Cucsa.031700 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.031700
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	TPX2 (targeting protein for Xklp2) protein family
Location	scaffold00429 : 895552 .. 896308 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATAGAAGAGGAAGAGGTTCGTTTGATGAGAAAGGAAATGATACCAAGAGCTCAACTCATGCCCTACTTTGATAGACCCTACTTTCCACAAAGGTAGTGCACAAAATAGCTTTTATAAATTATATAATATTTTAAAATAACGTTTAGACGGCCATTCTAGACTGCATCTATCTTAATTATAAGAATATCCATCCTTAGTCATTACTTAACAAACCCTTTTAAGTATATACAAAAAAAAAaCTCTTTTATATTTACTCTTTTCCTTTGTGATTGAAATATATACATGGAGATATGAGAAATGTAGCATGAGCTTCATCCGAAGTATTGTATCTACATTTTTTTAAAACAATGACTTTTATAGGGGGCAAGAATGAGATTGAGTTTTGCACCTACTGATCTCTTTCTCAGTTGTATCTCTTTATGTTGTGTATGATTGTTAGAAAAAAGTGGTAGATCAAAAAGGTACTTTTAATAATTCCATAAGTGTTTACTGATTTATTACATGGTTCAAAAGTCATATTTGAAAGTAATACAAGTGCACACCCAAATGCTTGATTGTTATGAGGAAAAGTAAAACGTTTTTTAAAAGTATCAATGAAACTATATCTTGATGCCCTAAAGAAACTTTTCACTTTTAAAATGCTATTTTCTTGTAAAAAAAAGAAaGGATTGTGATTATTTGTAATGCAGATCGAATCGACCTTTGACGATACCGAGAGAGCCGAGTTTCTTGATGAACAAAGAGCAATGGAGTT

mRNA sequence

ATGATAGAAGAGGAAGAGGTTCGTTTGATGAGAAAGGAAATGATACCAAGAGCTCAACTCATGCCCTACTTTGATAGACCCTACTTTCCACAAAGATCGAATCGACCTTTGACGATACCGAGAGAGCCGAGTTTCTTGATGAACAAAGAGCAATGGAGTT

Coding sequence (CDS)

ATGATAGAAGAGGAAGAGGTTCGTTTGATGAGAAAGGAAATGATACCAAGAGCTCAACTCATGCCCTACTTTGATAGACCCTACTTTCCACAAAGATCGAATCGACCTTTGACGATACCGAGAGAGCCGAGTTTCTTGATGAACAAAGAGCAATGGAGTT

Protein sequence

MIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSFLMNKEQWSX

Homology

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BLAST of Cucsa.031700 vs. TrEMBL
Match: A0A0A0LCD4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G258180 PE=4 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 7.4e-23
Identity = 53/53 (100.00%), Postives = 53/53 (100.00%), Query Frame = 1


Query: 1   MIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSFLMNKEQWS 54
           MIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSFLMNKEQWS
Sbjct: 84  MIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSFLMNKEQWS 136

[Close]

BLAST of Cucsa.031700 vs. TrEMBL
Match: B9SR84_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0112060 PE=4 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 6.1e-17
Identity = 43/54 (79.63%), Postives = 51/54 (94.44%), Query Frame = 1


Query: 1   MIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSF-LMNKEQWS 54
           +IEEEEVR++RKEMIPRAQLMP+FDRP+FPQRSNRPLTIPREPSF ++N + WS
Sbjct: 99  IIEEEEVRMLRKEMIPRAQLMPFFDRPFFPQRSNRPLTIPREPSFHMLNSKCWS 152

[Close]

BLAST of Cucsa.031700 vs. TrEMBL
Match: A5X7Z1_9ROSI (Microtubule-associated protein OS=Populus tremula x Populus tremuloides GN=MAP20 PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 8.0e-17
Identity = 42/54 (77.78%), Postives = 51/54 (94.44%), Query Frame = 1


Query: 1   MIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSF-LMNKEQWS 54
           +IEEEEVR+MRKEM+PRAQLMPYFDRP+FPQRS+RPLT+PREPSF ++N + WS
Sbjct: 98  IIEEEEVRMMRKEMVPRAQLMPYFDRPFFPQRSSRPLTVPREPSFHMVNSKCWS 151

[Close]

BLAST of Cucsa.031700 vs. TrEMBL
Match: A9P8J7_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s01510g PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.8e-16
Identity = 42/54 (77.78%), Postives = 50/54 (92.59%), Query Frame = 1


Query: 1   MIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSF-LMNKEQWS 54
           +IEEEEVR MRKEM+PRAQLMPYFDRP+FPQRS+RPLT+PREPSF ++N + WS
Sbjct: 98  IIEEEEVRTMRKEMVPRAQLMPYFDRPFFPQRSSRPLTVPREPSFHMVNSKCWS 151

[Close]

BLAST of Cucsa.031700 vs. TrEMBL
Match: A0A067KHS4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_10523 PE=4 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 2.3e-16
Identity = 43/54 (79.63%), Postives = 50/54 (92.59%), Query Frame = 1


