Cucsa.031380 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.031380
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase
Location	scaffold00429 : 474036 .. 474828 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTCTATCACGTCTTGCTCCCTTTGAGAGAGGATTGTATGAAGATTATGTGGCGAACTTTTGGTGTACCACGTCTGTCCTTATTAAGTGGAAGAGATTGTTTTCAGTGAAATTACTGAAAATTTTGAGCTTCACTGCTACACTATCAACTTGTTTACCTTCCATGATTCAACAGATTCGTGTCCCAAGTAACCAGGGGTTTCTATATGGCCTTTTATGTAGTTCTTTCTCATTCTACCTGTTCTCGTTTCAAGGTACTTGCTCTATCTCTCTCCCTTTTTTTCTCTCTCTCTCCGCGAGCTAGGATAATATTTTTATGAAGATAGCCTAATTAACCGCCTCTGATTAACATTGCAGTGCATGAGAAGTCTATTCTTCTTCCTCTTCTTCCTGCAAGCATGTTGGCCCTGGATGAGCCTTCATTATTTATTCATTTTCTACACTATGCATTGCTTTCAATATTTCCTCTTGTAGTTCGAGACAAACTGGTTCAAGCATATCTTGCCATTTATGCTCTTACCTTTCTTATTATCAATGCATTAAATAAAGGAAAGCAAAAAGGGGGAGGATTTCATTCTGGGGGGGTTTTATTTGGATGTTTCCTGTTTTGTTCACTTGTTCTCCACGTTGTGTACTTGGTGGTGCGCCCTCCTGAGAGGTATCCCTTTCTCTTTGAGGCAGTAATTATGCTTCTTTGCTTTTCTCAATTCATCTTCCTTGTGATCTTCTCCAATGTAAAGCAATGGACGCTGTCTAAAGCTGTTCCACAGATGCACAAGCAAAAGCTTAATTAG

mRNA sequence

GTTCTATCACGTCTTGCTCCCTTTGAGAGAGGATTGTATGAAGATTATGTGGCGAACTTTTGGTGTACCACGTCTGTCCTTATTAAGTGGAAGAGATTGTTTTCAGTGAAATTACTGAAAATTTTGAGCTTCACTGCTACACTATCAACTTGTTTACCTTCCATGATTCAACAGATTCGTGTCCCAAGTAACCAGGGGTTTCTATATGGCCTTTTATGTAGTTCTTTCTCATTCTACCTGTTCTCGTTTCAAGTGCATGAGAAGTCTATTCTTCTTCCTCTTCTTCCTGCAAGCATGTTGGCCCTGGATGAGCCTTCATTATTTATTCATTTTCTACACTATGCATTGCTTTCAATATTTCCTCTTGTAGTTCGAGACAAACTGGTTCAAGCATATCTTGCCATTTATGCTCTTACCTTTCTTATTATCAATGCATTAAATAAAGGAAAGCAAAAAGGGGGAGGATTTCATTCTGGGGGGGTTTTATTTGGATGTTTCCTGTTTTGTTCACTTGTTCTCCACGTTGTGTACTTGGTGGTGCGCCCTCCTGAGAGGTATCCCTTTCTCTTTGAGGCAGTAATTATGCTTCTTTGCTTTTCTCAATTCATCTTCCTTGTGATCTTCTCCAATGTAAAGCAATGGACGCTGTCTAAAGCTGTTCCACAGATGCACAAGCAAAAGCTTAATTAG

Coding sequence (CDS)

