CSPI07G01240 (gene) Wild cucumber (PI 183967)

NameCSPI07G01240
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionMechanosensitive ion channel family protein
LocationChr7 : 1142322 .. 1146360 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATACGCTCAAGAAATCTTTCAAAGGCAATGTATCTTTCAAGCATACAAGGAAAATTTCTGCTGGAGGAGCCGGCAGCGAAATCAATCACGAAGAGCTCCCCATTCTTCTCAATCACCAAACTACGGATCATAGTCGTTGTCTTAGGGATCGTCATCCGGTGAACGATTCTGATACATCTGACAGAACAGAGGTCATTCTCAAGATTGATGGTGGTGGTTCGTCTTCAGCTGTCTCCAGATCTCTGGACTCTGCTGGCAATAATGGCGGGAATGTTTGGCGGGAATCCAGGTACGATTTCTGGAACAATGATGAAATTGGAATTGGGGAAAGTGCGAGAATGGGTGATAATAGTGGTGTTGATAGAAACGAGGGATTTGAATTTGTACAGACTGGTTATGGAATGGAGGATCCTCCTACGAAGCTGATTGGTGACTTTCTTCGTAAGCAGAAAATTGAGGGAGAAACGACTTTGGATATGGATTTGGAAATGGAGGAATTGAAGCCAAATAGGATTGTACCTCCATTGGCAGAGTCGCCTTTGAGTCAAACTTCTAAGGATCTTAAAGTTTCGTTTCAGCACGACTCGACAGAAATTTCGAGCAATGATCAGTCAATAAGAAGGCGGAATAGAGATTCAAATGATTTGAAAGAAGAGTCTAAAGGAGGACAATCGCCACGGCAACAACCGCACCATGAACGTCTTGGATCTCCAACTATCTCTGGGGTTCAGAACGAGTCTCTTGCTGAGGCTATGAGATGCGCATCTAACTTGTCCTTTCATAGTGAGCTTTCATTTCAAAGGAAATCTAATTTACTGAGGGCGAAAACCAAGTCAAGATTGATAGACCCACCTGCCGAGCCTGACCGGCTCTCTGGCCTCATTCCTAAGTCAGGTCAACTACGATCTGGGTTTCTAGGGAAGATTGAAGACGACGACGATGACCCCTTTCTAGAGGATGACCTTCCAGACGAATTTAAAAGGGGAAATTTCAGTGCTCTAACTGTTCTACAATGGGTTAGTTTGATTATAATCACTGCAGCTTTAATCTGCACTCTTTCTGTTCCTTATTTGAGGGAAAAGAGTCTATGGGAGTTGGATATATGGAAATGGGAGGTGATGATTTTTATACTGATTTGTGGACGATTGGTATCTGGTTGGGGGATTAGGATTGGTGTATTTTTCATTGAGAGGAATTTTCTTTTGCGTAAAAGGGTTTTGTATTTTGTATATGGGGTTAGAAAACCAGTGCAGAATTGTTTATGGTTAGGCCTTGTTCTCATCGCTTGGCATTTGTTGTTCAATAAACGGGTTGAGAAACAAACCAACACCAGTATACTCAATTATGTGAGCAGAGTTTTAGTTTGTCTCTTGATAAGCACACTGATATGGCTTGTGAAAACCTTGATGGTGAAGGTGCTTGCCTCTTCTTTCCATGTAAGCACATACTTCGATCGAATTCAGGAATCGTTGTTTAATCAATATGTCATTGAGACGCTCTCGGGTCCACCTCTGGTTGAAATACGGAAGAATGAGGAAGAAGAGGAGAGGATTGCAGATGAAGTCCAAAAATTACAGAATGCAGGAATTATCATACCCCCTGATCTCAAGGCTGCAACCTTTGCTTCTATAATGAGTGGAAGGGAAATAGGTAGTGGACGGACCCACAAAAGTTTTTGTGCAAAAAGTTGCAAACTCTCTCGAGCACTGACCAAAAATAGGAACGATGGAATAACGATTGACCATTTGCACAAACTAAGTACGAAGAACGTCTCTGCCTGGAATATGAAGAGGTTGTTGAACATAGTTCGATATGGTAGTATTTCCACACTGGATGAGCAAATACGGGGGCCGTGTCTTGATGATGAATCTACTACAGAGATCAAAAGTGAACGCGAGGCAAAGGCTGCAGCAAAAAAGATTTTTCAGAATGTGGCTCATCGTGGATATAAGTATGTTGTTATATCTATTATATCGACACTCATGTGGGCTTGAAAATTTGTTGAAGCCCTAACATATGGAATTTAATATCAATCGGCAAGAAAGTAATTTTATGGGGCTCAAACTCATGACCTTCACCATGTTAAATCAACAACAATTCAAAAGTTTAAGTTGACGAGTTGGGATAAATCTAATATCAATACTCTTACATATGCCATTTCTGCTCATATTAAGCTTTCTTATGGCATATGACTCATATCCTATGAAACTAACCCTCCTAATCACGTCTCCATGTATGTTCCTTGTTGCTTGACAAAGTGAAATTCTTTTGAGAGTCTAATTCAGGTTAACCAGCTACTTAGGTTTAATTATGTTGAGGGATTTCTTGATACTCAAATATTATAGAGTCGGATAGATTGCTTCACGAGATTAGCCAAGATACTTATAGATAGGTTTGCATACTTACATATATTAAAAAAAAAGACATGCATTGTTATGTTTACGCTAACCTCATTACACAATTTCTTTTGTTCTTTGCAGGTACATATACCTGGATGACTTAGTGCGGTTCATGAGAGAAGACGAGGTTCTTAAAACGATGAGTCTCTTTGAAGGAGCCGCTGAAAACCAGAGGATAAGCAAATCTGCCTTGAAAAATTGGGTGGTAAGAATGCAAAAATGATTTCTGAAAACGTTCCTTATCAGTTATCTCTTTTGAGTTAGTGCCATTGCTCGTGTTACAAGTAAATGAAGTTACTGAGATGAAATAGTATGACTAGCACTGTGCTGGGAAATTTGTTTAGAAATTCTGTTTACATCTAGATAATTAAAACCACTGCAGCGAGAAAGTTTAAGAATCGTTGTCTGAAATTATGGAGATAAATTAATCTTAATGAAGCTTTGTCCGCAATTATGCTCCAAAATACTCCAAATGTTATCCATGATCGTTCCAACTTCCAATTTCTGATCAAATTTCTTCCAATTCTAGTGCTTTAGCATTTTCACCGCCTATTCTTGACATTATGGTACATCCTTCTGTCCAGGTCAATGCGTTCAGGGAACGAAGAGCCCTTGCTTTAACACTGAATGATACCAAAACAGCTGTGGATAAACTACATCACATGGTGAATGTCATATTCGGCATCCTTATATTAATTTTATGGCTTATAGTATTAGGAATTGCCTCCAGCAAATTTTTCATCTTCCTTAGTTCTCAAATAGTGGTTGTGGCATTTATTTTTGGAAACACTTGCAAAACCATATTTGAAGCAATCATCTTTTTGTTTGTCATGCATCCGTTTGACGTTGGAGATCGCTGCGAAATCGATGGAATGCAGGTAGCTCTCATTTGTTTTGATTTTTATGTAACTCTAATTTCTTCTGAACCTTGGACCTGATTATCATCCCACTGAAGTGTAGATGGTTGTAGAGGAAATGAACATTTTGACTACTGTATTCTTGAGATACGACAACTTGAAGGTTATAATCCCAAATAGTGTTCTTGCAACCAAATTAATCCACAACTTCTACCGTAGTCCTGACATGGGCGAATCAATTGAATTCCTTGTCCATATCGCTACACCAGCTGAGAAAATTACGGCCATGAAACACAGAATTATAAGGTAATTGTTAGCTGTAAAAGAACAATGTTGTTACCTGACATACGTAATAGCTGACTATTTTTCTTCTGCAGTTACATTGAAGGCAACAAAGAACATTGGTATCCTGCTCCTATGATTGTGTTCAAAGATATAGATGGTTTAAACAAGGTCAAATTGGCAGTTTGGTTGTCACACAGAATGAACCATCAAGACTCAGGGGAAAGATGGGCTCGGAGGTCCGTCTTGGTCGAAGAAGTTGTCAAAGTCTGTCAAGAGCTCGACATTCAATATCGTCTATTGCCAATTGATATCAATATCCGTTCATTGCCTTCTTCTGCACCCTCCATTGGCTTTCCTTCAAATTGGACTTCCCCTGCAAGTTGAACAGCCAGCAACACAGAAGGCTTAACTTTCATCCTCTCTCAGATAAGCTTTCTCATGTCCTATCATCTTCATTGTAGAGATTCACCAATCATCATTTCGACGAGAGGACA

mRNA sequence

ATGGATACGCTCAAGAAATCTTTCAAAGGCAATGTATCTTTCAAGCATACAAGGAAAATTTCTGCTGGAGGAGCCGGCAGCGAAATCAATCACGAAGAGCTCCCCATTCTTCTCAATCACCAAACTACGGATCATAGTCGTTGTCTTAGGGATCGTCATCCGGTGAACGATTCTGATACATCTGACAGAACAGAGGTCATTCTCAAGATTGATGGTGGTGGTTCGTCTTCAGCTGTCTCCAGATCTCTGGACTCTGCTGGCAATAATGGCGGGAATGTTTGGCGGGAATCCAGGTACGATTTCTGGAACAATGATGAAATTGGAATTGGGGAAAGTGCGAGAATGGGTGATAATAGTGGTGTTGATAGAAACGAGGGATTTGAATTTGTACAGACTGGTTATGGAATGGAGGATCCTCCTACGAAGCTGATTGGTGACTTTCTTCGTAAGCAGAAAATTGAGGGAGAAACGACTTTGGATATGGATTTGGAAATGGAGGAATTGAAGCCAAATAGGATTGTACCTCCATTGGCAGAGTCGCCTTTGAGTCAAACTTCTAAGGATCTTAAAGTTTCGTTTCAGCACGACTCGACAGAAATTTCGAGCAATGATCAGTCAATAAGAAGGCGGAATAGAGATTCAAATGATTTGAAAGAAGAGTCTAAAGGAGGACAATCGCCACGGCAACAACCGCACCATGAACGTCTTGGATCTCCAACTATCTCTGGGGTTCAGAACGAGTCTCTTGCTGAGGCTATGAGATGCGCATCTAACTTGTCCTTTCATAGTGAGCTTTCATTTCAAAGGAAATCTAATTTACTGAGGGCGAAAACCAAGTCAAGATTGATAGACCCACCTGCCGAGCCTGACCGGCTCTCTGGCCTCATTCCTAAGTCAGGTCAACTACGATCTGGGTTTCTAGGGAAGATTGAAGACGACGACGATGACCCCTTTCTAGAGGATGACCTTCCAGACGAATTTAAAAGGGGAAATTTCAGTGCTCTAACTGTTCTACAATGGGTTAGTTTGATTATAATCACTGCAGCTTTAATCTGCACTCTTTCTGTTCCTTATTTGAGGGAAAAGAGTCTATGGGAGTTGGATATATGGAAATGGGAGGTGATGATTTTTATACTGATTTGTGGACGATTGGTATCTGGTTGGGGGATTAGGATTGGTGTATTTTTCATTGAGAGGAATTTTCTTTTGCGTAAAAGGGTTTTGTATTTTGTATATGGGGTTAGAAAACCAGTGCAGAATTGTTTATGGTTAGGCCTTGTTCTCATCGCTTGGCATTTGTTGTTCAATAAACGGGTTGAGAAACAAACCAACACCAGTATACTCAATTATGTGAGCAGAGTTTTAGTTTGTCTCTTGATAAGCACACTGATATGGCTTGTGAAAACCTTGATGGTGAAGGTGCTTGCCTCTTCTTTCCATGTAAGCACATACTTCGATCGAATTCAGGAATCGTTGTTTAATCAATATGTCATTGAGACGCTCTCGGGTCCACCTCTGGTTGAAATACGGAAGAATGAGGAAGAAGAGGAGAGGATTGCAGATGAAGTCCAAAAATTACAGAATGCAGGAATTATCATACCCCCTGATCTCAAGGCTGCAACCTTTGCTTCTATAATGAGTGGAAGGGAAATAGGTAGTGGACGGACCCACAAAAGTTTTTGTGCAAAAAGTTGCAAACTCTCTCGAGCACTGACCAAAAATAGGAACGATGGAATAACGATTGACCATTTGCACAAACTAAGTACGAAGAACGTCTCTGCCTGGAATATGAAGAGGTTGTTGAACATAGTTCGATATGGTAGTATTTCCACACTGGATGAGCAAATACGGGGGCCGTGTCTTGATGATGAATCTACTACAGAGATCAAAAGTGAACGCGAGGCAAAGGCTGCAGCAAAAAAGATTTTTCAGAATGTGGCTCATCGTGGATATAAGTACATATACCTGGATGACTTAGTGCGGTTCATGAGAGAAGACGAGGTTCTTAAAACGATGAGTCTCTTTGAAGGAGCCGCTGAAAACCAGAGGATAAGCAAATCTGCCTTGAAAAATTGGGTGGTCAATGCGTTCAGGGAACGAAGAGCCCTTGCTTTAACACTGAATGATACCAAAACAGCTGTGGATAAACTACATCACATGGTGAATGTCATATTCGGCATCCTTATATTAATTTTATGGCTTATAGTATTAGGAATTGCCTCCAGCAAATTTTTCATCTTCCTTAGTTCTCAAATAGTGGTTGTGGCATTTATTTTTGGAAACACTTGCAAAACCATATTTGAAGCAATCATCTTTTTGTTTGTCATGCATCCGTTTGACGTTGGAGATCGCTGCGAAATCGATGGAATGCAGATGGTTGTAGAGGAAATGAACATTTTGACTACTGTATTCTTGAGATACGACAACTTGAAGGTTATAATCCCAAATAGTGTTCTTGCAACCAAATTAATCCACAACTTCTACCGTAGTCCTGACATGGGCGAATCAATTGAATTCCTTGTCCATATCGCTACACCAGCTGAGAAAATTACGGCCATGAAACACAGAATTATAAGTTACATTGAAGGCAACAAAGAACATTGGTATCCTGCTCCTATGATTGTGTTCAAAGATATAGATGGTTTAAACAAGGTCAAATTGGCAGTTTGGTTGTCACACAGAATGAACCATCAAGACTCAGGGGAAAGATGGGCTCGGAGGTCCGTCTTGGTCGAAGAAGTTGTCAAAGTCTGTCAAGAGCTCGACATTCAATATCGTCTATTGCCAATTGATATCAATATCCGTTCATTGCCTTCTTCTGCACCCTCCATTGGCTTTCCTTCAAATTGGACTTCCCCTGCAAGTTGA

