Csa6G497190 (gene) Cucumber (Chinese Long) v2

BLAST of Csa6G497190 vs. Swiss-Prot
Match: DUS2L_HUMAN (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like OS=Homo sapiens GN=DUS2 PE=1 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 8.8e-80
Identity = 158/327 (48.32%), Postives = 215/327 (65.75%), Query Frame = 1

Query: 3   YENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVEKG 62
           Y NK +LAPMVRVGTLP RLLA  YGADI Y EE+ID KMI+C+R +NE + + DFV   
Sbjct: 10  YHNKLILAPMVRVGTLPMRLLALDYGADIVYCEELIDLKMIQCKRVVNEVLSTVDFVAPD 69

Query: 63  TNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGMGA 122
            + VVFRTC  E+  VVFQMGTS+A RAL+ A+LV  DVA ID+NMGCPK +S  GGMGA
Sbjct: 70  -DRVVFRTCEREQNRVVFQMGTSDAERALAVARLVENDVAGIDVNMGCPKQYSTKGGMGA 129

Query: 123 ALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRRVA 182
           ALL+ PD I  IL+TL +     +TCKIR+L S +DT+ L +RIE TG++A+ VHGR+  
Sbjct: 130 ALLSDPDKIEKILSTLVKGTRRPVTCKIRILPSLEDTLSLVKRIERTGIAAIAVHGRKRE 189

Query: 183 DRPRDPAKWSEIADVVAALTIPVIANG----DIFEYDDFQRIKVHTGASSMMVARGALWN 242
           +RP+ P     I  +   L+IPVIANG     I +Y D +  +  T ASS+MVAR A+WN
Sbjct: 190 ERPQHPVSCEVIKAIADTLSIPVIANGGSHDHIQQYSDIEDFRQATAASSVMVARAAMWN 249

Query: 243 ASIFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSES 302
            SIF  EG  P E+V ++Y+R ++ +DN   +TKY L +M+     LE P+G+ +  ++S
Sbjct: 250 PSIFLKEGLRPLEEVMQKYIRYAVQYDNHYTNTKYCLCQMLREQ--LESPQGRLLHAAQS 309

Query: 303 LADLAKLYGEEKYYQFVQENRSFNESR 326
             ++ + +G   +Y+   +     ++R
Sbjct: 310 SREICEAFGLGAFYEETTQELDAQQAR 333

BLAST of Csa6G497190 vs. Swiss-Prot
Match: DUS2L_MOUSE (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like OS=Mus musculus GN=Dus2 PE=1 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 2.8e-78
Identity = 155/315 (49.21%), Postives = 211/315 (66.98%), Query Frame = 1

Query: 3   YENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVEKG 62
           Y NK +LAPMVRVGTLP RLLA  YGADI Y EE+ID KM++C+R +NE + + DFV   
Sbjct: 10  YHNKLILAPMVRVGTLPMRLLALDYGADIVYCEELIDLKMLQCKRVVNEVLSTVDFVAPD 69

Query: 63  TNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGMGA 122
            + VVFRTC  E+  VVFQMGTS+A RAL+ A+LV  DVA ID+NMGCPK +S  GGMGA
Sbjct: 70  -DRVVFRTCEREQSRVVFQMGTSDAERALAVARLVENDVAGIDVNMGCPKEYSTKGGMGA 129

Query: 123 ALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRRVA 182
           ALL+ PD I  IL+TL +  +  +TCKIR+L S +DT+ L +RIE TG+SA+ VHGR   
Sbjct: 130 ALLSDPDKIEKILSTLVKGTHRPVTCKIRILPSLEDTLNLVKRIERTGISAIAVHGRNRD 189

Query: 183 DRPRDPAKWSEIADVVAALTIPVIANG----DIFEYDDFQRIKVHTGASSMMVARGALWN 242
           +RP+ P     I  +   L+IPVIANG     I ++ D +  +  T ASS+MVAR A+WN
Sbjct: 190 ERPQHPVSCEVIRAIAETLSIPVIANGGSHDHIQQHVDIEDFRQATAASSVMVARAAMWN 249

