Cucsa.104600 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.104600 vs. Swiss-Prot
Match: ASO_CUCSA (L-ascorbate oxidase OS=Cucumis sativus PE=1 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 1.5e-172
Identity = 288/542 (53.14%), Postives = 370/542 (68.27%), Query Frame = 1

Query: 12  NYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTENVAIHWHGI 71
           +YKW+V+    SPDC + + + ING  PGPTI A+ GD V+VEL N L TE V IHWHGI
Sbjct: 40  HYKWDVEYMFWSPDCVENIVMGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGI 99

Query: 72  RQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWVP 131
            Q GTPW+DGT  ++QC I PGETF Y+FVVD+ GTY YH H GMQR  GLYGS++V  P
Sbjct: 100 LQRGTPWADGTASISQCAINPGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPP 159

Query: 132 EGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQGRGRFNCSI 191
           EG++EPF YD + NL+L+DW+H+S H+   GL++      W+GEP S+LI G+G+F+CSI
Sbjct: 160 EGRSEPFHYDEEINLLLSDWWHQSVHKQEVGLSS--KPMRWIGEPQSILINGKGQFDCSI 219

Query: 192 LGSSSG---TCNSSSTE-CVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEAD 251
               +     C  S  E C    L V P KTYR+R++S T+L++L+F I  H + VVEAD
Sbjct: 220 AAKYNQGLKQCELSGKEKCAPFILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEAD 279

Query: 252 GHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNYYPNH 311
           G+YV+PF  +++ IYSGE+YSVLI  DQ+P  NYW++  V +R   TPPGL + NY PN 
Sbjct: 280 GNYVQPFVTSDIDIYSGESYSVLITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNS 339

Query: 312 QSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQNTVNGRRR 371
            S+LP + PP  P W D       +  I A  G    PP   ++ + LLNTQN +NG  +
Sbjct: 340 ASKLPISPPPETPHWEDFDRSKNFTFRIFAAMGS-PKPPVRYNRRLFLLNTQNRINGFMK 399

Query: 372 WSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIYR 431
           W++NNVS  +P TPYL A+K  L   F+Q  PP  +   NYDI NP PNPE T  + +Y+
Sbjct: 400 WAINNVSLALPPTPYLAAMKMRLNTAFNQNPPPETFPL-NYDINNPPPNPETTTGNGVYK 459

Query: 432 LDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYNLENPIM 491
            +   TVD+ILQNANM++PN SE HPWHLHGHDFWVLGYGEGKF   +D  K NL+NP +
Sbjct: 460 FNMGETVDVILQNANMLNPNMSEIHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPL 519

Query: 492 KNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSIMGCG 550
           +NTV + PYGWTA+RFVADNPGVWAFHCHI+ H  MGMGVVF EG+  VG +P   + CG
Sbjct: 520 RNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACG 577

BLAST of Cucsa.104600 vs. Swiss-Prot
Match: ASO_CUCMA (L-ascorbate oxidase OS=Cucurbita maxima GN=AAO PE=1 SV=2)

HSP 1 Score: 603.2 bits (1554), Expect = 2.9e-171
Identity = 285/550 (51.82%), Postives = 377/550 (68.55%), Query Frame = 1

Query: 8   SRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTENVAIH 67
           S+  +YKWEV+    +PDC + + + ING+ PGPTI A+ GDTV+VEL N L TE V IH
Sbjct: 31  SQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIRANAGDTVVVELINKLHTEGVVIH 90

Query: 68  WHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIV 127
           WHGI Q GTPW+DGT  ++QC I PGETF Y F VD PGT+ YH H GMQR  GLYGS++
Sbjct: 91  WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 150

Query: 128 VWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQGRGRF 187
           V  P+G+ EPF YD + NL+L+DW+H+S H+   GL++      W+GEP ++L+ GRG+F
Sbjct: 151 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSS--KPIRWIGEPQTILLNGRGQF 210

Query: 188 NCSILG---SSSGTCNSSSTE-CVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTV 247
           +CSI     S+   C    +E C  +   V+P KTYR+R++S T+L+AL+F I  H + V
Sbjct: 211 DCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMPKKTYRIRIASTTALAALNFAIGNHPLLV 270

Query: 248 VEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNY 307
           VEADG+YV+PF  +++ IYSGE+YSVLI  DQ+PS NYW++     R   TPPGL + NY
Sbjct: 271 VEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRGRHPNTPPGLTLLNY 330

Query: 308 YPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQNTVN 367
            PN  S+LP + PP  PAW+D       +  I A  G    PP  +++ I LLNTQN +N
Sbjct: 331 LPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITAAMG-SPKPPVKSNRRIFLLNTQNVIN 390

Query: 368 GRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSD 427
           G  +W++N+VS  +P TPYL A+K NLLH F Q  PP  +  ++YDI  P  N +  + +
Sbjct: 391 GYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNPPPEVFP-EDYDIDTPPTNEKTKIGN 450

Query: 428 AIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYNLE 487
            +Y+      VD+ILQNANMM  N SE HPWHLHGHDFWVLGYG+GKF   ++ S  NL+
Sbjct: 451 GVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVLGYGDGKFTA-EEESSLNLK 510

Query: 488 NPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSI 547
           NP ++NTV + PYGWTA+RFVADNPGVWAFHCHI+ H  MGMGVVF EG+E+VG++PT  
Sbjct: 511 NPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKA 570

Query: 548 MGCGESKRFL 554
           + CG + + L
Sbjct: 571 LACGGTAKSL 575

BLAST of Cucsa.104600 vs. Swiss-Prot
Match: ASO_CUCPM (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo PE=1 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 3.7e-171
Identity = 284/550 (51.64%), Postives = 378/550 (68.73%), Query Frame = 1

Query: 8   SRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTENVAIH 67
           S+  +YKWEV+    +P+C + + + ING+ PGPTI A+ GD+V+VEL N L TE V IH
Sbjct: 1   SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIH 60

Query: 68  WHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIV 127
           WHGI Q GTPW+DGT  ++QC I PGETF Y F VD PGT+ YH H GMQR  GLYGS++
Sbjct: 61  WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 120

Query: 128 VWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQGRGRF 187
           V  P+G+ EPF YD + NL+L+DW+H+S H+   GL++      W+GEP ++L+ GRG+F
Sbjct: 121 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSS--KPIRWIGEPQTILLNGRGQF 180

Query: 188 NCSILG---SSSGTCNSSSTE-CVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTV 247
           +CSI     S+   C    +E C  +   V P KTYR+R++S T+L+AL+F I  H + V
Sbjct: 181 DCSIAAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLV 240

Query: 248 VEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNY 307
           VEADG+YV+PF  +++ IYSGE+YSVLI  DQ+PS NYW++    +R   TPPGL + NY
Sbjct: 241 VEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNY 300

Query: 308 YPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQNTVN 367
            PN  S+LP + PP  PAW+D       +  I A  G    PP   ++ I LLNTQN +N
Sbjct: 301 LPNSVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMG-SPKPPVKFNRRIFLLNTQNVIN 360

Query: 368 GRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSD 427
           G  +W++N+VS  +P TPYL A+K+NLLH F Q  PP  +  ++YDI  P  N +  + +
Sbjct: 361 GYVKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFP-EDYDIDTPPTNEKTRIGN 420

