Cucsa.079170 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.079170
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionLINE-1 reverse transcriptase isogeny
Locationscaffold00793 : 1800227 .. 1801215 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGTAAACCTTGTTCAGTATTCCATCATGATCAATGGCAAACCTAGAGGCAGAATTTTCCCAAACAGAGGAATCAGACAAGGAGATCCTTTATCCTCTTTCATTTTTGTGCTATCCATAGATTATCTTAGCAGGATTCTACAACATCTTGAGCAGCAGAAGCAAATAAAAGGTATCACAATAAAAGATTTAAATCTAACTCACCTTCTCTTTTCAGATGACATCTTGGTCTTTGTTGAAGATAGTGGTGAATATATTAGGAATCTTCAATTCGCTATTCACCTTTTTGAAAAGGCCATCGGCTTAAACATTAACCTCAACAAGTCCACCATCTCTCCAGTTAACGTTTCAAAAGAGAGATCAGATAAGATTGCAGTCAGTTAGGGGATAAAGACATATTTTCTCCCGATAACATACTTAGGGATGCCTCTTGGGGGCAATCCAAGATCAAAACCTTTTTGGGACAATATTACAGAGAAGATTCAGAAAAAACTGAACAAATGGAAATATTCCTTCATCGCAAAGGGAGGTAGAATTACTTTCATTAACACATCACTTGTCAGTCTACCTATATATCAACTCTCTATGTTTAAAGCACCTACTCATGCATATAAAACCATTGAGAAATATTGGAGGAATTTTCTCTGGAAAGGTAACTACGACAACATCTGCATAACAGGAACTCAAAAAAACAACCTCAACCTTGTTAAATAGTCTAAAGTTACAAACTCTCTCGACTCTAGAGGATTGGGCACTACACCTCTTTCTGCTACAAATTTTGCTTTGCTCACAAAATGGCTATGGAGATATTTAAATGAACCAAATGCTCTCTGGAAATCTCTAATTGATGCAAAATACTGTTGTAGAGATATGAATATGTTTCCTATAGCTGCAAAATACAGTAGCTCCAATGCTCCATGGAGATGCATTGATTGGTTCAACTCAAAAATTAAGTGGAAAGTTAATAATGGCAGCTCTTTATCCTTCTAG

mRNA sequence

ttgtaaaccttgttcagtattccatcatgatcaatggcaaacctagaggcagaattttcccaaacagaggaatcagacaaggagatcctttatcctctttcatttttgtgctatccatagattatcttagcaggattctacaacatcttgagcagcagaagcaaataaaaggtatcacaataaaagatttaaatctaactcaccttctcttttcagatgacatcttggtctttgttgaagatagtggtgaatatattaggaatcttcaattcgctattcacctttttgaaaaggccatcggcttaaacattaacctcaacaagtccaccatctctccagttaacgggataaagacatattttctcccgataacatacttagggatgcctcttgggggcaatccaagatcaaaacctttttgggacaatattacagagaagattcagaaaaaactgaacaaatggaaatattccttcatcgcaaagggaggtagaattactttcattaacacatcacttgtcagtctacctatatatcaactctctatgtttaaagcacctactcatgcatataaaaccattgagaaatattggaggaattttctctggaaaggattgggcactacacctctttctgctacaaattttgctttgctcacaaaatggctatggagatatttaaatgaaccaaatgctctctggaaatctctaattgatgcaaaatactgttgtagagatatgaatatgtttcctatagctgcaaaatacagtagctccaatgctccatggagatgcattgattggttcaactcaaaaattaagtggaaagttaataatggcagctctttatccttctag

Coding sequence (CDS)

TTGTAAACCTTGTTCAGTATTCCATCATGATCAATGGCAAACCTAGAGGCAGAATTTTCCCAAACAGAGGAATCAGACAAGGAGATCCTTTATCCTCTTTCATTTTTGTGCTATCCATAGATTATCTTAGCAGGATTCTACAACATCTTGAGCAGCAGAAGCAAATAAAAGGTATCACAATAAAAGATTTAAATCTAACTCACCTTCTCTTTTCAGATGACATCTTGGTCTTTGTTGAAGATAGTGGTGAATATATTAGGAATCTTCAATTCGCTATTCACCTTTTTGAAAAGGCCATCGGCTTAAACATTAACCTCAACAAGTCCACCATCTCTCCAGTTAACGGGATAAAGACATATTTTCTCCCGATAACATACTTAGGGATGCCTCTTGGGGGCAATCCAAGATCAAAACCTTTTTGGGACAATATTACAGAGAAGATTCAGAAAAAACTGAACAAATGGAAATATTCCTTCATCGCAAAGGGAGGTAGAATTACTTTCATTAACACATCACTTGTCAGTCTACCTATATATCAACTCTCTATGTTTAAAGCACCTACTCATGCATATAAAACCATTGAGAAATATTGGAGGAATTTTCTCTGGAAAGGATTGGGCACTACACCTCTTTCTGCTACAAATTTTGCTTTGCTCACAAAATGGCTATGGAGATATTTAAATGAACCAAATGCTCTCTGGAAATCTCTAATTGATGCAAAATACTGTTGTAGAGATATGAATATGTTTCCTATAGCTGCAAAATACAGTAGCTCCAATGCTCCATGGAGATGCATTGATTGGTTCAACTCAAAAATTAAGTGGAAAGTTAATAATGGCAGCTCTTTATCCTTCTAG