Query: 243 ASIFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSES 302
            SIF  +G  P E+V ++Y+R ++ +DN   +TKY L +M+     LE P+G+ +  ++S
Sbjct: 250 PSIFLKDGLRPLEEVMQKYIRYAVQYDNHYTNTKYCLCQMLREQ--LESPQGRLLHAAQS 309

Query: 303 LADLAKLYGEEKYYQ 314
             ++ + +G   +Y+
Sbjct: 310 SQEICEAFGLGAFYE 321

BLAST of Csa6G497190 vs. Swiss-Prot
Match: DUS2_SCHPO (tRNA-dihydrouridine(20) synthase [NAD(P)+] OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=dus2 PE=3 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 4.6e-52
Identity = 120/320 (37.50%), Postives = 178/320 (55.62%), Query Frame = 1

Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIK---CERRLNEHVGSTD 60
           ++Y NK  LAPMVR+G LP RLLA +YGA++ +G EI+D  ++     ER +N+ +   D
Sbjct: 4   LNYSNKVCLAPMVRIGELPMRLLALRYGANLVWGPEIVDKALLSGTPVERVVNDRINCID 63

Query: 61  FVEKGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSIS 120
           FV+  +N V+FR   LE   ++FQ+G+++   A+ AA+LV  DVA ID+N GCPK FS+ 
Sbjct: 64  FVKPPSNKVLFRVHPLEANRLIFQLGSASPELAVEAAKLVANDVAGIDLNCGCPKHFSVH 123

Query: 121 GGMGAALLNKPDLIHDILTTLRRNL----NILITCKIRLLNSPQDTVELARRIESTGVSA 180
            GMGA LL   D +  IL  L   +     I I+CKIRLL + +DT++L  RI  TGV A
Sbjct: 124 AGMGAGLLKNQDRLVSILDALVNEIGKPYKISISCKIRLLETKEDTLKLVERICDTGVRA 183

Query: 181 LGVHGRRVADRPRDPAKWSEIADVVAAL---TIPVIANGDIFEYDDFQRIKVHTGASSMM 240
           + VH R    R  +PA  S ++++V       + ++ NGD+  Y+D   +    G   ++
Sbjct: 184 ITVHCRTTPMRNTEPADRSYLSEIVGVCRNKDVSILVNGDVLSYNDGLDVIEKYGVDGVL 243

Query: 241 VARGALWNASIFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEG 300
           +AR A  N S F  EG L    V  E+++ ++  DN+  +TKY L +++         +G
Sbjct: 244 IARAAERNVSCFRIEGPLSSFKVAEEFLKMALEVDNNFGNTKYCLNQIM---------QG 303

Query: 301 KAVIKSESLADLAKLYGEEK 311
                   LA  AK Y + K
Sbjct: 304 SFRKNVRQLAQTAKTYEDLK 314

BLAST of Csa6G497190 vs. Swiss-Prot
Match: DUS2_YEAST (tRNA-dihydrouridine(20) synthase [NAD(P)+] OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SMM1 PE=1 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.8e-40
Identity = 110/298 (36.91%), Postives = 167/298 (56.04%), Query Frame = 1

Query: 3   YENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFV--- 62
           Y  K VLAPMVR G LP RL+A  +GAD+ +  EIID K+I+C R+ N  + + D+V   
Sbjct: 4   YAGKLVLAPMVRAGELPTRLMALAHGADLVWSPEIIDKKLIQCVRKENTALQTVDYVVPS 63

Query: 63  --EKGTNNVVFRTC-SLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSI 122
             +     +VFRT   LE   ++FQ+G+++ A A  AA  V  DV+ IDIN GCPK FSI
Sbjct: 64  KVQTRPETLVFRTYPKLESSKLIFQIGSASPALATQAALKVINDVSGIDINAGCPKHFSI 123