GTTCTATCACGTCTTGCTCCCTTTGAGAGAGGATTGTATGAAGATTATGTGGCGAACTTTTGGTGTACCACGTCTGTCCTTATTAAGTGGAAGAGATTGTTTTCAGTGAAATTACTGAAAATTTTGAGCTTCACTGCTACACTATCAACTTGTTTACCTTCCATGATTCAACAGATTCGTGTCCCAAGTAACCAGGGGTTTCTATATGGCCTTTTATGTAGTTCTTTCTCATTCTACCTGTTCTCGTTTCAAGTGCATGAGAAGTCTATTCTTCTTCCTCTTCTTCCTGCAAGCATGTTGGCCCTGGATGAGCCTTCATTATTTATTCATTTTCTACACTATGCATTGCTTTCAATATTTCCTCTTGTAGTTCGAGACAAACTGGTTCAAGCATATCTTGCCATTTATGCTCTTACCTTTCTTATTATCAATGCATTAAATAAAGGAAAGCAAAAAGGGGGAGGATTTCATTCTGGGGGGGTTTTATTTGGATGTTTCCTGTTTTGTTCACTTGTTCTCCACGTTGTGTACTTGGTGGTGCGCCCTCCTGAGAGGTATCCCTTTCTCTTTGAGGCAGTAATTATGCTTCTTTGCTTTTCTCAATTCATCTTCCTTGTGATCTTCTCCAATGTAAAGCAATGGACGCTGTCTAAAGCTGTTCCACAGATGCACAAGCAAAAGCTTAATTAG

Protein sequence

VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFSVKLLKILSFTATLSTCLPSMIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLHVVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN*

Homology

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BLAST of Cucsa.031380 vs. Swiss-Prot
Match: ALG6_ARATH (Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Arabidopsis thaliana GN=At5g38460 PE=2 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 1.9e-65
Identity = 132/236 (55.93%), Postives = 164/236 (69.49%), Query Frame = 1


Query: 1   VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFSVKLLKILSFTATLSTCLPSMIQQIR 60
           VLSRLAPFERG+YEDYVANFWCTTS+LIKWK LF+ + LK +S  AT+   LPSM+QQI 
Sbjct: 295 VLSRLAPFERGIYEDYVANFWCTTSILIKWKNLFTTQSLKSISLAATILASLPSMVQQIL 354

Query: 61  VPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLSIF 120
            PSN+GFLYGLL SS +FYLFSFQVHEKSIL+P L A++LAL  P  F H  +YAL S+F
Sbjct: 355 SPSNEGFLYGLLNSSMAFYLFSFQVHEKSILMPFLSATLLALKLPDHFSHLTYYALFSMF 414

Query: 121 PLVVRDKLVQAYLAIYALTFLII------NALNKGKQKGGGFHS--GGV-LFGCFLFCSL 180
           PL+ RDKL+  YL +  L  +I       +A+ K       F +  G V L     F S+
Sbjct: 415 PLLCRDKLLIPYLTLSFLFTVIYHSPGNHHAIQKTDVSFFSFKNFPGYVFLLRTHFFISV 474

Query: 181 VLHVVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQK 228
           VLHV+YL ++PP++YPFLFEA+IM+LCFS FI    ++N  QWTLS       K+K
Sbjct: 475 VLHVLYLTIKPPQKYPFLFEALIMILCFSYFIMFAFYTNYTQWTLSSHFGSSDKEK 530

[Close]

BLAST of Cucsa.031380 vs. Swiss-Prot
Match: ALG6_DICDI (Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Dictyostelium discoideum GN=alg6 PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 7.2e-28
Identity = 88/236 (37.29%), Postives = 124/236 (52.54%), Query Frame = 1


Query: 1   VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFSVKLLKILSFTATLSTCLPSMIQQIR 60
           VL RL PF RGLYED VANFWC  S++I  K LF+   L  +    TL T LP +    R
Sbjct: 274 VLFRLFPFARGLYEDKVANFWCFISIIINVKNLFTTDQLIKICLILTLVTMLPLVYGIKR 333

Query: 61  VPSNQG-FLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLSI 120
           +P N+  F++ L+ SSFSF+LFSFQVHEK+ILL  LP S+L L  P++   F+  +  S+
Sbjct: 334 IPKNKFVFIHSLINSSFSFFLFSFQVHEKTILLVSLPISLLILHHPNMVWWFILISTFSM 393

Query: 121 FPLVVRDKLVQAYLAIYALTFL--------IINALNKGKQKGG----------GFHSGGV 180
           FPL+ +D LV  Y AI  L  +        I  + N+ K +             F+S  +
Sbjct: 394 FPLLFKDGLVIPYFAIMILYIVIGYQFKNSITRSNNQFKHQNSQENLLASSDKSFYSIYL 453