Protein sequence

VNLVQYSIMINGKPRGRIFPNRGIRQGDPLSSFIFVLSIDYLSRILQHLEQQKQIKGITIKDLNLTHLLFSDDILVFVEDSGEYIRNLQFAIHLFEKAIGLNINLNKSTISPVNGIKTYFLPITYLGMPLGGNPRSKPFWDNITEKIQKKLNKWKYSFIAKGGRITFINTSLVSLPIYQLSMFKAPTHAYKTIEKYWRNFLWKGLGTTPLSATNFALLTKWLWRYLNEPNALWKSLIDAKYCCRDMNMFPIAAKYSSSNAPWRCIDWFNSKIKWKVNNGSSLSF*
Homology
[Close]
BLAST of Cucsa.079170 vs. Swiss-Prot
Match: LORF2_HUMAN (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens PE=1 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 8.1e-13
Identity = 60/213 (28.17%), Postives = 105/213 (49.30%), Query Frame = 1 

Query: 7   SIMINGKPRGRIFPNRGIRQGDPLSSFIFVLSIDYLSRILQHLEQQKQIKGITIKDLNLT 66
           +I++NG+         G RQG PLS  +F + ++ L+R ++   Q+K+IKGI +    + 
Sbjct: 640 NIILNGQKLEAFPLKTGTRQGCPLSPLLFNIVLEVLARAIR---QEKEIKGIQLGKEEVK 699

Query: 67  HLLFSDDILVFVEDSGEYIRNLQFAIHLFEKAIGLNINLNKS----------TISPVNGI 126
             LF+DD++V++E+     +NL   I  F K  G  IN+ KS          T S + G 
Sbjct: 700 LSLFADDMIVYLENPIVSAQNLLKLISNFSKVSGYKINVQKSQAFLYNNNRQTESQIMGE 759

Query: 127 KTYFLP---ITYLGMPLGGNPRS--KPFWDNITEKIQKKLNKWKYSFIAKGGRITFINTS 186
             + +    I YLG+ L  + +   K  +  + ++I++  NKWK    +  GRI  +  +
Sbjct: 760 LPFTIASKRIKYLGIQLTRDVKDLFKENYKPLLKEIKEDTNKWKNIPCSWVGRINIVKMA 819

Query: 187 LVSLPIYQLSM--FKAPTHAYKTIEKYWRNFLW 203
           ++   IY+ +    K P   +  +EK    F+W
Sbjct: 820 ILPKVIYRFNAIPIKLPMTFFTELEKTTLKFIW 849

[Close]
BLAST of Cucsa.079170 vs. Swiss-Prot
Match: LORF2_MOUSE (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus GN=Pol PE=1 SV=2)

HSP 1 Score: 75.5 bits (184), Expect = 1.1e-12
Identity = 60/215 (27.91%), Postives = 100/215 (46.51%), Query Frame = 1 

Query: 7   SIMINGKPRGRIFPNRGIRQGDPLSSFIFVLSIDYLSRILQHLEQQKQIKGITIKDLNLT 66
           +I +NG+    I    G RQG PLS ++F + ++ L+R ++   QQK+IKGI I    + 
Sbjct: 647 NIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEVLARAIR---QQKEIKGIQIGKEEVK 706

Query: 67  HLLFSDDILVFVEDSGEYIRNLQFAIHLFEKAIGLNINLNKS-------------TISPV 126
             L +DD++V++ D     R L   I+ F + +G  IN NKS              I   
Sbjct: 707 ISLLADDMIVYISDPKNSTRELLNLINSFGEVVGYKINSNKSMAFLYTKNKQAEKEIRET 766

Query: 127 NGIKTYFLPITYLGMPLGGNPRSKPFWD----NITEKIQKKLNKWKYSFIAKGGRITFIN 186
                    I YLG+ L      K  +D    ++ ++I++ L +WK    +  GRI  + 
Sbjct: 767 TPFSIVTNNIKYLGVTL--TKEVKDLYDKNFKSLKKEIKEDLRRWKDLPCSWIGRINIVK 826

Query: 187 TSLVSLPIYQLSM--FKAPTHAYKTIEKYWRNFLW 203
            +++   IY+ +    K PT  +  +E     F+W
Sbjct: 827 MAILPKAIYRFNAIPIKIPTQFFNELEGAICKFVW 856

[Close]
BLAST of Cucsa.079170 vs. Swiss-Prot
Match: LIN1_NYCCO (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 2.4e-12
Identity = 78/308 (25.32%), Postives = 135/308 (43.83%), Query Frame = 1 

Query: 7   SIMINGKPRGRIFPNR-GIRQGDPLSSFIFVLSIDYLSRILQHLEQQKQIKGITIKDLNL 66
           +I++NG  + + FP R G RQG PLS  +F + ++ L+  ++   ++K IKGI I    +
Sbjct: 639 NIILNGV-KLKSFPLRSGTRQGCPLSPLLFNIVMEVLAIAIR---EEKAIKGIHIGSEEI 698

Query: 67  THLLFSDDILVFVEDSGEYIRNLQFAIHLFEKAIGLNINLNKSTI-----------SPVN 126
              LF+DD++V++E++ +    L   I  +    G  IN +KS             +  +
Sbjct: 699 KLSLFADDMIVYLENTRDSTTKLLEVIKEYSNVSGYKINTHKSVAFIYTNNNQAEKTVKD 758

