Cucsa.065410 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.065410 vs. Swiss-Prot
Match: CWC22_DANRE (Pre-mRNA-splicing factor CWC22 homolog OS=Danio rerio GN=cwc22 PE=2 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 2.4e-203
Identity = 372/803 (46.33%), Postives = 524/803 (65.26%), Query Frame = 1

Query: 2   DRKHSRESSDEDEVKGNRRTSRDNDRQDRDKRH--------------------QDKNLHR 61
           D   S E  ++ + K +  +S D + +D D+R                           R
Sbjct: 20  DEPPSTEEIEQRQRKASTSSSEDGEHEDNDRRRVVGSPGSRDGSPRVGSPVARASPRAKR 79

Query: 62  EVERGDKKKSYESFDEGEQRTERTKSRHDRDRRHHDEKRHSRGQERENRKESYQSSDEDE 121
           + +  D        D+G  R  R+   H R  R  DE+  +R  ER +  + ++   +  
Sbjct: 80  DQKSSDSDSDSSDSDDGVMRKIRSSVMHIR--RTSDEETKNR--ERSSSPDRHEKKSKSR 139

Query: 122 RKNRRHDRRQRETENENKNYDRRLDHDSKDERDKELLRKSDHRRNRTDSDRERRHSKHDY 181
            ++R   R +  + +  + Y R     ++  R++E  R  D  R    S RER     D+
Sbjct: 140 SRSRSRSRSRSRSRSPRERYRR-----ARRSRERERDRYGDRERYERRSSRER-----DW 199

Query: 182 KHNNFQDQRDKEASGRREDRRNIGEKEKVGTGTIDHLQPHRQEKQTENNLNPDSSNLGKS 241
           +H     +R + AS  + D+    E             P ++ K+T + +      L ++
Sbjct: 200 EHR----RRGRSASPDKNDKPPTEEP------------PVKKRKETLDPI------LTRT 259

Query: 242 GGVYIPPFKLARMMKEVQDKSSIEYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAEN 301
           GG YIPP KL  M  ++ DKSS+EYQR++W+AL+KSINGL+NKVN +NI NII EL  EN
Sbjct: 260 GGAYIPPAKLRMMQAQITDKSSLEYQRMSWEALKKSINGLINKVNVSNIANIIQELLQEN 319

Query: 302 LIRGRGLFCRSCMKSQMASPGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRND 361
           ++RGRGL  RS +++Q ASP FT V++A+VA++N+KFP++G+L+L+R++L  ++ Y+RND
Sbjct: 320 IVRGRGLLARSILQAQAASPIFTHVYSAVVAIINSKFPQIGELILKRLILNFRKGYRRND 379

Query: 362 KPQLLAAVKFIAHLVNQQVAHEIIALELLTVLLENPTDDSVEVAVGFVTECGSILKDLSP 421
           K Q L A KF+ HL+NQ VAHE++ LE+LT+LLE PTDDSVEVA+ F+ ECG  L ++SP
Sbjct: 380 KQQCLTASKFVGHLINQNVAHEVLCLEMLTLLLERPTDDSVEVAISFLKECGLKLTEVSP 439

Query: 422 KGLHGIFERFRGILHEGEIDKRVQFLIEGLFAIRKAKFEGYPAVRPELDLVEQEDQLTHE 481
           +G++ IFER R ILHE EIDKRVQ++IE +FAIRK  F+ +P +   LDLVE+EDQ TH 
Sbjct: 440 RGINAIFERLRNILHESEIDKRVQYMIEVMFAIRKDGFKDHPIIPEGLDLVEEEDQFTHM 499

Query: 482 ISLQEEIDPEITLDIFKSDPNFLENEKRYEDLKKNILGEESEDE-EDRSDAGSDESEDED 541
           + L++E + E  L++FK DPNFLENE++Y+ +K+ IL E S D  +D   +G DE +DE+
Sbjct: 500 LPLEDEYNTEDILNVFKLDPNFLENEEKYKTIKREILDEGSSDSGDDAGGSGDDEDDDEE 559

Query: 542 EEDESEEEDEEQMQINDETETNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEIEL 601
           +E+ +  E++E++ I D+TE NLV  RRTIYL I SS+DFEE  HKL+K+     Q  EL
Sbjct: 560 DEEAAAGEEQEKVTIFDQTEVNLVAFRRTIYLAIQSSLDFEECAHKLIKMDFPESQTKEL 619

Query: 602 CVMLLECCSQERTYLRYYGLLGQRFCKINKVYQENFDKCFVQQYSMIHRLETNKLRNVAK 661
           C M+L+CC+Q+RTY +++GLL  RFC + K Y E+F+  F +QY  IHRLETNKLRNVA+
Sbjct: 620 CNMILDCCAQQRTYEKFFGLLAGRFCLLKKEYMESFEAIFQEQYETIHRLETNKLRNVAR 679

Query: 662 FFAHLLGTDALPWHVLSYIRLTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLTDPTMQ 721
            FAHLL TD++PW VL  +R++E+ TTSSSRIF+KILFQEL  ++G+  LNERL D T+Q
Sbjct: 680 IFAHLLYTDSVPWSVLECVRMSEDTTTSSSRIFVKILFQELCAYMGLPKLNERLKDTTLQ 739

Query: 722 DSFESIFPRDNPKNTRFSINFFTSIGLGGLTENLREYLKNMPRLIMQQQKPVSDSDEESQ 781
             FE +FPRDNP+NTRF+INFFTSIGLGGLT+ LRE+LKN P++IM Q + V  SD  S 
Sbjct: 740 PFFEGLFPRDNPRNTRFAINFFTSIGLGGLTDELREHLKNAPKMIMTQNQEVESSDSSSS 786

Query: 782 SSDSSDSDSSS-SESKSDSEDSE 783
           SS SSDS SSS S S+SDS +S+
Sbjct: 800 SSSSSDSSSSSGSSSESDSSESD 786

HSP 2 Score: 55.1 bits (131), Expect = 4.2e-06
Identity = 42/193 (21.76%), Postives = 77/193 (39.90%), Query Frame = 1

Query: 2   DRKHSRESSDEDEVKGNRRTSRDNDRQDRDKRHQDKNLHREVERGDKKKSYESFDEGEQR 61
           D   S  SS + + +  + + +  D+    K  +  N    +E    K+      +  + 
Sbjct: 794 DSSSSSGSSSDSDSRRKKASGKKKDKSKSKKSSKAANQRSPLEERPTKRHENRRQDASKE 853

Query: 62  TERTKSRHDRDRRHHDEKRHSRGQERENRKESYQSSDEDERKNRRHDRRQRET---ENEN 121
             R   +H+RD +   ++  S         E  +   E  R  + H  R  ++   +++ 
Sbjct: 854 DRRGSDKHNRDPQRRGQQDESPPARPRGEPERIRGQKEPHRHAQDHQDRPTDSGRHKDDG 913

Query: 122 KNYDRRLDHDSKDERDKELLRKSDHRRNRTDSDRERRHSKHDYKHNNFQDQRDKE----- 181
           KN     D D +  R+KE LR+S  R    +  R+   S+  Y   N  ++ DKE     
Sbjct: 914 KNSRVNKDKDRRRSREKEPLRRSRDRSKSRERSRKEMDSRDSY--GNGLERADKENRHSD 973

Query: 182 ---ASGRREDRRN 184
               S R++DRR+
Sbjct: 974 RYKESRRKDDRRH 984

HSP 3 Score: 34.3 bits (77), Expect = 7.6e+00
Identity = 18/42 (42.86%), Postives = 26/42 (61.90%), Query Frame = 1

Query: 753 SDSDEESQSSDSSDSDSSSSESKSDSEDSEIEDRRREKKRRK 795
           S+SD +S SSDS  S SS S S SDS   +   ++++K + K
Sbjct: 783 SESDSDS-SSDSDSSSSSGSSSDSDSRRKKASGKKKDKSKSK 823

BLAST of Cucsa.065410 vs. Swiss-Prot
Match: CWC22_XENLA (Pre-mRNA-splicing factor CWC22 homolog OS=Xenopus laevis GN=cwc22 PE=2 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 5.5e-200
Identity = 367/764 (48.04%), Postives = 514/764 (67.28%), Query Frame = 1

Query: 45  RGDKKKSYESFDEGEQRTERTKSR--HDRDRRHHDEKRHSRGQERENRKESYQSSDEDER 104
           R   ++S    D  E+R+   + R   +R R + D  R+    E  +R   Y  SDE  R
Sbjct: 19  RSSSERSSSPEDSNEERSPSPRDRGYSNRSRDYSDRDRY----EDRSRSGRYDRSDESRR 78

Query: 105 KNRRHDRRQRETENENKNY-DRRLDHDSKDERDKELLRKSDHRRNRTDSDRERRHSKHDY 164
           + R     +R T   ++ Y DRR  +  +D    +  R  + R +R++ +RER       
Sbjct: 79  RER-----ERSTSPRDRGYTDRRRGYSDRDGYGND--RSRNGRYDRSEDNRERE------ 138

Query: 165 KHNNFQDQRDKEASGRREDRRNIGEKEKVGTGTIDHLQPHRQEKQTENNLNPDSSNLGKS 224
           K  N+QD+  ++ S     R     + +  T   +  +P  ++K+ E  L+P    L ++
Sbjct: 139 KRQNYQDRDYEKRSPPARRRSPPARRSEEQTEEQNQTEPPVKKKKEE--LDPI---LTRT 198

Query: 225 GGVYIPPFKLARMMKEVQDKSSIEYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAEN 284
           GG YIPP +L  M +++ DKSS+ YQR++W+AL+KSINGLVNKVN +NI NII EL  EN
Sbjct: 199 GGAYIPPARLRMMQEQITDKSSMAYQRMSWEALKKSINGLVNKVNVSNIGNIIQELLQEN 258

Query: 285 LIRGRGLFCRSCMKSQMASPGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRND 344
           ++RGRGL  RS +++Q ASP FT V+AALV+++N+KFP +G+L+L+R++L  ++ Y+RND
Sbjct: 259 IVRGRGLLARSVLQAQSASPIFTHVYAALVSIINSKFPHIGELILKRLILNFRKGYRRND 318

Query: 345 KPQLLAAVKFIAHLVNQQVAHEIIALELLTVLLENPTDDSVEVAVGFVTECGSILKDLSP 404
           K   L + KF+AHL+NQ VAHE++ALE+LT+LLE P DDSVEVA+GF+ E G  L  ++P
Sbjct: 319 KQLCLTSSKFVAHLINQNVAHEVLALEMLTLLLERPNDDSVEVAIGFLKESGLKLTQVTP 378

Query: 405 KGLHGIFERFRGILHEGEIDKRVQFLIEGLFAIRKAKFEGYPAVRPELDLVEQEDQLTHE 464
           +G++ IFER R ILHE EIDKRVQ++IE +FA+RK  F+ +P +   LDLVE+EDQ TH 
Sbjct: 379 RGINAIFERLRNILHESEIDKRVQYMIEVMFAVRKDGFKDHPVIPEGLDLVEEEDQFTHM 438

Query: 465 ISLQEEIDPEITLDIFKSDPNFLENEKRYEDLKKNILGE-ESEDEEDRSDAGSDESEDED 524
           + L+++ + E  L++FK DP+FLENE++Y+ +KK IL E +S+ E D ++   DESE+E+
Sbjct: 439 LPLEDDYNQEDVLNVFKMDPDFLENEEKYKAIKKEILDEGDSDSEGDANEGSEDESEEEE 498

Query: 525 EEDESEEEDEEQMQINDETETNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEIEL 584
           E+ +    + E+M I+D+TE NLV  RRTIYL I SS+DFEE  HKL+K+     Q  EL
Sbjct: 499 EDGQEAGTEGEKMTIHDKTEVNLVAFRRTIYLAIQSSLDFEECAHKLIKMDFPESQTKEL 558

Query: 585 CVMLLECCSQERTYLRYYGLLGQRFCKINKVYQENFDKCFVQQYSMIHRLETNKLRNVAK 644
           C M+L+CC+Q+RTY +++GLL  RFC + K Y E F+  F +Q+  IHRLETNKLRNVAK
Sbjct: 559 CNMILDCCAQQRTYEKFFGLLAGRFCLLKKEYLEAFENIFKEQFETIHRLETNKLRNVAK 618

Query: 645 FFAHLLGTDALPWHVLSYIRLTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLTDPTMQ 704
            FAHLL TD+LPW VL  + L+EE TTSSSRIF+KI FQEL E++G+  LN RL D T+Q
Sbjct: 619 MFAHLLYTDSLPWSVLECMNLSEETTTSSSRIFVKIFFQELCEYMGLPKLNARLKDVTLQ 678

Query: 705 DSFESIFPRDNPKNTRFSINFFTSIGLGGLTENLREYLKNMPRLIMQQQKPVSDSDEES- 764
             F+ + P DNPKNTRF+INFFTSIGLGGLT+ LRE+LKN P++IM Q++ V  SD  S 
Sbjct: 679 PFFQGLLPMDNPKNTRFAINFFTSIGLGGLTDELREHLKNAPKMIMTQKQNVESSDSSSD 738

Query: 765 ------QSSDSSDSDSSSSESKSDSEDSEIEDRRREKKRRKTRR 798
                  SSDS  S SSSSES S S DS+   R++  +++K  R
Sbjct: 739 SSSGSESSSDSDSSSSSSSESSSSSSDSDSSRRKKHSQKKKKSR 760

BLAST of Cucsa.065410 vs. Swiss-Prot
Match: CWC22_HUMAN (Pre-mRNA-splicing factor CWC22 homolog OS=Homo sapiens GN=CWC22 PE=1 SV=3)

HSP 1 Score: 695.7 bits (1794), Expect = 6.1e-199
Identity = 353/711 (49.65%), Postives = 485/711 (68.21%), Query Frame = 1

Query: 82  SRGQERENRKESYQSSDEDERKNRRHDRRQRETENENKNYDRRLDHDSKDERDKELLRKS 141
           S G +R     SYQ +   E +    +R  R+ +          D+   D       R  
Sbjct: 11  SSGHDRRENLNSYQRNSSPEDRYEEQERSPRDRDY--------FDYSRSDYEHSRRGRSY 70

