CSPI03G20640 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G20640
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Alpha-1,3-glucosyltransferase
Location	Chr3 : 16605458 .. 16606425 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTCTATCACGTCTTGCTCCCTTTGAGAGAGGATTGTATGAAGATTATGTGGCGAACTTTTGGTGTACCACGTCTGTCCTTATTAAGTGGAAGAGATTGTTTTCAGTGAAATTACTGAAAATTTTGAGCTTCACTGCTACATTATCAACTTGTTTACCTTCCATGATTCAACAGATTCGTGTCCCAAGTAACCAGGGGTTTCTATATGGCCTTTTATGTAGTTCTTTCTCATTCTACCTGTTCTCGTTTCAAGGTACTTGCTCTATCTCTCTCCCTTTTTTTCTCTCTCTCTCCACGAGCTAGGATAATATTTTTATGAAGATAGCCTAATTAACCGCCTCTGATTAACATTGCAGTGCATGAGAAGTCTATTCTTCTTCCTCTTCTTCCTGCAAGCATGTTGGCCCTGGATGAGCCTTCATTATTTATTCATTTTCTACACTATGCATTGCTTTCAATATTTCCTCTTGTAGTTCGAGACAAACTGGTTCAAGCATATCTTGCCATTTATGCTCTTACCTTTCTTATTATCAATGCATTAAATAAAGGAAAGCAAAAAGGGGGAGGATTTCATTCTGGGGGGGTTTTATTTGGATGCTTCCTGTTTTGTTCACTTGTTCTCCACGTTGTGTACTTGGTGGTGCGCCCTCCTGAGAGGTATCCCTTTCTCTTTGAGGCAGTAATTATGCTTCTTTGCTTTTCTCAATTCATCTTCCTTGTGATCTTCTCCAATGTAAAGCAATGGACGCTGTCTAAAGCTGTTCCACAGATGCACAAGCAAAAGCTTAATTAGTTTGATATGCTTTATAACTTAGTTACTTACCGTGACTTTACATTTTTGTTTGCTGAACCAATAACACAATATCGTGTTGAGCTTATTTGAAACAGAAGTAACTCAGCAGCGGCCCTACAGTAGTTCAATTGTTTTGATAATTGATGAAGTAACCGTTTCCAGTGAAACTTTTGTC

mRNA sequence

ATGATTCAACAGATTCGTGTCCCAAGTAACCAGGGGTTTCTATATGGCCTTTTATGTAGTTCTTTCTCATTCTACCTGTTCTCGTTTCAAGTGCATGAGAAGTCTATTCTTCTTCCTCTTCTTCCTGCAAGCATGTTGGCCCTGGATGAGCCTTCATTATTTATTCATTTTCTACACTATGCATTGCTTTCAATATTTCCTCTTGTAGTTCGAGACAAACTGGTTCAAGCATATCTTGCCATTTATGCTCTTACCTTTCTTATTATCAATGCATTAAATAAAGGAAAGCAAAAAGGGGGAGGATTTCATTCTGGGGGGGTTTTATTTGGATGCTTCCTGTTTTGTTCACTTGTTCTCCACGTTGTGTACTTGGTGGTGCGCCCTCCTGAGAGGTATCCCTTTCTCTTTGAGGCAGTAATTATGCTTCTTTGCTTTTCTCAATTCATCTTCCTTGTGATCTTCTCCAATGTAAAGCAATGGACGCTGTCTAAAGCTGTTCCACAGATGCACAAGCAAAAGCTTAATTAG

Coding sequence (CDS)

