Cucsa.099120 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.099120 vs. Swiss-Prot
Match: MSL6_ARATH (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana GN=MSL6 PE=1 SV=1)

HSP 1 Score: 906.7 bits (2342), Expect = 1.8e-262
Identity = 466/809 (57.60%), Postives = 586/809 (72.44%), Query Frame = 1

Query: 2   EDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRIVPPLAESPLSQTSKDLKVSFQHD 61
           EDPPTKLIG FL KQ+  GE  LDMDL M+EL+ +R + P++ESP   T +D        
Sbjct: 82  EDPPTKLIGQFLHKQQASGEICLDMDLGMDELQ-SRGLTPVSESPRVSTKRD-------- 141

Query: 62  STEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQNESLAEAMRCA 121
              +   D      N D  ++ + S G  +P Q               ++ S    MR  
Sbjct: 142 --PVGRRDSRSNTNNNDDGEVVKCS-GNNAPIQ---------------RSSSTLLKMRTR 201

Query: 122 SNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSGQLRSGFLGKIEDDDDD 181
           S LS         ++    A  KS  I  P      SG   KS + +       E+++DD
Sbjct: 202 SRLSDPPTPQLPPQT----ADMKSGRI--PKSGQMKSGFFGKSPKTQG------EEEEDD 261

Query: 182 PFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMI 241
           PF  +DLP+E+++   S   VL+W+SLI+I A  +CTL++P LR+K LWEL +WKWE M+
Sbjct: 262 PFAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMV 321

Query: 242 FILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFN 301
            +LICGRLVS W ++I VFFIERNFLLRKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+
Sbjct: 322 LVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFD 381

Query: 302 KRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQY 361
           ++V K  NT  L  V+++ VCLL+  L+WLVKTL+VKVLASSFH+STYFDRIQESLF QY
Sbjct: 382 EKVAKAANTKALRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQY 441

Query: 362 VIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASIKSGREIGSGRT 421
           VIETLSGPPL+EI+KNEEEEERI+ EV+K QN G +            I+SG +    +T
Sbjct: 442 VIETLSGPPLIEIQKNEEEEERISVEVKKFQNPGGV-----------EIQSGAQKSPMKT 501

Query: 422 HKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQI 481
            KS        +       N GITID LHKL+ KNVSAW MKRL+NI+R GS++TLDEQ+
Sbjct: 502 GKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQL 561

Query: 482 RGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFE 541
           + P LDD+   +I+SE EAK AA+KIF NVA  G K+IY +D++RF+ +DE LKT+SLFE
Sbjct: 562 QDPSLDDDKGNQIRSEFEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFE 621

Query: 542 GAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIV 601
           GA+E  RISKS+LKNWVVNAFRERRALALTLNDTKTAV++LH MVN++ GI+IL++WLI+
Sbjct: 622 GASETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLII 681

Query: 602 LGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEE 661
           LGI S+KF + +SSQ+VVVAFIFGN CK +FE+II+LFV+HPFDVGDRCEIDG+QMVVEE
Sbjct: 682 LGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEE 741

Query: 662 MNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHR 721
           MNILTTVFLR+DN KV+ PNS+L TK I N+YRSPDMG+ IEF +HI TPAEKI  +K R
Sbjct: 742 MNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQR 801

Query: 722 IISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVK 781
           I SYIEG K+HWYPAPMIVFKD++ LN V++AVW +HRMNHQD GE+WARRS LVEE+ K
Sbjct: 802 ITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAK 840

Query: 782 VCQELDIQYRLLPIDINIRSLPSSNTITI 811
           +C+ELDI+YRL P+DIN+R+LP+S  + +
Sbjct: 862 ICRELDIEYRLYPLDINVRNLPTSTALPV 840

BLAST of Cucsa.099120 vs. Swiss-Prot
Match: MSL8_ARATH (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana GN=MSL8 PE=2 SV=2)

HSP 1 Score: 902.9 bits (2332), Expect = 2.6e-261
Identity = 461/826 (55.81%), Postives = 613/826 (74.21%), Query Frame = 1

Query: 1   MEDPPTKLI-GDFLRKQ---KIEGETTLDMDLEMEELKPNRIVPPLAESPLS-QTSKDLK 60
           +++ PTK++ G+ + +Q   +   E TLD+D E +++    +  P + +  S   S++++
Sbjct: 118 VDESPTKMVAGEPVNRQWRGRNNEEITLDVDQENDDVSHQTMPTPTSTARTSFDASREMR 177

Query: 61  VSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQNESLA 120
           VSF            ++RR              G  P    H     S T+   Q++   
Sbjct: 178 VSF------------NVRRAGG-------AFVAGSVPSSSSHSSSSSSATMRTNQDQPQL 237

Query: 121 ---EAMRCASNLSFHSELSFQRKSNLL-RAKTKSRLIDPPAEPDR-LSGLIPKSGQLRSG 180
              E +RC SN+SF      QRKS L+ R KT+SRL DPP E +   SG   +SGQL+SG
Sbjct: 238 QEEEVVRCTSNMSF------QRKSELISRVKTRSRLQDPPREEETPYSGW--RSGQLKSG 297

Query: 181 FLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLW 240
            L  I D++DDP  E+D+PDE+KRG   A+T+LQW+SL+ I AAL C+LS+   ++  +W
Sbjct: 298 LLADI-DEEDDPLAEEDVPDEYKRGKLDAITLLQWLSLVAIIAALACSLSIQSWKKVRVW 357

Query: 241 ELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLG 300
            L +WKWEV + +LICGRLVSGWGIRI VFFIERNFLLRKRVLYFVYGVR+ VQNCLWLG
Sbjct: 358 NLHLWKWEVFLLVLICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVYGVRRAVQNCLWLG 417

Query: 301 LVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYF 360
           LVL+AWH LF+K+V+++T +  L YV+++LVC L+ST++WL+KTL+VKVLASSFHVSTYF
Sbjct: 418 LVLLAWHFLFDKKVQRETRSRFLPYVTKILVCFLLSTILWLIKTLVVKVLASSFHVSTYF 477

Query: 361 DRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASI 420
           DRIQE+LFNQYVIETLSGPP++E+ + EEEEER  DE+ K+QNAG  +PPDL AA F   
Sbjct: 478 DRIQEALFNQYVIETLSGPPMIEMSRIEEEEERAQDEIFKMQNAGANLPPDLCAAAFPPG 537

Query: 421 KSGREIGSGRTHKSFCAKSCKLSRALTKNRND-GITIDHLHKLSTKNVSAWNMKRLLNIV 480
           KSGR +              KLS  + K+  D GI+++HLH+++ KN+SAWNMKRL+ IV
Sbjct: 538 KSGRVMNP------------KLSPIIPKSTTDNGISMEHLHRMNHKNISAWNMKRLMKIV 597

Query: 481 RYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMR 540
           R  S++TLDEQ+     +DEST +I+SE+EAKAAA+KIF+NV  RG KYIYL+DL+RF+R
Sbjct: 598 RNVSLTTLDEQMLESTYEDESTRQIRSEKEAKAAARKIFKNVEQRGAKYIYLEDLMRFLR 657

Query: 541 EDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVI 600
           EDE +KTM LFEGA EN+RISKSALKNW+VNAFRERRALALTLNDTKTAV+KLHHM+N++
Sbjct: 658 EDEAMKTMGLFEGAPENKRISKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMINIV 717

Query: 601 FGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDR 660
             I+I+++WL++L IASSK  +F+SSQ+V++AFIFGNT KT+FE+IIFLF++HP+DVGDR
Sbjct: 718 TAIVIVVIWLVLLEIASSKVLLFVSSQVVLLAFIFGNTVKTVFESIIFLFIVHPYDVGDR 777

Query: 661 CEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIA 720
           CEID +Q+VVEEMNILTTVFLRYDNLK++ PNS+L  K I+N+YRSPDMG++IEF VHI 
Sbjct: 778 CEIDSVQLVVEEMNILTTVFLRYDNLKIMYPNSLLWQKSINNYYRSPDMGDAIEFCVHIT 837

Query: 721 TPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERW 780
           TP EKI+ +K RI +YI+   E+WYP   I+ KD++ L+ V+LA+W  HR+NHQD  ERW
Sbjct: 838 TPLEKISVIKQRISNYIDNKPEYWYPQAKIIVKDLEDLHIVRLAIWPCHRINHQDMAERW 897

Query: 781 ARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSNTITIEISWN 816
            RR+VLVEEV+K+  ELDIQ+R  P+DIN+R++P+  +  +   W+
Sbjct: 898 TRRAVLVEEVIKILLELDIQHRFYPLDINVRTMPTVVSSRVPPGWS 903

BLAST of Cucsa.099120 vs. Swiss-Prot
Match: MSL5_ARATH (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana GN=MSL5 PE=2 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 4.2e-256
Identity = 460/834 (55.16%), Postives = 603/834 (72.30%), Query Frame = 1

Query: 1   MEDPPTKLIGDFLRKQKIEG-ETTLDMDLEMEELKPNRIVPPLAES--------PLSQTS 60
           + DPP+KLIG FL KQ+  G E +LD++L M EL+ N    P   S         +S++S
Sbjct: 83  LSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSNTPPRPATASNTPRRGLTTISESS 142

Query: 61  KDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQN 120
             +K   + D+          RR+NR S        GG S  +               +N
Sbjct: 143 SPVKTKVKADAVR--------RRQNRTS-------LGGSSDEEG--------------RN 202