Query: 127 GIKTYFLP--ITYLGMPLGGNPRS--KPFWDNITEKIQKKLNKWKYSFIAKGGRITFINT 186
            I    +P  + YLG+ L  + +   K  ++ + ++I + +NKWK    +  GRI  +  
Sbjct: 759 SIPFTVVPKKMKYLGVYLTKDVKDLYKENYETLRKEIAEDVNKWKNIPCSWLGRINIVKM 818

Query: 187 SLVSLPIYQLSM--FKAPTHAYKTIEKYWRNFLWK----GLGTTPLSATNFA-------- 246
           S++   IY  +    KAP   +K +EK   +F+W      +  T LS  N A        
Sbjct: 819 SILPKAIYNFNAIPIKAPLSYFKDLEKIILHFIWNQKKPQIAKTLLSNKNKAGGITLPDL 878

Query: 247 --------LLTKWLWRYLNEPNALWKSLIDAKYCCRDMNMFPIAAKYSSSNAPWRCIDWF 275
                   + T W W + N    +W  +        +  M P    Y   + P + I W 
Sbjct: 879 RLYYKSIVIKTAWYW-HKNREVDVWNRI-------ENQEMDPATYHYLIFDKPIKNIQWG 934

[Close]
BLAST of Cucsa.079170 vs. Swiss-Prot
Match: M1250_ARATH (Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana GN=AtMg01250 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 3.2e-09
Identity = 28/67 (41.79%), Postives = 48/67 (71.64%), Query Frame = 1 

Query: 9  MINGKPRGRIFPNRGIRQGDPLSSFIFVLSIDYLSRILQHLEQQKQIKGITIKDLN--LT 68
          +ING P+G + P+RG+RQGDPLS ++F+L  + LS + +  ++Q ++ GI + + +  + 
Sbjct: 13 IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 69 HLLFSDD 74
          HLLF+DD
Sbjct: 73 HLLFADD 79

[Close]
BLAST of Cucsa.079170 vs. Swiss-Prot
Match: RNHX1_ARATH (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.2e-08
Identity = 42/185 (22.70%), Postives = 75/185 (40.54%), Query Frame = 1 

Query: 128 MPLGGNPRSKPFWDNITEKIQKKLNKWKYSFIAKGGRITFINTSLVSLPIYQLSMFKAPT 187
           MP+     +K  +  I E++  +++ W+   ++  GR+T     L S+P++ +S    P 
Sbjct: 1   MPVLQKRINKDTFGEILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQ 60

Query: 188 HAYKTIEKYWRNFLWK-----------------------GLGTTPLSATNFALLTKWLWR 247
                +++  R FLW                        GLG     + N AL++K  WR
Sbjct: 61  SILNRLDQLSRTFLWGSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWR 120

Query: 248 YLNEPNALWKSLIDAKYCCRDMNMFPIAAKYSSSNAPWRCI-----DWFNSKIKWKVNNG 285
            L E N+LW  ++  KY   ++          S ++ WR I     D  +  + W   +G
Sbjct: 121 LLQEKNSLWTLVLQKKYHVGEIRDSRWLIPKGSWSSTWRSIAIGLRDVVSHGVGWIPGDG 180

[Close]
BLAST of Cucsa.079170 vs. TrEMBL
Match: M5XUF8_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa015473mg PE=4 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 2.0e-50
Identity = 114/297 (38.38%), Postives = 164/297 (55.22%), Query Frame = 1 

Query: 6    YSIMINGKPRGRIFPNRGIRQGDPLSSFIFVLSIDYLSRILQHLEQQKQIKGITIKD--L 65
            +S+MING+PRG+I  +RG+RQGDPLS F+F L +D LSRI++  +   Q  G++     +
Sbjct: 873  FSVMINGRPRGKIRASRGLRQGDPLSPFLFTLVMDVLSRIMEKAQDTDQFHGLSPGHGMV 932

Query: 66   NLTHLLFSDDILVFVEDSGEYIRNLQFAIHLFEKAIGLNINLNKSTISPVN--------- 125
             ++HL F+DD + F+ED  EY  NL   + LF    G+ IN +K ++  +N         
Sbjct: 933  EVSHLQFADDTIFFIEDKEEYWNNLLQILELFCFVSGMKINKSKCSLVGINLDDGLLNEL 992

Query: 126  ----GIKTYFLPITYLGMPLGGNPRSKPFWDNITEKIQKKLNKWKYSFIAKGGRITFINT 185
                G +    P++YLG+PLGGNPR+  FWD + EK++ +L KWK + ++KGGR+T I  
Sbjct: 993  AGAWGCEVGAWPMSYLGLPLGGNPRAIKFWDPVVEKVENRLQKWKRACLSKGGRLTMIQA 1052

Query: 186  SLVSLPIYQLSMFKAPTHAYKTIEKYWRNFLWKGL----------------------GTT 245
             L S+PIY +S+F+ P      IEK  R+FLW+GL                      G  
Sbjct: 1053 VLCSIPIYYMSVFRIPIGVANRIEKLMRDFLWEGLDRKRNHAVSWEVVGKAKFCGGLGVG 1112

Query: 246  PLSATNFALLTKWLWRYLNEPNALWKSLIDAKYCCRDMNMFPIAAKYSSSNAPWRCI 266
             L A + AL  KWLWR+ NEP+ALW  +I + Y                +N PWR I
Sbjct: 1113 SLRARSAALRAKWLWRFPNEPHALWHKVIRSIYGL-------------DTNGPWRDI 1156

