Cucsa.031370 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.031370 vs. Swiss-Prot
Match: C7351_ARATH (Cytokinin hydroxylase OS=Arabidopsis thaliana GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 1.1e-209
Identity = 353/519 (68.02%), Postives = 427/519 (82.27%), Query Frame = 1

Query: 3   IMIILTTLLVIFISSLAKIAYDTILCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDVAA 62
           ++ IL +LLVIF++++ ++ YDTI CYWLTPRRI+K ME+QGV GP PRPL GNIL+++A
Sbjct: 2   LLTILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISA 61

Query: 63  LLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVLSK 122
           ++ +S +KD  SI H+IV RLLPHY  W+KQYGKRFI WNG +PRLCL ETELIKE+L K
Sbjct: 62  MVSQSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMK 121

Query: 123 NSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYVGLMVECTNK 182
           ++ V GRSWLQ+QGTK+FIG+GLLMANG+DW+HQRH+ AP+F G++LK Y   MVECT+K
Sbjct: 122 HNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSK 181

Query: 183 LIQSLETEFDSGRTEFEIADYMKQLTANIISRTEFDCNSDKGNQIFHLLTLLQHLCAQAS 242
           L++ L  E   G  E EI + M +LTA+IISRT+F  + +KG ++F+ LT+LQ  CAQA+
Sbjct: 182 LVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQAT 241

Query: 243 RHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNE 302
           RHLC PGSRF PSKYNREIKSLK EVERLL+EIIQSR+D  E+GRS+ +G DLLG+LLNE
Sbjct: 242 RHLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNE 301

Query: 303 MQRRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPKWQQKVRHEVN 362
           M   + + +N  + NLQLIMDECKTFFFAGHETTALLLTWT MLLA NP WQ+KVR EV 
Sbjct: 302 MDIDKNNNNN--NNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVR 361

Query: 363 QVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWI 422
           +V      PSV+ LSK T L+ VINESLRLYPPAT+LPRMAFED+K+GDL IPKGLSIWI
Sbjct: 362 EVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWI 421

Query: 423 PVLAIHHSEELWGKDVNEFKPERFASKPFSGGR-FIPFAAGPRNCIGQGFALMEAKIILA 482
           PVLAIHHSEELWGKD N+F PERF  +PF+ GR FIPFAAGPRNCIGQ FALMEAKIILA
Sbjct: 422 PVLAIHHSEELWGKDANQFNPERFGGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILA 481

Query: 483 MLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINS 521
            L+SKF+F+IS +YRHAP+ VLTI+PKYGVQV L P+ S
Sbjct: 482 TLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPLVS 518

BLAST of Cucsa.031370 vs. Swiss-Prot
Match: C7352_ARATH (Cytokinin hydroxylase OS=Arabidopsis thaliana GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 1.7e-202
Identity = 346/520 (66.54%), Postives = 415/520 (79.81%), Query Frame = 1

Query: 1   MAIMIILTTLLVIFISSLAKIAYDTILCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60
           M + ++L  +LVI ++ + ++ YD+I CY+LTPRRI+K ME+QG+ GP PR L GNI+D+
Sbjct: 1   MMVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDI 60

Query: 61  AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120
           + +L  S + D SSI HNIV RLLPHY +W+KQYGKRFI WNG EPRLCL ETE+IKE+L
Sbjct: 61  SKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELL 120

Query: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYVGLMVECT 180
           +K++ V G+SWLQ+QGTK FIG+GLLMANGE W+HQRH+ AP+F  D+LK Y   MVECT
Sbjct: 121 TKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECT 180

Query: 181 NKLIQSLETEFDSGRTEFEIADYMKQLTANIISRTEFDCNSDKGNQIFHLLTLLQHLCAQ 240
             + + L  E      E EI + M++LTA+IISRTEF  + DKG ++F LLT+LQ LCAQ
Sbjct: 181 KMMAERLRKEVGE---EVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300
           A+RHLC PGSRF PSKYNREIKSLK EVERLLMEII SRKD VEIGRS+ YG DLLG+LL
Sbjct: 241 ATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLL 300

Query: 301 NEMQRRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPKWQQKVRHE 360
           N+M        N  +LN+Q+IMDECKTFFF GHETT+LLLTWT+MLLA NP WQ  VR E
Sbjct: 301 NQMD------SNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDE 360

Query: 361 VNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSI 420
           V QVC     PSVE LS  T LN VINESLRLYPPAT+LPRMAFEDIK+GDL IPKGLSI
Sbjct: 361 VRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSI 420

Query: 421 WIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGR-FIPFAAGPRNCIGQGFALMEAKII 480
           WIPVLAIHHS ELWG+D NEF PERF ++ F+  R F+PFAAGPRNCIGQ FA+MEAKII
Sbjct: 421 WIPVLAIHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKII 480

Query: 481 LAMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPIN 520
           LAML+SKFSF+IS++YRHAP+ VLTI+PKYGVQ+ L P++
Sbjct: 481 LAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of Cucsa.031370 vs. Swiss-Prot
Match: C70B2_ARATH (Cytochrome P450 709B2 OS=Arabidopsis thaliana GN=CYP709B2 PE=2 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 1.8e-95
Identity = 195/525 (37.14%), Postives = 292/525 (55.62%), Query Frame = 1

Query: 1   MAIMIILTTLLVIFISSLAKIAYDTILCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60
           M ++  +  L +  +  +    Y         P  + ++ +KQG+ GP  R L GN+ ++
Sbjct: 56  MELLSTINLLAIALVLLVVPKIYGACRILVWRPWMLSRRFKKQGISGPKYRILYGNLREI 115

Query: 61  AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120
             +  ++    +    ++IV R+LPH   W  QYG+ F+YW G +PRLC+++ EL K++L
Sbjct: 116 RKMKNEAKLMVLDPNSNDIVPRVLPHLQQWKSQYGETFLYWQGTDPRLCISDHELAKQIL 175

Query: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYVGLMVECT 180
           S       +S  + +  K   G GL+  NG DW   R I+ P+F  DKLK    LMV+CT
Sbjct: 176 SNKFVFFSKSKTKPEILK-LSGNGLIFVNGLDWVRHRRILNPAFSMDKLKLMTQLMVDCT 235

Query: 181 NKLIQSLETEFDSGRTE--FEIADYMKQLTANIISRTEFDCNSDKGNQIFHLLTLLQHLC 240
            ++    + + +   TE    I+   K+LTA+II+   F  +  +G ++F     LQ  C
Sbjct: 236 FRMFLEWKKQRNGVETEQFVLISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCC 295

Query: 241 AQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGM 300
           A A   L  PG ++ P+  N +I  L M+V   +  II +R        S  YG+DLLG+
Sbjct: 296 AAALTDLYFPGIQYLPTPSNLQIWKLDMKVNSSIKRIIDARLTS----ESKDYGNDLLGI 355

Query: 301 LLNEMQRRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPKWQQKVR 360
           +L        +      +++  I++ECKTFFFAGHETTA LLTW+ MLL+ +  WQ+K+R
Sbjct: 356 MLTAASSNESEK----KMSIDEIIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLR 415

