Cucsa.086750 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.086750
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPectinesterase
Locationscaffold00873 : 142353 .. 144530 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GCGGAGCTTGATCCCGCGCTGGCGACTGCAGAAGAAAACGCAACCGTAATTAAGGTAATGAGTGATGGAACGGGAAATTTCAAGACAGTCACTGAAGCTATTGCGAGCGTGCCAGCGGACAACAAGAAACGTGTAGTGATTTGGATTGGAGTAGGCGTTTATAAGGAGAAACTTAAGATCGATAGAAATAAGCCTTTTGTTACTCTCTATGGCTCTGATCCTAAGAATATGCCTAAGTTGACTTTTGATGGCGATGCTGCTAAATACGGTACCGTTTATAGTGCTACATTGATTGTTGAAGCGGATTACTTCACTGCGGCTAATCTGATCATTGAGGTATAATTTGTTCATTATTAAATATGTTTTAAGGTTTGATTAGCAAAATGGTTCCTATAATTCGAAAAAAATTAGAATTTAGTTCTTTTGGTTTGATCCGATCGTTGTCACAACAACAATTTTGATAGTAGAGATTATCTATGTTATTTTTTTACAAATCAAAATCTAAGAACTATTTATCATATTTTATTGATGACCATTTATCATATTTTATGAATTTAATCCATTAAAAATTATCAGGCACGATTTAAATTTATTTCTAACTAACTATTTATTTGAATCCTTATGTATGATAGATTATCATAATTCTTTATCCAAATGGTTGAATTCTTAATTTAAAAAAATTAAATCTTTTTAAGTTTTCTGAAGTTGAAAATTATAACAAATAACACCTAGAAAAACAATATAAAAACAACTTAAAATTATTTAAAAGAAATTAAATCCGTAAATTTTTTAGAATTTTATGATTATTAATGAATAGCAACCATCAACCATAACCACATTTTTTCAAATTAAAAATTATTACTAGCTATGGAAGGTAACTATTATATATATATATATATATATATATATATATATTCTATTATTTATTTATTGTTGTATTATATATTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGTATATATATATATATATATATATATATATATATATATTTCAATTATTTTATTTTATGGTTGTATTATATATTGTGTTATCTAGGTTATATATTTGTTGAAAAATGATATGAACAGAACTCATCACCAAGGCCAGATGGGGTAAGAAAAGGAGCACAAGCATTAGCGGCAAGATTTATGGGAACTAAAGCCGCAATTTACAATTGCAAATTCCTTGGATTTCAAGATACCCTTTGTGACGATGACGGTTTACATTTCTACAAAGATTGCTTCATTCAAGGCACTGTTGATTTCATTTTCGGAAAAGGAACTTCCCTTTATTTGGTATAATTTAATTATCTTATTAACGTAACGTTGTTGTTGTTTATTATTATTCTTATATAAATATTTAAATGAATATTCTAAATACTTAATTACAGAACACCCAATTGGACGTGGCTGGAGATGGAGGATTGGCAGTGATAACAGCCCATTCAAGAGAACAAGAAGCTGACACCAGTGGTTATTCGTTTGTTCATTGTAGCATTACTGGAACTGGCGGTAAAAACACTTACTTGGGAAGAGCTTGGATGCCAAGATCTAGAGTTGTCTTTGCCTACACTACAATAGCTGATATCATTCATCCCGAAGGCTGGAATGACATGAAGCATGCAGGTTTCGACAAGTAAGTAGTAACTCTCAAATTCTTTTTACCTTTTTTCAGACTAAGATTACATTATGCTTAGTAATGACATGAAGCATGCTAGTTTTACTATGTTTGTTATTCTTAAAAGTTATACTATTATGATCAAAATTATAATTAAAAGTTAGATTCAAAATGCATGGGGTCAAATTGAATGCTTTTTTTTCTTGTATTTTTGCAGGACGGTTATGTTCGGAGAATATAAGTGTTCAGGGCCAGGAGCTGTGTCGACCGGACGTGTAGCCTACGGCAAGCAATTGACTGATGTAGAAGTGAAGCCATTTCTTGGTCTGGAATACGTGCAAAGTGAGAAGTGGTTGCTTCCTCCTCCCAGTCCTCAAGTTTAG

mRNA sequence

GCGGAGCTTGATCCCGCGCTGGCGACTGCAGAAGAAAACGCAACCGTAATTAAGGTAATGAGTGATGGAACGGGAAATTTCAAGACAGTCACTGAAGCTATTGCGAGCGTGCCAGCGGACAACAAGAAACGTGTAGTGATTTGGATTGGAGTAGGCGTTTATAAGGAGAAACTTAAGATCGATAGAAATAAGCCTTTTGTTACTCTCTATGGCTCTGATCCTAAGAATATGCCTAAGTTGACTTTTGATGGCGATGCTGCTAAATACGGTACCGTTTATAGTGCTACATTGATTGTTGAAGCGGATTACTTCACTGCGGCTAATCTGATCATTGAGAACTCATCACCAAGGCCAGATGGGGTAAGAAAAGGAGCACAAGCATTAGCGGCAAGATTTATGGGAACTAAAGCCGCAATTTACAATTGCAAATTCCTTGGATTTCAAGATACCCTTTGTGACGATGACGGTTTACATTTCTACAAAGATTGCTTCATTCAAGGCACTGTTGATTTCATTTTCGGAAAAGGAACTTCCCTTTATTTGAACACCCAATTGGACGTGGCTGGAGATGGAGGATTGGCAGTGATAACAGCCCATTCAAGAGAACAAGAAGCTGACACCAGTGGTTATTCGTTTGTTCATTGTAGCATTACTGGAACTGGCGGTAAAAACACTTACTTGGGAAGAGCTTGGATGCCAAGATCTAGAGTTGTCTTTGCCTACACTACAATAGCTGATATCATTCATCCCGAAGGCTGGAATGACATGAAGCATGCAGGTTTCGACAAGACGGTTATGTTCGGAGAATATAAGTGTTCAGGGCCAGGAGCTGTGTCGACCGGACGTGTAGCCTACGGCAAGCAATTGACTGATGTAGAAGTGAAGCCATTTCTTGGTCTGGAATACGTGCAAAGTGAGAAGTGGTTGCTTCCTCCTCCCAGTCCTCAAGTTTAG

