Carg22272 (gene) Silver-seed gourd

NameCarg22272
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionCytochrome P450, putative
LocationCucurbita_argyrosperma_scaffold_164 : 113652 .. 115846 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGACCGAGTTGGAATCGGGCCGGACCGAGTTCGAGATCGCCGATTACATGAAGCAACTCACCGCCGACATCATTTCCCGGACCGAGTTCGACACCGACTCCGACAAGGGTAACCAAATCTTCCACTTACTCACTCTTCTCCAGCGCCTCTGCGCCCAAGCCAGCCGCCATCTCTGCCTTCCCGGTAGCCGGTAAGTTGACTGTTTTTTTTTAAATTTACAATTTTTCTATTTATGTATTATCTAACACTTAAAAACAAAAAAGAATTATATTAAAAAATTAAAAGCAAAATAGCTTTTACTGCGCCCAAGTCAACTGCTACCAATTATTGTCTTGTTTATGCTTTGACTTTTCGACTTTCCTTCAATGTTTTTAAAACGCGTGTATTACGGAGAACTTTATCCACTTTTGTAAAAAATGTTTTTCTCTCCTCTCCAACCGACATAGAATCTCACAATCCATCCCTTCAGGGCACAGCCTCCTGACTAACATTCATTCTTTTGTCCAATTGACGTGAGTTCTAACAATCTACTCAATCCATCCCCCTTCAGAGTCCAACCTCCTCGCTGACATTCATTCTTCTGTTCAATCGGTGTGGATTCTCACAATCTACTTCCTTCAGGGCCAGCATCATCGCTGGCATTCGTTCGCCTCTCCAATCAATGTGGATTCTCACAATTCACTCTCTTCGAGGCCAGTGTCCTTACTAGTACATCACCCAGTGTCTGATTAACCTTGAGGGAAGGTCCAAAGAAGACAATAATATGTACTAGCAGTGGACTTGGACCATTACATATGTTGTCCTTGTCCTTAACAAGTGGCATTAAAACCATGTCTCTAACTTAGCTATACTGATAGAATCTTTTAGGGTGGAATAAATTTGTTCGATGTTTTGGTGCAGGTTTGTTCCAAGTAAATACAACAGAGAAATAAAGTCACTAAAGAAGGAAGTGGAGAGGCTGTTAATGGAGATCATTGAAAGCAGAAAAGATGGTGTGGAGATAGGAAGAAGCAGTTGCTATGGAAGTGATCTATTGGGAATGCTATTGAATGAGATGCAACAGAGGAAGAACATCAGCACCACCACCACCACCCTCAACTTGCAGCTCATAATGGACGAATGCAAGACCTTCTTCTTTGCTGGCCATGAAACCACCGCTCTTTTGCTCACTTGGACCATCATGTTGCTTGCTACTAACCCTAATTGGCAACACATGGTTCGTGCCCAAGTTAACCAAGTTTGCAACGCTCGTACCCCGTCTATTCAACATCTCTCTAAGCTCACTTTGGTAACCAATCTATCTCCTACTCGTTCCATCAAAATTTTCAAAAATACCCTTAAATTTTTATAACAAAATCATTTCAAAACCACCCACCAATTTCATATTAACATTCTTAACTGTCCTGTTAAAACAATTTAAAAAAGTCCTTACAGATGGAAATCCTTTGTCTACCTCTCGTAGATCATCTCCCTCTTTAATTTCAAGGGTATTATCAGTAAAAATATTTTTAATTTTTTTTTCTAGAAATTCATGGGTATTAATGTTACTTTTGAAACATGGTACTAACAGTTTCGTCTATACACTAATGATAAAGGTATTTGTGATCTTTCCACTGACATGACAAATTGTAAGTTTAGTATTATTAAAACTTTTAAAAGTTCGAGACATTCCGCTTTAAATTTAGCAATGAATTAAGTAGAGTATGTGTGTAGTACGAGGAACATGAGACATGACATTGATGCACACATGATATGTTTTGGTTTTGGTCAGCTAAACATGGTGATAAACGAGTCCCTAAGGCTTTACCCGCCCGCCACAGTGCTTCCAAGAATGGCGTTGGAGGACATGAAGCTAGGAGACATGGACATCCCCAAGGGCCTGTCCATATGGATTCCGGTGCTGGCGATTCACCACAGCGAAGAGCTGTGGGGCAAAGATGCCAACCAGTTCAACCCCAACAGGTTCGGCGCCAAGGCGTTCGGTGGCGGGCGGTTCATTCCATTTGCAGGCGGACCAAGAAACTGCATCGGCCAAGGGTTCGCCATCATGGAAGCTAAGATCATCTTGGCTATGTTCCTCTCCAAGTTTAGGTTCACTATTTCCGACACTTATCGCCATGCTCCTGTTGTTGTTCTCACCATTCAGCCTAAGTATGGAGTTCAAGTTTGTTTGACTCCCTTCACTTCTTAG