ATGATTCAACAGATTCGTGTCCCAAGTAACCAGGGGTTTCTATATGGCCTTTTATGTAGTTCTTTCTCATTCTACCTGTTCTCGTTTCAAGTGCATGAGAAGTCTATTCTTCTTCCTCTTCTTCCTGCAAGCATGTTGGCCCTGGATGAGCCTTCATTATTTATTCATTTTCTACACTATGCATTGCTTTCAATATTTCCTCTTGTAGTTCGAGACAAACTGGTTCAAGCATATCTTGCCATTTATGCTCTTACCTTTCTTATTATCAATGCATTAAATAAAGGAAAGCAAAAAGGGGGAGGATTTCATTCTGGGGGGGTTTTATTTGGATGCTTCCTGTTTTGTTCACTTGTTCTCCACGTTGTGTACTTGGTGGTGCGCCCTCCTGAGAGGTATCCCTTTCTCTTTGAGGCAGTAATTATGCTTCTTTGCTTTTCTCAATTCATCTTCCTTGTGATCTTCTCCAATGTAAAGCAATGGACGCTGTCTAAAGCTGTTCCACAGATGCACAAGCAAAAGCTTAATTAG

Homology

[Close]

BLAST of CSPI03G20640 vs. Swiss-Prot
Match: ALG6_ARATH (Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Arabidopsis thaliana GN=At5g38460 PE=2 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 2.0e-38
Identity = 93/182 (51.10%), Postives = 119/182 (65.38%), Query Frame = 1


Query: 1   MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY 60
           M+QQI  PSN+GFLYGLL SS +FYLFSFQVHEKSIL+P L A++LAL  P  F H  +Y
Sbjct: 349 MVQQILSPSNEGFLYGLLNSSMAFYLFSFQVHEKSILMPFLSATLLALKLPDHFSHLTYY 408

Query: 61  ALLSIFPLVVRDKLVQAYLAIYALTFLII------NALNKGKQKGGGFHS--GGV-LFGC 120
           AL S+FPL+ RDKL+  YL +  L  +I       +A+ K       F +  G V L   
Sbjct: 409 ALFSMFPLLCRDKLLIPYLTLSFLFTVIYHSPGNHHAIQKTDVSFFSFKNFPGYVFLLRT 468

Query: 121 FLFCSLVLHVVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHK 174
             F S+VLHV+YL ++PP++YPFLFEA+IM+LCFS FI    ++N  QWTLS       K
Sbjct: 469 HFFISVVLHVLYLTIKPPQKYPFLFEALIMILCFSYFIMFAFYTNYTQWTLSSHFGSSDK 528

[Close]

BLAST of CSPI03G20640 vs. Swiss-Prot
Match: ALG6_HUMAN (Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Homo sapiens GN=ALG6 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.8e-11
Identity = 50/178 (28.09%), Postives = 90/178 (50.56%), Query Frame = 1


Query: 2   IQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYA 61
           I+ I  PS++GF + L+  + SF+LFSFQVHEKSILL  LP  ++  + P +   FL  +
Sbjct: 314 IKLILQPSSKGFKFTLVSCALSFFLFSFQVHEKSILLVSLPVCLVLSEIPFMSTWFLLVS 373

Query: 62  LLSIFPLVVRDKLV-------QAYLAIYALTFLIINALNKGKQKGGGFH-SGGVLFGCFL 121
             S+ PL+++D+L+        A+      +F I    ++ + +   F  S      CF 
Sbjct: 374 TFSMLPLLLKDELLMPSVVTTMAFFIACVTSFSIFEKTSEEELQLKSFSISVRKYLPCFT 433

Query: 122 FCSLVLHVVYLV--------------VRPPERYPFLFEAVIMLLCFSQFIFLVIFSNV 158
           F S ++  ++L+              + PP++ P LF  ++  +    F+F +++ N+
Sbjct: 434 FLSRIIQYLFLISVITMVLLTLMTVTLDPPQKLPDLFSVLVCFVSCLNFLFFLVYFNI 491

[Close]

BLAST of CSPI03G20640 vs. Swiss-Prot
Match: ALG6_PONAB (Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Pongo abelii GN=ALG6 PE=2 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.8e-11
Identity = 50/178 (28.09%), Postives = 90/178 (50.56%), Query Frame = 1


Query: 2   IQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYA 61
           I+ I  PS++GF + L+  + SF+LFSFQVHEKSILL  LP  ++  + P +   FL  +
Sbjct: 314 IKLILQPSSKGFKFTLVSCALSFFLFSFQVHEKSILLVSLPVCLVLSEIPFMSTWFLLVS 373