Coding sequence (CDS)

ATGGATACGCTCAAGAAATCTTTCAAAGGCAATGTATCTTTCAAGCATACAAGGAAAATTTCTGCTGGAGGAGCCGGCAGCGAAATCAATCACGAAGAGCTCCCCATTCTTCTCAATCACCAAACTACGGATCATAGTCGTTGTCTTAGGGATCGTCATCCGGTGAACGATTCTGATACATCTGACAGAACAGAGGTCATTCTCAAGATTGATGGTGGTGGTTCGTCTTCAGCTGTCTCCAGATCTCTGGACTCTGCTGGCAATAATGGCGGGAATGTTTGGCGGGAATCCAGGTACGATTTCTGGAACAATGATGAAATTGGAATTGGGGAAAGTGCGAGAATGGGTGATAATAGTGGTGTTGATAGAAACGAGGGATTTGAATTTGTACAGACTGGTTATGGAATGGAGGATCCTCCTACGAAGCTGATTGGTGACTTTCTTCGTAAGCAGAAAATTGAGGGAGAAACGACTTTGGATATGGATTTGGAAATGGAGGAATTGAAGCCAAATAGGATTGTACCTCCATTGGCAGAGTCGCCTTTGAGTCAAACTTCTAAGGATCTTAAAGTTTCGTTTCAGCACGACTCGACAGAAATTTCGAGCAATGATCAGTCAATAAGAAGGCGGAATAGAGATTCAAATGATTTGAAAGAAGAGTCTAAAGGAGGACAATCGCCACGGCAACAACCGCACCATGAACGTCTTGGATCTCCAACTATCTCTGGGGTTCAGAACGAGTCTCTTGCTGAGGCTATGAGATGCGCATCTAACTTGTCCTTTCATAGTGAGCTTTCATTTCAAAGGAAATCTAATTTACTGAGGGCGAAAACCAAGTCAAGATTGATAGACCCACCTGCCGAGCCTGACCGGCTCTCTGGCCTCATTCCTAAGTCAGGTCAACTACGATCTGGGTTTCTAGGGAAGATTGAAGACGACGACGATGACCCCTTTCTAGAGGATGACCTTCCAGACGAATTTAAAAGGGGAAATTTCAGTGCTCTAACTGTTCTACAATGGGTTAGTTTGATTATAATCACTGCAGCTTTAATCTGCACTCTTTCTGTTCCTTATTTGAGGGAAAAGAGTCTATGGGAGTTGGATATATGGAAATGGGAGGTGATGATTTTTATACTGATTTGTGGACGATTGGTATCTGGTTGGGGGATTAGGATTGGTGTATTTTTCATTGAGAGGAATTTTCTTTTGCGTAAAAGGGTTTTGTATTTTGTATATGGGGTTAGAAAACCAGTGCAGAATTGTTTATGGTTAGGCCTTGTTCTCATCGCTTGGCATTTGTTGTTCAATAAACGGGTTGAGAAACAAACCAACACCAGTATACTCAATTATGTGAGCAGAGTTTTAGTTTGTCTCTTGATAAGCACACTGATATGGCTTGTGAAAACCTTGATGGTGAAGGTGCTTGCCTCTTCTTTCCATGTAAGCACATACTTCGATCGAATTCAGGAATCGTTGTTTAATCAATATGTCATTGAGACGCTCTCGGGTCCACCTCTGGTTGAAATACGGAAGAATGAGGAAGAAGAGGAGAGGATTGCAGATGAAGTCCAAAAATTACAGAATGCAGGAATTATCATACCCCCTGATCTCAAGGCTGCAACCTTTGCTTCTATAATGAGTGGAAGGGAAATAGGTAGTGGACGGACCCACAAAAGTTTTTGTGCAAAAAGTTGCAAACTCTCTCGAGCACTGACCAAAAATAGGAACGATGGAATAACGATTGACCATTTGCACAAACTAAGTACGAAGAACGTCTCTGCCTGGAATATGAAGAGGTTGTTGAACATAGTTCGATATGGTAGTATTTCCACACTGGATGAGCAAATACGGGGGCCGTGTCTTGATGATGAATCTACTACAGAGATCAAAAGTGAACGCGAGGCAAAGGCTGCAGCAAAAAAGATTTTTCAGAATGTGGCTCATCGTGGATATAAGTACATATACCTGGATGACTTAGTGCGGTTCATGAGAGAAGACGAGGTTCTTAAAACGATGAGTCTCTTTGAAGGAGCCGCTGAAAACCAGAGGATAAGCAAATCTGCCTTGAAAAATTGGGTGGTCAATGCGTTCAGGGAACGAAGAGCCCTTGCTTTAACACTGAATGATACCAAAACAGCTGTGGATAAACTACATCACATGGTGAATGTCATATTCGGCATCCTTATATTAATTTTATGGCTTATAGTATTAGGAATTGCCTCCAGCAAATTTTTCATCTTCCTTAGTTCTCAAATAGTGGTTGTGGCATTTATTTTTGGAAACACTTGCAAAACCATATTTGAAGCAATCATCTTTTTGTTTGTCATGCATCCGTTTGACGTTGGAGATCGCTGCGAAATCGATGGAATGCAGATGGTTGTAGAGGAAATGAACATTTTGACTACTGTATTCTTGAGATACGACAACTTGAAGGTTATAATCCCAAATAGTGTTCTTGCAACCAAATTAATCCACAACTTCTACCGTAGTCCTGACATGGGCGAATCAATTGAATTCCTTGTCCATATCGCTACACCAGCTGAGAAAATTACGGCCATGAAACACAGAATTATAAGTTACATTGAAGGCAACAAAGAACATTGGTATCCTGCTCCTATGATTGTGTTCAAAGATATAGATGGTTTAAACAAGGTCAAATTGGCAGTTTGGTTGTCACACAGAATGAACCATCAAGACTCAGGGGAAAGATGGGCTCGGAGGTCCGTCTTGGTCGAAGAAGTTGTCAAAGTCTGTCAAGAGCTCGACATTCAATATCGTCTATTGCCAATTGATATCAATATCCGTTCATTGCCTTCTTCTGCACCCTCCATTGGCTTTCCTTCAAATTGGACTTCCCCTGCAAGTTGA
BLAST of CSPI07G01240 vs. Swiss-Prot
Match: MSL6_ARATH (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana GN=MSL6 PE=1 SV=1)

HSP 1 Score: 911.0 bits (2353), Expect = 1.1e-263
Identity = 503/910 (55.27%), Postives = 636/910 (69.89%), Query Frame = 1

Query: 59  DTSDRTEVILKIDG-GGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIG------- 118
           D +DR EVI+KIDG  G+++ VS      G   G +WR+  YDFW + E  +        
Sbjct: 4   DAADRREVIVKIDGENGNNNGVS------GETVGKIWRDGSYDFWTDGEGNLNKGHNAAA 63

Query: 119 -ESARMGDNSGVD-RNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEEL 178
            +S R    +G   ++EGFEF +     EDPPTKLIG FL KQ+  GE  LDMDL M+EL
Sbjct: 64  VDSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLGMDEL 123

Query: 179 KPNRIVPPLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPR 238
           + +R + P++ESP   T +D           +   D      N D  ++ + S G  +P 
Sbjct: 124 Q-SRGLTPVSESPRVSTKRD----------PVGRRDSRSNTNNNDDGEVVKCS-GNNAPI 183

Query: 239 QQPHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAE 298
           Q               ++ S    MR  S LS         ++    A  KS  I  P  
Sbjct: 184 Q---------------RSSSTLLKMRTRSRLSDPPTPQLPPQT----ADMKSGRI--PKS 243

Query: 299 PDRLSGLIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITA 358
               SG   KS + +       E+++DDPF  +DLP+E+++   S   VL+W+SLI+I A
Sbjct: 244 GQMKSGFFGKSPKTQG------EEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSLILIIA 303

Query: 359 ALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVL 418
             +CTL++P LR+K LWEL +WKWE M+ +LICGRLVS W ++I VFFIERNFLLRKRVL
Sbjct: 304 GFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVL 363

Query: 419 YFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVK 478
           YFVYGVRK VQNCLWLGLVL+AWH LF+++V K  NT  L  V+++ VCLL+  L+WLVK
Sbjct: 364 YFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCLLVGFLLWLVK 423

Query: 479 TLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQN 538
           TL+VKVLASSFH+STYFDRIQESLF QYVIETLSGPPL+EI+KNEEEEERI+ EV+K QN
Sbjct: 424 TLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEVKKFQN 483

Query: 539 AGIIIPPDLKAATFASIMSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLS 598
            G +            I SG +    +T KS        +       N GITID LHKL+
Sbjct: 484 PGGV-----------EIQSGAQKSPMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLN 543

Query: 599 TKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAH 658
            KNVSAW MKRL+NI+R GS++TLDEQ++ P LDD+   +I+SE EAK AA+KIF NVA 
Sbjct: 544 PKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFEAKLAARKIFHNVAK 603

Query: 659 RGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLN 718
            G K+IY +D++RF+ +DE LKT+SLFEGA+E  RISKS+LKNWVVNAFRERRALALTLN
Sbjct: 604 PGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLN 663

Query: 719 DTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFE 778
           DTKTAV++LH MVN++ GI+IL++WLI+LGI S+KF + +SSQ+VVVAFIFGN CK +FE
Sbjct: 664 DTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFE 723

Query: 779 AIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFY 838
           +II+LFV+HPFDVGDRCEIDG+QMVVEEMNILTTVFLR+DN KV+ PNS+L TK I N+Y
Sbjct: 724 SIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYY 783

Query: 839 RSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLA 898
           RSPDMG+ IEF +HI TPAEKI  +K RI SYIEG K+HWYPAPMIVFKD++ LN V++A
Sbjct: 784 RSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIA 843

Query: 899 VWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAP---SIG 956
           VW +HRMNHQD GE+WARRS LVEE+ K+C+ELDI+YRL P+DIN+R+LP+S     S  
Sbjct: 844 VWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDINVRNLPTSTALPVSDR 853

BLAST of CSPI07G01240 vs. Swiss-Prot
Match: MSL8_ARATH (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana GN=MSL8 PE=2 SV=2)

HSP 1 Score: 890.2 bits (2299), Expect = 2.0e-257
Identity = 500/968 (51.65%), Postives = 665/968 (68.70%), Query Frame = 1

Query: 5   KKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDTSDRT 64
           + SFK + S+K  R   + G  SE + E LPIL +H        + D+ P  DS  S   
Sbjct: 4   RNSFKSHSSYKQIR---SPGDQSEPSPEHLPILHDHHPDHSGMVVDDQKP--DSTRS--- 63

Query: 65  EVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGE---SARMGDNSGV 124
                            SLD   N    V R++ Y FW ++  G      + R  D   +
Sbjct: 64  -----------------SLDDGRN--APVERDASYKFWQDNTTGTSTDHTAVRTSDKDPI 123

Query: 125 ------DRNEG-FEFVQTGYGMEDPPTKLI-GDFLRKQ---KIEGETTLDMDLEMEELKP 184
                 DR  G F+FV     +++ PTK++ G+ + +Q   +   E TLD+D E +++  
Sbjct: 124 AISRKGDRLSGSFDFVHGKLPVDESPTKMVAGEPVNRQWRGRNNEEITLDVDQENDDVSH 183

