYGFFHKGLVPGLCFGAGLGITVFGMAYMFV 240

Query: 241 HDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGEEELEK 300
           HDGLVHKRFPVGPIANVPY RKVAAAHQLHHS+KF+GVPYGLFLGPKELEEVGG EEL+K
Sbjct: 241 HDGLVHKRFPVGPIANVPYLRKVAAAHQLHHSEKFDGVPYGLFLGPKELEEVGGLEELDK 300

The following BLAST results are available for this feature:

BLAST of Cp4.1LG14g06240 vs. Swiss-Prot


Match Name	E-value	Identity	Description
BCH2_CAPAN	1.8e-122	76.90	Beta-carotene hydroxylase 2, chloroplastic (Fragment) OS=Capsicum annuum GN=CA2 ...	[more]
BCH_GENLU	2.1e-115	71.29	Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea GN=BHY PE=2 SV=1	[more]
BCH1_ARATH	8.9e-114	69.18	Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana GN=BETA-OHA...	[more]
BCH2_ARATH	1.1e-111	67.85	Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana GN=BETA-OHA...	[more]
BCH1_CAPAN	4.3e-108	64.74	Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum GN=CA1 PE=1 SV=1	[more]

BLAST of Cp4.1LG14g06240 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A088CIY4_CUCME	1.3e-159	92.18	Beta-carotene hydroxylase OS=Cucumis melo var. makuwa GN=CHXB PE=2 SV=1	[more]
A0A0A0L606_CUCSA	1.4e-158	90.68	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G183920 PE=3 SV=1	[more]
G1FQR0_CUCMO	8.6e-156	88.75	Chloroplast beta-carotene hydroxylase OS=Cucurbita moschata GN=CHYB PE=2 SV=1	[more]
A0A061G159_THECC	6.0e-125	76.87	Beta-hydroxylase 1, 1,B1,chy1,BCH1 isoform 1 OS=Theobroma cacao GN=TCM_015247 PE...	[more]
M5X1V0_PRUPE	5.1e-124	74.84	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009001mg PE=3 SV=1	[more]

BLAST of Cp4.1LG14g06240 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G25700.1	5.0e-115	69.18	beta-hydroxylase 1	[more]
AT5G52570.1	6.1e-113	67.85	beta-carotene hydroxylase 2	[more]

BLAST of Cp4.1LG14g06240 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659077599\|ref\|XP_008439286.1\|	1.8e-159	92.18	PREDICTED: beta-carotene hydroxylase 2, chloroplastic [Cucumis melo]	[more]
gi\|449445997\|ref\|XP_004140758.1\|	2.0e-158	90.68	PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like [Cucumis sativus]	[more]
gi\|341657785\|gb\|AEK86567.1\|	1.2e-155	88.75	chloroplast beta-carotene hydroxylase [Cucurbita moschata]	[more]
gi\|590673137\|ref\|XP_007038805.1\|	8.6e-125	76.87	Beta-hydroxylase 1, 1,B1,chy1,BCH1 isoform 1 [Theobroma cacao]	[more]
gi\|694400895\|ref\|XP_009375525.1\|	1.1e-124	74.92	PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like [Pyrus x bretschneide...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process
GO:0006633	fatty acid biosynthetic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity
GO:0005506	iron ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR006694	Fatty_acid_hydroxylase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006633	fatty acid biosynthetic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG14g06240.1	Cp4.1LG14g06240.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 149..276 score: 6.3
None	No IPR available	PANTHER	PTHR31899	FAMILY NOT NAMED	coord: 2..309 score: 1.1E
None	No IPR available	PANTHER	PTHR31899:SF1	BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTIC-RELATED	coord: 2..309 score: 1.1E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG14g06240	Cucsa.108110	Cucumber (Gy14) v1	cgycpeB0241
Cp4.1LG14g06240	CmoCh16G001080	Cucurbita moschata (Rifu)	cmocpeB307
Cp4.1LG14g06240	CmoCh18G012980	Cucurbita moschata (Rifu)	cmocpeB386
Cp4.1LG14g06240	Cla006149	Watermelon (97103) v1	cpewmB227
Cp4.1LG14g06240	Csa3G183920	Cucumber (Chinese Long) v2	cpecuB214
Cp4.1LG14g06240	MELO3C006938	Melon (DHL92) v3.5.1	cpemeB187
Cp4.1LG14g06240	ClCG05G008380	Watermelon (Charleston Gray)	cpewcgB207
Cp4.1LG14g06240	CSPI03G17250	Wild cucumber (PI 183967)	cpecpiB217
Cp4.1LG14g06240	Lsi05G011960	Bottle gourd (USVL1VR-Ls)	cpelsiB172
Cp4.1LG14g06240	MELO3C006938.2	Melon (DHL92) v3.6.1	cpemedB226
Cp4.1LG14g06240	CsaV3_5G002520	Cucumber (Chinese Long) v3	cpecucB0278
Cp4.1LG14g06240	CsaV3_3G017450	Cucumber (Chinese Long) v3	cpecucB0257
Cp4.1LG14g06240	BhiUN557G10	Wax gourd	cpewgoB0286
Cp4.1LG14g06240	CsGy3G017310	Cucumber (Gy14) v2	cgybcpeB327
Cp4.1LG14g06240	Carg15111	Silver-seed gourd	carcpeB1376
Cp4.1LG14g06240	Carg22126	Silver-seed gourd	carcpeB1039