Query: 121 ESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPA-------EPDRLSGLIPKS 180
              AE ++C S           +K  L R KTKSRL DPP        + +  SG   +S
Sbjct: 203 RDEAEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTHPAIDKTEMKSGR--RS 262

Query: 181 GQLRSGFLGKI------------EDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIIT 240
           G  +SGFLGK             E++++DPFL++DLP+EFKR   S    L+W+SL++I 
Sbjct: 263 GIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFWVFLEWISLVLIV 322

Query: 241 AALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRV 300
            +L+C+L++  L+ K+ W+LD+WKWEV + +LICGRLVS W +RI VF +E+NF  RKRV
Sbjct: 323 TSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRV 382

Query: 301 LYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLV 360
           LYFVYGVRK VQNCLWLGLVL+AWH LF+K+VE++T ++ L YV+RVLVCLL++ +IWLV
Sbjct: 383 LYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVTRVLVCLLVALIIWLV 442

Query: 361 KTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQ 420
           KT++VKVLASSFH+STYFDRIQESLF QYVIETLSGPPL+EI++ EEEE+++A++V+ L+
Sbjct: 443 KTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLE 502

Query: 421 N-AGIIIPPDLKAATFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDG--ITIDHL 480
             AG  +PP LKA    ++KS  ++G          KS  L+R  +K   DG  I ID L
Sbjct: 503 KLAGAKLPPALKA----TVKSFMKVG----------KSPGLNRIGSKRGEDGEGIRIDQL 562

Query: 481 HKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQ 540
            +++TKNVSAWNMKRL+NI+  G+ISTLD+ ++    +DE  T I+SE EAK AA+KIF 
Sbjct: 563 KRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSEYEAKCAARKIFH 622

Query: 541 NVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALA 600
           NV   G +YIYL+D +RF+ E+E  + M+LFEGA+E+ +ISKS LKNWVV AFRERRALA
Sbjct: 623 NVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVKAFRERRALA 682

Query: 601 LTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCK 660
           LTLNDTKTAVD+LH ++NV+ GI+I+I+WL++LGIA+++F + LSSQ+++VAF+FGN+CK
Sbjct: 683 LTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVFGNSCK 742

Query: 661 TIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLI 720
           TIFEAIIFLFVMHPFDVGDRCEIDG+Q+VVEEMNILTTVFLRYDN K+I PNSVL TK I
Sbjct: 743 TIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPI 802

Query: 721 HNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNK 780
            N+YRSPDMG+++EF VHIATP EKITA+K RI+SY++  K++WYPAPMIVF  +D LN 
Sbjct: 803 ANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNS 860

Query: 781 VKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLP 804
           VK+AVWL+HRMNHQD GER+ RR +L+EEV K C+ELDI+YRL P++IN+RSLP
Sbjct: 863 VKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINVRSLP 860

BLAST of Cucsa.099120 vs. Swiss-Prot
Match: MSL4_ARATH (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana GN=MSL4 PE=3 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 1.8e-251
Identity = 446/837 (53.29%), Postives = 608/837 (72.64%), Query Frame = 1

Query: 3   DPPTKLIGDFLRKQKIEG-ETTLDMDLEMEELKPNRIVPPLAESPLSQTSKDLKVSFQHD 62
           DPP+KLI  FL KQK  G E +LDM+  M EL+ N  VPPL+ + +S ++  +       
Sbjct: 68  DPPSKLINQFLNKQKASGDEISLDMEANMPELQKNT-VPPLSSTAVSGSASPVTAPVTA- 127

Query: 63  STEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQNESLAEAMRCA 122
           S    + D   RR+NR +  L    K G S   + +        + G      +E ++C 
Sbjct: 128 SYRNGTGDAIRRRQNRVT--LSPSVKDGDSSEDEENR-------VDG------SEVVKCT 187

Query: 123 SNLSFHSELSFQRKSNLLRAKTKSRLIDPPAE--PDRLSGLIPKSGQLRSGFLG------ 182
           SN S        R   L++ KT+SRL+DPP    PD +SG  P+SG L  GF G      
Sbjct: 188 SNRST------MRTKTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGNLNPGFSGRNTKPG 247

Query: 183 -------KIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLRE 242
                  K  ++++DPF E+DLP+  ++       +++W+ LI+I A+LIC+L +PYLR 
Sbjct: 248 TPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASLICSLVIPYLRG 307

Query: 243 KSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNC 302
           K+LW+L +WKWEVM+ +LICGRLVS W +++ V+F+E NFL RK+VLYFVYG+RKPVQNC
Sbjct: 308 KTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNC 367

Query: 303 LWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHV 362
           LWLGLVLIAWH LF+K+VE++  +++L YV++VL+CLL++ +IWL+KTL+VKVLASSFH+
Sbjct: 368 LWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFHM 427

Query: 363 STYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAAT 422
           STYFDRIQESLF QYVIETLSGPP +EI     EEE++A++V+  +  G  + P    A 
Sbjct: 428 STYFDRIQESLFTQYVIETLSGPPRIEIHI---EEEKVANDVKTFEIVGRKLSPLGPKAV 487

Query: 423 FASIKSGREIGSGRTHKS--FCAKSCKLSRALTKNRN--DGITIDHLHKLSTKNVSAWNM 482
             S      +GSGR  KS     KS  LSR+ +K     +GI IDHL +++TKNVSAW M
Sbjct: 488 --SSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKM 547

Query: 483 KRLLNIVRYGSISTLDEQIRGPCL-DDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYL 542
           K+L+N+++ G++STLDEQI+     +D+  T+I+SE EAK AA+KIFQNVA  G +YIY+
Sbjct: 548 KKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYM 607

Query: 543 DDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDK 602
           +D +RF+ EDE  + M LFEGA+E  +ISKS LKNWVVNAFRERRALALTLNDTKTAV++
Sbjct: 608 EDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNR 667

Query: 603 LHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVM 662
           LH +V+V+  I+ILI+WL++LGIA++KF + +SSQ+++V F+FGN+CKTIFEA+IF+FVM
Sbjct: 668 LHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVM 727

Query: 663 HPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGES 722
           HPFDVGDRCEIDG+QM+VEEMNILTTVFLR+DN K++ PNS+L TK I N+YRSPDM ++
Sbjct: 728 HPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDA 787

Query: 723 IEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMN 782
           IEF VHIATP EK TA++ RI+SY++  K+HW+P+PMIVF+D+ GLN VK+A+W +H+MN
Sbjct: 788 IEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMN 847

Query: 783 HQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSNTIT---IEISWN 816
           HQ+ GER+ RR  L+EE+ ++C+ELDI+YRL P++IN++SLP++  IT   I  SWN
Sbjct: 848 HQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAATPITSDRIPPSWN 876

BLAST of Cucsa.099120 vs. Swiss-Prot
Match: MSL7_ARATH (Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana GN=MSL7 PE=3 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 7.3e-232
Identity = 397/688 (57.70%), Postives = 533/688 (77.47%), Query Frame = 1

Query: 134 RKSNLL-RAKTKSRLIDPPAEPDR-LSGLIPKSGQLRSGFLGKIEDD---DDDPFLEDDL 193
           RK+ L+ RAK +SRLIDPP E ++  S  I  S QLRSG LG+  DD   +DD   E+D+
Sbjct: 161 RKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSAEEDV 220

Query: 194 PDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGR 253
           P E+++    A+T+LQW+SLI +  AL+ +L +   R  +LW L +WKWEV++ +LICGR
Sbjct: 221 PVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVLICGR 280

Query: 254 LVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQT 313
           LVSG GIRI VFFIERNFLLRKRVLYFVYGV+  VQNCLWLGLVL+AWH LF+K+VEK+T
Sbjct: 281 LVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKVEKET 340

Query: 314 NTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSG 373
            + +L  +S++LVC L+ST++WL+KTL+VKVLASSFHVSTYFDRIQE+LF+ Y+IETLSG
Sbjct: 341 QSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIETLSG 400

Query: 374 PPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASIKSGREIGSGRTHKSFCAK 433
           PP++E+ + EEEE+R  DE+ K+Q  G  + P+L +A F   KSG  +            
Sbjct: 401 PPMLELSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSGSTM------------ 460

Query: 434 SCKLSRALTKNRND-GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLD 493
           + K S  + K  +D GIT+D LHK++ KNVSAWNMKRL+ IVR  S+STLDEQ      +
Sbjct: 461 NMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCE 520

Query: 494 DESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQ 553
           DEST +I+SE+EAKAAA+KIF+NVA  G K+IYL+DL+RF+R DE +KTM LFEGA   +
Sbjct: 521 DESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTK 580

Query: 554 RISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASS 613
           +I+KSALKNW+VNAFRERRALALTLNDTKTAV+KLHHM++ +  I+I+++WLI+L IA+S
Sbjct: 581 KITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATS 640

Query: 614 KFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTT 673
           K+ +FL+SQ+V++AF+FGN+ KT+FE+IIFLF++HP+DVGDR  ID ++MVVEEMNILTT
Sbjct: 641 KYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTT 700

Query: 674 VFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIE 733
           VFLR DNLK++ PN +L  K IHN+ RSPDMG+ +   VHI TP EKI A+K RI SYI+
Sbjct: 701 VFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYID 760

Query: 734 GNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELD 793
              E+WYP   ++ KD++ LN V++A+WL H++NHQ+ GER+ RR++L+EEV+K+  ELD
Sbjct: 761 SKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKILLELD 820

Query: 794 IQYRLLPIDINIRSLPSSNTITIEISWN 816
           IQYR  P+DIN++++P+  +  +  +W+
Sbjct: 821 IQYRFHPLDINVKTMPTVVSSRVPPAWS 836