Query: 185 NRIVPPLAESPLS-QTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQ 244
             +  P + +  S   S++++VSF            ++RR              G  P  
Sbjct: 184 QTMPTPTSTARTSFDASREMRVSF------------NVRRAGG-------AFVAGSVPSS 243

Query: 245 QPHHERLGSPTISGVQNESLA---EAMRCASNLSFHSELSFQRKSNLL-RAKTKSRLIDP 304
             H     S T+   Q++      E +RC SN+SF      QRKS L+ R KT+SRL DP
Sbjct: 244 SSHSSSSSSATMRTNQDQPQLQEEEVVRCTSNMSF------QRKSELISRVKTRSRLQDP 303

Query: 305 PAEPDR-LSGLIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLI 364
           P E +   SG   +SGQL+SG L  I D++DDP  E+D+PDE+KRG   A+T+LQW+SL+
Sbjct: 304 PREEETPYSGW--RSGQLKSGLLADI-DEEDDPLAEEDVPDEYKRGKLDAITLLQWLSLV 363

Query: 365 IITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLR 424
            I AAL C+LS+   ++  +W L +WKWEV + +LICGRLVSGWGIRI VFFIERNFLLR
Sbjct: 364 AIIAALACSLSIQSWKKVRVWNLHLWKWEVFLLVLICGRLVSGWGIRIVVFFIERNFLLR 423

Query: 425 KRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLI 484
           KRVLYFVYGVR+ VQNCLWLGLVL+AWH LF+K+V+++T +  L YV+++LVC L+ST++
Sbjct: 424 KRVLYFVYGVRRAVQNCLWLGLVLLAWHFLFDKKVQRETRSRFLPYVTKILVCFLLSTIL 483

Query: 485 WLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQ 544
           WL+KTL+VKVLASSFHVSTYFDRIQE+LFNQYVIETLSGPP++E+ + EEEEER  DE+ 
Sbjct: 484 WLIKTLVVKVLASSFHVSTYFDRIQEALFNQYVIETLSGPPMIEMSRIEEEEERAQDEIF 543

Query: 545 KLQNAGIIIPPDLKAATFASIMSGREIGSGRTHKSFCAKSCKLSRALTKNRND-GITIDH 604
           K+QNAG  +PPDL AA F    SGR +              KLS  + K+  D GI+++H
Sbjct: 544 KMQNAGANLPPDLCAAAFPPGKSGRVMNP------------KLSPIIPKSTTDNGISMEH 603

Query: 605 LHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIF 664
           LH+++ KN+SAWNMKRL+ IVR  S++TLDEQ+     +DEST +I+SE+EAKAAA+KIF
Sbjct: 604 LHRMNHKNISAWNMKRLMKIVRNVSLTTLDEQMLESTYEDESTRQIRSEKEAKAAARKIF 663

Query: 665 QNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRAL 724
           +NV  RG KYIYL+DL+RF+REDE +KTM LFEGA EN+RISKSALKNW+VNAFRERRAL
Sbjct: 664 KNVEQRGAKYIYLEDLMRFLREDEAMKTMGLFEGAPENKRISKSALKNWLVNAFRERRAL 723

Query: 725 ALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTC 784
           ALTLNDTKTAV+KLHHM+N++  I+I+++WL++L IASSK  +F+SSQ+V++AFIFGNT 
Sbjct: 724 ALTLNDTKTAVNKLHHMINIVTAIVIVVIWLVLLEIASSKVLLFVSSQVVLLAFIFGNTV 783

Query: 785 KTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKL 844
           KT+FE+IIFLF++HP+DVGDRCEID +Q+VVEEMNILTTVFLRYDNLK++ PNS+L  K 
Sbjct: 784 KTVFESIIFLFIVHPYDVGDRCEIDSVQLVVEEMNILTTVFLRYDNLKIMYPNSLLWQKS 843

Query: 845 IHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLN 904
           I+N+YRSPDMG++IEF VHI TP EKI+ +K RI +YI+   E+WYP   I+ KD++ L+
Sbjct: 844 INNYYRSPDMGDAIEFCVHITTPLEKISVIKQRISNYIDNKPEYWYPQAKIIVKDLEDLH 903

Query: 905 KVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPS 952
            V+LA+W  HR+NHQD  ERW RR+VLVEEV+K+  ELDIQ+R  P+DIN+R++P+   S
Sbjct: 904 IVRLAIWPCHRINHQDMAERWTRRAVLVEEVIKILLELDIQHRFYPLDINVRTMPTVVSS 903

BLAST of CSPI07G01240 vs. Swiss-Prot
Match: MSL5_ARATH (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana GN=MSL5 PE=2 SV=1)

HSP 1 Score: 889.8 bits (2298), Expect = 2.6e-257
Identity = 491/932 (52.68%), Postives = 652/932 (69.96%), Query Frame = 1

Query: 59  DTSDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDN 118
           D++DR + I+ I+G   S AV  +  S+   GGN+W+ES YDFW+ ++   G++ + GD+
Sbjct: 5   DSTDRRDFIVNINGQ-ESGAVGATGSSSNAEGGNIWKESSYDFWDGEK---GKNDKKGDD 64

Query: 119 SGVDRNEGFEFVQTGYG------MEDPPTKLIGDFLRKQKIEG-ETTLDMDLEMEELKPN 178
              D    F F Q G        + DPP+KLIG FL KQ+  G E +LD++L M EL+ N
Sbjct: 65  EDEDGGS-FHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSN 124

Query: 179 RIVPPLAES--------PLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKG 238
               P   S         +S++S  +K   + D+          RR+NR S        G
Sbjct: 125 TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVR--------RRQNRTS-------LG 184

Query: 239 GQSPRQQPHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLI 298
           G S  +               +N   AE ++C S           +K  L R KTKSRL 
Sbjct: 185 GSSDEEG--------------RNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQ 244

Query: 299 DPPA-------EPDRLSGLIPKSGQLRSGFLGKI------------EDDDDDPFLEDDLP 358
           DPP        + +  SG   +SG  +SGFLGK             E++++DPFL++DLP
Sbjct: 245 DPPTPTHPAIDKTEMKSGR--RSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLP 304

Query: 359 DEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGRL 418
           +EFKR   S    L+W+SL++I  +L+C+L++  L+ K+ W+LD+WKWEV + +LICGRL
Sbjct: 305 EEFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRL 364

Query: 419 VSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTN 478
           VS W +RI VF +E+NF  RKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+K+VE++T 
Sbjct: 365 VSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETR 424

Query: 479 TSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGP 538
           ++ L YV+RVLVCLL++ +IWLVKT++VKVLASSFH+STYFDRIQESLF QYVIETLSGP
Sbjct: 425 STALRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGP 484

Query: 539 PLVEIRKNEEEEERIADEVQKLQN-AGIIIPPDLKAATFASIMSGREIGSGRTHKSFCAK 598
           PL+EI++ EEEE+++A++V+ L+  AG  +PP LKA    ++ S  ++G          K
Sbjct: 485 PLMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKA----TVKSFMKVG----------K 544

Query: 599 SCKLSRALTKNRNDG--ITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCL 658
           S  L+R  +K   DG  I ID L +++TKNVSAWNMKRL+NI+  G+ISTLD+ ++    
Sbjct: 545 SPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQ 604

Query: 659 DDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAEN 718
           +DE  T I+SE EAK AA+KIF NV   G +YIYL+D +RF+ E+E  + M+LFEGA+E+
Sbjct: 605 EDEDATHIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASES 664

Query: 719 QRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIAS 778
            +ISKS LKNWVV AFRERRALALTLNDTKTAVD+LH ++NV+ GI+I+I+WL++LGIA+
Sbjct: 665 DKISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIAT 724

Query: 779 SKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILT 838
           ++F + LSSQ+++VAF+FGN+CKTIFEAIIFLFVMHPFDVGDRCEIDG+Q+VVEEMNILT
Sbjct: 725 TRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILT 784

Query: 839 TVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYI 898
           TVFLRYDN K+I PNSVL TK I N+YRSPDMG+++EF VHIATP EKITA+K RI+SY+
Sbjct: 785 TVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYV 844

Query: 899 EGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQEL 951
           +  K++WYPAPMIVF  +D LN VK+AVWL+HRMNHQD GER+ RR +L+EEV K C+EL
Sbjct: 845 DNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCREL 875

BLAST of CSPI07G01240 vs. Swiss-Prot
Match: MSL4_ARATH (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana GN=MSL4 PE=3 SV=1)

HSP 1 Score: 849.0 bits (2192), Expect = 5.1e-245
Identity = 462/923 (50.05%), Postives = 640/923 (69.34%), Query Frame = 1

Query: 60  TSDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNS 119
           T  R + +++IDG  +  +               WRES  +FW+ND+          D S
Sbjct: 6   TDQRRDFVVRIDGEDNGDSEK------------FWRESSINFWHNDKSSKPPGGEEDDGS 65

Query: 120 GVDRNEGFEFVQTGYGME---DPPTKLIGDFLRKQKIEG-ETTLDMDLEMEELKPNRIVP 179
                  F+F++         DPP+KLI  FL KQK  G E +LDM+  M EL+ N  VP
Sbjct: 66  -------FDFMRRSSEKSEEPDPPSKLINQFLNKQKASGDEISLDMEANMPELQKNT-VP 125

Query: 180 PLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHER 239
           PL+ + +S ++  +       S    + D   RR+NR +  L    K G S   + +   
Sbjct: 126 PLSSTAVSGSASPVTAPVTA-SYRNGTGDAIRRRQNRVT--LSPSVKDGDSSEDEENR-- 185

Query: 240 LGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAE--PDRLS 299
                + G      +E ++C SN S        R   L++ KT+SRL+DPP    PD +S
Sbjct: 186 -----VDG------SEVVKCTSNRST------MRTKTLMKMKTRSRLMDPPTPTYPDMVS 245

Query: 300 GLIPKSGQLRSGFLG-------------KIEDDDDDPFLEDDLPDEFKRGNFSALTVLQW 359
           G  P+SG L  GF G             K  ++++DPF E+DLP+  ++       +++W
Sbjct: 246 GRTPRSGNLNPGFSGRNTKPGTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEW 305

Query: 360 VSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERN 419
           + LI+I A+LIC+L +PYLR K+LW+L +WKWEVM+ +LICGRLVS W +++ V+F+E N
Sbjct: 306 IFLILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESN 365

Query: 420 FLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLI 479
           FL RK+VLYFVYG+RKPVQNCLWLGLVLIAWH LF+K+VE++  +++L YV++VL+CLL+
Sbjct: 366 FLWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLV 425

Query: 480 STLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIA 539
           + +IWL+KTL+VKVLASSFH+STYFDRIQESLF QYVIETLSGPP +EI     EEE++A
Sbjct: 426 AVIIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEIHI---EEEKVA 485

Query: 540 DEVQKLQNAGIIIPP-DLKAATFASIMSGREIGSGRTHKS--FCAKSCKLSRALTKNRN- 599
           ++V+  +  G  + P   KA +    ++   +GSGR  KS     KS  LSR+ +K    
Sbjct: 486 NDVKTFEIVGRKLSPLGPKAVSSPPQVT---VGSGRLQKSPSRVGKSPVLSRSGSKKEGG 545

Query: 600 -DGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCL-DDESTTEIKSERE 659
            +GI IDHL +++TKNVSAW MK+L+N+++ G++STLDEQI+     +D+  T+I+SE E
Sbjct: 546 EEGIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFE 605

Query: 660 AKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVV 719
           AK AA+KIFQNVA  G +YIY++D +RF+ EDE  + M LFEGA+E  +ISKS LKNWVV
Sbjct: 606 AKLAARKIFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVV 665

Query: 720 NAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVV 779
           NAFRERRALALTLNDTKTAV++LH +V+V+  I+ILI+WL++LGIA++KF + +SSQ+++
Sbjct: 666 NAFRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLL 725

Query: 780 VAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVII 839
           V F+FGN+CKTIFEA+IF+FVMHPFDVGDRCEIDG+QM+VEEMNILTTVFLR+DN K++ 
Sbjct: 726 VVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVY 785

Query: 840 PNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMI 899
           PNS+L TK I N+YRSPDM ++IEF VHIATP EK TA++ RI+SY++  K+HW+P+PMI
Sbjct: 786 PNSLLGTKPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMI 845

Query: 900 VFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINI 956
           VF+D+ GLN VK+A+W +H+MNHQ+ GER+ RR  L+EE+ ++C+ELDI+YRL P++IN+
Sbjct: 846 VFRDMCGLNSVKIAMWPTHKMNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINV 880

BLAST of CSPI07G01240 vs. Swiss-Prot
Match: MSL7_ARATH (Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana GN=MSL7 PE=3 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 2.9e-224
Identity = 398/689 (57.76%), Postives = 531/689 (77.07%), Query Frame = 1

Query: 269 RKSNLL-RAKTKSRLIDPPAEPDR-LSGLIPKSGQLRSGFLGKIEDD---DDDPFLEDDL 328
           RK+ L+ RAK +SRLIDPP E ++  S  I  S QLRSG LG+  DD   +DD   E+D+
Sbjct: 161 RKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSAEEDV 220

