Csa6G500640 (gene) Cucumber (Chinese Long) v2

NameCsa6G500640
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionAscorbate oxidase; contains IPR017760 (L-ascorbate oxidase, plants)
LocationChr6 : 25022668 .. 25024928 (+)
   



The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAAAATGTAAAAAAGTTCAAATGAAAATGTCGCAGCCGCTGCCTTGGACCAAGATTTGATTGGGTCAAAGTCATGCAGGATATTTATATAGAACCAATGCTTTCCCCAAAGCATCAACCTCAATGATTTTTAAGCCTTTTGGGTTTTAGAAGAAGCACTCAAACGATTCTAACAATGGCTTCGCCGTTACAAAGTACACGATACCTCTTAATTCTTCTCTCCTTTGTCTTCTTCTTACTACAATCTCAAACTGCAGAATCAAGAACTCTGAACTATAAATGGGAAGTGAAATCTGAATTGAAGTCCCCCGATTGCTTTAAAAAAGTTACCTTGACCATTAATGGCCGATCTCCAGGTCCCACTATCTACGCCCACCAAGGTGACACCGTCATCGTTGAGCTCAAGAACAGCCTCTTTACCGAAAACGTGGCTATCCACTGGCATGGAATTCGACAGGTACCCCTAAATTTTCATTTCTCTCCATATTTTTCTTCGTTAGTTTTATAGTTCTTCTTTCTGACGTGCTCGTACATTTGAAGATCGGAACACCATGGAGCGATGGAACAGAAGGTGTCACTCAATGCCCTATTTTGCCTGGTGAAACCTTCAGATATCAGTTTGTTGTAGACAGAGTAAGAGATACTTGACTTACTCGTTTTGTTTTGTGTCTCTCAAAAACTCCATGCTTTAATTTGAGTTTGACCTTTGTGAAATTTGAAATCTGCAGCCAGGAACTTATCTATACCATGCCCATTATGGTATGCAGAGAGAAGATGGTCTCTATGGATCCATTGTGGTTTGGGTTCCAGAAGGACAAACAGAGCCTTTTTCCTATGATCTTGATCGTAACCTCATCCTCACAGATTGGTATCATAAAAGCAGCCACGAACACGCCACTGGATTGGCTACCCCTGGCTCTGGATTCAACTGGGTGGGAGAGCCTGATTCACTGTTGATTCAAGGAAGAGGGAGATTCAACTGCTCTATTCTGGGATCTTCCAGTGGTACCTGCAATTCATCGAGCACAGAATGTGTTCTTCATCCATTAACAGTCGTACCGGGAAAAACTTACCGATTAAGGGTCTCCAGTCTCACTTCTCTTTCAGCTCTCAGTTTCCAAATTGAGGTAATTACAAGTTCATCTACTTGATTTCATGTACTCTCCTTGAAATTCTTCTTAAATGGCTTAAACTGATAAACTCTCTGTTTTGGAAACAGGGTCACACCATGACTGTGGTTGAAGCTGATGGGCATTACGTTGAGCCATTTCAAGTAAACAATTTGTTCATTTACTCAGGAGAAACATATTCCGTTTTGATAAAAGCCGATCAAGACCCTTCTAGAAACTACTGGATCACAACCAATGTGGTGAGCCGATCAAGAACCACCCCCCCTGGATTAGCCATTTTCAATTACTACCCAAATCACCAGAGTAGACTCCCTCCGACAACTCCTCCAGCACCACCTGCTTGGAACGATGTTACTCCCCGATTGGCTCAAAGTCAAGCCATCAAAGCCCGGCAAGGTTTTATCCACACGCCGCCCAAAGCCGCCGATAAAGTGATCGTGCTTCTCAACACCCAAAACACCGTGAATGGCCGTCGCCGGTGGTCGTTAAATAACGTTTCCTTCAATATGCCTCATACCCCATATCTTATTGCTCTAAAGCACAATCTTCTCCATACATTCAGCCAGGAAAAACCACCCACTGGATACGATTTCAAGAACTACGACATTTTTAATCCGGCGCCCAACCCTGAAGCGACGGTAAGCGACGCCATATACCGGTTGGACTTCAATTCAACTGTGGACATCATACTACAGAACGCCAATATGATGAGCCCAAACAACAGCGAAACACATCCTTGGCATTTGCACGGACACGATTTCTGGGTTTTGGGGTACGGAGAAGGGAAATTCGATCTTTATAAGGATCCAAGCAAGTACAATTTGGAGAACCCGATCATGAAGAACACGGTGCCGTTGCATCCATATGGATGGACCGCTTTGAGATTTGTGGCGGATAATCCTGGTGTCTGGGCTTTCCACTGTCACATCGACGCGCATTTCTTTATGGGAATGGGTGTAGTTTTTGAAGAAGGGATCGAGAGGGTTGGAAAATTGCCTACTTCCATTATGGGATGCGGGGAGAGCAAGAGGTTCTTGAGACCTTAACTCATTTCCATTCCGATTACTATTCTTTAGAAAAACACAAAGAATATTCTTTTATGTTTAGCCAAATTTTTTGTGCTGATTGAGA

mRNA sequence

ATGGCTTCGCCGTTACAAAGTACACGATACCTCTTAATTCTTCTCTCCTTTGTCTTCTTCTTACTACAATCTCAAACTGCAGAATCAAGAACTCTGAACTATAAATGGGAAGTGAAATCTGAATTGAAGTCCCCCGATTGCTTTAAAAAAGTTACCTTGACCATTAATGGCCGATCTCCAGGTCCCACTATCTACGCCCACCAAGGTGACACCGTCATCGTTGAGCTCAAGAACAGCCTCTTTACCGAAAACGTGGCTATCCACTGGCATGGAATTCGACAGATCGGAACACCATGGAGCGATGGAACAGAAGGTGTCACTCAATGCCCTATTTTGCCTGGTGAAACCTTCAGATATCAGTTTGTTGTAGACAGACCAGGAACTTATCTATACCATGCCCATTATGGTATGCAGAGAGAAGATGGTCTCTATGGATCCATTGTGGTTTGGGTTCCAGAAGGACAAACAGAGCCTTTTTCCTATGATCTTGATCGTAACCTCATCCTCACAGATTGGTATCATAAAAGCAGCCACGAACACGCCACTGGATTGGCTACCCCTGGCTCTGGATTCAACTGGGTGGGAGAGCCTGATTCACTGTTGATTCAAGGAAGAGGGAGATTCAACTGCTCTATTCTGGGATCTTCCAGTGGTACCTGCAATTCATCGAGCACAGAATGTGTTCTTCATCCATTAACAGTCGTACCGGGAAAAACTTACCGATTAAGGGTCTCCAGTCTCACTTCTCTTTCAGCTCTCAGTTTCCAAATTGAGGGTCACACCATGACTGTGGTTGAAGCTGATGGGCATTACGTTGAGCCATTTCAAGTAAACAATTTGTTCATTTACTCAGGAGAAACATATTCCGTTTTGATAAAAGCCGATCAAGACCCTTCTAGAAACTACTGGATCACAACCAATGTGGTGAGCCGATCAAGAACCACCCCCCCTGGATTAGCCATTTTCAATTACTACCCAAATCACCAGAGTAGACTCCCTCCGACAACTCCTCCAGCACCACCTGCTTGGAACGATGTTACTCCCCGATTGGCTCAAAGTCAAGCCATCAAAGCCCGGCAAGGTTTTATCCACACGCCGCCCAAAGCCGCCGATAAAGTGATCGTGCTTCTCAACACCCAAAACACCGTGAATGGCCGTCGCCGGTGGTCGTTAAATAACGTTTCCTTCAATATGCCTCATACCCCATATCTTATTGCTCTAAAGCACAATCTTCTCCATACATTCAGCCAGGAAAAACCACCCACTGGATACGATTTCAAGAACTACGACATTTTTAATCCGGCGCCCAACCCTGAAGCGACGGTAAGCGACGCCATATACCGGTTGGACTTCAATTCAACTGTGGACATCATACTACAGAACGCCAATATGATGAGCCCAAACAACAGCGAAACACATCCTTGGCATTTGCACGGACACGATTTCTGGGTTTTGGGGTACGGAGAAGGGAAATTCGATCTTTATAAGGATCCAAGCAAGTACAATTTGGAGAACCCGATCATGAAGAACACGGTGCCGTTGCATCCATATGGATGGACCGCTTTGAGATTTGTGGCGGATAATCCTGGTGTCTGGGCTTTCCACTGTCACATCGACGCGCATTTCTTTATGGGAATGGGTGTAGTTTTTGAAGAAGGGATCGAGAGGGTTGGAAAATTGCCTACTTCCATTATGGGATGCGGGGAGAGCAAGAGGTTCTTGAGACCTTAA

Coding sequence (CDS)