Query: 428 AIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYNLE 487
            +Y+      VD+ILQNANMM  N SETHPWHLHGHDFWVLGYG+GKF   ++ S  NL+
Sbjct: 421 GVYQFKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSA-EEESSLNLK 480

Query: 488 NPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSI 547
           NP ++NTV + PYGWTA+RFVADNPGVWAFHCHI+ H  MGMGVVF EG+E+VG++PT  
Sbjct: 481 NPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKA 540

Query: 548 MGCGESKRFL 554
           + CG + + L
Sbjct: 541 LACGGTAKSL 545

BLAST of Cucsa.104600 vs. Swiss-Prot
Match: ASO_TOBAC (L-ascorbate oxidase OS=Nicotiana tabacum GN=AAO PE=2 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 4.7e-166
Identity = 285/554 (51.44%), Postives = 370/554 (66.79%), Query Frame = 1

Query: 8   SRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTENVAIH 67
           ++T ++KW+V+    SPD  + V + ING+ PGPTI A  GDTV V L N L TE V IH
Sbjct: 28  AKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTIRAKAGDTVAVHLTNKLHTEGVVIH 87

Query: 68  WHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIV 127
           WHGIRQIGTPW+DGT  ++QC I PGETF Y+F VD+ GTY YH HYGMQR  GLYGS++
Sbjct: 88  WHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVDKAGTYFYHGHYGMQRSAGLYGSLI 147

Query: 128 VWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQGRGRF 187
           V V EG+ EPF YD + NL+L+DW+HK SHE    L++  +   W+GEP +LL+ GRG++
Sbjct: 148 VEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLSS--NPLRWIGEPQTLLLNGRGQY 207

Query: 188 NCSILGSSSGTCNSSSTECVLHP--------LTVVPGKTYRLRVSSLTSLSALSFQIEGH 247
           NCS+    S        +C L          L V P K YRLRV+S T+L +LS  I GH
Sbjct: 208 NCSLAARFS---KPPLPQCKLRGGEQYAPQILRVRPNKIYRLRVASTTALGSLSLAIGGH 267

Query: 248 TMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLA 307
            M VVEADG+YV+PF V ++ IYSGE+YSVL K DQDP++NYWI+ NV  R   TP GL 
Sbjct: 268 KMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKNYWISINVRGREPKTPQGLT 327

Query: 308 IFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQ 367
           + NY PN  S+ P   PP  P WND     + S  I A  G    PP+   + I+LLNTQ
Sbjct: 328 LLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALMGSPKPPPQ-NHRRIILLNTQ 387

Query: 368 NTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEA 427
           N ++G  +W++NNVS  +P   YL ++++  ++ F  + PP  +  K+YD+   APN  +
Sbjct: 388 NKIDGYTKWAINNVSLVLPTQLYLGSIRYG-INAFDTKPPPDNFP-KDYDVLKQAPNSNS 447

Query: 428 TVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSK 487
           T  + +Y L FN+T+DIILQNAN ++ + SE HPWHLHGHDFWVLGYGEGKF   KD  K
Sbjct: 448 TYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHDFWVLGYGEGKFS-EKDVKK 507

Query: 488 YNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKL 547
           +NL+NP ++NT  + P+GWTALRFV DNPGVWAFHCHI+ H  MGMGV+F EG+  V K+
Sbjct: 508 FNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPHLHMGMGVIFAEGVHLVKKI 567

Query: 548 PTSIMGCGESKRFL 554
           P   + CG + + L
Sbjct: 568 PKEALACGLTGKML 572

BLAST of Cucsa.104600 vs. Swiss-Prot
Match: LAC22_ORYSJ (Laccase-22 OS=Oryza sativa subsp. japonica GN=LAC22 PE=2 SV=2)

HSP 1 Score: 276.2 bits (705), Expect = 8.0e-73
Identity = 176/533 (33.02%), Postives = 274/533 (51.41%), Query Frame = 1

Query: 10  TLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTENVAIHWH 69
           T +YK+ V     +  C  K  LT+NG+ PGPT+YA +GD V+V++ N +   NV IHWH
Sbjct: 30  TRHYKFNVVMRNMTRLCSTKPILTVNGKFPGPTLYAREGDNVLVKVVNHV-AHNVTIHWH 89

Query: 70  GIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVD-RPGTYLYHAHYGMQREDGLYGSIVV 129
           G+RQI T W DG   +TQCPI PG +F Y F +  + GT L+HAH    R   ++G+IV+
Sbjct: 90  GVRQIRTGWYDGPAYITQCPIQPGSSFLYNFTITGQRGTLLWHAHINWLRAT-VHGAIVI 149

Query: 130 WVPEGQTEPF-SYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQGRGRF 189
               G   PF +   +  ++L +W+ + +          G G N     DS  I G    
Sbjct: 150 LPKLGVPYPFPAPHKEAVIVLGEWWKEDTETVINQAMQLGVGPNI---SDSHTINGHPGP 209

Query: 190 NCSILGSSSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEAD 249
                 S  G             L+V  GKTY LR+ +      L F++ GH +TVVE D
Sbjct: 210 LSECASSQDG-----------FKLSVENGKTYMLRIINAALNDDLFFKVAGHELTVVEVD 269

Query: 250 GHYVEPFQVNNLFIYSGETYSVLIKADQDPSR-----NYWITTNVVSRSRTTPPGLAIFN 309
             Y +PF+ + L I  G+T +VL++A+Q   R     + ++   V   ++T    L   N
Sbjct: 270 AVYTKPFKTDTLLITPGQTTNVLVRANQGAGRYLLSVSPFMDAPVQVDNKTGTATLHYAN 329

Query: 310 YYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNT---- 369
              +  + L    PP   A + V+       ++ +++   + P      +++ +      
Sbjct: 330 TVSSSMASLTLVKPPPQNATHIVSKFTDSLHSLNSKEYPANVPQTVDHSLLLTVGVGVNP 389

Query: 370 -QNTVNGRRR-WSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPN 429
             + +NG R   ++NNV+F MP TP L A  +N+   F+++ P T     NY    P  N
Sbjct: 390 CPSCINGTRVVGTINNVTFIMPSTPILQAHYYNIPGVFTEDFPATPLHKFNYTGSGPK-N 449

Query: 430 PEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKD 489
            +      +YRL +N++V ++LQ+  ++SP   E+HP HLHG +F+V+G G G ++    
Sbjct: 450 LQTMNGTRVYRLPYNASVQVVLQDTGIISP---ESHPIHLHGFNFFVVGKGVGNYNPRTS 509

Query: 490 PSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVF 530
           PS +NL +PI +NT+ +   GWTA+RF +DNPGVW  HCH + H   G+ + F
Sbjct: 510 PSTFNLIDPIERNTIGVPTGGWTAIRFRSDNPGVWFMHCHFEVHTSWGLKMAF 542

BLAST of Cucsa.104600 vs. TrEMBL
Match: A0A0A0KK62_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G500640 PE=3 SV=1)

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 549/555 (98.92%), Postives = 551/555 (99.28%), Query Frame = 1

