Cucsa.103690 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.103690
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	tRNA-dihydrouridine synthase
Location	scaffold00927 : 2435884 .. 2440409 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GATGGAGTCCAAATTTCCTAAACCTAAGTCAGAGTTTCGTCCTTAGTTTTCCGGCCTAAAGTTCACTAGAAGAGAAGACGAATAAATAGTTGCCGATTTTCTAGTCCGACTGTCTCCGTTTAAGATGGATTACGAGAACAAATTCGTCCTAGCTCCCATGGTTCGCGTTGTAAGTTAGACCAAATGATAATATCTATTGCTTGAGTTGCTCTCATTACTTGATCGAGTTTATCTTCTAATCTTAGTTTTTGTAAAAAAAaTTGCCGCTCGATTGTCTACATCTAGATGCCAAACAATTATTTGGAATAGTTTGAAGCTAGATTAGTAAATCACTTAATCGTAGTTATTTCTTTCAAAATACCCGCCAGATTGTATCCGTTTAGGTTGCTGTTATTGGAGAATTGTGAATTTGATGAGATTATGTGTTTCGAATTACAAAAGTTTTTTTCATGGAGCTTTTCGTTATTGGCCTTTCATGTTTTCAGGGAACGTTACCGTTTAGATTATTAGCTTCACAACACGGTGCAGACATCACCTATGGCGAGGAAATTATTGACCATAAGATGATCAAATGCGAGCGTAGATTAAATGGTAGTGGTACTTTTCTTCTCTGTATTGTTAAAAAATTGTAGTTATTGATTTTCAATTGTACTCTCACACCAAATGCGATGTTTATGATATAGCAGTGTTACTCTATTCATATTAAGTCTATTTCACCCAGAAAAATGATATATTGACTTCACCTGTAGAGATAAATTGTTGCTGTCGTATGCATATTGGCAATAAGTTAATGTGTTCATTTATTGATAATTATGGATATGCTTGTTTAATGTTATCCTCTATCCATTTTTTtCAATCTTGTATTTGAAGTTATCAATTTGTTGGAATTTTTATTTTTGTCTATAATTAGAAGGAAACATGTGATTGGTTAGCGGCTTAGGAGATGAAGGTTGCATCAAGCATATTTTCTTCCCTTTTCTTCACCAATTATGGTCTAGTGAACTTCCTGTACTTCTTTTCATCCAGAGCATGTAGGTTCAACTGATTTTGTTGAGAAGGGGACAAACAATGTAGTTTTCAGGACCTGCAGCCTTGAAAGAAAACATGTAGTTTTTCAAATGGGTACTTCTAATGCTGCGAGGGCCCTAAGTGCTGCTCAACTAGTGTAAGTTTGTTACTTTAATTTTTTTCCCTTCTTACACAATTGCGGTTCTATAAATTTTGGTGGTGGTTATCGTGACTTCATACTTTGTGCTCTGTACCATATTCTTACTATTATTATTTATCCTTCAATTTATTGTAATAAGCTCTGTTATGGATTGAACTTAATCTTTGTTTATTTACTTGTTCGATTAATGTGATGATGTGATTTATTTCTTAGTAGCCTCTTTTGTGGTCACTTTTTAGTGGCAGTTTTTCTTCCATTATTTAATTTAATGTGATCATCTTGATAACCATACGGGGTTCATTTGGCTTAGTTGATTATGTCCCATACTGTTTCGTTGTGGTTGTGATTAGGTACGAAGAGGAAAAAAGAAGTGAAAATCTTCTGTCAATTTCATGCAAAATGAAACCTATAATTGATTAGTCGTACGTTGACGATGAGCAACAGTATGGAGTCAAGTAGTTGTTATGATGACAGATAAAAGGATTTTTTtCTTGATACCTTTAAATGTTCACTAAGTAAGTGGAAGTTGATTGCTGTGAGCAGTTGCATCAAATCATTAGGCTCTCTATGGATGACTTCTGTGTTAGGGGATAATTGGGTACTTAATTGGTGAAGAATGCTTCGTTTTCTAATGAAACCATATTTGTATAACTGATGGAAAAGTTTCTTTTTGTAGGTGTCAAGACGTTGCTGCCATAGATATCAACATGGGTTGCCCTAAGTCATTTTCCATCAGTGGGGGCATGGGTGCGGCACTATTGAATAAACCAGACCTTATTCATGATGTATGCAATTCATATCCTTACGGATTGATATAATTTCTGATTTATTCTCCCTCTTTTGTACTTCTGGCAATATTACTTTCCTACTCACCTTTTATTTACTTGTTTTTTCAGATTTTGACAACATTGAGGAGAAACTTGAACATTCTAATTACCTGTAAGATTCGACTGTTAAATTCACCTCAGGATACAGTGGAGTTAGCTAGACGAATAGAGAGTACTGGTGTTTCAGCTCTTGGTGTTCATGGCAGGTGATCGTCAAGTTTTTGCAGTTTCCTTCTATTTAAGACACTTATCATTTATGCCCGAAGAATTTAGCTGTAGAATAGGGTTTGTGGTTTGTCTCTAAGCAAGTTGGTTCAAACACTCATGGATATTAAAGAAACGAGTTTGGTTTTTTTtCTTCCCCCCCAAGCAGAGGGTTGAGGAAACACGCTCTCACAAAACTAATAAAGGAGAGTCTGCCAATTGTTAATAGTCAATTACAACAGAGAACATTCATTTGCTACAAAACATTTAGATGTACTTTTGTGCTTTAAGAAGGGCAGTAACTTAGATGTCTCTTGCATTTGAGGCTTTAAAATTTGTAATTCATTCTAGTTCTAAAAGAAATTAGGAATGAGAGATTGCTAGTAAGGAAGCCGAATAATGAATATGTACTACCAAACATGCTCATAGGGAAAAATTTAAAACTATGGTGAATAGTATGCTTTGTTGTTTCTTTCATTTTTTCCCTCTACAATAATGAAAAAAGGAAAAAGAATTGAGCTGCATTGCCTCAGCTGAAAACAAAAGAAAGTGAAAGAATAAACTAGATTTGATCAAACCTTTTAGTTCCAAATATACTTCTGTTAGCTTTGCATGAATGCTTTGTTTTCATTCTATTTTAATTGGTTTTTGTAGTCATGCTCGATAACTTGCAGAAGAGTTGCAGATAGACCAAGAGATCCAGCTAAATGGAGTGAGATTGCAGATGTTGTAGCAGCATTGACTATTCCTGTTATTGCAAATGGTGACATCTTTGAGTATGATGATTTTCAACGGATCAAAGTTCATACAGGTATGTATGTACAGAAGGCACTGTTTTTGTTTtGTTTtGGGGTGTGTGCGTGGGAGGAGGGGTGGTACTACTAATTAGAACTCTTTAAAACTGATTGTACCACATTCGTTAAGAGGTTGCTTTTACCTGGCTTGACCTCTTTCATTTCCTTTACTGGTCCACTCGTAAATAAAACAGATAACGGCCATATTTACTAGAAACACTCATTTAGCTTTTGATATACCAACCAGTGAAGCAATATGGTGATAGCGAGTTAGTTTTGAACTAGAGGTAGAGGTCCAAAATGTCTTATAAGTTTCCTGGCTACCGTCATGTACTGTCAGAAAATAGTTTTGCATGGATAATTAAGCCATGCACAAGCCACAAGCACAACATTTCCAACCATCGATTGTAAAAATCCAGTTATCCAATAATTGCCGAGTGTGGAAGAAACAATTTGGAAAAAATTTCAGTTCTTAACCTACAAGGCTGAAATAGTTCCAATGCTTTTTGAATTAGCAAATAAACTAATTACGGTGTTACTGTTACTTCAACTTGTTAACCATGTGGAATGGTGTAAACAAATGGTCTTGATACTTTGCGTTCCTCTGGTGGAAGAGAGTGTGTGGTTAAATTTAATTGACATGAATGAACTATAGTAAAGTTGTGATTCATCAGGATGAAGCTGCTTGGGCCGACAGATGCTATTTCAAGCAGTTTAGGTTTTtACTTGTCTGTCCATTCTCTCTTGGTTTAAATATTAAAACAATCTGGTATTGCGCAAGGTGCTTCTTCAATGATGGTTGCTCGAGGAGCCCTTTGGAATGCTTCAATCTTCTCTCCAGAAGGCAAATTACCATGGGAAGATGTGAAAAGAGAATATGTTAGGAAGGTGAGGTACTTATCATGCACACAGTTCATTCAATCAATTAATTAGTTAATCAGAGTGAATGCTTAGAGCTTTACAGTGCCCTCACTAGATTTTACGTATGTAGATACACACACAAACAGAATTGAAGTGATTAAATTCTTGTGTTGAACTCAGAGCATCTTGTGGGATAATGATATCAAAAGTACGAAGTACACGCTAAAAGAAATGATAATGCATCATTCTTGTCTTGAACTGCCAGAAGGAAAGGCCGTGATAAAATCTGAATCCCTTGCAGATCTAGCGTAAGTAAATGCTATTAGTTTACAACCACTATTGCCATTTTAACTTTTGAGCTAGCTTTGAGCTGATTCGCCATTAAACATTCCATCTTTTGTTTtCTTTATGATATATAGGAAACTTTATGGTGAGGAAAAATACTATCAATTCGTTCAAGAGAATCGGTCTTTCAATGAAAGTAGATGATGGCGTTTCACAGAGATGCTTGTTGCCATACTTTCAACAGAGAAACCTAACAGACTAGGAGAACCTTGCAGCTACAATGTCGTTTTATTCTCAGTAAAACCCATTTAATTGATTATACAGATGCATCAGGTATATTACCGAATCATTCTTTGGCATAGTATGGAGAAGTTGAATGCTTAAGGC