Query: 142 DHRRNRTDSDRERRHSKHDYKHNNFQDQRDKEASGRREDRRNIGEKEKVGTGTIDHLQPH 201
           D      + DRE+R  +   +  + +  R   + GRR    ++ +            Q  
Sbjct: 71  DSSMESRNRDREKRRERE--RDTDRKRSRKSPSPGRRNPETSVTQSSSA--------QDE 130

Query: 202 RQEKQTENNLNPDSSNLGKSGGVYIPPFKLARMMKEVQDKSSIEYQRLTWDALRKSINGL 261
              K+ ++ L+P    L ++GG YIPP KL  M +++ DK+S+ YQR++W+AL+KSINGL
Sbjct: 131 PATKKKKDELDP---LLTRTGGAYIPPAKLRMMQEQITDKNSLAYQRMSWEALKKSINGL 190

Query: 262 VNKVNATNIKNIIPELFAENLIRGRGLFCRSCMKSQMASPGFTDVFAALVAVVNTKFPEV 321
           +NKVN +NI  II EL  EN++RGRGL  RS +++Q ASP FT V+AALVA++N+KFP++
Sbjct: 191 INKVNISNISIIIQELLQENIVRGRGLLSRSVLQAQSASPIFTHVYAALVAIINSKFPQI 250

Query: 322 GDLLLRRIVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQVAHEIIALELLTVLLENPTDDS 381
           G+L+L+R++L  ++ Y+RNDK   L A KF+AHL+NQ VAHE++ LE+LT+LLE PTDDS
Sbjct: 251 GELILKRLILNFRKGYRRNDKQLCLTASKFVAHLINQNVAHEVLCLEMLTLLLERPTDDS 310

Query: 382 VEVAVGFVTECGSILKDLSPKGLHGIFERFRGILHEGEIDKRVQFLIEGLFAIRKAKFEG 441
           VEVA+GF+ ECG  L  +SP+G++ IFER R ILHE EIDKRVQ++IE +FA+RK  F+ 
Sbjct: 311 VEVAIGFLKECGLKLTQVSPRGINAIFERLRNILHESEIDKRVQYMIEVMFAVRKDGFKD 370

Query: 442 YPAVRPELDLVEQEDQLTHEISLQEEIDPEITLDIFKSDPNFLENEKRYEDLKKNILGEE 501
           +P +   LDLVE++DQ TH + L+++ +PE  L++FK DPNF+ENE++Y+ +KK IL E 
Sbjct: 371 HPIILEGLDLVEEDDQFTHMLPLEDDYNPEDVLNVFKMDPNFMENEEKYKAIKKEILDEG 430

Query: 502 SEDEEDRSDAGSDESEDEDEEDESEEEDE-EQMQINDETETNLVNLRRTIYLTIMSSVDF 561
             D     DAGS E ++E+EE+E EE++E +++ I+D+TE NLV+ RRTIYL I SS+DF
Sbjct: 431 DTDSNTDQDAGSSEEDEEEEEEEGEEDEEGQKVTIHDKTEINLVSFRRTIYLAIQSSLDF 490

Query: 562 EEAGHKLLKIKLEPGQEIELCVMLLECCSQERTYLRYYGLLGQRFCKINKVYQENFDKCF 621
           EE  HKLLK++    Q  ELC M+L+CC+Q+RTY +++GLL  RFC + K Y E+F+  F
Sbjct: 491 EECAHKLLKMEFPESQTKELCNMILDCCAQQRTYEKFFGLLAGRFCMLKKEYMESFEGIF 550

Query: 622 VQQYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVLSYIRLTEEDTTSSSRIFIKILFQE 681
            +QY  IHRLETNKLRNVAK FAHLL TD+LPW VL  I+L+EE TTSSSRIF+KI FQE
Sbjct: 551 KEQYDTIHRLETNKLRNVAKMFAHLLYTDSLPWSVLECIKLSEETTTSSSRIFVKIFFQE 610

Query: 682 LSEHLGIRLLNERLTDPTMQDSFESIFPRDNPKNTRFSINFFTSIGLGGLTENLREYLKN 741
           L E++G+  LN RL D T+Q  FE + PRDNP+NTRF+INFFTSIGLGGLT+ LRE+LKN
Sbjct: 611 LCEYMGLPKLNARLKDETLQPFFEGLLPRDNPRNTRFAINFFTSIGLGGLTDELREHLKN 670

Query: 742 MPRLIMQQQKPV--SDSDEESQSSDSSDSDSSSSESKSDSEDSEIEDRRRE 790
            P++I+ Q+  V  + S   S SS SS S+S SS+S SDS DS  E    E
Sbjct: 671 TPKVIVAQKPDVEQNKSSPSSSSSASSSSESDSSDSDSDSSDSSSESSSEE 700

HSP 2 Score: 46.2 bits (108), Expect = 1.9e-03
Identity = 36/114 (31.58%), Postives = 52/114 (45.61%), Query Frame = 1

Query: 21  TSRDNDRQDRDKRHQDKNLHREVERGDKKKSY----ESFDEGEQRTERTKSRHDRDRRHH 80
           + R+N  +  + R Q+ ++  E + GD KK       SF E E+ T R K     D  + 
Sbjct: 792 SERNNYSRVANDRDQEMHIDLENKHGDPKKKRGERRNSFSENEKHTHRIK-----DSENF 851

Query: 81  DEKRHSRGQERENRKESYQSSDED------ERKNRRHDRRQRETENENKNYDRR 125
             K  S+ +E  NRK S   SDED      ER+  +  R   ++    KN DRR
Sbjct: 852 RRKDRSKSKEM-NRKHSGSRSDEDRYQNGAERRWEKSSRYSEQSRESKKNQDRR 899

HSP 3 Score: 39.7 bits (91), Expect = 1.8e-01
Identity = 33/127 (25.98%), Postives = 54/127 (42.52%), Query Frame = 1

Query: 20  RTSRDNDRQDRDKRHQ----DKNLHREVERGDKKKSYESFDEGEQRTERTKSRHDRDRRH 79
           + S  +DR++    +Q     ++ + E ER  + + Y  +        R+   H R  R 
Sbjct: 9   KPSSGHDRRENLNSYQRNSSPEDRYEEQERSPRDRDYFDYS-------RSDYEHSRRGRS 68

Query: 80  HDEKRHSRGQERENRKESYQSSD-EDERKNRRHDRRQRETE--NENKNYDRRLDHDSKDE 139
           +D    SR ++RE R+E  + +D +  RK+    RR  ET     +   D       KDE
Sbjct: 69  YDSSMESRNRDREKRRERERDTDRKRSRKSPSPGRRNPETSVTQSSSAQDEPATKKKKDE 128

HSP 4 Score: 36.6 bits (83), Expect = 1.5e+00
Identity = 27/123 (21.95%), Postives = 47/123 (38.21%), Query Frame = 1

Query: 49  KKSYESFDEGEQRTERTKSRHDRDRRHHDEKRHSRGQERENRKESYQSSDEDERKNRRHD 108
           K S  S       +E   S  D D      +  S   +  +      +S  D RK     
Sbjct: 665 KSSPSSSSSASSSSESDSSDSDSDSSDSSSESSSEESDSSSISSHSSASANDVRKKGHGK 724

Query: 109 RRQRETENENKNYDRRLDHDSKDERDKELLRKSDHRRNRTDSDRERRHSKHDYKHNNFQD 168
            R +E +        +L  + +    K+  R+ +H    T ++RERR  KH  ++++  +
Sbjct: 725 TRSKEVD--------KLIRNQQTNDRKQKERRQEHGHQETRTERERRSEKHRDQNSSGSN 779

Query: 169 QRD 172
            RD
Sbjct: 785 WRD 779

BLAST of Cucsa.065410 vs. Swiss-Prot
Match: CWC22_PONAB (Pre-mRNA-splicing factor CWC22 homolog OS=Pongo abelii GN=CWC22 PE=2 SV=1)

HSP 1 Score: 694.1 bits (1790), Expect = 1.8e-198
Identity = 351/696 (50.43%), Postives = 487/696 (69.97%), Query Frame = 1

Query: 107 HDRRQR-ETENENKNYDRRLDHDSKDERDKELLRKSDHRRNRTDSDRERRHSKHDYKHNN 166
           HDRR+   +   N + + R +   +  RD++     D+ R    SD E  HS+  + +++
Sbjct: 14  HDRRENLNSYQRNSSPEDRYEEQERSPRDRDYF---DYSR----SDYE--HSRRGHSYDS 73

Query: 167 FQDQRDKEASGRREDRRNIGEKEKVGTGT---------IDHLQPHRQEKQTENNLNPDSS 226
             + R+++   RRE  R+   K    + +         +    P + E  T+   +    
Sbjct: 74  SMESRNRDREKRRERERDTDRKRSRKSPSPGRRNPETSVTQSSPAQDEPATKKKKDELDP 133

Query: 227 NLGKSGGVYIPPFKLARMMKEVQDKSSIEYQRLTWDALRKSINGLVNKVNATNIKNIIPE 286
            L ++GG YIPP KL  M +++ DK+S+ YQR++W+AL+KSINGL+NKVN +NI  II E
Sbjct: 134 LLTRTGGAYIPPAKLRMMQEQITDKNSLAYQRMSWEALKKSINGLINKVNISNISIIIQE 193

Query: 287 LFAENLIRGRGLFCRSCMKSQMASPGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRA 346
           L  EN++RGRGL  RS +++Q ASP FT V+AALVA++N+KFP++G+L+L+R++L  ++ 
Sbjct: 194 LLQENIVRGRGLLSRSVLQAQSASPIFTHVYAALVAIINSKFPQIGELILKRLILNFRKG 253

Query: 347 YKRNDKPQLLAAVKFIAHLVNQQVAHEIIALELLTVLLENPTDDSVEVAVGFVTECGSIL 406
           Y+RNDK   L A KF+AHL+NQ VAHE++ LE+LT+LLE PTDDSVEVA+GF+ ECG  L
Sbjct: 254 YRRNDKQLCLTASKFVAHLINQNVAHEVLCLEMLTLLLERPTDDSVEVAIGFLKECGLKL 313

Query: 407 KDLSPKGLHGIFERFRGILHEGEIDKRVQFLIEGLFAIRKAKFEGYPAVRPELDLVEQED 466
             +SP+G++ IF R R ILHE EIDKRVQ++IE +FA+RK  F+ +P +   LDLVE++D
Sbjct: 314 TQVSPRGINAIFGRLRNILHESEIDKRVQYMIEVMFAVRKDGFKDHPIILEGLDLVEEDD 373

Query: 467 QLTHEISLQEEIDPEITLDIFKSDPNFLENEKRYEDLKKNILGEESEDEEDRSDAGSDES 526
           Q TH + L+++ +PE  L++FK DPNF+ENE++Y+ +KK IL E   D     DAGS E 
Sbjct: 374 QFTHMLPLEDDYNPEDVLNVFKMDPNFMENEEKYKAIKKEILDEGDTDSNTDQDAGSSEE 433

Query: 527 EDEDEEDESEEEDE-EQMQINDETETNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPG 586
           ++E+EE+E EE++E +++ I+D+TE NLV+ RRTIYL I SS+DFEE  HKLLK++    
Sbjct: 434 DEEEEEEEGEEDEEGQKVTIHDKTEINLVSFRRTIYLAIQSSLDFEECAHKLLKMEFPES 493

Query: 587 QEIELCVMLLECCSQERTYLRYYGLLGQRFCKINKVYQENFDKCFVQQYSMIHRLETNKL 646
           Q  ELC M+L+CC+Q+RTY +++GLL  RFC + K Y E+F+  F +QY  IHRLETNKL
Sbjct: 494 QTKELCNMILDCCAQQRTYEKFFGLLAGRFCMLKKEYMESFEGIFKEQYDTIHRLETNKL 553

Query: 647 RNVAKFFAHLLGTDALPWHVLSYIRLTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLT 706
           RNVAK FAHLL TD+LPW VL  I+L+EE TTSSSRIF+KI FQEL E++G+  LN RL 
Sbjct: 554 RNVAKMFAHLLYTDSLPWSVLECIKLSEETTTSSSRIFVKIFFQELCEYMGLPKLNARLK 613

Query: 707 DPTMQDSFESIFPRDNPKNTRFSINFFTSIGLGGLTENLREYLKNMPRLIMQQQKPV--S 766
           D T+Q  FE + PRDNP+NTRF+INFFTSIGLGGLT+ LRE+LKN P++I+ Q+  V  +
Sbjct: 614 DETLQPFFEGLLPRDNPRNTRFAINFFTSIGLGGLTDELREHLKNTPKVIVAQKPDVEQN 673

Query: 767 DSDEESQSSDSSDSDSSSSESKSDSEDSEIEDRRRE 790
            S   S SS SS S+S SS+S SDS DS  E    E
Sbjct: 674 KSSPSSSSSASSSSESDSSDSDSDSSDSSSESSSEE 700

HSP 2 Score: 45.4 bits (106), Expect = 3.3e-03
Identity = 33/114 (28.95%), Postives = 50/114 (43.86%), Query Frame = 1

Query: 21  TSRDNDRQDRDKRHQDKNLHREVERGDKKKSY----ESFDEGEQRTERTKSRHDRDRRHH 80
           + R+N  +  + R Q+ ++  E + GD KK       SF E E+ T R K   +  R+  
Sbjct: 792 SERNNYSRVANDRDQEMHIDLENKHGDPKKKRGERRNSFSENEKHTHRNKDSENFRRKDR 851

Query: 81  DEKRHSRGQERENRKESYQSSDED------ERKNRRHDRRQRETENENKNYDRR 125
            + R        NR+ S   SDED      ER+  +  R   ++    KN DRR
Sbjct: 852 SKSREM------NRRHSGSRSDEDRYQNGAERRWEKSSRYSEQSRESKKNQDRR 899