[Close]
BLAST of Cucsa.079170 vs. TrEMBL
Match: M5W647_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa019748mg PE=4 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 8.4e-49
Identity = 111/297 (37.37%), Postives = 162/297 (54.55%), Query Frame = 1 

Query: 6   YSIMINGKPRGRIFPNRGIRQGDPLSSFIFVLSIDYLSRILQHLEQQKQIKGIT--IKDL 65
           +S+MING+PRG+   +RG+RQGDPLS F+F L +D LSRI++  +      G++  +  +
Sbjct: 579 FSVMINGRPRGKFRASRGLRQGDPLSPFLFTLVMDVLSRIMEKAQDADMFHGLSPGLGMV 638

Query: 66  NLTHLLFSDDILVFVEDSGEYIRNLQFAIHLFEKAIGLNINLNKSTISPVN--------- 125
            ++HL F+DD + F+ED  EY  NL   + LF    G+ IN +K ++  +N         
Sbjct: 639 EVSHLQFADDTIFFIEDKDEYWNNLLQILELFCFVSGMEINKSKCSLVGINLDDGLLNEL 698

Query: 126 ----GIKTYFLPITYLGMPLGGNPRSKPFWDNITEKIQKKLNKWKYSFIAKGGRITFINT 185
               G +    P++YLG+PLGGNPR+  FWD + EK++ +L KWK + ++K GR+T I  
Sbjct: 699 AGAWGCEVGAWPMSYLGLPLGGNPRAIKFWDPVVEKVENRLQKWKRACLSKEGRLTMIQA 758

Query: 186 SLVSLPIYQLSMFKAPTHAYKTIEKYWRNFLWK----------------------GLGTT 245
            L S+PIY +S+F+ P      IEK  R+FLW+                      GLG  
Sbjct: 759 VLCSIPIYYMSLFRIPIGVANRIEKLMRDFLWEGLDGKRNHAVSWEVVGKAKFYGGLGVG 818

Query: 246 PLSATNFALLTKWLWRYLNEPNALWKSLIDAKYCCRDMNMFPIAAKYSSSNAPWRCI 266
            L A + AL  KWLWR+ NEP+ALW  +I + Y           A   S  + WR I
Sbjct: 819 SLRARSAALRAKWLWRFPNEPHALWHKVIRSIYGMDTNGWDAKPATRGSCRSLWRDI 875

[Close]
BLAST of Cucsa.079170 vs. TrEMBL
Match: M5WKV4_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa015607mg PE=4 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.1e-48
Identity = 119/323 (36.84%), Postives = 168/323 (52.01%), Query Frame = 1 

Query: 4   VQYSIMINGKPRGRIFPNRGIRQGDPLSSFIFVLSIDYLSRILQHLEQQKQIKGITI--K 63
           V +SIMINGKPRG+   +RG+RQGDPLS F+F L  D LSRI++  +    + GI     
Sbjct: 360 VNFSIMINGKPRGKFRASRGLRQGDPLSPFLFTLVSDVLSRIIERAQDVNLVHGIVSGHD 419

Query: 64  DLNLTHLLFSDDILVFVEDSGEYIRNLQFAIHLFEKAIGLNINLNKSTISPVN------- 123
            + ++HL F+DD +  ++   EY  NL   + LF +  G+ IN  KS I  +N       
Sbjct: 420 QVEVSHLQFADDTIFLLDGKEEYWLNLLQLLKLFCEVSGMKINKAKSCILGINFSTDALN 479

Query: 124 ------GIKTYFLPITYLGMPLGGNPRSKPFWDNITEKIQKKLNKWKYSFIAKGGRITFI 183
                 G +    P+ YLG+PLGGNPR+  FW+ + +K++K+L KWK + ++KGGR+T I
Sbjct: 480 NMAGSWGCEVGCWPMVYLGLPLGGNPRALNFWNPVMDKVEKRLQKWKRACLSKGGRLTLI 539

Query: 184 NTSLVSLPIYQLSMFKAPTHAYKTIEKYWRNFLWK-----------------------GL 243
              L S+P Y +S+FK P      +E+  RNFLW+                       GL
Sbjct: 540 QAVLSSIPSYYMSLFKMPIGVAAKVEQLMRNFLWEGLEDGKNCHLVRWERVTKSKEEGGL 599

Query: 244 GTTPLSATNFALLTKWLWRYLNEPNALWKSLIDAKYCCRDMNMFPIAAKYSSSNAPWRCI 285
           G   L   N AL  KWLWR+  EPN+LW  +I +KY               S   PWR I
Sbjct: 600 GIGSLRERNEALRAKWLWRFPLEPNSLWHRIIKSKYGIDSNGWDTKQIDKVSCRNPWREI 659

[Close]
BLAST of Cucsa.079170 vs. TrEMBL
Match: M5XHS0_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa020085mg PE=4 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.1e-48
Identity = 119/323 (36.84%), Postives = 168/323 (52.01%), Query Frame = 1 

Query: 4   VQYSIMINGKPRGRIFPNRGIRQGDPLSSFIFVLSIDYLSRILQHLEQQKQIKGITI--K 63
           V +SIMINGKPRG+   +RG+RQGDPLS F+F L  D LSRI++  +    + GI     
Sbjct: 596 VNFSIMINGKPRGKFRASRGLRQGDPLSPFLFTLVSDVLSRIIERAQDVNLVHGIVSGHD 655