mRNA sequence

GATGGAGTCCAAATTTCCTAAACCTAAGTCAGAGTTTCGTCCTTAGTTTTCCGGCCTAAAGTTCACTAGAAGAGAAGACGAATAAATAGTTGCCGATTTTCTAGTCCGACTGTCTCCGTTTAAGATGGATTACGAGAACAAATTCGTCCTAGCTCCCATGGTTCGCGTTGGAACGTTACCGTTTAGATTATTAGCTTCACAACACGGTGCAGACATCACCTATGGCGAGGAAATTATTGACCATAAGATGATCAAATGCGAGCGTAGATTAAATGAGCATGTAGGTTCAACTGATTTTGTTGAGAAGGGGACAAACAATGTAGTTTTCAGGACCTGCAGCCTTGAAAGAAAACATGTAGTTTTTCAAATGGGTACTTCTAATGCTGCGAGGGCCCTAAGTGCTGCTCAACTAGTGTGTCAAGACGTTGCTGCCATAGATATCAACATGGGTTGCCCTAAGTCATTTTCCATCAGTGGGGGCATGGGTGCGGCACTATTGAATAAACCAGACCTTATTCATGATATTTTGACAACATTGAGGAGAAACTTGAACATTCTAATTACCTGTAAGATTCGACTGTTAAATTCACCTCAGGATACAGTGGAGTTAGCTAGACGAATAGAGAGTACTGGTGTTTCAGCTCTTGGTGTTCATGGCAGAAGAGTTGCAGATAGACCAAGAGATCCAGCTAAATGGAGTGAGATTGCAGATGTTGTAGCAGCATTGACTATTCCTGTTATTGCAAATGGTGACATCTTTGAGTATGATGATTTTCAACGGATCAAAGTTCATACAGGTGCTTCTTCAATGATGGTTGCTCGAGGAGCCCTTTGGAATGCTTCAATCTTCTCTCCAGAAGGCAAATTACCATGGGAAGATGTGAAAAGAGAATATGTTAGGAAGAGCATCTTGTGGGATAATGATATCAAAAGTACGAAGTACACGCTAAAAGAAATGATAATGCATCATTCTTGTCTTGAACTGCCAGAAGGAAAGGCCGTGATAAAATCTGAATCCCTTGCAGATCTAGCGAAACTTTATGGTGAGGAAAAATACTATCAATTCGTTCAAGAGAATCGGTCTTTCAATGAAAGTAGATGATGGCGTTTCACAGAGATGCTTGTTGCCATACTTTCAACAGAGAAACCTAACAGACTAGGAGAACCTTGCAGCTACAATGTCGTTTTATTCTCAGTAAAACCCATTTAATTGATTATACAGATGCATCAGGTATATTACCGAATCATTCTTTGGCATAGTATGGAGAAGTTGAATGCTTAAGGC

Coding sequence (CDS)