BLAST of Cucsa.099120 vs. TrEMBL
Match: A0A0A0K619_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G017150 PE=4 SV=1)

HSP 1 Score: 1592.4 bits (4122), Expect = 0.0e+00
Identity = 807/817 (98.78%), Postives = 809/817 (99.02%), Query Frame = 1

Query: 1   MEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRIVPPLAESPLSQTSKDLKVSFQH 60
           MEDPPTKLIGDFL KQKIEGETTLDMDLEMEELKPNRIVPPLAESPLSQTSKDLKVSFQH
Sbjct: 136 MEDPPTKLIGDFLCKQKIEGETTLDMDLEMEELKPNRIVPPLAESPLSQTSKDLKVSFQH 195

Query: 61  DSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQNESLAEAMRC 120
           DSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQNESLAEAMRC
Sbjct: 196 DSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQNESLAEAMRC 255

Query: 121 ASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSGQLRSGFLGKIEDDDD 180
           ASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSGQLRSGFLGKIEDDDD
Sbjct: 256 ASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSGQLRSGFLGKIEDDDD 315

Query: 181 DPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVM 240
           DPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVM
Sbjct: 316 DPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVM 375

Query: 241 IFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLF 300
           IFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLF
Sbjct: 376 IFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLF 435

Query: 301 NKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQ 360
           NKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQ
Sbjct: 436 NKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQ 495

Query: 361 YVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASIKSGREIGSGR 420
           YVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASIKSGREIGSGR
Sbjct: 496 YVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASIKSGREIGSGR 555

Query: 421 THKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQ 480
           THKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQ
Sbjct: 556 THKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQ 615

Query: 481 IRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLF 540
           IRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLF
Sbjct: 616 IRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLF 675

Query: 541 EGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLI 600
           EGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLI
Sbjct: 676 EGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLI 735

Query: 601 VLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVE 660
           VLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVE
Sbjct: 736 VLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVE 795

Query: 661 EMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKH 720
           EMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKH
Sbjct: 796 EMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKH 855

Query: 721 RIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVV 780
           RIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVV
Sbjct: 856 RIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVV 915

Query: 781 KVCQELDIQYRLLPIDINIRSLPSS-NTITIEISWNS 817
           KVCQELDIQYRLLPIDINIRSLPSS  +I    +W S
Sbjct: 916 KVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTS 952

BLAST of Cucsa.099120 vs. TrEMBL
Match: A0A0A0KE62_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G124170 PE=4 SV=1)

HSP 1 Score: 1121.3 bits (2899), Expect = 0.0e+00
Identity = 557/814 (68.43%), Postives = 681/814 (83.66%), Query Frame = 1

Query: 1   MEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRI-VPPLAESPLSQTSKDLKVSFQ 60
           +EDPP+KLIG FL KQK  GE +LDMD+EM EL  ++  +  +AESP+ ++S++LKVSF+
Sbjct: 116 VEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFE 175

Query: 61  HDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQNESLAEAMR 120
             S EIS ND S+RRR+RDS  L EE +G Q PRQ            S  +++  AE +R
Sbjct: 176 SIS-EISEND-SMRRRHRDS-PLDEEHRG-QQPRQCDRRAHG-----SNGEDDGAAEVLR 235

Query: 121 CASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSGQLRSGFLGK-IEDD 180
           C+SN  F  ++SFQRKS+LLRAKTKSRL+DPP   DR SG +PKSGQ+RSG + K ++++
Sbjct: 236 CSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEE 295

Query: 181 DDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWE 240
           DDDPFLE+DLPDE+K+ N   LT+LQW SLI+I AAL+CTL++ Y R K LW+L++WKWE
Sbjct: 296 DDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWE 355

Query: 241 VMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHL 300
           VMI +LICGRLVSGWGIR+ VFFIERNFLLRKRVLYFVYGVRK VQNCLWLGLVLIAW+ 
Sbjct: 356 VMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNF 415

Query: 301 LFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLF 360
           LF+ +V+++  ++ L YV++VLVCLL+STL+WLVKTLMVKVLASSFHVSTYFDRIQ++LF
Sbjct: 416 LFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALF 475

Query: 361 NQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASI-KSGREIG 420
           NQYVIETLSGPPL+EI+KNEEEEER+A+EV KLQNAG  IPPDLKA  F++  K GR IG
Sbjct: 476 NQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIG 535

Query: 421 SGRTHKSFCAKSCKLSRALTKNRND-GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSIST 480
           SG   KS   +S KLSR L+K   D GITIDHLHKLS KNVSAWNMKRL+NIVR+G++ST
Sbjct: 536 SGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLST 595

Query: 481 LDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKT 540
           LDEQI+    +DESTTEIKSE EAK AAKKIF NVA  G KYIYL+DL+RFM +DE  KT
Sbjct: 596 LDEQIKDTAHEDESTTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKT 655

Query: 541 MSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILI 600
           M LFEGA E+++ISKS+LKNWVVNAFRERRALALTLNDTKTAV+KLH MVN++  ++IL+
Sbjct: 656 MGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILV 715

Query: 601 LWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQ 660
           +WL++LGIA+SKF +F++SQ+V+VAF+FGNTCKT+FE+IIFLFVMHPFDVGDRCEIDG+Q
Sbjct: 716 IWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQ 775

Query: 661 MVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKIT 720
           M+VEEMNILTT+FLRYDN K+I PNSVLATK IHNFYRSPDMG+ IEF +HI+TP EKI 
Sbjct: 776 MIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIA 835

Query: 721 AMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLV 780
            M+ RIISYIEG KEHW PAPMIV KD++ LN++++A+WL+HRMNHQD GERW RR++LV
Sbjct: 836 IMRQRIISYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLV 895

Query: 781 EEVVKVCQELDIQYRLLPIDINIRSLPSSNTITI 811
           EE+VK+ QELD+QYRLLP+DIN+RSLP  N+  +
Sbjct: 896 EELVKIFQELDLQYRLLPLDINVRSLPPVNSTNL 920

BLAST of Cucsa.099120 vs. TrEMBL
Match: K7LLZ5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_10G284300 PE=4 SV=1)

HSP 1 Score: 1068.1 bits (2761), Expect = 5.1e-309
Identity = 543/830 (65.42%), Postives = 671/830 (80.84%), Query Frame = 1

Query: 2   EDPPTKLIGDFLRKQKIEGETTLDMDLEMEELK-------PNRIVPPLAESPLS-QTSKD 61
           EDPP++LIG FL KQ+  GE  LDMDLEMEEL+        +  + P+ ESP++ + S++
Sbjct: 105 EDPPSQLIGRFLHKQRASGEMQLDMDLEMEELQREGGDDDDDGKLTPVEESPMTHRVSRE 164

Query: 62  LKVSFQHDSTEIS----SNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGV 121
           LKVSF+  +  ++     ND   RR ++DS  L E     Q P Q P ++R  SP+ S  
Sbjct: 165 LKVSFEEPAYNVNFLETQNDAVRRRHSKDSPSLAEF----QRPPQPPQYDRRRSPSPSPA 224

Query: 122 QNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSGQLRS 181
             +   E +RC SN SF   LS QRKS LL+AKT+SRL+DPP EPDR SG + KSGQL S
Sbjct: 225 CGD---EVVRCTSNASFERNLSMQRKSALLKAKTRSRLMDPPEEPDRKSGRVLKSGQLLS 284

Query: 182 GFLGKIEDD-DDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKS 241
           GFLGK  D+ DDDPFLE+DLPDEFK  +FS   +L+W+SLI I   LI TL VP+LR K+
Sbjct: 285 GFLGKKNDEEDDDPFLEEDLPDEFKETHFSLWILLEWLSLISIIGLLITTLCVPFLRNKN 344

Query: 242 LWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLW 301
           LW+L +WKWEVM+ +LICGRLVS W +RI VF IERNFLLRKRVLYFVYGVRK VQNC+W
Sbjct: 345 LWQLRLWKWEVMVLVLICGRLVSDWVVRIAVFCIERNFLLRKRVLYFVYGVRKAVQNCVW 404

Query: 302 LGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVST 361
           LGLVLIAWHLLF+KRV+++T++  L YV++VLVC L+ TL+WL+KTLMVKVLASSFHVST
Sbjct: 405 LGLVLIAWHLLFDKRVQRETHSDFLEYVTKVLVCFLVGTLVWLLKTLMVKVLASSFHVST 464

Query: 362 YFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFA 421
           YFDRIQESLFNQ+VIETLSGPPLVEI+K EEEEER+ADEVQKLQNAG+ IPPDL+A+ F+
Sbjct: 465 YFDRIQESLFNQFVIETLSGPPLVEIQKAEEEEERLADEVQKLQNAGVTIPPDLRASAFS 524

Query: 422 SIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDG--ITIDHLHKLSTKNVSAWNMKRLL 481
           +IKSGR + SG   KS   KS K SR L+K  ++G  IT+D+LHKL+  N+SAWNMKRL+
Sbjct: 525 NIKSGR-LRSGMLQKSPRVKSGKFSRPLSKKSDEGNVITMDNLHKLNPNNISAWNMKRLM 584

Query: 482 NIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVR 541
           N+VR+G++STLDEQI     DD++ T+I+SE EAKAAAKKIF NVA RG +YIY DDL+R
Sbjct: 585 NMVRHGALSTLDEQILDNSNDDDNATQIRSEYEAKAAAKKIFHNVARRGCRYIYPDDLMR 644