ATGGCTTCGCCGTTACAAAGTACACGATACCTCTTAATTCTTCTCTCCTTTGTCTTCTTCTTACTACAATCTCAAACTGCAGAATCAAGAACTCTGAACTATAAATGGGAAGTGAAATCTGAATTGAAGTCCCCCGATTGCTTTAAAAAAGTTACCTTGACCATTAATGGCCGATCTCCAGGTCCCACTATCTACGCCCACCAAGGTGACACCGTCATCGTTGAGCTCAAGAACAGCCTCTTTACCGAAAACGTGGCTATCCACTGGCATGGAATTCGACAGATCGGAACACCATGGAGCGATGGAACAGAAGGTGTCACTCAATGCCCTATTTTGCCTGGTGAAACCTTCAGATATCAGTTTGTTGTAGACAGACCAGGAACTTATCTATACCATGCCCATTATGGTATGCAGAGAGAAGATGGTCTCTATGGATCCATTGTGGTTTGGGTTCCAGAAGGACAAACAGAGCCTTTTTCCTATGATCTTGATCGTAACCTCATCCTCACAGATTGGTATCATAAAAGCAGCCACGAACACGCCACTGGATTGGCTACCCCTGGCTCTGGATTCAACTGGGTGGGAGAGCCTGATTCACTGTTGATTCAAGGAAGAGGGAGATTCAACTGCTCTATTCTGGGATCTTCCAGTGGTACCTGCAATTCATCGAGCACAGAATGTGTTCTTCATCCATTAACAGTCGTACCGGGAAAAACTTACCGATTAAGGGTCTCCAGTCTCACTTCTCTTTCAGCTCTCAGTTTCCAAATTGAGGGTCACACCATGACTGTGGTTGAAGCTGATGGGCATTACGTTGAGCCATTTCAAGTAAACAATTTGTTCATTTACTCAGGAGAAACATATTCCGTTTTGATAAAAGCCGATCAAGACCCTTCTAGAAACTACTGGATCACAACCAATGTGGTGAGCCGATCAAGAACCACCCCCCCTGGATTAGCCATTTTCAATTACTACCCAAATCACCAGAGTAGACTCCCTCCGACAACTCCTCCAGCACCACCTGCTTGGAACGATGTTACTCCCCGATTGGCTCAAAGTCAAGCCATCAAAGCCCGGCAAGGTTTTATCCACACGCCGCCCAAAGCCGCCGATAAAGTGATCGTGCTTCTCAACACCCAAAACACCGTGAATGGCCGTCGCCGGTGGTCGTTAAATAACGTTTCCTTCAATATGCCTCATACCCCATATCTTATTGCTCTAAAGCACAATCTTCTCCATACATTCAGCCAGGAAAAACCACCCACTGGATACGATTTCAAGAACTACGACATTTTTAATCCGGCGCCCAACCCTGAAGCGACGGTAAGCGACGCCATATACCGGTTGGACTTCAATTCAACTGTGGACATCATACTACAGAACGCCAATATGATGAGCCCAAACAACAGCGAAACACATCCTTGGCATTTGCACGGACACGATTTCTGGGTTTTGGGGTACGGAGAAGGGAAATTCGATCTTTATAAGGATCCAAGCAAGTACAATTTGGAGAACCCGATCATGAAGAACACGGTGCCGTTGCATCCATATGGATGGACCGCTTTGAGATTTGTGGCGGATAATCCTGGTGTCTGGGCTTTCCACTGTCACATCGACGCGCATTTCTTTATGGGAATGGGTGTAGTTTTTGAAGAAGGGATCGAGAGGGTTGGAAAATTGCCTACTTCCATTATGGGATGCGGGGAGAGCAAGAGGTTCTTGAGACCTTAA

Protein sequence

MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP*
BLAST of Csa6G500640 vs. Swiss-Prot
Match: ASO_CUCSA (L-ascorbate oxidase OS=Cucumis sativus PE=1 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 3.7e-174
Identity = 292/565 (51.68%), Postives = 379/565 (67.08%), Query Frame = 1

Query: 10  YLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQG 69
           +L++ + F F +  S+    +  +YKW+V+    SPDC + + + ING  PGPTI A+ G
Sbjct: 17  FLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGEFPGPTIRANAG 76

Query: 70  DTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTY 129
           D V+VEL N L TE V IHWHGI Q GTPW+DGT  ++QC I PGETF Y+FVVD+ GTY
Sbjct: 77  DIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTYRFVVDKAGTY 136

Query: 130 LYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGS 189
            YH H GMQR  GLYGS++V  PEG++EPF YD + NL+L+DW+H+S H+   GL++   
Sbjct: 137 FYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSVHKQEVGLSS--K 196

Query: 190 GFNWVGEPDSLLIQGRGRFNCSILGSSSG---TCNSSSTE-CVLHPLTVVPGKTYRLRVS 249
              W+GEP S+LI G+G+F+CSI    +     C  S  E C    L V P KTYR+R++
Sbjct: 197 PMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHVQPKKTYRIRIA 256

Query: 250 SLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWIT 309
           S T+L++L+F I  H + VVEADG+YV+PF  +++ IYSGE+YSVLI  DQ+P  NYW++
Sbjct: 257 STTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQNPLENYWVS 316

Query: 310 TNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHT 369
             V +R   TPPGL + NY PN  S+LP + PP  P W D       +  I A  G    
Sbjct: 317 IGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSKNFTFRIFAAMGS-PK 376

Query: 370 PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYD 429
           PP   ++ + LLNTQN +NG  +W++NNVS  +P TPYL A+K  L   F+Q  PP  + 
Sbjct: 377 PPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLNTAFNQNPPPETFP 436

Query: 430 FKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVL 489
             NYDI NP PNPE T  + +Y+ +   TVD+ILQNANM++PN SE HPWHLHGHDFWVL
Sbjct: 437 L-NYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIHPWHLHGHDFWVL 496

Query: 490 GYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMG 549
           GYGEGKF   +D  K NL+NP ++NTV + PYGWTA+RFVADNPGVWAFHCHI+ H  MG
Sbjct: 497 GYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMG 556

Query: 550 MGVVFEEGIERVGKLPTSIMGCGES 571
           MGVVF EG+  VG +P   + CG +
Sbjct: 557 MGVVFAEGVHMVGMIPPKALACGST 577

BLAST of Csa6G500640 vs. Swiss-Prot
Match: ASO_CUCMA (L-ascorbate oxidase OS=Cucurbita maxima GN=AAO PE=1 SV=2)

HSP 1 Score: 605.1 bits (1559), Expect = 7.8e-172
Identity = 291/568 (51.23%), Postives = 383/568 (67.43%), Query Frame = 1

Query: 12  LILLSFVFFLLQSQTAE-SRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGD 71
           LIL  F   L    ++E S+  +YKWEV+    +PDC + + + ING+ PGPTI A+ GD
Sbjct: 13  LILFFFGLILAFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIRANAGD 72

Query: 72  TVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYL 131
           TV+VEL N L TE V IHWHGI Q GTPW+DGT  ++QC I PGETF Y F VD PGT+ 
Sbjct: 73  TVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFF 132

Query: 132 YHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSG 191
           YH H GMQR  GLYGS++V  P+G+ EPF YD + NL+L+DW+H+S H+   GL++    
Sbjct: 133 YHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSS--KP 192

Query: 192 FNWVGEPDSLLIQGRGRFNCSILG---SSSGTCNSSSTE-CVLHPLTVVPGKTYRLRVSS 251
             W+GEP ++L+ GRG+F+CSI     S+   C    +E C  +   V+P KTYR+R++S
Sbjct: 193 IRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMPKKTYRIRIAS 252

Query: 252 LTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITT 311
            T+L+AL+F I  H + VVEADG+YV+PF  +++ IYSGE+YSVLI  DQ+PS NYW++ 
Sbjct: 253 TTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSV 312

Query: 312 NVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTP 371
               R   TPPGL + NY PN  S+LP + PP  PAW+D       +  I A  G    P
Sbjct: 313 GTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITAAMG-SPKP 372

Query: 372 PKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDF 431
           P  +++ I LLNTQN +NG  +W++N+VS  +P TPYL A+K NLLH F Q  PP  +  
Sbjct: 373 PVKSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNPPPEVFP- 432

Query: 432 KNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLG 491
           ++YDI  P  N +  + + +Y+      VD+ILQNANMM  N SE HPWHLHGHDFWVLG
Sbjct: 433 EDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVLG 492

Query: 492 YGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGM 551
           YG+GKF   ++ S  NL+NP ++NTV + PYGWTA+RFVADNPGVWAFHCHI+ H  MGM
Sbjct: 493 YGDGKFTA-EEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGM 552

Query: 552 GVVFEEGIERVGKLPTSIMGCGESKRFL 575
           GVVF EG+E+VG++PT  + CG + + L
Sbjct: 553 GVVFAEGVEKVGRIPTKALACGGTAKSL 575

BLAST of Csa6G500640 vs. Swiss-Prot
Match: ASO_CUCPM (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo PE=1 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 3.9e-171
Identity = 284/550 (51.64%), Postives = 376/550 (68.36%), Query Frame = 1

Query: 29  SRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTENVAIH 88
           S+  +YKWEV+    +P+C + + + ING+ PGPTI A+ GD+V+VEL N L TE V IH
Sbjct: 1   SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIH 60

Query: 89  WHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIV 148
           WHGI Q GTPW+DGT  ++QC I PGETF Y F VD PGT+ YH H GMQR  GLYGS++
Sbjct: 61  WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 120

Query: 149 VWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQGRGRF 208
           V  P+G+ EPF YD + NL+L+DW+H+S H+   GL++      W+GEP ++L+ GRG+F
Sbjct: 121 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSS--KPIRWIGEPQTILLNGRGQF 180