ATGGATTACGAGAACAAATTCGTCCTAGCTCCCATGGTTCGCGTTGGAACGTTACCGTTTAGATTATTAGCTTCACAACACGGTGCAGACATCACCTATGGCGAGGAAATTATTGACCATAAGATGATCAAATGCGAGCGTAGATTAAATGAGCATGTAGGTTCAACTGATTTTGTTGAGAAGGGGACAAACAATGTAGTTTTCAGGACCTGCAGCCTTGAAAGAAAACATGTAGTTTTTCAAATGGGTACTTCTAATGCTGCGAGGGCCCTAAGTGCTGCTCAACTAGTGTGTCAAGACGTTGCTGCCATAGATATCAACATGGGTTGCCCTAAGTCATTTTCCATCAGTGGGGGCATGGGTGCGGCACTATTGAATAAACCAGACCTTATTCATGATATTTTGACAACATTGAGGAGAAACTTGAACATTCTAATTACCTGTAAGATTCGACTGTTAAATTCACCTCAGGATACAGTGGAGTTAGCTAGACGAATAGAGAGTACTGGTGTTTCAGCTCTTGGTGTTCATGGCAGAAGAGTTGCAGATAGACCAAGAGATCCAGCTAAATGGAGTGAGATTGCAGATGTTGTAGCAGCATTGACTATTCCTGTTATTGCAAATGGTGACATCTTTGAGTATGATGATTTTCAACGGATCAAAGTTCATACAGGTGCTTCTTCAATGATGGTTGCTCGAGGAGCCCTTTGGAATGCTTCAATCTTCTCTCCAGAAGGCAAATTACCATGGGAAGATGTGAAAAGAGAATATGTTAGGAAGAGCATCTTGTGGGATAATGATATCAAAAGTACGAAGTACACGCTAAAAGAAATGATAATGCATCATTCTTGTCTTGAACTGCCAGAAGGAAAGGCCGTGATAAAATCTGAATCCCTTGCAGATCTAGCGAAACTTTATGGTGAGGAAAAATACTATCAATTCGTTCAAGAGAATCGGTCTTTCAATGAAAGTAGATGA

Protein sequence

MDYENKFVLAPMVRVGTLPFRLLASQHGADITYGEEIIDHKMIKCERRLNEHVGSTDFVEKGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGMGAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRRVADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNASIFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLADLAKLYGEEKYYQFVQENRSFNESR*

Homology

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BLAST of Cucsa.103690 vs. Swiss-Prot
Match: DUS2L_HUMAN (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like OS=Homo sapiens GN=DUS2 PE=1 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 3.4e-79
Identity = 157/327 (48.01%), Postives = 217/327 (66.36%), Query Frame = 1


Query: 3   YENKFVLAPMVRVGTLPFRLLASQHGADITYGEEIIDHKMIKCERRLNEHVGSTDFVEKG 62
           Y NK +LAPMVRVGTLP RLLA  +GADI Y EE+ID KMI+C+R +NE + + DFV   
Sbjct: 10  YHNKLILAPMVRVGTLPMRLLALDYGADIVYCEELIDLKMIQCKRVVNEVLSTVDFVAPD 69

Query: 63  TNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGMGA 122
            + VVFRTC  E+  VVFQMGTS+A RAL+ A+LV  DVA ID+NMGCPK +S  GGMGA
Sbjct: 70  -DRVVFRTCEREQNRVVFQMGTSDAERALAVARLVENDVAGIDVNMGCPKQYSTKGGMGA 129

Query: 123 ALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRRVA 182
           ALL+ PD I  IL+TL +     +TCKIR+L S +DT+ L +RIE TG++A+ VHGR+  
Sbjct: 130 ALLSDPDKIEKILSTLVKGTRRPVTCKIRILPSLEDTLSLVKRIERTGIAAIAVHGRKRE 189

Query: 183 DRPRDPAKWSEIADVVAALTIPVIANG----DIFEYDDFQRIKVHTGASSMMVARGALWN 242
           +RP+ P     I  +   L+IPVIANG     I +Y D +  +  T ASS+MVAR A+WN
Sbjct: 190 ERPQHPVSCEVIKAIADTLSIPVIANGGSHDHIQQYSDIEDFRQATAASSVMVARAAMWN 249

Query: 243 ASIFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSES 302
            SIF  EG  P E+V ++Y+R ++ +DN   +TKY L +M+     LE P+G+ +  ++S
Sbjct: 250 PSIFLKEGLRPLEEVMQKYIRYAVQYDNHYTNTKYCLCQMLREQ--LESPQGRLLHAAQS 309

Query: 303 LADLAKLYGEEKYYQFVQENRSFNESR 326
             ++ + +G   +Y+   +     ++R
Sbjct: 310 SREICEAFGLGAFYEETTQELDAQQAR 333

[Close]

BLAST of Cucsa.103690 vs. Swiss-Prot
Match: DUS2L_MOUSE (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like OS=Mus musculus GN=Dus2 PE=1 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.1e-77
Identity = 154/315 (48.89%), Postives = 213/315 (67.62%), Query Frame = 1


Query: 3   YENKFVLAPMVRVGTLPFRLLASQHGADITYGEEIIDHKMIKCERRLNEHVGSTDFVEKG 62
           Y NK +LAPMVRVGTLP RLLA  +GADI Y EE+ID KM++C+R +NE + + DFV   
Sbjct: 10  YHNKLILAPMVRVGTLPMRLLALDYGADIVYCEELIDLKMLQCKRVVNEVLSTVDFVAPD 69

Query: 63  TNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGMGA 122
            + VVFRTC  E+  VVFQMGTS+A RAL+ A+LV  DVA ID+NMGCPK +S  GGMGA
Sbjct: 70  -DRVVFRTCEREQSRVVFQMGTSDAERALAVARLVENDVAGIDVNMGCPKEYSTKGGMGA 129

Query: 123 ALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRRVA 182
           ALL+ PD I  IL+TL +  +  +TCKIR+L S +DT+ L +RIE TG+SA+ VHGR   
Sbjct: 130 ALLSDPDKIEKILSTLVKGTHRPVTCKIRILPSLEDTLNLVKRIERTGISAIAVHGRNRD 189

Query: 183 DRPRDPAKWSEIADVVAALTIPVIANG----DIFEYDDFQRIKVHTGASSMMVARGALWN 242
           +RP+ P     I  +   L+IPVIANG     I ++ D +  +  T ASS+MVAR A+WN
Sbjct: 190 ERPQHPVSCEVIRAIAETLSIPVIANGGSHDHIQQHVDIEDFRQATAASSVMVARAAMWN 249

Query: 243 ASIFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSES 302
            SIF  +G  P E+V ++Y+R ++ +DN   +TKY L +M+     LE P+G+ +  ++S
Sbjct: 250 PSIFLKDGLRPLEEVMQKYIRYAVQYDNHYTNTKYCLCQMLREQ--LESPQGRLLHAAQS 309

Query: 303 LADLAKLYGEEKYYQ 314
             ++ + +G   +Y+
Sbjct: 310 SQEICEAFGLGAFYE 321

[Close]

BLAST of Cucsa.103690 vs. Swiss-Prot
Match: DUS2_SCHPO (tRNA-dihydrouridine(20) synthase [NAD(P)+] OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=dus2 PE=3 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.7e-51
Identity = 119/320 (37.19%), Postives = 180/320 (56.25%), Query Frame = 1


Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQHGADITYGEEIIDHKMIK---CERRLNEHVGSTD 60
           ++Y NK  LAPMVR+G LP RLLA ++GA++ +G EI+D  ++     ER +N+ +   D
Sbjct: 4   LNYSNKVCLAPMVRIGELPMRLLALRYGANLVWGPEIVDKALLSGTPVERVVNDRINCID 63

Query: 61  FVEKGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSIS 120
           FV+  +N V+FR   LE   ++FQ+G+++   A+ AA+LV  DVA ID+N GCPK FS+ 
Sbjct: 64  FVKPPSNKVLFRVHPLEANRLIFQLGSASPELAVEAAKLVANDVAGIDLNCGCPKHFSVH 123

Query: 121 GGMGAALLNKPDLIHDILTTLRRNL----NILITCKIRLLNSPQDTVELARRIESTGVSA 180
            GMGA LL   D +  IL  L   +     I I+CKIRLL + +DT++L  RI  TGV A
Sbjct: 124 AGMGAGLLKNQDRLVSILDALVNEIGKPYKISISCKIRLLETKEDTLKLVERICDTGVRA 183

Query: 181 LGVHGRRVADRPRDPAKWSEIADVVAAL---TIPVIANGDIFEYDDFQRIKVHTGASSMM 240
           + VH R    R  +PA  S ++++V       + ++ NGD+  Y+D   +    G   ++
Sbjct: 184 ITVHCRTTPMRNTEPADRSYLSEIVGVCRNKDVSILVNGDVLSYNDGLDVIEKYGVDGVL 243

Query: 241 VARGALWNASIFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEG 300
           +AR A  N S F  EG L    V  E+++ ++  DN+  +TKY L +++         +G
Sbjct: 244 IARAAERNVSCFRIEGPLSSFKVAEEFLKMALEVDNNFGNTKYCLNQIM---------QG 303

Query: 301 KAVIKSESLADLAKLYGEEK 311
                   LA  AK Y + K
Sbjct: 304 SFRKNVRQLAQTAKTYEDLK 314

[Close]

BLAST of Cucsa.103690 vs. Swiss-Prot
Match: DUS2_YEAST (tRNA-dihydrouridine(20) synthase [NAD(P)+] OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SMM1 PE=1 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 3.6e-41
Identity = 111/298 (37.25%), Postives = 169/298 (56.71%), Query Frame = 1


Query: 3   YENKFVLAPMVRVGTLPFRLLASQHGADITYGEEIIDHKMIKCERRLNEHVGSTDFV--- 62
           Y  K VLAPMVR G LP RL+A  HGAD+ +  EIID K+I+C R+ N  + + D+V   
Sbjct: 4   YAGKLVLAPMVRAGELPTRLMALAHGADLVWSPEIIDKKLIQCVRKENTALQTVDYVVPS 63

Query: 63  --EKGTNNVVFRTC-SLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSI 122
             +     +VFRT   LE   ++FQ+G+++ A A  AA  V  DV+ IDIN GCPK FSI
Sbjct: 64  KVQTRPETLVFRTYPKLESSKLIFQIGSASPALATQAALKVINDVSGIDINAGCPKHFSI 123

Query: 123 SGGMGAALLNKPDLIHDILTTLRRNL----NILITCKIRLLNSPQDTVELARRIESTGVS 182
             GMG+ALL  PD +  IL  L +N+    +  I+ KIRLL++ QDT++L +R+ +TG++
Sbjct: 124 HSGMGSALLRTPDTLCLILKELVKNVGNPHSKPISVKIRLLDTKQDTLQLVKRLCATGIT 183

Query: 183 ALGVHGRRVADRPRDPA---KWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSM 242
            L VH R+   R R+       +EI ++  A  + +I NG I +   F  ++ +   ++ 
Sbjct: 184 NLTVHCRKTEMRNREQPITDYIAEIYEICQANNVSLIVNGAIRDRSHFHDLQANHWKNTN 243

Query: 243 ---MVARGALWNASIFSPEGK-----LPWEDVKREYVRKSILWDNDIKSTKYTLKEMI 280
              M+A  A  + ++F    K       W    RE+++ +  +DN I +TKY L  ++
Sbjct: 244 IGGMIAECAERDPTVFDHTSKPSEDGPSWVVACREFIQWATKFDNHIGNTKYMLSRIV 301

[Close]

BLAST of Cucsa.103690 vs. Swiss-Prot
Match: DUS4L_MOUSE (tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like OS=Mus musculus GN=Dus4l PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.6e-28
Identity = 84/258 (32.56%), Postives = 131/258 (50.78%), Query Frame = 1


Query: 8   VLAPMVRVGTLPFRLLASQHGADITYGEEIIDHKMIKCERRLNEHVGSTDFVEKGTNNVV 67
           V APMVR   L FR L  ++  D+ Y   II    ++  +  +         E  TN   
Sbjct: 30  VCAPMVRYSKLAFRTLVRKYSCDLCYTPMIIAADFVRSIKARDS--------EFTTNQ-- 89

Query: 68  FRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGMGAALLNK 127
              C L     + Q   ++A     AA LVC     IDIN GCP+ ++++ G GA L+NK
Sbjct: 90  -GDCPL-----IVQFAANDARLLSDAALLVCPYANGIDINCGCPQRWAMADGYGACLINK 149

Query: 128 PDLIHDILTTLRRNL---NILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRRVADR 187
           P+L+HD++  +R  +      ++ KIR+ +    T++L R+ E+TGVS + VHGR V +R
Sbjct: 150 PELVHDMVRQVRNRVESPRFSVSIKIRIHDDLARTIDLCRKAEATGVSWITVHGRTVEER 209

Query: 188 PRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNASIFSP 247
              P  +  I  +   ++IP++ANGDI    + + +   TG   +MVARG L N ++F+ 
Sbjct: 210 -HQPVHYDAIKMIKENVSIPIVANGDIRSLKEAENVWQMTGTDGVMVARGLLANPAMFAG 269

Query: 248 EGKLP------WEDVKRE 257
             + P      W D+  E
Sbjct: 270 YEETPLKCIWDWVDISLE 270

[Close]

BLAST of Cucsa.103690 vs. TrEMBL
Match: A0A0A0KGL3_CUCSA (tRNA-dihydrouridine synthase OS=Cucumis sativus GN=Csa_6G497190 PE=3 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 6.6e-183
Identity = 324/325 (99.69%), Postives = 325/325 (100.00%), Query Frame = 1


Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQHGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDYENKFVLAPMVRVGTLPFRLLASQ+GADITYGEEIIDHKMIKCERRLNEHVGSTDFVE
Sbjct: 1   MDYENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR
Sbjct: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA
Sbjct: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300

Query: 301 DLAKLYGEEKYYQFVQENRSFNESR 326
           DLAKLYGEEKYYQFVQENRSFNESR
Sbjct: 301 DLAKLYGEEKYYQFVQENRSFNESR 325

[Close]