Coding sequence (CDS)

GCGGAGCTTGATCCCGCGCTGGCGACTGCAGAAGAAAACGCAACCGTAATTAAGGTAATGAGTGATGGAACGGGAAATTTCAAGACAGTCACTGAAGCTATTGCGAGCGTGCCAGCGGACAACAAGAAACGTGTAGTGATTTGGATTGGAGTAGGCGTTTATAAGGAGAAACTTAAGATCGATAGAAATAAGCCTTTTGTTACTCTCTATGGCTCTGATCCTAAGAATATGCCTAAGTTGACTTTTGATGGCGATGCTGCTAAATACGGTACCGTTTATAGTGCTACATTGATTGTTGAAGCGGATTACTTCACTGCGGCTAATCTGATCATTGAGAACTCATCACCAAGGCCAGATGGGGTAAGAAAAGGAGCACAAGCATTAGCGGCAAGATTTATGGGAACTAAAGCCGCAATTTACAATTGCAAATTCCTTGGATTTCAAGATACCCTTTGTGACGATGACGGTTTACATTTCTACAAAGATTGCTTCATTCAAGGCACTGTTGATTTCATTTTCGGAAAAGGAACTTCCCTTTATTTGAACACCCAATTGGACGTGGCTGGAGATGGAGGATTGGCAGTGATAACAGCCCATTCAAGAGAACAAGAAGCTGACACCAGTGGTTATTCGTTTGTTCATTGTAGCATTACTGGAACTGGCGGTAAAAACACTTACTTGGGAAGAGCTTGGATGCCAAGATCTAGAGTTGTCTTTGCCTACACTACAATAGCTGATATCATTCATCCCGAAGGCTGGAATGACATGAAGCATGCAGGTTTCGACAAGACGGTTATGTTCGGAGAATATAAGTGTTCAGGGCCAGGAGCTGTGTCGACCGGACGTGTAGCCTACGGCAAGCAATTGACTGATGTAGAAGTGAAGCCATTTCTTGGTCTGGAATACGTGCAAAGTGAGAAGTGGTTGCTTCCTCCTCCCAGTCCTCAAGTTTAG

Protein sequence

AELDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKIDRNKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGLEYVQSEKWLLPPPSPQV*
Homology
[Close]
BLAST of Cucsa.086750 vs. Swiss-Prot
Match: PME63_ARATH (Putative pectinesterase 63 OS=Arabidopsis thaliana GN=PME63 PE=3 SV=2)

HSP 1 Score: 373.6 bits (958), Expect = 2.1e-102
Identity = 179/296 (60.47%), Postives = 223/296 (75.34%), Query Frame = 1 

Query: 19  VMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKIDRNKPFVTLYGSDPKNMP 78
           V  +G G+FKT+TEAI SV A N +RV+I IG GVYKEK+ IDR+KPF+TLYG  P  MP
Sbjct: 45  VKQNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFITLYGH-PNAMP 104

Query: 79  KLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQALAARFMGTKAA 138
            LTFDG AA+YGTV SATLIV +DYF A N+I++NS+P PDG RKGAQAL+ R  G KAA
Sbjct: 105 VLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQALSMRISGNKAA 164

Query: 139 IYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDVAGDGGLAVITA 198
            YNCKF G+QDT+CDD G HF+KDC+I+GT DFIFG G SLYL TQL+V GD G+ VITA
Sbjct: 165 FYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVVGD-GIRVITA 224

Query: 199 HSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADIIHPEGWNDMKH 258
           H+ +  A+ SGYSFVHC +TGT G   YLGR+WM   +VV+AYT ++ +++P GW + + 
Sbjct: 225 HAGKSAAEKSGYSFVHCKVTGT-GTGIYLGRSWMSHPKVVYAYTDMSSVVNPSGWQENRE 284

Query: 259 AGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGLEYVQSEKWLLPPPS 315
           AG DKTV +GEYKC+G G+    RV Y + + D+E K F+ L Y+Q   WLLPPPS
Sbjct: 285 AGRDKTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWLLPPPS 337

[Close]
BLAST of Cucsa.086750 vs. Swiss-Prot
Match: PPME1_ARATH (Pectinesterase PPME1 OS=Arabidopsis thaliana GN=PPME1 PE=1 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.2e-100
Identity = 178/312 (57.05%), Postives = 222/312 (71.15%), Query Frame = 1 

Query: 3   LDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKIDR 62
           LDPAL  AE    +I V   G G FKT+T+AI SVPA N KRV+I +  G YKEK+ IDR
Sbjct: 53  LDPALVAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAPGEYKEKVTIDR 112

Query: 63  NKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVR 122
           NKPF+TL G  P  MP +T+DG AAKYGTV SA+LI+ +DYF A N++++N++P PDG  
Sbjct: 113 NKPFITLMGQ-PNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKT 172

Query: 123 KGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLN 182
           KGAQAL+ R  G  AA YNCKF GFQDT+CDD G HF+KDC+++GT DFIFG GTS+YL 
Sbjct: 173 KGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLG 232

Query: 183 TQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYT 242
           TQL V GD G+ VI AH+ +   + SGYSFVHC +TGTGG   YLGRAWM   +VV+AYT
Sbjct: 233 TQLHVVGD-GIRVIAAHAGKSAEEKSGYSFVHCKVTGTGG-GIYLGRAWMSHPKVVYAYT 292

Query: 243 TIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGLEY 302
            +  +++P GW + K    DKTV +GEYKCSGPG+    RV + + + D E   FL L Y
Sbjct: 293 EMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANRFLSLGY 352