Query: 64  DLNLTHLLFSDDILVFVEDSGEYIRNLQFAIHLFEKAIGLNINLNKSTISPVN------- 123
            + ++HL F+DD +  ++   EY  NL   + LF +  G+ IN  KS I  +N       
Sbjct: 656 QVEVSHLQFADDTIFLLDGKEEYWLNLLQLLKLFCEVSGMKINKAKSCILGINFSTDALN 715

Query: 124 ------GIKTYFLPITYLGMPLGGNPRSKPFWDNITEKIQKKLNKWKYSFIAKGGRITFI 183
                 G +    P+ YLG+PLGGNPR+  FW+ + +K++K+L KWK + ++KGGR+T I
Sbjct: 716 NMAGSWGCEVGCWPMVYLGLPLGGNPRALNFWNPVMDKVEKRLQKWKRACLSKGGRLTLI 775

Query: 184 NTSLVSLPIYQLSMFKAPTHAYKTIEKYWRNFLWK-----------------------GL 243
              L S+P Y +S+FK P      +E+  RNFLW+                       GL
Sbjct: 776 QAVLSSIPSYYMSLFKMPIGVAAKVEQLMRNFLWEGLEDGKNCHLVRWERVTKSKEEGGL 835

Query: 244 GTTPLSATNFALLTKWLWRYLNEPNALWKSLIDAKYCCRDMNMFPIAAKYSSSNAPWRCI 285
           G   L   N AL  KWLWR+  EPN+LW  +I +KY               S   PWR I
Sbjct: 836 GIGSLRERNEALRAKWLWRFPLEPNSLWHRIIKSKYGIDSNGWDTKQIDKVSCRNPWREI 895

[Close]
BLAST of Cucsa.079170 vs. TrEMBL
Match: M5VS59_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa016504mg PE=4 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.9e-48
Identity = 119/323 (36.84%), Postives = 167/323 (51.70%), Query Frame = 1 

Query: 4   VQYSIMINGKPRGRIFPNRGIRQGDPLSSFIFVLSIDYLSRILQHLEQQKQIKGITI--K 63
           V +SIMINGKPRG+   +RG+RQGDPLS F+F L  D LSRI++  +    + GI     
Sbjct: 604 VNFSIMINGKPRGKFRASRGLRQGDPLSPFLFTLVSDVLSRIIERAQDVNLVHGIVSGHD 663

Query: 64  DLNLTHLLFSDDILVFVEDSGEYIRNLQFAIHLFEKAIGLNINLNKSTISPVN------- 123
            + ++HL F+DD +  ++   EY  NL   + LF    G+ IN  KS I  +N       
Sbjct: 664 QVEVSHLQFADDTIFLLDGKEEYWLNLLQLLKLFCDVSGMKINKAKSCILGINFSTEALN 723

Query: 124 ------GIKTYFLPITYLGMPLGGNPRSKPFWDNITEKIQKKLNKWKYSFIAKGGRITFI 183
                 G +    P+ YLG+PLGGNPR+  FW+ + +K++K+L KWK + ++KGGR+T I
Sbjct: 724 NMAGSWGCEVGCWPMVYLGLPLGGNPRALNFWNPVMDKVEKRLQKWKRACLSKGGRLTLI 783

Query: 184 NTSLVSLPIYQLSMFKAPTHAYKTIEKYWRNFLWK-----------------------GL 243
              L S+P Y +S+FK P      +E+  RNFLW+                       GL
Sbjct: 784 QAVLSSIPSYYMSLFKMPIGVAAKVEQLMRNFLWEGLEEGKNCHLVRWERVTKSKEEGGL 843

Query: 244 GTTPLSATNFALLTKWLWRYLNEPNALWKSLIDAKYCCRDMNMFPIAAKYSSSNAPWRCI 285
           G   L   N AL  KWLWR+  EPN+LW  +I +KY               S   PWR I
Sbjct: 844 GIGSLRERNEALRAKWLWRFPLEPNSLWHRIIKSKYGIDSNGWDTKQIDKVSCRNPWREI 903

[Close]
BLAST of Cucsa.079170 vs. TAIR10
Match: ATMG01250.1 (ATMG01250.1 RNA-directed DNA polymerase (reverse transcriptase))

HSP 1 Score: 63.9 bits (154), Expect = 1.8e-10
Identity = 28/67 (41.79%), Postives = 48/67 (71.64%), Query Frame = 1 

Query: 9  MINGKPRGRIFPNRGIRQGDPLSSFIFVLSIDYLSRILQHLEQQKQIKGITIKDLN--LT 68
          +ING P+G + P+RG+RQGDPLS ++F+L  + LS + +  ++Q ++ GI + + +  + 
Sbjct: 13 IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 69 HLLFSDD 74
          HLLF+DD
Sbjct: 73 HLLFADD 79

[Close]
BLAST of Cucsa.079170 vs. NCBI nr
Match: gi|659130833|ref|XP_008465376.1| (PREDICTED: putative ribonuclease H protein At1g65750 [Cucumis melo])

HSP 1 Score: 325.5 bits (833), Expect = 9.4e-86
Identity = 170/325 (52.31%), Postives = 207/325 (63.69%), Query Frame = 1 