Query: 209 NCSILG---SSSGTCNSSSTE-CVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTV 268
           +CSI     S+   C    +E C  +   V P KTYR+R++S T+L+AL+F I  H + V
Sbjct: 181 DCSIAAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLV 240

Query: 269 VEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNY 328
           VEADG+YV+PF  +++ IYSGE+YSVLI  DQ+PS NYW++    +R   TPPGL + NY
Sbjct: 241 VEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNY 300

Query: 329 YPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQNTVN 388
            PN  S+LP + PP  PAW+D       +  I A  G    PP   ++ I LLNTQN +N
Sbjct: 301 LPNSVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMG-SPKPPVKFNRRIFLLNTQNVIN 360

Query: 389 GRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSD 448
           G  +W++N+VS  +P TPYL A+K+NLLH F Q  PP  +  ++YDI  P  N +  + +
Sbjct: 361 GYVKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFP-EDYDIDTPPTNEKTRIGN 420

Query: 449 AIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYNLE 508
            +Y+      VD+ILQNANMM  N SETHPWHLHGHDFWVLGYG+GKF   ++ S  NL+
Sbjct: 421 GVYQFKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSA-EEESSLNLK 480

Query: 509 NPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSI 568
           NP ++NTV + PYGWTA+RFVADNPGVWAFHCHI+ H  MGMGVVF EG+E+VG++PT  
Sbjct: 481 NPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKA 540

Query: 569 MGCGESKRFL 575
           + CG + + L
Sbjct: 541 LACGGTAKSL 545

BLAST of Csa6G500640 vs. Swiss-Prot
Match: ASO_TOBAC (L-ascorbate oxidase OS=Nicotiana tabacum GN=AAO PE=2 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 1.7e-166
Identity = 292/578 (50.52%), Postives = 378/578 (65.40%), Query Frame = 1

Query: 15  LSFVFF------LLQSQTAES----RTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTI 74
           L F+FF      LL+  ++ S    +T ++KW+V+    SPD  + V + ING+ PGPTI
Sbjct: 4   LGFLFFFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTI 63

Query: 75  YAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVD 134
            A  GDTV V L N L TE V IHWHGIRQIGTPW+DGT  ++QC I PGETF Y+F VD
Sbjct: 64  RAKAGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVD 123

Query: 135 RPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGL 194
           + GTY YH HYGMQR  GLYGS++V V EG+ EPF YD + NL+L+DW+HK SHE    L
Sbjct: 124 KAGTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDL 183

Query: 195 ATPGSGFNWVGEPDSLLIQGRGRFNCSILGSSSGTCNSSSTECVLHP--------LTVVP 254
           ++  +   W+GEP +LL+ GRG++NCS+    S        +C L          L V P
Sbjct: 184 SS--NPLRWIGEPQTLLLNGRGQYNCSLAARFS---KPPLPQCKLRGGEQYAPQILRVRP 243

Query: 255 GKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQ 314
            K YRLRV+S T+L +LS  I GH M VVEADG+YV+PF V ++ IYSGE+YSVL K DQ
Sbjct: 244 NKIYRLRVASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQ 303

Query: 315 DPSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAI 374
           DP++NYWI+ NV  R   TP GL + NY PN  S+ P   PP  P WND     + S  I
Sbjct: 304 DPTKNYWISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKI 363

Query: 375 KARQGFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFS 434
            A  G    PP+   + I+LLNTQN ++G  +W++NNVS  +P   YL ++++  ++ F 
Sbjct: 364 FALMGSPKPPPQ-NHRRIILLNTQNKIDGYTKWAINNVSLVLPTQLYLGSIRYG-INAFD 423

Query: 435 QEKPPTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWH 494
            + PP  +  K+YD+   APN  +T  + +Y L FN+T+DIILQNAN ++ + SE HPWH
Sbjct: 424 TKPPPDNFP-KDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWH 483

Query: 495 LHGHDFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHC 554
           LHGHDFWVLGYGEGKF   KD  K+NL+NP ++NT  + P+GWTALRFV DNPGVWAFHC
Sbjct: 484 LHGHDFWVLGYGEGKFS-EKDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHC 543

Query: 555 HIDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFL 575
           HI+ H  MGMGV+F EG+  V K+P   + CG + + L
Sbjct: 544 HIEPHLHMGMGVIFAEGVHLVKKIPKEALACGLTGKML 572

BLAST of Csa6G500640 vs. Swiss-Prot
Match: LAC22_ORYSJ (Laccase-22 OS=Oryza sativa subsp. japonica GN=LAC22 PE=2 SV=2)

HSP 1 Score: 289.7 bits (740), Expect = 7.3e-77
Identity = 186/559 (33.27%), Postives = 287/559 (51.34%), Query Frame = 1

Query: 5   LQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTI 64
           L  +R L +LL    FLLQ+ +A + T +YK+ V     +  C  K  LT+NG+ PGPT+
Sbjct: 4   LPESRRLSLLLMAACFLLQALSAHAITRHYKFNVVMRNMTRLCSTKPILTVNGKFPGPTL 63

Query: 65  YAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVD 124
           YA +GD V+V++ N +   NV IHWHG+RQI T W DG   +TQCPI PG +F Y F + 
Sbjct: 64  YAREGDNVLVKVVNHV-AHNVTIHWHGVRQIRTGWYDGPAYITQCPIQPGSSFLYNFTIT 123

Query: 125 -RPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPF-SYDLDRNLILTDWYHKSSHEHAT 184
            + GT L+HAH    R   ++G+IV+    G   PF +   +  ++L +W+ + +     
Sbjct: 124 GQRGTLLWHAHINWLRAT-VHGAIVILPKLGVPYPFPAPHKEAVIVLGEWWKEDTETVIN 183

Query: 185 GLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTYRL 244
                G G N     DS  I G          S  G             L+V  GKTY L
Sbjct: 184 QAMQLGVGPNI---SDSHTINGHPGPLSECASSQDG-----------FKLSVENGKTYML 243

Query: 245 RVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR-- 304
           R+ +      L F++ GH +TVVE D  Y +PF+ + L I  G+T +VL++A+Q   R  
Sbjct: 244 RIINAALNDDLFFKVAGHELTVVEVDAVYTKPFKTDTLLITPGQTTNVLVRANQGAGRYL 303

Query: 305 ---NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIK 364
              + ++   V   ++T    L   N   +  + L    PP   A + V+       ++ 
Sbjct: 304 LSVSPFMDAPVQVDNKTGTATLHYANTVSSSMASLTLVKPPPQNATHIVSKFTDSLHSLN 363

Query: 365 ARQGFIHTPPKAADKVIVLLNT-----QNTVNGRRR-WSLNNVSFNMPHTPYLIALKHNL 424
           +++   + P      +++ +        + +NG R   ++NNV+F MP TP L A  +N+
Sbjct: 364 SKEYPANVPQTVDHSLLLTVGVGVNPCPSCINGTRVVGTINNVTFIMPSTPILQAHYYNI 423

Query: 425 LHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSE 484
              F+++ P T     NY    P  N +      +YRL +N++V ++LQ+  ++SP   E
Sbjct: 424 PGVFTEDFPATPLHKFNYTGSGPK-NLQTMNGTRVYRLPYNASVQVVLQDTGIISP---E 483

Query: 485 THPWHLHGHDFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGV 544
           +HP HLHG +F+V+G G G ++    PS +NL +PI +NT+ +   GWTA+RF +DNPGV
Sbjct: 484 SHPIHLHGFNFFVVGKGVGNYNPRTSPSTFNLIDPIERNTIGVPTGGWTAIRFRSDNPGV 542

Query: 545 WAFHCHIDAHFFMGMGVVF 551
           W  HCH + H   G+ + F
Sbjct: 544 WFMHCHFEVHTSWGLKMAF 542

BLAST of Csa6G500640 vs. TrEMBL
Match: A0A0A0KK62_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G500640 PE=3 SV=1)

HSP 1 Score: 1211.8 bits (3134), Expect = 0.0e+00
Identity = 576/576 (100.00%), Postives = 576/576 (100.00%), Query Frame = 1

Query: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60
           MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP
Sbjct: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180

Query: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTY 240
           ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTY
Sbjct: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300
           RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300

Query: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ 360
           NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ
Sbjct: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ 360

Query: 361 GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420
           GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP
Sbjct: 361 GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420

Query: 421 PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480
           PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH
Sbjct: 421 PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP
Sbjct: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

BLAST of Csa6G500640 vs. TrEMBL
Match: U5G9V4_POPTR (L-ascorbate oxidase family protein OS=Populus trichocarpa GN=POPTR_0007s05890g PE=3 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 2.9e-258
Identity = 421/565 (74.51%), Postives = 475/565 (84.07%), Query Frame = 1

Query: 14  LLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVI 73
           L  FV  L+    A++R  +YKWE+K E KSPDC+KK+ +TINGR+PGPTI A Q DTVI
Sbjct: 6   LCFFVISLINIPIADARIRHYKWELKYEYKSPDCYKKLVITINGRTPGPTILAKQNDTVI 65

Query: 74  VELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHA 133
           VE+KNSL TEN AIHWHGIRQIGTPW DGTEGVTQCPILPG+TF Y+FVVDRPGTYLYHA
Sbjct: 66  VEVKNSLLTENTAIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFVYKFVVDRPGTYLYHA 125

Query: 134 HYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNW 193
           HYGMQRE G+YGSI V +P+G++EPF+YD DR++ILTDWYHKS++E A GL++    F W
Sbjct: 126 HYGMQREAGIYGSIRVALPDGESEPFAYDYDRSIILTDWYHKSTYEQAAGLSS--IPFQW 185

Query: 194 VGEPDSLLIQGRGRFNCSILGS--SSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLS 253
           VGEP SLLIQG+GRF+CS       +  CN+++ EC L+  TVVPGKTYRLR+SSL++LS
Sbjct: 186 VGEPQSLLIQGKGRFDCSAANPPLKADVCNNTNPECSLYSTTVVPGKTYRLRISSLSALS 245

Query: 254 ALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSR 313
           ALSFQIEGH MTVVEADGHYVEPF V NLFIYSGETYSVL+K DQDPSRNYW TTNVVSR
Sbjct: 246 ALSFQIEGHNMTVVEADGHYVEPFVVKNLFIYSGETYSVLVKTDQDPSRNYWATTNVVSR 305

Query: 314 SRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAAD 373
           + TTPPGLAIFNYYPNH  R PPT PP+ P WND+ PR  QS AIKAR+G I+ PP  +D
Sbjct: 306 NATTPPGLAIFNYYPNHPRRSPPTIPPSGPMWNDIAPRFNQSVAIKARRGHIYPPPATSD 365

Query: 374 KVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDI 433
           +VIVLLNTQNTVNG  RWS+N VSFN+PHTPYLIALK NLLHTFSQ  PP GYDFKNYDI
Sbjct: 366 RVIVLLNTQNTVNGNVRWSVNKVSFNIPHTPYLIALKENLLHTFSQTPPPEGYDFKNYDI 425

Query: 434 FNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGK 493
           F    N  AT SDAIYRL  NSTVDIILQNAN M+PNNSETHPWHLHGHDFWVLGYG+GK
Sbjct: 426 FARQNNTNATTSDAIYRLQLNSTVDIILQNANTMNPNNSETHPWHLHGHDFWVLGYGKGK 485

Query: 494 FDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFE 553
           FD   DP  YNL +PIMKNTVP+HP+GWTALRF ADNPGVWAFHCHI++HFFMGMGVVFE
Sbjct: 486 FDPINDPKNYNLVDPIMKNTVPVHPFGWTALRFKADNPGVWAFHCHIESHFFMGMGVVFE 545

Query: 554 EGIERVGKLPTSIMGCGESKRFLRP 577
           EGIERVGKLP+SIMGCGE+KR L+P
Sbjct: 546 EGIERVGKLPSSIMGCGETKRLLKP 568

BLAST of Csa6G500640 vs. TrEMBL
Match: B9HFP2_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0007s05910g PE=3 SV=2)

HSP 1 Score: 897.1 bits (2317), Expect = 1.1e-257
Identity = 421/567 (74.25%), Postives = 476/567 (83.95%), Query Frame = 1

Query: 12  LILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDT 71
           L L  FV  L+    AE+R  +YK+E+K E KSPDC+KK+ +TINGR+PGPTI+A Q DT
Sbjct: 18  LALCFFVISLINIPIAEARIRHYKFELKYEYKSPDCYKKLVITINGRTPGPTIFAQQNDT 77

Query: 72  VIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLY 131
           VIVE+KNSL TEN AIHWHGIRQIGTPW DGTEGVTQCPILPG+TF Y+FVVDRPGTYLY
Sbjct: 78  VIVEVKNSLLTENTAIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFVYKFVVDRPGTYLY 137

Query: 132 HAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGF 191
           HAHYGMQRE G+YGSI V +P+G++EPF+YD DR++ILTDWYHKS++E ATGL++    F
Sbjct: 138 HAHYGMQREAGIYGSIRVALPDGESEPFAYDYDRSIILTDWYHKSTYEQATGLSS--IPF 197

Query: 192 NWVGEPDSLLIQGRGRFNCSILGS--SSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTS 251
            WVGEP SLLIQG+GRF+CS       +  CN++S EC L+  TVVPGKTYRLR+SSLT+
Sbjct: 198 QWVGEPQSLLIQGKGRFDCSAANPPLKADVCNNTSPECSLYSTTVVPGKTYRLRISSLTA 257

Query: 252 LSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVV 311
           LSALSFQIEGH++TVVEADGHYVEPF V NLFIYSGETYSVL+K DQDPSRNYW TTNVV
Sbjct: 258 LSALSFQIEGHSLTVVEADGHYVEPFVVKNLFIYSGETYSVLVKTDQDPSRNYWATTNVV 317

Query: 312 SRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKA 371
           SR+ TTPPGLAIFNYYPNH  R PPT PP+ P WND+ PR  QS AIKAR+G I++PP  
Sbjct: 318 SRNATTPPGLAIFNYYPNHPRRSPPTIPPSGPMWNDIEPRFNQSVAIKARKGHIYSPPAT 377

Query: 372 ADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNY 431
           +D+VIV LNTQN VNG  RWS+NNVSFN+PHTPYLIALK NLLHTFSQ  PP GYDFKNY
Sbjct: 378 SDRVIVFLNTQNRVNGNVRWSVNNVSFNIPHTPYLIALKENLLHTFSQTPPPEGYDFKNY 437

Query: 432 DIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGE 491
           DIF    N  AT SDAIYRL  NSTVDIILQNAN M+PNNSETHPWHLHGHDFWVLGYG+
Sbjct: 438 DIFARQNNTNATTSDAIYRLQLNSTVDIILQNANTMNPNNSETHPWHLHGHDFWVLGYGK 497

Query: 492 GKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVV 551
           GKFD   DP  YNL +PIMKNTVP+HP GWTALRF ADNPG WAFHCHI++HFFMGMGVV
Sbjct: 498 GKFDPINDPKNYNLVDPIMKNTVPVHPLGWTALRFKADNPGAWAFHCHIESHFFMGMGVV 557

Query: 552 FEEGIERVGKLPTSIMGCGESKRFLRP 577
           FEEGIERVGKLP+SIMGCGE+K  L+P
Sbjct: 558 FEEGIERVGKLPSSIMGCGETKHLLKP 582

BLAST of Csa6G500640 vs. TrEMBL
Match: A0A061DIV4_THECC (Cupredoxin superfamily protein isoform 1 OS=Theobroma cacao GN=TCM_001179 PE=3 SV=1)

HSP 1 Score: 890.6 bits (2300), Expect = 1.0e-255
Identity = 416/579 (71.85%), Postives = 472/579 (81.52%), Query Frame = 1

Query: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60
           + S  +S   LL+ LS +  LL     E+R   YKWEVK E +SPDC KK+ +TING++P
Sbjct: 3   LLSECRSRVILLLSLSVIIGLLNGSGVEARIRRYKWEVKYEYRSPDCLKKLVITINGQTP 62

Query: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTI A Q DT+IVEL N+L TENVAIHWHGIRQIGTPW DGTEGVTQCPILPG TF+YQ
Sbjct: 63  GPTIRAQQNDTIIVELTNNLSTENVAIHWHGIRQIGTPWFDGTEGVTQCPILPGTTFKYQ 122

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQRE GLYGSI+V +P+GQ+EPF+YD DRN+IL DWYHKS++E 
Sbjct: 123 FVVDRPGTYLYHAHYGMQREAGLYGSIIVALPDGQSEPFTYDYDRNIILNDWYHKSTYEQ 182

Query: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILG---SSSGTCNSSSTECVLHPLTVVPG 240
           A GL+     F WVGEP SLLI GRGRFNCS L    S +G CN+++ +C    L VVPG
Sbjct: 183 AAGLSA--IPFQWVGEPQSLLIHGRGRFNCSSLTIPTSDTGVCNTTNPDCAPFRLIVVPG 242

Query: 241 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300
           KTYRLR+SSLT+LSALSFQIEGH MTVVEADGHYVEPF V NLFIYSGETYSVL+KADQD
Sbjct: 243 KTYRLRISSLTALSALSFQIEGHNMTVVEADGHYVEPFVVQNLFIYSGETYSVLVKADQD 302

Query: 301 PSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIK 360
           P+RNYWIT+N+VSR   T PGLAIFNYYPNH  R PPT+PPA P WN+  PR+AQSQAIK
Sbjct: 303 PTRNYWITSNIVSRPANTTPGLAIFNYYPNHPRRSPPTSPPAAPVWNNSRPRIAQSQAIK 362

Query: 361 ARQGFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420
           AR G+IH PP  +D+VIV LNTQN +NG  RWS+NNVSF +PHTPYLIALK NL H F Q
Sbjct: 363 ARPGYIHPPPLLSDRVIVFLNTQNRINGNVRWSVNNVSFTLPHTPYLIALKENLTHAFDQ 422

Query: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHL 480
             PP GYDF NYDIF    N  AT+ + IYRL+FNSTVDIILQNAN M+ NNSETHPWHL
Sbjct: 423 NPPPDGYDFVNYDIFKEQENKNATLGNGIYRLNFNSTVDIILQNANTMTKNNSETHPWHL 482