Query: 123 SGGMGAALLNKPDLIHDILTTLRRNL----NILITCKIRLLNSPQDTVELARRIESTGVS 182
             GMG+ALL  PD +  IL  L +N+    +  I+ KIRLL++ QDT++L +R+ +TG++
Sbjct: 124 HSGMGSALLRTPDTLCLILKELVKNVGNPHSKPISVKIRLLDTKQDTLQLVKRLCATGIT 183

Query: 183 ALGVHGRRVADRPRDPA---KWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSM 242
            L VH R+   R R+       +EI ++  A  + +I NG I +   F  ++ +   ++ 
Sbjct: 184 NLTVHCRKTEMRNREQPITDYIAEIYEICQANNVSLIVNGAIRDRSHFHDLQANHWKNTN 243

Query: 243 ---MVARGALWNASIFSPEGK-----LPWEDVKREYVRKSILWDNDIKSTKYTLKEMI 280
              M+A  A  + ++F    K       W    RE+++ +  +DN I +TKY L  ++
Sbjct: 244 IGGMIAECAERDPTVFDHTSKPSEDGPSWVVACREFIQWATKFDNHIGNTKYMLSRIV 301

BLAST of Csa6G497190 vs. Swiss-Prot
Match: DUS4L_MOUSE (tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like OS=Mus musculus GN=Dus4l PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 4.2e-29
Identity = 85/258 (32.95%), Postives = 129/258 (50.00%), Query Frame = 1

Query: 8   VLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVEKGTNNVV 67
           V APMVR   L FR L  +Y  D+ Y   II    ++  +  +         E  TN   
Sbjct: 30  VCAPMVRYSKLAFRTLVRKYSCDLCYTPMIIAADFVRSIKARDS--------EFTTNQ-- 89

Query: 68  FRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGMGAALLNK 127
              C L     + Q   ++A     AA LVC     IDIN GCP+ ++++ G GA L+NK
Sbjct: 90  -GDCPL-----IVQFAANDARLLSDAALLVCPYANGIDINCGCPQRWAMADGYGACLINK 149

Query: 128 PDLIHDILTTLRRNL---NILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRRVADR 187
           P+L+HD++  +R  +      ++ KIR+ +    T++L R+ E+TGVS + VHGR V +R
Sbjct: 150 PELVHDMVRQVRNRVESPRFSVSIKIRIHDDLARTIDLCRKAEATGVSWITVHGRTVEER 209

Query: 188 PRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNASIFSP 247
              P  +  I  +   ++IP++ANGDI    + + +   TG   +MVARG L N ++F+ 
Sbjct: 210 -HQPVHYDAIKMIKENVSIPIVANGDIRSLKEAENVWQMTGTDGVMVARGLLANPAMFAG 269

Query: 248 EGKLP------WEDVKRE 257
             + P      W D+  E
Sbjct: 270 YEETPLKCIWDWVDISLE 270

BLAST of Csa6G497190 vs. TrEMBL
Match: A5ALC4_VITVI (tRNA-dihydrouridine synthase OS=Vitis vinifera GN=VITISV_024917 PE=3 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 4.9e-154
Identity = 268/319 (84.01%), Postives = 299/319 (93.73%), Query Frame = 1

Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           M+Y N+ VLAPMVRVGTLPFRLLA+QYGADITYGEEIIDHK+IKCERRLNE +GS DFVE
Sbjct: 1   MEYGNRLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKLIKCERRLNEDIGSIDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGT+NVVFRTC  E+  VVFQMGTSNA RAL+AAQ+VC+DVAA+DINMGCPKSFSISGGM
Sbjct: 61  KGTHNVVFRTCHEEKNRVVFQMGTSNAERALTAAQIVCKDVAAVDINMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALL +P+LIHDILT L+RNL+I +TCKIRLL S Q+TVELA++IE TGV+AL VHGRR
Sbjct: 121 GAALLTRPELIHDILTILKRNLDIPVTCKIRLLKSSQETVELAQQIEKTGVAALAVHGRR 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           +ADRPRDPAKW+EIADVVAAL+IPVIANGD+F YDDFQRIKV TGASS+MVARGALWNAS
Sbjct: 181 IADRPRDPAKWNEIADVVAALSIPVIANGDVFGYDDFQRIKVATGASSVMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           +FSPEGK+PWEDVK EY+RKSILWDNDIKSTK+TLKEMIMHHSCLELPEGKAVIKSE+LA
Sbjct: 241 VFSPEGKVPWEDVKXEYIRKSILWDNDIKSTKHTLKEMIMHHSCLELPEGKAVIKSETLA 300

Query: 301 DLAKLYGEEKYYQFVQENR 320
           DLAKLYGEEKYYQFV+ NR
Sbjct: 301 DLAKLYGEEKYYQFVRGNR 319

BLAST of Csa6G497190 vs. TrEMBL
Match: A0A067KB11_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_18371 PE=4 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 1.4e-153
Identity = 267/319 (83.70%), Postives = 299/319 (93.73%), Query Frame = 1

Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           M+YENK VLAPMVRVGTLPFRLLA++YGADITYGEEIIDHKM+KCER++NE++G TDFVE
Sbjct: 1   MEYENKLVLAPMVRVGTLPFRLLAAEYGADITYGEEIIDHKMLKCERKVNEYIGCTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGT NVVFRTC+ ER  VVFQMGTS+A RAL+AAQ+VC+DVAAID+NMGCPKSFSISGGM
Sbjct: 61  KGTENVVFRTCNQERNRVVFQMGTSDAVRALTAAQMVCKDVAAIDVNMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALLNKP+LIHDILTTL+RNLN  +TCKIRLL S QDTVELARRIE TGVSAL VHGR+
Sbjct: 121 GAALLNKPELIHDILTTLKRNLNSPVTCKIRLLKSSQDTVELARRIEKTGVSALAVHGRK 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           VADRPRDPAKWSEIADVV+AL+IPVIANGD+F+Y DF+RIK  TGASS+MVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVSALSIPVIANGDVFDYSDFERIKTATGASSVMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSPEGK+ +EDVKREYVRKSILWDN+IKSTK+TLKEMIMH+SCLELPEGK VIKSE+LA
Sbjct: 241 IFSPEGKVHYEDVKREYVRKSILWDNNIKSTKHTLKEMIMHYSCLELPEGKGVIKSETLA 300

Query: 301 DLAKLYGEEKYYQFVQENR 320
           D+AKLYGEE YYQFV +NR
Sbjct: 301 DIAKLYGEENYYQFVNDNR 319

BLAST of Csa6G497190 vs. TrEMBL
Match: V4SKP9_9ROSI (tRNA-dihydrouridine synthase OS=Citrus clementina GN=CICLE_v10026068mg PE=3 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 3.5e-152
Identity = 263/319 (82.45%), Postives = 298/319 (93.42%), Query Frame = 1

Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDY+NK VLAPMVRVGTLPFRLLA+QYGADITYGEEIIDHK++KCERR+NE++GSTDFVE
Sbjct: 1   MDYQNKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKLLKCERRVNEYIGSTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGT++VVFRTC  ER HVVFQMGTS+A RAL+AA++VC+DVAAIDINMGCPKSFS+SGGM
Sbjct: 61  KGTDSVVFRTCHQERNHVVFQMGTSDAVRALTAAKMVCKDVAAIDINMGCPKSFSVSGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALL+KP+LIHDILT L+RNL++ +TCKIRLL S QDTVELAR+IE TGVSAL VHGR+
Sbjct: 121 GAALLSKPELIHDILTMLKRNLDVPVTCKIRLLKSSQDTVELARQIEKTGVSALAVHGRK 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           V DRPRDPAKW EIAD+VAAL+IPVIANGD+FEYDDFQRIK  TGASS+M ARGALWNAS
Sbjct: 181 VVDRPRDPAKWGEIADIVAALSIPVIANGDVFEYDDFQRIKTATGASSVMAARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFS +GKL WEDVKREYVRKSILW+ND+KSTK+TLKEMIMH+S LELPEGKA+IKSE+LA
Sbjct: 241 IFSSQGKLHWEDVKREYVRKSILWENDVKSTKHTLKEMIMHYSSLELPEGKAIIKSETLA 300