Query: 181 LFGCFL--FCSLVLHVVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWT 216
            +  +L     +V H++Y     P   P L+  ++    F  FI L  F  +K+ T
Sbjct: 454 NYWFYLNIIGMVVCHLLYQFAPHPPHLPSLWLLLVCNFSFIHFI-LTFFYFIKEMT 508

[Close]

BLAST of Cucsa.031380 vs. Swiss-Prot
Match: ALG6_PONAB (Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Pongo abelii GN=ALG6 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.6e-27
Identity = 74/233 (31.76%), Postives = 124/233 (53.22%), Query Frame = 1


Query: 1   VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFSVKLLKILSFTATLSTCLPSMIQQIR 60
           VL RL P +RGL+ED VAN WC+ +V +K K +    +  I+SF  T  + LP+ I+ I 
Sbjct: 259 VLRRLFPVDRGLFEDKVANIWCSFNVFLKIKDILPRHIQLIMSFCFTFLSLLPACIKLIL 318

Query: 61  VPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLSIF 120
            PS++GF + L+  + SF+LFSFQVHEKSILL  LP  ++  + P +   FL  +  S+ 
Sbjct: 319 QPSSKGFKFTLVSCALSFFLFSFQVHEKSILLVSLPVCLVLSEIPFMSTWFLLVSTFSML 378

Query: 121 PLVVRDKLV-------QAYLAIYALTFLIINALNKGKQKGGGFH-SGGVLFGCFLFCSLV 180
           PL+++D+L+        A+      +F I    ++ + +   F  S      CF F S +
Sbjct: 379 PLLLKDELLMPSVVTTMAFFIACVTSFSIFEKTSEEELQLKSFSISVRKYLPCFTFLSRI 438

Query: 181 LHVVYLV--------------VRPPERYPFLFEAVIMLLCFSQFIFLVIFSNV 212
           +  ++L+              + PP++ P LF  ++  +    F+F +++ N+
Sbjct: 439 IQYLFLISVITMVLLTLMTVTLGPPQKLPDLFSVLVCFVSCLNFLFFLVYFNI 491

[Close]

BLAST of Cucsa.031380 vs. Swiss-Prot
Match: ALG6_HUMAN (Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Homo sapiens GN=ALG6 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.6e-27
Identity = 74/233 (31.76%), Postives = 124/233 (53.22%), Query Frame = 1


Query: 1   VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFSVKLLKILSFTATLSTCLPSMIQQIR 60
           VL RL P +RGL+ED VAN WC+ +V +K K +    +  I+SF  T  + LP+ I+ I 
Sbjct: 259 VLRRLFPVDRGLFEDKVANIWCSFNVFLKIKDILPRHIQLIMSFCFTFLSLLPACIKLIL 318

Query: 61  VPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLSIF 120
            PS++GF + L+  + SF+LFSFQVHEKSILL  LP  ++  + P +   FL  +  S+ 
Sbjct: 319 QPSSKGFKFTLVSCALSFFLFSFQVHEKSILLVSLPVCLVLSEIPFMSTWFLLVSTFSML 378

Query: 121 PLVVRDKLV-------QAYLAIYALTFLIINALNKGKQKGGGFH-SGGVLFGCFLFCSLV 180
           PL+++D+L+        A+      +F I    ++ + +   F  S      CF F S +
Sbjct: 379 PLLLKDELLMPSVVTTMAFFIACVTSFSIFEKTSEEELQLKSFSISVRKYLPCFTFLSRI 438

Query: 181 LHVVYLV--------------VRPPERYPFLFEAVIMLLCFSQFIFLVIFSNV 212
           +  ++L+              + PP++ P LF  ++  +    F+F +++ N+
Sbjct: 439 IQYLFLISVITMVLLTLMTVTLDPPQKLPDLFSVLVCFVSCLNFLFFLVYFNI 491

[Close]

BLAST of Cucsa.031380 vs. Swiss-Prot
Match: ALG6_CHICK (Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Gallus gallus GN=ALG6 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.4e-26
Identity = 84/233 (36.05%), Postives = 118/233 (50.64%), Query Frame = 1