Query: 542 FMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMV 601
           FMREDE  KTM+LFEGA+E  +ISKSALKNWVVNAFRERRALALTLNDTKTAV+KLH M+
Sbjct: 645 FMREDEAAKTMNLFEGASEAGKISKSALKNWVVNAFRERRALALTLNDTKTAVNKLHRML 704

Query: 602 NVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDV 661
           N I GI+IL++WL++L +A++KF +F+SSQ+VVVAFIFGNTCKTIFEAIIFLFVMHPFDV
Sbjct: 705 NFIVGIIILVIWLLILELATTKFLLFVSSQVVVVAFIFGNTCKTIFEAIIFLFVMHPFDV 764

Query: 662 GDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLV 721
           GDRCEIDG+QMVVEEMNILTT+FLR+DN KVIIPN+VLATK I+N+YRSPDMG++IEF V
Sbjct: 765 GDRCEIDGVQMVVEEMNILTTIFLRFDNQKVIIPNNVLATKAIYNYYRSPDMGDAIEFCV 824

Query: 722 HIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSG 781
           HI+TP EKI+ +KHRI SYI+  KEHWYP+P+IV++D D LN V+LA+W +HRMN QD G
Sbjct: 825 HISTPVEKISLIKHRIQSYIDNKKEHWYPSPLIVYRDYDQLNMVRLAIWPTHRMNFQDMG 884

Query: 782 ERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSNTITIEISWNS 817
           ER+ RRS+L+EE++K+ +ELDI YRLLP+DIN+R+ P+++   +  SW S
Sbjct: 885 ERFVRRSLLLEEMIKIFRELDINYRLLPMDINVRATPTTSD-RLPPSWTS 925

BLAST of Cucsa.099120 vs. TrEMBL
Match: W9QBV6_9ROSA (Mechanosensitive ion channel protein 8 OS=Morus notabilis GN=L484_006078 PE=4 SV=1)

HSP 1 Score: 1067.0 bits (2758), Expect = 1.1e-308
Identity = 544/826 (65.86%), Postives = 665/826 (80.51%), Query Frame = 1

Query: 2   EDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNR------IVPPLAESPL-SQTSKDL 61
           EDPP+KLI  FL +QK  G+ +LDMDLEMEEL+ +        + P++ESP  S++S++L
Sbjct: 127 EDPPSKLIRQFLHRQKASGDISLDMDLEMEELRRSAGSGGELDLTPVSESPQNSRSSREL 186

Query: 62  KVSFQHD----STEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQ 121
           KVS         +EIS ND ++RRR +DS +  +ES            ER G        
Sbjct: 187 KVSLHPSPSDVGSEISQND-AVRRRFKDSPNDNDESL--------ERRERDGE------- 246

Query: 122 NESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSGQL--- 181
                E +RC SN SF  E+SFQR+S+ LR KT+SRL DPP EPD  SG IPKSGQ+   
Sbjct: 247 -----EVVRCTSNASFQREISFQRRSSFLRVKTRSRLQDPPEEPDMRSGRIPKSGQIPRS 306

Query: 182 ---------RSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICT 241
                    +SG LGK ++++DDPF ++DLPDEFK+ N SALT+LQW SLI+I     CT
Sbjct: 307 GPIPRSGPMKSGLLGK-DEEEDDPFSDEDLPDEFKKANLSALTLLQWASLILIVGLFACT 366

Query: 242 LSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYG 301
           LS+ YLR K+L +L +WKWEV+I +LICGRLVSGWGIRI VFF ERNFLLRKRVLYFVYG
Sbjct: 367 LSIHYLRHKNLLKLKLWKWEVLILVLICGRLVSGWGIRIAVFFFERNFLLRKRVLYFVYG 426

Query: 302 VRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVK 361
           VRK VQNCLWLGLVLIAWH LF+KRVE++T +  L YV++VL+C L+ TL+WLVKTL+VK
Sbjct: 427 VRKAVQNCLWLGLVLIAWHFLFDKRVERETRSESLKYVTKVLICFLVGTLLWLVKTLIVK 486

Query: 362 VLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIII 421
           VLASSFHVSTYFDRIQ+SLFNQ+VIETLSGPPL+EI K EEEEER+ADEV+KLQNAG  I
Sbjct: 487 VLASSFHVSTYFDRIQDSLFNQFVIETLSGPPLIEIYKTEEEEERLADEVEKLQNAGATI 546

Query: 422 PPDLKAATFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVS 481
           PPDL A + A  ++ R IGSGR  +++  KS KLSR+L+K  ++GITIDHLHKL+ KNVS
Sbjct: 547 PPDL-AFSSALPRTSRVIGSGRLSRTW--KSSKLSRSLSKIGDEGITIDHLHKLNPKNVS 606

Query: 482 AWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKY 541
           AWNMKRL+ +VR+G+++TLDEQI     +DES T+I+SE EAKAAAKKIFQNVA RG K+
Sbjct: 607 AWNMKRLMRMVRHGTLTTLDEQIVDSTHEDESATQIRSEVEAKAAAKKIFQNVARRGSKF 666

Query: 542 IYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTA 601
           I L+DL+RFMREDE +KTMSLFEGA+E+QRISKS+LKNWVVNAFRERRALALTLNDTKTA
Sbjct: 667 ICLEDLMRFMREDEAVKTMSLFEGASESQRISKSSLKNWVVNAFRERRALALTLNDTKTA 726

Query: 602 VDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFL 661
           V+KLH +VNVI  I+I ++WL++LGIA+SKF +F+SSQ+V+VAFIFGNTCKT+FEAI+FL
Sbjct: 727 VNKLHRIVNVIVAIVIGVIWLLILGIATSKFLLFISSQLVLVAFIFGNTCKTVFEAIVFL 786

Query: 662 FVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDM 721
           FVMHPFDVGDRCEIDG+QMVVEEMNILTTVFLRYDN K+I PNSVLATK I NFYRSPDM
Sbjct: 787 FVMHPFDVGDRCEIDGVQMVVEEMNILTTVFLRYDNAKIIFPNSVLATKAIDNFYRSPDM 846

Query: 722 GESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSH 781
           G+ IEF +H+ATPA+KI AM+ RI +Y+E  KEHWYPAPM++ KD+D LN+V++AVWL H
Sbjct: 847 GDGIEFSIHVATPADKIAAMRQRITNYVENKKEHWYPAPMVIMKDLDELNRVRMAVWLCH 906

Query: 782 RMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPS 805
           R+NHQD GER+ARRS+L+EE+VK+ QELDIQYRLLPIDIN+R++PS
Sbjct: 907 RINHQDMGERYARRSLLIEEMVKIFQELDIQYRLLPIDINVRAMPS 927

BLAST of Cucsa.099120 vs. TrEMBL
Match: A0A0R0ELK1_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_20G105400 PE=4 SV=1)

HSP 1 Score: 1064.3 bits (2751), Expect = 7.4e-308
Identity = 540/826 (65.38%), Postives = 669/826 (80.99%), Query Frame = 1

Query: 2   EDPPTKLIGDFLRKQKIEGETTLDMDLEMEELK---PNRIVPPLAESPLS-QTSKDLKVS 61
           EDPP++LIG FL KQ+  GE  LDMDLEMEEL+    +  + P+ ESP++ + S++LKVS
Sbjct: 126 EDPPSQLIGHFLHKQRASGEMQLDMDLEMEELQREGDDGKLTPVDESPVTHRVSRELKVS 185

Query: 62  FQHDSTEIS----SNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQNES 121
           F+  +  ++     ND   RR ++DS  + E     Q P Q P H+   SP+ S   +E 
Sbjct: 186 FEEPTCNVNFLEAQNDAVRRRHSKDSPSIAEF----QRPPQPPQHDCRRSPSPSPAGDE- 245

Query: 122 LAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSGQLRSGFLG 181
             E +RC SN SF   LS QRKS LL+AKT+SRL+DPP EPDR S  + KS QL SGFLG
Sbjct: 246 --EVVRCTSNASFERSLSMQRKSALLKAKTRSRLMDPPEEPDRKSSRVLKSSQLLSGFLG 305

Query: 182 KIEDDDD-DPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWEL 241
           K  D++D DPFLE+DLPDEFK  +FS   +L+W+SLI+I   LI TL VP+LR K LW+L
Sbjct: 306 KKNDEEDEDPFLEEDLPDEFKETHFSLWILLEWLSLILIIGLLITTLCVPFLRNKDLWQL 365

Query: 242 DIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLV 301
            +WKWEVM+ +LICGRLVS W IRI VF IERNFLLRKRVLYFVYGV+K VQNC+WLGLV
Sbjct: 366 RLWKWEVMVLVLICGRLVSDWVIRIAVFCIERNFLLRKRVLYFVYGVKKAVQNCVWLGLV 425

Query: 302 LIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDR 361
           LIAWHLLF+KRV+++T ++ L YV++VLVC L+ TL+WL+KTLMVKVLASSFHVSTYFDR
Sbjct: 426 LIAWHLLFDKRVQRETRSNFLEYVTKVLVCFLVGTLVWLLKTLMVKVLASSFHVSTYFDR 485

Query: 362 IQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASIKS 421
           IQESLFNQ+VIETLSGPPLVEIRK EEEEER+ADEVQKLQNAG+ IPPDL+A+ F++IKS
Sbjct: 486 IQESLFNQFVIETLSGPPLVEIRKAEEEEERLADEVQKLQNAGVTIPPDLRASAFSNIKS 545