Query: 329 PDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGR 388
           P E+++    A+T+LQW+SLI +  AL+ +L +   R  +LW L +WKWEV++ +LICGR
Sbjct: 221 PVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVLICGR 280

Query: 389 LVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQT 448
           LVSG GIRI VFFIERNFLLRKRVLYFVYGV+  VQNCLWLGLVL+AWH LF+K+VEK+T
Sbjct: 281 LVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKVEKET 340

Query: 449 NTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSG 508
            + +L  +S++LVC L+ST++WL+KTL+VKVLASSFHVSTYFDRIQE+LF+ Y+IETLSG
Sbjct: 341 QSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIETLSG 400

Query: 509 PPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASIMSGREIGSGRTHKSFCAK 568
           PP++E+ + EEEE+R  DE+ K+Q  G  + P+L +A F    SG  +            
Sbjct: 401 PPMLELSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSGSTM------------ 460

Query: 569 SCKLSRALTKNRND-GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLD 628
           + K S  + K  +D GIT+D LHK++ KNVSAWNMKRL+ IVR  S+STLDEQ      +
Sbjct: 461 NMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCE 520

Query: 629 DESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQ 688
           DEST +I+SE+EAKAAA+KIF+NVA  G K+IYL+DL+RF+R DE +KTM LFEGA   +
Sbjct: 521 DESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTK 580

Query: 689 RISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASS 748
           +I+KSALKNW+VNAFRERRALALTLNDTKTAV+KLHHM++ +  I+I+++WLI+L IA+S
Sbjct: 581 KITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATS 640

Query: 749 KFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTT 808
           K+ +FL+SQ+V++AF+FGN+ KT+FE+IIFLF++HP+DVGDR  ID ++MVVEEMNILTT
Sbjct: 641 KYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTT 700

Query: 809 VFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIE 868
           VFLR DNLK++ PN +L  K IHN+ RSPDMG+ +   VHI TP EKI A+K RI SYI+
Sbjct: 701 VFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYID 760

Query: 869 GNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELD 928
              E+WYP   ++ KD++ LN V++A+WL H++NHQ+ GER+ RR++L+EEV+K+  ELD
Sbjct: 761 SKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKILLELD 820

Query: 929 IQYRLLPIDINIRSLPSSAPSIGFPSNWT 952
           IQYR  P+DIN++++P+   S   P  W+
Sbjct: 821 IQYRFHPLDINVKTMPTVVSS-RVPPAWS 836

BLAST of CSPI07G01240 vs. TrEMBL
Match: A0A0A0K619_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G017150 PE=4 SV=1)

HSP 1 Score: 1855.9 bits (4806), Expect = 0.0e+00
Identity = 953/955 (99.79%), Postives = 953/955 (99.79%), Query Frame = 1

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60
           MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60

Query: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG 120
           SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG
Sbjct: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG 120

Query: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRIVPPLAES 180
           VDRNEGFEFVQTGYGMEDPPTKLIGDFL KQKIEGETTLDMDLEMEELKPNRIVPPLAES
Sbjct: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLCKQKIEGETTLDMDLEMEELKPNRIVPPLAES 180

Query: 181 PLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPT 240
           PLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPT
Sbjct: 181 PLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPT 240

Query: 241 ISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSG 300
           ISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSG
Sbjct: 241 ISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSG 300

Query: 301 QLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR 360
           QLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR
Sbjct: 301 QLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR 360

Query: 361 EKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN 420
           EKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN
Sbjct: 361 EKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN 420

Query: 421 CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH 480
           CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH
Sbjct: 421 CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH 480

Query: 481 VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAA 540
           VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAA
Sbjct: 481 VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAA 540

Query: 541 TFASIMSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRL 600
           TFASI SGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRL
Sbjct: 541 TFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRL 600

Query: 601 LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLV 660
           LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLV
Sbjct: 601 LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLV 660

Query: 661 RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM 720
           RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM
Sbjct: 661 RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM 720

Query: 721 VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD 780
           VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD
Sbjct: 721 VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD 780

Query: 781 VGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFL 840
           VGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFL
Sbjct: 781 VGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFL 840

Query: 841 VHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDS 900
           VHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDS
Sbjct: 841 VHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDS 900

Query: 901 GERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 956
           GERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS
Sbjct: 901 GERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 955

BLAST of CSPI07G01240 vs. TrEMBL
Match: A0A0A0KE62_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G124170 PE=4 SV=1)

HSP 1 Score: 1148.3 bits (2969), Expect = 0.0e+00
Identity = 607/942 (64.44%), Postives = 748/942 (79.41%), Query Frame = 1

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60
           MD   KSFK + S+K+ RK+S  GA +  +HE LPIL +HQ   HS        +  SD+
Sbjct: 1   MDFSLKSFKSHPSYKYVRKLS--GADATQDHEHLPILSDHQ---HSI-------MAVSDS 60

Query: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG 120
           SDR EVI+K+D   +S+  +  L    N  G +WRES YDFWN+ +     + R     G
Sbjct: 61  SDRKEVIVKVDEADASTLRATDLV---NGSGTIWRESSYDFWNDSD-----NRRNYGEGG 120

Query: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRI-VPPLAE 180
               + FEF Q    +EDPP+KLIG FL KQK  GE +LDMD+EM EL  ++  +  +AE
Sbjct: 121 AKTTDDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAE 180

Query: 181 SPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSP 240
           SP+ ++S++LKVSF+  S EIS ND S+RRR+RDS  L EE +G Q PRQ          
Sbjct: 181 SPMRRSSRELKVSFESIS-EISEND-SMRRRHRDS-PLDEEHRG-QQPRQCDRRAHG--- 240

Query: 241 TISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKS 300
             S  +++  AE +RC+SN  F  ++SFQRKS+LLRAKTKSRL+DPP   DR SG +PKS
Sbjct: 241 --SNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKS 300

Query: 301 GQLRSGFLGK-IEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPY 360
           GQ+RSG + K ++++DDDPFLE+DLPDE+K+ N   LT+LQW SLI+I AAL+CTL++ Y
Sbjct: 301 GQVRSGLISKALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRY 360

Query: 361 LREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPV 420
            R K LW+L++WKWEVMI +LICGRLVSGWGIR+ VFFIERNFLLRKRVLYFVYGVRK V
Sbjct: 361 WRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAV 420

Query: 421 QNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASS 480
           QNCLWLGLVLIAW+ LF+ +V+++  ++ L YV++VLVCLL+STL+WLVKTLMVKVLASS
Sbjct: 421 QNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASS 480

Query: 481 FHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLK 540
           FHVSTYFDRIQ++LFNQYVIETLSGPPL+EI+KNEEEEER+A+EV KLQNAG  IPPDLK
Sbjct: 481 FHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLK 540

Query: 541 AATFASIMSG-REIGSGRTHKSFCAKSCKLSRALTKNRND-GITIDHLHKLSTKNVSAWN 600
           A  F++   G R IGSG   KS   +S KLSR L+K   D GITIDHLHKLS KNVSAWN
Sbjct: 541 ATAFSTAQKGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWN 600

Query: 601 MKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYL 660
           MKRL+NIVR+G++STLDEQI+    +DESTTEIKSE EAK AAKKIF NVA  G KYIYL
Sbjct: 601 MKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSEYEAKVAAKKIFLNVARNGSKYIYL 660

Query: 661 DDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDK 720
           +DL+RFM +DE  KTM LFEGA E+++ISKS+LKNWVVNAFRERRALALTLNDTKTAV+K
Sbjct: 661 EDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNK 720

Query: 721 LHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVM 780
           LH MVN++  ++IL++WL++LGIA+SKF +F++SQ+V+VAF+FGNTCKT+FE+IIFLFVM
Sbjct: 721 LHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVM 780

Query: 781 HPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGES 840
           HPFDVGDRCEIDG+QM+VEEMNILTT+FLRYDN K+I PNSVLATK IHNFYRSPDMG+ 
Sbjct: 781 HPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDG 840

Query: 841 IEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMN 900
           IEF +HI+TP EKI  M+ RIISYIEG KEHW PAPMIV KD++ LN++++A+WL+HRMN
Sbjct: 841 IEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMN 900

Query: 901 HQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLP 939
           HQD GERW RR++LVEE+VK+ QELD+QYRLLP+DIN+RSLP
Sbjct: 901 HQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLP 913

BLAST of CSPI07G01240 vs. TrEMBL
Match: W9QBV6_9ROSA (Mechanosensitive ion channel protein 8 OS=Morus notabilis GN=L484_006078 PE=4 SV=1)

HSP 1 Score: 1112.1 bits (2875), Expect = 0.0e+00
Identity = 606/981 (61.77%), Postives = 743/981 (75.74%), Query Frame = 1

Query: 3   TLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTD-----HSRCLRDRHPVND 62
           +LKKSFK + S+K++RKISAGG   + +HEELPIL +H   D     H R L D   +  
Sbjct: 4   SLKKSFKSHGSYKNSRKISAGGGNPDNSHEELPILSDHDFDDNHHVQHYRRLNDNGKMGG 63

Query: 63  SDTSDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGD 122
           + T  R EVI+KID GG+  A +        N  N WRE+ Y  W        E+A + D
Sbjct: 64  ASTG-RNEVIVKIDEGGNPPAAA---SDQSRNAENFWRENSYGAWK-------ENANVRD 123

Query: 123 NSGVDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNR----- 182
             G    E F F QT    EDPP+KLI  FL +QK  G+ +LDMDLEMEEL+ +      
Sbjct: 124 GGG--SGESFHFAQT---TEDPPSKLIRQFLHRQKASGDISLDMDLEMEELRRSAGSGGE 183

Query: 183 -IVPPLAESPL-SQTSKDLKVSFQHD----STEISSNDQSIRRRNRDSNDLKEESKGGQS 242
             + P++ESP  S++S++LKVS         +EIS ND ++RRR +DS +  +ES     
Sbjct: 184 LDLTPVSESPQNSRSSRELKVSLHPSPSDVGSEISQND-AVRRRFKDSPNDNDESL---- 243

Query: 243 PRQQPHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPP 302
                  ER G             E +RC SN SF  E+SFQR+S+ LR KT+SRL DPP
Sbjct: 244 ----ERRERDGE------------EVVRCTSNASFQREISFQRRSSFLRVKTRSRLQDPP 303

Query: 303 AEPDRLSGLIPKSGQL------------RSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSA 362
            EPD  SG IPKSGQ+            +SG LGK ++++DDPF ++DLPDEFK+ N SA
Sbjct: 304 EEPDMRSGRIPKSGQIPRSGPIPRSGPMKSGLLGK-DEEEDDPFSDEDLPDEFKKANLSA 363

Query: 363 LTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGV 422
           LT+LQW SLI+I     CTLS+ YLR K+L +L +WKWEV+I +LICGRLVSGWGIRI V
Sbjct: 364 LTLLQWASLILIVGLFACTLSIHYLRHKNLLKLKLWKWEVLILVLICGRLVSGWGIRIAV 423

Query: 423 FFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRV 482
           FF ERNFLLRKRVLYFVYGVRK VQNCLWLGLVLIAWH LF+KRVE++T +  L YV++V
Sbjct: 424 FFFERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWHFLFDKRVERETRSESLKYVTKV 483

Query: 483 LVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEE 542
           L+C L+ TL+WLVKTL+VKVLASSFHVSTYFDRIQ+SLFNQ+VIETLSGPPL+EI K EE
Sbjct: 484 LICFLVGTLLWLVKTLIVKVLASSFHVSTYFDRIQDSLFNQFVIETLSGPPLIEIYKTEE 543

Query: 543 EEERIADEVQKLQNAGIIIPPDLKAATFASIMSGREIGSGRTHKSFCAKSCKLSRALTKN 602
           EEER+ADEV+KLQNAG  IPPDL A + A   + R IGSGR  +++  KS KLSR+L+K 
Sbjct: 544 EEERLADEVEKLQNAGATIPPDL-AFSSALPRTSRVIGSGRLSRTW--KSSKLSRSLSKI 603

Query: 603 RNDGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSERE 662
            ++GITIDHLHKL+ KNVSAWNMKRL+ +VR+G+++TLDEQI     +DES T+I+SE E
Sbjct: 604 GDEGITIDHLHKLNPKNVSAWNMKRLMRMVRHGTLTTLDEQIVDSTHEDESATQIRSEVE 663

Query: 663 AKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVV 722
           AKAAAKKIFQNVA RG K+I L+DL+RFMREDE +KTMSLFEGA+E+QRISKS+LKNWVV
Sbjct: 664 AKAAAKKIFQNVARRGSKFICLEDLMRFMREDEAVKTMSLFEGASESQRISKSSLKNWVV 723

Query: 723 NAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVV 782
           NAFRERRALALTLNDTKTAV+KLH +VNVI  I+I ++WL++LGIA+SKF +F+SSQ+V+
Sbjct: 724 NAFRERRALALTLNDTKTAVNKLHRIVNVIVAIVIGVIWLLILGIATSKFLLFISSQLVL 783