HSP 3 Score: 36.2 bits (82), Expect = 2.0e+00
Identity = 27/123 (21.95%), Postives = 46/123 (37.40%), Query Frame = 1

Query: 49  KKSYESFDEGEQRTERTKSRHDRDRRHHDEKRHSRGQERENRKESYQSSDEDERKNRRHD 108
           K S  S       +E   S  D D      +  S   +  +      +S  D RK     
Sbjct: 665 KSSPSSSSSASSSSESDSSDSDSDSSDSSSESSSEESDSSSISSHSSASANDVRKKGHGK 724

Query: 109 RRQRETENENKNYDRRLDHDSKDERDKELLRKSDHRRNRTDSDRERRHSKHDYKHNNFQD 168
            R +E +        +L  + +    K+  R+ +H    T ++RERR  KH  +++   +
Sbjct: 725 TRSKEVD--------KLIRNQQTNDRKQKERRQEHGHQETRTERERRSEKHRDQNSRDSN 779

Query: 169 QRD 172
            RD
Sbjct: 785 WRD 779

BLAST of Cucsa.065410 vs. Swiss-Prot
Match: CWC22_MOUSE (Pre-mRNA-splicing factor CWC22 homolog OS=Mus musculus GN=Cwc22 PE=1 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 2.3e-198
Identity = 360/696 (51.72%), Postives = 487/696 (69.97%), Query Frame = 1

Query: 103 KNRRHDRRQRETENENKNYDRRLDHDSKDERDKELLRKSDHRRNRTDSDRERRHSKHDYK 162
           K+  H+RR  ET +  +      D  ++ ER       SD+ R    SD ER  S+  Y 
Sbjct: 9   KSSGHNRR--ETHSSYRRSSSPEDRYTEQERSPRDRGYSDYSR----SDYER--SRRGYS 68

Query: 163 HNNFQDQRDKEASGRREDRRNIGEKEKVGTGTIDHLQPHRQEKQTENNLNPDSSN----- 222
           +++  + R ++   RRE  R+   +++          P R   Q+     P         
Sbjct: 69  YDDSMESRSRDREKRRERERDADHRKRSRKSPSPDRSPARGGGQSSPQEEPTWKKKKDEL 128

Query: 223 ---LGKSGGVYIPPFKLARMMKEVQDKSSIEYQRLTWDALRKSINGLVNKVNATNIKNII 282
              L ++GG YIPP KL  M +++ DKSS+ YQR++W+AL+KSINGL+NKVN +NI  II
Sbjct: 129 DPLLTRTGGAYIPPAKLRMMQEQITDKSSLAYQRMSWEALKKSINGLINKVNISNISIII 188

Query: 283 PELFAENLIRGRGLFCRSCMKSQMASPGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLK 342
            EL  EN++RGRGL  RS +++Q ASP FT V+AALVA++N+KFP++G+L+L+R++L  +
Sbjct: 189 QELLQENIVRGRGLLSRSVLQAQSASPIFTHVYAALVAIINSKFPQIGELILKRLILNFR 248

Query: 343 RAYKRNDKPQLLAAVKFIAHLVNQQVAHEIIALELLTVLLENPTDDSVEVAVGFVTECGS 402
           + Y+RNDK   L A KF+AHL+NQ VAHE++ LE+LT+LLE PTDDSVEVA+GF+ ECG 
Sbjct: 249 KGYRRNDKQLCLTASKFVAHLINQNVAHEVLCLEMLTLLLERPTDDSVEVAIGFLKECGL 308

Query: 403 ILKDLSPKGLHGIFERFRGILHEGEIDKRVQFLIEGLFAIRKAKFEGYPAVRPELDLVEQ 462
            L  +SP+G++ IFER R ILHE EIDKRVQ++IE +FA+RK  F+ +P +   LDLVE+
Sbjct: 309 KLTQVSPRGINAIFERLRNILHESEIDKRVQYMIEVMFAVRKDGFKDHPVILEGLDLVEE 368

Query: 463 EDQLTHEISLQEEIDPEITLDIFKSDPNFLENEKRYEDLKKNILGEESEDEEDRSDAGSD 522
           +DQ TH + L+++ +PE  L++FK DPNF+ENE++Y+ +KK IL E   D +  +D G+ 
Sbjct: 369 DDQFTHMLPLEDDYNPEDVLNVFKMDPNFMENEEKYKAIKKEILDE--GDSDSNTDQGAG 428

Query: 523 ESEDEDEEDESEE-EDEE---QMQINDETETNLVNLRRTIYLTIMSSVDFEEAGHKLLKI 582
            SEDE+EEDE EE EDEE   ++ I+D+TE NLV+ RRTIYL I SS+DFEE  HKLLK+
Sbjct: 429 SSEDEEEEDEEEEGEDEEGGQKVTIHDKTEINLVSFRRTIYLAIQSSLDFEECAHKLLKM 488

Query: 583 KLEPGQEIELCVMLLECCSQERTYLRYYGLLGQRFCKINKVYQENFDKCFVQQYSMIHRL 642
           +    Q  ELC M+L+CC+Q+RTY +++GLL  RFC + K Y E+F+  F +QY  IHRL
Sbjct: 489 EFAESQTKELCNMILDCCAQQRTYEKFFGLLAGRFCMLKKEYMESFESIFKEQYDTIHRL 548

Query: 643 ETNKLRNVAKFFAHLLGTDALPWHVLSYIRLTEEDTTSSSRIFIKILFQELSEHLGIRLL 702
           ETNKLRNVAK FAHLL TD+LPW VL  I+L+EE TTSSSRIF+KI FQEL E++G+  L
Sbjct: 549 ETNKLRNVAKMFAHLLYTDSLPWSVLECIKLSEETTTSSSRIFVKIFFQELCEYMGLPKL 608

Query: 703 NERLTDPTMQDSFESIFPRDNPKNTRFSINFFTSIGLGGLTENLREYLKNMPRLIM---- 762
           N RL D T+Q  FE + PRDNP+NTRF+INFFTSIGLGGLT+ LRE+LKN P++I+    
Sbjct: 609 NARLKDETLQPFFEGLLPRDNPRNTRFAINFFTSIGLGGLTDELREHLKNTPKVIVAQKP 668

Query: 763 --QQQKP--VSDSDEESQSSDSSDSDSSSSESKSDS 779
             +Q+KP   S S E S +SDSSDS+S SSES S+S
Sbjct: 669 EAEQKKPALTSSSSESSSASDSSDSESDSSESSSES 694

BLAST of Cucsa.065410 vs. TrEMBL
Match: A0A0A0L1Y4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G290770 PE=4 SV=1)

HSP 1 Score: 1588.5 bits (4112), Expect = 0.0e+00
Identity = 795/797 (99.75%), Postives = 796/797 (99.87%), Query Frame = 1

Query: 1   MDRKHSRESSDEDEVKGNRRTSRDNDRQDRDKRHQDKNLHREVERGDKKKSYESFDEGEQ 60
           MDRK+SRESSDEDEVKGNRRTSRDNDRQDRDKRHQDKNLHREVERGDKKKSYESFDEGEQ
Sbjct: 1   MDRKYSRESSDEDEVKGNRRTSRDNDRQDRDKRHQDKNLHREVERGDKKKSYESFDEGEQ 60

Query: 61  RTERTKSRHDRDRRHHDEKRHSRGQERENRKESYQSSDEDERKNRRHDRRQRETENENKN 120
           RTER KSRHDRDRRHHDEKRHSRGQERENRKESYQSSDEDERKNRRHDRRQRETENENKN
Sbjct: 61  RTERNKSRHDRDRRHHDEKRHSRGQERENRKESYQSSDEDERKNRRHDRRQRETENENKN 120

Query: 121 YDRRLDHDSKDERDKELLRKSDHRRNRTDSDRERRHSKHDYKHNNFQDQRDKEASGRRED 180
           YDRRLDHDSKDERDKELLRKSDHRRNRTDSDRERRHSKHDYKHNNFQDQRDKEASGRRED
Sbjct: 121 YDRRLDHDSKDERDKELLRKSDHRRNRTDSDRERRHSKHDYKHNNFQDQRDKEASGRRED 180

Query: 181 RRNIGEKEKVGTGTIDHLQPHRQEKQTENNLNPDSSNLGKSGGVYIPPFKLARMMKEVQD 240
           RRNIGEKEKVGTGTIDHLQPHRQEKQTENNLNPDSSNLGKSGGVYIPPFKLARMMKEVQD
Sbjct: 181 RRNIGEKEKVGTGTIDHLQPHRQEKQTENNLNPDSSNLGKSGGVYIPPFKLARMMKEVQD 240

Query: 241 KSSIEYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLFCRSCMKSQMAS 300
           KSSIEYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLFCRSCMKSQMAS
Sbjct: 241 KSSIEYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLFCRSCMKSQMAS 300

Query: 301 PGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQV 360
           PGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQV
Sbjct: 301 PGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQV 360

Query: 361 AHEIIALELLTVLLENPTDDSVEVAVGFVTECGSILKDLSPKGLHGIFERFRGILHEGEI 420
           AHEIIALELLTVLLENPTDDSVEVAVGFVTECGSILKDLSPKGLHGIFERFRGILHEGEI
Sbjct: 361 AHEIIALELLTVLLENPTDDSVEVAVGFVTECGSILKDLSPKGLHGIFERFRGILHEGEI 420

Query: 421 DKRVQFLIEGLFAIRKAKFEGYPAVRPELDLVEQEDQLTHEISLQEEIDPEITLDIFKSD 480
           DKRVQFLIEGLFAIRKAKFEGYPAVRPELDLVEQEDQLTHEISLQEEIDPEITLDIFKSD
Sbjct: 421 DKRVQFLIEGLFAIRKAKFEGYPAVRPELDLVEQEDQLTHEISLQEEIDPEITLDIFKSD 480

Query: 481 PNFLENEKRYEDLKKNILGEESEDEEDRSDAGSDESEDEDEEDESEEEDEEQMQINDETE 540
           PNFLENEKRYEDLKKNILGEESEDEEDRSDAGSDESEDEDEEDESEEEDEEQMQINDETE
Sbjct: 481 PNFLENEKRYEDLKKNILGEESEDEEDRSDAGSDESEDEDEEDESEEEDEEQMQINDETE 540

Query: 541 TNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEIELCVMLLECCSQERTYLRYYGL 600
           TNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEIELCVMLLECCSQERTYLRYYGL
Sbjct: 541 TNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEIELCVMLLECCSQERTYLRYYGL 600

Query: 601 LGQRFCKINKVYQENFDKCFVQQYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVLSYIR 660
           LGQRFCKINKVYQENFDKCFVQQYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVLSYIR
Sbjct: 601 LGQRFCKINKVYQENFDKCFVQQYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVLSYIR 660

Query: 661 LTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLTDPTMQDSFESIFPRDNPKNTRFSIN 720
           LTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLTDPTMQDSFESIFPRDNPKNTRFSIN
Sbjct: 661 LTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLTDPTMQDSFESIFPRDNPKNTRFSIN 720

Query: 721 FFTSIGLGGLTENLREYLKNMPRLIMQQQKPVSDSDEESQSSDSSDSDSSSSESKSDSED 780
           FFTSIGLGGLTENLREYLKNMPRLIMQQQKPVSDSDEESQSSDSSDSDSSSSESKSDSED
Sbjct: 721 FFTSIGLGGLTENLREYLKNMPRLIMQQQKPVSDSDEESQSSDSSDSDSSSSESKSDSED 780

Query: 781 SEIEDRRREKKRRKTRR 798
           SEIEDRRREKKRRKTRR
Sbjct: 781 SEIEDRRREKKRRKTRR 797

BLAST of Cucsa.065410 vs. TrEMBL
Match: W9SDK4_9ROSA (Pre-mRNA-splicing factor CWC22-like protein OS=Morus notabilis GN=L484_000596 PE=4 SV=1)

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 593/837 (70.85%), Postives = 689/837 (82.32%), Query Frame = 1

Query: 2   DRKHSRESSDEDEVKGNRR------TSRDNDRQDRDKRHQ--------DKNLHREVE--- 61
           DR H R SS  D    +RR       S D+DR +R KRH+        DK+  RE +   
Sbjct: 20  DRHHHRSSSRRDSRSYDRRGRGDESDSSDSDRGNRRKRHEGRESRHSDDKDKERETDARD 79

Query: 62  RG--------DKKKSYESFDEGEQRTERTKSRHDRDRRHHDEKRHSRGQERENR--KESY 121
           RG        D++ S  S+DE +++ E+ +   +R+R    +  H + +  E+   +ES+
Sbjct: 80  RGRWRASGQEDRRPSQRSYDEDDEKVEKERGLRERERARQQDNGHKKRESEEDDMVRESH 139

Query: 122 QSSDEDERKNRR------------HDRRQRETENENKNYDRRLDHDSKDERDKELLRKSD 181
           QSS+ED++K +R            +D R +ETE   +N DR   H  +DE D+E  R +D
Sbjct: 140 QSSEEDDQKIKRDRREKEKTRRYDNDGRTQETEYGYRNRDRHSRHYLEDEDDREEQR-TD 199

Query: 182 HRRNRTDSDRERRHSKHDYKHNNFQDQRDKEASGRREDRRNIGEKEKVGTGTIDHLQPHR 241
            +R+R   +++RRH + D +  + ++ RDK+   +RED +++ ++       +++ +P  
Sbjct: 200 RKRDRKGDNKQRRHRREDER--DLRNHRDKDEKEQREDSQSLRQRSDGNGKLLENSKPEG 259

Query: 242 QEKQTENNLNPDSSNLGKSGGVYIPPFKLARMMKEVQDKSSIEYQRLTWDALRKSINGLV 301
           Q    ENNL+ D+SNLGKSGGVY+PPFKLARMMKEVQDKSS+EYQRLTWDALRKSINGLV
Sbjct: 260 QST-VENNLSADASNLGKSGGVYVPPFKLARMMKEVQDKSSVEYQRLTWDALRKSINGLV 319