Query: 481 HGHDFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540
           HGHDFWVLGYGEGKFD++ DP KYNL NPIMKNTVP+HPYGWTALRF ADNPG WAFHCH
Sbjct: 483 HGHDFWVLGYGEGKFDMFNDPRKYNLVNPIMKNTVPVHPYGWTALRFKADNPGAWAFHCH 542

Query: 541 IDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           I++HF+MGMGVVF EGIE+VG LP+SIMGCGE++   RP
Sbjct: 543 IESHFYMGMGVVFAEGIEKVGNLPSSIMGCGETQGLYRP 579

BLAST of Csa6G500640 vs. TrEMBL
Match: K4BSL7_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=3 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 2.2e-253
Identity = 410/565 (72.57%), Postives = 474/565 (83.89%), Query Frame = 1

Query: 12  LILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDT 71
           L++L  + FL  S + E+R  +Y+WEVK E KSPDCFKK++++ING++PGPTI A QGDT
Sbjct: 16  LVVLVCLLFLSASISVEARIRHYQWEVKYEYKSPDCFKKLSISINGKTPGPTIVAQQGDT 75

Query: 72  VIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLY 131
           V+VE+KNSL TEN+AIHWHGIRQIGTPW+DGTEGVTQCPI+PG+TF Y+FVVDR GTYLY
Sbjct: 76  VVVEVKNSLLTENLAIHWHGIRQIGTPWADGTEGVTQCPIVPGDTFIYKFVVDRAGTYLY 135

Query: 132 HAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGF 191
           HAHYGMQR+ GL G + V +P+G  EPFSYD DR+++LTDWYHKS++E ATGLA+    F
Sbjct: 136 HAHYGMQRQAGLQGMMRVSLPDGVLEPFSYDHDRSILLTDWYHKSTYEQATGLAS--LPF 195

Query: 192 NWVGEPDSLLIQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLS 251
           +WVGEP S+LI GRGRFNCSI       CN+++ +C  + +TVVPGKTYRLR+ SLT+LS
Sbjct: 196 SWVGEPQSILIHGRGRFNCSIPTIDPTLCNATNPQCTPYSMTVVPGKTYRLRIGSLTALS 255

Query: 252 ALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSR 311
           ALSF+IEGH MTVVEADGHYVEPF V NLFIYSGETYSVLIKADQDP+RNYW +T VVSR
Sbjct: 256 ALSFEIEGHNMTVVEADGHYVEPFVVKNLFIYSGETYSVLIKADQDPTRNYWASTKVVSR 315

Query: 312 SRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAAD 371
           + TTP GL IFNYYPNH  R PPT PP+ P W+DV PR+AQS AIK+ + FIH PP  +D
Sbjct: 316 NNTTPNGLGIFNYYPNHPRRYPPTVPPSGPRWDDVAPRMAQSVAIKSHRDFIHAPPLTSD 375

Query: 372 KVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDI 431
           +VIV+LNTQN VNG  RWS+NNVSFNMPHTPYLIALKHNLLH F Q  PP  YD KNYDI
Sbjct: 376 RVIVMLNTQNRVNGYVRWSVNNVSFNMPHTPYLIALKHNLLHAFEQTPPPDNYDQKNYDI 435

Query: 432 FNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGK 491
           FN APN  AT S++IYRL FN+TVDIILQNAN M+ NNSETHPWHLHGHDFWV+GYG GK
Sbjct: 436 FNIAPNVNATTSNSIYRLKFNTTVDIILQNANTMNLNNSETHPWHLHGHDFWVMGYGNGK 495

Query: 492 FDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFE 551
           F+   DP  YN  NPIMKNT+P+HPYGWTALRF ADNPGVWAFHCHI++HFFMGMGVVFE
Sbjct: 496 FNQSIDPKNYNFVNPIMKNTIPVHPYGWTALRFRADNPGVWAFHCHIESHFFMGMGVVFE 555

Query: 552 EGIERVGKLPTSIMGCGESKRFLRP 577
           EGIE+VGKLPTSIMGCGESKRF RP
Sbjct: 556 EGIEKVGKLPTSIMGCGESKRFHRP 578

BLAST of Csa6G500640 vs. TAIR10
Match: AT4G39830.1 (AT4G39830.1 Cupredoxin superfamily protein)

HSP 1 Score: 803.5 bits (2074), Expect = 8.4e-233
Identity = 382/569 (67.14%), Postives = 443/569 (77.86%), Query Frame = 1

Query: 12  LILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDT 71
           L++L F+     S   + +   +KWEVK E KSPDCF+K+ +TING+ PGPTI A QGDT
Sbjct: 16  LMVLCFIALFFSSVLCQGKIRRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQGDT 75

Query: 72  VIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLY 131
           ++VELKNS  TENVA+HWHGIRQIGTPW DG EGVTQCPILPGE F YQFVVDRPGTY+Y
Sbjct: 76  IVVELKNSFMTENVAVHWHGIRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGTYMY 135

Query: 132 HAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGF 191
           H+HYGMQRE GL G I V  P  + EPF+YD DRN +LTDWYHKS  E ATGLA+    F
Sbjct: 136 HSHYGMQRESGLIGMIQVSPPATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLAS--IPF 195

Query: 192 NWVGEPDSLLIQGRGRFNCSILGSS-----SGTCNSSSTECVLHPLTVVPGKTYRLRVSS 251
            WVGEP SL+IQGRGRFNCS   ++     SG CN S+ +C    LTV+PGKTYRLR+ S
Sbjct: 196 KWVGEPQSLMIQGRGRFNCSNNLTTPPSLVSGVCNVSNADCSRFILTVIPGKTYRLRIGS 255

Query: 252 LTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITT 311
           LT+LSALSFQIEGH +TVVEADGHYVEPF V NLF+YSGETYSVL+KADQ+P RNYWIT+
Sbjct: 256 LTALSALSFQIEGHNLTVVEADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWITS 315

Query: 312 NVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPA--PPAWNDVTPRLAQSQAIKARQGFIH 371
           ++VSR  TTPP  A+ NYYPNH  R PPT+  +   P WND   RLAQS AIKAR+GFIH
Sbjct: 316 SIVSRPATTPPATAVLNYYPNHPRRRPPTSESSNIVPEWNDTRSRLAQSLAIKARRGFIH 375

Query: 372 TPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFS-QEKPPTG 431
             P+ +DKVIVLLNTQN VNG RRWS+NNVS++ P TPYLIALK NL + F  +   P  
Sbjct: 376 ALPENSDKVIVLLNTQNEVNGYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTAPEN 435

Query: 432 YDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFW 491
           YD +NYDIF    N  AT SD IYRL FNSTVD+ILQNAN M+ NNSETHPWHLHGHDFW
Sbjct: 436 YDSRNYDIFAKPLNANATTSDGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGHDFW 495

Query: 492 VLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFF 551
           VLGYGEGKF+  +DP +YN  +PI KNTV + P+GWTALRF ADNPGVW+FHCHI++HFF
Sbjct: 496 VLGYGEGKFNESEDPKRYNRVDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCHIESHFF 555

Query: 552 MGMGVVFEEGIERVGKLPTSIMGCGESKR 573
           MGMG+VFE GI++V  LP+SIMGCG++KR
Sbjct: 556 MGMGIVFESGIDKVSSLPSSIMGCGQTKR 582

BLAST of Csa6G500640 vs. TAIR10
Match: AT5G21105.1 (AT5G21105.1 Plant L-ascorbate oxidase)

HSP 1 Score: 584.7 bits (1506), Expect = 6.1e-167
Identity = 277/557 (49.73%), Postives = 366/557 (65.71%), Query Frame = 1

Query: 24  SQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTE 83
           + TA +    Y WEV+ +  SPDC +   +T+NG  PGPTI A  GDT++V L N L TE
Sbjct: 30  THTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTE 89

Query: 84  NVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGL 143
            + IHWHGIRQ G+PW+DG  GVTQC I PGETF Y F V++PGT+ YH HYGMQR  GL
Sbjct: 90  GLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAGL 149

Query: 144 YGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQ 203
           YGS++V V +G++E   YD + NL+L+DW+H++      GL++      W+GE  S+LI 
Sbjct: 150 YGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSS--KPMRWIGEAQSILIN 209

Query: 204 GRGRFNCSILGSSSGTCN------SSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQI 263
           GRG+FNCS+    S   +          +C    L V P KTYR+R+SS T+L++L+  +
Sbjct: 210 GRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAV 269

Query: 264 EGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPP 323
           +GH + VVEADG+Y+ PF  +++ IYSGE+YSVL+  DQDPS+NY+I+  V  R   T  
Sbjct: 270 QGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQ 329

Query: 324 GLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLL 383
            L I NY     S+LP + PP  P W+D       S+ I +  G   +PPK   K ++LL
Sbjct: 330 ALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGS-PSPPKKYRKRLILL 389

Query: 384 NTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPN 443
           NTQN ++G  +W++NNVS   P TPYL ++K+NL   F+++ PP  Y   +YDI NP P 
Sbjct: 390 NTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM-DYDIMNPPPF 449

Query: 444 PEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKD 503
           P  T  + IY   FN TVD+I+QNAN++    SE HPWHLHGHDFWVLGYG+GKF    D
Sbjct: 450 PNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGID 509

Query: 504 PSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERV 563
              YNL+NP ++NT  L+PYGWTA+RFV DNPGVW FHCHI+ H  MGMGVVF EG+ R+
Sbjct: 510 EKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRI 569