Query: 783 VAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVII 842
           VAFIFGNTCKT+FEAI+FLFVMHPFDVGDRCEIDG+QMVVEEMNILTTVFLRYDN K+I 
Sbjct: 784 VAFIFGNTCKTVFEAIVFLFVMHPFDVGDRCEIDGVQMVVEEMNILTTVFLRYDNAKIIF 843

Query: 843 PNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMI 902
           PNSVLATK I NFYRSPDMG+ IEF +H+ATPA+KI AM+ RI +Y+E  KEHWYPAPM+
Sbjct: 844 PNSVLATKAIDNFYRSPDMGDGIEFSIHVATPADKIAAMRQRITNYVENKKEHWYPAPMV 903

Query: 903 VFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINI 953
           + KD+D LN+V++AVWL HR+NHQD GER+ARRS+L+EE+VK+ QELDIQYRLLPIDIN+
Sbjct: 904 IMKDLDELNRVRMAVWLCHRINHQDMGERYARRSLLIEEMVKIFQELDIQYRLLPIDINV 943

BLAST of CSPI07G01240 vs. TrEMBL
Match: M5VK71_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001020mg PE=4 SV=1)

HSP 1 Score: 1079.7 bits (2791), Expect = 0.0e+00
Identity = 595/990 (60.10%), Postives = 730/990 (73.74%), Query Frame = 1

Query: 3   TLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDTSD 62
           ++KKSFK + S KH RKISAGG    +  E+LPILL+H +       R R P++  D+SD
Sbjct: 4   SIKKSFKSHGSAKHMRKISAGGDDPSL--EQLPILLDHDS-------RHRQPMSPVDSSD 63

Query: 63  RTEVILKIDGG-GSSSAVSRSLDSA--GNNGGNVWRESRYDFWNNDEIGIGESARMGDNS 122
           R EVI+KID G  SSSA +R   +A    NGG +WRES  DFWN D              
Sbjct: 64  RREVIVKIDDGESSSSATTRDAMAADPAKNGGKIWRESSVDFWNED-------------- 123

Query: 123 GVDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPN-RIVPPLA 182
           GV   +GF+F Q     EDPP+KLIG FL KQ+  G+ +LDMDLEMEEL+ N R +PP+A
Sbjct: 124 GVKNGQGFDFAQRRKTAEDPPSKLIGQFLHKQRASGDMSLDMDLEMEELRQNERDLPPVA 183

Query: 183 ESPL-SQTSKDLKVSFQHDSTE-ISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERL 242
           ESP  S+ SK+LKVSFQ  + + + + ++S+RRR RDS                P  ER 
Sbjct: 184 ESPRNSRVSKELKVSFQAPAPDSVETPNESVRRRYRDS----------------PDDERR 243

Query: 243 GSPTISGVQNESLAEAMRCASNLSFHSELSFQRK--SNLLRAKTKSRLIDPPAEPDRLSG 302
            S  +S  Q++     +RC SN SF  E SF  K  S+LLR KT+SRL+DPP EPD  SG
Sbjct: 244 RSGKLSDGQDD----VVRCTSNASFRREPSFANKNRSDLLRIKTRSRLMDPPEEPDFRSG 303

Query: 303 LIPKSGQL------------------RSGFLGKI-EDDDDDPFLEDDLPDEFKRGNFSAL 362
            IP+SGQ+                  +SG LG+  +DDDDDPFLE+D+P E+KR  F+AL
Sbjct: 304 RIPRSGQIPKSGQIPKSGHIPKSGPMKSGMLGRGGDDDDDDPFLEEDVPYEYKRAKFNAL 363

Query: 363 TVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVF 422
           T+LQWVSL++I  ALICTL++P LR KSLW+L +WKWEV+I +LICGRLVSGWGIRI V+
Sbjct: 364 TLLQWVSLVLIVGALICTLTIPVLRLKSLWKLKLWKWEVLILVLICGRLVSGWGIRIIVY 423

Query: 423 FIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVL 482
           F+ERNFLLRKRVLYFVYGVR+ VQNCLWLGLVLIAWH +F+K+VE++T +  L YV++VL
Sbjct: 424 FVERNFLLRKRVLYFVYGVRRAVQNCLWLGLVLIAWHFMFDKKVERETKSEALAYVTKVL 483

Query: 483 VCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEE 542
            CLLI  L+WLVKTL+VKVLASSFHV +YFDRIQ+SLFNQYVIETLSG PL+E++  +EE
Sbjct: 484 FCLLIGVLLWLVKTLIVKVLASSFHVRSYFDRIQDSLFNQYVIETLSGRPLIEMQIEDEE 543

Query: 543 EERIADEVQKLQNAGIIIPPDLKAATFASIMSGREIGSGRTHKS-------FCAKSCKLS 602
           EER+ADEV+KLQNAG  +PPDLKA  F S   G+ IGSG               KS K S
Sbjct: 544 EERLADEVRKLQNAGATMPPDLKANAFPSARIGKVIGSGSLRSGRVIASGGLIGKSTKFS 603

Query: 603 RALTKNRND-GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTT 662
           R L+K   + GITIDHLHKL+ KNVSAWNMKRL+N+VR G ++TLDEQI           
Sbjct: 604 RPLSKKSEETGITIDHLHKLNPKNVSAWNMKRLINMVRKGHLTTLDEQI----------- 663

Query: 663 EIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKS 722
              SE EAKAAAKKIFQNVA RG KYIYL+DL+ FM EDE +KTMSLFEGAAEN+RISKS
Sbjct: 664 ---SEVEAKAAAKKIFQNVARRGSKYIYLEDLMCFMEEDEAVKTMSLFEGAAENRRISKS 723

Query: 723 ALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIF 782
           +LKNWVV+AFRERRALALTLNDTKTAV+ LH MVN+I  I I+++WLIV+GIA++KF +F
Sbjct: 724 SLKNWVVSAFRERRALALTLNDTKTAVNTLHRMVNIIVAIAIVVIWLIVMGIATTKFLLF 783

Query: 783 LSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRY 842
           +SSQ+VVVAF+FGNTCKTIFEA+IFLFVMHPFDVGDRCEI+G+QMVVEEMNILTTVFLRY
Sbjct: 784 VSSQLVVVAFVFGNTCKTIFEAMIFLFVMHPFDVGDRCEINGVQMVVEEMNILTTVFLRY 843

Query: 843 DNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEH 902
           DN K+  PNS+LAT  I NFYRSPD G++IEF +HI+TP +KI  M+ RI+S+IE  KEH
Sbjct: 844 DNTKITYPNSILATLPIFNFYRSPDTGDAIEFSIHISTPPDKIVMMRQRIVSFIEDKKEH 903

Query: 903 WYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRL 956
           WYP PMI+ KD++ LN++K AVW +HRMN QD GERW RR+ +VEE+V++ QELDIQYRL
Sbjct: 904 WYPGPMIIMKDVEELNRIKFAVWPTHRMNFQDMGERWVRRAYVVEEMVRIFQELDIQYRL 933

BLAST of CSPI07G01240 vs. TrEMBL
Match: K7LLZ5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_10G284300 PE=4 SV=1)

HSP 1 Score: 1075.1 bits (2779), Expect = 0.0e+00
Identity = 585/968 (60.43%), Postives = 732/968 (75.62%), Query Frame = 1

Query: 1   MDTLKKSFKGNVSF-KHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSD 60
           M +++KSFK   S+ KH+R   AG    E  HE+LPILL+ QT   S       P  D  
Sbjct: 1   MQSIRKSFKSYGSYNKHSRFFGAGNTDPE--HEQLPILLDQQTLRQSAM-----PAGD-- 60

Query: 61  TSDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNS 120
                  ++KI+  GS +               +WRES Y+FWNND      +  +  +S
Sbjct: 61  ------YVVKINEDGSEAP----------QDNKIWRESSYEFWNNDTTTTTTTTTIPGSS 120

Query: 121 GVDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELK-------PNR 180
                E F+F  +    EDPP++LIG FL KQ+  GE  LDMDLEMEEL+        + 
Sbjct: 121 ----EESFDFRHS----EDPPSQLIGRFLHKQRASGEMQLDMDLEMEELQREGGDDDDDG 180

Query: 181 IVPPLAESPLS-QTSKDLKVSFQHDSTEIS----SNDQSIRRRNRDSNDLKEESKGGQSP 240
            + P+ ESP++ + S++LKVSF+  +  ++     ND   RR ++DS  L E     Q P
Sbjct: 181 KLTPVEESPMTHRVSRELKVSFEEPAYNVNFLETQNDAVRRRHSKDSPSLAEF----QRP 240

Query: 241 RQQPHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPA 300
            Q P ++R  SP+ S    +   E +RC SN SF   LS QRKS LL+AKT+SRL+DPP 
Sbjct: 241 PQPPQYDRRRSPSPSPACGD---EVVRCTSNASFERNLSMQRKSALLKAKTRSRLMDPPE 300

Query: 301 EPDRLSGLIPKSGQLRSGFLGKIEDD-DDDPFLEDDLPDEFKRGNFSALTVLQWVSLIII 360
           EPDR SG + KSGQL SGFLGK  D+ DDDPFLE+DLPDEFK  +FS   +L+W+SLI I
Sbjct: 301 EPDRKSGRVLKSGQLLSGFLGKKNDEEDDDPFLEEDLPDEFKETHFSLWILLEWLSLISI 360

Query: 361 TAALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKR 420
              LI TL VP+LR K+LW+L +WKWEVM+ +LICGRLVS W +RI VF IERNFLLRKR
Sbjct: 361 IGLLITTLCVPFLRNKNLWQLRLWKWEVMVLVLICGRLVSDWVVRIAVFCIERNFLLRKR 420

Query: 421 VLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWL 480
           VLYFVYGVRK VQNC+WLGLVLIAWHLLF+KRV+++T++  L YV++VLVC L+ TL+WL
Sbjct: 421 VLYFVYGVRKAVQNCVWLGLVLIAWHLLFDKRVQRETHSDFLEYVTKVLVCFLVGTLVWL 480

Query: 481 VKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKL 540
           +KTLMVKVLASSFHVSTYFDRIQESLFNQ+VIETLSGPPLVEI+K EEEEER+ADEVQKL
Sbjct: 481 LKTLMVKVLASSFHVSTYFDRIQESLFNQFVIETLSGPPLVEIQKAEEEEERLADEVQKL 540

Query: 541 QNAGIIIPPDLKAATFASIMSGREIGSGRTHKSFCAKSCKLSRALTKNRNDG--ITIDHL 600
           QNAG+ IPPDL+A+ F++I SGR + SG   KS   KS K SR L+K  ++G  IT+D+L
Sbjct: 541 QNAGVTIPPDLRASAFSNIKSGR-LRSGMLQKSPRVKSGKFSRPLSKKSDEGNVITMDNL 600

Query: 601 HKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQ 660
           HKL+  N+SAWNMKRL+N+VR+G++STLDEQI     DD++ T+I+SE EAKAAAKKIF 
Sbjct: 601 HKLNPNNISAWNMKRLMNMVRHGALSTLDEQILDNSNDDDNATQIRSEYEAKAAAKKIFH 660

Query: 661 NVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALA 720
           NVA RG +YIY DDL+RFMREDE  KTM+LFEGA+E  +ISKSALKNWVVNAFRERRALA
Sbjct: 661 NVARRGCRYIYPDDLMRFMREDEAAKTMNLFEGASEAGKISKSALKNWVVNAFRERRALA 720

Query: 721 LTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCK 780
           LTLNDTKTAV+KLH M+N I GI+IL++WL++L +A++KF +F+SSQ+VVVAFIFGNTCK
Sbjct: 721 LTLNDTKTAVNKLHRMLNFIVGIIILVIWLLILELATTKFLLFVSSQVVVVAFIFGNTCK 780

Query: 781 TIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLI 840
           TIFEAIIFLFVMHPFDVGDRCEIDG+QMVVEEMNILTT+FLR+DN KVIIPN+VLATK I
Sbjct: 781 TIFEAIIFLFVMHPFDVGDRCEIDGVQMVVEEMNILTTIFLRFDNQKVIIPNNVLATKAI 840

Query: 841 HNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNK 900
           +N+YRSPDMG++IEF VHI+TP EKI+ +KHRI SYI+  KEHWYP+P+IV++D D LN 
Sbjct: 841 YNYYRSPDMGDAIEFCVHISTPVEKISLIKHRIQSYIDNKKEHWYPSPLIVYRDYDQLNM 900

Query: 901 VKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSI 953
           V+LA+W +HRMN QD GER+ RRS+L+EE++K+ +ELDI YRLLP+DIN+R+ P+++  +
Sbjct: 901 VRLAIWPTHRMNFQDMGERFVRRSLLLEEMIKIFRELDINYRLLPMDINVRATPTTSDRL 925

BLAST of CSPI07G01240 vs. TAIR10
Match: AT1G78610.1 (AT1G78610.1 mechanosensitive channel of small conductance-like 6)

HSP 1 Score: 911.0 bits (2353), Expect = 6.2e-265
Identity = 503/910 (55.27%), Postives = 636/910 (69.89%), Query Frame = 1