Query: 1   MASPLQKSRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLF 60
           + S   +SRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLF
Sbjct: 22  LQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLF 81

Query: 61  TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQRED 120
           TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQRED
Sbjct: 82  TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQRED 141

Query: 121 GLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLL 180
           GLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLL
Sbjct: 142 GLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLL 201

Query: 181 IQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHT 240
           IQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHT
Sbjct: 202 IQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHT 261

Query: 241 MTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAI 300
           MTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAI
Sbjct: 262 MTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAI 321

Query: 301 FNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQN 360
           FNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQN
Sbjct: 322 FNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQN 381

Query: 361 TVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEAT 420
           TVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEAT
Sbjct: 382 TVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEAT 441

Query: 421 VSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKY 480
           VSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKY
Sbjct: 442 VSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKY 501

Query: 481 NLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLP 540
           NLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLP
Sbjct: 502 NLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLP 561

Query: 541 TSIMGCGESKRFLRP 556
           TSIMGCGESKRFLRP
Sbjct: 562 TSIMGCGESKRFLRP 576

BLAST of Cucsa.104600 vs. TrEMBL
Match: U5G9V4_POPTR (L-ascorbate oxidase family protein OS=Populus trichocarpa GN=POPTR_0007s05890g PE=3 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 4.0e-257
Identity = 417/554 (75.27%), Postives = 470/554 (84.84%), Query Frame = 1

Query: 4   PLQKSRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTEN 63
           P+  +R  +YKWE+K E KSPDC+KK+ +TINGR+PGPTI A Q DTVIVE+KNSL TEN
Sbjct: 17  PIADARIRHYKWELKYEYKSPDCYKKLVITINGRTPGPTILAKQNDTVIVEVKNSLLTEN 76

Query: 64  VAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLY 123
            AIHWHGIRQIGTPW DGTEGVTQCPILPG+TF Y+FVVDRPGTYLYHAHYGMQRE G+Y
Sbjct: 77  TAIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFVYKFVVDRPGTYLYHAHYGMQREAGIY 136

Query: 124 GSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQG 183
           GSI V +P+G++EPF+YD DR++ILTDWYHKS++E A GL++    F WVGEP SLLIQG
Sbjct: 137 GSIRVALPDGESEPFAYDYDRSIILTDWYHKSTYEQAAGLSS--IPFQWVGEPQSLLIQG 196

Query: 184 RGRFNCSILGS--SSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTM 243
           +GRF+CS       +  CN+++ EC L+  TVVPGKTYRLR+SSL++LSALSFQIEGH M
Sbjct: 197 KGRFDCSAANPPLKADVCNNTNPECSLYSTTVVPGKTYRLRISSLSALSALSFQIEGHNM 256

Query: 244 TVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIF 303
           TVVEADGHYVEPF V NLFIYSGETYSVL+K DQDPSRNYW TTNVVSR+ TTPPGLAIF
Sbjct: 257 TVVEADGHYVEPFVVKNLFIYSGETYSVLVKTDQDPSRNYWATTNVVSRNATTPPGLAIF 316

Query: 304 NYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQNT 363
           NYYPNH  R PPT PP+ P WND+ PR  QS AIKAR+G I+ PP  +D+VIVLLNTQNT
Sbjct: 317 NYYPNHPRRSPPTIPPSGPMWNDIAPRFNQSVAIKARRGHIYPPPATSDRVIVLLNTQNT 376

Query: 364 VNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATV 423
           VNG  RWS+N VSFN+PHTPYLIALK NLLHTFSQ  PP GYDFKNYDIF    N  AT 
Sbjct: 377 VNGNVRWSVNKVSFNIPHTPYLIALKENLLHTFSQTPPPEGYDFKNYDIFARQNNTNATT 436

Query: 424 SDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYN 483
           SDAIYRL  NSTVDIILQNAN M+PNNSETHPWHLHGHDFWVLGYG+GKFD   DP  YN
Sbjct: 437 SDAIYRLQLNSTVDIILQNANTMNPNNSETHPWHLHGHDFWVLGYGKGKFDPINDPKNYN 496

Query: 484 LENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPT 543
           L +PIMKNTVP+HP+GWTALRF ADNPGVWAFHCHI++HFFMGMGVVFEEGIERVGKLP+
Sbjct: 497 LVDPIMKNTVPVHPFGWTALRFKADNPGVWAFHCHIESHFFMGMGVVFEEGIERVGKLPS 556

Query: 544 SIMGCGESKRFLRP 556
           SIMGCGE+KR L+P
Sbjct: 557 SIMGCGETKRLLKP 568

BLAST of Cucsa.104600 vs. TrEMBL
Match: B9HFP2_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0007s05910g PE=3 SV=2)

HSP 1 Score: 891.7 bits (2303), Expect = 4.5e-256
Identity = 415/554 (74.91%), Postives = 471/554 (85.02%), Query Frame = 1

Query: 4   PLQKSRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTEN 63
           P+ ++R  +YK+E+K E KSPDC+KK+ +TINGR+PGPTI+A Q DTVIVE+KNSL TEN
Sbjct: 31  PIAEARIRHYKFELKYEYKSPDCYKKLVITINGRTPGPTIFAQQNDTVIVEVKNSLLTEN 90

Query: 64  VAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLY 123
            AIHWHGIRQIGTPW DGTEGVTQCPILPG+TF Y+FVVDRPGTYLYHAHYGMQRE G+Y
Sbjct: 91  TAIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFVYKFVVDRPGTYLYHAHYGMQREAGIY 150

Query: 124 GSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQG 183
           GSI V +P+G++EPF+YD DR++ILTDWYHKS++E ATGL++    F WVGEP SLLIQG
Sbjct: 151 GSIRVALPDGESEPFAYDYDRSIILTDWYHKSTYEQATGLSS--IPFQWVGEPQSLLIQG 210

Query: 184 RGRFNCSILGS--SSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTM 243
           +GRF+CS       +  CN++S EC L+  TVVPGKTYRLR+SSLT+LSALSFQIEGH++
Sbjct: 211 KGRFDCSAANPPLKADVCNNTSPECSLYSTTVVPGKTYRLRISSLTALSALSFQIEGHSL 270

Query: 244 TVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIF 303
           TVVEADGHYVEPF V NLFIYSGETYSVL+K DQDPSRNYW TTNVVSR+ TTPPGLAIF
Sbjct: 271 TVVEADGHYVEPFVVKNLFIYSGETYSVLVKTDQDPSRNYWATTNVVSRNATTPPGLAIF 330

Query: 304 NYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQNT 363
           NYYPNH  R PPT PP+ P WND+ PR  QS AIKAR+G I++PP  +D+VIV LNTQN 
Sbjct: 331 NYYPNHPRRSPPTIPPSGPMWNDIEPRFNQSVAIKARKGHIYSPPATSDRVIVFLNTQNR 390

Query: 364 VNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATV 423
           VNG  RWS+NNVSFN+PHTPYLIALK NLLHTFSQ  PP GYDFKNYDIF    N  AT 
Sbjct: 391 VNGNVRWSVNNVSFNIPHTPYLIALKENLLHTFSQTPPPEGYDFKNYDIFARQNNTNATT 450