Query: 1   VNLVQYSIMINGKPRGRIFPNRGIRQGDPLSSFIFVLSIDYLSRILQHLEQQKQIKGITI 60
           ++ VQY I++NG P+GRI   RGIRQGDPLS FIFV+++DYLSR+L HLE +  IKG++ 
Sbjct: 70  ISNVQYFILLNGSPKGRIKAERGIRQGDPLSPFIFVIAMDYLSRLLSHLETKGAIKGVSF 129

Query: 61  KDL-NLTHLLFSDDILVFVEDSGEYIRNLQFAIHLFEKAIGLNINLNKSTISPVN----- 120
            +  N++HLLF+DD+L+FVED+ +Y+ NLQ A+ LFEKA GL  N +KSTISP+N     
Sbjct: 130 NNCCNISHLLFADDVLIFVEDNEKYLSNLQMALSLFEKASGLTFNHSKSTISPINISADR 189

Query: 121 --------GIKTYFLPITYLGMPLGGNPRSKPFWDNITEKIQKKLNKWKYSFIAKGGRIT 180
                   G +T FLP  YLG+PLGGNPRS+ FW    E I KKLN WKYS I+K GR+T
Sbjct: 190 TDQIARFFGFQTKFLPFNYLGVPLGGNPRSRSFWGQTIECIHKKLNGWKYSQISKRGRLT 249

Query: 181 FINTSLVSLPIYQLSMFKAPTHAYKTIEKYWRNFLWKG---------------------- 240
            +  SL SLP YQLS FKAP   YK IEK+WR+FLW G                      
Sbjct: 250 LLKASLSSLPTYQLSTFKAPVSVYKEIEKHWRDFLWGGSEDKQNAHLVNWRICTSPKELG 309

Query: 241 -LGTTPLSATNFALLTKWLWRYLNEPNALWKSLIDAKYCCRDMNMFPIAAKYSSSNAPWR 285
            LG + L  TN ALL KWLWRY NE N+LWK  IDAKY        PI  +  S  +PW 
Sbjct: 310 GLGISKLKDTNQALLCKWLWRYHNESNSLWKKCIDAKYTKNHQGDIPIVGRNCSVKSPWN 369

[Close]
BLAST of Cucsa.079170 vs. NCBI nr
Match: gi|659093714|ref|XP_008447678.1| (PREDICTED: uncharacterized protein LOC103490087 [Cucumis melo])

HSP 1 Score: 275.8 bits (704), Expect = 8.6e-71
Identity = 144/287 (50.17%), Postives = 182/287 (63.41%), Query Frame = 1 

Query: 39  IDYLSRILQHLEQQKQIKGITI-KDLNLTHLLFSDDILVFVEDSGEYIRNLQFAIHLFEK 98
           +DYLSR+L HLE    IKG+ +  D N++H+LF+DDIL+FVED+  Y++NL+ AI LFEK
Sbjct: 1   MDYLSRLLSHLESTGAIKGVCLANDCNISHILFADDILLFVEDNDLYLKNLRMAISLFEK 60

Query: 99  AIGLNINLNKSTISPVN-------------GIKTYFLPITYLGMPLGGNPRSKPFWDNIT 158
           A GL INL+KS + PVN              I  + LP+TYLG+PLGGNP+SK FW NI 
Sbjct: 61  ASGLKINLSKSVMVPVNVPWPRALECASSWEISCHTLPLTYLGVPLGGNPKSKLFWRNIE 120

Query: 159 EKIQKKLNKWKYSFIAKGGRITFINTSLVSLPIYQLSMFKAPTHAYKTIEKYWRNFLWKG 218
           ++IQKKL+ WKY+ I+KGGR+T I ++L SLPIYQLS+F+AP+  YK IEK WRNFLWKG
Sbjct: 121 DRIQKKLSNWKYAHISKGGRLTLIKSTLSSLPIYQLSVFQAPSSTYKNIEKLWRNFLWKG 180

Query: 219 -----------------------LGTTPLSATNFALLTKWLWRYLNEPNALWKSLIDAKY 278
                                  LG + L  TN ALL+KWLWRY +EPN+LW+ LI  KY
Sbjct: 181 SCGSKGSHLINWAIVTKPKEEGELGISKLQITNQALLSKWLWRYYSEPNSLWRRLIHIKY 240

Query: 279 CCRDMNMFPIAAKYSSSNAPWRC----IDWFNSKIKWKVNNGSSLSF 285
             +     P     SS  APWR     IDWF     W +NNG  +SF
Sbjct: 241 QGKHPGDIPSNISSSSPKAPWRSIINNIDWFKRNQGWDLNNGDQISF 287

[Close]
BLAST of Cucsa.079170 vs. NCBI nr
Match: gi|659096604|ref|XP_008449185.1| (PREDICTED: putative ribonuclease H protein At1g65750 [Cucumis melo])

HSP 1 Score: 229.6 bits (584), Expect = 7.0e-57
Identity = 130/263 (49.43%), Postives = 162/263 (61.60%), Query Frame = 1 

Query: 21  NRGIRQGDP------LSSFIFVLSIDYLSRILQHLEQQKQIKGITI-KDLNLTHLLFSDD 80
           N+G     P      +S FIFVL++DYLS ++  LE + +I G+     LNLTH+LF+DD
Sbjct: 3   NQGAESNHPEAFDKVISPFIFVLAMDYLSHLITDLEGKGKIGGVKFGPKLNLTHILFADD 62