Query: 1   VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFSVKLLKILSFTATLSTCLPSMIQQIR 60
           VL RL P +RGL+ED VAN WC+ SVLIK K + S +    LSF  T  + LP+ I+   
Sbjct: 259 VLRRLFPIDRGLFEDKVANIWCSLSVLIKIKNVVSPQTQLKLSFAVTFLSLLPTCIKLTV 318

Query: 61  VPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLSIF 120
            PS +GF   L+  + SF+LFSFQVHEKSILL  +P  ++  + P +   FL  +  S+ 
Sbjct: 319 QPSLRGFKLTLVSCALSFFLFSFQVHEKSILLVSVPVCLIINEVPFMATWFLLVSTFSML 378

Query: 121 PLVVRDKLVQAY--------------LAIYALTFLIINALNKGKQKGGGFHSGGVLF--- 180
           PL+++D L+  Y               AI+  T      L    Q   G+ S   LF   
Sbjct: 379 PLLLKDGLLLPYAVTTLAFLSACVASFAIFEKTSAKDLQLKPFSQSLRGYVSWFKLFPKI 438

Query: 181 --GCFLFCSL---VLHVVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNV 212
               FL       VL V+   V PP+R+P LF   +  +    F+F +++ NV
Sbjct: 439 VRSLFLLSVTLMGVLSVMSAAVHPPQRFPDLFPVSVSSISCLHFLFFLVYFNV 491

[Close]

BLAST of Cucsa.031380 vs. TrEMBL
Match: A0A0A0L9U3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G271420 PE=4 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 5.3e-94
Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 1


Query: 55  MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY 114
           MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY
Sbjct: 1   MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY 60

Query: 115 ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLH 174
           ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLH
Sbjct: 61  ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLH 120

Query: 175 VVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN 230
           VVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN
Sbjct: 121 VVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN 175

[Close]

BLAST of Cucsa.031380 vs. TrEMBL
Match: W9R1J3_9ROSA (Putative dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Morus notabilis GN=L484_010418 PE=4 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 1.7e-76
Identity = 150/228 (65.79%), Postives = 173/228 (75.88%), Query Frame = 1


Query: 1   VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFSVKLLKILSFTATLSTCLPSMIQQIR 60
           VLSRLAPFERG+YEDYVANFWCTTSVLIKWKRLF+   LK+LSF AT+S CLPSM QQI 
Sbjct: 58  VLSRLAPFERGIYEDYVANFWCTTSVLIKWKRLFTTPSLKLLSFCATISACLPSMFQQIW 117

Query: 61  VPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLSIF 120
            PS QGFLYGLL S+FSFYLFSFQVHEKSILLPLLPAS+LA++EP +     HYAL S+F
Sbjct: 118 APSKQGFLYGLLNSAFSFYLFSFQVHEKSILLPLLPASLLAIEEPFVHSWMTHYALFSMF 177

Query: 121 PLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHS-GGVLFGCFLFCSLVLHVVYLV 180
           PLVVRDKLV  Y+A+  L FL   A     Q  G  +S    L   FLFC+L+LHVVY+ 
Sbjct: 178 PLVVRDKLVLPYIALSPLFFLFNRAGPTRAQATGEANSLRSFLTAFFLFCALILHVVYVT 237

Query: 181 VRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQK 228
           V PP RYPFLFEA+IMLLCFSQF+ L +++N KQW + K    + K K
Sbjct: 238 VNPPRRYPFLFEALIMLLCFSQFVSLTVYTNAKQWMMLKRPTLVDKDK 285

[Close]

BLAST of Cucsa.031380 vs. TrEMBL
Match: D7TT36_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0006g03000 PE=4 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 2.5e-75
Identity = 150/231 (64.94%), Postives = 176/231 (76.19%), Query Frame = 1


Query: 1   VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFSVKLLKILSFTATLSTCLPSMIQQIR 60
           VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLF++K +K+LS  AT+ TCLPSMIQQI 
Sbjct: 284 VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFTIKSMKLLSLWATILTCLPSMIQQIW 343

Query: 61  VPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLSIF 120
            PSN GFLYGLL SSFSFYLFSFQVHEKSILLPLLPA++LAL+EP  F    HYALLS+F
Sbjct: 344 SPSNLGFLYGLLNSSFSFYLFSFQVHEKSILLPLLPATLLALEEPFHFRWLTHYALLSMF 403