Query: 422 GREIGSGRTHKSFCAKSCKLSRALTKNRNDG--ITIDHLHKLSTKNVSAWNMKRLLNIVR 481
           GR + SG   KS   KS K SR L+K  ++   IT+D+LHKL+  N+SAWNMKRL+N+VR
Sbjct: 546 GR-LRSGMLPKSPRFKSDKFSRPLSKKSDEPNMITMDNLHKLNPNNISAWNMKRLMNMVR 605

Query: 482 YGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMRE 541
            G++STLDEQI    +DDE+ T+I+SE EAKAAAKKIFQNVA RG +YIY DDL+RFMRE
Sbjct: 606 NGALSTLDEQILDNSMDDENATQIRSENEAKAAAKKIFQNVARRGCRYIYPDDLMRFMRE 665

Query: 542 DEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIF 601
           DE  KTM+LFEGA+E +RISKSALKNWVVNAFRERRALALTLNDTKTAV+KLH M+N I 
Sbjct: 666 DEAAKTMNLFEGASEAERISKSALKNWVVNAFRERRALALTLNDTKTAVNKLHRMLNFIV 725

Query: 602 GILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRC 661
            I+IL++WL++L +A++KF +F+SSQ+VVVAF+FGNTCKTIFEAIIFLFVMHPFDVGDRC
Sbjct: 726 AIVILVIWLLILELATTKFLLFVSSQVVVVAFVFGNTCKTIFEAIIFLFVMHPFDVGDRC 785

Query: 662 EIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIAT 721
           EIDG+QMVVEEMNILTT+FLRYDN KVIIPN+VLATK I+N+YRSPDMG++IEF +HI+T
Sbjct: 786 EIDGVQMVVEEMNILTTIFLRYDNQKVIIPNNVLATKAIYNYYRSPDMGDAIEFCLHIST 845

Query: 722 PAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWA 781
           P EKI+ +KHRI SYI+  KEHWYP+P+IV++D D LN V++A+W +HRMN QD GER+ 
Sbjct: 846 PVEKISLIKHRIQSYIDNKKEHWYPSPLIVYRDYDQLNMVRMAIWPTHRMNFQDMGERFV 905

Query: 782 RRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSNTITIEISWNS 817
           RRS+L+EE++K+ +ELDI YRLLP+DIN+R+ P+++   +  SW S
Sbjct: 906 RRSLLLEEMIKIFRELDINYRLLPLDINVRATPTTSD-RLPPSWAS 942

BLAST of Cucsa.099120 vs. TAIR10
Match: AT1G78610.1 (AT1G78610.1 mechanosensitive channel of small conductance-like 6)

HSP 1 Score: 906.7 bits (2342), Expect = 1.0e-263
Identity = 466/809 (57.60%), Postives = 586/809 (72.44%), Query Frame = 1

Query: 2   EDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRIVPPLAESPLSQTSKDLKVSFQHD 61
           EDPPTKLIG FL KQ+  GE  LDMDL M+EL+ +R + P++ESP   T +D        
Sbjct: 82  EDPPTKLIGQFLHKQQASGEICLDMDLGMDELQ-SRGLTPVSESPRVSTKRD-------- 141

Query: 62  STEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQNESLAEAMRCA 121
              +   D      N D  ++ + S G  +P Q               ++ S    MR  
Sbjct: 142 --PVGRRDSRSNTNNNDDGEVVKCS-GNNAPIQ---------------RSSSTLLKMRTR 201

Query: 122 SNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSGQLRSGFLGKIEDDDDD 181
           S LS         ++    A  KS  I  P      SG   KS + +       E+++DD
Sbjct: 202 SRLSDPPTPQLPPQT----ADMKSGRI--PKSGQMKSGFFGKSPKTQG------EEEEDD 261

Query: 182 PFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMI 241
           PF  +DLP+E+++   S   VL+W+SLI+I A  +CTL++P LR+K LWEL +WKWE M+
Sbjct: 262 PFAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMV 321

Query: 242 FILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFN 301
            +LICGRLVS W ++I VFFIERNFLLRKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+
Sbjct: 322 LVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFD 381

Query: 302 KRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQY 361
           ++V K  NT  L  V+++ VCLL+  L+WLVKTL+VKVLASSFH+STYFDRIQESLF QY
Sbjct: 382 EKVAKAANTKALRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQY 441

Query: 362 VIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASIKSGREIGSGRT 421
           VIETLSGPPL+EI+KNEEEEERI+ EV+K QN G +            I+SG +    +T
Sbjct: 442 VIETLSGPPLIEIQKNEEEEERISVEVKKFQNPGGV-----------EIQSGAQKSPMKT 501

Query: 422 HKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQI 481
            KS        +       N GITID LHKL+ KNVSAW MKRL+NI+R GS++TLDEQ+
Sbjct: 502 GKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQL 561

Query: 482 RGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFE 541
           + P LDD+   +I+SE EAK AA+KIF NVA  G K+IY +D++RF+ +DE LKT+SLFE
Sbjct: 562 QDPSLDDDKGNQIRSEFEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFE 621

Query: 542 GAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIV 601
           GA+E  RISKS+LKNWVVNAFRERRALALTLNDTKTAV++LH MVN++ GI+IL++WLI+
Sbjct: 622 GASETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLII 681

Query: 602 LGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEE 661
           LGI S+KF + +SSQ+VVVAFIFGN CK +FE+II+LFV+HPFDVGDRCEIDG+QMVVEE
Sbjct: 682 LGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEE 741

Query: 662 MNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHR 721
           MNILTTVFLR+DN KV+ PNS+L TK I N+YRSPDMG+ IEF +HI TPAEKI  +K R
Sbjct: 742 MNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQR 801

Query: 722 IISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVK 781
           I SYIEG K+HWYPAPMIVFKD++ LN V++AVW +HRMNHQD GE+WARRS LVEE+ K
Sbjct: 802 ITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAK 840

Query: 782 VCQELDIQYRLLPIDINIRSLPSSNTITI 811
           +C+ELDI+YRL P+DIN+R+LP+S  + +
Sbjct: 862 ICRELDIEYRLYPLDINVRNLPTSTALPV 840

BLAST of Cucsa.099120 vs. TAIR10
Match: AT3G14810.1 (AT3G14810.1 mechanosensitive channel of small conductance-like 5)

HSP 1 Score: 885.6 bits (2287), Expect = 2.4e-257
Identity = 460/834 (55.16%), Postives = 603/834 (72.30%), Query Frame = 1

Query: 1   MEDPPTKLIGDFLRKQKIEG-ETTLDMDLEMEELKPNRIVPPLAES--------PLSQTS 60
           + DPP+KLIG FL KQ+  G E +LD++L M EL+ N    P   S         +S++S
Sbjct: 83  LSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSNTPPRPATASNTPRRGLTTISESS 142

Query: 61  KDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQN 120
             +K   + D+          RR+NR S        GG S  +               +N
Sbjct: 143 SPVKTKVKADAVR--------RRQNRTS-------LGGSSDEEG--------------RN 202

Query: 121 ESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPA-------EPDRLSGLIPKS 180
              AE ++C S           +K  L R KTKSRL DPP        + +  SG   +S
Sbjct: 203 RDEAEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTHPAIDKTEMKSGR--RS 262

Query: 181 GQLRSGFLGKI------------EDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIIT 240
           G  +SGFLGK             E++++DPFL++DLP+EFKR   S    L+W+SL++I 
Sbjct: 263 GIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFWVFLEWISLVLIV 322

Query: 241 AALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRV 300
            +L+C+L++  L+ K+ W+LD+WKWEV + +LICGRLVS W +RI VF +E+NF  RKRV
Sbjct: 323 TSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRV 382

Query: 301 LYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLV 360
           LYFVYGVRK VQNCLWLGLVL+AWH LF+K+VE++T ++ L YV+RVLVCLL++ +IWLV
Sbjct: 383 LYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVTRVLVCLLVALIIWLV 442

Query: 361 KTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQ 420
           KT++VKVLASSFH+STYFDRIQESLF QYVIETLSGPPL+EI++ EEEE+++A++V+ L+
Sbjct: 443 KTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLE 502

Query: 421 N-AGIIIPPDLKAATFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDG--ITIDHL 480
             AG  +PP LKA    ++KS  ++G          KS  L+R  +K   DG  I ID L
Sbjct: 503 KLAGAKLPPALKA----TVKSFMKVG----------KSPGLNRIGSKRGEDGEGIRIDQL 562

Query: 481 HKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQ 540
            +++TKNVSAWNMKRL+NI+  G+ISTLD+ ++    +DE  T I+SE EAK AA+KIF 
Sbjct: 563 KRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSEYEAKCAARKIFH 622

Query: 541 NVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALA 600
           NV   G +YIYL+D +RF+ E+E  + M+LFEGA+E+ +ISKS LKNWVV AFRERRALA
Sbjct: 623 NVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVKAFRERRALA 682

Query: 601 LTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCK 660
           LTLNDTKTAVD+LH ++NV+ GI+I+I+WL++LGIA+++F + LSSQ+++VAF+FGN+CK
Sbjct: 683 LTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVFGNSCK 742

Query: 661 TIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLI 720
           TIFEAIIFLFVMHPFDVGDRCEIDG+Q+VVEEMNILTTVFLRYDN K+I PNSVL TK I
Sbjct: 743 TIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPI 802

Query: 721 HNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNK 780
            N+YRSPDMG+++EF VHIATP EKITA+K RI+SY++  K++WYPAPMIVF  +D LN 
Sbjct: 803 ANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNS 860

