Cucsa.106530 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.106530
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Polygalacturonase-inhibiting protein 2
Location	scaffold00930 : 110613 .. 111620 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCAAATCTCAAGAAACTTTCATGCCTCCCTTTCTTCTTCCTCATCTTCTTCTTCTTCGTTACCATTTCCTCTGCGGAGCTTTGCAATCCAAATGACAAAAAAGTCCTTCTAAAAATCAAAAAAGCCTTCAACAATCCCTACATTCTCACCTCTTGGGACCCGCAAACAGACTGCTGCCATTGGTACTGTGTCAAATGCAATCGAACTACCCATCGAATCATCTCTCTCACCATCTTCGCCGACGACCGTCTCACCGGCCAAATCCCACCCGAAGTCGGCGACCTTCCATTCCTACAAACCCTTATGCTCCACAAGCTCCCAAATCTCACCGGCCCCATCCAACCCACCATAGCCAAACTACGTAACCTCGTATTCCTCGACCTCAGCTGGAACGGTCTCTCCGGCGAAATCCCAGATTCCTTGAGCACTCTAAAAAATCTCTTCATTCTTACTCTCTCATTCAACAAACTCACCGGAGAAATCCCTAGTTCCCTCTCTGAACTCCCTAACTTGGGCGGTCTCCGGTTGGATCGGAATCAATTAACAGGACAAATCCCTGAATCCTTCGGCTATTTCAGCGGAGAACAAGCCCCGGATTTGATTCTTTCACACAATCAACTTTCTGGCAAAATCCCTTCTTCTTTGTCGAGATTGAATTCCACTTCCATCGATTTGTCCCGTAACAAACTTGAAGGCGATGCGTCCATGTTCTTCGGCGCCAACAAGATGATCCAGATTCTTGATTTGTCGAGGAATTTGTTGGAGTTTAATTTGTCGGAGGTAGTGTTTCCTCAGAGTTTGACTTCGTTGGATCTTAATCATAATAAGGTGTATGGGGGTTTGCCGCCGGTGATGACGGAACTGAAATTGCAATATTTGAACGTTAGTTATAATCGACTTTGTGGTCCGATCCCGGTGGGTGGGGATATGCAGAGTTTTGATTTGTATTCTTATTTCCATAATAAGTGTTTGTGTGGAGCACCGCTTGGTGATTGCAAATGA

mRNA sequence

ATGGCTTCAAATCTCAAGAAACTTTCATGCCTCCCTttcttcttcctcatcttcttcttcttcGTTACCATTTCCTCTGCGGAGCTTTGCAATCCAAATGACAAAAAAGTCCTTCTAAAAATCAAAAAAGCCTTCAACAATCCCTACATTCTCACCTCTTGGGACCCGCAAACAGACTGCTGCCATTGGTACTGTGTCAAATGCAATCGAACTACCCATCGAATCATCTCTCTCACCATCTTCGCCGACGACCGTCTCACCGGCCAAATCCCACCCGAAGTCGGCGACCTTCCATTCCTACAAACCCTTATGCTCCACAAGCTCCCAAATCTCACCGGCCCCATCCAACCCACCATAGCCAAACTACGTAACCTCGTATTCCTCGACCTCAGCTGGAACGGTCTCTCCGGCGAAATCCCAGATTCCTTGAGCACTCTAAAAAATCTCTTCATTCTTACTCTCTCATTCAACAAACTCACCGGAGAAATCCCTAGTTCCCTCTCTGAACTCCCTAACTTGGGCGGTCTCCGGTTGGATCGGAATCAATTAACAGGACAAATCCCTGAATCCTTCGGCTATTTCAGCGGAGAACAAGCCCCGGATTTGATTCTTTCACACAATCAACTTTCTGGCAAAATCCCTTCTTCTTTGTCGAGATTGAATTCCACTTCCATCGATTTGTCCCGTAACAAACTTGAAGGCGATGCGTCCATGTTCTTCGGCGCCAACAAGATGATCCAGATTCTTGATTTGTCGAGGAATTTGTTGGAGTTTAATTTGTCGGAGGTAGTGTTTCCTCAGAGTTTGACTTCGTTGGATCTTAATCATAATAAGGTGTATGGGGGTTTGCCGCCGGTGATGACGGAACTGAAATTGCAATATTTGAACGTTAGTTATAATCGACTTTGTGGTCCGATCCCGGTGGGTGGGGATATGCAGAGTTTTGATTTGTATTCTTATTTCCATAATAAGTGTTTGTGTGGAGCACCGCTTGGTGATTGCAAATGA

Coding sequence (CDS)

ATGGCTTCAAATCTCAAGAAACTTTCATGCCTCCCTTTCTTCTTCCTCATCTTCTTCTTCTTCGTTACCATTTCCTCTGCGGAGCTTTGCAATCCAAATGACAAAAAAGTCCTTCTAAAAATCAAAAAAGCCTTCAACAATCCCTACATTCTCACCTCTTGGGACCCGCAAACAGACTGCTGCCATTGGTACTGTGTCAAATGCAATCGAACTACCCATCGAATCATCTCTCTCACCATCTTCGCCGACGACCGTCTCACCGGCCAAATCCCACCCGAAGTCGGCGACCTTCCATTCCTACAAACCCTTATGCTCCACAAGCTCCCAAATCTCACCGGCCCCATCCAACCCACCATAGCCAAACTACGTAACCTCGTATTCCTCGACCTCAGCTGGAACGGTCTCTCCGGCGAAATCCCAGATTCCTTGAGCACTCTAAAAAATCTCTTCATTCTTACTCTCTCATTCAACAAACTCACCGGAGAAATCCCTAGTTCCCTCTCTGAACTCCCTAACTTGGGCGGTCTCCGGTTGGATCGGAATCAATTAACAGGACAAATCCCTGAATCCTTCGGCTATTTCAGCGGAGAACAAGCCCCGGATTTGATTCTTTCACACAATCAACTTTCTGGCAAAATCCCTTCTTCTTTGTCGAGATTGAATTCCACTTCCATCGATTTGTCCCGTAACAAACTTGAAGGCGATGCGTCCATGTTCTTCGGCGCCAACAAGATGATCCAGATTCTTGATTTGTCGAGGAATTTGTTGGAGTTTAATTTGTCGGAGGTAGTGTTTCCTCAGAGTTTGACTTCGTTGGATCTTAATCATAATAAGGTGTATGGGGGTTTGCCGCCGGTGATGACGGAACTGAAATTGCAATATTTGAACGTTAGTTATAATCGACTTTGTGGTCCGATCCCGGTGGGTGGGGATATGCAGAGTTTTGATTTGTATTCTTATTTCCATAATAAGTGTTTGTGTGGAGCACCGCTTGGTGATTGCAAATGA