Query: 62  LLSIFPLVVRDKLV-------QAYLAIYALTFLIINALNKGKQKGGGFH-SGGVLFGCFL 121
             S+ PL+++D+L+        A+      +F I    ++ + +   F  S      CF 
Sbjct: 374 TFSMLPLLLKDELLMPSVVTTMAFFIACVTSFSIFEKTSEEELQLKSFSISVRKYLPCFT 433

Query: 122 FCSLVLHVVYLV--------------VRPPERYPFLFEAVIMLLCFSQFIFLVIFSNV 158
           F S ++  ++L+              + PP++ P LF  ++  +    F+F +++ N+
Sbjct: 434 FLSRIIQYLFLISVITMVLLTLMTVTLGPPQKLPDLFSVLVCFVSCLNFLFFLVYFNI 491

[Close]

BLAST of CSPI03G20640 vs. Swiss-Prot
Match: ALG6_DICDI (Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Dictyostelium discoideum GN=alg6 PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.6e-11
Identity = 59/177 (33.33%), Postives = 90/177 (50.85%), Query Frame = 1


Query: 6   RVPSNQG-FLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLS 65
           R+P N+  F++ L+ SSFSF+LFSFQVHEK+ILL  LP S+L L  P++   F+  +  S
Sbjct: 333 RIPKNKFVFIHSLINSSFSFFLFSFQVHEKTILLVSLPISLLILHHPNMVWWFILISTFS 392

Query: 66  IFPLVVRDKLVQAYLAIYALTFL--------IINALNKGKQKGG----------GFHSGG 125
           +FPL+ +D LV  Y AI  L  +        I  + N+ K +             F+S  
Sbjct: 393 MFPLLFKDGLVIPYFAIMILYIVIGYQFKNSITRSNNQFKHQNSQENLLASSDKSFYSIY 452

Query: 126 VLFGCFL--FCSLVLHVVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWT 162
           + +  +L     +V H++Y     P   P L+  ++    F  FI L  F  +K+ T
Sbjct: 453 LNYWFYLNIIGMVVCHLLYQFAPHPPHLPSLWLLLVCNFSFIHFI-LTFFYFIKEMT 508

[Close]

BLAST of CSPI03G20640 vs. Swiss-Prot
Match: ALG6_DROME (Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Drosophila melanogaster GN=gny PE=2 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 3.0e-10
Identity = 53/154 (34.42%), Postives = 77/154 (50.00%), Query Frame = 1


Query: 9   SNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHYALLSIFPL 68
           +N GFL  L  +S +F+LFSFQVHEK+ILL  LPA  L    P   I FL   + S+ PL
Sbjct: 320 TNVGFLLALFNTSLAFFLFSFQVHEKTILLTALPALFLLKCWPDEMILFLEVTVFSMLPL 379

Query: 69  VVRDK-LVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLHVVYLVVR 128
           + RD+ LV A +A  A  F +I      K K    +    +        + + V  L V 
Sbjct: 380 LARDELLVPAVVATVA--FHLIFKCFDSKSKLSNEYPLKYIANISQILMISVVVASLTVP 439

Query: 129 PPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWT 162
            P +YP L+  +I +     F    ++ NV+Q++
Sbjct: 440 APTKYPDLWPLIISVTSCGHFFLFFLWGNVQQFS 471

[Close]

BLAST of CSPI03G20640 vs. TrEMBL
Match: A0A0A0L9U3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G271420 PE=4 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 1.1e-91
Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 1


Query: 1   MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY 60
           MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY
Sbjct: 1   MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY 60

Query: 61  ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLH 120
           ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLH
Sbjct: 61  ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLH 120

Query: 121 VVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN 176
           VVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN
Sbjct: 121 VVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN 175

[Close]

BLAST of CSPI03G20640 vs. TrEMBL
Match: W9R1J3_9ROSA (Putative dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Morus notabilis GN=L484_010418 PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 7.0e-46
Identity = 105/174 (60.34%), Postives = 124/174 (71.26%), Query Frame = 1