Query: 121 PLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVL----FGCFLFCSLVLHVV 180
           PL+ RDKL+  Y+A++AL +L+  A   G++      S   L        + CSL LH+V
Sbjct: 404 PLMCRDKLILPYVALFALFYLLYYAPG-GREDTRKMQSPSSLRSFMLSFLVLCSLFLHLV 463

Query: 181 YLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQK 228
           Y+ + PP++YPFLFEAVIMLLCFSQFI L I++N KQW L K    M K K
Sbjct: 464 YITLHPPDKYPFLFEAVIMLLCFSQFILLTIYTNAKQWMLLKHSTLMDKDK 513

[Close]

BLAST of Cucsa.031380 vs. TrEMBL
Match: A0A0K9RNA8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_053420 PE=4 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 2.1e-74
Identity = 143/229 (62.45%), Postives = 180/229 (78.60%), Query Frame = 1


Query: 1   VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFSVKLLKILSFTATLSTCLPSMIQQIR 60
           VL+RLAPFERG+YEDYVANFWCTTSVLIKWK+LF+   LK++S  ATLSTCLPSMIQQI+
Sbjct: 285 VLARLAPFERGIYEDYVANFWCTTSVLIKWKKLFNTSSLKLISLAATLSTCLPSMIQQIK 344

Query: 61  VPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLSIF 120
            PSN+GFLY LL SSFSFYLFSFQVHEKSILLPLLPAS+LAL+EP +F   +H+ L S+F
Sbjct: 345 DPSNKGFLYALLNSSFSFYLFSFQVHEKSILLPLLPASLLALEEPLVFRWLIHFGLFSMF 404

Query: 121 PLVVRDKLVQAYLAIYALTFLIINA-LNKGKQKGGGFHSGGVLFGCFL-FCSLVLHVVYL 180
           PL+ RD+LV AY+A+++L +L+  A   K   K   F +  +L    L FCS VLH+VYL
Sbjct: 405 PLLCRDQLVLAYIALFSLFYLLFYAPRKKDSGKIRSFFTLKLLITTLLVFCSFVLHIVYL 464

Query: 181 VVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQK 228
            + PP+RYPFLFEA+IML+ F+QF++L +++N KQW L K    + K+K
Sbjct: 465 AMNPPKRYPFLFEAIIMLVSFTQFVWLALYTNKKQWMLQKQSTCVEKEK 513

[Close]

BLAST of Cucsa.031380 vs. TrEMBL
Match: M5XQL1_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004184mg PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 2.7e-74
Identity = 148/230 (64.35%), Postives = 179/230 (77.83%), Query Frame = 1


Query: 1   VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFSVKLLKILSFTATLSTCLPSMIQQIR 60
           VLSRLAPFERG++EDYVANFWCTTSVL+KWKRLF  + LK+LSF AT+S+CLPSMIQQI 
Sbjct: 296 VLSRLAPFERGIFEDYVANFWCTTSVLVKWKRLFPTQTLKLLSFGATISSCLPSMIQQIW 355

Query: 61  VPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLSIF 120
            PS QGFLYGLL S+FSFYLFSFQVHEKSILLPLLPAS+LA +EP LF     YAL S+F
Sbjct: 356 SPSKQGFLYGLLNSAFSFYLFSFQVHEKSILLPLLPASLLATEEPFLFTWMTLYALFSMF 415

Query: 121 PLVVRDKLVQAYLAIYALTFLIINALNKGK---QKGGGFHSGGVLFGCFLFCSLVLHVVY 180
           PL+ RDKLV  YL++ AL F+++N L+ G+   +    F S   +   FL CS++LHVVY
Sbjct: 416 PLLHRDKLVLPYLSLSAL-FILLNHLSSGRRDTKDTSAFRS--FVTRVFLLCSVILHVVY 475

Query: 181 LVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQK 228
           L +  P RYPFLFEA+IMLLCFSQF+FL +++N KQW L K    + K+K
Sbjct: 476 LSMHAPARYPFLFEALIMLLCFSQFVFLTLYTNAKQWMLLKHSNLVDKEK 522

[Close]