Query: 303 VQSEKWLLPPPS 315
           +Q  KWLLPPP+
Sbjct: 353 IQGSKWLLPPPA 360

[Close]
BLAST of Cucsa.086750 vs. Swiss-Prot
Match: PME48_ARATH (Probable pectinesterase 48 OS=Arabidopsis thaliana GN=PME48 PE=2 SV=2)

HSP 1 Score: 365.9 bits (938), Expect = 4.4e-100
Identity = 177/312 (56.73%), Postives = 222/312 (71.15%), Query Frame = 1 

Query: 3   LDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKIDR 62
           LDPAL  AE    +I V   G G FKT+T+AI SVPA N KRV+I +  G Y+EK+ IDR
Sbjct: 53  LDPALVAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAHGEYREKVTIDR 112

Query: 63  NKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVR 122
           NKPF+TL G  P  MP +T+DG AAKYGTV SA+LI+ +DYF A N++++N++P PDG  
Sbjct: 113 NKPFITLMGQ-PNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKT 172

Query: 123 KGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLN 182
           KGAQAL+ R  G  AA YNCKF GFQDT+CDD G HF+KDC+++GT DFIFG GTS+YL 
Sbjct: 173 KGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLG 232

Query: 183 TQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYT 242
           TQL V GD G+ VI AH+ +   + SGYSFVHC +TGTGG   YLGRAWM   +VV+AYT
Sbjct: 233 TQLHVVGD-GIRVIAAHAGKSAEEKSGYSFVHCKVTGTGG-GIYLGRAWMSHPKVVYAYT 292

Query: 243 TIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGLEY 302
            +  +++P GW + K    DKTV +GEYKCSGPG+    RV + + + D E   FL L Y
Sbjct: 293 EMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLSLGY 352

Query: 303 VQSEKWLLPPPS 315
           +Q  KWLLPPP+
Sbjct: 353 IQGSKWLLPPPA 360

[Close]
BLAST of Cucsa.086750 vs. Swiss-Prot
Match: AL11B_OLEEU (Pectinesterase 2 OS=Olea europaea PE=1 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 1.8e-93
Identity = 172/312 (55.13%), Postives = 217/312 (69.55%), Query Frame = 1 

Query: 1   AELDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKI 60
           A +DPAL TAE  A VIK+ SDG+G+FK++ EAI S+P DN KRV++    G Y EK+KI
Sbjct: 51  ATMDPALVTAEGQAKVIKLKSDGSGDFKSINEAIKSIPDDNTKRVILSFSPGNYSEKVKI 110

Query: 61  DRNKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDG 120
              K ++T YG DP NMP L F G AA+YGTV SATLIVE++YF+A NL I NS+PRPDG
Sbjct: 111 GMYKHYITFYGEDPNNMPILVFGGTAAEYGTVDSATLIVESNYFSAVNLKIVNSAPRPDG 170

Query: 121 VRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLY 180
            R GAQA A R  G KA+ YN K  GFQDTLCDD G HFYKDC+I+GTVDFIFG G S++
Sbjct: 171 KRVGAQAAALRISGDKASFYNVKIYGFQDTLCDDKGKHFYKDCYIEGTVDFIFGSGKSIF 230

Query: 181 LNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFA 240
           LNT+L        A+ITA +R+ E++ +GY FV+C +TG G    +LGR+WMP ++VVFA
Sbjct: 231 LNTELHAVPGDQPAIITAQARKTESEDTGYYFVNCRVTGGG---AFLGRSWMPAAKVVFA 290

Query: 241 YTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGL 300
           YT + D IHPEGW  +K    + TV F EY   GPGA    R  + K+L+D E K  + L
Sbjct: 291 YTEMGDAIHPEGWILVK-PEHESTVRFPEYNNKGPGANMEKRAKFVKRLSDAEAKQSISL 350

Query: 301 EYVQSEKWLLPP 313
             +++ KWLLPP
Sbjct: 351 GSIEASKWLLPP 358

[Close]
BLAST of Cucsa.086750 vs. Swiss-Prot
Match: AL11A_OLEEU (Pectinesterase 1 OS=Olea europaea PE=1 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 4.0e-93
Identity = 170/312 (54.49%), Postives = 217/312 (69.55%), Query Frame = 1 

Query: 1   AELDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKI 60
           A +DPAL TAE    VIK+ SDG+G+FK++ EAI S+P DN KRV++ +  G Y EK+KI
Sbjct: 51  ATMDPALVTAEGQTKVIKLKSDGSGDFKSINEAIKSIPDDNTKRVILSLAPGNYSEKVKI 110

Query: 61  DRNKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDG 120
              K ++T YG DP NMP L F G AA+YGTV SATLIVE++YF+A NL I NS+PRPDG
Sbjct: 111 GMYKHYITFYGEDPNNMPILVFGGTAAEYGTVDSATLIVESNYFSAVNLKIVNSAPRPDG 170

Query: 121 VRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLY 180
            R GAQA A R  G KA+ YN K  GFQDTLCDD G HFYKDC+I+GTVDFIFG G S++
Sbjct: 171 KRVGAQAAALRISGDKASFYNVKIYGFQDTLCDDKGKHFYKDCYIEGTVDFIFGSGKSIF 230

Query: 181 LNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFA 240
           LNT+L        A+ITA +R+ +++ +GY FV+C +TG G    +LGR+WMP ++VVFA
Sbjct: 231 LNTELHAVPGDQPAIITAQARKTDSEDTGYYFVNCRVTGGG---AFLGRSWMPAAKVVFA 290

Query: 241 YTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGL 300
           YT + D IHPEGW  +K    + TV F EY   GPGA    R  + K+L+D E K  + L
Sbjct: 291 YTEMVDAIHPEGWILVK-PEHESTVRFSEYNNKGPGANMEKRAKFVKRLSDAEAKQSISL 350

Query: 301 EYVQSEKWLLPP 313
             +++ KWLLPP
Sbjct: 351 GSIEASKWLLPP 358

[Close]
BLAST of Cucsa.086750 vs. TrEMBL
Match: A0A0A0KEH2_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_6G428030 PE=4 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 2.2e-183
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 1 