Protein sequence

MASNLKKLSCLPFFFLIFFFFVTISSAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWYCVKCNRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLRNLVFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDRNQLTGQIPESFGYFSGEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFFGANKMIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVSYNRLCGPIPVGGDMQSFDLYSYFHNKCLCGAPLGDCK*

Homology

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BLAST of Cucsa.106530 vs. Swiss-Prot
Match: PGIP_PYRCO (Polygalacturonase inhibitor OS=Pyrus communis GN=PGIP PE=1 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 7.9e-124
Identity = 222/329 (67.48%), Postives = 256/329 (77.81%), Query Frame = 1


Query: 7   KLSCLPFFFLIFFFFVTISSAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWYCV 66
           K S      L+F   +  + ++LCNP+DKKVLL+IKKAF +PY+L SW   TDCC WYCV
Sbjct: 4   KFSTFLSLTLLFSSVLNPALSDLCNPDDKKVLLQIKKAFGDPYVLASWKSDTDCCDWYCV 63

Query: 67  KCNRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLRNLV 126
            C+ TT+RI SLTIFA  +++GQIP  VGDLP+L+TL  HK PNLTGPIQP IAKL+ L 
Sbjct: 64  TCDSTTNRINSLTIFAG-QVSGQIPALVGDLPYLETLEFHKQPNLTGPIQPAIAKLKGLK 123

Query: 127 FLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDRNQLTGQ 186
            L LSW  LSG +PD LS LKNL  L LSFN LTG IPSSLSELPNLG LRLDRN+LTG 
Sbjct: 124 SLRLSWTNLSGSVPDFLSQLKNLTFLDLSFNNLTGAIPSSLSELPNLGALRLDRNKLTGH 183

Query: 187 IPESFGYFSGEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFFGANKMI 246
           IP SFG F G   PDL LSHNQLSG IP+S ++++ TSIDLSRNKLEGDAS+ FG NK  
Sbjct: 184 IPISFGQFIGN-VPDLYLSHNQLSGNIPTSFAQMDFTSIDLSRNKLEGDASVIFGLNKTT 243

Query: 247 QILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVSYNRLCGPI 306
           QI+DLSRNLLEFNLS+V FP SLTSLD+NHNK+YG +P   T+L  Q+LNVSYNRLCG I
Sbjct: 244 QIVDLSRNLLEFNLSKVEFPTSLTSLDINHNKIYGSIPVEFTQLNFQFLNVSYNRLCGQI 303

Query: 307 PVGGDMQSFDLYSYFHNKCLCGAPLGDCK 336
           PVGG +QSFD YSYFHN+CLCGAPL  CK
Sbjct: 304 PVGGKLQSFDEYSYFHNRCLCGAPLPSCK 330

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BLAST of Cucsa.106530 vs. Swiss-Prot
Match: PGIP_VITVI (Polygalacturonase inhibitor OS=Vitis vinifera GN=pgip PE=1 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 5.6e-114
Identity = 207/311 (66.56%), Postives = 238/311 (76.53%), Query Frame = 1


Query: 25  SSAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWYCVKCNRTTHRIISLTIFADD 84
           S +E CNP DKKVLL+IKKA +NPYIL SW+P TDCC WYCV+C+ TTHRI SLTIF+  
Sbjct: 25  SLSERCNPKDKKVLLQIKKALDNPYILASWNPNTDCCGWYCVECDLTTHRINSLTIFSG- 84

Query: 85  RLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLRNLVFLDLSWNGLSGEIPDSLS 144
           +L+GQIP  VGDLPFL+TL+  KL NLTG I P IAKL++L  + LSW  LSG +P   S
Sbjct: 85  QLSGQIPDAVGDLPFLETLIFRKLSNLTGQIPPAIAKLKHLKMVRLSWTNLSGPVPAFFS 144

Query: 145 TLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDRNQLTGQIPESFGYFSGEQAPDLIL 204
            LKNL  L LSFN L+G IP SLS LPNLG L LDRN LTG IP+SFG F+G   P L L
Sbjct: 145 ELKNLTYLDLSFNNLSGPIPGSLSLLPNLGALHLDRNHLTGPIPDSFGKFAGS-TPGLHL 204

Query: 205 SHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFFGANKMIQILDLSRNLLEFNLSEVV 264
           SHNQLSGKIP S    +   +DLSRNKLEGD S+FF ANK  QI+D SRNL +F+LS V 
Sbjct: 205 SHNQLSGKIPYSFRGFDPNVMDLSRNKLEGDLSIFFNANKSTQIVDFSRNLFQFDLSRVE 264

Query: 265 FPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVSYNRLCGPIPVGGDMQSFDLYSYFHNK 324
           FP+SLTSLDL+HNK+ G LP +MT L LQ+LNVSYNRLCG IPVGG +QSFD  SYFHN+
Sbjct: 265 FPKSLTSLDLSHNKIAGSLPEMMTSLDLQFLNVSYNRLCGKIPVGGKLQSFDYDSYFHNR 324

Query: 325 CLCGAPLGDCK 336
           CLCGAPL  CK
Sbjct: 325 CLCGAPLQSCK 333

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BLAST of Cucsa.106530 vs. Swiss-Prot
Match: PGIP1_ARATH (Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana GN=PGIP1 PE=2 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 2.2e-110
Identity = 202/328 (61.59%), Postives = 240/328 (73.17%), Query Frame = 1


Query: 10  CLPFFFLIFFFFVTISSAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWYCVKCN 69
           CL F   +F F  T  S +LCN NDK  LLKIKK+ NNPY L SWDPQTDCC WYC++C 
Sbjct: 8   CLLF---LFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECG 67

Query: 70  RTT--HRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLRNLVF 129
             T  HR+ +LTIF+  +++GQIP EVGDLP+L+TL+  KL NLTG IQPTIAKL+NL  
Sbjct: 68  DATVNHRVTALTIFSG-QISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRM 127

Query: 130 LDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDRNQLTGQI 189
           L LSW  L+G IPD +S LKNL  L LSFN L+G IPSSLS LP +  L L RN+LTG I
Sbjct: 128 LRLSWTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSI 187

Query: 190 PESFGYFSGEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFFGANKMIQ 249
           PESFG F G   PDL LSHNQLSG IP SL  ++   IDLSRNKL+GDASM FG+NK   
Sbjct: 188 PESFGSFPGT-VPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTW 247

Query: 250 ILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVSYNRLCGPIP 309
            +DLSRN+ +F++S+V  P++L  LDLNHN + G +P   TE  LQ+ NVSYN+LCG IP
Sbjct: 248 SIDLSRNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTEAPLQFFNVSYNKLCGHIP 307