Query: 302 NKVNATNIKNIIPELFAENLIRGRGLFCRSCMKSQMASPGFTDVFAALVAVVNTKFPEVG 361
           NKVNATNIKNIIPELF+ENLIRGRGLFCRSCMKSQMASPGFTDVFAALVAVVNTKFPEVG
Sbjct: 320 NKVNATNIKNIIPELFSENLIRGRGLFCRSCMKSQMASPGFTDVFAALVAVVNTKFPEVG 379

Query: 362 DLLLRRIVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQVAHEIIALELLTVLLENPTDDSV 421
           +L+LRRIVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQVAHEI+ALELLTVLLENPTDDSV
Sbjct: 380 ELILRRIVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQVAHEIVALELLTVLLENPTDDSV 439

Query: 422 EVAVGFVTECGSILKDLSPKGLHGIFERFRGILHEGEIDKRVQFLIEGLFAIRKAKFEGY 481
           EVAVGFVTECGSIL+DLSPKGLHGIFERFRGILHEGEIDKRVQFLIEGLFAIRKAKF+GY
Sbjct: 440 EVAVGFVTECGSILQDLSPKGLHGIFERFRGILHEGEIDKRVQFLIEGLFAIRKAKFQGY 499

Query: 482 PAVRPELDLVEQEDQLTHEISLQEEIDPEITLDIFKSDPNFLENEKRYEDLKKNILGEES 541
           PAVRPELDLVEQEDQLTHEISLQEEIDPEITLD+FK DPN+ ENEKRYE+L+KNILGEES
Sbjct: 500 PAVRPELDLVEQEDQLTHEISLQEEIDPEITLDLFKPDPNYSENEKRYEELRKNILGEES 559

Query: 542 EDEEDRSDAGSDESEDEDEEDESEEEDEEQMQINDETETNLVNLRRTIYLTIMSSVDFEE 601
           EDEE  SD  SD +ED+D++D+SEEE++E+MQI DETETNLVNLRRTIYLTIMSSVDFEE
Sbjct: 560 EDEEG-SDTSSD-AEDDDDDDDSEEEEDEEMQIKDETETNLVNLRRTIYLTIMSSVDFEE 619

Query: 602 AGHKLLKIKLEPGQEIELCVMLLECCSQERTYLRYYGLLGQRFCKINKVYQENFDKCFVQ 661
           AGHKLLKIKLEPGQE+ELC+MLLECCSQERTYLRYYGLLGQRFC INKV+QENF+KCFVQ
Sbjct: 620 AGHKLLKIKLEPGQEMELCIMLLECCSQERTYLRYYGLLGQRFCMINKVHQENFEKCFVQ 679

Query: 662 QYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVLSYIRLTEEDTTSSSRIFIKILFQELS 721
           QYSMIHRLETNKLRNVAKFFAHLLGTDALPWH L+YIRLTEEDTTSSSRIFIKILFQELS
Sbjct: 680 QYSMIHRLETNKLRNVAKFFAHLLGTDALPWHCLAYIRLTEEDTTSSSRIFIKILFQELS 739

Query: 722 EHLGIRLLNERLTDPTMQDSFESIFPRDNPKNTRFSINFFTSIGLGGLTENLREYLKNMP 781
           EHLG+RLLNERL DP+MQDSFESIFP+DNPKNTRF+INFFTSIGLGG+TENLREYLKNMP
Sbjct: 740 EHLGLRLLNERLNDPSMQDSFESIFPKDNPKNTRFAINFFTSIGLGGITENLREYLKNMP 799

Query: 782 RLIMQQQKPV--SDSDEESQSSDSSDSDSSSSESKSDSEDSEIEDRRREKKRRKTRR 798
            LIMQQQK V  S+SD+E+ SS SSDSDS+SSES+  S   E + ++ EK+R++ RR
Sbjct: 800 NLIMQQQKHVSESESDDEAGSSGSSDSDSASSESEESSSSDESKSKKDEKRRKRRRR 850

BLAST of Cucsa.065410 vs. TrEMBL
Match: K7L9V4_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_08G302400 PE=4 SV=1)

HSP 1 Score: 1126.3 bits (2912), Expect = 0.0e+00
Identity = 587/805 (72.92%), Postives = 658/805 (81.74%), Query Frame = 1

Query: 2   DRKHSRESSDEDEVKGNRRTSRDNDRQDRDKRHQDKNLHREVERGDK--KKSYESFDEGE 61
           +RK S  S +E ++    R   +     R +R  D    R+ E  D+  ++S+   D+ E
Sbjct: 75  ERKESESSEEEGQIVSKERIREEKKEDARLQRRHDGRGGRDNEGDDRDGRESHRGNDQDE 134

Query: 62  QR--TERTKSRH-------DRDRRHHDEKRHSRGQERENRKESYQSSDEDERKNRRHDRR 121
           +R  TE  +  H       D DR+  +++R     E E+ +E  + S+ D   NR  DRR
Sbjct: 135 KRGETESHRLHHGDRWRGGDEDRQKINDRRGRHDSEEEDYRELARKSERDRDNNRDRDRR 194

Query: 122 QRETENENKNYDRRLDHDSKDERDKELLRKSDHRRNRT-DSDRERRHSKHDYKHNNFQDQ 181
           QR  +  +   DRR+ H+S++E  +EL RK D  R+R  D DR RR  K      N    
Sbjct: 195 QRVEKGGDNERDRRMRHNSEEEDARELARKLDRDRDRDRDRDRRRRVEKDGGSERN---H 254

Query: 182 RDKEASGRREDRRNIGEKEKVGTGTIDHLQPHRQEKQTENNLNPDSSNLGKSGGVYIPPF 241
           R+K+ S R+E+ R   +KE      ++  +P RQ      NLN D+SNLGKSGGVYIPPF
Sbjct: 255 REKDVSKRQEESR---KKEDGNGRMVEDEKPQRQATIPGGNLNGDASNLGKSGGVYIPPF 314

Query: 242 KLARMMKEVQDKSSIEYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLF 301
           K+A MMKEVQDKSS+EYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLF
Sbjct: 315 KMAMMMKEVQDKSSVEYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLF 374

Query: 302 CRSCMKSQMASPGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAV 361
           CRSCMKSQMASPGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAV
Sbjct: 375 CRSCMKSQMASPGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAV 434

Query: 362 KFIAHLVNQQVAHEIIALELLTVLLENPTDDSVEVAVGFVTECGSILKDLSPKGLHGIFE 421
           KFIAHLVNQQ AHEIIALELLTVLLE PTDDSVEVAVGFVTECGSIL+DLSPKGLHGIFE
Sbjct: 435 KFIAHLVNQQAAHEIIALELLTVLLEKPTDDSVEVAVGFVTECGSILQDLSPKGLHGIFE 494

Query: 422 RFRGILHEGEIDKRVQFLIEGLFAIRKAKFEGYPAVRPELDLVEQEDQLTHEISLQEEID 481
           RFRGILHEGEIDKRVQFLIEGLFAIRKAKF+GYPAVRPELDLVEQEDQ+THE+SL EEID
Sbjct: 495 RFRGILHEGEIDKRVQFLIEGLFAIRKAKFQGYPAVRPELDLVEQEDQITHEVSLDEEID 554

Query: 482 PEITLDIFKSDPNFLENEKRYEDLKKNILGEESEDEEDRSDAGSDESEDEDEEDESEEED 541
           PEI+LDIFK DPNFLENEKRYE+LKK++LGEESED+E+  D+ SD  +D+DE+DES+EE+
Sbjct: 555 PEISLDIFKPDPNFLENEKRYEELKKSMLGEESEDDEEGLDSESD--DDDDEDDESDEEE 614

Query: 542 EEQMQINDETETNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEIELCVMLLECCS 601
           EEQMQI DETETNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQE+ELC+MLLECCS
Sbjct: 615 EEQMQIKDETETNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEMELCIMLLECCS 674

Query: 602 QERTYLRYYGLLGQRFCKINKVYQENFDKCFVQQYSMIHRLETNKLRNVAKFFAHLLGTD 661
           QERTYLRYYGLLGQRFC INKV+QENF+KCFVQQYSMIHRLETNKLRNV KFFAHLLGTD
Sbjct: 675 QERTYLRYYGLLGQRFCMINKVHQENFEKCFVQQYSMIHRLETNKLRNVGKFFAHLLGTD 734

Query: 662 ALPWHVLSYIRLTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLTDPTMQDSFESIFPR 721
           ALPWHVLSYIRLTEEDTTSSSRIFIKILFQELSEHLGIRLLNERL DPTMQ+SFESIFP+
Sbjct: 735 ALPWHVLSYIRLTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLNDPTMQESFESIFPK 794

Query: 722 DNPKNTRFSINFFTSIGLGGLTENLREYLKNMPRLIMQQQKPVSDSDEESQSSDSSDSDS 781
           DNPKNTRF INFFTSIGLGGLTENLREYLKNMPRLIMQQQK VS+S+ + +S  S  SDS
Sbjct: 795 DNPKNTRFCINFFTSIGLGGLTENLREYLKNMPRLIMQQQKQVSESESDDESGSSDSSDS 854

Query: 782 SSSESKSDSEDSEIEDRRREKKRRK 795
            ++ S SDS  S+  DRRR K+RRK
Sbjct: 855 GTASSDSDSASSDESDRRRRKRRRK 871

BLAST of Cucsa.065410 vs. TrEMBL
Match: K7L9V4_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_08G302400 PE=4 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 5.1e-11
Identity = 78/274 (28.47%), Postives = 127/274 (46.35%), Query Frame = 1

Query: 1   MDRKHSRES-SDEDEVKGNRRTSRDNDRQDRDKR-----HQDKNLHREV-ERGD------ 60
           M R+HSR S S ED+V+  R +  +++ +DR  R     ++  + HR   E GD      
Sbjct: 1   MARRHSRSSESSEDDVRKRRGSESEDEERDRRYRRGGWSNKKSSGHRSSRENGDGSDSGE 60

Query: 61  ---------------KKKSYESFDEGE----QRTERTKSRHDRDRRHHDEK--RHSRGQE 120
                          +K+S  S +EG+    +R    K    R +R HD +  R + G +
Sbjct: 61  ERNGAKRDGKSKSRERKESESSEEEGQIVSKERIREEKKEDARLQRRHDGRGGRDNEGDD 120

Query: 121 RENRKESYQSSDEDERK-----NRRH--DRRQRETENENKNYDRRLDHDSKDERDKELLR 180
           R+ R ES++ +D+DE++     +R H  DR +   E+  K  DRR  HDS++E  +EL R
Sbjct: 121 RDGR-ESHRGNDQDEKRGETESHRLHHGDRWRGGDEDRQKINDRRGRHDSEEEDYRELAR 180

Query: 181 KSDH-RRNRTDSDRERRHSK-------HDYKHNNFQD---------QRDKEASGRREDRR 217
           KS+  R N  D DR +R  K          +HN+ ++          RD++    R+ RR
Sbjct: 181 KSERDRDNNRDRDRRQRVEKGGDNERDRRMRHNSEEEDARELARKLDRDRDRDRDRDRRR 240

HSP 2 Score: 1111.7 bits (2874), Expect = 0.0e+00
Identity = 588/817 (71.97%), Postives = 663/817 (81.15%), Query Frame = 1

Query: 2   DRKHSRESSDEDEV-------KGNRRTSRDNDRQDRDKRHQDKNLHREVER---GD---- 61
           D++ +R+ S+E E        K  +R  R     + D +H  ++  R+ ER   GD    
Sbjct: 66  DKRANRDRSEESERIHADNGGKDRQREKRPRSSDEEDGKHSYRDRRRKRERIHDGDDDGK 125

Query: 62  ---KKKSYESFDEGEQRTERTKSRHDRDRRHHDEKRHSRGQERENRKESYQSSDEDERKN 121
              +++   S DE + +      RH+RDR   D +  S+ + ++ ++ES +SSD+ E + 
Sbjct: 126 DRRREEGSRSSDEEDDKPSFRDRRHERDRIR-DGENDSKARRKDAQEESSESSDKSEEER 185

Query: 122 R----RHDRRQRETENENKNYDRRLDHDSKDERDKELLRKSDHRRNRTDSDRERRHSKHD 181
           R    R  +R R   ++  + DR  D   +++R +   R    R +  D D   R S  +
Sbjct: 186 RAKRDRSGKRDRRHRHDTGHRDRHNDKQREEDRYRNRDRWRHSRHDSDDDDDRGRRSHRN 245

Query: 182 YKHNNFQDQRDKEASGRREDRRNIGEKEKVGTGTIDHLQPHRQEKQTENNLNPDSSNLGK 241
            +  N   +RD +    R DR +   KEK   G I   QP  Q    + NLN D+SNLG+
Sbjct: 246 TERENRSQRRDNDNQPSRRDRDDNSFKEK-EEGEIKQKQPAFQ----QTNLNGDTSNLGR 305

Query: 242 SGGVYIPPFKLARMMKEVQDKSSIEYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAE 301
           SGGVYIPPFKLARMMKE QDKSS+EYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAE
Sbjct: 306 SGGVYIPPFKLARMMKETQDKSSVEYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAE 365

Query: 302 NLIRGRGLFCRSCMKSQMASPGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRN 361
           NLIRGRGLFCRSCMKSQMASPGFTDVFAALVAVVNTKFPEVG+LLLRRIVLQLKRAYKRN
Sbjct: 366 NLIRGRGLFCRSCMKSQMASPGFTDVFAALVAVVNTKFPEVGELLLRRIVLQLKRAYKRN 425

Query: 362 DKPQLLAAVKFIAHLVNQQVAHEIIALELLTVLLENPTDDSVEVAVGFVTECGSILKDLS 421
           DKPQLLAAVKF+AHLVNQQVAHEIIALELLTVLLENPTDDSVEVAVGFVTECGS+L+DLS
Sbjct: 426 DKPQLLAAVKFVAHLVNQQVAHEIIALELLTVLLENPTDDSVEVAVGFVTECGSLLQDLS 485