Query: 1  MIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSF-LMNKEQWS 54
          +IEEEEVR +RKEMIPRAQLMP+FDRP+FPQRSNRPLTIPREPSF ++N + WS
Sbjct: 14 IIEEEEVRSLRKEMIPRAQLMPFFDRPFFPQRSNRPLTIPREPSFRMVNSKCWS 67

[Close]

BLAST of Cucsa.031700 vs. TAIR10
Match: AT5G37478.1 (AT5G37478.1 TPX2 (targeting protein for Xklp2) protein family)

HSP 1 Score: 82.8 bits (203), Expect = 7.1e-17
Identity = 34/45 (75.56%), Postives = 44/45 (97.78%), Query Frame = 1


Query: 1   MIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSF 46
           MIEEEE+R++RKEM+P+AQLMP+FDRP+ PQRS+RPLT+P+EPSF
Sbjct: 108 MIEEEEIRMLRKEMVPKAQLMPFFDRPFLPQRSSRPLTMPKEPSF 152

[Close]

BLAST of Cucsa.031700 vs. TAIR10
Match: AT3G01015.1 (AT3G01015.1 TPX2 (targeting protein for Xklp2) protein family)

HSP 1 Score: 68.2 bits (165), Expect = 1.8e-12
Identity = 27/45 (60.00%), Postives = 38/45 (84.44%), Query Frame = 1


Query: 1   MIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSF 46
           + EEEE+R +RKE++P+AQ MPYFDRP+ P+RSN+  T+PR+P F
Sbjct: 412 LAEEEEIRRLRKELVPKAQPMPYFDRPFIPKRSNKHPTVPRDPKF 456

[Close]

BLAST of Cucsa.031700 vs. TAIR10
Match: AT5G15510.2 (AT5G15510.2 TPX2 (targeting protein for Xklp2) protein family)

HSP 1 Score: 52.0 bits (123), Expect = 1.3e-07
Identity = 20/32 (62.50%), Postives = 28/32 (87.50%), Query Frame = 1


Query: 1   MIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQR 33
           + EEEE+R +RKE++P+AQ MPYFDRP+ P+R
Sbjct: 422 LAEEEEIRRLRKELVPKAQPMPYFDRPFIPRR 453

[Close]

BLAST of Cucsa.031700 vs. NCBI nr
Match: gi|449455828|ref|XP_004145652.1| (PREDICTED: protein TPX2-like [Cucumis sativus])

HSP 1 Score: 113.6 bits (283), Expect = 1.1e-22
Identity = 53/53 (100.00%), Postives = 53/53 (100.00%), Query Frame = 1


Query: 1   MIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSFLMNKEQWS 54
           MIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSFLMNKEQWS
Sbjct: 84  MIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSFLMNKEQWS 136

[Close]

BLAST of Cucsa.031700 vs. NCBI nr
Match: gi|255575198|ref|XP_002528503.1| (PREDICTED: protein TPX2 isoform X1 [Ricinus communis])

HSP 1 Score: 94.0 bits (232), Expect = 8.7e-17
Identity = 43/54 (79.63%), Postives = 51/54 (94.44%), Query Frame = 1


Query: 1   MIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSF-LMNKEQWS 54
           +IEEEEVR++RKEMIPRAQLMP+FDRP+FPQRSNRPLTIPREPSF ++N + WS
Sbjct: 99  IIEEEEVRMLRKEMIPRAQLMPFFDRPFFPQRSNRPLTIPREPSFHMLNSKCWS 152

[Close]

BLAST of Cucsa.031700 vs. NCBI nr
Match: gi|1000947914|ref|XP_015580432.1| (PREDICTED: protein TPX2 isoform X2 [Ricinus communis])

HSP 1 Score: 94.0 bits (232), Expect = 8.7e-17
Identity = 43/54 (79.63%), Postives = 51/54 (94.44%), Query Frame = 1


Query: 1   MIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSF-LMNKEQWS 54
           +IEEEEVR++RKEMIPRAQLMP+FDRP+FPQRSNRPLTIPREPSF ++N + WS
Sbjct: 95  IIEEEEVRMLRKEMIPRAQLMPFFDRPFFPQRSNRPLTIPREPSFHMLNSKCWS 148

[Close]

BLAST of Cucsa.031700 vs. NCBI nr
Match: gi|110277459|gb|ABG57264.1| (microtubule-associated protein [Populus tremula x Populus tremuloides])

HSP 1 Score: 93.6 bits (231), Expect = 1.1e-16
Identity = 42/54 (77.78%), Postives = 51/54 (94.44%), Query Frame = 1


Query: 1   MIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSF-LMNKEQWS 54
           +IEEEEVR+MRKEM+PRAQLMPYFDRP+FPQRS+RPLT+PREPSF ++N + WS
Sbjct: 98  IIEEEEVRMMRKEMVPRAQLMPYFDRPFFPQRSSRPLTVPREPSFHMVNSKCWS 151