Query: 424 SDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYN 483
           SDAIYRL  NSTVDIILQNAN M+PNNSETHPWHLHGHDFWVLGYG+GKFD   DP  YN
Sbjct: 451 SDAIYRLQLNSTVDIILQNANTMNPNNSETHPWHLHGHDFWVLGYGKGKFDPINDPKNYN 510

Query: 484 LENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPT 543
           L +PIMKNTVP+HP GWTALRF ADNPG WAFHCHI++HFFMGMGVVFEEGIERVGKLP+
Sbjct: 511 LVDPIMKNTVPVHPLGWTALRFKADNPGAWAFHCHIESHFFMGMGVVFEEGIERVGKLPS 570

Query: 544 SIMGCGESKRFLRP 556
           SIMGCGE+K  L+P
Sbjct: 571 SIMGCGETKHLLKP 582

BLAST of Cucsa.104600 vs. TrEMBL
Match: Q9ZR60_CUCME (L-ascorbate oxidase (Fragment) OS=Cucumis melo GN=ao1 PE=3 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 6.5e-255
Identity = 417/432 (96.53%), Postives = 425/432 (98.38%), Query Frame = 1

Query: 124 GSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQG 183
           GSIVVWVPEGQTEPFSYD DRNLILTDWYHKSS+EHA GLATPGSGF WVGEPDSLLIQG
Sbjct: 1   GSIVVWVPEGQTEPFSYDHDRNLILTDWYHKSSNEHAAGLATPGSGFTWVGEPDSLLIQG 60

Query: 184 RGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTV 243
           RGRFNCS+LGSSSGTCNSSSTECVL+PLTVVPGKTYRLRVSSLTSLSALSFQ+EGH+MTV
Sbjct: 61  RGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTYRLRVSSLTSLSALSFQVEGHSMTV 120

Query: 244 VEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNY 303
           VEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNY
Sbjct: 121 VEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNY 180

Query: 304 YPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQNTVN 363
           YPNHQS+ PPTTPPAPPAWNDV PRLAQSQAIKARQGFIH PPKAADKVIVLLNTQNTVN
Sbjct: 181 YPNHQSKRPPTTPPAPPAWNDVPPRLAQSQAIKARQGFIHAPPKAADKVIVLLNTQNTVN 240

Query: 364 GRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSD 423
           GRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSD
Sbjct: 241 GRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSD 300

Query: 424 AIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYNLE 483
           AI+RLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFD+Y DPSKYNLE
Sbjct: 301 AIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYNDPSKYNLE 360

Query: 484 NPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSI 543
           NPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSI
Sbjct: 361 NPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSI 420

Query: 544 MGCGESKRFLRP 556
           MGCGESKRFLRP
Sbjct: 421 MGCGESKRFLRP 432

BLAST of Cucsa.104600 vs. TrEMBL
Match: A0A061DIV4_THECC (Cupredoxin superfamily protein isoform 1 OS=Theobroma cacao GN=TCM_001179 PE=3 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 1.8e-252
Identity = 407/552 (73.73%), Postives = 460/552 (83.33%), Query Frame = 1

Query: 7   KSRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTENVAI 66
           ++R   YKWEVK E +SPDC KK+ +TING++PGPTI A Q DT+IVEL N+L TENVAI
Sbjct: 30  EARIRRYKWEVKYEYRSPDCLKKLVITINGQTPGPTIRAQQNDTIIVELTNNLSTENVAI 89

Query: 67  HWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSI 126
           HWHGIRQIGTPW DGTEGVTQCPILPG TF+YQFVVDRPGTYLYHAHYGMQRE GLYGSI
Sbjct: 90  HWHGIRQIGTPWFDGTEGVTQCPILPGTTFKYQFVVDRPGTYLYHAHYGMQREAGLYGSI 149

Query: 127 VVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQGRGR 186
           +V +P+GQ+EPF+YD DRN+IL DWYHKS++E A GL+     F WVGEP SLLI GRGR
Sbjct: 150 IVALPDGQSEPFTYDYDRNIILNDWYHKSTYEQAAGLSA--IPFQWVGEPQSLLIHGRGR 209

Query: 187 FNCSILG---SSSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTV 246
           FNCS L    S +G CN+++ +C    L VVPGKTYRLR+SSLT+LSALSFQIEGH MTV
Sbjct: 210 FNCSSLTIPTSDTGVCNTTNPDCAPFRLIVVPGKTYRLRISSLTALSALSFQIEGHNMTV 269

Query: 247 VEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNY 306
           VEADGHYVEPF V NLFIYSGETYSVL+KADQDP+RNYWIT+N+VSR   T PGLAIFNY
Sbjct: 270 VEADGHYVEPFVVQNLFIYSGETYSVLVKADQDPTRNYWITSNIVSRPANTTPGLAIFNY 329

Query: 307 YPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQNTVN 366
           YPNH  R PPT+PPA P WN+  PR+AQSQAIKAR G+IH PP  +D+VIV LNTQN +N
Sbjct: 330 YPNHPRRSPPTSPPAAPVWNNSRPRIAQSQAIKARPGYIHPPPLLSDRVIVFLNTQNRIN 389

Query: 367 GRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSD 426
           G  RWS+NNVSF +PHTPYLIALK NL H F Q  PP GYDF NYDIF    N  AT+ +
Sbjct: 390 GNVRWSVNNVSFTLPHTPYLIALKENLTHAFDQNPPPDGYDFVNYDIFKEQENKNATLGN 449

Query: 427 AIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYNLE 486
            IYRL+FNSTVDIILQNAN M+ NNSETHPWHLHGHDFWVLGYGEGKFD++ DP KYNL 
Sbjct: 450 GIYRLNFNSTVDIILQNANTMTKNNSETHPWHLHGHDFWVLGYGEGKFDMFNDPRKYNLV 509

Query: 487 NPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSI 546
           NPIMKNTVP+HPYGWTALRF ADNPG WAFHCHI++HF+MGMGVVF EGIE+VG LP+SI
Sbjct: 510 NPIMKNTVPVHPYGWTALRFKADNPGAWAFHCHIESHFYMGMGVVFAEGIEKVGNLPSSI 569

Query: 547 MGCGESKRFLRP 556
           MGCGE++   RP
Sbjct: 570 MGCGETQGLYRP 579

BLAST of Cucsa.104600 vs. TAIR10
Match: AT4G39830.1 (AT4G39830.1 Cupredoxin superfamily protein)

HSP 1 Score: 798.1 bits (2060), Expect = 3.4e-231
Identity = 380/558 (68.10%), Postives = 441/558 (79.03%), Query Frame = 1

Query: 2   ASPLQKSRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFT 61
           +S L + +   +KWEVK E KSPDCF+K+ +TING+ PGPTI A QGDT++VELKNS  T
Sbjct: 27  SSVLCQGKIRRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQGDTIVVELKNSFMT 86

Query: 62  ENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDG 121
           ENVA+HWHGIRQIGTPW DG EGVTQCPILPGE F YQFVVDRPGTY+YH+HYGMQRE G
Sbjct: 87  ENVAVHWHGIRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGTYMYHSHYGMQRESG 146

Query: 122 LYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLI 181
           L G I V  P  + EPF+YD DRN +LTDWYHKS  E ATGLA+    F WVGEP SL+I
Sbjct: 147 LIGMIQVSPPATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLAS--IPFKWVGEPQSLMI 206

Query: 182 QGRGRFNCSILGSS-----SGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQI 241
           QGRGRFNCS   ++     SG CN S+ +C    LTV+PGKTYRLR+ SLT+LSALSFQI
Sbjct: 207 QGRGRFNCSNNLTTPPSLVSGVCNVSNADCSRFILTVIPGKTYRLRIGSLTALSALSFQI 266

Query: 242 EGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPP 301
           EGH +TVVEADGHYVEPF V NLF+YSGETYSVL+KADQ+P RNYWIT+++VSR  TTPP
Sbjct: 267 EGHNLTVVEADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWITSSIVSRPATTPP 326

Query: 302 GLAIFNYYPNHQSRLPPTTPPA--PPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIV 361
             A+ NYYPNH  R PPT+  +   P WND   RLAQS AIKAR+GFIH  P+ +DKVIV
Sbjct: 327 ATAVLNYYPNHPRRRPPTSESSNIVPEWNDTRSRLAQSLAIKARRGFIHALPENSDKVIV 386

Query: 362 LLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFS-QEKPPTGYDFKNYDIFNP 421
           LLNTQN VNG RRWS+NNVS++ P TPYLIALK NL + F  +   P  YD +NYDIF  
Sbjct: 387 LLNTQNEVNGYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTAPENYDSRNYDIFAK 446

Query: 422 APNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDL 481
             N  AT SD IYRL FNSTVD+ILQNAN M+ NNSETHPWHLHGHDFWVLGYGEGKF+ 
Sbjct: 447 PLNANATTSDGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGHDFWVLGYGEGKFNE 506

Query: 482 YKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGI 541
            +DP +YN  +PI KNTV + P+GWTALRF ADNPGVW+FHCHI++HFFMGMG+VFE GI
Sbjct: 507 SEDPKRYNRVDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCHIESHFFMGMGIVFESGI 566

Query: 542 ERVGKLPTSIMGCGESKR 552
           ++V  LP+SIMGCG++KR
Sbjct: 567 DKVSSLPSSIMGCGQTKR 582

BLAST of Cucsa.104600 vs. TAIR10
Match: AT5G21105.1 (AT5G21105.1 Plant L-ascorbate oxidase)

HSP 1 Score: 581.6 bits (1498), Expect = 5.0e-166
Identity = 275/547 (50.27%), Postives = 364/547 (66.54%), Query Frame = 1

Query: 13  YKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTENVAIHWHGIR 72
           Y WEV+ +  SPDC +   +T+NG  PGPTI A  GDT++V L N L TE + IHWHGIR
Sbjct: 40  YHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTEGLVIHWHGIR 99

Query: 73  QIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPE 132
           Q G+PW+DG  GVTQC I PGETF Y F V++PGT+ YH HYGMQR  GLYGS++V V +
Sbjct: 100 QFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAGLYGSLIVDVAK 159

Query: 133 GQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQGRGRFNCSIL 192
           G++E   YD + NL+L+DW+H++      GL++      W+GE  S+LI GRG+FNCS+ 
Sbjct: 160 GKSERLRYDGEFNLLLSDWWHEAIPSQELGLSS--KPMRWIGEAQSILINGRGQFNCSLA 219

Query: 193 GSSSGTCN------SSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEA 252
              S   +          +C    L V P KTYR+R+SS T+L++L+  ++GH + VVEA
Sbjct: 220 AQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAVQGHKLVVVEA 279

Query: 253 DGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNYYPN 312
           DG+Y+ PF  +++ IYSGE+YSVL+  DQDPS+NY+I+  V  R   T   L I NY   
Sbjct: 280 DGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQALTILNYVTA 339

Query: 313 HQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQNTVNGRR 372
             S+LP + PP  P W+D       S+ I +  G   +PPK   K ++LLNTQN ++G  
Sbjct: 340 PASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGS-PSPPKKYRKRLILLNTQNLIDGYT 399

Query: 373 RWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIY 432
           +W++NNVS   P TPYL ++K+NL   F+++ PP  Y   +YDI NP P P  T  + IY
Sbjct: 400 KWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM-DYDIMNPPPFPNTTTGNGIY 459

Query: 433 RLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYNLENPI 492
              FN TVD+I+QNAN++    SE HPWHLHGHDFWVLGYG+GKF    D   YNL+NP 
Sbjct: 460 VFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEKTYNLKNPP 519

Query: 493 MKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSIMGC 552
           ++NT  L+PYGWTA+RFV DNPGVW FHCHI+ H  MGMGVVF EG+ R+GK+P   +GC
Sbjct: 520 LRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGKVPDEALGC 579

Query: 553 GESKRFL 554
           G +K+FL
Sbjct: 580 GLTKQFL 582

BLAST of Cucsa.104600 vs. TAIR10
Match: AT5G21100.1 (AT5G21100.1 Plant L-ascorbate oxidase)

HSP 1 Score: 561.6 bits (1446), Expect = 5.4e-160
Identity = 272/547 (49.73%), Postives = 356/547 (65.08%), Query Frame = 1

Query: 15  WEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQI 74
           WEV+ +   PDC + + + ING+ PGPTI A  GDTVI+ + N L TE V IHWHGIRQ 
Sbjct: 28  WEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVAGDTVIIHVVNKLSTEGVVIHWHGIRQK 87

Query: 75  GTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQ 134
           GTPW+DG  GVTQCPI PGETF Y+F+VD+ GT+ YH HYGMQR  GLYG ++V  P+  
Sbjct: 88  GTPWADGAAGVTQCPINPGETFTYKFIVDKAGTHFYHGHYGMQRSSGLYGMLIVRSPK-- 147

Query: 135 TEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQGRGRFNCSIL-- 194
            E   YD + NL+L+DW+H+S H  A  LA       W+GEP SLLI GRG+FNCS    
Sbjct: 148 -ERLIYDGEFNLLLSDWWHQSIH--AQELALSSRPMRWIGEPQSLLINGRGQFNCSQAAY 207

Query: 195 ---GSSSGTCN-SSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADG 254
              G     C    + +C    L V P + YRLR++S T+L++L+  ++GH + VVEADG
Sbjct: 208 FNKGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRIASTTALASLNLAVQGHQLVVVEADG 267

Query: 255 HYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQ 314
           +YV PF VN++ +YSGETYSVL+K +  PS+ YWI+  V  R   TP  L + NY    +
Sbjct: 268 NYVAPFTVNDIDVYSGETYSVLLKTNALPSKKYWISVGVRGREPKTPQALTVINYVDATE 327

Query: 315 SRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQNTVNGRRRW 374
           SR P   PP  P WND     + S+ I A +G+   P K+ D+ ++LLNTQN      +W
Sbjct: 328 SR-PSHPPPVTPIWNDTDRSKSFSKKIFAAKGYPKPPEKSHDQ-LILLNTQNLYEDYTKW 387

Query: 375 SLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIYRL 434
           S+NNVS ++P TPYL ++++ L   +  + P       NYDI  P PNP  T    IY  
Sbjct: 388 SINNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKKLIMDNYDIMKPPPNPNTTKGSGIYNF 447