Query: 781 VKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLP 804
           VK+AVWL+HRMNHQD GER+ RR +L+EEV K C+ELDI+YRL P++IN+RSLP
Sbjct: 863 VKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINVRSLP 860

BLAST of Cucsa.099120 vs. TAIR10
Match: AT1G53470.1 (AT1G53470.1 mechanosensitive channel of small conductance-like 4)

HSP 1 Score: 870.2 bits (2247), Expect = 1.0e-252
Identity = 446/837 (53.29%), Postives = 608/837 (72.64%), Query Frame = 1

Query: 3   DPPTKLIGDFLRKQKIEG-ETTLDMDLEMEELKPNRIVPPLAESPLSQTSKDLKVSFQHD 62
           DPP+KLI  FL KQK  G E +LDM+  M EL+ N  VPPL+ + +S ++  +       
Sbjct: 68  DPPSKLINQFLNKQKASGDEISLDMEANMPELQKNT-VPPLSSTAVSGSASPVTAPVTA- 127

Query: 63  STEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQNESLAEAMRCA 122
           S    + D   RR+NR +  L    K G S   + +        + G      +E ++C 
Sbjct: 128 SYRNGTGDAIRRRQNRVT--LSPSVKDGDSSEDEENR-------VDG------SEVVKCT 187

Query: 123 SNLSFHSELSFQRKSNLLRAKTKSRLIDPPAE--PDRLSGLIPKSGQLRSGFLG------ 182
           SN S        R   L++ KT+SRL+DPP    PD +SG  P+SG L  GF G      
Sbjct: 188 SNRST------MRTKTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGNLNPGFSGRNTKPG 247

Query: 183 -------KIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLRE 242
                  K  ++++DPF E+DLP+  ++       +++W+ LI+I A+LIC+L +PYLR 
Sbjct: 248 TPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASLICSLVIPYLRG 307

Query: 243 KSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNC 302
           K+LW+L +WKWEVM+ +LICGRLVS W +++ V+F+E NFL RK+VLYFVYG+RKPVQNC
Sbjct: 308 KTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNC 367

Query: 303 LWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHV 362
           LWLGLVLIAWH LF+K+VE++  +++L YV++VL+CLL++ +IWL+KTL+VKVLASSFH+
Sbjct: 368 LWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFHM 427

Query: 363 STYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAAT 422
           STYFDRIQESLF QYVIETLSGPP +EI     EEE++A++V+  +  G  + P    A 
Sbjct: 428 STYFDRIQESLFTQYVIETLSGPPRIEIHI---EEEKVANDVKTFEIVGRKLSPLGPKAV 487

Query: 423 FASIKSGREIGSGRTHKS--FCAKSCKLSRALTKNRN--DGITIDHLHKLSTKNVSAWNM 482
             S      +GSGR  KS     KS  LSR+ +K     +GI IDHL +++TKNVSAW M
Sbjct: 488 --SSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKM 547

Query: 483 KRLLNIVRYGSISTLDEQIRGPCL-DDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYL 542
           K+L+N+++ G++STLDEQI+     +D+  T+I+SE EAK AA+KIFQNVA  G +YIY+
Sbjct: 548 KKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYM 607

Query: 543 DDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDK 602
           +D +RF+ EDE  + M LFEGA+E  +ISKS LKNWVVNAFRERRALALTLNDTKTAV++
Sbjct: 608 EDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNR 667

Query: 603 LHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVM 662
           LH +V+V+  I+ILI+WL++LGIA++KF + +SSQ+++V F+FGN+CKTIFEA+IF+FVM
Sbjct: 668 LHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVM 727

Query: 663 HPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGES 722
           HPFDVGDRCEIDG+QM+VEEMNILTTVFLR+DN K++ PNS+L TK I N+YRSPDM ++
Sbjct: 728 HPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDA 787

Query: 723 IEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMN 782
           IEF VHIATP EK TA++ RI+SY++  K+HW+P+PMIVF+D+ GLN VK+A+W +H+MN
Sbjct: 788 IEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMN 847

Query: 783 HQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSNTIT---IEISWN 816
           HQ+ GER+ RR  L+EE+ ++C+ELDI+YRL P++IN++SLP++  IT   I  SWN
Sbjct: 848 HQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAATPITSDRIPPSWN 876

BLAST of Cucsa.099120 vs. TAIR10
Match: AT2G17000.1 (AT2G17000.1 Mechanosensitive ion channel family protein)

HSP 1 Score: 805.1 bits (2078), Expect = 4.1e-233
Identity = 397/688 (57.70%), Postives = 533/688 (77.47%), Query Frame = 1

Query: 134 RKSNLL-RAKTKSRLIDPPAEPDR-LSGLIPKSGQLRSGFLGKIEDD---DDDPFLEDDL 193
           RK+ L+ RAK +SRLIDPP E ++  S  I  S QLRSG LG+  DD   +DD   E+D+
Sbjct: 161 RKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSAEEDV 220

Query: 194 PDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGR 253
           P E+++    A+T+LQW+SLI +  AL+ +L +   R  +LW L +WKWEV++ +LICGR
Sbjct: 221 PVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVLICGR 280

Query: 254 LVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQT 313
           LVSG GIRI VFFIERNFLLRKRVLYFVYGV+  VQNCLWLGLVL+AWH LF+K+VEK+T
Sbjct: 281 LVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKVEKET 340

Query: 314 NTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSG 373
            + +L  +S++LVC L+ST++WL+KTL+VKVLASSFHVSTYFDRIQE+LF+ Y+IETLSG
Sbjct: 341 QSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIETLSG 400

Query: 374 PPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASIKSGREIGSGRTHKSFCAK 433
           PP++E+ + EEEE+R  DE+ K+Q  G  + P+L +A F   KSG  +            
Sbjct: 401 PPMLELSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSGSTM------------ 460

Query: 434 SCKLSRALTKNRND-GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLD 493
           + K S  + K  +D GIT+D LHK++ KNVSAWNMKRL+ IVR  S+STLDEQ      +
Sbjct: 461 NMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCE 520

Query: 494 DESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQ 553
           DEST +I+SE+EAKAAA+KIF+NVA  G K+IYL+DL+RF+R DE +KTM LFEGA   +
Sbjct: 521 DESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTK 580

Query: 554 RISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASS 613
           +I+KSALKNW+VNAFRERRALALTLNDTKTAV+KLHHM++ +  I+I+++WLI+L IA+S
Sbjct: 581 KITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATS 640

Query: 614 KFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTT 673
           K+ +FL+SQ+V++AF+FGN+ KT+FE+IIFLF++HP+DVGDR  ID ++MVVEEMNILTT
Sbjct: 641 KYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTT 700

Query: 674 VFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIE 733
           VFLR DNLK++ PN +L  K IHN+ RSPDMG+ +   VHI TP EKI A+K RI SYI+
Sbjct: 701 VFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYID 760

Query: 734 GNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELD 793
              E+WYP   ++ KD++ LN V++A+WL H++NHQ+ GER+ RR++L+EEV+K+  ELD
Sbjct: 761 SKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKILLELD 820

Query: 794 IQYRLLPIDINIRSLPSSNTITIEISWN 816
           IQYR  P+DIN++++P+  +  +  +W+
Sbjct: 821 IQYRFHPLDINVKTMPTVVSSRVPPAWS 836

BLAST of Cucsa.099120 vs. TAIR10
Match: AT5G12080.1 (AT5G12080.1 mechanosensitive channel of small conductance-like 10)

HSP 1 Score: 502.3 bits (1292), Expect = 5.7e-142
Identity = 262/631 (41.52%), Postives = 401/631 (63.55%), Query Frame = 1

Query: 175 IEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDI 234
           +E D+D+   +    +   R   S L +++    ++I +AL+ +L++  L+  + W L++
Sbjct: 142 VEKDEDEEIYKKVKLNREMRSKISTLALIESAFFVVILSALVASLTINVLKHHTFWGLEV 201

Query: 235 WKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLI 294
           WKW V++ ++  G LV+ W +R+ VF IE NFLLR++VLYFV+G++K VQ  +WL L+L+
Sbjct: 202 WKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIWLCLILV 261

Query: 295 AWHLLFNKRVEKQ-TNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRI 354
           AW LLFN  V++    T +L  ++R L+ +L     WLVKTL++K+LA++F+V+ +FDRI
Sbjct: 262 AWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVNNFFDRI 321

Query: 355 QESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFAS-IKS 414
           Q+S+F+QYV++TLSG PL+      EE ER+  E            P     +FA+ +K 
Sbjct: 322 QDSVFHQYVLQTLSGLPLM------EEAERVGRE------------PSTGHLSFATVVKK 381

Query: 415 GREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRLLNIVRYG 474
           G                       T      I +  +HK+  + VSAW M+ L+  VR  
Sbjct: 382 G-----------------------TVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTS 441

Query: 475 SIS----TLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFM 534
            +S    TLDE   G    +++  EI SE EA AAA  +F+NVA   + YI  +DL+RFM
Sbjct: 442 GLSTISDTLDETAYGEG-KEQADREITSEMEALAAAYHVFRNVAQPFFNYIEEEDLLRFM 501

Query: 535 REDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNV 594
            ++EV     LF+GAAE  RI++ A   WVV  +  RRALA +LNDTKTAV +L+ +V  
Sbjct: 502 IKEEVDLVFPLFDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTA 561

Query: 595 IFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGD 654
           I  ++ +++WL++L +A++K  +F S+Q+V +AFI G+TCK +FE+I+F+FVMHP+DVGD
Sbjct: 562 ILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGD 621