Query: 361 HEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGL 420
            EV   C     P  E  SK  L+N V  ESLRLY P   L R+A ED+K+G+LEIPKG 
Sbjct: 416 EEVFNECGKDKIPDAETCSKLKLMNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKGT 475

Query: 421 SIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGR----FIPFAAGPRNCIGQGFALM 480
           +I +P+  +H  + +WG D ++F P RFA+            + F+ GPR CIGQ FA+M
Sbjct: 476 TIILPIAKMHRDKAVWGSDADKFNPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAIM 535

Query: 481 EAKIILAMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPIN 520
           EAK +LAM+L +F  ++S  Y+HAP   LT+QP+Y + V L PI+
Sbjct: 536 EAKTVLAMILQRFRLNLSADYKHAPADHLTLQPQYDLPVILEPID 571

BLAST of Cucsa.031370 vs. Swiss-Prot
Match: C7091_ARATH (Cytochrome P450 709B1 OS=Arabidopsis thaliana GN=CYP709B1 PE=2 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 4.1e-95
Identity = 191/528 (36.17%), Postives = 301/528 (57.01%), Query Frame = 1

Query: 1   MAIMIILTTLLVIFISSLAKIAYDTILCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60
           M ++I L  +++I I  L +I    ++  W  P  + ++++ QG+ GP  R   GN+ ++
Sbjct: 1   MGLVIFLALIVLILIIGL-RIFKAFMILVW-HPFVLTRRLKNQGISGPNYRIFYGNLSEI 60

Query: 61  AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120
             +  +S    +    ++I  R+LPHY  W  QYG+ F+YWNG EPR+C+++ EL K +L
Sbjct: 61  KKMKRESHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKTML 120

Query: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYVGLMVECT 180
           S       +S  + +  K    KGL+   G DW   R I+ P+F  D+LK    +MV+CT
Sbjct: 121 SNKLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVDCT 180

Query: 181 NKLIQSLETEFDSGRTEF-----EIADYMKQLTANIISRTEFDCNSDKGNQIFHLLTLLQ 240
            K+++    E     TE      E+ +  ++LTA+II+ + F  +  +G ++F     L+
Sbjct: 181 LKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMELK 240

Query: 241 HLCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDL 300
                +   + +PG+++ P+  N  +  L+ +++  +  II SR     +   + YG DL
Sbjct: 241 RCYTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSR-----LQSKSDYGDDL 300

Query: 301 LGMLLNEMQRRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPKWQQ 360
           LG+LL       ++      ++++ I+ EC+TFFF GHETT+ LL WT MLL+ +  WQ+
Sbjct: 301 LGILLKAYNTEGKER----KMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQE 360

Query: 361 KVRHEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIP 420
           K+R E+ + C    TP  E  SK  L+NMVI ESLRLY P + L R A  +IK+GDLEIP
Sbjct: 361 KLREEIFKECGKEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEIP 420

Query: 421 KGLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGR----FIPFAAGPRNCIGQGF 480
           KG ++ IP+L +H  + LWG D ++F P RFA+            + F+ GPR CIGQ F
Sbjct: 421 KGTTVVIPLLKMHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPRACIGQNF 480

Query: 481 ALMEAKIILAMLLSKFSF-SISDSYRHAPVSVLTIQPKYGVQVYLTPI 519
            ++EAK +L M+L +F F S+ D Y+H PV  +TIQP+YG+ V L P+
Sbjct: 481 VMIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPL 517

BLAST of Cucsa.031370 vs. Swiss-Prot
Match: C734A_ARATH (Cytochrome P450 734A1 OS=Arabidopsis thaliana GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.4e-90
Identity = 174/502 (34.66%), Postives = 288/502 (57.37%), Query Frame = 1

Query: 30  WLTPRRIRKKMEKQGVRGPVPRPLVGNILDVAALLGKSTAKDMSSIDHNIVDRLLPHYTT 89
           W  PR+I +   KQG+RGP     +GN+ ++  ++ K+++  M    HNI+ R+L  Y  
Sbjct: 32  WWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASSHPMP-FSHNILPRVLSFYHH 91

Query: 90  WTKQYGKRFIYWNGMEPRLCLAETELIKEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMAN 149
           W K YG  F+ W G   RL +A+ +LI+E+ SK+              K   G GLL   
Sbjct: 92  WRKIYGATFLVWFGPTFRLTVADPDLIREIFSKSEFYEKNE--AHPLVKQLEGDGLLSLK 151

Query: 150 GEDWYHQRHIVAPSFMGDKLKSYVGLMVECTNKLIQSLETEF-DSGRTEFEIADYMKQLT 209
           GE W H R I++P+F  + LK  V ++++    ++     +  ++G  E ++ ++ + LT
Sbjct: 152 GEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSDKLSENGEVEVDVYEWFQILT 211

Query: 210 ANIISRTEFDCNSDKGNQIFHLLTLLQHLCAQASRHLCLPGSRFFPSKYNREIKSLKMEV 269
            ++ISRT F  + + G  +F L      LCA+A + + +PG RFFP++ N +   L  E+
Sbjct: 212 EDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIPGYRFFPTRGNLKSWKLDKEI 271

Query: 270 ERLLMEIIQSRKDGVEIGRST-CY---GSDLLGMLLNEMQRRREDGDNGFSLNLQLIMDE 329
            + L+++I+ R+     G    C      DLLG+++              ++ +Q I++E
Sbjct: 272 RKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQAK-----------NVTVQDIVEE 331

Query: 330 CKTFFFAGHETTALLLTWTIMLLATNPKWQQKVRHEVNQVCHNGATPSVEHLSKFTLLNM 389
           CK+FFFAG +TT+ LLTWT +LL+ +P+WQ K R EV +VC +   P+ +H+ K   L+M
Sbjct: 332 CKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGSRDVPTKDHVVKLKTLSM 391

Query: 390 VINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGKDVNEFKPE 449
           ++NESLRLYPP     R A  D+K+G  +IP G  + IP++A+HH + +WG DVNEF P 
Sbjct: 392 ILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAVHHDQAIWGNDVNEFNPA 451

Query: 450 RFASKPFSGGR----FIPFAAGPRNCIGQGFALMEAKIILAMLLSKFSFSISDSYRHAPV 509
           RFA       +    FIPF  G R CIGQ  A+++AK+ LA+++ +F+F ++ +Y+HAP 
Sbjct: 452 RFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVMIQRFTFHLAPTYQHAPT 511

Query: 510 SVLTIQPKYGVQVYLTPINSYQ 523
            ++ + P++G  +    + +++
Sbjct: 512 VLMLLYPQHGAPITFRRLTNHE 519

BLAST of Cucsa.031370 vs. TrEMBL
Match: A0A0A0L6Y6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G271920 PE=3 SV=1)

HSP 1 Score: 1053.1 bits (2722), Expect = 1.1e-304
Identity = 518/523 (99.04%), Postives = 520/523 (99.43%), Query Frame = 1