Query: 564 GKLPTSIMGCGESKRFL 575
           GK+P   +GCG +K+FL
Sbjct: 570 GKVPDEALGCGLTKQFL 582

BLAST of Csa6G500640 vs. TAIR10
Match: AT5G21100.1 (AT5G21100.1 Plant L-ascorbate oxidase)

HSP 1 Score: 562.8 bits (1449), Expect = 2.5e-160
Identity = 273/557 (49.01%), Postives = 358/557 (64.27%), Query Frame = 1

Query: 26  TAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVIVELKNSLFTENV 85
           +A +  +   WEV+ +   PDC + + + ING+ PGPTI A  GDTVI+ + N L TE V
Sbjct: 18  SASAAVVESTWEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVAGDTVIIHVVNKLSTEGV 77

Query: 86  AIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYG 145
            IHWHGIRQ GTPW+DG  GVTQCPI PGETF Y+F+VD+ GT+ YH HYGMQR  GLYG
Sbjct: 78  VIHWHGIRQKGTPWADGAAGVTQCPINPGETFTYKFIVDKAGTHFYHGHYGMQRSSGLYG 137

Query: 146 SIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQGR 205
            ++V  P+   E   YD + NL+L+DW+H+S H  A  LA       W+GEP SLLI GR
Sbjct: 138 MLIVRSPK---ERLIYDGEFNLLLSDWWHQSIH--AQELALSSRPMRWIGEPQSLLINGR 197

Query: 206 GRFNCSIL-----GSSSGTCN-SSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEG 265
           G+FNCS       G     C    + +C    L V P + YRLR++S T+L++L+  ++G
Sbjct: 198 GQFNCSQAAYFNKGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRIASTTALASLNLAVQG 257

Query: 266 HTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGL 325
           H + VVEADG+YV PF VN++ +YSGETYSVL+K +  PS+ YWI+  V  R   TP  L
Sbjct: 258 HQLVVVEADGNYVAPFTVNDIDVYSGETYSVLLKTNALPSKKYWISVGVRGREPKTPQAL 317

Query: 326 AIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNT 385
            + NY    +SR P   PP  P WND     + S+ I A +G+   P K+ D+ ++LLNT
Sbjct: 318 TVINYVDATESR-PSHPPPVTPIWNDTDRSKSFSKKIFAAKGYPKPPEKSHDQ-LILLNT 377

Query: 386 QNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPE 445
           QN      +WS+NNVS ++P TPYL ++++ L   +  + P       NYDI  P PNP 
Sbjct: 378 QNLYEDYTKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKKLIMDNYDIMKPPPNPN 437

Query: 446 ATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPS 505
            T    IY   F   VD+ILQNAN++    SE HPWH+HGHDFWVLGYGEGKF    D  
Sbjct: 438 TTKGSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGHDFWVLGYGEGKFKPGIDEK 497

Query: 506 KYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGK 565
            +NL+NP ++NTV L+P+GWTA+RFV DNPGVW FHCHI+ H  MGMGVVF EG++R+GK
Sbjct: 498 TFNLKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFVEGVDRIGK 557

Query: 566 --LPTSIMGCGESKRFL 575
             +P   +GCG ++++L
Sbjct: 558 MEIPDEALGCGLTRKWL 567

BLAST of Csa6G500640 vs. TAIR10
Match: AT5G09360.1 (AT5G09360.1 laccase 14)

HSP 1 Score: 278.9 bits (712), Expect = 7.2e-75
Identity = 191/565 (33.81%), Postives = 294/565 (52.04%), Query Frame = 1

Query: 11  LLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGD 70
           L  ++ F+F LL  Q AE+   ++ +++KS+  +  C     LT+NG  PGPT+ A++GD
Sbjct: 15  LQTIVFFLFVLLAFQIAEAEIHHHTFKIKSKAYTRLCNTNKILTVNGEFPGPTLKAYRGD 74

Query: 71  TVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVD-RPGTY 130
            +IV + N+    N+ +HWHG RQI  PWSDG E VTQCPI PGE++ Y+  +    GT 
Sbjct: 75  KLIVNVINNA-NYNITLHWHGARQIRNPWSDGPEYVTQCPIRPGESYVYRIDLKVEEGTI 134

Query: 131 LYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYD-LDRNLILTDWYHKSSHEHATGLATPG 190
            +HAH    R   ++G+ +V+   G + PF     +  LIL +W+ K +  H      PG
Sbjct: 135 WWHAHSQWARAT-VHGAFIVYPKRGSSYPFPKPHREIPLILGEWWKKENIMHI-----PG 194

Query: 191 SGFNWVGEP---DSLLIQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTYRLRVS 250
                 GEP   DS  I G+  +           C+   T      +TVV G+ Y LR+ 
Sbjct: 195 KANKTGGEPAISDSYTINGQPGYLYP--------CSKPET----FKITVVRGRRYLLRII 254

Query: 251 SLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWIT 310
           +      L F I  HT+TVV  DG Y++ F+ + L I  G++  VL+ A+Q P+ +Y++ 
Sbjct: 255 NAVMDEELFFAIANHTLTVVAKDGFYLKHFKSDYLMITPGQSMDVLLHANQRPN-HYFVA 314

Query: 311 TNVVSRS------RTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKAR 370
               S +      +TT    AI  Y  +  +R+ P  P  PP +N        +   +++
Sbjct: 315 ARAYSSAFGAGFDKTTTT--AILQYKGDTLNRIKPILPYLPP-YNRTEASTRFTNQFRSQ 374

Query: 371 QGFIHTPPKAADKVI--VLLNTQNTVNGR-------RRWS--LNNVSFNMPHTPYLIALK 430
           +  ++ P K   +++  + +N  N  + R       +R+S  +NN+SF  P    L A  
Sbjct: 375 RP-VNVPVKINTRLLYAISVNLMNCSDDRPCTGPFGKRFSSSINNISFVNPSVDILRAYY 434

Query: 431 HNLLHTFSQE---KPPTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMM 490
            ++   F ++    PPT +++   ++  P P    T    +  LD+NS+V++ILQ   + 
Sbjct: 435 RHIGGVFQEDFPRNPPTKFNYTGENL--PFPTRFGT---KVVVLDYNSSVELILQGTTVW 494

Query: 491 SPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFV 550
           + N    HP HLHG++F+V+G G G FD  KDP +YNL +P  + TV +   GWTA+RFV
Sbjct: 495 ASN---IHPIHLHGYNFYVVGSGFGNFDRRKDPLRYNLVDPPEETTVGVPRNGWTAVRFV 547

BLAST of Csa6G500640 vs. TAIR10
Match: AT2G40370.1 (AT2G40370.1 laccase 5)

HSP 1 Score: 275.0 bits (702), Expect = 1.0e-73
Identity = 186/580 (32.07%), Postives = 286/580 (49.31%), Query Frame = 1

Query: 8   TRYLLILLSFVFFLLQSQTAES-RTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYA 67
           T+ LL  +SFV FLL S  AE+ +  ++++ +++      C    ++T+NG  PGP +  
Sbjct: 4   TKSLLCFISFVAFLLFSSVAEANKAHHHEFIIQATKVKRLCETHNSITVNGMFPGPMLVV 63

Query: 68  HQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVD-R 127
           + GDT++V++ N     N+ IHWHG+RQ+ T W+DG E VTQCPI PG ++ Y+F +  +
Sbjct: 64  NNGDTLVVKVINRA-RYNITIHWHGVRQMRTGWADGPEFVTQCPIRPGSSYTYRFTIQGQ 123

Query: 128 PGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRN--LILTDWYHKSSHEHATG 187
            GT  +HAH    R   +YGS++V+ P G + PF+    RN  L+L +W+  +  +    
Sbjct: 124 EGTLWWHAHSSWLRAT-VYGSLLVFPPAGSSYPFTKP-HRNVPLLLGEWWDANPVDVLRE 183

Query: 188 LATPGSGFNWVGEPDSLLIQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTYRLR 247
               G   N     D+  I G+              C+S  T  V  P+ V  G+T  LR
Sbjct: 184 SIRTGGAPN---NSDAYTINGQP--------GDLYKCSSQDTTVV--PINV--GETILLR 243

Query: 248 VSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYW 307
           V +      L F +  H +TVV AD  Y++PF  N + +  G+T  VLI  DQ P+R Y+
Sbjct: 244 VINSALNQPLFFTVANHKLTVVGADASYLKPFTTNVIVLGPGQTTDVLITGDQPPNR-YY 303

Query: 308 ITTNVVSRSRTTPPG----LAIFNYYP-----------NHQSRLPPTTPPAPPAWNDVTP 367
           +       ++  P G     AI  Y               +        P  PA+ND   
Sbjct: 304 MAARAYQSAQNAPFGNTTTTAILQYKSAPCCGVGGGSGTKKGNSFKPIMPILPAYNDTNT 363

Query: 368 RLAQSQAIKARQGFIHTPPKAADKVIVLL-----NTQNTVNGRR---------RWSLNNV 427
               SQ+ ++ +     P +  + + V +     N       RR           S+NNV
Sbjct: 364 VTRFSQSFRSLRR-AEVPTEIDENLFVTIGLGLNNCPKNFRSRRCQGPNGTRFTASMNNV 423