Query: 59  DTSDRTEVILKIDG-GGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIG------- 118
           D +DR EVI+KIDG  G+++ VS      G   G +WR+  YDFW + E  +        
Sbjct: 4   DAADRREVIVKIDGENGNNNGVS------GETVGKIWRDGSYDFWTDGEGNLNKGHNAAA 63

Query: 119 -ESARMGDNSGVD-RNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEEL 178
            +S R    +G   ++EGFEF +     EDPPTKLIG FL KQ+  GE  LDMDL M+EL
Sbjct: 64  VDSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLGMDEL 123

Query: 179 KPNRIVPPLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPR 238
           + +R + P++ESP   T +D           +   D      N D  ++ + S G  +P 
Sbjct: 124 Q-SRGLTPVSESPRVSTKRD----------PVGRRDSRSNTNNNDDGEVVKCS-GNNAPI 183

Query: 239 QQPHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAE 298
           Q               ++ S    MR  S LS         ++    A  KS  I  P  
Sbjct: 184 Q---------------RSSSTLLKMRTRSRLSDPPTPQLPPQT----ADMKSGRI--PKS 243

Query: 299 PDRLSGLIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITA 358
               SG   KS + +       E+++DDPF  +DLP+E+++   S   VL+W+SLI+I A
Sbjct: 244 GQMKSGFFGKSPKTQG------EEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSLILIIA 303

Query: 359 ALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVL 418
             +CTL++P LR+K LWEL +WKWE M+ +LICGRLVS W ++I VFFIERNFLLRKRVL
Sbjct: 304 GFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVL 363

Query: 419 YFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVK 478
           YFVYGVRK VQNCLWLGLVL+AWH LF+++V K  NT  L  V+++ VCLL+  L+WLVK
Sbjct: 364 YFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCLLVGFLLWLVK 423

Query: 479 TLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQN 538
           TL+VKVLASSFH+STYFDRIQESLF QYVIETLSGPPL+EI+KNEEEEERI+ EV+K QN
Sbjct: 424 TLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEVKKFQN 483

Query: 539 AGIIIPPDLKAATFASIMSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLS 598
            G +            I SG +    +T KS        +       N GITID LHKL+
Sbjct: 484 PGGV-----------EIQSGAQKSPMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLN 543

Query: 599 TKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAH 658
            KNVSAW MKRL+NI+R GS++TLDEQ++ P LDD+   +I+SE EAK AA+KIF NVA 
Sbjct: 544 PKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFEAKLAARKIFHNVAK 603

Query: 659 RGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLN 718
            G K+IY +D++RF+ +DE LKT+SLFEGA+E  RISKS+LKNWVVNAFRERRALALTLN
Sbjct: 604 PGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLN 663

Query: 719 DTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFE 778
           DTKTAV++LH MVN++ GI+IL++WLI+LGI S+KF + +SSQ+VVVAFIFGN CK +FE
Sbjct: 664 DTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFE 723

Query: 779 AIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFY 838
           +II+LFV+HPFDVGDRCEIDG+QMVVEEMNILTTVFLR+DN KV+ PNS+L TK I N+Y
Sbjct: 724 SIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYY 783

Query: 839 RSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLA 898
           RSPDMG+ IEF +HI TPAEKI  +K RI SYIEG K+HWYPAPMIVFKD++ LN V++A
Sbjct: 784 RSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIA 843

Query: 899 VWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAP---SIG 956
           VW +HRMNHQD GE+WARRS LVEE+ K+C+ELDI+YRL P+DIN+R+LP+S     S  
Sbjct: 844 VWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDINVRNLPTSTALPVSDR 853

BLAST of CSPI07G01240 vs. TAIR10
Match: AT3G14810.1 (AT3G14810.1 mechanosensitive channel of small conductance-like 5)

HSP 1 Score: 889.8 bits (2298), Expect = 1.5e-258
Identity = 491/932 (52.68%), Postives = 652/932 (69.96%), Query Frame = 1

Query: 59  DTSDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDN 118
           D++DR + I+ I+G   S AV  +  S+   GGN+W+ES YDFW+ ++   G++ + GD+
Sbjct: 5   DSTDRRDFIVNINGQ-ESGAVGATGSSSNAEGGNIWKESSYDFWDGEK---GKNDKKGDD 64

Query: 119 SGVDRNEGFEFVQTGYG------MEDPPTKLIGDFLRKQKIEG-ETTLDMDLEMEELKPN 178
              D    F F Q G        + DPP+KLIG FL KQ+  G E +LD++L M EL+ N
Sbjct: 65  EDEDGGS-FHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSN 124

Query: 179 RIVPPLAES--------PLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKG 238
               P   S         +S++S  +K   + D+          RR+NR S        G
Sbjct: 125 TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVR--------RRQNRTS-------LG 184

Query: 239 GQSPRQQPHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLI 298
           G S  +               +N   AE ++C S           +K  L R KTKSRL 
Sbjct: 185 GSSDEEG--------------RNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQ 244

Query: 299 DPPA-------EPDRLSGLIPKSGQLRSGFLGKI------------EDDDDDPFLEDDLP 358
           DPP        + +  SG   +SG  +SGFLGK             E++++DPFL++DLP
Sbjct: 245 DPPTPTHPAIDKTEMKSGR--RSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLP 304

Query: 359 DEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGRL 418
           +EFKR   S    L+W+SL++I  +L+C+L++  L+ K+ W+LD+WKWEV + +LICGRL
Sbjct: 305 EEFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRL 364

Query: 419 VSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTN 478
           VS W +RI VF +E+NF  RKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+K+VE++T 
Sbjct: 365 VSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETR 424

Query: 479 TSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGP 538
           ++ L YV+RVLVCLL++ +IWLVKT++VKVLASSFH+STYFDRIQESLF QYVIETLSGP
Sbjct: 425 STALRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGP 484

Query: 539 PLVEIRKNEEEEERIADEVQKLQN-AGIIIPPDLKAATFASIMSGREIGSGRTHKSFCAK 598
           PL+EI++ EEEE+++A++V+ L+  AG  +PP LKA    ++ S  ++G          K
Sbjct: 485 PLMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKA----TVKSFMKVG----------K 544

Query: 599 SCKLSRALTKNRNDG--ITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCL 658
           S  L+R  +K   DG  I ID L +++TKNVSAWNMKRL+NI+  G+ISTLD+ ++    
Sbjct: 545 SPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQ 604

Query: 659 DDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAEN 718
           +DE  T I+SE EAK AA+KIF NV   G +YIYL+D +RF+ E+E  + M+LFEGA+E+
Sbjct: 605 EDEDATHIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASES 664

Query: 719 QRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIAS 778
            +ISKS LKNWVV AFRERRALALTLNDTKTAVD+LH ++NV+ GI+I+I+WL++LGIA+
Sbjct: 665 DKISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIAT 724

Query: 779 SKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILT 838
           ++F + LSSQ+++VAF+FGN+CKTIFEAIIFLFVMHPFDVGDRCEIDG+Q+VVEEMNILT
Sbjct: 725 TRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILT 784

Query: 839 TVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYI 898
           TVFLRYDN K+I PNSVL TK I N+YRSPDMG+++EF VHIATP EKITA+K RI+SY+
Sbjct: 785 TVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYV 844

Query: 899 EGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQEL 951
           +  K++WYPAPMIVF  +D LN VK+AVWL+HRMNHQD GER+ RR +L+EEV K C+EL
Sbjct: 845 DNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCREL 875

BLAST of CSPI07G01240 vs. TAIR10
Match: AT1G53470.1 (AT1G53470.1 mechanosensitive channel of small conductance-like 4)

HSP 1 Score: 849.0 bits (2192), Expect = 2.9e-246
Identity = 462/923 (50.05%), Postives = 640/923 (69.34%), Query Frame = 1

Query: 60  TSDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNS 119
           T  R + +++IDG  +  +               WRES  +FW+ND+          D S
Sbjct: 6   TDQRRDFVVRIDGEDNGDSEK------------FWRESSINFWHNDKSSKPPGGEEDDGS 65

Query: 120 GVDRNEGFEFVQTGYGME---DPPTKLIGDFLRKQKIEG-ETTLDMDLEMEELKPNRIVP 179
                  F+F++         DPP+KLI  FL KQK  G E +LDM+  M EL+ N  VP
Sbjct: 66  -------FDFMRRSSEKSEEPDPPSKLINQFLNKQKASGDEISLDMEANMPELQKNT-VP 125

Query: 180 PLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHER 239
           PL+ + +S ++  +       S    + D   RR+NR +  L    K G S   + +   
Sbjct: 126 PLSSTAVSGSASPVTAPVTA-SYRNGTGDAIRRRQNRVT--LSPSVKDGDSSEDEENR-- 185

Query: 240 LGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAE--PDRLS 299
                + G      +E ++C SN S        R   L++ KT+SRL+DPP    PD +S
Sbjct: 186 -----VDG------SEVVKCTSNRST------MRTKTLMKMKTRSRLMDPPTPTYPDMVS 245

Query: 300 GLIPKSGQLRSGFLG-------------KIEDDDDDPFLEDDLPDEFKRGNFSALTVLQW 359
           G  P+SG L  GF G             K  ++++DPF E+DLP+  ++       +++W
Sbjct: 246 GRTPRSGNLNPGFSGRNTKPGTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEW 305

Query: 360 VSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERN 419
           + LI+I A+LIC+L +PYLR K+LW+L +WKWEVM+ +LICGRLVS W +++ V+F+E N
Sbjct: 306 IFLILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESN 365

Query: 420 FLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLI 479
           FL RK+VLYFVYG+RKPVQNCLWLGLVLIAWH LF+K+VE++  +++L YV++VL+CLL+
Sbjct: 366 FLWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLV 425

Query: 480 STLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIA 539
           + +IWL+KTL+VKVLASSFH+STYFDRIQESLF QYVIETLSGPP +EI     EEE++A
Sbjct: 426 AVIIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEIHI---EEEKVA 485

Query: 540 DEVQKLQNAGIIIPP-DLKAATFASIMSGREIGSGRTHKS--FCAKSCKLSRALTKNRN- 599
           ++V+  +  G  + P   KA +    ++   +GSGR  KS     KS  LSR+ +K    
Sbjct: 486 NDVKTFEIVGRKLSPLGPKAVSSPPQVT---VGSGRLQKSPSRVGKSPVLSRSGSKKEGG 545

Query: 600 -DGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCL-DDESTTEIKSERE 659
            +GI IDHL +++TKNVSAW MK+L+N+++ G++STLDEQI+     +D+  T+I+SE E
Sbjct: 546 EEGIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFE 605

Query: 660 AKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVV 719
           AK AA+KIFQNVA  G +YIY++D +RF+ EDE  + M LFEGA+E  +ISKS LKNWVV
Sbjct: 606 AKLAARKIFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVV 665

Query: 720 NAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVV 779
           NAFRERRALALTLNDTKTAV++LH +V+V+  I+ILI+WL++LGIA++KF + +SSQ+++
Sbjct: 666 NAFRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLL 725

Query: 780 VAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVII 839
           V F+FGN+CKTIFEA+IF+FVMHPFDVGDRCEIDG+QM+VEEMNILTTVFLR+DN K++ 
Sbjct: 726 VVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVY 785

Query: 840 PNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMI 899
           PNS+L TK I N+YRSPDM ++IEF VHIATP EK TA++ RI+SY++  K+HW+P+PMI
Sbjct: 786 PNSLLGTKPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMI 845

Query: 900 VFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINI 956
           VF+D+ GLN VK+A+W +H+MNHQ+ GER+ RR  L+EE+ ++C+ELDI+YRL P++IN+
Sbjct: 846 VFRDMCGLNSVKIAMWPTHKMNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINV 880

BLAST of CSPI07G01240 vs. TAIR10
Match: AT2G17000.1 (AT2G17000.1 Mechanosensitive ion channel family protein)

HSP 1 Score: 780.0 bits (2013), Expect = 1.7e-225
Identity = 398/689 (57.76%), Postives = 531/689 (77.07%), Query Frame = 1

Query: 269 RKSNLL-RAKTKSRLIDPPAEPDR-LSGLIPKSGQLRSGFLGKIEDD---DDDPFLEDDL 328
           RK+ L+ RAK +SRLIDPP E ++  S  I  S QLRSG LG+  DD   +DD   E+D+
Sbjct: 161 RKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSAEEDV 220

Query: 329 PDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGR 388
           P E+++    A+T+LQW+SLI +  AL+ +L +   R  +LW L +WKWEV++ +LICGR
Sbjct: 221 PVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVLICGR 280

Query: 389 LVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQT 448
           LVSG GIRI VFFIERNFLLRKRVLYFVYGV+  VQNCLWLGLVL+AWH LF+K+VEK+T
Sbjct: 281 LVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKVEKET 340

Query: 449 NTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSG 508
            + +L  +S++LVC L+ST++WL+KTL+VKVLASSFHVSTYFDRIQE+LF+ Y+IETLSG
Sbjct: 341 QSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIETLSG 400

Query: 509 PPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASIMSGREIGSGRTHKSFCAK 568
           PP++E+ + EEEE+R  DE+ K+Q  G  + P+L +A F    SG  +            
Sbjct: 401 PPMLELSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSGSTM------------ 460