mRNA sequence

ATGGAGACCGAGTTGGAATCGGGCCGGACCGAGTTCGAGATCGCCGATTACATGAAGCAACTCACCGCCGACATCATTTCCCGGACCGAGTTCGACACCGACTCCGACAAGGGTAACCAAATCTTCCACTTACTCACTCTTCTCCAGCGCCTCTGCGCCCAAGCCAGCCGCCATCTCTGCCTTCCCGGTAGCCGGTTTGTTCCAAGTAAATACAACAGAGAAATAAAGTCACTAAAGAAGGAAGTGGAGAGGCTGTTAATGGAGATCATTGAAAGCAGAAAAGATGGTGTGGAGATAGGAAGAAGCAGTTGCTATGGAAGTGATCTATTGGGAATGCTATTGAATGAGATGCAACAGAGGAAGAACATCAGCACCACCACCACCACCCTCAACTTGCAGCTCATAATGGACGAATGCAAGACCTTCTTCTTTGCTGGCCATGAAACCACCGCTCTTTTGCTCACTTGGACCATCATGTTGCTTGCTACTAACCCTAATTGGCAACACATGGTTCGTGCCCAAGTTAACCAAGTTTGCAACGCTCGTACCCCGTCTATTCAACATCTCTCTAAGCTCACTTTGCTAAACATGGTGATAAACGAGTCCCTAAGGCTTTACCCGCCCGCCACAGTGCTTCCAAGAATGGCGTTGGAGGACATGAAGCTAGGAGACATGGACATCCCCAAGGGCCTGTCCATATGGATTCCGGTGCTGGCGATTCACCACAGCGAAGAGCTGTGGGGCAAAGATGCCAACCAGTTCAACCCCAACAGGTTCGGCGCCAAGGCGTTCGGTGGCGGGCGGTTCATTCCATTTGCAGGCGGACCAAGAAACTGCATCGGCCAAGGGTTCGCCATCATGGAAGCTAAGATCATCTTGGCTATGTTCCTCTCCAAGTTTAGGTTCACTATTTCCGACACTTATCGCCATGCTCCTGTTGTTGTTCTCACCATTCAGCCTAAGTATGGAGTTCAAGTTTGTTTGACTCCCTTCACTTCTTAG

Coding sequence (CDS)