Query: 81  ILVFVEDSGEYIRNLQFAIHLFEKAIGLNINLNKSTISPVN-------------GIKTYF 140
           IL+FVED+ EYI NL+ A+ LFE A GLNINLNKSTI P+N             G++   
Sbjct: 63  ILIFVEDNEEYISNLKMALLLFESASGLNINLNKSTIFPINVPVNRANIIVESWGLRKDI 122

Query: 141 LPITYLGMPLGGNPRSKPFWDNITEKIQKKLNKWKYSFIAKGGRITFINTSLVSLPIYQL 200
           LP +YLGMPLGGN  S  FWDNI +KIQKKL+ WKYS ++KGGRIT IN++L SLP YQL
Sbjct: 123 LPTSYLGMPLGGNLSSLNFWDNILQKIQKKLSSWKYSQLSKGGRITLINSTLESLPTYQL 182

Query: 201 SMFKAPTHAYKTIEKYWRNFLWK-----------------------GLGTTPLSATNFAL 241
           S+FKA     + IE  WRNFLWK                       GLG   + +TN  L
Sbjct: 183 SVFKALKGIARKIEASWRNFLWKGTSNGHNISLIRWAKIITPKEKGGLGIHSVHSTNSTL 242

[Close]
BLAST of Cucsa.079170 vs. NCBI nr
Match: gi|596221970|ref|XP_007224079.1| (hypothetical protein PRUPE_ppa015473mg, partial [Prunus persica])

HSP 1 Score: 207.6 bits (527), Expect = 2.9e-50
Identity = 114/297 (38.38%), Postives = 164/297 (55.22%), Query Frame = 1 

Query: 6    YSIMINGKPRGRIFPNRGIRQGDPLSSFIFVLSIDYLSRILQHLEQQKQIKGITIKD--L 65
            +S+MING+PRG+I  +RG+RQGDPLS F+F L +D LSRI++  +   Q  G++     +
Sbjct: 873  FSVMINGRPRGKIRASRGLRQGDPLSPFLFTLVMDVLSRIMEKAQDTDQFHGLSPGHGMV 932

Query: 66   NLTHLLFSDDILVFVEDSGEYIRNLQFAIHLFEKAIGLNINLNKSTISPVN--------- 125
             ++HL F+DD + F+ED  EY  NL   + LF    G+ IN +K ++  +N         
Sbjct: 933  EVSHLQFADDTIFFIEDKEEYWNNLLQILELFCFVSGMKINKSKCSLVGINLDDGLLNEL 992

Query: 126  ----GIKTYFLPITYLGMPLGGNPRSKPFWDNITEKIQKKLNKWKYSFIAKGGRITFINT 185
                G +    P++YLG+PLGGNPR+  FWD + EK++ +L KWK + ++KGGR+T I  
Sbjct: 993  AGAWGCEVGAWPMSYLGLPLGGNPRAIKFWDPVVEKVENRLQKWKRACLSKGGRLTMIQA 1052

Query: 186  SLVSLPIYQLSMFKAPTHAYKTIEKYWRNFLWKGL----------------------GTT 245
             L S+PIY +S+F+ P      IEK  R+FLW+GL                      G  
Sbjct: 1053 VLCSIPIYYMSVFRIPIGVANRIEKLMRDFLWEGLDRKRNHAVSWEVVGKAKFCGGLGVG 1112

Query: 246  PLSATNFALLTKWLWRYLNEPNALWKSLIDAKYCCRDMNMFPIAAKYSSSNAPWRCI 266
             L A + AL  KWLWR+ NEP+ALW  +I + Y                +N PWR I
Sbjct: 1113 SLRARSAALRAKWLWRFPNEPHALWHKVIRSIYGL-------------DTNGPWRDI 1156

[Close]
BLAST of Cucsa.079170 vs. NCBI nr
Match: gi|658030603|ref|XP_008350747.1| (PREDICTED: uncharacterized protein LOC103414113 [Malus domestica])

HSP 1 Score: 205.7 bits (522), Expect = 1.1e-49
Identity = 118/322 (36.65%), Postives = 174/322 (54.04%), Query Frame = 1 

Query: 6    YSIMINGKPRGRIFPNRGIRQGDPLSSFIFVLSIDYLSRILQHLEQQKQIKGITI--KDL 65
            +S++ING+PRG+   +RG+RQGDPLS F+F L +D LSR+++  ++   IKG+ I  + +
Sbjct: 1168 FSVLINGRPRGKFQASRGLRQGDPLSPFLFTLVVDVLSRLMEKAQENHLIKGLCIGQEKV 1227

Query: 66   NLTHLLFSDDILVFV-EDSGEYIRNLQFAIHLFEKAIGLNINLNKSTISPVN-------- 125
             + HL F+DD + F+ ED G +  NL   + LF    GL IN  K  ++ +N        
Sbjct: 1228 EIXHLQFADDTIFFLAEDEGGW-NNLLELLKLFCSVSGLKINKAKCYLAGINSDCEKLNR 1287

Query: 126  -----GIKTYFLPITYLGMPLGGNPRSKPFWDNITEKIQKKLNKWKYSFIAKGGRITFIN 185
                 G +    PI YLG+PLGG PR+  FWD + +K++K+L  WK +F+++GGR+T I 
Sbjct: 1288 LADSWGCEVGSWPIKYLGLPLGGRPRALKFWDPVVDKMEKRLQSWKKAFLSRGGRLTLIQ 1347