Query: 1   AELDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKI 60
           AELDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKI
Sbjct: 54  AELDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKI 113

Query: 61  DRNKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDG 120
           DRNKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDG
Sbjct: 114 DRNKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDG 173

Query: 121 VRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLY 180
           VRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLY
Sbjct: 174 VRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLY 233

Query: 181 LNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFA 240
           LNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFA
Sbjct: 234 LNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFA 293

Query: 241 YTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGL 300
           YTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGL
Sbjct: 294 YTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGL 353

Query: 301 EYVQSEKWLLPPPSPQV 318
           EYVQSEKWLLPPPSPQV
Sbjct: 354 EYVQSEKWLLPPPSPQV 370

[Close]
BLAST of Cucsa.086750 vs. TrEMBL
Match: A0A0A0KI78_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_6G428290 PE=4 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 2.5e-150
Identity = 261/316 (82.59%), Postives = 288/316 (91.14%), Query Frame = 1 

Query: 1   AELDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKI 60
           AELDPA+  AE N T++KVMSDGTG+FKTVTEAIASVP +NK RVVIWIG GVYKEKL I
Sbjct: 55  AELDPAVVAAEGNVTIVKVMSDGTGDFKTVTEAIASVPVNNKNRVVIWIGEGVYKEKLTI 114

Query: 61  DRNKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDG 120
           ++NKPF+TL G+ PKN+P LTFDG A+KYGTVYSATLIVEADYF AANLIIEN+SPRP+G
Sbjct: 115 EKNKPFITLCGT-PKNVPTLTFDGVASKYGTVYSATLIVEADYFVAANLIIENTSPRPNG 174

Query: 121 VRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLY 180
            RK AQALAARF GTK+A YNCKFLGFQDTLCDDDGLH YKDCFIQGTVDF+FGKGTSLY
Sbjct: 175 -RKEAQALAARFRGTKSAFYNCKFLGFQDTLCDDDGLHLYKDCFIQGTVDFVFGKGTSLY 234

Query: 181 LNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFA 240
           LNT+L+V G+G  AVITAHSREQEAD SGYSFVHCSITG G K+T+LGRAWMPRSRV+FA
Sbjct: 235 LNTELNVVGEGQFAVITAHSREQEADASGYSFVHCSITGNG-KDTFLGRAWMPRSRVIFA 294

Query: 241 YTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGL 300
           YT++ DIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLT+ EVKP+L L
Sbjct: 295 YTSMIDIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTEAEVKPYLSL 354

Query: 301 EYVQSEKWLLPPPSPQ 317
           E+VQS KWLLPPP+PQ
Sbjct: 355 EFVQSAKWLLPPPNPQ 367

[Close]
BLAST of Cucsa.086750 vs. TrEMBL
Match: A0A0A0KJZ4_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_6G428040 PE=4 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 9.4e-150
Identity = 259/316 (81.96%), Postives = 287/316 (90.82%), Query Frame = 1 

Query: 1   AELDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKI 60
           AELDPA+  AE N T++KVM DGTG+FKT+TEAIASVP +NK RVVIWIG GVYKEKL I
Sbjct: 26  AELDPAVVAAEGNVTIVKVMGDGTGDFKTITEAIASVPVNNKNRVVIWIGEGVYKEKLTI 85

Query: 61  DRNKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDG 120
           ++NKPF+TL G+ PKN+P L+FDG A+KYGTVYSATLIVEADYF AANLIIEN+SPRP+G
Sbjct: 86  EKNKPFITLCGT-PKNVPTLSFDGVASKYGTVYSATLIVEADYFVAANLIIENTSPRPNG 145

Query: 121 VRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLY 180
            RK AQALAARF GTK+A YNCKFLGFQDTLCDDDGLH YKDCFIQGTVDF+FGKGTSLY
Sbjct: 146 -RKEAQALAARFRGTKSAFYNCKFLGFQDTLCDDDGLHLYKDCFIQGTVDFVFGKGTSLY 205

Query: 181 LNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFA 240
           LNT+L+V G+G  AVITAHSREQEAD SGYSFVHCSITG G K+TYLGRAWMPRSRV+FA
Sbjct: 206 LNTELNVVGEGQFAVITAHSREQEADASGYSFVHCSITGNG-KDTYLGRAWMPRSRVIFA 265

Query: 241 YTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGL 300
           YT++ DIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLT+ EVKP+L L
Sbjct: 266 YTSMIDIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTEAEVKPYLSL 325

Query: 301 EYVQSEKWLLPPPSPQ 317
           E+VQS KWLLPPP+PQ
Sbjct: 326 EFVQSAKWLLPPPNPQ 338

[Close]
BLAST of Cucsa.086750 vs. TrEMBL
Match: A0A0A0KEY2_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_6G428540 PE=4 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 3.6e-141
Identity = 248/313 (79.23%), Postives = 272/313 (86.90%), Query Frame = 1 

Query: 2   ELDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKID 61
           +LD  L  AEENATVIKV  DG+G+FKT+TEAI SVPA N KRVVIWIG GVYKEKLKID
Sbjct: 56  KLDSELVAAEENATVIKVRGDGSGDFKTITEAIESVPACNTKRVVIWIGGGVYKEKLKID 115

Query: 62  RNKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGV 121
           RNKPFVTLYGS P NMP LTFDGDA+KYGTVYSATL VEADYFTAANLIIENSSPRPDG 
Sbjct: 116 RNKPFVTLYGS-PNNMPNLTFDGDASKYGTVYSATLTVEADYFTAANLIIENSSPRPDGK 175

Query: 122 RKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYL 181
           RKG QALAAR  G K AIYNCKF+GFQDTLCDD GLH YKDCFIQGTVDFIFG GTSLYL
Sbjct: 176 RKGEQALAARIWGNKVAIYNCKFIGFQDTLCDDRGLHLYKDCFIQGTVDFIFGGGTSLYL 235

Query: 182 NTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAY 241
           NTQLDV  DGGL VI AHSREQE++ SG+SFVHCSITGTGG+NTYLGRAW PRSRVVFAY
Sbjct: 236 NTQLDVVVDGGLGVIAAHSREQESECSGFSFVHCSITGTGGRNTYLGRAWRPRSRVVFAY 295