ATGGAGACCGAGTTGGAATCGGGCCGGACCGAGTTCGAGATCGCCGATTACATGAAGCAACTCACCGCCGACATCATTTCCCGGACCGAGTTCGACACCGACTCCGACAAGGGTAACCAAATCTTCCACTTACTCACTCTTCTCCAGCGCCTCTGCGCCCAAGCCAGCCGCCATCTCTGCCTTCCCGGTAGCCGGTTTGTTCCAAGTAAATACAACAGAGAAATAAAGTCACTAAAGAAGGAAGTGGAGAGGCTGTTAATGGAGATCATTGAAAGCAGAAAAGATGGTGTGGAGATAGGAAGAAGCAGTTGCTATGGAAGTGATCTATTGGGAATGCTATTGAATGAGATGCAACAGAGGAAGAACATCAGCACCACCACCACCACCCTCAACTTGCAGCTCATAATGGACGAATGCAAGACCTTCTTCTTTGCTGGCCATGAAACCACCGCTCTTTTGCTCACTTGGACCATCATGTTGCTTGCTACTAACCCTAATTGGCAACACATGGTTCGTGCCCAAGTTAACCAAGTTTGCAACGCTCGTACCCCGTCTATTCAACATCTCTCTAAGCTCACTTTGCTAAACATGGTGATAAACGAGTCCCTAAGGCTTTACCCGCCCGCCACAGTGCTTCCAAGAATGGCGTTGGAGGACATGAAGCTAGGAGACATGGACATCCCCAAGGGCCTGTCCATATGGATTCCGGTGCTGGCGATTCACCACAGCGAAGAGCTGTGGGGCAAAGATGCCAACCAGTTCAACCCCAACAGGTTCGGCGCCAAGGCGTTCGGTGGCGGGCGGTTCATTCCATTTGCAGGCGGACCAAGAAACTGCATCGGCCAAGGGTTCGCCATCATGGAAGCTAAGATCATCTTGGCTATGTTCCTCTCCAAGTTTAGGTTCACTATTTCCGACACTTATCGCCATGCTCCTGTTGTTGTTCTCACCATTCAGCCTAAGTATGGAGTTCAAGTTTGTTTGACTCCCTTCACTTCTTAG

Protein sequence

METELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLCLPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQRKNISTTTTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCNARTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAIHHSEELWGKDANQFNPNRFGAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSKFRFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS
BLAST of Carg22272 vs. NCBI nr
Match: XP_022934803.1 (cytokinin hydroxylase [Cucurbita moschata])

HSP 1 Score: 654.4 bits (1687), Expect = 2.0e-184
Identity = 329/333 (98.80%), Postives = 329/333 (98.80%), Query Frame = 0

Query: 1   METELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLC 60
           METELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLC
Sbjct: 187 METELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLC 246

Query: 61  LPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQR 120
           LPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQR
Sbjct: 247 LPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQR 306

Query: 121 KNISTTTTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCN 180
           KNIS   TTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCN
Sbjct: 307 KNIS---TTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCN 366

Query: 181 ARTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAI 240
           AR PSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAI
Sbjct: 367 ARAPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAI 426

Query: 241 HHSEELWGKDANQFNPNRFGAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSKF 300
           HHSEELWGKDANQFNPNRFGAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSKF
Sbjct: 427 HHSEELWGKDANQFNPNRFGAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSKF 486

Query: 301 RFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS 334
           RFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS
Sbjct: 487 RFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS 516

BLAST of Carg22272 vs. NCBI nr
Match: XP_022983651.1 (cytokinin hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 645.6 bits (1664), Expect = 9.3e-182
Identity = 323/333 (97.00%), Postives = 329/333 (98.80%), Query Frame = 0

Query: 1   METELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLC 60
           METEL+SGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLC
Sbjct: 187 METELDSGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLC 246

Query: 61  LPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQR 120
           LPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQR
Sbjct: 247 LPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQR 306

Query: 121 KNISTTTTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCN 180
           KN  +TTTTLNLQLI+DECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRA+VNQVCN
Sbjct: 307 KN--STTTTLNLQLIIDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAEVNQVCN 366

Query: 181 ARTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAI 240
           ARTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAI
Sbjct: 367 ARTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAI 426

Query: 241 HHSEELWGKDANQFNPNRFGAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSKF 300
           HHSEELWGKDANQFNPNRF AKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSKF
Sbjct: 427 HHSEELWGKDANQFNPNRFAAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSKF 486

Query: 301 RFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS 334
            FTISDTYRHAPVVVLTIQPK+GVQVCLTPF+S
Sbjct: 487 SFTISDTYRHAPVVVLTIQPKHGVQVCLTPFSS 517

BLAST of Carg22272 vs. NCBI nr
Match: XP_023528555.1 (cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 636.3 bits (1640), Expect = 5.7e-179
Identity = 321/334 (96.11%), Postives = 322/334 (96.41%), Query Frame = 0

Query: 1   METELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLC 60
           METELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLC
Sbjct: 187 METELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLC 246

Query: 61  LPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQ- 120
           LPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNE+QQ 
Sbjct: 247 LPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNELQQX 306