Query: 301 DLAKLYGEEKYYQFVQENR 320
           D+AKLY EEKYYQFV ENR
Sbjct: 301 DIAKLYEEEKYYQFVNENR 319

BLAST of Csa6G497190 vs. TrEMBL
Match: A0A061DPF2_THECC (tRNA-dihydrouridine synthase OS=Theobroma cacao GN=TCM_000905 PE=3 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 1.0e-151
Identity = 264/319 (82.76%), Postives = 296/319 (92.79%), Query Frame = 1

Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDY NK VLAPMVRVGTLPFRLLA+ YGADITY EEIIDHK+IKCER++NE++GSTDFVE
Sbjct: 1   MDYGNKLVLAPMVRVGTLPFRLLAADYGADITYAEEIIDHKLIKCERKVNEYIGSTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGT+NVVFRTC+ ER  VVFQMGTS+A RAL AAQ+VC+DVAA+DINMGCPKSFS+SGGM
Sbjct: 61  KGTDNVVFRTCNQERNQVVFQMGTSDAVRALKAAQIVCKDVAAVDINMGCPKSFSVSGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALL KPDLIHDILTTLRRNL+  +TCKIRLL S QDTVELARRIE TGVSAL VHGR+
Sbjct: 121 GAALLTKPDLIHDILTTLRRNLDTPVTCKIRLLKSSQDTVELARRIEKTGVSALAVHGRK 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           VADRPRDPAKW+EIAD+V+AL+IPVIANGD+FEYDDFQRIKV TGASS+MVARGALWNAS
Sbjct: 181 VADRPRDPAKWNEIADIVSALSIPVIANGDVFEYDDFQRIKVATGASSVMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFS +GK  WEDVKREYVRKSILWDND+KSTK+TL+E+I H+SCLELPEGKAVIKSE+LA
Sbjct: 241 IFSSKGKAHWEDVKREYVRKSILWDNDVKSTKHTLREIITHYSCLELPEGKAVIKSENLA 300

Query: 301 DLAKLYGEEKYYQFVQENR 320
           DLAK+YGEE+YY+ V ENR
Sbjct: 301 DLAKVYGEEEYYRLVSENR 319

BLAST of Csa6G497190 vs. TrEMBL
Match: B9SSD6_RICCO (tRNA-dihydrouridine synthase OS=Ricinus communis GN=RCOM_0188130 PE=3 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 1.3e-151
Identity = 261/319 (81.82%), Postives = 296/319 (92.79%), Query Frame = 1

Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           M+YENK VLAPMVRVGTLPFRLLA++YGADITYGEEIIDHK++KCER++NE++G TDFVE
Sbjct: 1   MEYENKLVLAPMVRVGTLPFRLLAAEYGADITYGEEIIDHKLLKCERKVNEYIGCTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGT NVVFRTC+ E+  VVFQMGTS+A RAL+AAQ+VC+DVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTENVVFRTCNEEKNRVVFQMGTSDAVRALTAAQMVCKDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALL KP+LIHDILTTL+RNL+  +TCKIRLL SPQDTVELARRIE TGVSAL VHGR+
Sbjct: 121 GAALLTKPELIHDILTTLKRNLDSPVTCKIRLLKSPQDTVELARRIEKTGVSALAVHGRK 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           VADRPRDPAKWSEIAD+V+ L+IPVIANGD+F+Y DF+RIK  TGASS+MVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADIVSTLSIPVIANGDVFDYGDFERIKAATGASSVMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSP+GK+ WEDVKREYVRKSILWDND+KSTK+TLKEMIMH+SCLELPEGK VIKSE+LA
Sbjct: 241 IFSPKGKVHWEDVKREYVRKSILWDNDVKSTKHTLKEMIMHYSCLELPEGKGVIKSETLA 300