Query: 569 SCKLSRALTKNRND-GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLD 628
           + K S  + K  +D GIT+D LHK++ KNVSAWNMKRL+ IVR  S+STLDEQ      +
Sbjct: 461 NMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCE 520

Query: 629 DESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQ 688
           DEST +I+SE+EAKAAA+KIF+NVA  G K+IYL+DL+RF+R DE +KTM LFEGA   +
Sbjct: 521 DESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTK 580

Query: 689 RISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASS 748
           +I+KSALKNW+VNAFRERRALALTLNDTKTAV+KLHHM++ +  I+I+++WLI+L IA+S
Sbjct: 581 KITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATS 640

Query: 749 KFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTT 808
           K+ +FL+SQ+V++AF+FGN+ KT+FE+IIFLF++HP+DVGDR  ID ++MVVEEMNILTT
Sbjct: 641 KYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTT 700

Query: 809 VFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIE 868
           VFLR DNLK++ PN +L  K IHN+ RSPDMG+ +   VHI TP EKI A+K RI SYI+
Sbjct: 701 VFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYID 760

Query: 869 GNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELD 928
              E+WYP   ++ KD++ LN V++A+WL H++NHQ+ GER+ RR++L+EEV+K+  ELD
Sbjct: 761 SKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKILLELD 820

Query: 929 IQYRLLPIDINIRSLPSSAPSIGFPSNWT 952
           IQYR  P+DIN++++P+   S   P  W+
Sbjct: 821 IQYRFHPLDINVKTMPTVVSS-RVPPAWS 836

BLAST of CSPI07G01240 vs. TAIR10
Match: AT2G17010.1 (AT2G17010.1 Mechanosensitive ion channel family protein)

HSP 1 Score: 491.1 bits (1263), Expect = 1.5e-138
Identity = 236/366 (64.48%), Postives = 307/366 (83.88%), Query Frame = 1

Query: 586 KLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQN 645
           +++ KN+SAWNMKRL+ IVR  S++TLDEQ+     +DEST +I+SE+EAKAAA+KIF+N
Sbjct: 410 RMNHKNISAWNMKRLMKIVRNVSLTTLDEQMLESTYEDESTRQIRSEKEAKAAARKIFKN 469

Query: 646 VAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALAL 705
           V  RG KYIYL+DL+RF+REDE +KTM LFEGA EN+RISKSALKNW+VNAFRERRALAL
Sbjct: 470 VEQRGAKYIYLEDLMRFLREDEAMKTMGLFEGAPENKRISKSALKNWLVNAFRERRALAL 529

Query: 706 TLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKT 765
           TLNDTKTAV+KLHHM+N++  I+I+++WL++L IASSK  +F+SSQ+V++AFIFGNT KT
Sbjct: 530 TLNDTKTAVNKLHHMINIVTAIVIVVIWLVLLEIASSKVLLFVSSQVVLLAFIFGNTVKT 589

Query: 766 IFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIH 825
           +FE+IIFLF++HP+DVGDRCEID +Q+VVEEMNILTTVFLRYDNLK++ PNS+L  K I+
Sbjct: 590 VFESIIFLFIVHPYDVGDRCEIDSVQLVVEEMNILTTVFLRYDNLKIMYPNSLLWQKSIN 649

Query: 826 NFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKV 885
           N+YRSPDMG++IEF VHI TP EKI+ +K RI +YI+   E+WYP   I+ KD++ L+ V
Sbjct: 650 NYYRSPDMGDAIEFCVHITTPLEKISVIKQRISNYIDNKPEYWYPQAKIIVKDLEDLHIV 709

Query: 886 KLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIG 945
           +LA+W  HR+NHQD  ERW RR+VLVEEV+K+  ELDIQ+R  P+DIN+R++P+   S  
Sbjct: 710 RLAIWPCHRINHQDMAERWTRRAVLVEEVIKILLELDIQHRFYPLDINVRTMPTVVSS-R 769

Query: 946 FPSNWT 952
            P  W+
Sbjct: 770 VPPGWS 774

BLAST of CSPI07G01240 vs. NCBI nr
Match: gi|449454364|ref|XP_004144925.1| (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis sativus])

HSP 1 Score: 1855.9 bits (4806), Expect = 0.0e+00
Identity = 953/955 (99.79%), Postives = 953/955 (99.79%), Query Frame = 1

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60
           MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60

Query: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG 120
           SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG
Sbjct: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG 120

Query: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRIVPPLAES 180
           VDRNEGFEFVQTGYGMEDPPTKLIGDFL KQKIEGETTLDMDLEMEELKPNRIVPPLAES
Sbjct: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLCKQKIEGETTLDMDLEMEELKPNRIVPPLAES 180

Query: 181 PLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPT 240
           PLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPT
Sbjct: 181 PLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPT 240

Query: 241 ISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSG 300
           ISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSG
Sbjct: 241 ISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSG 300

Query: 301 QLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR 360
           QLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR
Sbjct: 301 QLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR 360

Query: 361 EKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN 420
           EKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN
Sbjct: 361 EKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN 420

Query: 421 CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH 480
           CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH
Sbjct: 421 CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH 480

Query: 481 VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAA 540
           VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAA
Sbjct: 481 VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAA 540

Query: 541 TFASIMSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRL 600
           TFASI SGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRL
Sbjct: 541 TFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRL 600

Query: 601 LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLV 660
           LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLV
Sbjct: 601 LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLV 660

Query: 661 RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM 720
           RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM
Sbjct: 661 RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM 720

Query: 721 VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD 780
           VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD
Sbjct: 721 VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD 780

Query: 781 VGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFL 840
           VGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFL
Sbjct: 781 VGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFL 840

Query: 841 VHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDS 900
           VHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDS
Sbjct: 841 VHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDS 900

Query: 901 GERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 956
           GERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS
Sbjct: 901 GERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 955

BLAST of CSPI07G01240 vs. NCBI nr
Match: gi|659094533|ref|XP_008448113.1| (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis melo])

HSP 1 Score: 1694.5 bits (4387), Expect = 0.0e+00
Identity = 887/961 (92.30%), Postives = 901/961 (93.76%), Query Frame = 1

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60
           MDTLKKSFKGNVSFKHTRKISAGG  SEINHEELPILLNHQ+TDH       H VNDSD 
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDH-------HLVNDSDP 60

Query: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESA------R 120
           SDRTEVILKID GGSS AVSRSLDS  NNGG VWRESRYDFWNNDE GIGESA      R
Sbjct: 61  SDRTEVILKIDDGGSS-AVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGAR 120

Query: 121 MGDNSGVDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRIV 180
           M D SG D NEGF+FVQTGYGMEDPPTKLIGDFL KQKI GETTLDMDLEMEELKPNRI+
Sbjct: 121 MSD-SGDDGNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRII 180

Query: 181 PPLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHE 240
           PPLAESPLSQTSKDLKVSFQ DSTEISSNDQS+RRR RDS+DLKEE KG Q P QQ HHE
Sbjct: 181 PPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHE 240

Query: 241 RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSG 300
           RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRL DPPAEPDRLSG
Sbjct: 241 RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSG 300

Query: 301 LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTL 360
           LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPD+FKRGNF+ALTVLQW SLI+ITAA ICTL
Sbjct: 301 LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTL 360

Query: 361 SVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGV 420
           SVPYLREKSLWELDIWKWEVMI ILICGRLVSGWGIRI VFFIERNFLLRKRVLYFVYGV
Sbjct: 361 SVPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGV 420

Query: 421 RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV 480
           RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV
Sbjct: 421 RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV 480

Query: 481 LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIP 540
           LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAG+ IP
Sbjct: 481 LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIP 540

Query: 541 PDLKAATFASIMSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSA 600
           PDLK ATFASI SGR I S RTHKSFCAKS K SRALTKN NDGITIDHLHKLS KNVSA
Sbjct: 541 PDLK-ATFASIKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSA 600

Query: 601 WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYI 660
           WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVA RGYKYI
Sbjct: 601 WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYI 660

Query: 661 YLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAV 720
           YLDDL+RFMREDEVLKT SLFEGAAEN+RISKS LKNWVVN FRERRALALTLNDTKTAV
Sbjct: 661 YLDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAV 720

Query: 721 DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF 780
           DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF
Sbjct: 721 DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF 780

Query: 781 VMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMG 840
           VMHPFDVGDRCEIDG QMVVEEMNILTTVFLRYDNLK+IIPNSVLATKLIHNFYRSPDMG
Sbjct: 781 VMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMG 840

Query: 841 ESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHR 900
           ES+EF VHIATPAEKITAMK RIISYIEGNKEHW PAPMIVFKDIDGLNK+KLAVWLSHR
Sbjct: 841 ESVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHR 900

Query: 901 MNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA 956
           MNHQDS ERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA
Sbjct: 901 MNHQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA 951

BLAST of CSPI07G01240 vs. NCBI nr
Match: gi|659095000|ref|XP_008448342.1| (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis melo])

HSP 1 Score: 1150.6 bits (2975), Expect = 0.0e+00
Identity = 610/951 (64.14%), Postives = 750/951 (78.86%), Query Frame = 1

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60
           MD   KSFK + S+K+ RK+S  GA +  + E LPIL +HQ   HS        +  SD 
Sbjct: 1   MDFSLKSFKSHPSYKYVRKLS--GADATQDQEHLPILSDHQ---HSI-------MAVSDA 60

Query: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG 120
           SDR EVI+K+D   +SS  +  L    N  G +WRES YDFWN+ +     + R     G
Sbjct: 61  SDRKEVIVKVDEADASSLRATDLV---NGSGRIWRESSYDFWNDSD-----NRRNNGEGG 120

Query: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRI-VPPLAE 180
               E FEF Q    +EDPP+KLIG FL KQK  GE +LDMD+EM EL  ++  +  +AE
Sbjct: 121 ARTTEDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAE 180

Query: 181 SPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSP 240
           SP+ ++S++LKVSF+  S EIS ND S+RRR+RDS  L EE +G Q PRQ          
Sbjct: 181 SPMRRSSRELKVSFESIS-EISEND-SMRRRHRDS-PLDEEHRG-QQPRQCDRRAHG--- 240

Query: 241 TISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKS 300
             S  +++  AE +RC+SN SF  ++SFQRKS+LLRAKTKSRL+DPP   DR SG +PKS
Sbjct: 241 --SNGEDDGAAEVLRCSSNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKS 300

Query: 301 GQLRSGFLGKI-EDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPY 360
           GQ+RSG + K+ +++DDDPFLE+DLPDE+K+ N   LT+LQW SLI+I AAL+CTL++ Y
Sbjct: 301 GQVRSGLISKVLDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRY 360

Query: 361 LREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPV 420
            R K LW+L++WKWEVMI +LICGRLVSGWGIR+ VFFIERNFLLRKRVLYFVYGVRK V
Sbjct: 361 WRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAV 420

Query: 421 QNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASS 480
           QNCLWLGLVLIAW+ LF+ +V+++  ++ L YV++VLVCLLISTL+WLVKTLMVKVLASS
Sbjct: 421 QNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLISTLVWLVKTLMVKVLASS 480

Query: 481 FHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLK 540
           FHVSTYFDRIQ++LFNQYVIETLSGPPL+EI+KNEEEEER+A+EV KLQNAG  IPPDLK
Sbjct: 481 FHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVMKLQNAGATIPPDLK 540

Query: 541 AATFASIMSG-REIGSGRTHKSFCAKSCKLSRALTKNRND-GITIDHLHKLSTKNVSAWN 600
           A  F++   G R IGSG   KS   +S KLSR L+K   D GITIDHLHKLS KNVSAWN
Sbjct: 541 ATAFSTAQKGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWN 600

Query: 601 MKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYL 660
           MKRL+NIVR+G++STLDEQI+    +DESTT+IKSE EAK AAKKIF NVA  G KYIYL
Sbjct: 601 MKRLMNIVRHGTLSTLDEQIKDTAHEDESTTQIKSEYEAKVAAKKIFLNVARNGSKYIYL 660

Query: 661 DDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDK 720
           +DL+RFM +DE  KTM LFEGA E+++ISKS+LKNWVVNAFRERRALALTLNDTKTAV+K
Sbjct: 661 EDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNK 720

Query: 721 LHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVM 780
           LH MVN++  ++IL++WL++LGIA+SKF +F++SQ+V+VAF+FGNTCKT+FE+IIFLFVM
Sbjct: 721 LHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVM 780

Query: 781 HPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGES 840
           HPFDVGDRCEIDG+QM+VEEMNILTT+FLRYDN K+I PNSVLATK IHNFYRSPDMG+ 
Sbjct: 781 HPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDG 840

Query: 841 IEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMN 900
           +EF +HI+TP EKI  M+ RI+SYIEG KEHW PAPMIV KD++ LN++++A+WL+HRMN
Sbjct: 841 VEFCLHISTPPEKIAIMRQRIVSYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMN 900

Query: 901 HQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFP 948
           HQD GERW RR++LVEE+VK+ QELD+QYRLLP+DIN+RSLP    S  FP
Sbjct: 901 HQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLP-PVNSTNFP 921