Query: 310 VGGDMQSFDLYSYFHNKCLCGAPLGDCK 336
            GG +Q+FD YSYFHNKCLCGAPL  CK
Sbjct: 308 TGGKLQTFDSYSYFHNKCLCGAPLEICK 330

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BLAST of Cucsa.106530 vs. Swiss-Prot
Match: PGIP2_ARATH (Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana GN=PGIP2 PE=2 SV=2)

HSP 1 Score: 373.2 bits (957), Expect = 2.9e-102
Identity = 191/328 (58.23%), Postives = 234/328 (71.34%), Query Frame = 1


Query: 11  LPFFFLIFFFFVTISSA-ELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWYCVKCN 70
           +  F L+    +T S A +LC+ +DK  LLKIKK+ NNPY L SWDP+TDCC WYC++C 
Sbjct: 5   MTLFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECG 64

Query: 71  RTT--HRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLRNLVF 130
             T  HR+ SL I  D  ++GQIPPEVGDLP+L +L+  KL NLTG IQPTIAKL+NL F
Sbjct: 65  DATVNHRVTSL-IIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTF 124

Query: 131 LDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDRNQLTGQI 190
           L LSW  L+G +P+ LS LKNL  + LSFN L+G IPSSLS L  L  L L RN+LTG I
Sbjct: 125 LRLSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPI 184

Query: 191 PESFGYFSGEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFFGANKMIQ 250
           PESFG FSG+  P L LSHNQLSG IP SL   +   IDLSRNKL+GDAS+ FGA K   
Sbjct: 185 PESFGTFSGK-VPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTW 244

Query: 251 ILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVSYNRLCGPIP 310
           I+D+SRN+ +F+LS+V   ++L +LD+NHN + G +P   ++   Q LNVSYNRLCG IP
Sbjct: 245 IVDISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSKAYFQLLNVSYNRLCGRIP 304

Query: 311 VGGDMQSFDLYSYFHNKCLCGAPLGDCK 336
            G  +Q FD YS+FHNKCLCGAPL  CK
Sbjct: 305 KGEYIQRFDSYSFFHNKCLCGAPLPSCK 330

[Close]

BLAST of Cucsa.106530 vs. Swiss-Prot
Match: PGIP1_PHAVU (Polygalacturonase inhibitor 1 OS=Phaseolus vulgaris GN=PGIP1 PE=1 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.7e-73
Identity = 147/325 (45.23%), Postives = 212/325 (65.23%), Query Frame = 1


Query: 16  LIFFFFVTISSAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCH--WYCVKCNRTT- 75
           L+    +  + +ELCNP DK+ LL+IKK   NP  L+SW P TDCC+  W  V C+  T 
Sbjct: 18  LVILVSLRTALSELCNPQDKQALLQIKKDLGNPTTLSSWLPTTDCCNRTWLGVLCDTDTQ 77

Query: 76  -HRIISLTIFADDRLTGQ-IPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLRNLVFLDL 135
            +R+ +L +   +      IP  + +LP+L  L +  + NL GPI P IAKL  L +L +
Sbjct: 78  TYRVNNLDLSGHNLPKPYPIPSSLANLPYLNFLYIGGINNLVGPIPPAIAKLTQLHYLYI 137

Query: 136 SWNGLSGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDRNQLTGQIPES 195
           +   +SG IPD LS +K L  L  S+N L+G +P S+S LPNLGG+  D N+++G IP+S
Sbjct: 138 THTNVSGAIPDFLSQIKTLVTLDFSYNALSGTLPPSISSLPNLGGITFDGNRISGAIPDS 197

Query: 196 FGYFSGEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFFGANKMIQILD 255
           +G FS +    + +S N+L+GKIP + + LN   +DLSRN LEGDAS+ FG++K  + + 
Sbjct: 198 YGSFS-KLFTAMTISRNRLTGKIPPTFANLNLAFVDLSRNMLEGDASVLFGSDKNTKKIH 257

Query: 256 LSRNLLEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELK-LQYLNVSYNRLCGPIPVG 315
           L++N L F+L +V   ++L  LDL +N++YG LP  +T+LK LQ LNVS+N LCG IP G
Sbjct: 258 LAKNSLAFDLGKVGLSKNLNGLDLRNNRIYGTLPQGLTQLKFLQSLNVSFNNLCGEIPQG 317

Query: 316 GDMQSFDLYSYFHNKCLCGAPLGDC 335
           G+++ FD+ SY +NKCLCG+PL  C
Sbjct: 318 GNLKRFDVSSYANNKCLCGSPLPSC 341

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BLAST of Cucsa.106530 vs. TrEMBL
Match: K7QLL6_CUCSA (Polygalacturonase-inhibiting protein 2 OS=Cucumis sativus GN=PGIP2 PE=2 SV=1)

HSP 1 Score: 684.9 bits (1766), Expect = 5.0e-194
Identity = 335/335 (100.00%), Postives = 335/335 (100.00%), Query Frame = 1


Query: 1   MASNLKKLSCLPFFFLIFFFFVTISSAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDC 60
           MASNLKKLSCLPFFFLIFFFFVTISSAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDC
Sbjct: 1   MASNLKKLSCLPFFFLIFFFFVTISSAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDC 60

Query: 61  CHWYCVKCNRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIA 120
           CHWYCVKCNRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIA
Sbjct: 61  CHWYCVKCNRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIA 120

Query: 121 KLRNLVFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDR 180
           KLRNLVFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDR
Sbjct: 121 KLRNLVFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDR 180

Query: 181 NQLTGQIPESFGYFSGEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFF 240
           NQLTGQIPESFGYFSGEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFF
Sbjct: 181 NQLTGQIPESFGYFSGEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFF 240

Query: 241 GANKMIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVSYN 300
           GANKMIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVSYN
Sbjct: 241 GANKMIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVSYN 300

Query: 301 RLCGPIPVGGDMQSFDLYSYFHNKCLCGAPLGDCK 336
           RLCGPIPVGGDMQSFDLYSYFHNKCLCGAPLGDCK
Sbjct: 301 RLCGPIPVGGDMQSFDLYSYFHNKCLCGAPLGDCK 335

[Close]

BLAST of Cucsa.106530 vs. TrEMBL
Match: A0A0F7LFW0_LUFAC (Polygalacturonase-inhibiting protein (Fragment) OS=Luffa acutangula GN=PGIP PE=4 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 3.1e-127
Identity = 222/268 (82.84%), Postives = 242/268 (90.30%), Query Frame = 1


Query: 59  DCCHWYCVKCNRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPT 118
           DCC WYCVKC+R THRI SLTIFADDRLTGQIPP+VGDLPFL+TL+LHKLPNLTGPIQPT
Sbjct: 1   DCCDWYCVKCDRKTHRITSLTIFADDRLTGQIPPDVGDLPFLETLILHKLPNLTGPIQPT 60