Query: 121 RKNISTTTTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVC 180
                     LNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVC
Sbjct: 307 XXXXXXXXXXLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVC 366

Query: 181 NARTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLA 240
           NARTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLA
Sbjct: 367 NARTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLA 426

Query: 241 IHHSEELWGKDANQFNPNRFGAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSK 300
           IHHSEELWGKDANQFNPNRFGAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAM LSK
Sbjct: 427 IHHSEELWGKDANQFNPNRFGAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMLLSK 486

Query: 301 FRFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS 334
           FRFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS
Sbjct: 487 FRFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS 520

BLAST of Carg22272 vs. NCBI nr
Match: XP_008461587.1 (PREDICTED: cytokinin hydroxylase-like [Cucumis melo])

HSP 1 Score: 569.7 bits (1467), Expect = 6.5e-159
Identity = 282/334 (84.43%), Postives = 303/334 (90.72%), Query Frame = 0

Query: 1   METELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLC 60
           +ETEL+SGRTEFEIADYMKQLTADIISRTEFD +SDKGNQIFHLLTLLQ LCAQASRHLC
Sbjct: 187 LETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQHLCAQASRHLC 246

Query: 61  LPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQR 120
           LPGSRF PSKYNREIKSLK EVERLLMEII+SRKDGVEIGRS+CYGSDLLGMLLNEMQ++
Sbjct: 247 LPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRK 306

Query: 121 KNISTTTTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVC- 180
           K+      +LNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQ  VR +VNQVC 
Sbjct: 307 KDDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHEVNQVCH 366

Query: 181 NARTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLA 240
           N  TPS++HLSK TLLNMVINESLRLYPPATVLPRMA ED+K+GD++IPKGLSIWIPVLA
Sbjct: 367 NGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLA 426

Query: 241 IHHSEELWGKDANQFNPNRFGAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSK 300
           IHHSEELWGKD N+F P RF +K F GGRFIPFA GPRNC+GQGFA+MEAKIILAM LSK
Sbjct: 427 IHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCVGQGFALMEAKIILAMLLSK 486

Query: 301 FRFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS 334
           F FTISD+YRHAPV VLTIQPKYGVQV LTP  S
Sbjct: 487 FSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 520

BLAST of Carg22272 vs. NCBI nr
Match: XP_022148131.1 (cytokinin hydroxylase-like [Momordica charantia])

HSP 1 Score: 568.9 bits (1465), Expect = 1.1e-158
Identity = 282/333 (84.68%), Postives = 303/333 (90.99%), Query Frame = 0

Query: 1   METELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLC 60
           ++TEL SGRTEFEIA+YMKQLTADIISRTEFD++ DKG QIFHLLTLLQRLCAQASRHLC
Sbjct: 98  LQTELNSGRTEFEIAEYMKQLTADIISRTEFDSNYDKGKQIFHLLTLLQRLCAQASRHLC 157

Query: 61  LPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQR 120
           LPGSRF PS+YNREIKSLK EVERLLMEIIESRKDGVEIGRSSCYG+DLLGMLLNEMQ+ 
Sbjct: 158 LPGSRFFPSQYNREIKSLKMEVERLLMEIIESRKDGVEIGRSSCYGNDLLGMLLNEMQRP 217

Query: 121 KNISTTTTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCN 180
           K       +LNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQH +RAQ+NQVCN
Sbjct: 218 KR--EDGLSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKLRAQINQVCN 277

Query: 181 ARTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAI 240
             TP++  LS+LTLLNMVINESLRLYPPATVLPRMA ED+KLGD++IPKGLSIWIPVLAI
Sbjct: 278 GATPTLDDLSQLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAI 337

Query: 241 HHSEELWGKDANQFNPNRFGAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSKF 300
           HHSEELWGKDAN+FNP RF  K+   GRFIPFA GPRNCIGQ FA+MEAKIILAMFLSKF
Sbjct: 338 HHSEELWGKDANEFNPERFALKSSSAGRFIPFAAGPRNCIGQAFAMMEAKIILAMFLSKF 397

Query: 301 RFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS 334
            FTISDTYRHAPV+VLTIQPKYGVQVCLTP  S
Sbjct: 398 SFTISDTYRHAPVIVLTIQPKYGVQVCLTPLNS 428