Query: 1   MAIMIILTTLLVIFISSLAKIAYDTILCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60
           MAIMIILTTLLVIFISSLAKIAYDTI CYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV
Sbjct: 1   MAIMIILTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61  AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120
           AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL
Sbjct: 61  AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120

Query: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYVGLMVECT 180
           SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSY GLMVECT
Sbjct: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLMVECT 180

Query: 181 NKLIQSLETEFDSGRTEFEIADYMKQLTANIISRTEFDCNSDKGNQIFHLLTLLQHLCAQ 240
           NKLIQSLETEFDSGRTEFEIADYMKQLTA+IISRTEFDCNSDKGNQIFHLLTLLQHLCAQ
Sbjct: 181 NKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDCNSDKGNQIFHLLTLLQHLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300
           ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL
Sbjct: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300

Query: 301 NEMQRRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPKWQQKVRHE 360
           NEMQ+RREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNP WQQKVRHE
Sbjct: 301 NEMQKRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHE 360

Query: 361 VNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSI 420
           VNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSI
Sbjct: 361 VNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSI 420

Query: 421 WIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKIIL 480
           WIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKIIL
Sbjct: 421 WIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKIIL 480

Query: 481 AMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINSYQP 524
           AMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINSYQP
Sbjct: 481 AMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINSYQP 523

BLAST of Cucsa.031370 vs. TrEMBL
Match: B9RSK4_RICCO (Cytochrome P450, putative OS=Ricinus communis GN=RCOM_1725340 PE=3 SV=1)

HSP 1 Score: 830.5 bits (2144), Expect = 1.2e-237
Identity = 407/518 (78.57%), Postives = 459/518 (88.61%), Query Frame = 1

Query: 4   MIILTTLLVIFISSLAKIAYDTILCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDVAAL 63
           M +LTT+LVI  S   K+AYDTI CY LTPRRI+K MEKQGVRGP PRPL GNILDV AL
Sbjct: 1   MAVLTTVLVIVFSLFFKVAYDTISCYLLTPRRIKKIMEKQGVRGPQPRPLTGNILDVTAL 60

Query: 64  LGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVLSKN 123
           + +ST++D   + H+IV RLLPH+ TW+KQYGKRFIYWNG+EPR+CL ETELIKE+L+K 
Sbjct: 61  VSQSTSRDCDYVHHDIVYRLLPHFVTWSKQYGKRFIYWNGIEPRMCLTETELIKELLTKY 120

Query: 124 SNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYVGLMVECTNKL 183
           S   G+SWLQ+QG+KHFIG+GLLMANG++WYHQRHIVAP+FMGD+LKSY G MVECT K+
Sbjct: 121 STKAGKSWLQQQGSKHFIGRGLLMANGDNWYHQRHIVAPAFMGDRLKSYSGHMVECTKKM 180

Query: 184 IQSLETEFDSGRTEFEIADYMKQLTANIISRTEFDCNSDKGNQIFHLLTLLQHLCAQASR 243
           +QSL+   +SG+TEFEI +YM +LTA+IISRTEFDC+ +KG QIFHLL +LQ LCAQASR
Sbjct: 181 LQSLQKAVESGQTEFEIGEYMTRLTADIISRTEFDCSYEKGKQIFHLLNVLQRLCAQASR 240

Query: 244 HLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEM 303
           HLC PGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKD VEIGRS+ YG+DLLGMLLNEM
Sbjct: 241 HLCFPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDCVEIGRSSTYGNDLLGMLLNEM 300

Query: 304 QRRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPKWQQKVRHEVNQ 363
           Q +RE   NGFSLNLQLIMDECKTFFFAGHETTALLLTWT+MLLA+NP WQ+KVR EVNQ
Sbjct: 301 QSKRE---NGFSLNLQLIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQEKVRAEVNQ 360

Query: 364 VCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIP 423
           VC NG TPSV+HL K TLLNMVINESLRLYPPATVLPRMAFEDIK+GDL IPKGLSIWIP
Sbjct: 361 VC-NGETPSVDHLPKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLHIPKGLSIWIP 420

Query: 424 VLAIHHSEELWGKDVNEFKPERFASKPFSGGR-FIPFAAGPRNCIGQGFALMEAKIILAM 483
           VLAIHHSEELWGKD NEF P+RFASK F+ GR FIPFAAGPRNCIGQ FA+MEAKIILAM
Sbjct: 421 VLAIHHSEELWGKDANEFNPDRFASKTFAPGRHFIPFAAGPRNCIGQTFAMMEAKIILAM 480

Query: 484 LLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINS 521
           L+SKF+F+ISDSYRHAPV VLTI+PKYGVQV L P+N+
Sbjct: 481 LISKFNFNISDSYRHAPVIVLTIKPKYGVQVCLRPLNN 514

BLAST of Cucsa.031370 vs. TrEMBL
Match: M5WVP1_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa018639mg PE=3 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 4.4e-237
Identity = 404/516 (78.29%), Postives = 461/516 (89.34%), Query Frame = 1

Query: 5   IILTTLLVIFISSLAKIAYDTILCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDVAALL 64
           ++LTTLLVI +S L ++AYDT+ CY+LTPRRI+K MEKQGVRGP PRPL GNILD+A+L+
Sbjct: 4   MLLTTLLVILLSLLIRVAYDTLSCYFLTPRRIKKIMEKQGVRGPKPRPLNGNILDMASLV 63

Query: 65  GKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVLSKNS 124
            KST+ DM +I H+IV RLLPHY  W+KQYGKRFIYW G+EPR+CLAETELIKE+LSK S
Sbjct: 64  AKSTSHDMHTISHDIVGRLLPHYVLWSKQYGKRFIYWTGIEPRMCLAETELIKELLSKYS 123

Query: 125 NVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYVGLMVECTNKLI 184
            V G+SWLQ+QG+KHFIG+GLLMANGEDWYHQRHIVAP+FMGD+LKSY G MVECT +++
Sbjct: 124 TVSGKSWLQQQGSKHFIGRGLLMANGEDWYHQRHIVAPAFMGDRLKSYAGYMVECTKEML 183

Query: 185 QSLETEFD-SGRTEFEIADYMKQLTANIISRTEFDCNSDKGNQIFHLLTLLQHLCAQASR 244
           QSL+ E + SG  EFEI +YM +LTA+IISRTEFD + +KG QIF LLT+LQHLC+QAS+
Sbjct: 184 QSLQNEIETSGGREFEIGEYMTRLTADIISRTEFDSSYEKGKQIFRLLTVLQHLCSQASK 243

Query: 245 HLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEM 304
           HLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKD VEIGRS+ YG+DLLGMLLNEM
Sbjct: 244 HLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDCVEIGRSSSYGNDLLGMLLNEM 303

Query: 305 QRRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPKWQQKVRHEVNQ 364
           Q++R    NGFS+NLQLIMDECKTFFFAGHETTALLLTWT+MLLA+NP WQ+KVR E+ Q
Sbjct: 304 QKKR---GNGFSINLQLIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQEKVRAELKQ 363

Query: 365 VCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIP 424
           VC NG TPSV+HL K TLLNMVINESLRLYPPATVLPRMAFEDIK+GDL IPKGLSIWIP
Sbjct: 364 VC-NGGTPSVDHLPKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLHIPKGLSIWIP 423