Query: 428 SFNMPHTPYLIALKHN----LLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIYRLD 487
           SF +P    L+   H+    +  T    KPP  +D+   +I      P+      +Y+L 
Sbjct: 424 SFALPSNYSLLQAHHHGIPGVFTTDFPAKPPVKFDYTGNNISRSLYQPDRGTK--LYKLK 483

Query: 488 FNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYNLENPIMKN 547
           + S V I+LQ+  +++P N   HP HLHG+DF+++  G G F+  KD +K+NLE+P ++N
Sbjct: 484 YGSRVQIVLQDTGIVTPEN---HPIHLHGYDFYIIAEGFGNFNPKKDTAKFNLEDPPLRN 543

Query: 548 TVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVF 551
           TV +   GW  +RF+ADNPGVW  HCH+DAH   G+ + F
Sbjct: 544 TVGVPVNGWAVIRFIADNPGVWIMHCHLDAHISWGLAMAF 558

BLAST of Csa6G500640 vs. NCBI nr
Match: gi|449451465|ref|XP_004143482.1| (PREDICTED: L-ascorbate oxidase [Cucumis sativus])

HSP 1 Score: 1211.8 bits (3134), Expect = 0.0e+00
Identity = 576/576 (100.00%), Postives = 576/576 (100.00%), Query Frame = 1

Query: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60
           MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP
Sbjct: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180

Query: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTY 240
           ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTY
Sbjct: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300
           RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300

Query: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ 360
           NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ
Sbjct: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ 360

Query: 361 GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420
           GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP
Sbjct: 361 GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420

Query: 421 PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480
           PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH
Sbjct: 421 PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP
Sbjct: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

BLAST of Csa6G500640 vs. NCBI nr
Match: gi|659080148|ref|XP_008440636.1| (PREDICTED: L-ascorbate oxidase [Cucumis melo])

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 549/576 (95.31%), Postives = 561/576 (97.40%), Query Frame = 1

Query: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60
           MAS LQ  +Y L+LLS   FLLQSQTAESRTLNYKWEVKSELKSPDCFKK+T+TINGRSP
Sbjct: 1   MASLLQRKQYFLLLLSSFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNSL TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYD DRNLILTDWYHKSS+EH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRNLILTDWYHKSSNEH 180

Query: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTY 240
           A GLATPGSGF WVGEPDSLLIQGRGRFNCS+LGSSSGTCNSSSTECVL+PLTVVPGKTY
Sbjct: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300
           RLRVSSLTSLSALSFQIEGH+MTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHSMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300

Query: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ 360
           NYWITTNVVSRSRTTPPGLAIFNYYPNHQS+ PPTTPPAPPAWNDV PRLAQSQAIKARQ
Sbjct: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSKRPPTTPPAPPAWNDVPPRLAQSQAIKARQ 360

Query: 361 GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420
           GFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP
Sbjct: 361 GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420

Query: 421 PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480
           PTGYDFKNYDIFNPAPNPEATVSDAI+RLDFNSTVD+ILQNANMMSPNNSETHPWHLHGH
Sbjct: 421 PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDLILQNANMMSPNNSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFD+Y DPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP
Sbjct: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

BLAST of Csa6G500640 vs. NCBI nr
Match: gi|566179811|ref|XP_006380435.1| (L-ascorbate oxidase family protein [Populus trichocarpa])

HSP 1 Score: 899.0 bits (2322), Expect = 4.2e-258
Identity = 421/565 (74.51%), Postives = 475/565 (84.07%), Query Frame = 1

Query: 14  LLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDTVI 73
           L  FV  L+    A++R  +YKWE+K E KSPDC+KK+ +TINGR+PGPTI A Q DTVI
Sbjct: 6   LCFFVISLINIPIADARIRHYKWELKYEYKSPDCYKKLVITINGRTPGPTILAKQNDTVI 65

Query: 74  VELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHA 133
           VE+KNSL TEN AIHWHGIRQIGTPW DGTEGVTQCPILPG+TF Y+FVVDRPGTYLYHA
Sbjct: 66  VEVKNSLLTENTAIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFVYKFVVDRPGTYLYHA 125

Query: 134 HYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGFNW 193
           HYGMQRE G+YGSI V +P+G++EPF+YD DR++ILTDWYHKS++E A GL++    F W
Sbjct: 126 HYGMQREAGIYGSIRVALPDGESEPFAYDYDRSIILTDWYHKSTYEQAAGLSS--IPFQW 185

Query: 194 VGEPDSLLIQGRGRFNCSILGS--SSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLS 253
           VGEP SLLIQG+GRF+CS       +  CN+++ EC L+  TVVPGKTYRLR+SSL++LS
Sbjct: 186 VGEPQSLLIQGKGRFDCSAANPPLKADVCNNTNPECSLYSTTVVPGKTYRLRISSLSALS 245

Query: 254 ALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSR 313
           ALSFQIEGH MTVVEADGHYVEPF V NLFIYSGETYSVL+K DQDPSRNYW TTNVVSR
Sbjct: 246 ALSFQIEGHNMTVVEADGHYVEPFVVKNLFIYSGETYSVLVKTDQDPSRNYWATTNVVSR 305

Query: 314 SRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAAD 373
           + TTPPGLAIFNYYPNH  R PPT PP+ P WND+ PR  QS AIKAR+G I+ PP  +D
Sbjct: 306 NATTPPGLAIFNYYPNHPRRSPPTIPPSGPMWNDIAPRFNQSVAIKARRGHIYPPPATSD 365

Query: 374 KVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDI 433
           +VIVLLNTQNTVNG  RWS+N VSFN+PHTPYLIALK NLLHTFSQ  PP GYDFKNYDI
Sbjct: 366 RVIVLLNTQNTVNGNVRWSVNKVSFNIPHTPYLIALKENLLHTFSQTPPPEGYDFKNYDI 425

Query: 434 FNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGK 493
           F    N  AT SDAIYRL  NSTVDIILQNAN M+PNNSETHPWHLHGHDFWVLGYG+GK
Sbjct: 426 FARQNNTNATTSDAIYRLQLNSTVDIILQNANTMNPNNSETHPWHLHGHDFWVLGYGKGK 485

Query: 494 FDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFE 553
           FD   DP  YNL +PIMKNTVP+HP+GWTALRF ADNPGVWAFHCHI++HFFMGMGVVFE
Sbjct: 486 FDPINDPKNYNLVDPIMKNTVPVHPFGWTALRFKADNPGVWAFHCHIESHFFMGMGVVFE 545

Query: 554 EGIERVGKLPTSIMGCGESKRFLRP 577
           EGIERVGKLP+SIMGCGE+KR L+P
Sbjct: 546 EGIERVGKLPSSIMGCGETKRLLKP 568

BLAST of Csa6G500640 vs. NCBI nr
Match: gi|743923090|ref|XP_011005626.1| (PREDICTED: L-ascorbate oxidase-like [Populus euphratica])

HSP 1 Score: 897.9 bits (2319), Expect = 9.3e-258
Identity = 420/567 (74.07%), Postives = 476/567 (83.95%), Query Frame = 1

Query: 12  LILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDT 71
           L L  FV  L+    AE+R  +YKWE+K E KSPDC+KK+ +TINGR+PGPTI A Q DT
Sbjct: 18  LALCFFVASLINIPIAEARIRHYKWELKYEYKSPDCYKKLVITINGRTPGPTILAKQNDT 77

Query: 72  VIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLY 131
           VIVE+KNSL TEN AIHWHGIRQIGTPW DGTEGVTQCPILPG+TF Y+FVVDRPGTYLY
Sbjct: 78  VIVEVKNSLLTENTAIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFVYKFVVDRPGTYLY 137

Query: 132 HAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGF 191
           HAHYGMQRE G+YGSI V +P+G++EPF+YD DR++ILTDWYHKS++EHA GL++    F
Sbjct: 138 HAHYGMQREAGIYGSIRVALPDGESEPFAYDYDRSIILTDWYHKSTYEHAAGLSS--IPF 197

Query: 192 NWVGEPDSLLIQGRGRFNCSILGS--SSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTS 251
            WVGEP SLLIQG+GRF+C+       +  CN+++ EC L+  TVVPGKTYRLR+SSL++
Sbjct: 198 QWVGEPQSLLIQGKGRFDCAAANPPLKADVCNNTNPECSLYSTTVVPGKTYRLRISSLSA 257

Query: 252 LSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVV 311
           LSALSFQIEGH+MTVVEADGHYVEPF V NLFIYSGETYSVL+K DQDPSRNYW  TNVV
Sbjct: 258 LSALSFQIEGHSMTVVEADGHYVEPFVVKNLFIYSGETYSVLVKTDQDPSRNYWAATNVV 317

Query: 312 SRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKA 371
           SR+ TTPPGLAIFNYYPNH  R PPT PP+ P WND+ PR  QS AIKAR+G I+ PP  
Sbjct: 318 SRNATTPPGLAIFNYYPNHPRRSPPTIPPSGPMWNDIEPRFNQSVAIKARKGHIYYPPAT 377

Query: 372 ADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNY 431
           +D+VIVLLNTQN VNG  RWS+NNVSFN+PHTPYLIALK NLLHTFSQ  PP GYDFKNY
Sbjct: 378 SDRVIVLLNTQNRVNGNVRWSVNNVSFNIPHTPYLIALKENLLHTFSQTPPPEGYDFKNY 437