Query: 435 DFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYNLENPIMK 494
            F   VD+ILQNAN++    SE HPWH+HGHDFWVLGYGEGKF    D   +NL+NP ++
Sbjct: 448 AFGIVVDVILQNANVLKGVISEIHPWHIHGHDFWVLGYGEGKFKPGIDEKTFNLKNPPLR 507

Query: 495 NTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGK--LPTSIMGC 554
           NTV L+P+GWTA+RFV DNPGVW FHCHI+ H  MGMGVVF EG++R+GK  +P   +GC
Sbjct: 508 NTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFVEGVDRIGKMEIPDEALGC 567

BLAST of Cucsa.104600 vs. TAIR10
Match: AT5G09360.1 (AT5G09360.1 laccase 14)

HSP 1 Score: 268.5 bits (685), Expect = 9.4e-72
Identity = 184/554 (33.21%), Postives = 289/554 (52.17%), Query Frame = 1

Query: 1   MASPLQKSRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLF 60
           +A  + ++   ++ +++KS+  +  C     LT+NG  PGPT+ A++GD +IV + N+  
Sbjct: 26  LAFQIAEAEIHHHTFKIKSKAYTRLCNTNKILTVNGEFPGPTLKAYRGDKLIVNVINNA- 85

Query: 61  TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVD-RPGTYLYHAHYGMQRE 120
             N+ +HWHG RQI  PWSDG E VTQCPI PGE++ Y+  +    GT  +HAH    R 
Sbjct: 86  NYNITLHWHGARQIRNPWSDGPEYVTQCPIRPGESYVYRIDLKVEEGTIWWHAHSQWARA 145

Query: 121 DGLYGSIVVWVPEGQTEPFSYD-LDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEP-- 180
             ++G+ +V+   G + PF     +  LIL +W+ K +  H      PG      GEP  
Sbjct: 146 T-VHGAFIVYPKRGSSYPFPKPHREIPLILGEWWKKENIMHI-----PGKANKTGGEPAI 205

Query: 181 -DSLLIQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQ 240
            DS  I G+  +           C+   T      +TVV G+ Y LR+ +      L F 
Sbjct: 206 SDSYTINGQPGYLYP--------CSKPET----FKITVVRGRRYLLRIINAVMDEELFFA 265

Query: 241 IEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRS---- 300
           I  HT+TVV  DG Y++ F+ + L I  G++  VL+ A+Q P+ +Y++     S +    
Sbjct: 266 IANHTLTVVAKDGFYLKHFKSDYLMITPGQSMDVLLHANQRPN-HYFVAARAYSSAFGAG 325

Query: 301 --RTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAA 360
             +TT    AI  Y  +  +R+ P  P  PP +N        +   ++++  ++ P K  
Sbjct: 326 FDKTTTT--AILQYKGDTLNRIKPILPYLPP-YNRTEASTRFTNQFRSQRP-VNVPVKIN 385

Query: 361 DKVI--VLLNTQNTVNGR-------RRWS--LNNVSFNMPHTPYLIALKHNLLHTFSQE- 420
            +++  + +N  N  + R       +R+S  +NN+SF  P    L A   ++   F ++ 
Sbjct: 386 TRLLYAISVNLMNCSDDRPCTGPFGKRFSSSINNISFVNPSVDILRAYYRHIGGVFQEDF 445

Query: 421 --KPPTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWH 480
              PPT +++   ++  P P    T    +  LD+NS+V++ILQ   + + N    HP H
Sbjct: 446 PRNPPTKFNYTGENL--PFPTRFGT---KVVVLDYNSSVELILQGTTVWASN---IHPIH 505

Query: 481 LHGHDFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHC 530
           LHG++F+V+G G G FD  KDP +YNL +P  + TV +   GWTA+RFVA+NPGVW  HC
Sbjct: 506 LHGYNFYVVGSGFGNFDRRKDPLRYNLVDPPEETTVGVPRNGWTAVRFVANNPGVWLLHC 547

BLAST of Cucsa.104600 vs. TAIR10
Match: AT5G05390.1 (AT5G05390.1 laccase 12)

HSP 1 Score: 265.8 bits (678), Expect = 6.1e-71
Identity = 175/529 (33.08%), Postives = 265/529 (50.09%), Query Frame = 1

Query: 26  CFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGV 85
           C  +  +T+NG  PGPT+  + GDT+ V++ N     N+ IHWHG+RQI T W+DG E V
Sbjct: 42  CKTRNAITVNGMFPGPTLEVNNGDTLEVKVHNRA-RYNITIHWHGVRQIRTGWADGPEFV 101

Query: 86  TQCPILPGETFRYQFVVD-RPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSY-DLD 145
           TQCPI PG+++ Y+F +  + GT  +HAH    R   +YG++++    G + PF   D  
Sbjct: 102 TQCPIRPGKSYTYRFTIQGQEGTLWWHAHSSWLRAT-VYGALIIHPTPGSSFPFPKPDRQ 161

Query: 146 RNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSSSGTCNSSS 205
             L+L +W++ +  +        G+  N + +  ++  Q    +NCS            +
Sbjct: 162 TALMLGEWWNANPVDVINQATRTGAAPN-ISDAYTINGQPGDLYNCS------------T 221

Query: 206 TECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYS 265
            E V+ P+    G+T  LRV +      L F +  H +TVV AD  Y++PF    L +  
Sbjct: 222 KETVVVPIN--SGETSLLRVINAALNQPLFFTVANHKLTVVGADASYLKPFTTKVLMLGP 281

Query: 266 GETYSVLIKADQDPSRNYWITTNVVSRSRTTP----PGLAIFNYYPNHQSRLPPTTPPAP 325
           G+T  VL+ ADQ P R Y+I       ++  P       AI  Y     +  P    P  
Sbjct: 282 GQTTDVLLTADQPPKR-YYIAARAYQSAQNAPFDNTTTTAILQYKKTTTTSKP--IMPVL 341

Query: 326 PAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVL---LNTQNT-----------VNGR 385
           PA+ND     + S+  K+ +  +   PK  D  +     L   N            +NG 
Sbjct: 342 PAFNDTNTVTSFSRKFKSLRNVV--VPKTIDDNLFFTIGLGLDNCPKKFPKSRCQGLNGT 401

Query: 386 R-RWSLNNVSFNMPHTPYLIALKHN----LLHTFSQEKPPTGYDFKNYDIFNPAPNPEAT 445
           R   S+NNVSF +P    L+    N    +  T    KPP  +D+   +I      P   
Sbjct: 402 RFTASMNNVSFVLPSNFSLLQAHSNGIPGVFTTDFPSKPPVKFDYTGNNISRALFQP--V 461

Query: 446 VSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKY 505
               +Y+L + S V ++LQ+ N+++   SE HP HLHG+DF+++G G G F+  KD SK+
Sbjct: 462 KGTKLYKLKYGSRVQVVLQDTNIVT---SENHPIHLHGYDFYIVGEGFGNFNPKKDTSKF 521

Query: 506 NLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVF 530
           NL +P ++NTV +   GW  +RFVADNPGVW  HCH+D H   G+ + F
Sbjct: 522 NLVDPPLRNTVAVPVNGWAVIRFVADNPGVWLMHCHLDVHIKWGLAMAF 543