Query: 242 TTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGLE 301
           TT+ADIIHP+GWNDM + G+DKTV FGEYKCSG G+  + RV YGKQL+D +V+ F+ LE
Sbjct: 296 TTMADIIHPKGWNDMDNFGYDKTVSFGEYKCSGLGSNFSKRVQYGKQLSDEKVQQFVSLE 355

Query: 302 YVQSEKWLLPPPS 315
           Y++S+ WLLPPP+
Sbjct: 356 YLKSDTWLLPPPN 367

[Close]
BLAST of Cucsa.086750 vs. TrEMBL
Match: M5XH72_PRUPE (Pectinesterase OS=Prunus persica GN=PRUPE_ppa007766mg PE=4 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 2.8e-117
Identity = 206/315 (65.40%), Postives = 246/315 (78.10%), Query Frame = 1 

Query: 3   LDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKIDR 62
           LDPALATAE   TVIKVM DG+G FKT+T+AI S+PADN KRV+++IG G Y+EK+ I R
Sbjct: 44  LDPALATAEAGQTVIKVMKDGSGQFKTITDAINSIPADNTKRVIVYIGEGKYEEKITIPR 103

Query: 63  NKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVR 122
           NKPFVT +GS P NMP LTF G A KYGTV SAT+IVE+DYF AAN+II+NSSPRPDG  
Sbjct: 104 NKPFVTFFGS-PTNMPTLTFAGTAQKYGTVNSATVIVESDYFMAANVIIKNSSPRPDGKA 163

Query: 123 KGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLN 182
            GAQA+A R  G K+A+YNCK +GFQDT CDD G HF KDCFI+GTVDFI+G G SLYLN
Sbjct: 164 VGAQAVALRVSGNKSALYNCKLIGFQDTFCDDKGNHFLKDCFIEGTVDFIWGSGKSLYLN 223

Query: 183 TQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYT 242
            +L V GD GL VITA +R+  +D +GYSFVHC ITGT G  TYLGRAW     VVFAYT
Sbjct: 224 NELHVVGDNGLTVITAQARDSSSDDTGYSFVHCKITGT-GNGTYLGRAWRISPMVVFAYT 283

Query: 243 TIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGLEY 302
           +++++I+P GW+D  H   D TV +GEYKCSGPG+ + GRV Y KQLTD ++KPFL L Y
Sbjct: 284 SMSEVINPAGWSDDNHPERDNTVFYGEYKCSGPGSSAAGRVKYTKQLTDEQIKPFLSLGY 343

Query: 303 VQSEKWLLPPPSPQV 318
           +Q  KWLLPPP+P+V
Sbjct: 344 IQGSKWLLPPPNPKV 356

[Close]
BLAST of Cucsa.086750 vs. TAIR10
Match: AT5G61680.1 (AT5G61680.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 373.6 bits (958), Expect = 1.2e-103
Identity = 179/296 (60.47%), Postives = 223/296 (75.34%), Query Frame = 1 

Query: 19  VMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKIDRNKPFVTLYGSDPKNMP 78
           V  +G G+FKT+TEAI SV A N +RV+I IG GVYKEK+ IDR+KPF+TLYG  P  MP
Sbjct: 45  VKQNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFITLYGH-PNAMP 104

Query: 79  KLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQALAARFMGTKAA 138
            LTFDG AA+YGTV SATLIV +DYF A N+I++NS+P PDG RKGAQAL+ R  G KAA
Sbjct: 105 VLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQALSMRISGNKAA 164

Query: 139 IYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDVAGDGGLAVITA 198
            YNCKF G+QDT+CDD G HF+KDC+I+GT DFIFG G SLYL TQL+V GD G+ VITA
Sbjct: 165 FYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVVGD-GIRVITA 224

Query: 199 HSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADIIHPEGWNDMKH 258
           H+ +  A+ SGYSFVHC +TGT G   YLGR+WM   +VV+AYT ++ +++P GW + + 
Sbjct: 225 HAGKSAAEKSGYSFVHCKVTGT-GTGIYLGRSWMSHPKVVYAYTDMSSVVNPSGWQENRE 284

Query: 259 AGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGLEYVQSEKWLLPPPS 315
           AG DKTV +GEYKC+G G+    RV Y + + D+E K F+ L Y+Q   WLLPPPS
Sbjct: 285 AGRDKTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWLLPPPS 337

[Close]
BLAST of Cucsa.086750 vs. TAIR10
Match: AT1G69940.1 (AT1G69940.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 367.9 bits (943), Expect = 6.5e-102
Identity = 178/312 (57.05%), Postives = 222/312 (71.15%), Query Frame = 1 

Query: 3   LDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKIDR 62
           LDPAL  AE    +I V   G G FKT+T+AI SVPA N KRV+I +  G YKEK+ IDR
Sbjct: 53  LDPALVAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAPGEYKEKVTIDR 112

Query: 63  NKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVR 122
           NKPF+TL G  P  MP +T+DG AAKYGTV SA+LI+ +DYF A N++++N++P PDG  
Sbjct: 113 NKPFITLMGQ-PNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKT 172

Query: 123 KGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLN 182
           KGAQAL+ R  G  AA YNCKF GFQDT+CDD G HF+KDC+++GT DFIFG GTS+YL 
Sbjct: 173 KGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLG 232

Query: 183 TQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYT 242
           TQL V GD G+ VI AH+ +   + SGYSFVHC +TGTGG   YLGRAWM   +VV+AYT
Sbjct: 233 TQLHVVGD-GIRVIAAHAGKSAEEKSGYSFVHCKVTGTGG-GIYLGRAWMSHPKVVYAYT 292

Query: 243 TIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGLEY 302
            +  +++P GW + K    DKTV +GEYKCSGPG+    RV + + + D E   FL L Y
Sbjct: 293 EMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANRFLSLGY 352