Query: 655 RCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHI 714
           RC +DG+ M+VEEMN+LTTVFL+ +N KV  PN+VLATK I N++RSP+MGE++EF +  
Sbjct: 622 RCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISF 681

Query: 715 ATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGER 774
           +TP  KI  +K RI  Y+E N +HW P   +V K+I+ +NK+K+A++  H +  Q++ ER
Sbjct: 682 STPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRER 730

Query: 775 WARRSVLVEEVVKVCQELDIQYRLLPIDINI 800
             RR+ L   + ++ ++L I Y LLP DIN+
Sbjct: 742 NLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of Cucsa.099120 vs. NCBI nr
Match: gi|449454364|ref|XP_004144925.1| (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis sativus])

HSP 1 Score: 1592.4 bits (4122), Expect = 0.0e+00
Identity = 807/817 (98.78%), Postives = 809/817 (99.02%), Query Frame = 1

Query: 1   MEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRIVPPLAESPLSQTSKDLKVSFQH 60
           MEDPPTKLIGDFL KQKIEGETTLDMDLEMEELKPNRIVPPLAESPLSQTSKDLKVSFQH
Sbjct: 136 MEDPPTKLIGDFLCKQKIEGETTLDMDLEMEELKPNRIVPPLAESPLSQTSKDLKVSFQH 195

Query: 61  DSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQNESLAEAMRC 120
           DSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQNESLAEAMRC
Sbjct: 196 DSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQNESLAEAMRC 255

Query: 121 ASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSGQLRSGFLGKIEDDDD 180
           ASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSGQLRSGFLGKIEDDDD
Sbjct: 256 ASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSGQLRSGFLGKIEDDDD 315

Query: 181 DPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVM 240
           DPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVM
Sbjct: 316 DPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVM 375

Query: 241 IFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLF 300
           IFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLF
Sbjct: 376 IFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLF 435

Query: 301 NKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQ 360
           NKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQ
Sbjct: 436 NKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQ 495

Query: 361 YVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASIKSGREIGSGR 420
           YVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASIKSGREIGSGR
Sbjct: 496 YVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASIKSGREIGSGR 555

Query: 421 THKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQ 480
           THKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQ
Sbjct: 556 THKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQ 615

Query: 481 IRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLF 540
           IRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLF
Sbjct: 616 IRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLF 675

Query: 541 EGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLI 600
           EGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLI
Sbjct: 676 EGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLI 735

Query: 601 VLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVE 660
           VLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVE
Sbjct: 736 VLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVE 795

Query: 661 EMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKH 720
           EMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKH
Sbjct: 796 EMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKH 855

Query: 721 RIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVV 780
           RIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVV
Sbjct: 856 RIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVV 915

Query: 781 KVCQELDIQYRLLPIDINIRSLPSS-NTITIEISWNS 817
           KVCQELDIQYRLLPIDINIRSLPSS  +I    +W S
Sbjct: 916 KVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTS 952

BLAST of Cucsa.099120 vs. NCBI nr
Match: gi|659094533|ref|XP_008448113.1| (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis melo])

HSP 1 Score: 1502.6 bits (3889), Expect = 0.0e+00
Identity = 764/817 (93.51%), Postives = 778/817 (95.23%), Query Frame = 1

Query: 1   MEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRIVPPLAESPLSQTSKDLKVSFQH 60
           MEDPPTKLIGDFL KQKI GETTLDMDLEMEELKPNRI+PPLAESPLSQTSKDLKVSFQ 
Sbjct: 133 MEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIPPLAESPLSQTSKDLKVSFQQ 192

Query: 61  DSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQNESLAEAMRC 120
           DSTEISSNDQS+RRR RDS+DLKEE KG Q P QQ HHERLGSPTISGVQNESLAEAMRC
Sbjct: 193 DSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLGSPTISGVQNESLAEAMRC 252

Query: 121 ASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSGQLRSGFLGKIEDDDD 180
           ASNLSFHSELSFQRKSNLLRAKTKSRL DPPAEPDRLSGLIPKSGQLRSGFLGKIEDDDD
Sbjct: 253 ASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGLIPKSGQLRSGFLGKIEDDDD 312

Query: 181 DPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVM 240
           DPFLEDDLPD+FKRGNF+ALTVLQW SLI+ITAA ICTLSVPYLREKSLWELDIWKWEVM
Sbjct: 313 DPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYLREKSLWELDIWKWEVM 372

Query: 241 IFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLF 300
           I ILICGRLVSGWGIRI VFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLF
Sbjct: 373 ILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLF 432

Query: 301 NKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQ 360
           NKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQ
Sbjct: 433 NKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQ 492

Query: 361 YVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASIKSGREIGSGR 420
           YVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAG+ IPPDLK ATFASIKSGR I S R
Sbjct: 493 YVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDLK-ATFASIKSGRAISSER 552

Query: 421 THKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQ 480
           THKSFCAKS K SRALTKN NDGITIDHLHKLS KNVSAWNMKRLLNIVRYGSISTLDEQ
Sbjct: 553 THKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAWNMKRLLNIVRYGSISTLDEQ 612

Query: 481 IRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLF 540
           IRGPCLDDESTTEIKSEREAKAAAKKIFQNVA RGYKYIYLDDL+RFMREDEVLKT SLF
Sbjct: 613 IRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLMRFMREDEVLKTTSLF 672

Query: 541 EGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLI 600
           EGAAEN+RISKS LKNWVVN FRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLI
Sbjct: 673 EGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLI 732

Query: 601 VLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVE 660
           VLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDG QMVVE
Sbjct: 733 VLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGTQMVVE 792

Query: 661 EMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKH 720
           EMNILTTVFLRYDNLK+IIPNSVLATKLIHNFYRSPDMGES+EF VHIATPAEKITAMK 
Sbjct: 793 EMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIATPAEKITAMKQ 852

Query: 721 RIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVV 780
           RIISYIEGNKEHW PAPMIVFKDIDGLNK+KLAVWLSHRMNHQDS ERWARRSVLVEEVV
Sbjct: 853 RIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERWARRSVLVEEVV 912

Query: 781 KVCQELDIQYRLLPIDINIRSLPSS-NTITIEISWNS 817
           KVCQELDIQYRLLPIDINIRSLPSS  +I    +W S
Sbjct: 913 KVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTS 948

BLAST of Cucsa.099120 vs. NCBI nr
Match: gi|659095000|ref|XP_008448342.1| (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis melo])

HSP 1 Score: 1123.2 bits (2904), Expect = 0.0e+00
Identity = 556/811 (68.56%), Postives = 681/811 (83.97%), Query Frame = 1

Query: 1   MEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRI-VPPLAESPLSQTSKDLKVSFQ 60
           +EDPP+KLIG FL KQK  GE +LDMD+EM EL  ++  +  +AESP+ ++S++LKVSF+
Sbjct: 116 VEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFE 175

Query: 61  HDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQNESLAEAMR 120
             S EIS ND S+RRR+RDS  L EE +G Q PRQ            S  +++  AE +R
Sbjct: 176 SIS-EISEND-SMRRRHRDS-PLDEEHRG-QQPRQCDRRAHG-----SNGEDDGAAEVLR 235

Query: 121 CASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSGQLRSGFLGKI-EDD 180
           C+SN SF  ++SFQRKS+LLRAKTKSRL+DPP   DR SG +PKSGQ+RSG + K+ +++
Sbjct: 236 CSSNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKVLDEE 295

Query: 181 DDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWE 240
           DDDPFLE+DLPDE+K+ N   LT+LQW SLI+I AAL+CTL++ Y R K LW+L++WKWE
Sbjct: 296 DDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWE 355

Query: 241 VMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHL 300
           VMI +LICGRLVSGWGIR+ VFFIERNFLLRKRVLYFVYGVRK VQNCLWLGLVLIAW+ 
Sbjct: 356 VMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNF 415

Query: 301 LFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLF 360
           LF+ +V+++  ++ L YV++VLVCLLISTL+WLVKTLMVKVLASSFHVSTYFDRIQ++LF
Sbjct: 416 LFDDKVQREVKSNALEYVTKVLVCLLISTLVWLVKTLMVKVLASSFHVSTYFDRIQDALF 475

Query: 361 NQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASI-KSGREIG 420
           NQYVIETLSGPPL+EI+KNEEEEER+A+EV KLQNAG  IPPDLKA  F++  K GR IG
Sbjct: 476 NQYVIETLSGPPLIEIQKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAQKGGRVIG 535

Query: 421 SGRTHKSFCAKSCKLSRALTKNRND-GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSIST 480
           SG   KS   +S KLSR L+K   D GITIDHLHKLS KNVSAWNMKRL+NIVR+G++ST
Sbjct: 536 SGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLST 595

Query: 481 LDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKT 540
           LDEQI+    +DESTT+IKSE EAK AAKKIF NVA  G KYIYL+DL+RFM +DE  KT
Sbjct: 596 LDEQIKDTAHEDESTTQIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKT 655

Query: 541 MSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILI 600
           M LFEGA E+++ISKS+LKNWVVNAFRERRALALTLNDTKTAV+KLH MVN++  ++IL+
Sbjct: 656 MGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILV 715

Query: 601 LWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQ 660
           +WL++LGIA+SKF +F++SQ+V+VAF+FGNTCKT+FE+IIFLFVMHPFDVGDRCEIDG+Q
Sbjct: 716 IWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQ 775

Query: 661 MVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKIT 720
           M+VEEMNILTT+FLRYDN K+I PNSVLATK IHNFYRSPDMG+ +EF +HI+TP EKI 
Sbjct: 776 MIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGVEFCLHISTPPEKIA 835

Query: 721 AMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLV 780
            M+ RI+SYIEG KEHW PAPMIV KD++ LN++++A+WL+HRMNHQD GERW RR++LV
Sbjct: 836 IMRQRIVSYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLV 895

Query: 781 EEVVKVCQELDIQYRLLPIDINIRSLPSSNT 808
           EE+VK+ QELD+QYRLLP+DIN+RSLP  N+
Sbjct: 896 EELVKIFQELDLQYRLLPLDINVRSLPPVNS 917

BLAST of Cucsa.099120 vs. NCBI nr
Match: gi|449444568|ref|XP_004140046.1| (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis sativus])

HSP 1 Score: 1121.3 bits (2899), Expect = 0.0e+00
Identity = 557/814 (68.43%), Postives = 681/814 (83.66%), Query Frame = 1

Query: 1   MEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRI-VPPLAESPLSQTSKDLKVSFQ 60
           +EDPP+KLIG FL KQK  GE +LDMD+EM EL  ++  +  +AESP+ ++S++LKVSF+
Sbjct: 116 VEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFE 175

Query: 61  HDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQNESLAEAMR 120
             S EIS ND S+RRR+RDS  L EE +G Q PRQ            S  +++  AE +R
Sbjct: 176 SIS-EISEND-SMRRRHRDS-PLDEEHRG-QQPRQCDRRAHG-----SNGEDDGAAEVLR 235

Query: 121 CASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSGQLRSGFLGK-IEDD 180
           C+SN  F  ++SFQRKS+LLRAKTKSRL+DPP   DR SG +PKSGQ+RSG + K ++++
Sbjct: 236 CSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEE 295

Query: 181 DDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWE 240
           DDDPFLE+DLPDE+K+ N   LT+LQW SLI+I AAL+CTL++ Y R K LW+L++WKWE
Sbjct: 296 DDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWE 355

Query: 241 VMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHL 300
           VMI +LICGRLVSGWGIR+ VFFIERNFLLRKRVLYFVYGVRK VQNCLWLGLVLIAW+ 
Sbjct: 356 VMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNF 415

Query: 301 LFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLF 360
           LF+ +V+++  ++ L YV++VLVCLL+STL+WLVKTLMVKVLASSFHVSTYFDRIQ++LF
Sbjct: 416 LFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALF 475

Query: 361 NQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASI-KSGREIG 420
           NQYVIETLSGPPL+EI+KNEEEEER+A+EV KLQNAG  IPPDLKA  F++  K GR IG
Sbjct: 476 NQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIG 535

Query: 421 SGRTHKSFCAKSCKLSRALTKNRND-GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSIST 480
           SG   KS   +S KLSR L+K   D GITIDHLHKLS KNVSAWNMKRL+NIVR+G++ST
Sbjct: 536 SGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLST 595

Query: 481 LDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKT 540
           LDEQI+    +DESTTEIKSE EAK AAKKIF NVA  G KYIYL+DL+RFM +DE  KT
Sbjct: 596 LDEQIKDTAHEDESTTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKT 655

Query: 541 MSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILI 600
           M LFEGA E+++ISKS+LKNWVVNAFRERRALALTLNDTKTAV+KLH MVN++  ++IL+
Sbjct: 656 MGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILV 715

Query: 601 LWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQ 660
           +WL++LGIA+SKF +F++SQ+V+VAF+FGNTCKT+FE+IIFLFVMHPFDVGDRCEIDG+Q
Sbjct: 716 IWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQ 775

Query: 661 MVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKIT 720
           M+VEEMNILTT+FLRYDN K+I PNSVLATK IHNFYRSPDMG+ IEF +HI+TP EKI 
Sbjct: 776 MIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIA 835

Query: 721 AMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLV 780
            M+ RIISYIEG KEHW PAPMIV KD++ LN++++A+WL+HRMNHQD GERW RR++LV
Sbjct: 836 IMRQRIISYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLV 895

Query: 781 EEVVKVCQELDIQYRLLPIDINIRSLPSSNTITI 811
           EE+VK+ QELD+QYRLLP+DIN+RSLP  N+  +
Sbjct: 896 EELVKIFQELDLQYRLLPLDINVRSLPPVNSTNL 920

BLAST of Cucsa.099120 vs. NCBI nr
Match: gi|1009150892|ref|XP_015893266.1| (PREDICTED: mechanosensitive ion channel protein 6-like [Ziziphus jujuba])

HSP 1 Score: 1081.6 bits (2796), Expect = 0.0e+00
Identity = 540/840 (64.29%), Postives = 670/840 (79.76%), Query Frame = 1

Query: 2   EDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRIVPPLAESPLSQTS----KDLKVS 61
           +DPP+KLIG FL KQK  G+ +LDMDLEM+EL+  R +PPLAESP  ++S    K+LKVS
Sbjct: 141 DDPPSKLIGQFLHKQKASGDMSLDMDLEMDELRHERNLPPLAESPAKRSSFGQSKELKVS 200

Query: 62  FQHDSTEISSND------QSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPTISGVQN 121
           FQ  +T  S++D      +S+RRRN+DS +  E+S GG                 SG  N
Sbjct: 201 FQPTTTAASNDDVEIRLSESVRRRNKDSTE--EDSSGG-----------------SGRGN 260

Query: 122 ESLAEAMRCASNLSFHSELSFQRKSNLLRAK-TKSRLIDPPAEPDRLSGLIPKSGQ---- 181
               E +RC SN +F  E+SFQ KS+LLR K TKSRLIDPP EP+  +  +PKSGQ    
Sbjct: 261 GD-DEVVRCTSNAAFEREVSFQSKSSLLRLKKTKSRLIDPPEEPENRASRVPKSGQVMKS 320

Query: 182 --------LRSGFLGK--IEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALI 241
                   LRSG LG   ++DDDDDPF EDDLPD++K+ N SALT+LQW+SLI+I     
Sbjct: 321 GQMLKSGQLRSGMLGNWPLDDDDDDPFWEDDLPDDYKKANLSALTLLQWLSLIVIIGVFA 380

Query: 242 CTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFV 301
           CTLS+P+LR K+ W+L +WKWEV++ +LICGRL SGWG+RI VFFIERNFLLRKRVLYFV
Sbjct: 381 CTLSIPFLRRKNWWKLKLWKWEVLVLVLICGRLFSGWGVRIVVFFIERNFLLRKRVLYFV 440

Query: 302 YGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLM 361
           YG+RK VQNCLWLGLVL+AWH LF+++VE++T +  L YV++VLVCLL+ TL+WLVKTL+
Sbjct: 441 YGLRKAVQNCLWLGLVLLAWHFLFDEKVERETKSDKLKYVTKVLVCLLVGTLVWLVKTLI 500

Query: 362 VKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGI 421
           VKVLASSFHVS YFDRIQESLFNQYVIETLSGPPL+E+++ EEE+ER+ DEV+KLQNAG 
Sbjct: 501 VKVLASSFHVSKYFDRIQESLFNQYVIETLSGPPLIEMKRTEEEDERLVDEVRKLQNAGA 560

Query: 422 IIPPDLKAATFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKN 481
            IPPDLK A F + KSGR IGSG   KS   +S K S+ L+K ++DGITIDHLHKL+ KN
Sbjct: 561 TIPPDLKNAAFPTAKSGRVIGSGGLQKS-PRRSNKFSQPLSKKQDDGITIDHLHKLNPKN 620

Query: 482 VSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGY 541
           VSAWNMKRL+NIVR+GS++TLDEQI+    DDE  T+IKSE EAKAAAKKIFQNVA  G 
Sbjct: 621 VSAWNMKRLMNIVRHGSLTTLDEQIQDSTRDDEKATQIKSEVEAKAAAKKIFQNVARHGS 680

Query: 542 KYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTK 601
           K+IYL DL RF+R+DE LKTMSLFEGA+E+ +ISK++LKNWVVNAFRERRALALTLNDTK
Sbjct: 681 KHIYLRDLTRFLRDDEALKTMSLFEGASESGKISKTSLKNWVVNAFRERRALALTLNDTK 740

Query: 602 TAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAII 661
           TAV+KLHHMV +I   +I ++WL++LGIA++KF +F+SSQ+V+VAFIFGNTCKT+FEAII
Sbjct: 741 TAVNKLHHMVKIIIIFVIGVIWLLILGIATTKFLLFVSSQLVLVAFIFGNTCKTVFEAII 800

Query: 662 FLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSP 721
           FLFV+HPFDVGDRCEIDG+QMVVEEMNILTTVFLRYDN K++ PNSVL+TK I+N+YRSP
Sbjct: 801 FLFVVHPFDVGDRCEIDGVQMVVEEMNILTTVFLRYDNTKIVFPNSVLSTKPINNYYRSP 860

Query: 722 DMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWL 781
           DMG+++EF +H++TPAE+I A+KHRI SYIE  KEHW   PMIV KD++ LN+V+ AVWL
Sbjct: 861 DMGDAVEFCIHVSTPAEEIAAIKHRITSYIENKKEHWCTQPMIVMKDVEELNRVRFAVWL 920

Query: 782 SHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSNTITIEISWNS 817
           +H+MN QD GERW RRS+LVEE++K+ +ELD+QYRL P+DINI S+P   +  +  +W +
Sbjct: 921 NHKMNFQDIGERWMRRSLLVEEMIKIFRELDLQYRLFPLDINICSMPPVTSTRVPTNWTA 959