BLAST of Cucsa.103690 vs. TrEMBL
Match: A5ALC4_VITVI (tRNA-dihydrouridine synthase OS=Vitis vinifera GN=VITISV_024917 PE=3 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 1.9e-153
Identity = 267/319 (83.70%), Postives = 299/319 (93.73%), Query Frame = 1


Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQHGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           M+Y N+ VLAPMVRVGTLPFRLLA+Q+GADITYGEEIIDHK+IKCERRLNE +GS DFVE
Sbjct: 1   MEYGNRLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKLIKCERRLNEDIGSIDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGT+NVVFRTC  E+  VVFQMGTSNA RAL+AAQ+VC+DVAA+DINMGCPKSFSISGGM
Sbjct: 61  KGTHNVVFRTCHEEKNRVVFQMGTSNAERALTAAQIVCKDVAAVDINMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALL +P+LIHDILT L+RNL+I +TCKIRLL S Q+TVELA++IE TGV+AL VHGRR
Sbjct: 121 GAALLTRPELIHDILTILKRNLDIPVTCKIRLLKSSQETVELAQQIEKTGVAALAVHGRR 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           +ADRPRDPAKW+EIADVVAAL+IPVIANGD+F YDDFQRIKV TGASS+MVARGALWNAS
Sbjct: 181 IADRPRDPAKWNEIADVVAALSIPVIANGDVFGYDDFQRIKVATGASSVMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           +FSPEGK+PWEDVK EY+RKSILWDNDIKSTK+TLKEMIMHHSCLELPEGKAVIKSE+LA
Sbjct: 241 VFSPEGKVPWEDVKXEYIRKSILWDNDIKSTKHTLKEMIMHHSCLELPEGKAVIKSETLA 300

Query: 301 DLAKLYGEEKYYQFVQENR 320
           DLAKLYGEEKYYQFV+ NR
Sbjct: 301 DLAKLYGEEKYYQFVRGNR 319

[Close]

BLAST of Cucsa.103690 vs. TrEMBL
Match: A0A067KB11_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_18371 PE=4 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 5.5e-153
Identity = 266/319 (83.39%), Postives = 299/319 (93.73%), Query Frame = 1


Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQHGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           M+YENK VLAPMVRVGTLPFRLLA+++GADITYGEEIIDHKM+KCER++NE++G TDFVE
Sbjct: 1   MEYENKLVLAPMVRVGTLPFRLLAAEYGADITYGEEIIDHKMLKCERKVNEYIGCTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGT NVVFRTC+ ER  VVFQMGTS+A RAL+AAQ+VC+DVAAID+NMGCPKSFSISGGM
Sbjct: 61  KGTENVVFRTCNQERNRVVFQMGTSDAVRALTAAQMVCKDVAAIDVNMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALLNKP+LIHDILTTL+RNLN  +TCKIRLL S QDTVELARRIE TGVSAL VHGR+
Sbjct: 121 GAALLNKPELIHDILTTLKRNLNSPVTCKIRLLKSSQDTVELARRIEKTGVSALAVHGRK 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           VADRPRDPAKWSEIADVV+AL+IPVIANGD+F+Y DF+RIK  TGASS+MVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVSALSIPVIANGDVFDYSDFERIKTATGASSVMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSPEGK+ +EDVKREYVRKSILWDN+IKSTK+TLKEMIMH+SCLELPEGK VIKSE+LA
Sbjct: 241 IFSPEGKVHYEDVKREYVRKSILWDNNIKSTKHTLKEMIMHYSCLELPEGKGVIKSETLA 300

Query: 301 DLAKLYGEEKYYQFVQENR 320
           D+AKLYGEE YYQFV +NR
Sbjct: 301 DIAKLYGEENYYQFVNDNR 319

[Close]

BLAST of Cucsa.103690 vs. TrEMBL
Match: V4SKP9_9ROSI (tRNA-dihydrouridine synthase OS=Citrus clementina GN=CICLE_v10026068mg PE=3 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 1.3e-151
Identity = 262/319 (82.13%), Postives = 298/319 (93.42%), Query Frame = 1


Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQHGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDY+NK VLAPMVRVGTLPFRLLA+Q+GADITYGEEIIDHK++KCERR+NE++GSTDFVE
Sbjct: 1   MDYQNKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKLLKCERRVNEYIGSTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGT++VVFRTC  ER HVVFQMGTS+A RAL+AA++VC+DVAAIDINMGCPKSFS+SGGM
Sbjct: 61  KGTDSVVFRTCHQERNHVVFQMGTSDAVRALTAAKMVCKDVAAIDINMGCPKSFSVSGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALL+KP+LIHDILT L+RNL++ +TCKIRLL S QDTVELAR+IE TGVSAL VHGR+
Sbjct: 121 GAALLSKPELIHDILTMLKRNLDVPVTCKIRLLKSSQDTVELARQIEKTGVSALAVHGRK 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           V DRPRDPAKW EIAD+VAAL+IPVIANGD+FEYDDFQRIK  TGASS+M ARGALWNAS
Sbjct: 181 VVDRPRDPAKWGEIADIVAALSIPVIANGDVFEYDDFQRIKTATGASSVMAARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFS +GKL WEDVKREYVRKSILW+ND+KSTK+TLKEMIMH+S LELPEGKA+IKSE+LA
Sbjct: 241 IFSSQGKLHWEDVKREYVRKSILWENDVKSTKHTLKEMIMHYSSLELPEGKAIIKSETLA 300

Query: 301 DLAKLYGEEKYYQFVQENR 320
           D+AKLY EEKYYQFV ENR
Sbjct: 301 DIAKLYEEEKYYQFVNENR 319

[Close]

BLAST of Cucsa.103690 vs. TrEMBL
Match: A0A061DPF2_THECC (tRNA-dihydrouridine synthase OS=Theobroma cacao GN=TCM_000905 PE=3 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 3.9e-151
Identity = 263/319 (82.45%), Postives = 296/319 (92.79%), Query Frame = 1


Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQHGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDY NK VLAPMVRVGTLPFRLLA+ +GADITY EEIIDHK+IKCER++NE++GSTDFVE
Sbjct: 1   MDYGNKLVLAPMVRVGTLPFRLLAADYGADITYAEEIIDHKLIKCERKVNEYIGSTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGT+NVVFRTC+ ER  VVFQMGTS+A RAL AAQ+VC+DVAA+DINMGCPKSFS+SGGM
Sbjct: 61  KGTDNVVFRTCNQERNQVVFQMGTSDAVRALKAAQIVCKDVAAVDINMGCPKSFSVSGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALL KPDLIHDILTTLRRNL+  +TCKIRLL S QDTVELARRIE TGVSAL VHGR+
Sbjct: 121 GAALLTKPDLIHDILTTLRRNLDTPVTCKIRLLKSSQDTVELARRIEKTGVSALAVHGRK 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           VADRPRDPAKW+EIAD+V+AL+IPVIANGD+FEYDDFQRIKV TGASS+MVARGALWNAS
Sbjct: 181 VADRPRDPAKWNEIADIVSALSIPVIANGDVFEYDDFQRIKVATGASSVMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFS +GK  WEDVKREYVRKSILWDND+KSTK+TL+E+I H+SCLELPEGKAVIKSE+LA
Sbjct: 241 IFSSKGKAHWEDVKREYVRKSILWDNDVKSTKHTLREIITHYSCLELPEGKAVIKSENLA 300

Query: 301 DLAKLYGEEKYYQFVQENR 320
           DLAK+YGEE+YY+ V ENR
Sbjct: 301 DLAKVYGEEEYYRLVSENR 319

[Close]

BLAST of Cucsa.103690 vs. TAIR10
Match: AT3G49640.1 (AT3G49640.1 Aldolase-type TIM barrel family protein)

HSP 1 Score: 490.0 bits (1260), Expect = 1.2e-138
Identity = 233/316 (73.73%), Postives = 278/316 (87.97%), Query Frame = 1


Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQHGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDY+NK VLAPMVRVGTL FR+LA+++GADITYGEEIIDHK++KCERRLN   G+++FVE
Sbjct: 1   MDYQNKLVLAPMVRVGTLSFRMLAAEYGADITYGEEIIDHKLVKCERRLNVASGTSEFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGT+NVVF TC  E+  VVFQMGTS+A RAL A+++VC DVA IDINMGCPK+FSI GGM
Sbjct: 61  KGTDNVVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALL+KP+LIHDIL TL+RNL++ +TCKIRLL SP DTVELARRIE  GV AL VHGR+
Sbjct: 121 GAALLSKPELIHDILATLKRNLDVPVTCKIRLLKSPADTVELARRIEKLGVPALAVHGRK 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           +ADRPRDPAKW EIADVVAAL+IPVIANGD+ EYDDF RIK  TGA+S+MVARGA+WNAS
Sbjct: 181 IADRPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATGAASVMVARGAMWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSP+GK  WEDVK++Y+RKSILW+ND+KSTKYT+KEMI HHSCLELPEGK++ K+++L 
Sbjct: 241 IFSPKGKSHWEDVKKKYLRKSILWNNDVKSTKYTIKEMIAHHSCLELPEGKSINKADTLE 300

Query: 301 DLAKLYGEEKYYQFVQ 317
           DLA+LY  E Y+  V+
Sbjct: 301 DLARLYDLEDYFWTVK 316

[Close]

BLAST of Cucsa.103690 vs. TAIR10
Match: AT5G67220.1 (AT5G67220.1 FMN-linked oxidoreductases superfamily protein)

HSP 1 Score: 119.0 bits (297), Expect = 5.4e-27
Identity = 77/247 (31.17%), Postives = 121/247 (48.99%), Query Frame = 1


Query: 6   KFVLAPMVRVGTLPFRLLASQHGADITYGEEIIDHKMIKCERRLNEHVGSTDFVEKGTNN 65
           K+++APMV    LPFRLL  ++GA   Y   +      + E+  N+              
Sbjct: 89  KYIVAPMVDNSELPFRLLCQKYGAQAAYTPMLHSRIFTETEKYRNQE------------- 148

Query: 66  VVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGMGAALL 125
             F TC  +R   V Q   ++    L AA+ V      +DIN+GCP+  +  G  GA L+
Sbjct: 149 --FTTCKEDRPLFV-QFCANDPDTLLEAAKRVEPYCDYVDINLGCPQRIARRGNYGAFLM 208

Query: 126 NKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR--VAD 185
           +   L+  ++  L +NLN+ ++CKIR+  + +DT++ A+ +E  G S L VHGR     D
Sbjct: 209 DNLPLVKSLVEKLAQNLNVPVSCKIRIFPNLEDTLKYAKMLEDAGCSLLAVHGRTRDEKD 268

Query: 186 RPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNASIFS 245
             +  A WS I +V  A+ IPV+ANG++   +D       TG   ++ A   L N + F+
Sbjct: 269 GKKFRADWSAIKEVKNAMRIPVLANGNVRCIEDVDNCIKETGVEGVLSAETLLENPAAFA 319

Query: 246 PEGKLPW 251
                 W
Sbjct: 329 GFRTAEW 319

[Close]

BLAST of Cucsa.103690 vs. NCBI nr
Match: gi|449451357|ref|XP_004143428.1| (PREDICTED: tRNA-dihydrouridine(20) synthase [NAD(P)+])

HSP 1 Score: 647.9 bits (1670), Expect = 9.5e-183
Identity = 324/325 (99.69%), Postives = 325/325 (100.00%), Query Frame = 1


Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQHGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDYENKFVLAPMVRVGTLPFRLLASQ+GADITYGEEIIDHKMIKCERRLNEHVGSTDFVE
Sbjct: 1   MDYENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR
Sbjct: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA
Sbjct: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300

Query: 301 DLAKLYGEEKYYQFVQENRSFNESR 326
           DLAKLYGEEKYYQFVQENRSFNESR
Sbjct: 301 DLAKLYGEEKYYQFVQENRSFNESR 325

[Close]

BLAST of Cucsa.103690 vs. NCBI nr
Match: gi|659079878|ref|XP_008440492.1| (PREDICTED: tRNA-dihydrouridine(20) synthase [NAD(P)+])

HSP 1 Score: 636.7 bits (1641), Expect = 2.2e-179
Identity = 317/325 (97.54%), Postives = 323/325 (99.38%), Query Frame = 1


Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQHGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDYENK VLAPMVRVGTLPFRLLASQ+GADITYGEEIIDHKMIKCERRLNEHVGSTDFVE
Sbjct: 1   MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVC+DVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALLNKPDLIHDILTTLRRNL++LITCKIRLLNSPQDTVELARRIE+TGVSALGVHGRR
Sbjct: 121 GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASS+MVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA
Sbjct: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300

Query: 301 DLAKLYGEEKYYQFVQENRSFNESR 326
           DLAKLYGEEKYYQFVQENRSFN SR
Sbjct: 301 DLAKLYGEEKYYQFVQENRSFNGSR 325

[Close]

BLAST of Cucsa.103690 vs. NCBI nr
Match: gi|147797460|emb|CAN64677.1| (hypothetical protein VITISV_024917 [Vitis vinifera])

HSP 1 Score: 550.1 bits (1416), Expect = 2.7e-153
Identity = 267/319 (83.70%), Postives = 299/319 (93.73%), Query Frame = 1


Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQHGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           M+Y N+ VLAPMVRVGTLPFRLLA+Q+GADITYGEEIIDHK+IKCERRLNE +GS DFVE
Sbjct: 1   MEYGNRLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKLIKCERRLNEDIGSIDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGT+NVVFRTC  E+  VVFQMGTSNA RAL+AAQ+VC+DVAA+DINMGCPKSFSISGGM
Sbjct: 61  KGTHNVVFRTCHEEKNRVVFQMGTSNAERALTAAQIVCKDVAAVDINMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALL +P+LIHDILT L+RNL+I +TCKIRLL S Q+TVELA++IE TGV+AL VHGRR
Sbjct: 121 GAALLTRPELIHDILTILKRNLDIPVTCKIRLLKSSQETVELAQQIEKTGVAALAVHGRR 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           +ADRPRDPAKW+EIADVVAAL+IPVIANGD+F YDDFQRIKV TGASS+MVARGALWNAS
Sbjct: 181 IADRPRDPAKWNEIADVVAALSIPVIANGDVFGYDDFQRIKVATGASSVMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           +FSPEGK+PWEDVK EY+RKSILWDNDIKSTK+TLKEMIMHHSCLELPEGKAVIKSE+LA
Sbjct: 241 VFSPEGKVPWEDVKXEYIRKSILWDNDIKSTKHTLKEMIMHHSCLELPEGKAVIKSETLA 300

Query: 301 DLAKLYGEEKYYQFVQENR 320
           DLAKLYGEEKYYQFV+ NR
Sbjct: 301 DLAKLYGEEKYYQFVRGNR 319

[Close]

BLAST of Cucsa.103690 vs. NCBI nr
Match: gi|802681614|ref|XP_012082133.1| (PREDICTED: tRNA-dihydrouridine(20) synthase [NAD(P)+])

HSP 1 Score: 548.5 bits (1412), Expect = 7.8e-153
Identity = 266/319 (83.39%), Postives = 299/319 (93.73%), Query Frame = 1


Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQHGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           M+YENK VLAPMVRVGTLPFRLLA+++GADITYGEEIIDHKM+KCER++NE++G TDFVE
Sbjct: 1   MEYENKLVLAPMVRVGTLPFRLLAAEYGADITYGEEIIDHKMLKCERKVNEYIGCTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGT NVVFRTC+ ER  VVFQMGTS+A RAL+AAQ+VC+DVAAID+NMGCPKSFSISGGM
Sbjct: 61  KGTENVVFRTCNQERNRVVFQMGTSDAVRALTAAQMVCKDVAAIDVNMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALLNKP+LIHDILTTL+RNLN  +TCKIRLL S QDTVELARRIE TGVSAL VHGR+
Sbjct: 121 GAALLNKPELIHDILTTLKRNLNSPVTCKIRLLKSSQDTVELARRIEKTGVSALAVHGRK 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           VADRPRDPAKWSEIADVV+AL+IPVIANGD+F+Y DF+RIK  TGASS+MVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVSALSIPVIANGDVFDYSDFERIKTATGASSVMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSPEGK+ +EDVKREYVRKSILWDN+IKSTK+TLKEMIMH+SCLELPEGK VIKSE+LA
Sbjct: 241 IFSPEGKVHYEDVKREYVRKSILWDNNIKSTKHTLKEMIMHYSCLELPEGKGVIKSETLA 300

Query: 301 DLAKLYGEEKYYQFVQENR 320
           D+AKLYGEE YYQFV +NR
Sbjct: 301 DIAKLYGEENYYQFVNDNR 319

[Close]

BLAST of Cucsa.103690 vs. NCBI nr
Match: gi|1009145720|ref|XP_015890488.1| (PREDICTED: tRNA-dihydrouridine(20) synthase [NAD(P)+])

HSP 1 Score: 547.0 bits (1408), Expect = 2.3e-152
Identity = 268/318 (84.28%), Postives = 295/318 (92.77%), Query Frame = 1


Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQHGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDY NK VLAPMVRVGTLPFRLLA+++GADITYGEEIIDHKM+KCERR+NEH+GS DFVE
Sbjct: 1   MDYHNKLVLAPMVRVGTLPFRLLAAEYGADITYGEEIIDHKMLKCERRVNEHIGSIDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGT +VVFRTC+ ERK VVFQ+GTS+A RAL+ AQ+VC DVAA+DINMGCPKSFS+SGGM
Sbjct: 61  KGTESVVFRTCNEERKRVVFQIGTSDALRALTVAQMVCNDVAAVDINMGCPKSFSLSGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALL KPDLIHDILTTLRRNLNI +TCKIRLL SPQDTVEL+RRIE TGVSAL VHGR+
Sbjct: 121 GAALLAKPDLIHDILTTLRRNLNIPVTCKIRLLKSPQDTVELSRRIEKTGVSALAVHGRK 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           VADRPRDPAKWSEIADVVAAL+IPVIANGD+FE+DDF RIK+ TGASS+MVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVAALSIPVIANGDVFEHDDFYRIKLATGASSVMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFS + KLPWEDVKREYVRKSILWDNDIKSTK+TLKEMIMH+SCLEL EGKAVIKS SLA
Sbjct: 241 IFSSQCKLPWEDVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELAEGKAVIKSNSLA 300

Query: 301 DLAKLYGEEKYYQFVQEN 319
           DLA+LYGE+KYY FV  N
Sbjct: 301 DLAQLYGEDKYYDFVTRN 318

The following BLAST results are available for this feature:

BLAST of Cucsa.103690 vs. Swiss-Prot


Match Name	E-value	Identity	Description
DUS2L_HUMAN	3.4e-79	48.01	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like OS=Homo sapiens GN=DUS2 PE=1 SV=...	[more]
DUS2L_MOUSE	1.1e-77	48.89	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like OS=Mus musculus GN=Dus2 PE=1 SV=...	[more]
DUS2_SCHPO	1.7e-51	37.19	tRNA-dihydrouridine(20) synthase [NAD(P)+] OS=Schizosaccharomyces pombe (strain ...	[more]
DUS2_YEAST	3.6e-41	37.25	tRNA-dihydrouridine(20) synthase [NAD(P)+] OS=Saccharomyces cerevisiae (strain A...	[more]
DUS4L_MOUSE	1.6e-28	32.56	tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like OS=Mus musculus GN=Dus4l PE...	[more]