BLAST of Cucsa.104600 vs. NCBI nr
Match: gi|449451465|ref|XP_004143482.1| (PREDICTED: L-ascorbate oxidase [Cucumis sativus])

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 549/555 (98.92%), Postives = 551/555 (99.28%), Query Frame = 1

Query: 1   MASPLQKSRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLF 60
           + S   +SRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLF
Sbjct: 22  LQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLF 81

Query: 61  TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQRED 120
           TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQRED
Sbjct: 82  TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQRED 141

Query: 121 GLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLL 180
           GLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLL
Sbjct: 142 GLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLL 201

Query: 181 IQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHT 240
           IQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHT
Sbjct: 202 IQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHT 261

Query: 241 MTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAI 300
           MTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAI
Sbjct: 262 MTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAI 321

Query: 301 FNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQN 360
           FNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQN
Sbjct: 322 FNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQN 381

Query: 361 TVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEAT 420
           TVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEAT
Sbjct: 382 TVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEAT 441

Query: 421 VSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKY 480
           VSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKY
Sbjct: 442 VSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKY 501

Query: 481 NLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLP 540
           NLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLP
Sbjct: 502 NLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLP 561

Query: 541 TSIMGCGESKRFLRP 556
           TSIMGCGESKRFLRP
Sbjct: 562 TSIMGCGESKRFLRP 576

BLAST of Cucsa.104600 vs. NCBI nr
Match: gi|659080148|ref|XP_008440636.1| (PREDICTED: L-ascorbate oxidase [Cucumis melo])

HSP 1 Score: 1130.9 bits (2924), Expect = 0.0e+00
Identity = 531/555 (95.68%), Postives = 543/555 (97.84%), Query Frame = 1

Query: 1   MASPLQKSRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLF 60
           + S   +SRTLNYKWEVKSELKSPDCFKK+T+TINGRSPGPTIYAHQGDTVIVELKNSL 
Sbjct: 22  LQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSPGPTIYAHQGDTVIVELKNSLH 81

Query: 61  TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQRED 120
           TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQRED
Sbjct: 82  TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQRED 141

Query: 121 GLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLL 180
           GLYGSIVVWVPEGQTEPFSYD DRNLILTDWYHKSS+EHA GLATPGSGF WVGEPDSLL
Sbjct: 142 GLYGSIVVWVPEGQTEPFSYDHDRNLILTDWYHKSSNEHAAGLATPGSGFTWVGEPDSLL 201

Query: 181 IQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHT 240
           IQGRGRFNCS+LGSSSGTCNSSSTECVL+PLTVVPGKTYRLRVSSLTSLSALSFQIEGH+
Sbjct: 202 IQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHS 261

Query: 241 MTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAI 300
           MTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAI
Sbjct: 262 MTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAI 321

Query: 301 FNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQN 360
           FNYYPNHQS+ PPTTPPAPPAWNDV PRLAQSQAIKARQGFIH PPKAADKVIVLLNTQN
Sbjct: 322 FNYYPNHQSKRPPTTPPAPPAWNDVPPRLAQSQAIKARQGFIHAPPKAADKVIVLLNTQN 381

Query: 361 TVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEAT 420
           TVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEAT
Sbjct: 382 TVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEAT 441

Query: 421 VSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKY 480
           VSDAI+RLDFNSTVD+ILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFD+Y DPSKY
Sbjct: 442 VSDAIFRLDFNSTVDLILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYNDPSKY 501

Query: 481 NLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLP 540
           NLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLP
Sbjct: 502 NLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLP 561

Query: 541 TSIMGCGESKRFLRP 556
           TSIMGCGESKRFLRP
Sbjct: 562 TSIMGCGESKRFLRP 576

BLAST of Cucsa.104600 vs. NCBI nr
Match: gi|566179811|ref|XP_006380435.1| (L-ascorbate oxidase family protein [Populus trichocarpa])

HSP 1 Score: 895.2 bits (2312), Expect = 5.8e-257
Identity = 417/554 (75.27%), Postives = 470/554 (84.84%), Query Frame = 1

Query: 4   PLQKSRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTEN 63
           P+  +R  +YKWE+K E KSPDC+KK+ +TINGR+PGPTI A Q DTVIVE+KNSL TEN
Sbjct: 17  PIADARIRHYKWELKYEYKSPDCYKKLVITINGRTPGPTILAKQNDTVIVEVKNSLLTEN 76

Query: 64  VAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLY 123
            AIHWHGIRQIGTPW DGTEGVTQCPILPG+TF Y+FVVDRPGTYLYHAHYGMQRE G+Y
Sbjct: 77  TAIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFVYKFVVDRPGTYLYHAHYGMQREAGIY 136

Query: 124 GSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQG 183
           GSI V +P+G++EPF+YD DR++ILTDWYHKS++E A GL++    F WVGEP SLLIQG
Sbjct: 137 GSIRVALPDGESEPFAYDYDRSIILTDWYHKSTYEQAAGLSS--IPFQWVGEPQSLLIQG 196

Query: 184 RGRFNCSILGS--SSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTM 243
           +GRF+CS       +  CN+++ EC L+  TVVPGKTYRLR+SSL++LSALSFQIEGH M
Sbjct: 197 KGRFDCSAANPPLKADVCNNTNPECSLYSTTVVPGKTYRLRISSLSALSALSFQIEGHNM 256

Query: 244 TVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIF 303
           TVVEADGHYVEPF V NLFIYSGETYSVL+K DQDPSRNYW TTNVVSR+ TTPPGLAIF
Sbjct: 257 TVVEADGHYVEPFVVKNLFIYSGETYSVLVKTDQDPSRNYWATTNVVSRNATTPPGLAIF 316

Query: 304 NYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQNT 363
           NYYPNH  R PPT PP+ P WND+ PR  QS AIKAR+G I+ PP  +D+VIVLLNTQNT
Sbjct: 317 NYYPNHPRRSPPTIPPSGPMWNDIAPRFNQSVAIKARRGHIYPPPATSDRVIVLLNTQNT 376

Query: 364 VNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATV 423
           VNG  RWS+N VSFN+PHTPYLIALK NLLHTFSQ  PP GYDFKNYDIF    N  AT 
Sbjct: 377 VNGNVRWSVNKVSFNIPHTPYLIALKENLLHTFSQTPPPEGYDFKNYDIFARQNNTNATT 436

Query: 424 SDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYN 483
           SDAIYRL  NSTVDIILQNAN M+PNNSETHPWHLHGHDFWVLGYG+GKFD   DP  YN
Sbjct: 437 SDAIYRLQLNSTVDIILQNANTMNPNNSETHPWHLHGHDFWVLGYGKGKFDPINDPKNYN 496

Query: 484 LENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPT 543
           L +PIMKNTVP+HP+GWTALRF ADNPGVWAFHCHI++HFFMGMGVVFEEGIERVGKLP+
Sbjct: 497 LVDPIMKNTVPVHPFGWTALRFKADNPGVWAFHCHIESHFFMGMGVVFEEGIERVGKLPS 556