Query: 425 VLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKIILAML 484
           VLAIHHSEELWGKD NEF PERFASK F+ GRFIPFA GPRNCIGQ FA+MEAKIILAML
Sbjct: 424 VLAIHHSEELWGKDANEFNPERFASKSFTPGRFIPFATGPRNCIGQSFAMMEAKIILAML 483

Query: 485 LSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPIN 520
           +S+FSF+IS +YRHAPV VLTI+PKYGVQV +TPI+
Sbjct: 484 ISRFSFTISPNYRHAPVIVLTIKPKYGVQVCMTPID 515

BLAST of Cucsa.031370 vs. TrEMBL
Match: U5GG73_POPTR (Cytochrome P450 family protein OS=Populus trichocarpa GN=POPTR_0005s15710g PE=3 SV=1)

HSP 1 Score: 822.0 bits (2122), Expect = 4.1e-235
Identity = 394/519 (75.92%), Postives = 460/519 (88.63%), Query Frame = 1

Query: 3   IMIILTTLLVIFISSLAKIAYDTILCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDVAA 62
           I+++LTTLLVIF+ S  KIAYDT+ CY+LTPRRI+K MEKQGVRGP PRPL+GNILDVA 
Sbjct: 4   IVLVLTTLLVIFLISFFKIAYDTLSCYFLTPRRIKKIMEKQGVRGPKPRPLIGNILDVAD 63

Query: 63  LLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVLSK 122
            + +STAKDM  I H+ V+RLLPHY  W+KQYGKRFIYWNG+EPRLC+ ETELIKE+L+K
Sbjct: 64  FVSQSTAKDMDHITHDTVNRLLPHYVAWSKQYGKRFIYWNGVEPRLCITETELIKELLTK 123

Query: 123 NSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYVGLMVECTNK 182
            S+  G+SWLQR+GTKHFIG+GLLMANG DWYHQRHI AP+FMG++LKSY GLMVECT K
Sbjct: 124 YSSKAGKSWLQREGTKHFIGRGLLMANGSDWYHQRHIAAPAFMGERLKSYAGLMVECTKK 183

Query: 183 LIQSLETEFDSGRTEFEIADYMKQLTANIISRTEFDCNSDKGNQIFHLLTLLQHLCAQAS 242
           ++QSL+   +SG+TE EI +YM +++A+IISRTEFD + +KG QIFHLLT LQ LC QA+
Sbjct: 184 MLQSLQNAVESGQTEVEIGEYMTRVSADIISRTEFDSSYEKGKQIFHLLTELQSLCHQAT 243

Query: 243 RHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNE 302
           RHLCLPGSRFFPS YNR+IKS KMEV+RLL+EIIQSRKD VEIGRS+ YG+DLLG+L+NE
Sbjct: 244 RHLCLPGSRFFPSNYNRQIKSKKMEVDRLLLEIIQSRKDCVEIGRSSSYGNDLLGILVNE 303

Query: 303 MQRRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPKWQQKVRHEVN 362
           M+++R D   GF++NLQLIMDECKTFFFAGHETTALLLTWT+MLLA+NP WQ+KVR EVN
Sbjct: 304 MEKKRSD---GFNINLQLIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQEKVRAEVN 363

Query: 363 QVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWI 422
           +VC NG TPS++HLSKF LLNMVINESLRLYPPAT+LPRMAFEDIK+GDL +PKGL IWI
Sbjct: 364 EVC-NGETPSIDHLSKFNLLNMVINESLRLYPPATLLPRMAFEDIKLGDLHVPKGLQIWI 423

Query: 423 PVLAIHHSEELWGKDVNEFKPERFASKPFSGGR-FIPFAAGPRNCIGQGFALMEAKIILA 482
           PVLAIHHSEE+WGKD NEF P+RFASKPF+ GR FIPFA GPRNCIGQ FA+MEAKIILA
Sbjct: 424 PVLAIHHSEEIWGKDANEFNPDRFASKPFAPGRHFIPFATGPRNCIGQSFAMMEAKIILA 483

Query: 483 MLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINS 521
           ML+S+FSF ISDSYRHAPV VLTI+PKYGVQVYL P+NS
Sbjct: 484 MLISQFSFHISDSYRHAPVVVLTIKPKYGVQVYLKPLNS 518

BLAST of Cucsa.031370 vs. TrEMBL
Match: A0A067F681_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g010083mg PE=3 SV=1)

HSP 1 Score: 820.8 bits (2119), Expect = 9.1e-235
Identity = 396/522 (75.86%), Postives = 463/522 (88.70%), Query Frame = 1

Query: 1   MAIMIIL-TTLLVIFISSLAKIAYDTILCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILD 60
           MAI+++L +++ VIF+S L ++AYDT+ CYWLTPRRI+K M +QGVRGP PRPL GNIL+
Sbjct: 1   MAIVVLLISSVAVIFVSLLFRVAYDTLSCYWLTPRRIKKIMARQGVRGPNPRPLTGNILE 60

Query: 61  VAALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEV 120
           +AAL+ +ST+KD   I H+IV RLLPHY TW++QYGKRFIYWNG+EPR+CL+ET+LIKE+
Sbjct: 61  MAALISQSTSKDYDHIHHDIVGRLLPHYVTWSQQYGKRFIYWNGIEPRMCLSETDLIKEL 120

Query: 121 LSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYVGLMVEC 180
           L+K S   G+SWLQ+QG+KHFIGKGLLMANGEDWYHQRHIVAP+FMGD+LKSY G MVEC
Sbjct: 121 LTKFSTKAGKSWLQQQGSKHFIGKGLLMANGEDWYHQRHIVAPAFMGDRLKSYAGHMVEC 180

Query: 181 TNKLIQSLETEFDSGRTEFEIADYMKQLTANIISRTEFDCNSDKGNQIFHLLTLLQHLCA 240
           TNK+++SL+   +SG+ EFEI +YM +LTA+IISRTEFD + +KG QIFHLLT+LQHLCA
Sbjct: 181 TNKMLKSLQNAVESGQKEFEIGEYMTRLTADIISRTEFDSSYEKGQQIFHLLTVLQHLCA 240

Query: 241 QASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGML 300
           Q+SRHLC PGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKD VEIGRS+ YG+DLLGML
Sbjct: 241 QSSRHLCFPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDCVEIGRSSSYGNDLLGML 300

Query: 301 LNEMQRRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPKWQQKVRH 360
           LNEMQ +R    NGFSLN QLIMDECKTFFFAGHETTALLLTWT+MLLA+NP WQ+K+R 
Sbjct: 301 LNEMQNKR---GNGFSLNFQLIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQEKIRA 360

Query: 361 EVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLS 420
           EV QVC NG  PSV+HL K T LNMVINESLRLYPPATVLPRMAFEDIK+GDL IPKGLS
Sbjct: 361 EVKQVC-NGDAPSVDHLPKLTQLNMVINESLRLYPPATVLPRMAFEDIKLGDLLIPKGLS 420