Query: 422 PKGLHGIFERFRGILHEGEIDKRVQFLIEGLFAIRKAKFEGYPAVRPELDLVEQEDQLTH 481
           PKGLHGIFERFRGILHEGEIDKRVQFLIEGLFAIRKAKF+GYPAVRPELDLVEQEDQLTH
Sbjct: 486 PKGLHGIFERFRGILHEGEIDKRVQFLIEGLFAIRKAKFQGYPAVRPELDLVEQEDQLTH 545

Query: 482 EISLQEEIDPEITLDIFKSDPNFLENEKRYEDLKKNILGEESEDEEDRSDAGSDESEDED 541
           EISLQ+EIDPEITLDIFK DP FLENEKRYE+LKK ILGE+SEDEE  SDAGS + ED++
Sbjct: 546 EISLQDEIDPEITLDIFKPDPQFLENEKRYEELKKTILGEDSEDEEG-SDAGSGDEEDDE 605

Query: 542 EEDESEEEDEEQMQINDETETNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEIEL 601
           ++DES+EEDEEQM+I DETETNL+NLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQE+EL
Sbjct: 606 DDDESDEEDEEQMKIQDETETNLINLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEMEL 665

Query: 602 CVMLLECCSQERTYLRYYGLLGQRFCKINKVYQENFDKCFVQQYSMIHRLETNKLRNVAK 661
           C+MLLECCSQERTYLRYYGLLGQRFC INKVYQENFDKCFVQQYSMIHRLETNKLRNVAK
Sbjct: 666 CIMLLECCSQERTYLRYYGLLGQRFCMINKVYQENFDKCFVQQYSMIHRLETNKLRNVAK 725

Query: 662 FFAHLLGTDALPWHVLSYIRLTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLTDPTMQ 721
           FFAHLLGTDALPWHVL+YIRLTEEDTTSSSRIFIKILFQELSEHLGIR LNERL+DPTMQ
Sbjct: 726 FFAHLLGTDALPWHVLAYIRLTEEDTTSSSRIFIKILFQELSEHLGIRRLNERLSDPTMQ 785

Query: 722 DSFESIFPRDNPKNTRFSINFFTSIGLGGLTENLREYLKNMPRLIMQQQKPVSDSD--EE 781
           DS ESIFPRDNPKNTRFSINFFTSIGLGG+TENLREYLKNMPRLIMQQQ+P S+S+  +E
Sbjct: 786 DSVESIFPRDNPKNTRFSINFFTSIGLGGITENLREYLKNMPRLIMQQQEPASESESGDE 845

Query: 782 SQSSDSSDSDSSSSESKSDSEDSEI-EDRRREKKRRK 795
           S SS SSDS+S+SSES+ +S+ S   E  RR KKRRK
Sbjct: 846 SGSSSSSDSESASSESEPESDSSSSDEGERRRKKRRK 875

BLAST of Cucsa.065410 vs. TrEMBL
Match: A0A0L9VAV7_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan09g079700 PE=4 SV=1)

HSP 1 Score: 1110.9 bits (2872), Expect = 0.0e+00
Identity = 595/800 (74.38%), Postives = 658/800 (82.25%), Query Frame = 1

Query: 2   DRKHSRESSDED-EVKGNRRTSRDNDRQDR---DKRHQDKNLHREVERGDKKKSYESFDE 61
           +RK   ESS+E+ ++   +R S +    +R   D   + +    E  RGD+  S     +
Sbjct: 78  ERKEKSESSEEEGQIVNKKRVSEEKGEDERLPQDYDGRGRRDAEEDSRGDRA-SRRVNGQ 137

Query: 62  GEQRTERTKSR-HDRDRRHHDEKRHSRGQERENRKESYQSSDEDERKNRRHDRRQRETEN 121
            E+R E+   R H RDR    E+  SR  +R  R +S +   E  RK+  +  R +  + 
Sbjct: 138 SERRGEKESHRLHHRDRWRGGEEGKSRIVDRRGRHDSEEDHIELTRKSDGYSDRDKRRKV 197

Query: 122 ENKNYDRRLDHDSKDERDKELLRKSDHRRNRTDSDRERRHSKHDYKHNNFQDQRDKEASG 181
           E K  +R   +DS+++ D+EL RKS+      DSDR RR  + D   +N ++ RDK  S 
Sbjct: 198 E-KGGNRHSRYDSEED-DRELARKSER-----DSDRRRRRPERD--DDNERNHRDKGVSK 257

Query: 182 RREDRRNIGEKEKVGTGTIDHLQPHRQEKQTENNLNPDSSNLGKSGGVYIPPFKLARMMK 241
           ++E+ R   +KE     T+    P RQ      NLN D+SNLGKSGGVYIPPFK+A MMK
Sbjct: 258 QQEESR---KKENGNGRTVRDANPQRQTTIPGGNLNGDASNLGKSGGVYIPPFKMAMMMK 317

Query: 242 EVQDKSSIEYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLFCRSCMKS 301
           EVQDKSS+EYQRLTWDALRKSINGLVNKVNATNIKNIIPELF+ENLIRGRGLFCRSCMKS
Sbjct: 318 EVQDKSSVEYQRLTWDALRKSINGLVNKVNATNIKNIIPELFSENLIRGRGLFCRSCMKS 377

Query: 302 QMASPGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAVKFIAHLV 361
           QMASPGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAVKFI HLV
Sbjct: 378 QMASPGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAVKFIGHLV 437

Query: 362 NQQVAHEIIALELLTVLLENPTDDSVEVAVGFVTECGSILKDLSPKGLHGIFERFRGILH 421
           NQQVAHEIIALELLTVLLE PTDDSVEVAVGFVTECGSIL+DLSPKGLHGIFERFRGILH
Sbjct: 438 NQQVAHEIIALELLTVLLEKPTDDSVEVAVGFVTECGSILQDLSPKGLHGIFERFRGILH 497

Query: 422 EGEIDKRVQFLIEGLFAIRKAKFEGYPAVRPELDLVEQEDQLTHEISLQEEIDPEITLDI 481
           EGEIDKRVQFLIEGLFAIRKAKF+GYPAVRPELDLVEQEDQLTHE+SL EEIDPEI+LDI
Sbjct: 498 EGEIDKRVQFLIEGLFAIRKAKFQGYPAVRPELDLVEQEDQLTHEVSLDEEIDPEISLDI 557

Query: 482 FKSDPNFLENEKRYEDLKKNILGEESEDEEDRSDAGSDESEDEDEEDESEEEDEEQMQIN 541
           FK DPNFLENEKRYE+LKK++LGEESEDEE+  DA   ES+DEDEEDES+EEDEEQMQI 
Sbjct: 558 FKPDPNFLENEKRYEELKKSMLGEESEDEEEGMDA---ESDDEDEEDESDEEDEEQMQIK 617

Query: 542 DETETNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEIELCVMLLECCSQERTYLR 601
           DETETNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQE+ELC+MLLECCSQERTYLR
Sbjct: 618 DETETNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEMELCIMLLECCSQERTYLR 677

Query: 602 YYGLLGQRFCKINKVYQENFDKCFVQQYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVL 661
           YYGLLGQRFC INKV+QENF+KCFVQQYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVL
Sbjct: 678 YYGLLGQRFCMINKVHQENFEKCFVQQYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVL 737

Query: 662 SYIRLTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLTDPTMQDSFESIFPRDNPKNTR 721
           SYIRLTEEDTTSSSRIFIKILFQELSEHLGIRLLNERL DPTMQ+SFESIFP+DNPKNTR
Sbjct: 738 SYIRLTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLNDPTMQESFESIFPKDNPKNTR 797

Query: 722 FSINFFTSIGLGGLTENLREYLKNMPRLIMQQQKPV--SDSDEESQSSDSSDSDSSSSES 781
           F INFFTSIGLGGLTENLREYLKNMPRLIMQQQK V  S+SD+ES SS SSDS SS S+S
Sbjct: 798 FCINFFTSIGLGGLTENLREYLKNMPRLIMQQQKQVSESESDDESGSSASSDSGSSDSDS 857

Query: 782 KSDSEDSEIEDRRREKKRRK 795
            S  E     DRR+ K+RRK
Sbjct: 858 TSSDESDAEHDRRQRKRRRK 861

BLAST of Cucsa.065410 vs. TAIR10
Match: AT1G80930.1 (AT1G80930.1 MIF4G domain-containing protein / MA3 domain-containing protein)

HSP 1 Score: 1011.1 bits (2613), Expect = 3.7e-295
Identity = 536/807 (66.42%), Postives = 635/807 (78.69%), Query Frame = 1

Query: 2   DRKHSRESSDE-DEVKGNRRTSR--DNDRQDRDKRHQDKNLHRE----VERGDKKKSYES 61
           DR  +  SSDE D++KGN++     D+D   R +R   K + ++      RG +  + + 
Sbjct: 127 DRVRADTSSDEEDDLKGNKKEPMEVDDDYGRRGRRRSPKVMEKQGRERSHRGSRVIADKP 186

Query: 62  FDEGEQRTERTKSRHDRDRRHHDEKRHSRGQERENRKE--SYQSSDEDERKNRRHDRRQR 121
            DE + R      R +  R+  D +     +E E R E    + +D       + DRR+R
Sbjct: 187 SDEEDDRQRSRGGRRESQRKRRDHRASDDDEEGEIRSERRGKEKNDRGSEGLLKRDRRER 246

Query: 122 ETENENKNYDRRLDHDSKDERDKELLRKSDHRRNRTDSDRERRHSKHDYKHNNFQDQRDK 181
           +  + ++N  RR + + KD            R  R D  R+ +  +H+ K+++   QRDK
Sbjct: 247 DLTDGHENGSRRRESERKD------------RSRRDDGVRDEKERRHNDKYDD--SQRDK 306

Query: 182 EASGRREDRRNIGEKEKVGTGTIDHLQPHRQEKQTENNLNPDSSN---LGKSGGVYIPPF 241
               R+ED R   EK+      I+  +P   E      LNP  +N   LGK+GGVYIPPF
Sbjct: 307 L---RKEDSRKREEKK------IEVPKPKLAE------LNPSENNAMALGKTGGVYIPPF 366

Query: 242 KLARMMKEVQDKSSIEYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLF 301
           KLARMMKEV+DKSS+EYQRLTWDALRKSINGLVNKVNA+NIKNIIPELFAENLIRGRGLF
Sbjct: 367 KLARMMKEVEDKSSVEYQRLTWDALRKSINGLVNKVNASNIKNIIPELFAENLIRGRGLF 426

Query: 302 CRSCMKSQMASPGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAV 361
           CRSCMKSQMASPGFTDVFAALVAV+N KFPEV +LLL+R+VLQLKRAYKRNDKPQLLAAV
Sbjct: 427 CRSCMKSQMASPGFTDVFAALVAVINAKFPEVAELLLKRVVLQLKRAYKRNDKPQLLAAV 486

Query: 362 KFIAHLVNQQVAHEIIALELLTVLLENPTDDSVEVAVGFVTECGSILKDLSPKGLHGIFE 421
           KFIAHLVNQQVA EIIALEL+T+LL +PTDDSVEVAVGFVTECG++L+D+SP+GL+GIFE
Sbjct: 487 KFIAHLVNQQVAEEIIALELVTILLGDPTDDSVEVAVGFVTECGAMLQDVSPRGLNGIFE 546

Query: 422 RFRGILHEGEIDKRVQFLIEGLFAIRKAKFEGYPAVRPELDLVEQEDQLTHEISLQEEID 481
           RFRGILHEGEIDKRVQ+LIE LFA RKAKF+G+PAVRPELDLVE++   +H++SL  EID
Sbjct: 547 RFRGILHEGEIDKRVQYLIESLFATRKAKFQGHPAVRPELDLVEEK--YSHDLSLDHEID 606

Query: 482 PEITLDIFKSDPNFLENEKRYEDLKKNILGEESEDEEDRSDAGSDESEDEDEEDESEEED 541
           PE  LDIFK DP+F+ENEK+YE LKK +LG+E  ++ED SDA S+  ++++EEDES+EED
Sbjct: 607 PETALDIFKPDPDFVENEKKYEALKKELLGDEESEDEDGSDASSE--DNDEEEDESDEED 666

Query: 542 EEQMQINDETETNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEIELCVMLLECCS 601
           EEQM+I DETETNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQE+ELC+MLLECCS
Sbjct: 667 EEQMRIRDETETNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEMELCIMLLECCS 726

Query: 602 QERTYLRYYGLLGQRFCKINKVYQENFDKCFVQQYSMIHRLETNKLRNVAKFFAHLLGTD 661
           QERTYLRYYGLLGQRFC INK++QENF+KCFVQQYSMIHRLETNKLRNVAKFFAHLLGTD
Sbjct: 727 QERTYLRYYGLLGQRFCMINKIHQENFEKCFVQQYSMIHRLETNKLRNVAKFFAHLLGTD 786

Query: 662 ALPWHVLSYIRLTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLTDPTMQDSFESIFPR 721
           ALPWHVL+YIRLTEEDTTSSSRIFIKILFQELSEHLGIRLLNERL DPTMQ+S ESIFP+
Sbjct: 787 ALPWHVLAYIRLTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLQDPTMQESLESIFPK 846

Query: 722 DNPKNTRFSINFFTSIGLGGLTENLREYLKNMPRLIMQQQKPVSDSDEESQS-SDSSDSD 781
           DNPKNTRF+INFFTSIGLGG+TENLREYLKNMP LIMQ+QK V++S+  S S SDSSDS+
Sbjct: 847 DNPKNTRFAINFFTSIGLGGITENLREYLKNMPSLIMQRQKQVAESESGSSSGSDSSDSE 900

Query: 782 SSSSESKSDSEDSEIEDRRREKKRRKT 796
           S S    S S  S+  DR + K+RR++
Sbjct: 907 SESESGSSSSSSSDESDREKRKRRRRS 900

BLAST of Cucsa.065410 vs. TAIR10
Match: AT1G52325.1 (AT1G52325.1 Initiation factor eIF-4 gamma, MA3)

HSP 1 Score: 101.3 bits (251), Expect = 2.9e-21
Identity = 49/75 (65.33%), Postives = 63/75 (84.00%), Query Frame = 1