Query: 544 SIMGCGESKRFLRP 556
           SIMGCGE+KR L+P
Sbjct: 557 SIMGCGETKRLLKP 568

BLAST of Cucsa.104600 vs. NCBI nr
Match: gi|743923090|ref|XP_011005626.1| (PREDICTED: L-ascorbate oxidase-like [Populus euphratica])

HSP 1 Score: 893.3 bits (2307), Expect = 2.2e-256
Identity = 414/554 (74.73%), Postives = 471/554 (85.02%), Query Frame = 1

Query: 4   PLQKSRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTEN 63
           P+ ++R  +YKWE+K E KSPDC+KK+ +TINGR+PGPTI A Q DTVIVE+KNSL TEN
Sbjct: 31  PIAEARIRHYKWELKYEYKSPDCYKKLVITINGRTPGPTILAKQNDTVIVEVKNSLLTEN 90

Query: 64  VAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLY 123
            AIHWHGIRQIGTPW DGTEGVTQCPILPG+TF Y+FVVDRPGTYLYHAHYGMQRE G+Y
Sbjct: 91  TAIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFVYKFVVDRPGTYLYHAHYGMQREAGIY 150

Query: 124 GSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQG 183
           GSI V +P+G++EPF+YD DR++ILTDWYHKS++EHA GL++    F WVGEP SLLIQG
Sbjct: 151 GSIRVALPDGESEPFAYDYDRSIILTDWYHKSTYEHAAGLSS--IPFQWVGEPQSLLIQG 210

Query: 184 RGRFNCSILGS--SSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTM 243
           +GRF+C+       +  CN+++ EC L+  TVVPGKTYRLR+SSL++LSALSFQIEGH+M
Sbjct: 211 KGRFDCAAANPPLKADVCNNTNPECSLYSTTVVPGKTYRLRISSLSALSALSFQIEGHSM 270

Query: 244 TVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIF 303
           TVVEADGHYVEPF V NLFIYSGETYSVL+K DQDPSRNYW  TNVVSR+ TTPPGLAIF
Sbjct: 271 TVVEADGHYVEPFVVKNLFIYSGETYSVLVKTDQDPSRNYWAATNVVSRNATTPPGLAIF 330

Query: 304 NYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQNT 363
           NYYPNH  R PPT PP+ P WND+ PR  QS AIKAR+G I+ PP  +D+VIVLLNTQN 
Sbjct: 331 NYYPNHPRRSPPTIPPSGPMWNDIEPRFNQSVAIKARKGHIYYPPATSDRVIVLLNTQNR 390

Query: 364 VNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATV 423
           VNG  RWS+NNVSFN+PHTPYLIALK NLLHTFSQ  PP GYDFKNYD+F    N  AT 
Sbjct: 391 VNGNVRWSVNNVSFNIPHTPYLIALKENLLHTFSQTPPPEGYDFKNYDVFARQNNTNATT 450

Query: 424 SDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYN 483
           SDAIYRL  NSTVDIILQNAN M+PNNSETHPWHLHGHDFWVLGYG+GKFD   DP  YN
Sbjct: 451 SDAIYRLQLNSTVDIILQNANTMNPNNSETHPWHLHGHDFWVLGYGKGKFDPINDPKNYN 510

Query: 484 LENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPT 543
           L +PIMKNTVP+HP+GWTALRF ADNPGVWAFHCHI++HFFMGMGVVFEEGIERVGKLP+
Sbjct: 511 LVDPIMKNTVPVHPFGWTALRFKADNPGVWAFHCHIESHFFMGMGVVFEEGIERVGKLPS 570

Query: 544 SIMGCGESKRFLRP 556
           SIMGCGE+K  L+P
Sbjct: 571 SIMGCGETKHLLKP 582

BLAST of Cucsa.104600 vs. NCBI nr
Match: gi|566179815|ref|XP_002309992.2| (hypothetical protein POPTR_0007s05910g [Populus trichocarpa])

HSP 1 Score: 891.7 bits (2303), Expect = 6.4e-256
Identity = 415/554 (74.91%), Postives = 471/554 (85.02%), Query Frame = 1

Query: 4   PLQKSRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTEN 63
           P+ ++R  +YK+E+K E KSPDC+KK+ +TINGR+PGPTI+A Q DTVIVE+KNSL TEN
Sbjct: 31  PIAEARIRHYKFELKYEYKSPDCYKKLVITINGRTPGPTIFAQQNDTVIVEVKNSLLTEN 90

Query: 64  VAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLY 123
            AIHWHGIRQIGTPW DGTEGVTQCPILPG+TF Y+FVVDRPGTYLYHAHYGMQRE G+Y
Sbjct: 91  TAIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFVYKFVVDRPGTYLYHAHYGMQREAGIY 150

Query: 124 GSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQG 183
           GSI V +P+G++EPF+YD DR++ILTDWYHKS++E ATGL++    F WVGEP SLLIQG
Sbjct: 151 GSIRVALPDGESEPFAYDYDRSIILTDWYHKSTYEQATGLSS--IPFQWVGEPQSLLIQG 210

Query: 184 RGRFNCSILGS--SSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTM 243
           +GRF+CS       +  CN++S EC L+  TVVPGKTYRLR+SSLT+LSALSFQIEGH++
Sbjct: 211 KGRFDCSAANPPLKADVCNNTSPECSLYSTTVVPGKTYRLRISSLTALSALSFQIEGHSL 270

Query: 244 TVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIF 303
           TVVEADGHYVEPF V NLFIYSGETYSVL+K DQDPSRNYW TTNVVSR+ TTPPGLAIF
Sbjct: 271 TVVEADGHYVEPFVVKNLFIYSGETYSVLVKTDQDPSRNYWATTNVVSRNATTPPGLAIF 330

Query: 304 NYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQNT 363
           NYYPNH  R PPT PP+ P WND+ PR  QS AIKAR+G I++PP  +D+VIV LNTQN 
Sbjct: 331 NYYPNHPRRSPPTIPPSGPMWNDIEPRFNQSVAIKARKGHIYSPPATSDRVIVFLNTQNR 390

Query: 364 VNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATV 423
           VNG  RWS+NNVSFN+PHTPYLIALK NLLHTFSQ  PP GYDFKNYDIF    N  AT 
Sbjct: 391 VNGNVRWSVNNVSFNIPHTPYLIALKENLLHTFSQTPPPEGYDFKNYDIFARQNNTNATT 450

Query: 424 SDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYN 483
           SDAIYRL  NSTVDIILQNAN M+PNNSETHPWHLHGHDFWVLGYG+GKFD   DP  YN
Sbjct: 451 SDAIYRLQLNSTVDIILQNANTMNPNNSETHPWHLHGHDFWVLGYGKGKFDPINDPKNYN 510

Query: 484 LENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPT 543
           L +PIMKNTVP+HP GWTALRF ADNPG WAFHCHI++HFFMGMGVVFEEGIERVGKLP+
Sbjct: 511 LVDPIMKNTVPVHPLGWTALRFKADNPGAWAFHCHIESHFFMGMGVVFEEGIERVGKLPS 570

Query: 544 SIMGCGESKRFLRP 556
           SIMGCGE+K  L+P
Sbjct: 571 SIMGCGETKHLLKP 582