Query: 301 DLAKLYGEEKYYQFVQENR 320
           D+AK YGEE+YYQ V E R
Sbjct: 301 DIAKFYGEERYYQLVNEKR 319

BLAST of Csa6G497190 vs. TAIR10
Match: AT3G49640.1 (AT3G49640.1 Aldolase-type TIM barrel family protein)

HSP 1 Score: 491.9 bits (1265), Expect = 3.1e-139
Identity = 234/316 (74.05%), Postives = 276/316 (87.34%), Query Frame = 1

Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDY+NK VLAPMVRVGTL FR+LA++YGADITYGEEIIDHK++KCERRLN   G+++FVE
Sbjct: 1   MDYQNKLVLAPMVRVGTLSFRMLAAEYGADITYGEEIIDHKLVKCERRLNVASGTSEFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGT+NVVF TC  E+  VVFQMGTS+A RAL A+++VC DVA IDINMGCPK+FSI GGM
Sbjct: 61  KGTDNVVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALL+KP+LIHDIL TL+RNL++ +TCKIRLL SP DTVELARRIE  GV AL VHGR+
Sbjct: 121 GAALLSKPELIHDILATLKRNLDVPVTCKIRLLKSPADTVELARRIEKLGVPALAVHGRK 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           +ADRPRDPAKW EIADVVAAL+IPVIANGD+ EYDDF RIK  TGA+S+MVARGA+WNAS
Sbjct: 181 IADRPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATGAASVMVARGAMWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSP+GK  WEDVK++Y+RKSILW+ND+KSTKYT+KEMI HHSCLELPEGK++ K+++L 
Sbjct: 241 IFSPKGKSHWEDVKKKYLRKSILWNNDVKSTKYTIKEMIAHHSCLELPEGKSINKADTLE 300

Query: 301 DLAKLYGEEKYYQFVQ 317
           DLA+LY  E Y+  V+
Sbjct: 301 DLARLYDLEDYFWTVK 316

BLAST of Csa6G497190 vs. TAIR10
Match: AT5G67220.1 (AT5G67220.1 FMN-linked oxidoreductases superfamily protein)

HSP 1 Score: 120.9 bits (302), Expect = 1.4e-27
Identity = 78/247 (31.58%), Postives = 119/247 (48.18%), Query Frame = 1

Query: 6   KFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVEKGTNN 65
           K+++APMV    LPFRLL  +YGA   Y   +      + E+  N+              
Sbjct: 89  KYIVAPMVDNSELPFRLLCQKYGAQAAYTPMLHSRIFTETEKYRNQE------------- 148

Query: 66  VVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGMGAALL 125
             F TC  +R   V Q   ++    L AA+ V      +DIN+GCP+  +  G  GA L+
Sbjct: 149 --FTTCKEDRPLFV-QFCANDPDTLLEAAKRVEPYCDYVDINLGCPQRIARRGNYGAFLM 208

Query: 126 NKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR--VAD 185
           +   L+  ++  L +NLN+ ++CKIR+  + +DT++ A+ +E  G S L VHGR     D
Sbjct: 209 DNLPLVKSLVEKLAQNLNVPVSCKIRIFPNLEDTLKYAKMLEDAGCSLLAVHGRTRDEKD 268