Query: 664 EDTTSSSRIFIKILFQELSEHLGIRLLNERLTDPTMQDSFESIFPRDNPKNTRFSINFFT 723
           ED+TSSS IF+K LF +LSE L I+LLNE+L DPTM+++FESIFP+D+ KNT FSI FFT
Sbjct: 46  EDSTSSSLIFLKTLFLQLSELLRIKLLNEKLQDPTMEETFESIFPKDHRKNTLFSIIFFT 105

Query: 724 SIGLGGLTENLREYL 739
            IGLGG+T+ LR+ +
Sbjct: 106 KIGLGGITQTLRQLI 120

HSP 2 Score: 73.9 bits (180), Expect = 4.9e-13
Identity = 32/44 (72.73%), Postives = 40/44 (90.91%), Query Frame = 1

Query: 555 MSSVDFEEAGHKLLKIKLEPGQEIELCVMLLECCSQERTYLRYY 599
           MSS+DFEEAGHKLLKI+LE GQ +ELCVM+LECC++E+TY  +Y
Sbjct: 1   MSSLDFEEAGHKLLKIRLEQGQGMELCVMVLECCTEEKTYRSFY 44

BLAST of Cucsa.065410 vs. TAIR10
Match: AT5G53440.1 (AT5G53440.1 unknown protein)

HSP 1 Score: 81.3 bits (199), Expect = 3.1e-15
Identity = 59/194 (30.41%), Postives = 95/194 (48.97%), Query Frame = 1

Query: 3   RKHSRESSDEDEVKGNRRTSRDNDRQDRDKRHQDKNLHREVERGDKKKSYESFDEGEQRT 62
           R   RE  +E+++K      RD DR  ++    D+ L R+  +  KK  ++  D G +  
Sbjct: 238 RDKYREDKEEEDIKQKGDKQRD-DRPTKEHLRSDEKLTRDESK--KKSKFQDNDHGHEPD 297

Query: 63  ERTKSRHDRDRRHHDEKRHSRGQERENRKESYQSSDEDERKNRRHDR-RQRETENENKNY 122
                 H+R+R + D  R S   ER+  +E  +  D D  ++R  DR R RE + + ++Y
Sbjct: 298 SELDGYHERER-NRDYDRESDRNERD--RERTRDRDRDYERDRDRDRDRDRERDRDRRDY 357

Query: 123 DRRLDHDSKDERDKELLRKSDHRRNRTDSDRERRHSKHDYKHNNFQDQRDKE-------- 182
           +    HD   +RD+   R  DH R+RT  DRE+  S+ DY H+  + + D+E        
Sbjct: 358 EHDRYHDRDWDRDRSRDRDRDHERDRT-HDREKDRSR-DYYHDGKRSKSDRERDNDRDVS 417

Query: 183 ----ASGRREDRRN 184
                SGR +DRR+
Sbjct: 418 RLDDQSGRYKDRRD 423

HSP 2 Score: 68.9 bits (167), Expect = 1.6e-11
Identity = 62/232 (26.72%), Postives = 97/232 (41.81%), Query Frame = 1

Query: 4   KHSRESSDEDEVKGNRRTSRDNDRQDRDKRHQDKNLHREVERGDKKKSYESFDEGEQRTE 63
           K SR   D D   G++     +D  DR     D  +     +G+K +     D+ E   E
Sbjct: 193 KRSRRKRD-DHGTGDKHHDNSDDVGDRVLTSGDDYIKDGKHKGEKSRDKYREDKEE---E 252

Query: 64  RTKSRHDRDRRHHDEKRHSRGQERENRKESYQSS---DEDERKNRRHDRRQRETENENKN 123
             K + D+ R     K H R  E+  R ES + S   D D       +         N++
Sbjct: 253 DIKQKGDKQRDDRPTKEHLRSDEKLTRDESKKKSKFQDNDHGHEPDSELDGYHERERNRD 312

Query: 124 YDRRLDHDSKDERDKELLRKSDHRRNR---TDSDRERRHSKHDYKHNNFQDQ---RDKEA 183
           YDR  D + +D R++   R  D+ R+R    D DRER   + DY+H+ + D+   RD+  
Sbjct: 313 YDRESDRNERD-RERTRDRDRDYERDRDRDRDRDRERDRDRRDYEHDRYHDRDWDRDRSR 372

Query: 184 SGRRE-DRRNIGEKEKVGTGTIDHLQPHRQEKQTENNLNPDSSNLGKSGGVY 226
              R+ +R    ++EK  +    H    R +   E + + D S L    G Y
Sbjct: 373 DRDRDHERDRTHDREKDRSRDYYH-DGKRSKSDRERDNDRDVSRLDDQSGRY 418

HSP 3 Score: 55.1 bits (131), Expect = 2.3e-07
Identity = 56/193 (29.02%), Postives = 87/193 (45.08%), Query Frame = 1

Query: 1   MDRKHSRESS-----DEDEVKGNRRTSRDNDR---QDRDKRHQDKNLHREVERGDKKKS- 60
           +D  H RE +     + D  + +R  +RD DR   +DRD R +D++  R+ +R D +   
Sbjct: 297 LDGYHERERNRDYDRESDRNERDRERTRDRDRDYERDRD-RDRDRDRERDRDRRDYEHDR 356

Query: 61  YESFDEGEQRTERTKSRHDRDRRHHDEKRHSRGQERENRKESYQSSDEDERKNRRHDRRQ 120
           Y   D    R+      H+RDR H  EK  SR    + ++    S  + ER N   DR  
Sbjct: 357 YHDRDWDRDRSRDRDRDHERDRTHDREKDRSRDYYHDGKR----SKSDRERDN---DRDV 416

Query: 121 RETENENKNY-DRRLDHDSKDERD-KELLRKSDHRRNRTDSD-----RERRHSKHDYKHN 178
              ++++  Y DRR    S D +D ++++  S   R   D D     R+   S    ++ 
Sbjct: 417 SRLDDQSGRYKDRRDGRRSPDYQDYQDVITGSRSSRVEPDGDMTRPERQLSSSVVQEENG 476

BLAST of Cucsa.065410 vs. TAIR10
Match: AT5G17930.1 (AT5G17930.1 MIF4G domain-containing protein / MA3 domain-containing protein)

HSP 1 Score: 79.7 bits (195), Expect = 8.9e-15
Identity = 47/173 (27.17%), Postives = 90/173 (52.02%), Query Frame = 1

Query: 513 SDESEDEDEEDESEEEDEEQMQINDETETNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKL 572
           S+ +ED  E  ++E  + ++M    E +    + R+ I+  IMSS D+ +A  KLL++ L
Sbjct: 553 SNTAEDIAETMDAEVVEAQKMLKLAEAQRMNTDSRKAIFCVIMSSEDYIDAFEKLLRLDL 612

Query: 573 EPGQEIELCVMLLECCSQERTYLRYYGLLGQRFCKINKVYQENFDKCFVQQYSMIHRLET 632
              Q+ E+  +L+ECC QE+ + ++Y +L  + C+ +K ++     C    +  +  +  
Sbjct: 613 PGKQDREIMRVLVECCLQEKAFNKFYTVLASKLCEHDKNHKFTLQYCIWDHFKELESMSL 672

Query: 633 NKLRNVAKFFAHLLGTDALPWHVLSYIRLTEEDTTSSSRIF-IKILFQELSEH 685
            +  ++AKF A ++ T  L   VL  + L      +  RI   ++LF+ + EH
Sbjct: 673 QRSMHLAKFVAEIIVTFNLSLAVLKSVDLANPVELTPKRIMHFRMLFEAIFEH 725

HSP 2 Score: 32.7 bits (73), Expect = 1.2e+00
Identity = 25/91 (27.47%), Postives = 44/91 (48.35%), Query Frame = 1

Query: 475 DIFKSDPNFLEN------EKRYEDLKKNILGEESEDEED-----RSDAGSDESEDEDEED 534
           D+F+  P+ L++      + R +  KK    EE +D ED       D   +ESE  DEE 
Sbjct: 158 DLFEGLPSVLDSMGSELGDSRKKRKKKR--SEEKQDHEDVDELANEDLEHEESEFSDEES 217

Query: 535 ESE--------EEDEEQMQINDETETNLVNL 547
           E E           +++  +++E E++L+N+
Sbjct: 218 EEEPVGKRDRKRHKKKKKSVDEELESDLMNI 246

BLAST of Cucsa.065410 vs. TAIR10
Match: AT1G20920.1 (AT1G20920.1 P-loop containing nucleoside triphosphate hydrolases superfamily protein)

HSP 1 Score: 75.9 bits (185), Expect = 1.3e-13
Identity = 56/228 (24.56%), Postives = 108/228 (47.37%), Query Frame = 1

Query: 4   KHSRESSDEDEVKGN-RRTSRDNDRQDRDKRHQDKNLHREVERGDKK----KSYESFDEG 63
           K+  E  D  E + + +++ RD DR +  K+ +     RE +R  K+     S + +D  
Sbjct: 7   KYRSEDLDVVEEEADLKKSRRDRDRSNERKKDKGSEKRREKDRRKKRVKSSDSEDDYDRD 66

Query: 64  EQRTERTKSRHDRDRRHHDEKRHSRGQERENRKESYQSSDEDERKNRRHDRRQRETENEN 123
           +      +   +R+RR  D+ R  R   R  R++S  S D+ E ++ R  RR  E E  +
Sbjct: 67  DDEEREKRKEKERERRRRDKDRVKR---RSERRKSSDSEDDVEEEDERDKRRVNEKERGH 126

Query: 124 KNYDRRLDHDSKDERDKELLRKSDHRRNRTDSDRERRHSKHDYKHNNFQDQRDKEASGRR 183
           + ++R    D K +R++E   + D  R R + DRERR  + + +    +  +++E   R 
Sbjct: 127 REHERDRGKDRKRDRERE--ERKDKERER-EKDRERREREREEREK--ERVKERERRERE 186

Query: 184 EDRRNIGEKEKVGTGTIDHLQPHRQE---KQTENNLNPDSSNLGKSGG 224
           +  R+  E+EK      +  +   +E   +++++++  D     K GG
Sbjct: 187 DGERDRREREKERGSRRNRERERSREVGNEESDDDVKRDLKRRRKEGG 226

HSP 2 Score: 63.2 bits (152), Expect = 8.6e-10
Identity = 43/200 (21.50%), Postives = 85/200 (42.50%), Query Frame = 1

Query: 2   DRKHSRESSDEDEVKGNRRTSRDNDRQDRDKRHQDKNLHREVERGDKKKSYESFDEGEQR 61
           +++      + D  K  +R     +R+D+++  +     RE ER +++K     +  ++R
Sbjct: 118 EKERGHREHERDRGKDRKRDREREERKDKEREREKDRERREREREEREK-----ERVKER 177

Query: 62  TERTKSRHDRDRRHHDEKRHS-RGQERENRKESYQSSDEDERKNRRHDRRQRETENENKN 121
             R +   +RDRR  +++R S R +ERE  +E      +D+ K     RR+   E + K 
Sbjct: 178 ERREREDGERDRREREKERGSRRNRERERSREVGNEESDDDVKRDLKRRRKEGGERKEKE 237

Query: 122 YDRRLDHDSKDERDKELLRKSDHRRNRTDSDRERRHSKHDYKHNNFQDQRDKEASGRRED 181
            ++ +   S+ E   +     D+   +    RE        K +   ++R +     +E 
Sbjct: 238 REKSVGRSSRHEDSPKRKSVEDNGEKKEKKTREEELEDEQKKLDEEVEKRRRRVQEWQEL 297

Query: 182 RRNIGEKEKVGTGTIDHLQP 201
           +R   E E    G  D  +P
Sbjct: 298 KRKKEEAESESKGDADGNEP 312

HSP 3 Score: 40.8 bits (94), Expect = 4.6e-03
Identity = 35/142 (24.65%), Postives = 66/142 (46.48%), Query Frame = 1

Query: 435 RKAKFEGYPAVRPELDLVEQEDQLTHEISLQEE---IDPEITLDIFKSDPNFLENEKRYE 494
           R ++ E  P  +   D  E++++ T E  L++E   +D E+     +    + E +++ E
Sbjct: 239 RSSRHEDSPKRKSVEDNGEKKEKKTREEELEDEQKKLDEEVEKRRRRVQ-EWQELKRKKE 298

Query: 495 DLKKNILGEESEDEEDRSDAGSDESEDEDEEDESEEEDEEQMQINDET------ETNLVN 554
           + +    G+   +E     A + E E +DEE   EE+ E +M +++ET      +  +V+
Sbjct: 299 EAESESKGDADGNEPKAGKAWTLEGESDDEEGHPEEKSETEMDVDEETKPENDGDAKMVD 358

Query: 555 LRRTIYLTIMSS-----VDFEE 563
           L      T+  S     VD EE
Sbjct: 359 LENETAATVSESGGDGAVDEEE 379

HSP 4 Score: 30.8 bits (68), Expect = 4.7e+00
Identity = 19/56 (33.93%), Postives = 27/56 (48.21%), Query Frame = 1

Query: 485 ENEKRYEDLKKNILGEESEDEEDRSDAGSDESEDEDEEDESEEEDEEQMQINDETE 541
           E EKR E  +     E    ++DR    S+  +  D ED+ EEEDE   +  +E E
Sbjct: 70  EREKRKEKER-----ERRRRDKDRVKRRSERRKSSDSEDDVEEEDERDKRRVNEKE 120

HSP 5 Score: 30.4 bits (67), Expect = 6.2e+00
Identity = 13/29 (44.83%), Postives = 19/29 (65.52%), Query Frame = 1

Query: 769 SSSSESKSDSEDSEIEDRRREKKRRKTRR 798
           SS SE   D +D E  ++R+EK+R + RR
Sbjct: 56  SSDSEDDYDRDDDEEREKRKEKERERRRR 84

BLAST of Cucsa.065410 vs. NCBI nr
Match: gi|449448752|ref|XP_004142129.1| (PREDICTED: pre-mRNA-splicing factor CWC22 homolog [Cucumis sativus])