Query: 303 VQSEKWLLPPPS 315
           +Q  KWLLPPP+
Sbjct: 353 IQGSKWLLPPPA 360

[Close]
BLAST of Cucsa.086750 vs. TAIR10
Match: AT5G07410.1 (AT5G07410.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 365.9 bits (938), Expect = 2.5e-101
Identity = 177/312 (56.73%), Postives = 222/312 (71.15%), Query Frame = 1 

Query: 3   LDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKIDR 62
           LDPAL  AE    +I V   G G FKT+T+AI SVPA N KRV+I +  G Y+EK+ IDR
Sbjct: 53  LDPALVAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAHGEYREKVTIDR 112

Query: 63  NKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVR 122
           NKPF+TL G  P  MP +T+DG AAKYGTV SA+LI+ +DYF A N++++N++P PDG  
Sbjct: 113 NKPFITLMGQ-PNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKT 172

Query: 123 KGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLN 182
           KGAQAL+ R  G  AA YNCKF GFQDT+CDD G HF+KDC+++GT DFIFG GTS+YL 
Sbjct: 173 KGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLG 232

Query: 183 TQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYT 242
           TQL V GD G+ VI AH+ +   + SGYSFVHC +TGTGG   YLGRAWM   +VV+AYT
Sbjct: 233 TQLHVVGD-GIRVIAAHAGKSAEEKSGYSFVHCKVTGTGG-GIYLGRAWMSHPKVVYAYT 292

Query: 243 TIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGLEY 302
            +  +++P GW + K    DKTV +GEYKCSGPG+    RV + + + D E   FL L Y
Sbjct: 293 EMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLSLGY 352

Query: 303 VQSEKWLLPPPS 315
           +Q  KWLLPPP+
Sbjct: 353 IQGSKWLLPPPA 360

[Close]
BLAST of Cucsa.086750 vs. TAIR10
Match: AT5G07430.1 (AT5G07430.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 330.9 bits (847), Expect = 8.8e-91
Identity = 167/311 (53.70%), Postives = 211/311 (67.85%), Query Frame = 1 

Query: 3   LDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKIDR 62
           LDPAL  AE    +I V   G  NFKT+ EAI S+P  NK RV+I +  GVY EK+ ID 
Sbjct: 53  LDPALEAAEAARQIITVNQKGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDI 112

Query: 63  NKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVR 122
            +PF+TL G  P     LT+ G AA+YGTV SATLIV A+YF AA+L I+N++P P    
Sbjct: 113 ARPFITLLGQ-PGAETVLTYHGTAAQYGTVESATLIVWAEYFQAAHLTIKNTAPMPKPGS 172

Query: 123 KGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLN 182
           +G QALA R    KAA Y+C+F GFQDTLCDD G HF+KDC+I+GT DFIFG+G SLYLN
Sbjct: 173 QG-QALAMRINADKAAFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLN 232

Query: 183 TQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYT 242
           TQL   GD GL VITA  R+   + +GY+FVHC +TGT G   YLGR+WM   +VV+A+T
Sbjct: 233 TQLHAVGD-GLRVITAQGRQSATEQNGYTFVHCKVTGT-GTGIYLGRSWMSHPKVVYAFT 292

Query: 243 TIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGLEY 302
            +  +++P GW +  + G+DKTV +GEYKC GPG+    RV Y + +   EV PFL L Y
Sbjct: 293 EMTSVVNPSGWRENLNRGYDKTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGY 352

Query: 303 VQSEKWLLPPP 314
           ++   WLLPPP
Sbjct: 353 IKGSTWLLPPP 359

[Close]
BLAST of Cucsa.086750 vs. TAIR10
Match: AT5G07420.1 (AT5G07420.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 324.3 bits (830), Expect = 8.3e-89
Identity = 164/312 (52.56%), Postives = 210/312 (67.31%), Query Frame = 1 

Query: 3   LDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKIDR 62
           LDP L  AE +  VI V  +G G+FKT+  AI S+P  NK RV+I +  G+Y EK+ +D 
Sbjct: 53  LDPELEAAEASRRVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDV 112

Query: 63  NKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVR 122
            +P+VTL G  P     LT+ G AAKYGTV SATLIV A  F AANL I N+SP P    
Sbjct: 113 GRPYVTLLGK-PGAETNLTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMPKPGT 172

Query: 123 KGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLN 182
           +G QALA R  G KAA YNC+F GFQDTLCDD G HF+K+C+I+GT DFIFG+G SLYL 
Sbjct: 173 QG-QALAMRINGDKAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLT 232

Query: 183 TQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYT 242
           TQL   GD GL VI AH+R+   + +GYSFVHC +TG  G   YLGRAWM   +VV++YT
Sbjct: 233 TQLHAVGD-GLRVIAAHNRQSTTEQNGYSFVHCKVTGV-GTGIYLGRAWMSHPKVVYSYT 292

Query: 243 TIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGLEY 302
            ++ +++P GW + +    DKTV +GEY C+GPG+    RVA+ + + + E   FL L Y
Sbjct: 293 EMSSVVNPSGWQENRVRAHDKTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGY 352

Query: 303 VQSEKWLLPPPS 315
           ++  KWLLPPP+
Sbjct: 353 IKGSKWLLPPPA 360

[Close]
BLAST of Cucsa.086750 vs. NCBI nr
Match: gi|778716816|ref|XP_011657599.1| (PREDICTED: putative pectinesterase 63 [Cucumis sativus])

HSP 1 Score: 649.4 bits (1674), Expect = 3.2e-183
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 1 

Query: 1   AELDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKI 60
           AELDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKI
Sbjct: 54  AELDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKI 113

Query: 61  DRNKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDG 120
           DRNKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDG
Sbjct: 114 DRNKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDG 173

Query: 121 VRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLY 180
           VRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLY
Sbjct: 174 VRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLY 233

Query: 181 LNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFA 240
           LNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFA
Sbjct: 234 LNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFA 293

Query: 241 YTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGL 300
           YTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGL
Sbjct: 294 YTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGL 353