Query: 421 IWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGR-FIPFAAGPRNCIGQGFALMEAKI 480
           IWIP+LAIHHSEELWGKD NEF P+RFAS+ F+ GR F PFAAGPRNC+GQ FA+MEAKI
Sbjct: 421 IWIPMLAIHHSEELWGKDANEFNPDRFASRTFASGRHFFPFAAGPRNCVGQSFAMMEAKI 480

Query: 481 ILAMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINS 521
           ILAML+S+F+F+ISDSYRHAPV VLTI+PKYGVQVYL P+++
Sbjct: 481 ILAMLISRFNFTISDSYRHAPVVVLTIKPKYGVQVYLEPVDT 518

BLAST of Cucsa.031370 vs. TAIR10
Match: AT5G38450.1 (AT5G38450.1 cytochrome P450, family 735, subfamily A, polypeptide 1)

HSP 1 Score: 730.7 bits (1885), Expect = 6.3e-211
Identity = 353/519 (68.02%), Postives = 427/519 (82.27%), Query Frame = 1

Query: 3   IMIILTTLLVIFISSLAKIAYDTILCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDVAA 62
           ++ IL +LLVIF++++ ++ YDTI CYWLTPRRI+K ME+QGV GP PRPL GNIL+++A
Sbjct: 2   LLTILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISA 61

Query: 63  LLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVLSK 122
           ++ +S +KD  SI H+IV RLLPHY  W+KQYGKRFI WNG +PRLCL ETELIKE+L K
Sbjct: 62  MVSQSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMK 121

Query: 123 NSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYVGLMVECTNK 182
           ++ V GRSWLQ+QGTK+FIG+GLLMANG+DW+HQRH+ AP+F G++LK Y   MVECT+K
Sbjct: 122 HNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSK 181

Query: 183 LIQSLETEFDSGRTEFEIADYMKQLTANIISRTEFDCNSDKGNQIFHLLTLLQHLCAQAS 242
           L++ L  E   G  E EI + M +LTA+IISRT+F  + +KG ++F+ LT+LQ  CAQA+
Sbjct: 182 LVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQAT 241

Query: 243 RHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNE 302
           RHLC PGSRF PSKYNREIKSLK EVERLL+EIIQSR+D  E+GRS+ +G DLLG+LLNE
Sbjct: 242 RHLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNE 301

Query: 303 MQRRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPKWQQKVRHEVN 362
           M   + + +N  + NLQLIMDECKTFFFAGHETTALLLTWT MLLA NP WQ+KVR EV 
Sbjct: 302 MDIDKNNNNN--NNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVR 361

Query: 363 QVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWI 422
           +V      PSV+ LSK T L+ VINESLRLYPPAT+LPRMAFED+K+GDL IPKGLSIWI
Sbjct: 362 EVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWI 421

Query: 423 PVLAIHHSEELWGKDVNEFKPERFASKPFSGGR-FIPFAAGPRNCIGQGFALMEAKIILA 482
           PVLAIHHSEELWGKD N+F PERF  +PF+ GR FIPFAAGPRNCIGQ FALMEAKIILA
Sbjct: 422 PVLAIHHSEELWGKDANQFNPERFGGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILA 481

Query: 483 MLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINS 521
            L+SKF+F+IS +YRHAP+ VLTI+PKYGVQV L P+ S
Sbjct: 482 TLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPLVS 518

BLAST of Cucsa.031370 vs. TAIR10
Match: AT1G67110.1 (AT1G67110.1 cytochrome P450, family 735, subfamily A, polypeptide 2)

HSP 1 Score: 706.8 bits (1823), Expect = 9.7e-204
Identity = 346/520 (66.54%), Postives = 415/520 (79.81%), Query Frame = 1

Query: 1   MAIMIILTTLLVIFISSLAKIAYDTILCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60
           M + ++L  +LVI ++ + ++ YD+I CY+LTPRRI+K ME+QG+ GP PR L GNI+D+
Sbjct: 1   MMVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDI 60

Query: 61  AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120
           + +L  S + D SSI HNIV RLLPHY +W+KQYGKRFI WNG EPRLCL ETE+IKE+L
Sbjct: 61  SKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELL 120

Query: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYVGLMVECT 180
           +K++ V G+SWLQ+QGTK FIG+GLLMANGE W+HQRH+ AP+F  D+LK Y   MVECT
Sbjct: 121 TKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECT 180

Query: 181 NKLIQSLETEFDSGRTEFEIADYMKQLTANIISRTEFDCNSDKGNQIFHLLTLLQHLCAQ 240
             + + L  E      E EI + M++LTA+IISRTEF  + DKG ++F LLT+LQ LCAQ
Sbjct: 181 KMMAERLRKEVGE---EVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300
           A+RHLC PGSRF PSKYNREIKSLK EVERLLMEII SRKD VEIGRS+ YG DLLG+LL
Sbjct: 241 ATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLL 300

Query: 301 NEMQRRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPKWQQKVRHE 360
           N+M        N  +LN+Q+IMDECKTFFF GHETT+LLLTWT+MLLA NP WQ  VR E
Sbjct: 301 NQMD------SNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDE 360

Query: 361 VNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSI 420
           V QVC     PSVE LS  T LN VINESLRLYPPAT+LPRMAFEDIK+GDL IPKGLSI
Sbjct: 361 VRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSI 420

Query: 421 WIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGR-FIPFAAGPRNCIGQGFALMEAKII 480
           WIPVLAIHHS ELWG+D NEF PERF ++ F+  R F+PFAAGPRNCIGQ FA+MEAKII
Sbjct: 421 WIPVLAIHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKII 480

Query: 481 LAMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPIN 520
           LAML+SKFSF+IS++YRHAP+ VLTI+PKYGVQ+ L P++
Sbjct: 481 LAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of Cucsa.031370 vs. TAIR10
Match: AT2G46950.1 (AT2G46950.1 cytochrome P450, family 709, subfamily B, polypeptide 2)

HSP 1 Score: 351.3 bits (900), Expect = 1.0e-96
Identity = 195/525 (37.14%), Postives = 292/525 (55.62%), Query Frame = 1

Query: 1   MAIMIILTTLLVIFISSLAKIAYDTILCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60
           M ++  +  L +  +  +    Y         P  + ++ +KQG+ GP  R L GN+ ++
Sbjct: 56  MELLSTINLLAIALVLLVVPKIYGACRILVWRPWMLSRRFKKQGISGPKYRILYGNLREI 115

Query: 61  AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120
             +  ++    +    ++IV R+LPH   W  QYG+ F+YW G +PRLC+++ EL K++L
Sbjct: 116 RKMKNEAKLMVLDPNSNDIVPRVLPHLQQWKSQYGETFLYWQGTDPRLCISDHELAKQIL 175

Query: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYVGLMVECT 180
           S       +S  + +  K   G GL+  NG DW   R I+ P+F  DKLK    LMV+CT
Sbjct: 176 SNKFVFFSKSKTKPEILK-LSGNGLIFVNGLDWVRHRRILNPAFSMDKLKLMTQLMVDCT 235