HSP 1 Score: 1588.5 bits (4112), Expect = 0.0e+00
Identity = 795/797 (99.75%), Postives = 796/797 (99.87%), Query Frame = 1

Query: 1   MDRKHSRESSDEDEVKGNRRTSRDNDRQDRDKRHQDKNLHREVERGDKKKSYESFDEGEQ 60
           MDRK+SRESSDEDEVKGNRRTSRDNDRQDRDKRHQDKNLHREVERGDKKKSYESFDEGEQ
Sbjct: 1   MDRKYSRESSDEDEVKGNRRTSRDNDRQDRDKRHQDKNLHREVERGDKKKSYESFDEGEQ 60

Query: 61  RTERTKSRHDRDRRHHDEKRHSRGQERENRKESYQSSDEDERKNRRHDRRQRETENENKN 120
           RTER KSRHDRDRRHHDEKRHSRGQERENRKESYQSSDEDERKNRRHDRRQRETENENKN
Sbjct: 61  RTERNKSRHDRDRRHHDEKRHSRGQERENRKESYQSSDEDERKNRRHDRRQRETENENKN 120

Query: 121 YDRRLDHDSKDERDKELLRKSDHRRNRTDSDRERRHSKHDYKHNNFQDQRDKEASGRRED 180
           YDRRLDHDSKDERDKELLRKSDHRRNRTDSDRERRHSKHDYKHNNFQDQRDKEASGRRED
Sbjct: 121 YDRRLDHDSKDERDKELLRKSDHRRNRTDSDRERRHSKHDYKHNNFQDQRDKEASGRRED 180

Query: 181 RRNIGEKEKVGTGTIDHLQPHRQEKQTENNLNPDSSNLGKSGGVYIPPFKLARMMKEVQD 240
           RRNIGEKEKVGTGTIDHLQPHRQEKQTENNLNPDSSNLGKSGGVYIPPFKLARMMKEVQD
Sbjct: 181 RRNIGEKEKVGTGTIDHLQPHRQEKQTENNLNPDSSNLGKSGGVYIPPFKLARMMKEVQD 240

Query: 241 KSSIEYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLFCRSCMKSQMAS 300
           KSSIEYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLFCRSCMKSQMAS
Sbjct: 241 KSSIEYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLFCRSCMKSQMAS 300

Query: 301 PGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQV 360
           PGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQV
Sbjct: 301 PGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQV 360

Query: 361 AHEIIALELLTVLLENPTDDSVEVAVGFVTECGSILKDLSPKGLHGIFERFRGILHEGEI 420
           AHEIIALELLTVLLENPTDDSVEVAVGFVTECGSILKDLSPKGLHGIFERFRGILHEGEI
Sbjct: 361 AHEIIALELLTVLLENPTDDSVEVAVGFVTECGSILKDLSPKGLHGIFERFRGILHEGEI 420

Query: 421 DKRVQFLIEGLFAIRKAKFEGYPAVRPELDLVEQEDQLTHEISLQEEIDPEITLDIFKSD 480
           DKRVQFLIEGLFAIRKAKFEGYPAVRPELDLVEQEDQLTHEISLQEEIDPEITLDIFKSD
Sbjct: 421 DKRVQFLIEGLFAIRKAKFEGYPAVRPELDLVEQEDQLTHEISLQEEIDPEITLDIFKSD 480

Query: 481 PNFLENEKRYEDLKKNILGEESEDEEDRSDAGSDESEDEDEEDESEEEDEEQMQINDETE 540
           PNFLENEKRYEDLKKNILGEESEDEEDRSDAGSDESEDEDEEDESEEEDEEQMQINDETE
Sbjct: 481 PNFLENEKRYEDLKKNILGEESEDEEDRSDAGSDESEDEDEEDESEEEDEEQMQINDETE 540

Query: 541 TNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEIELCVMLLECCSQERTYLRYYGL 600
           TNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEIELCVMLLECCSQERTYLRYYGL
Sbjct: 541 TNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEIELCVMLLECCSQERTYLRYYGL 600

Query: 601 LGQRFCKINKVYQENFDKCFVQQYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVLSYIR 660
           LGQRFCKINKVYQENFDKCFVQQYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVLSYIR
Sbjct: 601 LGQRFCKINKVYQENFDKCFVQQYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVLSYIR 660

Query: 661 LTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLTDPTMQDSFESIFPRDNPKNTRFSIN 720
           LTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLTDPTMQDSFESIFPRDNPKNTRFSIN
Sbjct: 661 LTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLTDPTMQDSFESIFPRDNPKNTRFSIN 720

Query: 721 FFTSIGLGGLTENLREYLKNMPRLIMQQQKPVSDSDEESQSSDSSDSDSSSSESKSDSED 780
           FFTSIGLGGLTENLREYLKNMPRLIMQQQKPVSDSDEESQSSDSSDSDSSSSESKSDSED
Sbjct: 721 FFTSIGLGGLTENLREYLKNMPRLIMQQQKPVSDSDEESQSSDSSDSDSSSSESKSDSED 780

Query: 781 SEIEDRRREKKRRKTRR 798
           SEIEDRRREKKRRKTRR
Sbjct: 781 SEIEDRRREKKRRKTRR 797

BLAST of Cucsa.065410 vs. NCBI nr
Match: gi|659097645|ref|XP_008449736.1| (PREDICTED: pre-mRNA-splicing factor CWC22 homolog isoform X2 [Cucumis melo])

HSP 1 Score: 1555.0 bits (4025), Expect = 0.0e+00
Identity = 777/797 (97.49%), Postives = 784/797 (98.37%), Query Frame = 1

Query: 1   MDRKHSRESSDEDEVKGNRRTSRDNDRQDRDKRHQDKNLHREVERGDKKKSYESFDEGEQ 60
           MDRK+SRESSDEDE KGNRR SRDNDRQDRD+RHQDKNLHREVERGDKKKSYESFDEGEQ
Sbjct: 1   MDRKYSRESSDEDEAKGNRRASRDNDRQDRDRRHQDKNLHREVERGDKKKSYESFDEGEQ 60

Query: 61  RTERTKSRHDRDRRHHDEKRHSRGQERENRKESYQSSDEDERKNRRHDRRQRETENENKN 120
           RTER KSRHDRDRRHHDEKRHSRG ERENRKESYQSSDEDERKNRRHDRRQRE ENENKN
Sbjct: 61  RTERNKSRHDRDRRHHDEKRHSRGHERENRKESYQSSDEDERKNRRHDRRQREIENENKN 120

Query: 121 YDRRLDHDSKDERDKELLRKSDHRRNRTDSDRERRHSKHDYKHNNFQDQRDKEASGRRED 180
            DRRLDHDSKDERDKELLRKSDHRRNR D+DRERRHSKHDYKHNN QDQRDKE SGRRED
Sbjct: 121 MDRRLDHDSKDERDKELLRKSDHRRNRADNDRERRHSKHDYKHNNSQDQRDKETSGRRED 180

Query: 181 RRNIGEKEKVGTGTIDHLQPHRQEKQTENNLNPDSSNLGKSGGVYIPPFKLARMMKEVQD 240
           RRNIGEKEKVGTGTIDH QPHRQEKQTENNLNPDSSNLGKSGGVYIPPFKLARMMKEVQD
Sbjct: 181 RRNIGEKEKVGTGTIDHRQPHRQEKQTENNLNPDSSNLGKSGGVYIPPFKLARMMKEVQD 240

Query: 241 KSSIEYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLFCRSCMKSQMAS 300
           KSSIEYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLFCRSCMKSQMAS
Sbjct: 241 KSSIEYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLFCRSCMKSQMAS 300

Query: 301 PGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQV 360
           PGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQV
Sbjct: 301 PGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQV 360

Query: 361 AHEIIALELLTVLLENPTDDSVEVAVGFVTECGSILKDLSPKGLHGIFERFRGILHEGEI 420
           AHEIIALELLTVLLENPTDDSVEVAVGFVTECGSILKDLSPKGLHGIFERFRGILHEGEI
Sbjct: 361 AHEIIALELLTVLLENPTDDSVEVAVGFVTECGSILKDLSPKGLHGIFERFRGILHEGEI 420

Query: 421 DKRVQFLIEGLFAIRKAKFEGYPAVRPELDLVEQEDQLTHEISLQEEIDPEITLDIFKSD 480
           DKRVQFLIEGLFAIRKAKFEGYPAVRPELDLVEQEDQLTHEISLQEEIDPEITLDIFKSD
Sbjct: 421 DKRVQFLIEGLFAIRKAKFEGYPAVRPELDLVEQEDQLTHEISLQEEIDPEITLDIFKSD 480

Query: 481 PNFLENEKRYEDLKKNILGEESEDEEDRSDAGSDESEDEDEEDESEEEDEEQMQINDETE 540
           PNFLENEKRYEDLKKNILGEESEDEEDRSDAGSD+ EDEDEEDESEEEDEEQMQINDETE
Sbjct: 481 PNFLENEKRYEDLKKNILGEESEDEEDRSDAGSDDEEDEDEEDESEEEDEEQMQINDETE 540

Query: 541 TNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEIELCVMLLECCSQERTYLRYYGL 600
           TNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEIELCVMLLECCSQERTYLRYYGL
Sbjct: 541 TNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEIELCVMLLECCSQERTYLRYYGL 600

Query: 601 LGQRFCKINKVYQENFDKCFVQQYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVLSYIR 660
           LGQRFCKINKVYQENF+KCFVQQYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVLSYIR
Sbjct: 601 LGQRFCKINKVYQENFEKCFVQQYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVLSYIR 660

Query: 661 LTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLTDPTMQDSFESIFPRDNPKNTRFSIN 720
           LTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLTDPTMQDSFESIFPRDNPKNTRFSIN
Sbjct: 661 LTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLTDPTMQDSFESIFPRDNPKNTRFSIN 720

Query: 721 FFTSIGLGGLTENLREYLKNMPRLIMQQQKPVSDSDEESQSSDSSDSDSSSSESKSDSED 780
           FFTSIGLGGLTENLREYLKNMPRLIMQQQKPVSDSDEESQSSDSSDSDSSSSES+SDSED
Sbjct: 721 FFTSIGLGGLTENLREYLKNMPRLIMQQQKPVSDSDEESQSSDSSDSDSSSSESESDSED 780

Query: 781 SEIEDRRREKKRRKTRR 798
            E +DRRREKKRRKTRR
Sbjct: 781 RESKDRRREKKRRKTRR 797

BLAST of Cucsa.065410 vs. NCBI nr
Match: gi|659097643|ref|XP_008449735.1| (PREDICTED: pre-mRNA-splicing factor CWC22 homolog isoform X1 [Cucumis melo])

HSP 1 Score: 1449.1 bits (3750), Expect = 0.0e+00
Identity = 744/847 (87.84%), Postives = 763/847 (90.08%), Query Frame = 1

Query: 1   MDRKHSRESSDEDEVKGNRRTSRDNDRQDRD----------------------------- 60
           MDRK+SRESSDEDE KGNRR SRDNDRQDRD                             
Sbjct: 1   MDRKYSRESSDEDEAKGNRRASRDNDRQDRDRRHQDKNLHREVERGDKKKSYESFDEGEQ 60

Query: 61  ------------KRHQDKNLH-REVERGDKKKSYESFDEGEQRTERTKSRH--------D 120
                       +RH D+  H R  ER ++K+SY+S DE E++  R   R         +
Sbjct: 61  RTERNKSRHDRDRRHHDEKRHSRGHERENRKESYQSSDEDERKNRRHDRRQRETENENKN 120

Query: 121 RDRRHHDEKRHSRGQERENRKESYQSSDEDERKNRRHDRRQRETENENKNYDRRLDHDSK 180
            DRR  ++ +  R +E  NRKESYQSSDEDERKNRRHDRRQRE ENENKN DRRLDHDSK
Sbjct: 121 MDRRLDNDSKDERDKELLNRKESYQSSDEDERKNRRHDRRQREIENENKNMDRRLDHDSK 180

Query: 181 DERDKELLRKSDHRRNRTDSDRERRHSKHDYKHNNFQDQRDKEASGRREDRRNIGEKEKV 240
           DERDKELLRKSDHRRNR D+DRERRHSKHDYKHNN QDQRDKE SGRREDRRNIGEKEKV
Sbjct: 181 DERDKELLRKSDHRRNRADNDRERRHSKHDYKHNNSQDQRDKETSGRREDRRNIGEKEKV 240

Query: 241 GTGTIDHLQPHRQEKQTENNLNPDSSNLGKSGGVYIPPFKLARMMKEVQDKSSIEYQRLT 300
           GTGTIDH QPHRQEKQTENNLNPDSSNLGKSGGVYIPPFKLARMMKEVQDKSSIEYQRLT
Sbjct: 241 GTGTIDHRQPHRQEKQTENNLNPDSSNLGKSGGVYIPPFKLARMMKEVQDKSSIEYQRLT 300

Query: 301 WDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLFCRSCMKSQMASPGFTDVFAAL 360
           WDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLFCRSCMKSQMASPGFTDVFAAL
Sbjct: 301 WDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLFCRSCMKSQMASPGFTDVFAAL 360

Query: 361 VAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQVAHEIIALELL 420
           VAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQVAHEIIALELL
Sbjct: 361 VAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQVAHEIIALELL 420

Query: 421 TVLLENPTDDSVEVAVGFVTECGSILKDLSPKGLHGIFERFRGILHEGEIDKRVQFLIEG 480
           TVLLENPTDDSVEVAVGFVTECGSILKDLSPKGLHGIFERFRGILHEGEIDKRVQFLIEG
Sbjct: 421 TVLLENPTDDSVEVAVGFVTECGSILKDLSPKGLHGIFERFRGILHEGEIDKRVQFLIEG 480

Query: 481 LFAIRKAKFEGYPAVRPELDLVEQEDQLTHEISLQEEIDPEITLDIFKSDPNFLENEKRY 540
           LFAIRKAKFEGYPAVRPELDLVEQEDQLTHEISLQEEIDPEITLDIFKSDPNFLENEKRY
Sbjct: 481 LFAIRKAKFEGYPAVRPELDLVEQEDQLTHEISLQEEIDPEITLDIFKSDPNFLENEKRY 540