Query: 432 DIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGE 491
           D+F    N  AT SDAIYRL  NSTVDIILQNAN M+PNNSETHPWHLHGHDFWVLGYG+
Sbjct: 438 DVFARQNNTNATTSDAIYRLQLNSTVDIILQNANTMNPNNSETHPWHLHGHDFWVLGYGK 497

Query: 492 GKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVV 551
           GKFD   DP  YNL +PIMKNTVP+HP+GWTALRF ADNPGVWAFHCHI++HFFMGMGVV
Sbjct: 498 GKFDPINDPKNYNLVDPIMKNTVPVHPFGWTALRFKADNPGVWAFHCHIESHFFMGMGVV 557

Query: 552 FEEGIERVGKLPTSIMGCGESKRFLRP 577
           FEEGIERVGKLP+SIMGCGE+K  L+P
Sbjct: 558 FEEGIERVGKLPSSIMGCGETKHLLKP 582

BLAST of Csa6G500640 vs. NCBI nr
Match: gi|566179815|ref|XP_002309992.2| (hypothetical protein POPTR_0007s05910g [Populus trichocarpa])

HSP 1 Score: 897.1 bits (2317), Expect = 1.6e-257
Identity = 421/567 (74.25%), Postives = 476/567 (83.95%), Query Frame = 1

Query: 12  LILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSPGPTIYAHQGDT 71
           L L  FV  L+    AE+R  +YK+E+K E KSPDC+KK+ +TINGR+PGPTI+A Q DT
Sbjct: 18  LALCFFVISLINIPIAEARIRHYKFELKYEYKSPDCYKKLVITINGRTPGPTIFAQQNDT 77

Query: 72  VIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLY 131
           VIVE+KNSL TEN AIHWHGIRQIGTPW DGTEGVTQCPILPG+TF Y+FVVDRPGTYLY
Sbjct: 78  VIVEVKNSLLTENTAIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFVYKFVVDRPGTYLY 137

Query: 132 HAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEHATGLATPGSGF 191
           HAHYGMQRE G+YGSI V +P+G++EPF+YD DR++ILTDWYHKS++E ATGL++    F
Sbjct: 138 HAHYGMQREAGIYGSIRVALPDGESEPFAYDYDRSIILTDWYHKSTYEQATGLSS--IPF 197

Query: 192 NWVGEPDSLLIQGRGRFNCSILGS--SSGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTS 251
            WVGEP SLLIQG+GRF+CS       +  CN++S EC L+  TVVPGKTYRLR+SSLT+
Sbjct: 198 QWVGEPQSLLIQGKGRFDCSAANPPLKADVCNNTSPECSLYSTTVVPGKTYRLRISSLTA 257

Query: 252 LSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVV 311
           LSALSFQIEGH++TVVEADGHYVEPF V NLFIYSGETYSVL+K DQDPSRNYW TTNVV
Sbjct: 258 LSALSFQIEGHSLTVVEADGHYVEPFVVKNLFIYSGETYSVLVKTDQDPSRNYWATTNVV 317

Query: 312 SRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKA 371
           SR+ TTPPGLAIFNYYPNH  R PPT PP+ P WND+ PR  QS AIKAR+G I++PP  
Sbjct: 318 SRNATTPPGLAIFNYYPNHPRRSPPTIPPSGPMWNDIEPRFNQSVAIKARKGHIYSPPAT 377

Query: 372 ADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNY 431
           +D+VIV LNTQN VNG  RWS+NNVSFN+PHTPYLIALK NLLHTFSQ  PP GYDFKNY
Sbjct: 378 SDRVIVFLNTQNRVNGNVRWSVNNVSFNIPHTPYLIALKENLLHTFSQTPPPEGYDFKNY 437

Query: 432 DIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGE 491
           DIF    N  AT SDAIYRL  NSTVDIILQNAN M+PNNSETHPWHLHGHDFWVLGYG+
Sbjct: 438 DIFARQNNTNATTSDAIYRLQLNSTVDIILQNANTMNPNNSETHPWHLHGHDFWVLGYGK 497

Query: 492 GKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVV 551
           GKFD   DP  YNL +PIMKNTVP+HP GWTALRF ADNPG WAFHCHI++HFFMGMGVV
Sbjct: 498 GKFDPINDPKNYNLVDPIMKNTVPVHPLGWTALRFKADNPGAWAFHCHIESHFFMGMGVV 557

Query: 552 FEEGIERVGKLPTSIMGCGESKRFLRP 577
           FEEGIERVGKLP+SIMGCGE+K  L+P
Sbjct: 558 FEEGIERVGKLPSSIMGCGETKHLLKP 582

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ASO_CUCSA3.7e-17451.68L-ascorbate oxidase OS=Cucumis sativus PE=1 SV=1[more]
ASO_CUCMA7.8e-17251.23L-ascorbate oxidase OS=Cucurbita maxima GN=AAO PE=1 SV=2[more]
ASO_CUCPM3.9e-17151.64L-ascorbate oxidase OS=Cucurbita pepo var. melopepo PE=1 SV=1[more]
ASO_TOBAC1.7e-16650.52L-ascorbate oxidase OS=Nicotiana tabacum GN=AAO PE=2 SV=1[more]
LAC22_ORYSJ7.3e-7733.27Laccase-22 OS=Oryza sativa subsp. japonica GN=LAC22 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KK62_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_6G500640 PE=3 SV=1[more]
U5G9V4_POPTR2.9e-25874.51L-ascorbate oxidase family protein OS=Populus trichocarpa GN=POPTR_0007s05890g P... [more]
B9HFP2_POPTR1.1e-25774.25Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0007s05910g PE=3 SV=2[more]
A0A061DIV4_THECC1.0e-25571.85Cupredoxin superfamily protein isoform 1 OS=Theobroma cacao GN=TCM_001179 PE=3 S... [more]
K4BSL7_SOLLC2.2e-25372.57Uncharacterized protein OS=Solanum lycopersicum PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G39830.18.4e-23367.14 Cupredoxin superfamily protein[more]
AT5G21105.16.1e-16749.73 Plant L-ascorbate oxidase[more]
AT5G21100.12.5e-16049.01 Plant L-ascorbate oxidase[more]
AT5G09360.17.2e-7533.81 laccase 14[more]
AT2G40370.11.0e-7332.07 laccase 5[more]
Match NameE-valueIdentityDescription
gi|449451465|ref|XP_004143482.1|0.0e+00100.00PREDICTED: L-ascorbate oxidase [Cucumis sativus][more]
gi|659080148|ref|XP_008440636.1|0.0e+0095.31PREDICTED: L-ascorbate oxidase [Cucumis melo][more]
gi|566179811|ref|XP_006380435.1|4.2e-25874.51L-ascorbate oxidase family protein [Populus trichocarpa][more]
gi|743923090|ref|XP_011005626.1|9.3e-25874.07PREDICTED: L-ascorbate oxidase-like [Populus euphratica][more]
gi|566179815|ref|XP_002309992.2|1.6e-25774.25hypothetical protein POPTR_0007s05910g [Populus trichocarpa][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001117Cu-oxidase
IPR002355Cu_oxidase_Cu_BS
IPR008972Cupredoxin
IPR011706Cu-oxidase_2
IPR011707Cu-oxidase_3
IPR017760L-ascorbate_oxidase_pln
IPR001117Cu-oxidase
IPR002355Cu_oxidase_Cu_BS
IPR008972Cupredoxin
IPR011706Cu-oxidase_2
IPR017760L-ascorbate_oxidase_pln
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
GO:0005507copper ion binding
GO:0016491oxidoreductase activity
GO:0005507copper ion binding
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO:0055114oxidation-reduction process
Vocabulary: Cellular Component
TermDefinition
GO:0005576extracellular region
GO:0005576extracellular region
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019852 L-ascorbic acid metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006457 protein folding
cellular_component GO:0005576 extracellular region
molecular_function GO:0005507 copper ion binding
molecular_function GO:0008447 L-ascorbate oxidase activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016722 oxidoreductase activity, oxidizing metal ions
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU085621cucumber EST collection version 3.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa6G500640.1Csa6G500640.1mRNA
Csa6G500640.2Csa6G500640.2mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU085621CU085621transcribed_cluster


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 31..190
score: 1.8
IPR002355Multicopper oxidase, copper-binding sitePROSITEPS00080MULTICOPPER_OXIDASE2coord: 399..410
scor
IPR008972CupredoxinGENE3DG3DSA:2.60.40.420coord: 230..421
score: 4.3E-44coord: 24..218
score: 4.6
IPR008972CupredoxinunknownSSF49503Cupredoxinscoord: 231..433
score: 4.49E-47coord: 22..212
score: 2.13
IPR011706Multicopper oxidase, type 2PFAMPF07731Cu-oxidase_2coord: 294..417
score: 1.6
IPR017760L-ascorbate oxidase, plantsTIGRFAMsTIGR03388TIGR03388coord: 1..433
score: 2.2E
NoneNo IPR availablePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 1..440
score: 1.1E
NoneNo IPR availablePANTHERPTHR11709:SF28SUBFAMILY NOT NAMEDcoord: 1..440
score: 1.1E