Query: 301 EYVQSEKWLLPPPSPQV 318
           EYVQSEKWLLPPPSPQV
Sbjct: 354 EYVQSEKWLLPPPSPQV 370

[Close]
BLAST of Cucsa.086750 vs. NCBI nr
Match: gi|659097434|ref|XP_008449624.1| (PREDICTED: pectinesterase PPME1-like [Cucumis melo])

HSP 1 Score: 595.9 bits (1535), Expect = 4.2e-167
Identity = 289/317 (91.17%), Postives = 303/317 (95.58%), Query Frame = 1 

Query: 1   AELDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKI 60
           AELDPAL  AEENAT+IKVMSDGTG+FKT+TEAIASVPADNKKRV+I IG G+YKEKLKI
Sbjct: 53  AELDPALVAAEENATIIKVMSDGTGDFKTITEAIASVPADNKKRVMISIGDGIYKEKLKI 112

Query: 61  DRNKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDG 120
           DRNKPFVTLYGS PK MPKLTFDGDAAK+GTVYSATLIVEADYFTAANL+IENSSPRPDG
Sbjct: 113 DRNKPFVTLYGS-PKGMPKLTFDGDAAKFGTVYSATLIVEADYFTAANLVIENSSPRPDG 172

Query: 121 VRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLY 180
           VRKGAQALAARFMGTKAAIYNCKF GFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLY
Sbjct: 173 VRKGAQALAARFMGTKAAIYNCKFTGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLY 232

Query: 181 LNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFA 240
           +NTQLDVAGDGGLAVITAHSREQE+D SGYSFVHCSITGTGGKNT+LGRAWMPRSRVVFA
Sbjct: 233 VNTQLDVAGDGGLAVITAHSREQESDGSGYSFVHCSITGTGGKNTFLGRAWMPRSRVVFA 292

Query: 241 YTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGL 300
           YTT+ADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPG+VST RV YGKQL+D EV PFL L
Sbjct: 293 YTTMADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGSVSTERVPYGKQLSDAEVNPFLSL 352

Query: 301 EYVQSEKWLLPPPSPQV 318
            YVQSEKWLLPPP+PQV
Sbjct: 353 AYVQSEKWLLPPPNPQV 368

[Close]
BLAST of Cucsa.086750 vs. NCBI nr
Match: gi|659097432|ref|XP_008449623.1| (PREDICTED: putative pectinesterase 63 [Cucumis melo])

HSP 1 Score: 542.7 bits (1397), Expect = 4.2e-151
Identity = 260/317 (82.02%), Postives = 283/317 (89.27%), Query Frame = 1 

Query: 1   AELDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKI 60
           AELDP L  AEE+ATVIKV  DG+G+FKT+ EAI SVP  N KR+VIWIG GVYKEKLKI
Sbjct: 54  AELDPELVAAEESATVIKVRGDGSGDFKTIAEAIESVPIGNTKRIVIWIGGGVYKEKLKI 113

Query: 61  DRNKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDG 120
           DRNKPFVTLYGS P NMP LTFDGDA+KYGTVYSATLIVEADYFTAANL+IENSSPRPDG
Sbjct: 114 DRNKPFVTLYGS-PNNMPNLTFDGDASKYGTVYSATLIVEADYFTAANLVIENSSPRPDG 173

Query: 121 VRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLY 180
           VRKGAQALAAR  G KAAIYNCKF+GFQDTLCDDDGLH YKDCFIQGTVDFIFGKGTSLY
Sbjct: 174 VRKGAQALAARIRGNKAAIYNCKFIGFQDTLCDDDGLHLYKDCFIQGTVDFIFGKGTSLY 233

Query: 181 LNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFA 240
           LNTQLDV G+GGL VI AHSR+QE+D SGYSFVHCSITGTGG+NTYLGRAW PRSRVVFA
Sbjct: 234 LNTQLDVVGNGGLGVIAAHSRDQESDGSGYSFVHCSITGTGGRNTYLGRAWRPRSRVVFA 293

Query: 241 YTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGL 300
           YTT+ADIIHPEGWNDM H G+DKTV+FGEYKC GPG+ S+ RV Y KQL+  EV+PF+ L
Sbjct: 294 YTTMADIIHPEGWNDMNHFGYDKTVLFGEYKCWGPGSKSSERVKYSKQLSYEEVEPFVSL 353

Query: 301 EYVQSEKWLLPPPSPQV 318
            YVQSEKWLLPPP+P++
Sbjct: 354 GYVQSEKWLLPPPNPEI 369

[Close]
BLAST of Cucsa.086750 vs. NCBI nr
Match: gi|778716823|ref|XP_004140273.2| (PREDICTED: putative pectinesterase 63 [Cucumis sativus])

HSP 1 Score: 539.7 bits (1389), Expect = 3.5e-150
Identity = 261/316 (82.59%), Postives = 288/316 (91.14%), Query Frame = 1 

Query: 1   AELDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKI 60
           AELDPA+  AE N T++KVMSDGTG+FKTVTEAIASVP +NK RVVIWIG GVYKEKL I
Sbjct: 55  AELDPAVVAAEGNVTIVKVMSDGTGDFKTVTEAIASVPVNNKNRVVIWIGEGVYKEKLTI 114

Query: 61  DRNKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDG 120
           ++NKPF+TL G+ PKN+P LTFDG A+KYGTVYSATLIVEADYF AANLIIEN+SPRP+G
Sbjct: 115 EKNKPFITLCGT-PKNVPTLTFDGVASKYGTVYSATLIVEADYFVAANLIIENTSPRPNG 174

Query: 121 VRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLY 180
            RK AQALAARF GTK+A YNCKFLGFQDTLCDDDGLH YKDCFIQGTVDF+FGKGTSLY
Sbjct: 175 -RKEAQALAARFRGTKSAFYNCKFLGFQDTLCDDDGLHLYKDCFIQGTVDFVFGKGTSLY 234