[Close]

BLAST of Cucsa.031700 vs. NCBI nr
Match: gi|743888273|ref|XP_011038399.1| (PREDICTED: protein TPX2-like isoform X1 [Populus euphratica])

HSP 1 Score: 93.6 bits (231), Expect = 1.1e-16
Identity = 42/54 (77.78%), Postives = 51/54 (94.44%), Query Frame = 1


Query: 1   MIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSF-LMNKEQWS 54
           +IEEEEVR+MRKEM+PRAQLMPYFDRP+FPQRS+RPLT+PREPSF ++N + WS
Sbjct: 98  IIEEEEVRMMRKEMVPRAQLMPYFDRPFFPQRSSRPLTVPREPSFHMVNSKCWS 151

The following BLAST results are available for this feature:

BLAST of Cucsa.031700 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LCD4_CUCSA	7.4e-23	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G258180 PE=4 SV=1	[more]
B9SR84_RICCO	6.1e-17	79.63	Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0112060 PE=4 SV=1	[more]
A5X7Z1_9ROSI	8.0e-17	77.78	Microtubule-associated protein OS=Populus tremula x Populus tremuloides GN=MAP20...	[more]
A9P8J7_POPTR	1.8e-16	77.78	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s01510g PE=2 SV=1	[more]
A0A067KHS4_JATCU	2.3e-16	79.63	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_10523 PE=4 SV=1	[more]

BLAST of Cucsa.031700 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G37478.1	7.1e-17	75.56	TPX2 (targeting protein for Xklp2) protein family	[more]
AT3G01015.1	1.8e-12	60.00	TPX2 (targeting protein for Xklp2) protein family	[more]
AT5G15510.2	1.3e-07	62.50	TPX2 (targeting protein for Xklp2) protein family	[more]

BLAST of Cucsa.031700 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449455828\|ref\|XP_004145652.1\|	1.1e-22	100.00	PREDICTED: protein TPX2-like [Cucumis sativus]	[more]
gi\|255575198\|ref\|XP_002528503.1\|	8.7e-17	79.63	PREDICTED: protein TPX2 isoform X1 [Ricinus communis]	[more]
gi\|1000947914\|ref\|XP_015580432.1\|	8.7e-17	79.63	PREDICTED: protein TPX2 isoform X2 [Ricinus communis]	[more]
gi\|110277459\|gb\|ABG57264.1\|	1.1e-16	77.78	microtubule-associated protein [Populus tremula x Populus tremuloides]	[more]
gi\|743888273\|ref\|XP_011038399.1\|	1.1e-16	77.78	PREDICTED: protein TPX2-like isoform X1 [Populus euphratica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR009675	TPX2_fam

Vocabulary: **Cellular Component**
Term	Definition
GO:0005819	spindle
GO:0005874	microtubule

Vocabulary: **Biological Process**
Term	Definition
GO:0032147	activation of protein kinase activity
GO:0060236	regulation of mitotic spindle organization

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032147	activation of protein kinase activity
biological_process	GO:0007067	mitotic nuclear division
biological_process	GO:0060236	regulation of mitotic spindle organization
cellular_component	GO:0005874	microtubule
cellular_component	GO:0005819	spindle
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.031700.1	Cucsa.031700.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009675	TPX2	PANTHER	PTHR14326	TARGETING PROTEIN FOR XKLP2	coord: 1..54 score: 9.9

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.031700	Csa3G258180	Cucumber (Chinese Long) v2	cgycuB003
Cucsa.031700	CSPI03G20360	Wild cucumber (PI 183967)	cgycpiB003
Cucsa.031700	CmaCh01G003980	Cucurbita maxima (Rimu)	cgycmaB0011
Cucsa.031700	CmoCh01G004220	Cucurbita moschata (Rifu)	cgycmoB0010
Cucsa.031700	Lsi05G004080	Bottle gourd (USVL1VR-Ls)	cgylsiB004
Cucsa.031700	Cp4.1LG02g17130	Cucurbita pepo (Zucchini)	cgycpeB0008
Cucsa.031700	MELO3C032079.2	Melon (DHL92) v3.6.1	cgymedB004
Cucsa.031700	Bhi01G001435	Wax gourd	cgywgoB005
Cucsa.031700	Carg07491	Silver-seed gourd	carcgyB0854

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.031700	Cucurbita pepo (Zucchini)	cgycpeB0009
Cucsa.031700	Silver-seed gourd	carcgyB0552
Cucsa.031700	Cucumber (Chinese Long) v3	cgycucB006
Cucsa.031700	Watermelon (97103) v2	cgywmbB004
Cucsa.031700	Cucurbita maxima (Rimu)	cgycmaB0005
Cucsa.031700	Cucurbita moschata (Rifu)	cgycmoB0004
Cucsa.031700	Melon (DHL92) v3.5.1	cgymeB003
Cucsa.031700	Watermelon (Charleston Gray)	cgywcgB004
Cucsa.031700	Watermelon (97103) v1	cgywmB003