Query: 1   MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY 60
           M QQI  PS QGFLYGLL S+FSFYLFSFQVHEKSILLPLLPAS+LA++EP +     HY
Sbjct: 112 MFQQIWAPSKQGFLYGLLNSAFSFYLFSFQVHEKSILLPLLPASLLAIEEPFVHSWMTHY 171

Query: 61  ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHS-GGVLFGCFLFCSLVL 120
           AL S+FPLVVRDKLV  Y+A+  L FL   A     Q  G  +S    L   FLFC+L+L
Sbjct: 172 ALFSMFPLVVRDKLVLPYIALSPLFFLFNRAGPTRAQATGEANSLRSFLTAFFLFCALIL 231

Query: 121 HVVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQK 174
           HVVY+ V PP RYPFLFEA+IMLLCFSQF+ L +++N KQW + K    + K K
Sbjct: 232 HVVYVTVNPPRRYPFLFEALIMLLCFSQFVSLTVYTNAKQWMMLKRPTLVDKDK 285

[Close]

BLAST of CSPI03G20640 vs. TrEMBL
Match: D7TT36_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0006g03000 PE=4 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 1.7e-44
Identity = 105/177 (59.32%), Postives = 126/177 (71.19%), Query Frame = 1


Query: 1   MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY 60
           MIQQI  PSN GFLYGLL SSFSFYLFSFQVHEKSILLPLLPA++LAL+EP  F    HY
Sbjct: 338 MIQQIWSPSNLGFLYGLLNSSFSFYLFSFQVHEKSILLPLLPATLLALEEPFHFRWLTHY 397

Query: 61  ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVL----FGCFLFCS 120
           ALLS+FPL+ RDKL+  Y+A++AL +L+  A   G++      S   L        + CS
Sbjct: 398 ALLSMFPLMCRDKLILPYVALFALFYLLYYAPG-GREDTRKMQSPSSLRSFMLSFLVLCS 457

Query: 121 LVLHVVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQK 174
           L LH+VY+ + PP++YPFLFEAVIMLLCFSQFI L I++N KQW L K    M K K
Sbjct: 458 LFLHLVYITLHPPDKYPFLFEAVIMLLCFSQFILLTIYTNAKQWMLLKHSTLMDKDK 513

[Close]

BLAST of CSPI03G20640 vs. TrEMBL
Match: A0A0K9RNA8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_053420 PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 2.2e-44
Identity = 100/175 (57.14%), Postives = 131/175 (74.86%), Query Frame = 1


Query: 1   MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY 60
           MIQQI+ PSN+GFLY LL SSFSFYLFSFQVHEKSILLPLLPAS+LAL+EP +F   +H+
Sbjct: 339 MIQQIKDPSNKGFLYALLNSSFSFYLFSFQVHEKSILLPLLPASLLALEEPLVFRWLIHF 398

Query: 61  ALLSIFPLVVRDKLVQAYLAIYALTFLIINA-LNKGKQKGGGFHSGGVLFGCFL-FCSLV 120
            L S+FPL+ RD+LV AY+A+++L +L+  A   K   K   F +  +L    L FCS V
Sbjct: 399 GLFSMFPLLCRDQLVLAYIALFSLFYLLFYAPRKKDSGKIRSFFTLKLLITTLLVFCSFV 458

Query: 121 LHVVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQK 174
           LH+VYL + PP+RYPFLFEA+IML+ F+QF++L +++N KQW L K    + K+K
Sbjct: 459 LHIVYLAMNPPKRYPFLFEAIIMLVSFTQFVWLALYTNKKQWMLQKQSTCVEKEK 513

[Close]

BLAST of CSPI03G20640 vs. TrEMBL
Match: A0A0L9V7Y5_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan08g201700 PE=4 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 3.8e-44
Identity = 98/167 (58.68%), Postives = 125/167 (74.85%), Query Frame = 1


Query: 1   MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY 60
           MIQQI+ PS++GFLY LL SSFSFYLFSFQVHEKSILLPLLPA++LA++EP +F  F  +
Sbjct: 337 MIQQIKSPSHRGFLYALLNSSFSFYLFSFQVHEKSILLPLLPATLLAVEEPFIFKWFTQF 396