Query: 181 LNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFA 240
           LNT+L+V G+G  AVITAHSREQEAD SGYSFVHCSITG G K+T+LGRAWMPRSRV+FA
Sbjct: 235 LNTELNVVGEGQFAVITAHSREQEADASGYSFVHCSITGNG-KDTFLGRAWMPRSRVIFA 294

Query: 241 YTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGL 300
           YT++ DIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLT+ EVKP+L L
Sbjct: 295 YTSMIDIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTEAEVKPYLSL 354

Query: 301 EYVQSEKWLLPPPSPQ 317
           E+VQS KWLLPPP+PQ
Sbjct: 355 EFVQSAKWLLPPPNPQ 367

[Close]
BLAST of Cucsa.086750 vs. NCBI nr
Match: gi|778716819|ref|XP_004140272.2| (PREDICTED: putative pectinesterase 63 [Cucumis sativus])

HSP 1 Score: 537.7 bits (1384), Expect = 1.3e-149
Identity = 259/316 (81.96%), Postives = 287/316 (90.82%), Query Frame = 1 

Query: 1   AELDPALATAEENATVIKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKI 60
           AELDPA+  AE N T++KVM DGTG+FKT+TEAIASVP +NK RVVIWIG GVYKEKL I
Sbjct: 55  AELDPAVVAAEGNVTIVKVMGDGTGDFKTITEAIASVPVNNKNRVVIWIGEGVYKEKLTI 114

Query: 61  DRNKPFVTLYGSDPKNMPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDG 120
           ++NKPF+TL G+ PKN+P L+FDG A+KYGTVYSATLIVEADYF AANLIIEN+SPRP+G
Sbjct: 115 EKNKPFITLCGT-PKNVPTLSFDGVASKYGTVYSATLIVEADYFVAANLIIENTSPRPNG 174

Query: 121 VRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLY 180
            RK AQALAARF GTK+A YNCKFLGFQDTLCDDDGLH YKDCFIQGTVDF+FGKGTSLY
Sbjct: 175 -RKEAQALAARFRGTKSAFYNCKFLGFQDTLCDDDGLHLYKDCFIQGTVDFVFGKGTSLY 234

Query: 181 LNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFA 240
           LNT+L+V G+G  AVITAHSREQEAD SGYSFVHCSITG G K+TYLGRAWMPRSRV+FA
Sbjct: 235 LNTELNVVGEGQFAVITAHSREQEADASGYSFVHCSITGNG-KDTYLGRAWMPRSRVIFA 294

Query: 241 YTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGL 300
           YT++ DIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLT+ EVKP+L L
Sbjct: 295 YTSMIDIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTEAEVKPYLSL 354

Query: 301 EYVQSEKWLLPPPSPQ 317
           E+VQS KWLLPPP+PQ
Sbjct: 355 EFVQSAKWLLPPPNPQ 367

The following BLAST results are available for this feature:
BLAST of Cucsa.086750 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PME63_ARATH2.1e-10260.47Putative pectinesterase 63 OS=Arabidopsis thaliana GN=PME63 PE=3 SV=2[more]
PPME1_ARATH1.2e-10057.05Pectinesterase PPME1 OS=Arabidopsis thaliana GN=PPME1 PE=1 SV=1[more]
PME48_ARATH4.4e-10056.73Probable pectinesterase 48 OS=Arabidopsis thaliana GN=PME48 PE=2 SV=2[more]
AL11B_OLEEU1.8e-9355.13Pectinesterase 2 OS=Olea europaea PE=1 SV=1[more]
AL11A_OLEEU4.0e-9354.49Pectinesterase 1 OS=Olea europaea PE=1 SV=1[more]
BLAST of Cucsa.086750 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KEH2_CUCSA2.2e-183100.00Pectinesterase OS=Cucumis sativus GN=Csa_6G428030 PE=4 SV=1[more]
A0A0A0KI78_CUCSA2.5e-15082.59Pectinesterase OS=Cucumis sativus GN=Csa_6G428290 PE=4 SV=1[more]
A0A0A0KJZ4_CUCSA9.4e-15081.96Pectinesterase OS=Cucumis sativus GN=Csa_6G428040 PE=4 SV=1[more]
A0A0A0KEY2_CUCSA3.6e-14179.23Pectinesterase OS=Cucumis sativus GN=Csa_6G428540 PE=4 SV=1[more]
M5XH72_PRUPE2.8e-11765.40Pectinesterase OS=Prunus persica GN=PRUPE_ppa007766mg PE=4 SV=1[more]
BLAST of Cucsa.086750 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G61680.11.2e-10360.47 Pectin lyase-like superfamily protein[more]
AT1G69940.16.5e-10257.05 Pectin lyase-like superfamily protein[more]
AT5G07410.12.5e-10156.73 Pectin lyase-like superfamily protein[more]
AT5G07430.18.8e-9153.70 Pectin lyase-like superfamily protein[more]
AT5G07420.18.3e-8952.56 Pectin lyase-like superfamily protein[more]
BLAST of Cucsa.086750 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778716816|ref|XP_011657599.1|3.2e-183100.00PREDICTED: putative pectinesterase 63 [Cucumis sativus][more]
gi|659097434|ref|XP_008449624.1|4.2e-16791.17PREDICTED: pectinesterase PPME1-like [Cucumis melo][more]
gi|659097432|ref|XP_008449623.1|4.2e-15182.02PREDICTED: putative pectinesterase 63 [Cucumis melo][more]
gi|778716823|ref|XP_004140273.2|3.5e-15082.59PREDICTED: putative pectinesterase 63 [Cucumis sativus][more]
gi|778716819|ref|XP_004140272.2|1.3e-14981.96PREDICTED: putative pectinesterase 63 [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000070Pectinesterase_cat
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005618 cell wall
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.086750.1Cucsa.086750.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 18..304
score: 8.3
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 14..310
score: 2.51
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 14..311
score: 1.8
NoneNo IPR availablePANTHERPTHR31321FAMILY NOT NAMEDcoord: 3..317
score: 4.2E
NoneNo IPR availablePANTHERPTHR31321:SF23PECTINESTERASE PPME1-RELATEDcoord: 3..317
score: 4.2E