Query: 119 IAKLRNLVFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRL 178
           IAKL NL FLDLSWN LSG IPD LS+L NLF+L LSFN LTG IPSSLS+LPNLGGL L
Sbjct: 61  IAKLHNLKFLDLSWNSLSGPIPDFLSSLSNLFVLKLSFNSLTGAIPSSLSQLPNLGGLEL 120

Query: 179 DRNQLTGQIPESFGYFSGEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASM 238
           DRN+L+GQIP+SFGYF GEQAP L LSHNQLSG+ PSSLSRLN TSIDL+RNKLEGDA M
Sbjct: 121 DRNKLSGQIPDSFGYFPGEQAPSLYLSHNQLSGRTPSSLSRLNFTSIDLTRNKLEGDAFM 180

Query: 239 FFGANKMIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVS 298
            FGANK +QI+DLSRNLLEFNLSEVVFPQSLTSLD+NHNKV+GGLPP MT+L  QYLNVS
Sbjct: 181 LFGANKTVQIVDLSRNLLEFNLSEVVFPQSLTSLDVNHNKVFGGLPPEMTKLNFQYLNVS 240

Query: 299 YNRLCGPIPVGGDMQSFDLYSYFHNKCL 327
           YNRLCG IPVGG++Q FD+YSYFHNKCL
Sbjct: 241 YNRLCGEIPVGGELQKFDVYSYFHNKCL 268

[Close]

BLAST of Cucsa.106530 vs. TrEMBL
Match: Q7X9Q7_CUCME (Polygalacturonase-inhibiting protein OS=Cucumis melo PE=2 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 1.5e-126
Identity = 221/320 (69.06%), Postives = 261/320 (81.56%), Query Frame = 1


Query: 16  LIFFFFVTISSAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWYCVKCNRTTHRI 75
           L+ FFF T+S AELC+PNDK+VLL IKKAFNNPYILTSW P+ DCC WYCV+C+  +HRI
Sbjct: 8   LLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDLKSHRI 67

Query: 76  ISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLRNLVFLDLSWNGL 135
           I+LTIFADD L+G IPP VGDLPFL+ LM HKLPNLTGPI PTIAKL NL +LDLSWNGL
Sbjct: 68  IALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYLDLSWNGL 127

Query: 136 SGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDRNQLTGQIPESFGYFS 195
           SG IP  L +L NL IL LSFN+ TG IPSSL+ L  LG L LDRN+LTG IP+SFG F 
Sbjct: 128 SGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPDSFGNFK 187

Query: 196 GEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFFGANKMIQILDLSRNL 255
           G + P L LSHNQLSGKIP+S+ +++   IDLSRNKL GD S+ FG+ K  +I+DLSRNL
Sbjct: 188 G-KVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNL 247

Query: 256 LEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVSYNRLCGPIPVGGDMQSF 315
           LEFN+S+VVFP++LT LDLNHNK++G +P  + +L+LQ LNVSYN LCG IP+GG +QSF
Sbjct: 248 LEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALCGRIPMGGKLQSF 307

Query: 316 DLYSYFHNKCLCGAPLGDCK 336
           D+YSYFHNKCLCG PLG CK
Sbjct: 308 DVYSYFHNKCLCGKPLGSCK 326

[Close]

BLAST of Cucsa.106530 vs. TrEMBL
Match: K7QJC6_CUCSA (Polygalacturonase-inhibiting protein 1 OS=Cucumis sativus GN=PGIP1 PE=2 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 3.4e-126
Identity = 219/320 (68.44%), Postives = 259/320 (80.94%), Query Frame = 1


Query: 16  LIFFFFVTISSAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWYCVKCNRTTHRI 75
           L+  FF T+S AELC+PNDKKVLL IKKAFNNPYILTSW P+ DCC WYCV+C+R +HRI
Sbjct: 10  LLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRKSHRI 69

Query: 76  ISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLRNLVFLDLSWNGL 135
           I+LT+FADD+L+G IPP VGDLPFL+ LM HKLPNL GPI PTIAKL NL +LDLSWNGL
Sbjct: 70  IALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLKYLDLSWNGL 129

Query: 136 SGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDRNQLTGQIPESFGYFS 195
           SG IP  L +L NL +L LSFN+ TG IPSSL+ L  LG L LDRN+LTG IPESFG F 
Sbjct: 130 SGPIPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPESFGNFK 189

Query: 196 GEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFFGANKMIQILDLSRNL 255
           G + P L LSHNQLSGKIP S+ +++   IDLSRNKL GD S+ FG+ K  +I+DLSRNL
Sbjct: 190 G-KVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNL 249

Query: 256 LEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVSYNRLCGPIPVGGDMQSF 315
           LEFN+S+VVFP++LT LDLNHNK++G +P  + +L+LQ  NVSYN LCG IP+GG +QSF
Sbjct: 250 LEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNALCGRIPMGGKLQSF 309

Query: 316 DLYSYFHNKCLCGAPLGDCK 336
           D+YSYFHNKCLCG PLG CK
Sbjct: 310 DVYSYFHNKCLCGKPLGSCK 328

[Close]

BLAST of Cucsa.106530 vs. TrEMBL
Match: A0A0A0KZN2_CUCSA (Polygalacturonase-inhibiting protein OS=Cucumis sativus GN=Csa_4G154320 PE=4 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 4.5e-126
Identity = 218/320 (68.12%), Postives = 259/320 (80.94%), Query Frame = 1


Query: 16  LIFFFFVTISSAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWYCVKCNRTTHRI 75
           L+  FF T+S AELC+PNDKKVLL IKKAFNNPYILTSW P+ DCC WYCV+C+R +HRI
Sbjct: 10  LLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRKSHRI 69

Query: 76  ISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLRNLVFLDLSWNGL 135
           I+LT+FADD+L+G IPP VGDLPFL+ LM HKLPNL GPI PTIAKL NL +LDLSWNGL
Sbjct: 70  IALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLKYLDLSWNGL 129

Query: 136 SGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDRNQLTGQIPESFGYFS 195
           SG +P  L +L NL +L LSFN+ TG IPSSL+ L  LG L LDRN+LTG IPESFG F 
Sbjct: 130 SGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPESFGNFK 189

Query: 196 GEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFFGANKMIQILDLSRNL 255
           G + P L LSHNQLSGKIP S+ +++   IDLSRNKL GD S+ FG+ K  +I+DLSRNL
Sbjct: 190 G-KVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNL 249

Query: 256 LEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVSYNRLCGPIPVGGDMQSF 315
           LEFN+S+VVFP++LT LDLNHNK++G +P  + +L+LQ  NVSYN LCG IP+GG +QSF
Sbjct: 250 LEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNALCGRIPMGGKLQSF 309

Query: 316 DLYSYFHNKCLCGAPLGDCK 336
           D+YSYFHNKCLCG PLG CK
Sbjct: 310 DVYSYFHNKCLCGKPLGSCK 328

[Close]

BLAST of Cucsa.106530 vs. TAIR10
Match: AT5G06860.1 (AT5G06860.1 polygalacturonase inhibiting protein 1)

HSP 1 Score: 400.2 bits (1027), Expect = 1.3e-111
Identity = 202/328 (61.59%), Postives = 240/328 (73.17%), Query Frame = 1


Query: 10  CLPFFFLIFFFFVTISSAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWYCVKCN 69
           CL F   +F F  T  S +LCN NDK  LLKIKK+ NNPY L SWDPQTDCC WYC++C 
Sbjct: 8   CLLF---LFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECG 67

Query: 70  RTT--HRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLRNLVF 129
             T  HR+ +LTIF+  +++GQIP EVGDLP+L+TL+  KL NLTG IQPTIAKL+NL  
Sbjct: 68  DATVNHRVTALTIFSG-QISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRM 127

Query: 130 LDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDRNQLTGQI 189
           L LSW  L+G IPD +S LKNL  L LSFN L+G IPSSLS LP +  L L RN+LTG I
Sbjct: 128 LRLSWTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSI 187

Query: 190 PESFGYFSGEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFFGANKMIQ 249
           PESFG F G   PDL LSHNQLSG IP SL  ++   IDLSRNKL+GDASM FG+NK   
Sbjct: 188 PESFGSFPGT-VPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTW 247

Query: 250 ILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVSYNRLCGPIP 309
            +DLSRN+ +F++S+V  P++L  LDLNHN + G +P   TE  LQ+ NVSYN+LCG IP
Sbjct: 248 SIDLSRNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTEAPLQFFNVSYNKLCGHIP 307

Query: 310 VGGDMQSFDLYSYFHNKCLCGAPLGDCK 336
            GG +Q+FD YSYFHNKCLCGAPL  CK
Sbjct: 308 TGGKLQTFDSYSYFHNKCLCGAPLEICK 330

[Close]

BLAST of Cucsa.106530 vs. TAIR10
Match: AT5G06870.1 (AT5G06870.1 polygalacturonase inhibiting protein 2)

HSP 1 Score: 373.2 bits (957), Expect = 1.6e-103
Identity = 191/328 (58.23%), Postives = 234/328 (71.34%), Query Frame = 1


Query: 11  LPFFFLIFFFFVTISSA-ELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWYCVKCN 70
           +  F L+    +T S A +LC+ +DK  LLKIKK+ NNPY L SWDP+TDCC WYC++C 
Sbjct: 5   MTLFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECG 64

Query: 71  RTT--HRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLRNLVF 130
             T  HR+ SL I  D  ++GQIPPEVGDLP+L +L+  KL NLTG IQPTIAKL+NL F
Sbjct: 65  DATVNHRVTSL-IIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTF 124

Query: 131 LDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDRNQLTGQI 190
           L LSW  L+G +P+ LS LKNL  + LSFN L+G IPSSLS L  L  L L RN+LTG I
Sbjct: 125 LRLSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPI 184

Query: 191 PESFGYFSGEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFFGANKMIQ 250
           PESFG FSG+  P L LSHNQLSG IP SL   +   IDLSRNKL+GDAS+ FGA K   
Sbjct: 185 PESFGTFSGK-VPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTW 244

Query: 251 ILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVSYNRLCGPIP 310
           I+D+SRN+ +F+LS+V   ++L +LD+NHN + G +P   ++   Q LNVSYNRLCG IP
Sbjct: 245 IVDISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSKAYFQLLNVSYNRLCGRIP 304

Query: 311 VGGDMQSFDLYSYFHNKCLCGAPLGDCK 336
            G  +Q FD YS+FHNKCLCGAPL  CK
Sbjct: 305 KGEYIQRFDSYSFFHNKCLCGAPLPSCK 330

[Close]

BLAST of Cucsa.106530 vs. TAIR10
Match: AT3G12145.1 (AT3G12145.1 Leucine-rich repeat (LRR) family protein)

HSP 1 Score: 303.9 bits (777), Expect = 1.2e-82
Identity = 163/325 (50.15%), Postives = 219/325 (67.38%), Query Frame = 1


Query: 11  LPFFFLIFFFFVTISSAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCH-WYCVKCN 70
           L  FF I F  +T+ S+  C  NDK  LL+IKKA  NP +L+SW+P+TDCC  W  V+C 
Sbjct: 7   LSIFFSILF--ITLPSSYSCTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVEC- 66

Query: 71  RTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLRNLVFLD 130
            T  R+  L++ + + ++GQI  ++GDL  L+TL    LP+LTG I  TI KL+NL  L 
Sbjct: 67  -TNRRVTGLSVTSGE-VSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLY 126

Query: 131 LSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDRNQLTGQIPE 190
           L    LSG IPD +S LK+L  L LSFN+ TG IP SLS++P L  ++++ N+LTG IP 
Sbjct: 127 LKHTSLSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPN 186

Query: 191 SFGYFSGEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFFGANKMIQIL 250
           SFG F G   P+L LS+N+LSGKIP SLS+ +  ++DLS N  EGDA MFFG NK    +
Sbjct: 187 SFGSFVGN-VPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVRV 246

Query: 251 DLSRNLLEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVSYNRLCGPIPVG 310
           DLSRN+  F+L +V F +S+ SLDL+ N +YG +PP +T+L L++ NVS N LCG IP G
Sbjct: 247 DLSRNMFNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKLHLEHFNVSDNHLCGKIPSG 306

Query: 311 GDMQSFDLYSYFHNKCLCGAPLGDC 335
           G +Q+F+  ++ HN CLCG PL  C
Sbjct: 307 GLLQTFEPSAFAHNICLCGTPLKAC 325

[Close]

BLAST of Cucsa.106530 vs. TAIR10
Match: AT3G12610.1 (AT3G12610.1 Leucine-rich repeat (LRR) family protein)

HSP 1 Score: 191.4 bits (485), Expect = 8.8e-49
Identity = 122/370 (32.97%), Postives = 191/370 (51.62%), Query Frame = 1


Query: 5   LKKLSCLPFFFLIFFFFVT-ISSAELCNPNDKKVLLKIKKAFNNPY--ILTSWDPQTDCC 64
           ++KL   PF  L+   F++ IS    C+P D+  L   K + + P   I  +W   TDCC
Sbjct: 1   MRKLLASPFSSLLAVVFISVISVVRCCSPKDQTALNAFKSSLSEPNLGIFNTWSENTDCC 60