Query: 186  TSLVSLPIYQLSMFKAPTHAYKTIEKYWRNFLWK-----------------------GLG 245
            + L SLP Y +S+FK P      +EK  + FLW+                       GLG
Sbjct: 1348 SVLGSLPTYYMSLFKMPCGVIGRLEKLMKGFLWEGVEEGKKNNLVKWEIVIKSKEEGGLG 1407

Query: 246  TTPLSATNFALLTKWLWRYLNEPNALWKSLIDAKYCCRDMNMFPIAAKYSSSNAPWRCI- 285
               L   N ALL KWLWR+  EP++LW  +I +KY  +D           SS +PW+ I 
Sbjct: 1408 VGNLRNRNEALLAKWLWRFPKEPHSLWHKVIRSKYGLQDNGWNAFPPIRGSSRSPWKDIS 1467

The following BLAST results are available for this feature:
BLAST of Cucsa.079170 vs. Swiss-Prot
Match NameE-valueIdentityDescription
LORF2_HUMAN8.1e-1328.17LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens PE=1 SV=1[more]
LORF2_MOUSE1.1e-1227.91LINE-1 retrotransposable element ORF2 protein OS=Mus musculus GN=Pol PE=1 SV=2[more]
LIN1_NYCCO2.4e-1225.32LINE-1 reverse transcriptase homolog OS=Nycticebus coucang PE=3 SV=1[more]
M1250_ARATH3.2e-0941.79Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana GN=AtMg0... [more]
RNHX1_ARATH1.2e-0822.70Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana GN=At1g65750 P... [more]
BLAST of Cucsa.079170 vs. TrEMBL
Match NameE-valueIdentityDescription
M5XUF8_PRUPE2.0e-5038.38Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa015473mg PE=4 S... [more]
M5W647_PRUPE8.4e-4937.37Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa019748mg PE=4 S... [more]
M5WKV4_PRUPE1.1e-4836.84Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa015607mg PE=4 S... [more]
M5XHS0_PRUPE1.1e-4836.84Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa020085mg PE=4 S... [more]
M5VS59_PRUPE1.9e-4836.84Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa016504mg PE=4 S... [more]
BLAST of Cucsa.079170 vs. TAIR10
Match NameE-valueIdentityDescription
ATMG01250.11.8e-1041.79ATMG01250.1 RNA-directed DNA polymerase (reverse transcriptase)[more]
BLAST of Cucsa.079170 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659130833|ref|XP_008465376.1|9.4e-8652.31PREDICTED: putative ribonuclease H protein At1g65750 [Cucumis melo][more]
gi|659093714|ref|XP_008447678.1|8.6e-7150.17PREDICTED: uncharacterized protein LOC103490087 [Cucumis melo][more]
gi|659096604|ref|XP_008449185.1|7.0e-5749.43PREDICTED: putative ribonuclease H protein At1g65750 [Cucumis melo][more]
gi|596221970|ref|XP_007224079.1|2.9e-5038.38hypothetical protein PRUPE_ppa015473mg, partial [Prunus persica][more]
gi|658030603|ref|XP_008350747.1|1.1e-4936.65PREDICTED: uncharacterized protein LOC103414113 [Malus domestica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000477RT_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044237 cellular metabolic process
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:1901363 heterocyclic compound binding
molecular_function GO:0097159 organic cyclic compound binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.079170.1Cucsa.079170.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 7..129
score: 3.0
IPR000477Reverse transcriptase domainPROFILEPS50878RT_POLcoord: 1..130
score: 11
NoneNo IPR availablePANTHERPTHR19446REVERSE TRANSCRIPTASEScoord: 1..163
score: 1.1
NoneNo IPR availablePANTHERPTHR19446:SF366REVERSE TRANSCRIPTASE/RNA-DEPENDENT DNA POLYMERASE-RELATEDcoord: 1..163
score: 1.1
NoneNo IPR availableunknownSSF56672DNA/RNA polymerasescoord: 9..159
score: 4.3

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.079170CSPI07G13120Wild cucumber (PI 183967)cgycpiB079
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.079170Melon (DHL92) v3.6.1cgymedB080
Cucsa.079170Melon (DHL92) v3.6.1cgymedB084
Cucsa.079170Silver-seed gourdcarcgyB0341
Cucsa.079170Silver-seed gourdcarcgyB1128
Cucsa.079170Cucumber (Chinese Long) v3cgycucB080
Cucsa.079170Watermelon (97103) v2cgywmbB078
Cucsa.079170Wax gourdcgywgoB092
Cucsa.079170Wax gourdcgywgoB094
Cucsa.079170Cucurbita maxima (Rimu)cgycmaB0133
Cucsa.079170Cucurbita maxima (Rimu)cgycmaB0139
Cucsa.079170Cucurbita moschata (Rifu)cgycmoB0130
Cucsa.079170Cucurbita moschata (Rifu)cgycmoB0135
Cucsa.079170Cucumber (Chinese Long) v2cgycuB076
Cucsa.079170Melon (DHL92) v3.5.1cgymeB081
Cucsa.079170Melon (DHL92) v3.5.1cgymeB086
Cucsa.079170Watermelon (Charleston Gray)cgywcgB081
Cucsa.079170Watermelon (97103) v1cgywmB083
Cucsa.079170Cucurbita pepo (Zucchini)cgycpeB0137
Cucsa.079170Cucurbita pepo (Zucchini)cgycpeB0139
Cucsa.079170Bottle gourd (USVL1VR-Ls)cgylsiB081