BLAST of Cucsa.031380 vs. TAIR10
Match: AT5G38460.1 (AT5G38460.1 ALG6, ALG8 glycosyltransferase family)

HSP 1 Score: 250.4 bits (638), Expect = 1.1e-66
Identity = 132/236 (55.93%), Postives = 164/236 (69.49%), Query Frame = 1


Query: 1   VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFSVKLLKILSFTATLSTCLPSMIQQIR 60
           VLSRLAPFERG+YEDYVANFWCTTS+LIKWK LF+ + LK +S  AT+   LPSM+QQI 
Sbjct: 295 VLSRLAPFERGIYEDYVANFWCTTSILIKWKNLFTTQSLKSISLAATILASLPSMVQQIL 354

Query: 61  VPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLSIF 120
            PSN+GFLYGLL SS +FYLFSFQVHEKSIL+P L A++LAL  P  F H  +YAL S+F
Sbjct: 355 SPSNEGFLYGLLNSSMAFYLFSFQVHEKSILMPFLSATLLALKLPDHFSHLTYYALFSMF 414

Query: 121 PLVVRDKLVQAYLAIYALTFLII------NALNKGKQKGGGFHS--GGV-LFGCFLFCSL 180
           PL+ RDKL+  YL +  L  +I       +A+ K       F +  G V L     F S+
Sbjct: 415 PLLCRDKLLIPYLTLSFLFTVIYHSPGNHHAIQKTDVSFFSFKNFPGYVFLLRTHFFISV 474

Query: 181 VLHVVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQK 228
           VLHV+YL ++PP++YPFLFEA+IM+LCFS FI    ++N  QWTLS       K+K
Sbjct: 475 VLHVLYLTIKPPQKYPFLFEALIMILCFSYFIMFAFYTNYTQWTLSSHFGSSDKEK 530

[Close]

BLAST of Cucsa.031380 vs. NCBI nr
Match: gi|449455842|ref|XP_004145659.1| (PREDICTED: probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase [Cucumis sativus])

HSP 1 Score: 459.9 bits (1182), Expect = 2.6e-126
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 1


Query: 1   VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFSVKLLKILSFTATLSTCLPSMIQQIR 60
           VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFSVKLLKILSFTATLSTCLPSMIQQIR
Sbjct: 288 VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFSVKLLKILSFTATLSTCLPSMIQQIR 347

Query: 61  VPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLSIF 120
           VPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLSIF
Sbjct: 348 VPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLSIF 407

Query: 121 PLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLHVVYLVV 180
           PLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLHVVYLVV
Sbjct: 408 PLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLHVVYLVV 467

Query: 181 RPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN 230
           RPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN
Sbjct: 468 RPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN 516

[Close]

BLAST of Cucsa.031380 vs. NCBI nr
Match: gi|659123289|ref|XP_008461586.1| (PREDICTED: probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase [Cucumis melo])

HSP 1 Score: 434.5 bits (1116), Expect = 1.2e-118
Identity = 216/229 (94.32%), Postives = 221/229 (96.51%), Query Frame = 1


Query: 1   VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFSVKLLKILSFTATLSTCLPSMIQQIR 60
           VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFSVKLLKILSFTATLSTCLPSM+QQIR
Sbjct: 118 VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFSVKLLKILSFTATLSTCLPSMLQQIR 177

Query: 61  VPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLSIF 120
            PSNQGFL GLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLS+F
Sbjct: 178 FPSNQGFLCGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLSMF 237

Query: 121 PLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLHVVYLVV 180
           PLVVRDKLVQAYLAIYALTFLIINALN GKQK G FHS GVLFGC LFCSL+LHVVYLVV
Sbjct: 238 PLVVRDKLVQAYLAIYALTFLIINALNNGKQKRGVFHSAGVLFGCCLFCSLILHVVYLVV 297

Query: 181 RPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN 230
           RPPERYPFLFEA+IMLLCFSQFIFL+IF NVKQWTLSKAVPQMHKQKLN
Sbjct: 298 RPPERYPFLFEAIIMLLCFSQFIFLLIFFNVKQWTLSKAVPQMHKQKLN 346

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BLAST of Cucsa.031380 vs. NCBI nr
Match: gi|700202616|gb|KGN57749.1| (hypothetical protein Csa_3G271420 [Cucumis sativus])