Query: 61  ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGF---HSGGVLFGCFLFCSL 120
           A+LS+FPL+ RD LV AYLA+ AL  LI+NA  + K +   +   + G       L CS 
Sbjct: 397 AMLSMFPLICRDNLVVAYLALLALFVLILNAPVQHKVRETNYLNSYLGSATMFFILCCSF 456

Query: 121 VLHVVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSK 165
           VLH+VYL + PPE+YPFLFEA+IM LCFSQF+ + +  N+KQW L+K
Sbjct: 457 VLHIVYLTMHPPEKYPFLFEAIIMNLCFSQFVLVTLACNIKQWLLNK 503

[Close]

BLAST of CSPI03G20640 vs. TAIR10
Match: AT5G38460.1 (AT5G38460.1 ALG6, ALG8 glycosyltransferase family)

HSP 1 Score: 160.2 bits (404), Expect = 1.1e-39
Identity = 93/182 (51.10%), Postives = 119/182 (65.38%), Query Frame = 1


Query: 1   MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY 60
           M+QQI  PSN+GFLYGLL SS +FYLFSFQVHEKSIL+P L A++LAL  P  F H  +Y
Sbjct: 349 MVQQILSPSNEGFLYGLLNSSMAFYLFSFQVHEKSILMPFLSATLLALKLPDHFSHLTYY 408

Query: 61  ALLSIFPLVVRDKLVQAYLAIYALTFLII------NALNKGKQKGGGFHS--GGV-LFGC 120
           AL S+FPL+ RDKL+  YL +  L  +I       +A+ K       F +  G V L   
Sbjct: 409 ALFSMFPLLCRDKLLIPYLTLSFLFTVIYHSPGNHHAIQKTDVSFFSFKNFPGYVFLLRT 468

Query: 121 FLFCSLVLHVVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHK 174
             F S+VLHV+YL ++PP++YPFLFEA+IM+LCFS FI    ++N  QWTLS       K
Sbjct: 469 HFFISVVLHVLYLTIKPPQKYPFLFEALIMILCFSYFIMFAFYTNYTQWTLSSHFGSSDK 528

[Close]

BLAST of CSPI03G20640 vs. NCBI nr
Match: gi|449455842|ref|XP_004145659.1| (PREDICTED: probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase [Cucumis sativus])

HSP 1 Score: 344.0 bits (881), Expect = 1.6e-91
Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 1


Query: 1   MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY 60
           MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY
Sbjct: 342 MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY 401

Query: 61  ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLH 120
           ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLH
Sbjct: 402 ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLH 461

Query: 121 VVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN 176
           VVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN
Sbjct: 462 VVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN 516

[Close]

BLAST of CSPI03G20640 vs. NCBI nr
Match: gi|700202616|gb|KGN57749.1| (hypothetical protein Csa_3G271420 [Cucumis sativus])

HSP 1 Score: 344.0 bits (881), Expect = 1.6e-91
Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 1


Query: 1   MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY 60
           MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY
Sbjct: 1   MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY 60

Query: 61  ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLH 120
           ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLH
Sbjct: 61  ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLH 120

Query: 121 VVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN 176
           VVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN
Sbjct: 121 VVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN 175

[Close]

BLAST of CSPI03G20640 vs. NCBI nr
Match: gi|659123289|ref|XP_008461586.1| (PREDICTED: probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase [Cucumis melo])

HSP 1 Score: 318.5 bits (815), Expect = 7.1e-84
Identity = 162/175 (92.57%), Postives = 167/175 (95.43%), Query Frame = 1


Query: 1   MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY 60
           M+QQIR PSNQGFL GLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY
Sbjct: 172 MLQQIRFPSNQGFLCGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY 231

Query: 61  ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHSGGVLFGCFLFCSLVLH 120
           ALLS+FPLVVRDKLVQAYLAIYALTFLIINALN GKQK G FHS GVLFGC LFCSL+LH
Sbjct: 232 ALLSMFPLVVRDKLVQAYLAIYALTFLIINALNNGKQKRGVFHSAGVLFGCCLFCSLILH 291