Query: 65  -HWYCVKCNRTTHRIISLTIFADDR------------LTGQIPPEVGDLPFLQTLMLHKL 124
             WY + C+  + R+  +++  +              ++G I P V DL  L +L+L   
Sbjct: 61  KEWYGISCDPDSGRVTDISLRGESEDAIFQKAGRSGYMSGSIDPAVCDLTALTSLVLADW 120

Query: 125 PNLTGPIQPTIAKLRNLVFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLS 184
             +TG I P I  L +L  LDL+ N ++GEIP  +  L  L +L L+ N+++GEIP+SL+
Sbjct: 121 KGITGEIPPCITSLASLRILDLAGNKITGEIPAEIGKLSKLAVLNLAENQMSGEIPASLT 180

Query: 185 ELPNLGGLRLDRNQLTGQIPESFGYFSGEQAPDLILSHNQLSGKIPSSLSRLNS-TSIDL 244
            L  L  L L  N +TG IP  FG  S +    ++L  N+L+G IP S+S +     +DL
Sbjct: 181 SLIELKHLELTENGITGVIPADFG--SLKMLSRVLLGRNELTGSIPESISGMERLADLDL 240

Query: 245 SRNKLEGDASMFFGANKMIQIL------------------------DLSRNLLEFNLSEV 304
           S+N +EG    + G  K++ +L                        +LSRN LE  + +V
Sbjct: 241 SKNHIEGPIPEWMGNMKVLSLLNLDCNSLTGPIPGSLLSNSGLDVANLSRNALEGTIPDV 300

Query: 305 VFPQS-LTSLDLNHNKVYGGLPPVMTELK-LQYLNVSYNRLCGPIPVGGDMQSFDLYSYF 332
              ++ L SLDL+HN + G +P  ++  K + +L++S+N+LCG IP G      +  S+ 
Sbjct: 301 FGSKTYLVSLDLSHNSLSGRIPDSLSSAKFVGHLDISHNKLCGRIPTGFPFDHLEATSFS 360

[Close]

BLAST of Cucsa.106530 vs. TAIR10
Match: AT5G12940.1 (AT5G12940.1 Leucine-rich repeat (LRR) family protein)

HSP 1 Score: 167.2 bits (422), Expect = 1.8e-41
Identity = 112/359 (31.20%), Postives = 172/359 (47.91%), Query Frame = 1


Query: 17  IFFFFVTISSAELCNPNDKKVLLKIKKAFNNPYI--LTSWDPQTDCCHWYCVKCNRTTHR 76
           +  F +  ++   C P+D+  LL+ +   N PYI    +W     C  WY V C+  T R
Sbjct: 14  VVVFLLLSTTVHSCLPSDRAALLEFRAKLNEPYIGVFNTWKGLDCCKGWYGVSCDPNTRR 73

Query: 77  IISLTIFADDR------------LTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKL 136
           +  +T+  +              +TG I P +  L  L  +++     ++G I   I  L
Sbjct: 74  VAGITLRGESEDPLFQKAKRSGLMTGSISPSICKLTRLSGIIIADWKGISGVIPSCIENL 133

Query: 137 RNLVFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDRNQ 196
             L  LDL  N  SG IP ++  L  L +L L+ N L G IP S++ L +L  L L  N 
Sbjct: 134 PFLRHLDLVGNKFSGVIPANIGKLLRLKVLNLADNHLYGVIPPSITRLVSLSHLDLRNNN 193

Query: 197 LTGQIPESFGYFSGEQAPDLILSHNQLSGKIPSSLSRL-NSTSIDLSRNKLEGDASMFFG 256
           ++G IP   G    +    ++LS N++SG+IP SL+R+     ++LS N+L G     FG
Sbjct: 194 ISGVIPRDIGRL--KMVSRVLLSGNKISGQIPDSLTRIYRLADLELSMNRLTGPIPASFG 253

Query: 257 ANKMIQILDLSRNLL--------------EFNLSEVVFPQSL----------TSLDLNHN 316
              ++  L+L  NL+                NLS  +   S+          T LDL +N
Sbjct: 254 KMSVLATLNLDGNLISGMIPGSLLASSISNLNLSGNLITGSIPNTFGPRSYFTVLDLANN 313

Query: 317 KVYGGLPPVMTELK-LQYLNVSYNRLCGPIPVGGDMQSFDLYSYFHNKCLCGAPLGDCK 336
           ++ G +P  +T    + +L+VS+N LCG IP+G      D  S+ +N CLCG PLG+CK
Sbjct: 314 RLQGPIPASITAASFIGHLDVSHNHLCGKIPMGSPFDHLDATSFAYNACLCGKPLGNCK 370

[Close]

BLAST of Cucsa.106530 vs. NCBI nr
Match: gi|525507154|ref|NP_001267615.1| (polygalacturonase inhibitor-like precursor [Cucumis sativus])

HSP 1 Score: 684.9 bits (1766), Expect = 7.2e-194
Identity = 335/335 (100.00%), Postives = 335/335 (100.00%), Query Frame = 1


Query: 1   MASNLKKLSCLPFFFLIFFFFVTISSAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDC 60
           MASNLKKLSCLPFFFLIFFFFVTISSAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDC
Sbjct: 1   MASNLKKLSCLPFFFLIFFFFVTISSAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDC 60

Query: 61  CHWYCVKCNRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIA 120
           CHWYCVKCNRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIA
Sbjct: 61  CHWYCVKCNRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIA 120

Query: 121 KLRNLVFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDR 180
           KLRNLVFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDR
Sbjct: 121 KLRNLVFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDR 180

Query: 181 NQLTGQIPESFGYFSGEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFF 240
           NQLTGQIPESFGYFSGEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFF
Sbjct: 181 NQLTGQIPESFGYFSGEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFF 240

Query: 241 GANKMIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVSYN 300
           GANKMIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVSYN
Sbjct: 241 GANKMIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVSYN 300

Query: 301 RLCGPIPVGGDMQSFDLYSYFHNKCLCGAPLGDCK 336
           RLCGPIPVGGDMQSFDLYSYFHNKCLCGAPLGDCK
Sbjct: 301 RLCGPIPVGGDMQSFDLYSYFHNKCLCGAPLGDCK 335

[Close]

BLAST of Cucsa.106530 vs. NCBI nr
Match: gi|659102348|ref|XP_008452081.1| (PREDICTED: polygalacturonase inhibitor-like [Cucumis melo])

HSP 1 Score: 645.2 bits (1663), Expect = 6.3e-182
Identity = 316/337 (93.77%), Postives = 326/337 (96.74%), Query Frame = 1