HSP 1 Score: 352.1 bits (902), Expect = 7.6e-94
Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 1


Query: 55  MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY 114
           MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY
Sbjct: 1   MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY 60

Query: 115 ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLH 174
           ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLH
Sbjct: 61  ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLH 120

Query: 175 VVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN 230
           VVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN
Sbjct: 121 VVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN 175

[Close]

BLAST of Cucsa.031380 vs. NCBI nr
Match: gi|703084647|ref|XP_010092530.1| (putative dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase [Morus notabilis])

HSP 1 Score: 293.9 bits (751), Expect = 2.5e-76
Identity = 150/228 (65.79%), Postives = 173/228 (75.88%), Query Frame = 1


Query: 1   VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFSVKLLKILSFTATLSTCLPSMIQQIR 60
           VLSRLAPFERG+YEDYVANFWCTTSVLIKWKRLF+   LK+LSF AT+S CLPSM QQI 
Sbjct: 58  VLSRLAPFERGIYEDYVANFWCTTSVLIKWKRLFTTPSLKLLSFCATISACLPSMFQQIW 117

Query: 61  VPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLSIF 120
            PS QGFLYGLL S+FSFYLFSFQVHEKSILLPLLPAS+LA++EP +     HYAL S+F
Sbjct: 118 APSKQGFLYGLLNSAFSFYLFSFQVHEKSILLPLLPASLLAIEEPFVHSWMTHYALFSMF 177

Query: 121 PLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHS-GGVLFGCFLFCSLVLHVVYLV 180
           PLVVRDKLV  Y+A+  L FL   A     Q  G  +S    L   FLFC+L+LHVVY+ 
Sbjct: 178 PLVVRDKLVLPYIALSPLFFLFNRAGPTRAQATGEANSLRSFLTAFFLFCALILHVVYVT 237

Query: 181 VRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQK 228
           V PP RYPFLFEA+IMLLCFSQF+ L +++N KQW + K    + K K
Sbjct: 238 VNPPRRYPFLFEALIMLLCFSQFVSLTVYTNAKQWMMLKRPTLVDKDK 285

[Close]

BLAST of Cucsa.031380 vs. NCBI nr
Match: gi|225452021|ref|XP_002280181.1| (PREDICTED: probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase isoform X1 [Vitis vinifera])

HSP 1 Score: 290.0 bits (741), Expect = 3.5e-75
Identity = 150/231 (64.94%), Postives = 176/231 (76.19%), Query Frame = 1


Query: 1   VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFSVKLLKILSFTATLSTCLPSMIQQIR 60
           VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLF++K +K+LS  AT+ TCLPSMIQQI 
Sbjct: 284 VLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFTIKSMKLLSLWATILTCLPSMIQQIW 343

Query: 61  VPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLSIF 120
            PSN GFLYGLL SSFSFYLFSFQVHEKSILLPLLPA++LAL+EP  F    HYALLS+F
Sbjct: 344 SPSNLGFLYGLLNSSFSFYLFSFQVHEKSILLPLLPATLLALEEPFHFRWLTHYALLSMF 403

Query: 121 PLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVL----FGCFLFCSLVLHVV 180
           PL+ RDKL+  Y+A++AL +L+  A   G++      S   L        + CSL LH+V
Sbjct: 404 PLMCRDKLILPYVALFALFYLLYYAPG-GREDTRKMQSPSSLRSFMLSFLVLCSLFLHLV 463

Query: 181 YLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQK 228
           Y+ + PP++YPFLFEAVIMLLCFSQFI L I++N KQW L K    M K K
Sbjct: 464 YITLHPPDKYPFLFEAVIMLLCFSQFILLTIYTNAKQWMLLKHSTLMDKDK 513

The following BLAST results are available for this feature:

BLAST of Cucsa.031380 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ALG6_ARATH	1.9e-65	55.93	Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Ara...	[more]
ALG6_DICDI	7.2e-28	37.29	Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Dic...	[more]
ALG6_PONAB	3.6e-27	31.76	Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Pongo abelii...	[more]
ALG6_HUMAN	3.6e-27	31.76	Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Homo sapiens...	[more]
ALG6_CHICK	1.4e-26	36.05	Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Gallus gallu...	[more]