Query: 181 NKLIQSLETEFDSGRTE--FEIADYMKQLTANIISRTEFDCNSDKGNQIFHLLTLLQHLC 240
            ++    + + +   TE    I+   K+LTA+II+   F  +  +G ++F     LQ  C
Sbjct: 236 FRMFLEWKKQRNGVETEQFVLISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCC 295

Query: 241 AQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGM 300
           A A   L  PG ++ P+  N +I  L M+V   +  II +R        S  YG+DLLG+
Sbjct: 296 AAALTDLYFPGIQYLPTPSNLQIWKLDMKVNSSIKRIIDARLTS----ESKDYGNDLLGI 355

Query: 301 LLNEMQRRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPKWQQKVR 360
           +L        +      +++  I++ECKTFFFAGHETTA LLTW+ MLL+ +  WQ+K+R
Sbjct: 356 MLTAASSNESEK----KMSIDEIIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLR 415

Query: 361 HEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGL 420
            EV   C     P  E  SK  L+N V  ESLRLY P   L R+A ED+K+G+LEIPKG 
Sbjct: 416 EEVFNECGKDKIPDAETCSKLKLMNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKGT 475

Query: 421 SIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGR----FIPFAAGPRNCIGQGFALM 480
           +I +P+  +H  + +WG D ++F P RFA+            + F+ GPR CIGQ FA+M
Sbjct: 476 TIILPIAKMHRDKAVWGSDADKFNPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAIM 535

Query: 481 EAKIILAMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPIN 520
           EAK +LAM+L +F  ++S  Y+HAP   LT+QP+Y + V L PI+
Sbjct: 536 EAKTVLAMILQRFRLNLSADYKHAPADHLTLQPQYDLPVILEPID 571

BLAST of Cucsa.031370 vs. TAIR10
Match: AT2G46960.2 (AT2G46960.2 cytochrome P450, family 709, subfamily B, polypeptide 1)

HSP 1 Score: 350.1 bits (897), Expect = 2.3e-96
Identity = 191/528 (36.17%), Postives = 301/528 (57.01%), Query Frame = 1

Query: 1   MAIMIILTTLLVIFISSLAKIAYDTILCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60
           M ++I L  +++I I  L +I    ++  W  P  + ++++ QG+ GP  R   GN+ ++
Sbjct: 1   MGLVIFLALIVLILIIGL-RIFKAFMILVW-HPFVLTRRLKNQGISGPNYRIFYGNLSEI 60

Query: 61  AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120
             +  +S    +    ++I  R+LPHY  W  QYG+ F+YWNG EPR+C+++ EL K +L
Sbjct: 61  KKMKRESHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKTML 120

Query: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYVGLMVECT 180
           S       +S  + +  K    KGL+   G DW   R I+ P+F  D+LK    +MV+CT
Sbjct: 121 SNKLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVDCT 180

Query: 181 NKLIQSLETEFDSGRTEF-----EIADYMKQLTANIISRTEFDCNSDKGNQIFHLLTLLQ 240
            K+++    E     TE      E+ +  ++LTA+II+ + F  +  +G ++F     L+
Sbjct: 181 LKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMELK 240

Query: 241 HLCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDL 300
                +   + +PG+++ P+  N  +  L+ +++  +  II SR     +   + YG DL
Sbjct: 241 RCYTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSR-----LQSKSDYGDDL 300

Query: 301 LGMLLNEMQRRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPKWQQ 360
           LG+LL       ++      ++++ I+ EC+TFFF GHETT+ LL WT MLL+ +  WQ+
Sbjct: 301 LGILLKAYNTEGKER----KMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQE 360

Query: 361 KVRHEVNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIP 420
           K+R E+ + C    TP  E  SK  L+NMVI ESLRLY P + L R A  +IK+GDLEIP
Sbjct: 361 KLREEIFKECGKEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEIP 420

Query: 421 KGLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGR----FIPFAAGPRNCIGQGF 480
           KG ++ IP+L +H  + LWG D ++F P RFA+            + F+ GPR CIGQ F
Sbjct: 421 KGTTVVIPLLKMHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPRACIGQNF 480

Query: 481 ALMEAKIILAMLLSKFSF-SISDSYRHAPVSVLTIQPKYGVQVYLTPI 519
            ++EAK +L M+L +F F S+ D Y+H PV  +TIQP+YG+ V L P+
Sbjct: 481 VMIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPL 517

BLAST of Cucsa.031370 vs. TAIR10
Match: AT5G52400.1 (AT5G52400.1 cytochrome P450, family 715, subfamily A, polypeptide 1)

HSP 1 Score: 340.1 bits (871), Expect = 2.4e-93
Identity = 190/518 (36.68%), Postives = 283/518 (54.63%), Query Frame = 1

Query: 13  IFISSLAKIAYDTILCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDVAALLGKSTAKDM 72
           +F+  +  +     L  W+ P R +KK+   G  GP P    GN+ D+  L   S   D 
Sbjct: 16  VFVVVICLMFLKLFLRCWIWPVRAQKKLRGNGFVGPAPSFPFGNLNDMKKLKMASVVVDN 75

Query: 73  SS----IDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVLSKNSNVCG 132
           S     I+H+I    LPH+  W ++YGK F+YW G+EP + +A+ E +  V+SK   V G
Sbjct: 76  SKSSTIINHDIHSIALPHFARWQQEYGKVFVYWLGIEPFVYVADPEFLS-VMSKG--VLG 135

Query: 133 RSW----LQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYVGLMVECTNKLI 192
           +SW    + ++  +   G GL+M  G+DW   RHI+ P+F    LK    +MVE  + ++
Sbjct: 136 KSWGKPNVFKKDREPMFGTGLVMVEGDDWTRHRHIITPAFAPLNLKVMTNMMVESVSNML 195

Query: 193 QSLETEFDSGRTEFEIADYMKQLTANIISRTEFDCNSDKGNQIFHLLTLLQHLCAQASRH 252
                + +SG  EF++   +      II++T F    + G Q+   L  +Q     ++R+
Sbjct: 196 DRWGIQINSGNPEFDMESEIIGTAGEIIAKTSFGVTGENGTQVLKNLRAVQFALFNSNRY 255

Query: 253 LCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQ 312
           + +P S     K   + K L  E++ LL+  I  RK  + +      G DLLGMLL   Q
Sbjct: 256 VGVPFSNILSYKQTVKAKGLGHEIDGLLLSFINKRK--ISLAEGDDQGHDLLGMLLKADQ 315

Query: 313 RRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPKWQQKVRHEVNQV 372
           +         +   + ++DECKTFFFAGHETTAL LTWT MLLA +P+WQ  +R E+ +V
Sbjct: 316 KG--------NFTAKELVDECKTFFFAGHETTALALTWTFMLLAIHPEWQDTIREEIREV 375

Query: 373 CHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPV 432
             +        L+    ++ V+NE LRLYPPA    R A  DI+V    IP G +IWI V
Sbjct: 376 IGDSKI-EYNKLAGLKKMSWVMNEVLRLYPPAPNAQRQARNDIEVNGRVIPNGTNIWIDV 435