Query: 1   MASNLKKLSCLPFFFLIFFFF--VTISSAELCNPNDKKVLLKIKKAFNNPYILTSWDPQT 60
           MASNL KLS LPFFF+ FFFF  +TIS AELCNPNDKKVLLKIKKAFNNPYILTSWDPQT
Sbjct: 1   MASNLNKLSWLPFFFIFFFFFFFITISFAELCNPNDKKVLLKIKKAFNNPYILTSWDPQT 60

Query: 61  DCCHWYCVKCNRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPT 120
           DCCHWYCVKC+RTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPT
Sbjct: 61  DCCHWYCVKCDRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPT 120

Query: 121 IAKLRNLVFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRL 180
           IAKL NL FLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTG+IPSSLSELP+LGGLRL
Sbjct: 121 IAKLHNLKFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGKIPSSLSELPSLGGLRL 180

Query: 181 DRNQLTGQIPESFGYFSGEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASM 240
           DRNQLTGQIP+SFGYF+GEQAPDLILSHNQLSGKIPSSLSRLN TSIDLSRNKLEGDA M
Sbjct: 181 DRNQLTGQIPDSFGYFAGEQAPDLILSHNQLSGKIPSSLSRLNFTSIDLSRNKLEGDAFM 240

Query: 241 FFGANKMIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVS 300
           FFGANK IQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKV+GGLPPVMTELKLQYLNVS
Sbjct: 241 FFGANKTIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLPPVMTELKLQYLNVS 300

Query: 301 YNRLCGPIPVGGDMQSFDLYSYFHNKCLCGAPLGDCK 336
           YNRLCGPIPVGG++QSFDLYSYFHNKCLCGAPLGDCK
Sbjct: 301 YNRLCGPIPVGGNLQSFDLYSYFHNKCLCGAPLGDCK 337

[Close]

BLAST of Cucsa.106530 vs. NCBI nr
Match: gi|821683977|gb|AKH61014.1| (polygalacturonase-inhibiting protein, partial [Luffa acutangula])

HSP 1 Score: 463.0 bits (1190), Expect = 4.5e-127
Identity = 222/268 (82.84%), Postives = 242/268 (90.30%), Query Frame = 1


Query: 59  DCCHWYCVKCNRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPT 118
           DCC WYCVKC+R THRI SLTIFADDRLTGQIPP+VGDLPFL+TL+LHKLPNLTGPIQPT
Sbjct: 1   DCCDWYCVKCDRKTHRITSLTIFADDRLTGQIPPDVGDLPFLETLILHKLPNLTGPIQPT 60

Query: 119 IAKLRNLVFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRL 178
           IAKL NL FLDLSWN LSG IPD LS+L NLF+L LSFN LTG IPSSLS+LPNLGGL L
Sbjct: 61  IAKLHNLKFLDLSWNSLSGPIPDFLSSLSNLFVLKLSFNSLTGAIPSSLSQLPNLGGLEL 120

Query: 179 DRNQLTGQIPESFGYFSGEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASM 238
           DRN+L+GQIP+SFGYF GEQAP L LSHNQLSG+ PSSLSRLN TSIDL+RNKLEGDA M
Sbjct: 121 DRNKLSGQIPDSFGYFPGEQAPSLYLSHNQLSGRTPSSLSRLNFTSIDLTRNKLEGDAFM 180

Query: 239 FFGANKMIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVS 298
            FGANK +QI+DLSRNLLEFNLSEVVFPQSLTSLD+NHNKV+GGLPP MT+L  QYLNVS
Sbjct: 181 LFGANKTVQIVDLSRNLLEFNLSEVVFPQSLTSLDVNHNKVFGGLPPEMTKLNFQYLNVS 240

Query: 299 YNRLCGPIPVGGDMQSFDLYSYFHNKCL 327
           YNRLCG IPVGG++Q FD+YSYFHNKCL
Sbjct: 241 YNRLCGEIPVGGELQKFDVYSYFHNKCL 268

[Close]

BLAST of Cucsa.106530 vs. NCBI nr
Match: gi|940373614|ref|NP_001303611.1| (polygalacturonase inhibitor-like precursor [Cucumis melo])

HSP 1 Score: 460.7 bits (1184), Expect = 2.2e-126
Identity = 221/320 (69.06%), Postives = 261/320 (81.56%), Query Frame = 1


Query: 16  LIFFFFVTISSAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWYCVKCNRTTHRI 75
           L+ FFF T+S AELC+PNDK+VLL IKKAFNNPYILTSW P+ DCC WYCV+C+  +HRI
Sbjct: 8   LLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDLKSHRI 67

Query: 76  ISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLRNLVFLDLSWNGL 135
           I+LTIFADD L+G IPP VGDLPFL+ LM HKLPNLTGPI PTIAKL NL +LDLSWNGL
Sbjct: 68  IALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYLDLSWNGL 127

Query: 136 SGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDRNQLTGQIPESFGYFS 195
           SG IP  L +L NL IL LSFN+ TG IPSSL+ L  LG L LDRN+LTG IP+SFG F 
Sbjct: 128 SGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPDSFGNFK 187

Query: 196 GEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFFGANKMIQILDLSRNL 255
           G + P L LSHNQLSGKIP+S+ +++   IDLSRNKL GD S+ FG+ K  +I+DLSRNL
Sbjct: 188 G-KVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNL 247

Query: 256 LEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVSYNRLCGPIPVGGDMQSF 315
           LEFN+S+VVFP++LT LDLNHNK++G +P  + +L+LQ LNVSYN LCG IP+GG +QSF
Sbjct: 248 LEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALCGRIPMGGKLQSF 307

Query: 316 DLYSYFHNKCLCGAPLGDCK 336
           D+YSYFHNKCLCG PLG CK
Sbjct: 308 DVYSYFHNKCLCGKPLGSCK 326

[Close]

BLAST of Cucsa.106530 vs. NCBI nr
Match: gi|821595287|ref|NP_001295863.1| (polygalacturonase inhibitor-like precursor [Cucumis sativus])

HSP 1 Score: 459.5 bits (1181), Expect = 4.9e-126
Identity = 219/320 (68.44%), Postives = 259/320 (80.94%), Query Frame = 1


Query: 16  LIFFFFVTISSAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWYCVKCNRTTHRI 75
           L+  FF T+S AELC+PNDKKVLL IKKAFNNPYILTSW P+ DCC WYCV+C+R +HRI
Sbjct: 10  LLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRKSHRI 69

Query: 76  ISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLRNLVFLDLSWNGL 135
           I+LT+FADD+L+G IPP VGDLPFL+ LM HKLPNL GPI PTIAKL NL +LDLSWNGL
Sbjct: 70  IALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLKYLDLSWNGL 129