Query: 541 EDLKKNILGEESEDEEDRSDAGSDESEDEDEEDESEEEDEEQMQINDETETNLVNLRRTI 600
           EDLKKNILGEESEDEEDRSDAGSD+ EDEDEEDESEEEDEEQMQINDETETNLVNLRRTI
Sbjct: 541 EDLKKNILGEESEDEEDRSDAGSDDEEDEDEEDESEEEDEEQMQINDETETNLVNLRRTI 600

Query: 601 YLTIMSSVDFEEAGHKLLKIKLEPGQEIELCVMLLECCSQERTYLRYYGLLGQRFCKINK 660
           YLTIMSSVDFEEAGHKLLKIKLEPGQEIELCVMLLECCSQERTYLRYYGLLGQRFCKINK
Sbjct: 601 YLTIMSSVDFEEAGHKLLKIKLEPGQEIELCVMLLECCSQERTYLRYYGLLGQRFCKINK 660

Query: 661 VYQENFDKCFVQQYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVLSYIRLTEEDTTSSS 720
           VYQENF+KCFVQQYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVLSYIRLTEEDTTSSS
Sbjct: 661 VYQENFEKCFVQQYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVLSYIRLTEEDTTSSS 720

Query: 721 RIFIKILFQELSEHLGIRLLNERLTDPTMQDSFESIFPRDNPKNTRFSINFFTSIGLGGL 780
           RIFIKILFQELSEHLGIRLLNERLTDPTMQDSFESIFPRDNPKNTRFSINFFTSIGLGGL
Sbjct: 721 RIFIKILFQELSEHLGIRLLNERLTDPTMQDSFESIFPRDNPKNTRFSINFFTSIGLGGL 780

Query: 781 TENLREYLKNMPRLIMQQQKPVSDSDEESQSSDSSDSDSSSSESKSDSEDSEIEDRRREK 798
           TENLREYLKNMPRLIMQQQKPVSDSDEESQSSDSSDSDSSSSES+SDSED E +DRRREK
Sbjct: 781 TENLREYLKNMPRLIMQQQKPVSDSDEESQSSDSSDSDSSSSESESDSEDRESKDRRREK 840

BLAST of Cucsa.065410 vs. NCBI nr
Match: gi|703075896|ref|XP_010090194.1| (Pre-mRNA-splicing factor CWC22-like protein [Morus notabilis])

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 593/837 (70.85%), Postives = 689/837 (82.32%), Query Frame = 1

Query: 2   DRKHSRESSDEDEVKGNRR------TSRDNDRQDRDKRHQ--------DKNLHREVE--- 61
           DR H R SS  D    +RR       S D+DR +R KRH+        DK+  RE +   
Sbjct: 20  DRHHHRSSSRRDSRSYDRRGRGDESDSSDSDRGNRRKRHEGRESRHSDDKDKERETDARD 79

Query: 62  RG--------DKKKSYESFDEGEQRTERTKSRHDRDRRHHDEKRHSRGQERENR--KESY 121
           RG        D++ S  S+DE +++ E+ +   +R+R    +  H + +  E+   +ES+
Sbjct: 80  RGRWRASGQEDRRPSQRSYDEDDEKVEKERGLRERERARQQDNGHKKRESEEDDMVRESH 139

Query: 122 QSSDEDERKNRR------------HDRRQRETENENKNYDRRLDHDSKDERDKELLRKSD 181
           QSS+ED++K +R            +D R +ETE   +N DR   H  +DE D+E  R +D
Sbjct: 140 QSSEEDDQKIKRDRREKEKTRRYDNDGRTQETEYGYRNRDRHSRHYLEDEDDREEQR-TD 199

Query: 182 HRRNRTDSDRERRHSKHDYKHNNFQDQRDKEASGRREDRRNIGEKEKVGTGTIDHLQPHR 241
            +R+R   +++RRH + D +  + ++ RDK+   +RED +++ ++       +++ +P  
Sbjct: 200 RKRDRKGDNKQRRHRREDER--DLRNHRDKDEKEQREDSQSLRQRSDGNGKLLENSKPEG 259

Query: 242 QEKQTENNLNPDSSNLGKSGGVYIPPFKLARMMKEVQDKSSIEYQRLTWDALRKSINGLV 301
           Q    ENNL+ D+SNLGKSGGVY+PPFKLARMMKEVQDKSS+EYQRLTWDALRKSINGLV
Sbjct: 260 QST-VENNLSADASNLGKSGGVYVPPFKLARMMKEVQDKSSVEYQRLTWDALRKSINGLV 319

Query: 302 NKVNATNIKNIIPELFAENLIRGRGLFCRSCMKSQMASPGFTDVFAALVAVVNTKFPEVG 361
           NKVNATNIKNIIPELF+ENLIRGRGLFCRSCMKSQMASPGFTDVFAALVAVVNTKFPEVG
Sbjct: 320 NKVNATNIKNIIPELFSENLIRGRGLFCRSCMKSQMASPGFTDVFAALVAVVNTKFPEVG 379

Query: 362 DLLLRRIVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQVAHEIIALELLTVLLENPTDDSV 421
           +L+LRRIVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQVAHEI+ALELLTVLLENPTDDSV
Sbjct: 380 ELILRRIVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQVAHEIVALELLTVLLENPTDDSV 439

Query: 422 EVAVGFVTECGSILKDLSPKGLHGIFERFRGILHEGEIDKRVQFLIEGLFAIRKAKFEGY 481
           EVAVGFVTECGSIL+DLSPKGLHGIFERFRGILHEGEIDKRVQFLIEGLFAIRKAKF+GY
Sbjct: 440 EVAVGFVTECGSILQDLSPKGLHGIFERFRGILHEGEIDKRVQFLIEGLFAIRKAKFQGY 499

Query: 482 PAVRPELDLVEQEDQLTHEISLQEEIDPEITLDIFKSDPNFLENEKRYEDLKKNILGEES 541
           PAVRPELDLVEQEDQLTHEISLQEEIDPEITLD+FK DPN+ ENEKRYE+L+KNILGEES
Sbjct: 500 PAVRPELDLVEQEDQLTHEISLQEEIDPEITLDLFKPDPNYSENEKRYEELRKNILGEES 559

Query: 542 EDEEDRSDAGSDESEDEDEEDESEEEDEEQMQINDETETNLVNLRRTIYLTIMSSVDFEE 601
           EDEE  SD  SD +ED+D++D+SEEE++E+MQI DETETNLVNLRRTIYLTIMSSVDFEE
Sbjct: 560 EDEEG-SDTSSD-AEDDDDDDDSEEEEDEEMQIKDETETNLVNLRRTIYLTIMSSVDFEE 619

Query: 602 AGHKLLKIKLEPGQEIELCVMLLECCSQERTYLRYYGLLGQRFCKINKVYQENFDKCFVQ 661
           AGHKLLKIKLEPGQE+ELC+MLLECCSQERTYLRYYGLLGQRFC INKV+QENF+KCFVQ
Sbjct: 620 AGHKLLKIKLEPGQEMELCIMLLECCSQERTYLRYYGLLGQRFCMINKVHQENFEKCFVQ 679

Query: 662 QYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVLSYIRLTEEDTTSSSRIFIKILFQELS 721
           QYSMIHRLETNKLRNVAKFFAHLLGTDALPWH L+YIRLTEEDTTSSSRIFIKILFQELS
Sbjct: 680 QYSMIHRLETNKLRNVAKFFAHLLGTDALPWHCLAYIRLTEEDTTSSSRIFIKILFQELS 739

Query: 722 EHLGIRLLNERLTDPTMQDSFESIFPRDNPKNTRFSINFFTSIGLGGLTENLREYLKNMP 781
           EHLG+RLLNERL DP+MQDSFESIFP+DNPKNTRF+INFFTSIGLGG+TENLREYLKNMP
Sbjct: 740 EHLGLRLLNERLNDPSMQDSFESIFPKDNPKNTRFAINFFTSIGLGGITENLREYLKNMP 799

Query: 782 RLIMQQQKPV--SDSDEESQSSDSSDSDSSSSESKSDSEDSEIEDRRREKKRRKTRR 798
            LIMQQQK V  S+SD+E+ SS SSDSDS+SSES+  S   E + ++ EK+R++ RR
Sbjct: 800 NLIMQQQKHVSESESDDEAGSSGSSDSDSASSESEESSSSDESKSKKDEKRRKRRRR 850

BLAST of Cucsa.065410 vs. NCBI nr
Match: gi|356524175|ref|XP_003530707.1| (PREDICTED: pre-mRNA-splicing factor CWC22 homolog [Glycine max])

HSP 1 Score: 1126.3 bits (2912), Expect = 0.0e+00
Identity = 587/805 (72.92%), Postives = 658/805 (81.74%), Query Frame = 1

Query: 2   DRKHSRESSDEDEVKGNRRTSRDNDRQDRDKRHQDKNLHREVERGDK--KKSYESFDEGE 61
           +RK S  S +E ++    R   +     R +R  D    R+ E  D+  ++S+   D+ E
Sbjct: 75  ERKESESSEEEGQIVSKERIREEKKEDARLQRRHDGRGGRDNEGDDRDGRESHRGNDQDE 134

Query: 62  QR--TERTKSRH-------DRDRRHHDEKRHSRGQERENRKESYQSSDEDERKNRRHDRR 121
           +R  TE  +  H       D DR+  +++R     E E+ +E  + S+ D   NR  DRR
Sbjct: 135 KRGETESHRLHHGDRWRGGDEDRQKINDRRGRHDSEEEDYRELARKSERDRDNNRDRDRR 194

Query: 122 QRETENENKNYDRRLDHDSKDERDKELLRKSDHRRNRT-DSDRERRHSKHDYKHNNFQDQ 181
           QR  +  +   DRR+ H+S++E  +EL RK D  R+R  D DR RR  K      N    
Sbjct: 195 QRVEKGGDNERDRRMRHNSEEEDARELARKLDRDRDRDRDRDRRRRVEKDGGSERN---H 254

Query: 182 RDKEASGRREDRRNIGEKEKVGTGTIDHLQPHRQEKQTENNLNPDSSNLGKSGGVYIPPF 241
           R+K+ S R+E+ R   +KE      ++  +P RQ      NLN D+SNLGKSGGVYIPPF
Sbjct: 255 REKDVSKRQEESR---KKEDGNGRMVEDEKPQRQATIPGGNLNGDASNLGKSGGVYIPPF 314

Query: 242 KLARMMKEVQDKSSIEYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLF 301
           K+A MMKEVQDKSS+EYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLF
Sbjct: 315 KMAMMMKEVQDKSSVEYQRLTWDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLF 374

Query: 302 CRSCMKSQMASPGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAV 361
           CRSCMKSQMASPGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAV
Sbjct: 375 CRSCMKSQMASPGFTDVFAALVAVVNTKFPEVGDLLLRRIVLQLKRAYKRNDKPQLLAAV 434

Query: 362 KFIAHLVNQQVAHEIIALELLTVLLENPTDDSVEVAVGFVTECGSILKDLSPKGLHGIFE 421
           KFIAHLVNQQ AHEIIALELLTVLLE PTDDSVEVAVGFVTECGSIL+DLSPKGLHGIFE
Sbjct: 435 KFIAHLVNQQAAHEIIALELLTVLLEKPTDDSVEVAVGFVTECGSILQDLSPKGLHGIFE 494

Query: 422 RFRGILHEGEIDKRVQFLIEGLFAIRKAKFEGYPAVRPELDLVEQEDQLTHEISLQEEID 481
           RFRGILHEGEIDKRVQFLIEGLFAIRKAKF+GYPAVRPELDLVEQEDQ+THE+SL EEID
Sbjct: 495 RFRGILHEGEIDKRVQFLIEGLFAIRKAKFQGYPAVRPELDLVEQEDQITHEVSLDEEID 554

Query: 482 PEITLDIFKSDPNFLENEKRYEDLKKNILGEESEDEEDRSDAGSDESEDEDEEDESEEED 541
           PEI+LDIFK DPNFLENEKRYE+LKK++LGEESED+E+  D+ SD  +D+DE+DES+EE+
Sbjct: 555 PEISLDIFKPDPNFLENEKRYEELKKSMLGEESEDDEEGLDSESD--DDDDEDDESDEEE 614

Query: 542 EEQMQINDETETNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEIELCVMLLECCS 601
           EEQMQI DETETNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQE+ELC+MLLECCS
Sbjct: 615 EEQMQIKDETETNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEMELCIMLLECCS 674

Query: 602 QERTYLRYYGLLGQRFCKINKVYQENFDKCFVQQYSMIHRLETNKLRNVAKFFAHLLGTD 661
           QERTYLRYYGLLGQRFC INKV+QENF+KCFVQQYSMIHRLETNKLRNV KFFAHLLGTD
Sbjct: 675 QERTYLRYYGLLGQRFCMINKVHQENFEKCFVQQYSMIHRLETNKLRNVGKFFAHLLGTD 734

Query: 662 ALPWHVLSYIRLTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLTDPTMQDSFESIFPR 721
           ALPWHVLSYIRLTEEDTTSSSRIFIKILFQELSEHLGIRLLNERL DPTMQ+SFESIFP+
Sbjct: 735 ALPWHVLSYIRLTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLNDPTMQESFESIFPK 794

Query: 722 DNPKNTRFSINFFTSIGLGGLTENLREYLKNMPRLIMQQQKPVSDSDEESQSSDSSDSDS 781
           DNPKNTRF INFFTSIGLGGLTENLREYLKNMPRLIMQQQK VS+S+ + +S  S  SDS
Sbjct: 795 DNPKNTRFCINFFTSIGLGGLTENLREYLKNMPRLIMQQQKQVSESESDDESGSSDSSDS 854

Query: 782 SSSESKSDSEDSEIEDRRREKKRRK 795
            ++ S SDS  S+  DRRR K+RRK
Sbjct: 855 GTASSDSDSASSDESDRRRRKRRRK 871