Query: 433 LAIHHSEELWGKDVNEFKPERFASKPFSGGR----FIPFAAGPRNCIGQGFALMEAKIIL 492
           +A+HH  ELWG DVNEFKPERF      G +    ++PF  G R CIG+    ME KI+L
Sbjct: 436 VAMHHDVELWGDDVNEFKPERFDGNLHGGCKNKMGYMPFGFGGRMCIGRNLTTMEYKIVL 495

Query: 493 AMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPI 519
           +++LS+F  S+S  YRH+P  +L+++P YG+ + + P+
Sbjct: 496 SLVLSRFEISVSPGYRHSPTYMLSLRPGYGLPLIIRPL 519

BLAST of Cucsa.031370 vs. NCBI nr
Match: gi|449455844|ref|XP_004145660.1| (PREDICTED: cytokinin hydroxylase-like [Cucumis sativus])

HSP 1 Score: 1053.1 bits (2722), Expect = 1.6e-304
Identity = 518/523 (99.04%), Postives = 520/523 (99.43%), Query Frame = 1

Query: 1   MAIMIILTTLLVIFISSLAKIAYDTILCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60
           MAIMIILTTLLVIFISSLAKIAYDTI CYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV
Sbjct: 1   MAIMIILTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61  AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120
           AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL
Sbjct: 61  AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120

Query: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYVGLMVECT 180
           SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSY GLMVECT
Sbjct: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLMVECT 180

Query: 181 NKLIQSLETEFDSGRTEFEIADYMKQLTANIISRTEFDCNSDKGNQIFHLLTLLQHLCAQ 240
           NKLIQSLETEFDSGRTEFEIADYMKQLTA+IISRTEFDCNSDKGNQIFHLLTLLQHLCAQ
Sbjct: 181 NKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDCNSDKGNQIFHLLTLLQHLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300
           ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL
Sbjct: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300

Query: 301 NEMQRRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPKWQQKVRHE 360
           NEMQ+RREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNP WQQKVRHE
Sbjct: 301 NEMQKRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHE 360

Query: 361 VNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSI 420
           VNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSI
Sbjct: 361 VNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSI 420

Query: 421 WIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKIIL 480
           WIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKIIL
Sbjct: 421 WIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKIIL 480

Query: 481 AMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINSYQP 524
           AMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINSYQP
Sbjct: 481 AMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINSYQP 523

BLAST of Cucsa.031370 vs. NCBI nr
Match: gi|659123291|ref|XP_008461587.1| (PREDICTED: cytokinin hydroxylase-like [Cucumis melo])

HSP 1 Score: 1036.2 bits (2678), Expect = 2.0e-299
Identity = 508/523 (97.13%), Postives = 516/523 (98.66%), Query Frame = 1

Query: 1   MAIMIILTTLLVIFISSLAKIAYDTILCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60
           MAI+I LTTLLVIFISSLAKIAYDTI CYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV
Sbjct: 1   MAIIIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61  AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120
           AALL KSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL
Sbjct: 61  AALLAKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120

Query: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYVGLMVECT 180
           SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSY GLMVECT
Sbjct: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLMVECT 180

Query: 181 NKLIQSLETEFDSGRTEFEIADYMKQLTANIISRTEFDCNSDKGNQIFHLLTLLQHLCAQ 240
           +KLIQSLETE DSGRTEFEIADYMKQLTA+IISRTEFD NSDKGNQIFHLLTLLQHLCAQ
Sbjct: 181 DKLIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQHLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300
           ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL
Sbjct: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300

Query: 301 NEMQRRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPKWQQKVRHE 360
           NEMQR+++DGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNP WQQKVRHE
Sbjct: 301 NEMQRKKDDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHE 360

Query: 361 VNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSI 420
           VNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSI
Sbjct: 361 VNQVCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSI 420

Query: 421 WIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKIIL 480
           WIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNC+GQGFALMEAKIIL
Sbjct: 421 WIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCVGQGFALMEAKIIL 480

Query: 481 AMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINSYQP 524
           AMLLSKFSF+ISDSYRHAPVSVLTIQPKYGVQVYLTPINSYQP
Sbjct: 481 AMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINSYQP 523

BLAST of Cucsa.031370 vs. NCBI nr
Match: gi|657975387|ref|XP_008379543.1| (PREDICTED: cytokinin hydroxylase-like [Malus domestica])

HSP 1 Score: 833.9 bits (2153), Expect = 1.5e-238
Identity = 407/516 (78.88%), Postives = 462/516 (89.53%), Query Frame = 1

Query: 5   IILTTLLVIFISSLAKIAYDTILCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDVAALL 64
           ++LTTLLV+F+S L ++AYDT+ CY+LTPRRI++ MEKQGVRGP PRPL GNILD+A L+
Sbjct: 4   VLLTTLLVLFLSLLLRVAYDTLSCYFLTPRRIKRIMEKQGVRGPKPRPLNGNILDMATLV 63

Query: 65  GKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVLSKNS 124
            KST+ DM + DH+IV RLLPHY  W+KQYGKRFIYWNG+EPR+CL+ETELIKE+LSK S
Sbjct: 64  TKSTSHDMHTNDHDIVGRLLPHYVAWSKQYGKRFIYWNGIEPRMCLSETELIKELLSKYS 123

Query: 125 NVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYVGLMVECTNKLI 184
            + G+SWLQ+QG+KHFIG+GLLMANGEDWYHQRHIVAP+F GD+LKSY G MVECT ++I
Sbjct: 124 TISGKSWLQQQGSKHFIGRGLLMANGEDWYHQRHIVAPAFTGDRLKSYAGYMVECTKQMI 183

Query: 185 QSLETEFD-SGRTEFEIADYMKQLTANIISRTEFDCNSDKGNQIFHLLTLLQHLCAQASR 244
           QSL+ E + SGR EFEI +YM +LTA+IISRTEFD + +KG QIF LLT+LQHLCAQASR
Sbjct: 184 QSLKNEIETSGRREFEIGEYMTRLTADIISRTEFDSSYEKGKQIFRLLTVLQHLCAQASR 243

Query: 245 HLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEM 304
           HLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKD VEIGRS+ YG+DLLGMLLNEM
Sbjct: 244 HLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDCVEIGRSSSYGNDLLGMLLNEM 303

Query: 305 QRRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPKWQQKVRHEVNQ 364
           Q++R    NGFSLNLQLIMDECKTFFFAGHETTALLLTWT+MLLA+NP WQ+KVR EV Q
Sbjct: 304 QKKR---GNGFSLNLQLIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQEKVRAEVKQ 363

Query: 365 VCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIP 424
           VC NG TPSV+HLSK TLLN VINESLRLYPPATVLPRMAFEDIK+GDL IPKGLSIWIP
Sbjct: 364 VC-NGGTPSVDHLSKLTLLNRVINESLRLYPPATVLPRMAFEDIKLGDLHIPKGLSIWIP 423

Query: 425 VLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKIILAML 484
           VLAIHHSEELWGKD NEF PERFASK F+ GRFIPFA GPRNCIGQ FA+MEAKIILAML
Sbjct: 424 VLAIHHSEELWGKDANEFNPERFASKSFTPGRFIPFATGPRNCIGQSFAMMEAKIILAML 483