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Cp4.1LG14g06240	Cp4.1LG09g00740	Cucurbita pepo (Zucchini)	cpecpeB023

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG14g06240	Silver-seed gourd	carcpeB0690
Cp4.1LG14g06240	Silver-seed gourd	carcpeB0998
Cp4.1LG14g06240	Cucumber (Chinese Long) v3	cpecucB0252
Cp4.1LG14g06240	Cucumber (Chinese Long) v3	cpecucB0275
Cp4.1LG14g06240	Cucurbita pepo (Zucchini)	cpecpeB197
Cp4.1LG14g06240	Cucurbita pepo (Zucchini)	cpecpeB232
Cp4.1LG14g06240	Cucurbita pepo (Zucchini)	cpecpeB242
Cp4.1LG14g06240	Cucurbita pepo (Zucchini)	cpecpeB245
Cp4.1LG14g06240	Cucurbita maxima (Rimu)	cmacpeB307
Cp4.1LG14g06240	Cucurbita maxima (Rimu)	cmacpeB344
Cp4.1LG14g06240	Cucurbita maxima (Rimu)	cmacpeB425
Cp4.1LG14g06240	Cucurbita maxima (Rimu)	cmacpeB608
Cp4.1LG14g06240	Cucurbita moschata (Rifu)	cmocpeB270
Cp4.1LG14g06240	Cucurbita moschata (Rifu)	cmocpeB557
Cp4.1LG14g06240	Wild cucumber (PI 183967)	cpecpiB227
Cp4.1LG14g06240	Cucumber (Chinese Long) v2	cpecuB225
Cp4.1LG14g06240	Bottle gourd (USVL1VR-Ls)	cpelsiB163
Cp4.1LG14g06240	Watermelon (Charleston Gray)	cpewcgB199
Cp4.1LG14g06240	Watermelon (97103) v1	cpewmB229
Cp4.1LG14g06240	Melon (DHL92) v3.5.1	cpemeB179
Cp4.1LG14g06240	Melon (DHL92) v3.5.1	cpemeB190
Cp4.1LG14g06240	Cucumber (Gy14) v2	cgybcpeB321
Cp4.1LG14g06240	Cucumber (Gy14) v2	cgybcpeB617
Cp4.1LG14g06240	Melon (DHL92) v3.6.1	cpemedB215
Cp4.1LG14g06240	Melon (DHL92) v3.6.1	cpemedB225
Cp4.1LG14g06240	Melon (DHL92) v3.6.1	cpemedB233