BLAST of Cucsa.031380 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9U3_CUCSA	5.3e-94	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G271420 PE=4 SV=1	[more]
W9R1J3_9ROSA	1.7e-76	65.79	Putative dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Mor...	[more]
D7TT36_VITVI	2.5e-75	64.94	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0006g03000 PE=4 SV=...	[more]
A0A0K9RNA8_SPIOL	2.1e-74	62.45	Uncharacterized protein OS=Spinacia oleracea GN=SOVF_053420 PE=4 SV=1	[more]
M5XQL1_PRUPE	2.7e-74	64.35	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004184mg PE=4 SV=1	[more]

BLAST of Cucsa.031380 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G38460.1	1.1e-66	55.93	ALG6, ALG8 glycosyltransferase family	[more]

BLAST of Cucsa.031380 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449455842\|ref\|XP_004145659.1\|	2.6e-126	100.00	PREDICTED: probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransfe...	[more]
gi\|659123289\|ref\|XP_008461586.1\|	1.2e-118	94.32	PREDICTED: probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransfe...	[more]
gi\|700202616\|gb\|KGN57749.1\|	7.6e-94	100.00	hypothetical protein Csa_3G271420 [Cucumis sativus]	[more]
gi\|703084647\|ref\|XP_010092530.1\|	2.5e-76	65.79	putative dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase [Morus...	[more]
gi\|225452021\|ref\|XP_002280181.1\|	3.5e-75	64.94	PREDICTED: probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransfe...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR004856	Glyco_trans_ALG6/ALG8

Vocabulary: **Molecular Function**
Term	Definition
GO:0016758	transferase activity, transferring hexosyl groups

Vocabulary: **Cellular Component**
Term	Definition
GO:0005789	endoplasmic reticulum membrane

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005789	endoplasmic reticulum membrane
molecular_function	GO:0016758	transferase activity, transferring hexosyl groups

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.031380.1	Cucsa.031380.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004856	Glycosyl transferase, ALG6/ALG8	PANTHER	PTHR12413	DOLICHYL GLYCOSYLTRANSFERASE	coord: 1..227 score: 3.6
IPR004856	Glycosyl transferase, ALG6/ALG8	PFAM	PF03155	Alg6_Alg8	coord: 1..206 score: 5.3
None	No IPR available	PANTHER	PTHR12413:SF1	DOLICHYL PYROPHOSPHATE MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE	coord: 1..227 score: 3.6

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.031380	Csa3G271420	Cucumber (Chinese Long) v2	cgycuB003
Cucsa.031380	MELO3C023794	Melon (DHL92) v3.5.1	cgymeB003
Cucsa.031380	ClCG05G011750	Watermelon (Charleston Gray)	cgywcgB004
Cucsa.031380	CSPI03G20640	Wild cucumber (PI 183967)	cgycpiB003
Cucsa.031380	CmaCh14G008090	Cucurbita maxima (Rimu)	cgycmaB0005
Cucsa.031380	Lsi05G003840	Bottle gourd (USVL1VR-Ls)	cgylsiB004
Cucsa.031380	MELO3C023794.2	Melon (DHL92) v3.6.1	cgymedB004
Cucsa.031380	Cla97C05G093770	Watermelon (97103) v2	cgywmbB004
Cucsa.031380	Bhi01G001460	Wax gourd	cgywgoB005
Cucsa.031380	Carg22274	Silver-seed gourd	carcgyB0552

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.031380	Silver-seed gourd	carcgyB0854
Cucsa.031380	Cucumber (Chinese Long) v3	cgycucB006
Cucsa.031380	Cucurbita maxima (Rimu)	cgycmaB0011
Cucsa.031380	Cucurbita moschata (Rifu)	cgycmoB0004
Cucsa.031380	Cucurbita moschata (Rifu)	cgycmoB0010
Cucsa.031380	Watermelon (97103) v1	cgywmB003
Cucsa.031380	Cucurbita pepo (Zucchini)	cgycpeB0008
Cucsa.031380	Cucurbita pepo (Zucchini)	cgycpeB0009