Query: 485 LSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPIN 520
           +S+FSF++S +YRHAPV VLTI+PKYGVQV LTPI+
Sbjct: 484 ISQFSFTVSPNYRHAPVIVLTIKPKYGVQVCLTPID 515

BLAST of Cucsa.031370 vs. NCBI nr
Match: gi|694332889|ref|XP_009357060.1| (PREDICTED: cytokinin hydroxylase-like [Pyrus x bretschneideri])

HSP 1 Score: 833.2 bits (2151), Expect = 2.6e-238
Identity = 405/516 (78.49%), Postives = 461/516 (89.34%), Query Frame = 1

Query: 5   IILTTLLVIFISSLAKIAYDTILCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDVAALL 64
           ++LTTLLVIF+  L ++AYDT+ CY+LTPRRI++ MEKQGVRGP PRPL GNILD+A L+
Sbjct: 4   VLLTTLLVIFLGLLLRVAYDTLSCYFLTPRRIKRTMEKQGVRGPKPRPLNGNILDMATLV 63

Query: 65  GKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVLSKNS 124
            KST+ DM +IDH+IV RLLPHY  W+KQYGKRFIYWNG+EPR+CL+ETELIKE+LSK S
Sbjct: 64  AKSTSHDMHTIDHDIVGRLLPHYVAWSKQYGKRFIYWNGIEPRMCLSETELIKELLSKYS 123

Query: 125 NVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYVGLMVECTNKLI 184
            + G+SWLQ+QG+KHFIG+GLLMANGEDWYHQRHIVAP+F GD+LKSY G MVECT +++
Sbjct: 124 TISGKSWLQQQGSKHFIGRGLLMANGEDWYHQRHIVAPAFTGDRLKSYAGYMVECTKEML 183

Query: 185 QSLETEF-DSGRTEFEIADYMKQLTANIISRTEFDCNSDKGNQIFHLLTLLQHLCAQASR 244
           QSL+ E   SG+ EFEI +YM +LTA+IISRTEFD + +KG QIF LLT+LQHLCAQASR
Sbjct: 184 QSLKNEIGTSGKREFEIGEYMTRLTADIISRTEFDSSYEKGKQIFRLLTVLQHLCAQASR 243

Query: 245 HLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEM 304
           HLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSR+D VEIGRS+ YG+DLLGMLLNEM
Sbjct: 244 HLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRRDCVEIGRSSSYGNDLLGMLLNEM 303

Query: 305 QRRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPKWQQKVRHEVNQ 364
           Q++R    NGFSLNLQLIMDECKTFFFAGHETTALLLTWT+MLLA+NP WQ+KVR EV Q
Sbjct: 304 QKKR---GNGFSLNLQLIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQEKVRAEVKQ 363

Query: 365 VCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIP 424
           VC NG TPSV+HLSK TLLN VINESLRLYPPATVLPRMAFEDIK+GDL IPKGLSIWIP
Sbjct: 364 VC-NGGTPSVDHLSKLTLLNRVINESLRLYPPATVLPRMAFEDIKLGDLHIPKGLSIWIP 423

Query: 425 VLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKIILAML 484
           VLAIHHSEELWGKD NEF PERFASK F+ GRFIPFA GPRNCIGQ FA+MEAKIILAML
Sbjct: 424 VLAIHHSEELWGKDANEFNPERFASKSFTPGRFIPFATGPRNCIGQSFAMMEAKIILAML 483

Query: 485 LSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPIN 520
           +S+FSF++S +YRHAPV VLTI+PKYGVQV LTPI+
Sbjct: 484 ISQFSFTVSPNYRHAPVIVLTIKPKYGVQVCLTPID 515

BLAST of Cucsa.031370 vs. NCBI nr
Match: gi|255551354|ref|XP_002516723.1| (PREDICTED: cytokinin hydroxylase [Ricinus communis])

HSP 1 Score: 830.5 bits (2144), Expect = 1.7e-237
Identity = 407/518 (78.57%), Postives = 459/518 (88.61%), Query Frame = 1

Query: 4   MIILTTLLVIFISSLAKIAYDTILCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDVAAL 63
           M +LTT+LVI  S   K+AYDTI CY LTPRRI+K MEKQGVRGP PRPL GNILDV AL
Sbjct: 1   MAVLTTVLVIVFSLFFKVAYDTISCYLLTPRRIKKIMEKQGVRGPQPRPLTGNILDVTAL 60

Query: 64  LGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVLSKN 123
           + +ST++D   + H+IV RLLPH+ TW+KQYGKRFIYWNG+EPR+CL ETELIKE+L+K 
Sbjct: 61  VSQSTSRDCDYVHHDIVYRLLPHFVTWSKQYGKRFIYWNGIEPRMCLTETELIKELLTKY 120

Query: 124 SNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYVGLMVECTNKL 183
           S   G+SWLQ+QG+KHFIG+GLLMANG++WYHQRHIVAP+FMGD+LKSY G MVECT K+
Sbjct: 121 STKAGKSWLQQQGSKHFIGRGLLMANGDNWYHQRHIVAPAFMGDRLKSYSGHMVECTKKM 180

Query: 184 IQSLETEFDSGRTEFEIADYMKQLTANIISRTEFDCNSDKGNQIFHLLTLLQHLCAQASR 243
           +QSL+   +SG+TEFEI +YM +LTA+IISRTEFDC+ +KG QIFHLL +LQ LCAQASR
Sbjct: 181 LQSLQKAVESGQTEFEIGEYMTRLTADIISRTEFDCSYEKGKQIFHLLNVLQRLCAQASR 240

Query: 244 HLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEM 303
           HLC PGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKD VEIGRS+ YG+DLLGMLLNEM
Sbjct: 241 HLCFPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDCVEIGRSSTYGNDLLGMLLNEM 300

Query: 304 QRRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPKWQQKVRHEVNQ 363
           Q +RE   NGFSLNLQLIMDECKTFFFAGHETTALLLTWT+MLLA+NP WQ+KVR EVNQ
Sbjct: 301 QSKRE---NGFSLNLQLIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQEKVRAEVNQ 360

Query: 364 VCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIP 423
           VC NG TPSV+HL K TLLNMVINESLRLYPPATVLPRMAFEDIK+GDL IPKGLSIWIP
Sbjct: 361 VC-NGETPSVDHLPKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLHIPKGLSIWIP 420

Query: 424 VLAIHHSEELWGKDVNEFKPERFASKPFSGGR-FIPFAAGPRNCIGQGFALMEAKIILAM 483
           VLAIHHSEELWGKD NEF P+RFASK F+ GR FIPFAAGPRNCIGQ FA+MEAKIILAM
Sbjct: 421 VLAIHHSEELWGKDANEFNPDRFASKTFAPGRHFIPFAAGPRNCIGQTFAMMEAKIILAM 480

Query: 484 LLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINS 521
           L+SKF+F+ISDSYRHAPV VLTI+PKYGVQV L P+N+
Sbjct: 481 LISKFNFNISDSYRHAPVIVLTIKPKYGVQVCLRPLNN 514