Query: 136 SGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDRNQLTGQIPESFGYFS 195
           SG IP  L +L NL +L LSFN+ TG IPSSL+ L  LG L LDRN+LTG IPESFG F 
Sbjct: 130 SGPIPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPESFGNFK 189

Query: 196 GEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFFGANKMIQILDLSRNL 255
           G + P L LSHNQLSGKIP S+ +++   IDLSRNKL GD S+ FG+ K  +I+DLSRNL
Sbjct: 190 G-KVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNL 249

Query: 256 LEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVSYNRLCGPIPVGGDMQSF 315
           LEFN+S+VVFP++LT LDLNHNK++G +P  + +L+LQ  NVSYN LCG IP+GG +QSF
Sbjct: 250 LEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNALCGRIPMGGKLQSF 309

Query: 316 DLYSYFHNKCLCGAPLGDCK 336
           D+YSYFHNKCLCG PLG CK
Sbjct: 310 DVYSYFHNKCLCGKPLGSCK 328

The following BLAST results are available for this feature:

BLAST of Cucsa.106530 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PGIP_PYRCO	7.9e-124	67.48	Polygalacturonase inhibitor OS=Pyrus communis GN=PGIP PE=1 SV=1	[more]
PGIP_VITVI	5.6e-114	66.56	Polygalacturonase inhibitor OS=Vitis vinifera GN=pgip PE=1 SV=1	[more]
PGIP1_ARATH	2.2e-110	61.59	Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana GN=PGIP1 PE=2 SV=1	[more]
PGIP2_ARATH	2.9e-102	58.23	Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana GN=PGIP2 PE=2 SV=2	[more]
PGIP1_PHAVU	1.7e-73	45.23	Polygalacturonase inhibitor 1 OS=Phaseolus vulgaris GN=PGIP1 PE=1 SV=1	[more]

BLAST of Cucsa.106530 vs. TrEMBL


Match Name	E-value	Identity	Description
K7QLL6_CUCSA	5.0e-194	100.00	Polygalacturonase-inhibiting protein 2 OS=Cucumis sativus GN=PGIP2 PE=2 SV=1	[more]
A0A0F7LFW0_LUFAC	3.1e-127	82.84	Polygalacturonase-inhibiting protein (Fragment) OS=Luffa acutangula GN=PGIP PE=4...	[more]
Q7X9Q7_CUCME	1.5e-126	69.06	Polygalacturonase-inhibiting protein OS=Cucumis melo PE=2 SV=1	[more]
K7QJC6_CUCSA	3.4e-126	68.44	Polygalacturonase-inhibiting protein 1 OS=Cucumis sativus GN=PGIP1 PE=2 SV=1	[more]
A0A0A0KZN2_CUCSA	4.5e-126	68.13	Polygalacturonase-inhibiting protein OS=Cucumis sativus GN=Csa_4G154320 PE=4 SV=...	[more]

BLAST of Cucsa.106530 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G06860.1	1.3e-111	61.59	polygalacturonase inhibiting protein 1	[more]
AT5G06870.1	1.6e-103	58.23	polygalacturonase inhibiting protein 2	[more]
AT3G12145.1	1.2e-82	50.15	Leucine-rich repeat (LRR) family protein	[more]
AT3G12610.1	8.8e-49	32.97	Leucine-rich repeat (LRR) family protein	[more]
AT5G12940.1	1.8e-41	31.20	Leucine-rich repeat (LRR) family protein	[more]

BLAST of Cucsa.106530 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|525507154\|ref\|NP_001267615.1\|	7.2e-194	100.00	polygalacturonase inhibitor-like precursor [Cucumis sativus]	[more]
gi\|659102348\|ref\|XP_008452081.1\|	6.3e-182	93.77	PREDICTED: polygalacturonase inhibitor-like [Cucumis melo]	[more]
gi\|821683977\|gb\|AKH61014.1\|	4.5e-127	82.84	polygalacturonase-inhibiting protein, partial [Luffa acutangula]	[more]
gi\|940373614\|ref\|NP_001303611.1\|	2.2e-126	69.06	polygalacturonase inhibitor-like precursor [Cucumis melo]	[more]
gi\|821595287\|ref\|NP_001295863.1\|	4.9e-126	68.44	polygalacturonase inhibitor-like precursor [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001611	Leu-rich_rpt
IPR013210	LRR_N_plant-typ

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0048046	apoplast
cellular_component	GO:0005618	cell wall
molecular_function	GO:0005515	protein binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.106530.1	Cucsa.106530.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001611	Leucine-rich repeat	PFAM	PF00560	LRR_1	coord: 291..306 score: 1.0coord: 124..145 score:
IPR001611	Leucine-rich repeat	PFAM	PF13516	LRR_6	coord: 267..278 score:
IPR013210	Leucine-rich repeat-containing N-terminal, plant-type	PFAM	PF08263	LRRNT_2	coord: 32..69 score: 6.0
None	No IPR available	PANTHER	PTHR27000	FAMILY NOT NAMED	coord: 11..330 score: 1.7E
None	No IPR available	PANTHER	PTHR27000:SF136	POLYGALACTURONASE INHIBITOR 1-RELATED	coord: 11..330 score: 1.7E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.106530	Csa4G046710	Cucumber (Chinese Long) v2	cgycuB129
Cucsa.106530	MELO3C016384	Melon (DHL92) v3.5.1	cgymeB141
Cucsa.106530	CSPI04G05490	Wild cucumber (PI 183967)	cgycpiB130
Cucsa.106530	MELO3C016384.2	Melon (DHL92) v3.6.1	cgymedB141
Cucsa.106530	CsaV3_4G005640	Cucumber (Chinese Long) v3	cgycucB134

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.106530	Silver-seed gourd	carcgyB1059
Cucsa.106530	Silver-seed gourd	carcgyB1133
Cucsa.106530	Watermelon (97103) v2	cgywmbB146
Cucsa.106530	Wax gourd	cgywgoB165
Cucsa.106530	Wax gourd	cgywgoB167
Cucsa.106530	Cucurbita maxima (Rimu)	cgycmaB0246
Cucsa.106530	Cucurbita maxima (Rimu)	cgycmaB0245
Cucsa.106530	Cucurbita moschata (Rifu)	cgycmoB0244
Cucsa.106530	Cucurbita moschata (Rifu)	cgycmoB0245
Cucsa.106530	Watermelon (Charleston Gray)	cgywcgB151
Cucsa.106530	Watermelon (97103) v1	cgywmB147
Cucsa.106530	Cucurbita pepo (Zucchini)	cgycpeB0237
Cucsa.106530	Cucurbita pepo (Zucchini)	cgycpeB0238
Cucsa.106530	Bottle gourd (USVL1VR-Ls)	cgylsiB140