Query: 121 VVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQKLN 176
           VVYLVVRPPERYPFLFEA+IMLLCFSQFIFL+IF NVKQWTLSKAVPQMHKQKLN
Sbjct: 292 VVYLVVRPPERYPFLFEAIIMLLCFSQFIFLLIFFNVKQWTLSKAVPQMHKQKLN 346

[Close]

BLAST of CSPI03G20640 vs. NCBI nr
Match: gi|502180608|ref|XP_004516676.1| (PREDICTED: probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase [Cicer arietinum])

HSP 1 Score: 193.4 bits (490), Expect = 3.4e-46
Identity = 98/167 (58.68%), Postives = 129/167 (77.25%), Query Frame = 1


Query: 1   MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY 60
           M+QQI+ PSNQGFLYGLL SS SFYLFSFQVHEKSILLPLLPA++LA++EP +F  F+ +
Sbjct: 340 MVQQIKSPSNQGFLYGLLNSSLSFYLFSFQVHEKSILLPLLPATLLAMEEPFIFRWFMQF 399

Query: 61  ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHS---GGVLFGCFLFCSL 120
           A+LS+FPL+VRD L+  Y A++AL FL++NA  + K +   F S   G       L CSL
Sbjct: 400 AMLSMFPLMVRDGLIIPYFALFALFFLVLNAPGQQKVRDNNFLSNYLGSTSMHLILCCSL 459

Query: 121 VLHVVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSK 165
           +LH+VYL ++PP++YPFLFEA+IM LCFSQF+ + +  N+KQW L+K
Sbjct: 460 ILHIVYLTMQPPKKYPFLFEAMIMNLCFSQFVIVTLGYNIKQWMLNK 506

[Close]

BLAST of CSPI03G20640 vs. NCBI nr
Match: gi|703084647|ref|XP_010092530.1| (putative dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase [Morus notabilis])

HSP 1 Score: 191.8 bits (486), Expect = 1.0e-45
Identity = 105/174 (60.34%), Postives = 124/174 (71.26%), Query Frame = 1


Query: 1   MIQQIRVPSNQGFLYGLLCSSFSFYLFSFQVHEKSILLPLLPASMLALDEPSLFIHFLHY 60
           M QQI  PS QGFLYGLL S+FSFYLFSFQVHEKSILLPLLPAS+LA++EP +     HY
Sbjct: 112 MFQQIWAPSKQGFLYGLLNSAFSFYLFSFQVHEKSILLPLLPASLLAIEEPFVHSWMTHY 171

Query: 61  ALLSIFPLVVRDKLVQAYLAIYALTFLIINALNKGKQKGGGFHS-GGVLFGCFLFCSLVL 120
           AL S+FPLVVRDKLV  Y+A+  L FL   A     Q  G  +S    L   FLFC+L+L
Sbjct: 172 ALFSMFPLVVRDKLVLPYIALSPLFFLFNRAGPTRAQATGEANSLRSFLTAFFLFCALIL 231

Query: 121 HVVYLVVRPPERYPFLFEAVIMLLCFSQFIFLVIFSNVKQWTLSKAVPQMHKQK 174
           HVVY+ V PP RYPFLFEA+IMLLCFSQF+ L +++N KQW + K    + K K
Sbjct: 232 HVVYVTVNPPRRYPFLFEALIMLLCFSQFVSLTVYTNAKQWMMLKRPTLVDKDK 285

The following BLAST results are available for this feature:

BLAST of CSPI03G20640 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ALG6_ARATH	2.0e-38	51.10	Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Ara...	[more]
ALG6_HUMAN	2.8e-11	28.09	Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Homo sapiens...	[more]
ALG6_PONAB	2.8e-11	28.09	Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Pongo abelii...	[more]
ALG6_DICDI	3.6e-11	33.33	Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Dic...	[more]
ALG6_DROME	3.0e-10	34.42	Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Dro...	[more]