BLAST of CSPI07G01240 vs. NCBI nr
Match: gi|449444568|ref|XP_004140046.1| (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis sativus])

HSP 1 Score: 1148.3 bits (2969), Expect = 0.0e+00
Identity = 607/942 (64.44%), Postives = 748/942 (79.41%), Query Frame = 1

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60
           MD   KSFK + S+K+ RK+S  GA +  +HE LPIL +HQ   HS        +  SD+
Sbjct: 1   MDFSLKSFKSHPSYKYVRKLS--GADATQDHEHLPILSDHQ---HSI-------MAVSDS 60

Query: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG 120
           SDR EVI+K+D   +S+  +  L    N  G +WRES YDFWN+ +     + R     G
Sbjct: 61  SDRKEVIVKVDEADASTLRATDLV---NGSGTIWRESSYDFWNDSD-----NRRNYGEGG 120

Query: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRI-VPPLAE 180
               + FEF Q    +EDPP+KLIG FL KQK  GE +LDMD+EM EL  ++  +  +AE
Sbjct: 121 AKTTDDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAE 180

Query: 181 SPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSP 240
           SP+ ++S++LKVSF+  S EIS ND S+RRR+RDS  L EE +G Q PRQ          
Sbjct: 181 SPMRRSSRELKVSFESIS-EISEND-SMRRRHRDS-PLDEEHRG-QQPRQCDRRAHG--- 240

Query: 241 TISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKS 300
             S  +++  AE +RC+SN  F  ++SFQRKS+LLRAKTKSRL+DPP   DR SG +PKS
Sbjct: 241 --SNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKS 300

Query: 301 GQLRSGFLGK-IEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPY 360
           GQ+RSG + K ++++DDDPFLE+DLPDE+K+ N   LT+LQW SLI+I AAL+CTL++ Y
Sbjct: 301 GQVRSGLISKALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRY 360

Query: 361 LREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPV 420
            R K LW+L++WKWEVMI +LICGRLVSGWGIR+ VFFIERNFLLRKRVLYFVYGVRK V
Sbjct: 361 WRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAV 420

Query: 421 QNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASS 480
           QNCLWLGLVLIAW+ LF+ +V+++  ++ L YV++VLVCLL+STL+WLVKTLMVKVLASS
Sbjct: 421 QNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASS 480

Query: 481 FHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLK 540
           FHVSTYFDRIQ++LFNQYVIETLSGPPL+EI+KNEEEEER+A+EV KLQNAG  IPPDLK
Sbjct: 481 FHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLK 540

Query: 541 AATFASIMSG-REIGSGRTHKSFCAKSCKLSRALTKNRND-GITIDHLHKLSTKNVSAWN 600
           A  F++   G R IGSG   KS   +S KLSR L+K   D GITIDHLHKLS KNVSAWN
Sbjct: 541 ATAFSTAQKGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWN 600

Query: 601 MKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYL 660
           MKRL+NIVR+G++STLDEQI+    +DESTTEIKSE EAK AAKKIF NVA  G KYIYL
Sbjct: 601 MKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSEYEAKVAAKKIFLNVARNGSKYIYL 660

Query: 661 DDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDK 720
           +DL+RFM +DE  KTM LFEGA E+++ISKS+LKNWVVNAFRERRALALTLNDTKTAV+K
Sbjct: 661 EDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNK 720

Query: 721 LHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVM 780
           LH MVN++  ++IL++WL++LGIA+SKF +F++SQ+V+VAF+FGNTCKT+FE+IIFLFVM
Sbjct: 721 LHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVM 780

Query: 781 HPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGES 840
           HPFDVGDRCEIDG+QM+VEEMNILTT+FLRYDN K+I PNSVLATK IHNFYRSPDMG+ 
Sbjct: 781 HPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDG 840

Query: 841 IEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMN 900
           IEF +HI+TP EKI  M+ RIISYIEG KEHW PAPMIV KD++ LN++++A+WL+HRMN
Sbjct: 841 IEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMN 900

Query: 901 HQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLP 939
           HQD GERW RR++LVEE+VK+ QELD+QYRLLP+DIN+RSLP
Sbjct: 901 HQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLP 913

BLAST of CSPI07G01240 vs. NCBI nr
Match: gi|703062965|ref|XP_010086848.1| (Mechanosensitive ion channel protein 8 [Morus notabilis])

HSP 1 Score: 1112.1 bits (2875), Expect = 0.0e+00
Identity = 606/981 (61.77%), Postives = 743/981 (75.74%), Query Frame = 1

Query: 3   TLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTD-----HSRCLRDRHPVND 62
           +LKKSFK + S+K++RKISAGG   + +HEELPIL +H   D     H R L D   +  
Sbjct: 4   SLKKSFKSHGSYKNSRKISAGGGNPDNSHEELPILSDHDFDDNHHVQHYRRLNDNGKMGG 63

Query: 63  SDTSDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGD 122
           + T  R EVI+KID GG+  A +        N  N WRE+ Y  W        E+A + D
Sbjct: 64  ASTG-RNEVIVKIDEGGNPPAAA---SDQSRNAENFWRENSYGAWK-------ENANVRD 123

Query: 123 NSGVDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNR----- 182
             G    E F F QT    EDPP+KLI  FL +QK  G+ +LDMDLEMEEL+ +      
Sbjct: 124 GGG--SGESFHFAQT---TEDPPSKLIRQFLHRQKASGDISLDMDLEMEELRRSAGSGGE 183

Query: 183 -IVPPLAESPL-SQTSKDLKVSFQHD----STEISSNDQSIRRRNRDSNDLKEESKGGQS 242
             + P++ESP  S++S++LKVS         +EIS ND ++RRR +DS +  +ES     
Sbjct: 184 LDLTPVSESPQNSRSSRELKVSLHPSPSDVGSEISQND-AVRRRFKDSPNDNDESL---- 243

Query: 243 PRQQPHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPP 302
                  ER G             E +RC SN SF  E+SFQR+S+ LR KT+SRL DPP
Sbjct: 244 ----ERRERDGE------------EVVRCTSNASFQREISFQRRSSFLRVKTRSRLQDPP 303

Query: 303 AEPDRLSGLIPKSGQL------------RSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSA 362
            EPD  SG IPKSGQ+            +SG LGK ++++DDPF ++DLPDEFK+ N SA
Sbjct: 304 EEPDMRSGRIPKSGQIPRSGPIPRSGPMKSGLLGK-DEEEDDPFSDEDLPDEFKKANLSA 363

Query: 363 LTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGV 422
           LT+LQW SLI+I     CTLS+ YLR K+L +L +WKWEV+I +LICGRLVSGWGIRI V
Sbjct: 364 LTLLQWASLILIVGLFACTLSIHYLRHKNLLKLKLWKWEVLILVLICGRLVSGWGIRIAV 423

Query: 423 FFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRV 482
           FF ERNFLLRKRVLYFVYGVRK VQNCLWLGLVLIAWH LF+KRVE++T +  L YV++V
Sbjct: 424 FFFERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWHFLFDKRVERETRSESLKYVTKV 483

Query: 483 LVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEE 542
           L+C L+ TL+WLVKTL+VKVLASSFHVSTYFDRIQ+SLFNQ+VIETLSGPPL+EI K EE
Sbjct: 484 LICFLVGTLLWLVKTLIVKVLASSFHVSTYFDRIQDSLFNQFVIETLSGPPLIEIYKTEE 543

Query: 543 EEERIADEVQKLQNAGIIIPPDLKAATFASIMSGREIGSGRTHKSFCAKSCKLSRALTKN 602
           EEER+ADEV+KLQNAG  IPPDL A + A   + R IGSGR  +++  KS KLSR+L+K 
Sbjct: 544 EEERLADEVEKLQNAGATIPPDL-AFSSALPRTSRVIGSGRLSRTW--KSSKLSRSLSKI 603

Query: 603 RNDGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSERE 662
            ++GITIDHLHKL+ KNVSAWNMKRL+ +VR+G+++TLDEQI     +DES T+I+SE E
Sbjct: 604 GDEGITIDHLHKLNPKNVSAWNMKRLMRMVRHGTLTTLDEQIVDSTHEDESATQIRSEVE 663

Query: 663 AKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVV 722
           AKAAAKKIFQNVA RG K+I L+DL+RFMREDE +KTMSLFEGA+E+QRISKS+LKNWVV
Sbjct: 664 AKAAAKKIFQNVARRGSKFICLEDLMRFMREDEAVKTMSLFEGASESQRISKSSLKNWVV 723

Query: 723 NAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVV 782
           NAFRERRALALTLNDTKTAV+KLH +VNVI  I+I ++WL++LGIA+SKF +F+SSQ+V+
Sbjct: 724 NAFRERRALALTLNDTKTAVNKLHRIVNVIVAIVIGVIWLLILGIATSKFLLFISSQLVL 783

Query: 783 VAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVII 842
           VAFIFGNTCKT+FEAI+FLFVMHPFDVGDRCEIDG+QMVVEEMNILTTVFLRYDN K+I 
Sbjct: 784 VAFIFGNTCKTVFEAIVFLFVMHPFDVGDRCEIDGVQMVVEEMNILTTVFLRYDNAKIIF 843

Query: 843 PNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMI 902
           PNSVLATK I NFYRSPDMG+ IEF +H+ATPA+KI AM+ RI +Y+E  KEHWYPAPM+
Sbjct: 844 PNSVLATKAIDNFYRSPDMGDGIEFSIHVATPADKIAAMRQRITNYVENKKEHWYPAPMV 903

Query: 903 VFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINI 953
           + KD+D LN+V++AVWL HR+NHQD GER+ARRS+L+EE+VK+ QELDIQYRLLPIDIN+
Sbjct: 904 IMKDLDELNRVRMAVWLCHRINHQDMGERYARRSLLIEEMVKIFQELDIQYRLLPIDINV 943

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
MSL6_ARATH1.1e-26355.27Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana GN=MSL6 PE=1 SV=1[more]
MSL8_ARATH2.0e-25751.65Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana GN=MSL8 PE=2 SV=2[more]
MSL5_ARATH2.6e-25752.68Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana GN=MSL5 PE=2 SV=1[more]
MSL4_ARATH5.1e-24550.05Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana GN=MSL4 PE=3 SV=1[more]
MSL7_ARATH2.9e-22457.76Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana GN=MSL7 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K619_CUCSA0.0e+0099.79Uncharacterized protein OS=Cucumis sativus GN=Csa_7G017150 PE=4 SV=1[more]
A0A0A0KE62_CUCSA0.0e+0064.44Uncharacterized protein OS=Cucumis sativus GN=Csa_6G124170 PE=4 SV=1[more]
W9QBV6_9ROSA0.0e+0061.77Mechanosensitive ion channel protein 8 OS=Morus notabilis GN=L484_006078 PE=4 SV... [more]
M5VK71_PRUPE0.0e+0060.10Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001020mg PE=4 SV=1[more]
K7LLZ5_SOYBN0.0e+0060.43Uncharacterized protein OS=Glycine max GN=GLYMA_10G284300 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G78610.16.2e-26555.27 mechanosensitive channel of small conductance-like 6[more]
AT3G14810.11.5e-25852.68 mechanosensitive channel of small conductance-like 5[more]
AT1G53470.12.9e-24650.05 mechanosensitive channel of small conductance-like 4[more]
AT2G17000.11.7e-22557.76 Mechanosensitive ion channel family protein[more]
AT2G17010.11.5e-13864.48 Mechanosensitive ion channel family protein[more]
Match NameE-valueIdentityDescription
gi|449454364|ref|XP_004144925.1|0.0e+0099.79PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis sativus][more]
gi|659094533|ref|XP_008448113.1|0.0e+0092.30PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis melo][more]
gi|659095000|ref|XP_008448342.1|0.0e+0064.14PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis melo][more]
gi|449444568|ref|XP_004140046.1|0.0e+0064.44PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis sativus][more]
gi|703062965|ref|XP_010086848.1|0.0e+0061.77Mechanosensitive ion channel protein 8 [Morus notabilis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006685MscS_channel
IPR010920LSM_dom_sf
IPR016688MscS-like_plants/fungi
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
Vocabulary: Biological Process
TermDefinition
GO:0055085transmembrane transport
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:0008150 biological_process
cellular_component GO:0016020 membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G01240.1CSPI07G01240.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006685Mechanosensitive ion channel MscSPFAMPF00924MS_channelcoord: 720..924
score: 3.1
IPR010920LSM domainunknownSSF50182Sm-like ribonucleoproteinscoord: 768..827
score: 6.54
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPIRPIRSF017209Memb_At2g17000coord: 51..955
score: 2.0E
NoneNo IPR availableunknownCoilCoilcoord: 509..529
scor
NoneNo IPR availableGENE3DG3DSA:2.30.30.60coord: 778..827
score: 2.9
NoneNo IPR availablePANTHERPTHR31618FAMILY NOT NAMEDcoord: 82..952
score:
NoneNo IPR availablePANTHERPTHR31618:SF1MECHANOSENSITIVE ION CHANNEL PROTEIN 6-RELATEDcoord: 82..952
score:

The following gene(s) are paralogous to this gene:

None