BLAST of Cucsa.103690 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KGL3_CUCSA	6.6e-183	99.69	tRNA-dihydrouridine synthase OS=Cucumis sativus GN=Csa_6G497190 PE=3 SV=1	[more]
A5ALC4_VITVI	1.9e-153	83.70	tRNA-dihydrouridine synthase OS=Vitis vinifera GN=VITISV_024917 PE=3 SV=1	[more]
A0A067KB11_JATCU	5.5e-153	83.39	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_18371 PE=4 SV=1	[more]
V4SKP9_9ROSI	1.3e-151	82.13	tRNA-dihydrouridine synthase OS=Citrus clementina GN=CICLE_v10026068mg PE=3 SV=1	[more]
A0A061DPF2_THECC	3.9e-151	82.45	tRNA-dihydrouridine synthase OS=Theobroma cacao GN=TCM_000905 PE=3 SV=1	[more]

BLAST of Cucsa.103690 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G49640.1	1.2e-138	73.73	Aldolase-type TIM barrel family protein	[more]
AT5G67220.1	5.4e-27	31.17	FMN-linked oxidoreductases superfamily protein	[more]

BLAST of Cucsa.103690 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449451357\|ref\|XP_004143428.1\|	9.5e-183	99.69	PREDICTED: tRNA-dihydrouridine(20) synthase [NAD(P)+]	[more]
gi\|659079878\|ref\|XP_008440492.1\|	2.2e-179	97.54	PREDICTED: tRNA-dihydrouridine(20) synthase [NAD(P)+]	[more]
gi\|147797460\|emb\|CAN64677.1\|	2.7e-153	83.70	hypothetical protein VITISV_024917 [Vitis vinifera]	[more]
gi\|802681614\|ref\|XP_012082133.1\|	7.8e-153	83.39	PREDICTED: tRNA-dihydrouridine(20) synthase [NAD(P)+]	[more]
gi\|1009145720\|ref\|XP_015890488.1\|	2.3e-152	84.28	PREDICTED: tRNA-dihydrouridine(20) synthase [NAD(P)+]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001269	tRNA_hU_synthase
IPR013785	Aldolase_TIM
IPR018517	tRNA_hU_synthase_CS

Vocabulary: **Biological Process**
Term	Definition
GO:0008033	tRNA processing
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0017150	tRNA dihydrouridine synthase activity
GO:0050660	flavin adenine dinucleotide binding
GO:0003824	catalytic activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0002943	tRNA dihydrouridine synthesis
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0017150	tRNA dihydrouridine synthase activity
molecular_function	GO:0003824	catalytic activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.103690.1	Cucsa.103690.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001269	tRNA-dihydrouridine synthase	PIR	PIRSF006621	Dus	coord: 1..318 score: 4.6
IPR001269	tRNA-dihydrouridine synthase	PANTHER	PTHR11082	TRNA-DIHYDROURIDINE SYNTHASE	coord: 1..325 score: 4.5E
IPR001269	tRNA-dihydrouridine synthase	PFAM	PF01207	Dus	coord: 8..256 score: 5.8
IPR013785	Aldolase-type TIM barrel	GENE3D	G3DSA:3.20.20.70		coord: 2..245 score: 6.3
IPR018517	tRNA-dihydrouridine synthase, conserved site	PROSITE	PS01136	UPF0034	coord: 104..122 scor
None	No IPR available	PANTHER	PTHR11082:SF4	TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE	coord: 1..325 score: 4.5E
None	No IPR available	unknown	SSF51395	FMN-linked oxidoreductases	coord: 5..281 score: 1.34

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.103690	Cla014079	Watermelon (97103) v1	cgywmB136
Cucsa.103690	Csa6G497190	Cucumber (Chinese Long) v2	cgycuB121
Cucsa.103690	MELO3C007688	Melon (DHL92) v3.5.1	cgymeB131
Cucsa.103690	ClCG01G018110	Watermelon (Charleston Gray)	cgywcgB134
Cucsa.103690	CSPI06G29430	Wild cucumber (PI 183967)	cgycpiB122
Cucsa.103690	CmaCh07G005990	Cucurbita maxima (Rimu)	cgycmaB0235
Cucsa.103690	CmoCh07G006040	Cucurbita moschata (Rifu)	cgycmoB0233
Cucsa.103690	Lsi01G008380	Bottle gourd (USVL1VR-Ls)	cgylsiB125
Cucsa.103690	Cp4.1LG19g10310	Cucurbita pepo (Zucchini)	cgycpeB0220
Cucsa.103690	MELO3C007688.2	Melon (DHL92) v3.6.1	cgymedB131
Cucsa.103690	CsaV3_6G044650	Cucumber (Chinese Long) v3	cgycucB126
Cucsa.103690	Cla97C01G017360	Watermelon (97103) v2	cgywmbB127
Cucsa.103690	Bhi03G000853	Wax gourd	cgywgoB151
Cucsa.103690	Carg18505	Silver-seed gourd	carcgyB0001

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.103690	Silver-seed gourd	carcgyB0065
Cucsa.103690	Silver-seed gourd	carcgyB0467
Cucsa.103690	Cucumber (Chinese Long) v3	cgycucB118
Cucsa.103690	Cucumber (Chinese Long) v3	cgycucB122
Cucsa.103690	Watermelon (97103) v2	cgywmbB128
Cucsa.103690	Watermelon (97103) v2	cgywmbB135
Cucsa.103690	Wax gourd	cgywgoB157
Cucsa.103690	Cucumber (Gy14) v1	cgycgyB043
Cucsa.103690	Cucumber (Gy14) v1	cgycgyB047
Cucsa.103690	Cucurbita maxima (Rimu)	cgycmaB0222
Cucsa.103690	Cucurbita maxima (Rimu)	cgycmaB0231
Cucsa.103690	Cucurbita maxima (Rimu)	cgycmaB0233
Cucsa.103690	Cucurbita moschata (Rifu)	cgycmoB0227
Cucsa.103690	Cucurbita moschata (Rifu)	cgycmoB0230
Cucsa.103690	Wild cucumber (PI 183967)	cgycpiB116
Cucsa.103690	Wild cucumber (PI 183967)	cgycpiB120
Cucsa.103690	Cucumber (Chinese Long) v2	cgycuB114
Cucsa.103690	Cucumber (Chinese Long) v2	cgycuB119
Cucsa.103690	Melon (DHL92) v3.5.1	cgymeB127
Cucsa.103690	Melon (DHL92) v3.5.1	cgymeB129
Cucsa.103690	Watermelon (Charleston Gray)	cgywcgB133
Cucsa.103690	Watermelon (97103) v1	cgywmB141
Cucsa.103690	Cucurbita pepo (Zucchini)	cgycpeB0211
Cucsa.103690	Cucurbita pepo (Zucchini)	cgycpeB0212
Cucsa.103690	Bottle gourd (USVL1VR-Ls)	cgylsiB127
Cucsa.103690	Bottle gourd (USVL1VR-Ls)	cgylsiB131
Cucsa.103690	Melon (DHL92) v3.6.1	cgymedB126
Cucsa.103690	Melon (DHL92) v3.6.1	cgymedB128