BLAST of CSPI03G20640 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9U3_CUCSA	1.1e-91	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G271420 PE=4 SV=1	[more]
W9R1J3_9ROSA	7.0e-46	60.34	Putative dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Mor...	[more]
D7TT36_VITVI	1.7e-44	59.32	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0006g03000 PE=4 SV=...	[more]
A0A0K9RNA8_SPIOL	2.2e-44	57.14	Uncharacterized protein OS=Spinacia oleracea GN=SOVF_053420 PE=4 SV=1	[more]
A0A0L9V7Y5_PHAAN	3.8e-44	58.68	Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan08g201700 PE=4 SV=1	[more]

BLAST of CSPI03G20640 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G38460.1	1.1e-39	51.10	ALG6, ALG8 glycosyltransferase family	[more]

BLAST of CSPI03G20640 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449455842\|ref\|XP_004145659.1\|	1.6e-91	100.00	PREDICTED: probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransfe...	[more]
gi\|700202616\|gb\|KGN57749.1\|	1.6e-91	100.00	hypothetical protein Csa_3G271420 [Cucumis sativus]	[more]
gi\|659123289\|ref\|XP_008461586.1\|	7.1e-84	92.57	PREDICTED: probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransfe...	[more]
gi\|502180608\|ref\|XP_004516676.1\|	3.4e-46	58.68	PREDICTED: probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransfe...	[more]
gi\|703084647\|ref\|XP_010092530.1\|	1.0e-45	60.34	putative dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase [Morus...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR004856	Glyco_trans_ALG6/ALG8

Vocabulary: **Molecular Function**
Term	Definition
GO:0016758	transferase activity, transferring hexosyl groups

Vocabulary: **Cellular Component**
Term	Definition
GO:0005789	endoplasmic reticulum membrane

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
biological_process	GO:0006486	protein glycosylation
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016758	transferase activity, transferring hexosyl groups

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G20640.1	CSPI03G20640.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004856	Glycosyl transferase, ALG6/ALG8	PANTHER	PTHR12413	DOLICHYL GLYCOSYLTRANSFERASE	coord: 1..173 score: 1.2
IPR004856	Glycosyl transferase, ALG6/ALG8	PFAM	PF03155	Alg6_Alg8	coord: 2..152 score: 9.9
None	No IPR available	PANTHER	PTHR12413:SF1	DOLICHYL PYROPHOSPHATE MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE	coord: 1..173 score: 1.2

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI03G20640	Csa3G271420	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G20640	MELO3C023794	Melon (DHL92) v3.5.1	cpimeB243
CSPI03G20640	Cucsa.031380	Cucumber (Gy14) v1	cgycpiB003
CSPI03G20640	CsGy3G020320	Cucumber (Gy14) v2	cgybcpiB110

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G20640	Melon (DHL92) v3.6.1	cpimedB229
CSPI03G20640	Silver-seed gourd	carcpiB0520
CSPI03G20640	Silver-seed gourd	carcpiB0798
CSPI03G20640	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G20640	Watermelon (97103) v2	cpiwmbB219
CSPI03G20640	Watermelon (97103) v2	cpiwmbB224
CSPI03G20640	Wax gourd	cpiwgoB271
CSPI03G20640	Cucurbita maxima (Rimu)	cmacpiB244
CSPI03G20640	Cucurbita maxima (Rimu)	cmacpiB458
CSPI03G20640	Cucurbita moschata (Rifu)	cmocpiB232
CSPI03G20640	Cucurbita moschata (Rifu)	cmocpiB446
CSPI03G20640	Watermelon (Charleston Gray)	cpiwcgB236
CSPI03G20640	Watermelon (Charleston Gray)	cpiwcgB241
CSPI03G20640	Watermelon (97103) v1	cpiwmB246
CSPI03G20640	Cucurbita pepo (Zucchini)	cpecpiB550
CSPI03G20640	Cucurbita pepo (Zucchini)	cpecpiB602
CSPI03G20640	Bottle gourd (USVL1VR-Ls)	cpilsiB210