Query: 186 RPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNASIFS 245
             +  A WS I +V  A+ IPV+ANG++   +D       TG   ++ A   L N + F+
Sbjct: 269 GKKFRADWSAIKEVKNAMRIPVLANGNVRCIEDVDNCIKETGVEGVLSAETLLENPAAFA 319

Query: 246 PEGKLPW 251
                 W
Sbjct: 329 GFRTAEW 319

BLAST of Csa6G497190 vs. NCBI nr
Match: gi|449451357|ref|XP_004143428.1| (PREDICTED: tRNA-dihydrouridine(20) synthase [NAD(P)+])

HSP 1 Score: 649.8 bits (1675), Expect = 2.5e-183
Identity = 325/325 (100.00%), Postives = 325/325 (100.00%), Query Frame = 1

Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDYENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE
Sbjct: 1   MDYENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR
Sbjct: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA
Sbjct: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300

Query: 301 DLAKLYGEEKYYQFVQENRSFNESR 326
           DLAKLYGEEKYYQFVQENRSFNESR
Sbjct: 301 DLAKLYGEEKYYQFVQENRSFNESR 325

BLAST of Csa6G497190 vs. NCBI nr
Match: gi|659079878|ref|XP_008440492.1| (PREDICTED: tRNA-dihydrouridine(20) synthase [NAD(P)+])

HSP 1 Score: 638.6 bits (1646), Expect = 5.7e-180
Identity = 318/325 (97.85%), Postives = 323/325 (99.38%), Query Frame = 1

Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDYENK VLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE
Sbjct: 1   MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVC+DVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALLNKPDLIHDILTTLRRNL++LITCKIRLLNSPQDTVELARRIE+TGVSALGVHGRR
Sbjct: 121 GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASS+MVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA
Sbjct: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300

Query: 301 DLAKLYGEEKYYQFVQENRSFNESR 326
           DLAKLYGEEKYYQFVQENRSFN SR
Sbjct: 301 DLAKLYGEEKYYQFVQENRSFNGSR 325

BLAST of Csa6G497190 vs. NCBI nr
Match: gi|147797460|emb|CAN64677.1| (hypothetical protein VITISV_024917 [Vitis vinifera])

HSP 1 Score: 552.0 bits (1421), Expect = 7.1e-154
Identity = 268/319 (84.01%), Postives = 299/319 (93.73%), Query Frame = 1

Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           M+Y N+ VLAPMVRVGTLPFRLLA+QYGADITYGEEIIDHK+IKCERRLNE +GS DFVE
Sbjct: 1   MEYGNRLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKLIKCERRLNEDIGSIDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGT+NVVFRTC  E+  VVFQMGTSNA RAL+AAQ+VC+DVAA+DINMGCPKSFSISGGM
Sbjct: 61  KGTHNVVFRTCHEEKNRVVFQMGTSNAERALTAAQIVCKDVAAVDINMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALL +P+LIHDILT L+RNL+I +TCKIRLL S Q+TVELA++IE TGV+AL VHGRR
Sbjct: 121 GAALLTRPELIHDILTILKRNLDIPVTCKIRLLKSSQETVELAQQIEKTGVAALAVHGRR 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           +ADRPRDPAKW+EIADVVAAL+IPVIANGD+F YDDFQRIKV TGASS+MVARGALWNAS
Sbjct: 181 IADRPRDPAKWNEIADVVAALSIPVIANGDVFGYDDFQRIKVATGASSVMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           +FSPEGK+PWEDVK EY+RKSILWDNDIKSTK+TLKEMIMHHSCLELPEGKAVIKSE+LA
Sbjct: 241 VFSPEGKVPWEDVKXEYIRKSILWDNDIKSTKHTLKEMIMHHSCLELPEGKAVIKSETLA 300

Query: 301 DLAKLYGEEKYYQFVQENR 320
           DLAKLYGEEKYYQFV+ NR
Sbjct: 301 DLAKLYGEEKYYQFVRGNR 319

BLAST of Csa6G497190 vs. NCBI nr
Match: gi|802681614|ref|XP_012082133.1| (PREDICTED: tRNA-dihydrouridine(20) synthase [NAD(P)+])

HSP 1 Score: 550.4 bits (1417), Expect = 2.1e-153
Identity = 267/319 (83.70%), Postives = 299/319 (93.73%), Query Frame = 1

Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           M+YENK VLAPMVRVGTLPFRLLA++YGADITYGEEIIDHKM+KCER++NE++G TDFVE
Sbjct: 1   MEYENKLVLAPMVRVGTLPFRLLAAEYGADITYGEEIIDHKMLKCERKVNEYIGCTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGT NVVFRTC+ ER  VVFQMGTS+A RAL+AAQ+VC+DVAAID+NMGCPKSFSISGGM
Sbjct: 61  KGTENVVFRTCNQERNRVVFQMGTSDAVRALTAAQMVCKDVAAIDVNMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALLNKP+LIHDILTTL+RNLN  +TCKIRLL S QDTVELARRIE TGVSAL VHGR+
Sbjct: 121 GAALLNKPELIHDILTTLKRNLNSPVTCKIRLLKSSQDTVELARRIEKTGVSALAVHGRK 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           VADRPRDPAKWSEIADVV+AL+IPVIANGD+F+Y DF+RIK  TGASS+MVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVSALSIPVIANGDVFDYSDFERIKTATGASSVMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSPEGK+ +EDVKREYVRKSILWDN+IKSTK+TLKEMIMH+SCLELPEGK VIKSE+LA
Sbjct: 241 IFSPEGKVHYEDVKREYVRKSILWDNNIKSTKHTLKEMIMHYSCLELPEGKGVIKSETLA 300

Query: 301 DLAKLYGEEKYYQFVQENR 320
           D+AKLYGEE YYQFV +NR
Sbjct: 301 DIAKLYGEENYYQFVNDNR 319

BLAST of Csa6G497190 vs. NCBI nr
Match: gi|1009145720|ref|XP_015890488.1| (PREDICTED: tRNA-dihydrouridine(20) synthase [NAD(P)+])

HSP 1 Score: 548.9 bits (1413), Expect = 6.0e-153
Identity = 269/318 (84.59%), Postives = 295/318 (92.77%), Query Frame = 1

Query: 1   MDYENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDY NK VLAPMVRVGTLPFRLLA++YGADITYGEEIIDHKM+KCERR+NEH+GS DFVE
Sbjct: 1   MDYHNKLVLAPMVRVGTLPFRLLAAEYGADITYGEEIIDHKMLKCERRVNEHIGSIDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120
           KGT +VVFRTC+ ERK VVFQ+GTS+A RAL+ AQ+VC DVAA+DINMGCPKSFS+SGGM
Sbjct: 61  KGTESVVFRTCNEERKRVVFQIGTSDALRALTVAQMVCNDVAAVDINMGCPKSFSLSGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180
           GAALL KPDLIHDILTTLRRNLNI +TCKIRLL SPQDTVEL+RRIE TGVSAL VHGR+
Sbjct: 121 GAALLAKPDLIHDILTTLRRNLNIPVTCKIRLLKSPQDTVELSRRIEKTGVSALAVHGRK 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240
           VADRPRDPAKWSEIADVVAAL+IPVIANGD+FE+DDF RIK+ TGASS+MVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVAALSIPVIANGDVFEHDDFYRIKLATGASSVMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFS + KLPWEDVKREYVRKSILWDNDIKSTK+TLKEMIMH+SCLEL EGKAVIKS SLA
Sbjct: 241 IFSSQCKLPWEDVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELAEGKAVIKSNSLA 300

Query: 301 DLAKLYGEEKYYQFVQEN 319
           DLA+LYGE+KYY FV  N
Sbjct: 301 DLAQLYGEDKYYDFVTRN 318