BLAST of Carg22272 vs. TAIR10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2)

HSP 1 Score: 462.2 bits (1188), Expect = 2.7e-130
Identity = 232/322 (72.05%), Postives = 265/322 (82.30%), Query Frame = 0

Query: 11  EFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLCLPGSRFVPSK 70
           E EI + M++LTADIISRTEF +  DKG ++F LLT+LQRLCAQA+RHLC PGSRF+PSK
Sbjct: 194 EVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQATRHLCFPGSRFLPSK 253

Query: 71  YNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQRKNISTTTTTL 130
           YNREIKSLK EVERLLMEII+SRKD VEIGRSS YG DLLG+LLN+M   KN       L
Sbjct: 254 YNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMDSNKN------NL 313

Query: 131 NLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCNA-RTPSIQHL 190
           N+Q+IMDECKTFFF GHETT+LLLTWT+MLLA NP WQ  VR +V QVC     PS++ L
Sbjct: 314 NVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGVPSVEQL 373

Query: 191 SKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAIHHSEELWGK 250
           S LT LN VINESLRLYPPAT+LPRMA ED+KLGD+ IPKGLSIWIPVLAIHHS ELWG+
Sbjct: 374 SSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHHSNELWGE 433

Query: 251 DANQFNPNRFGAKAFGGGR-FIPFAGGPRNCIGQGFAIMEAKIILAMFLSKFRFTISDTY 310
           DAN+FNP RF  ++F   R F+PFA GPRNCIGQ FA+MEAKIILAM +SKF F IS+ Y
Sbjct: 434 DANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKIILAMLVSKFSFAISENY 493

Query: 311 RHAPVVVLTIQPKYGVQVCLTP 331
           RHAP+VVLTI+PKYGVQ+ L P
Sbjct: 494 RHAPIVVLTIKPKYGVQLVLKP 509

BLAST of Carg22272 vs. TAIR10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1)

HSP 1 Score: 389.8 bits (1000), Expect = 1.7e-108
Identity = 207/335 (61.79%), Postives = 239/335 (71.34%), Query Frame = 0

Query: 1   METELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLC 60
           +  E+  G  E EI + M +LTADIISRT+F +  +KG ++F+ LT+LQR CAQA+RHLC
Sbjct: 186 LRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQATRHLC 245

Query: 61  LPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQR 120
            PGSRF+PSKYNREIKSLKKEVERLL+EII+SR+D  E+GRSS +G DLLG+LLNEM   
Sbjct: 246 FPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMD-- 305

Query: 121 KNISTTTTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCN 180
            +                                    MLLA NP WQ  VR +V +V  
Sbjct: 306 IDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXMLLADNPTWQEKVREEVREVFG 365

Query: 181 AR-TPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLA 240
               PS+  LSKLT L+ VINESLRLYPPAT+LPRMA ED+KLGD+ IPKGLSIWIPVLA
Sbjct: 366 RNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLA 425

Query: 241 IHHSEELWGKDANQFNPNRFGAKAFGGGR-FIPFAGGPRNCIGQGFAIMEAKIILAMFLS 300
           IHHSEELWGKDANQFNP RFG + F  GR FIPFA GPRNCIGQ FA+MEAKIILA  +S
Sbjct: 426 IHHSEELWGKDANQFNPERFGGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILATLIS 485

Query: 301 KFRFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS 334
           KF FTIS  YRHAP+VVLTI+PKYGVQV L P  S
Sbjct: 486 KFNFTISKNYRHAPIVVLTIKPKYGVQVILKPLVS 518

BLAST of Carg22272 vs. TAIR10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein)

HSP 1 Score: 249.6 bits (636), Expect = 2.7e-66
Identity = 132/337 (39.17%), Postives = 202/337 (59.94%), Query Frame = 0

Query: 6   ESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLCLPGSR 65
           E+G  E ++ ++ + LT D+ISRT F +  + G  +F L      LCA+A + + +PG R
Sbjct: 192 ENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIPGYR 251

Query: 66  FVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGR-SSC---YGSDLLGMLLNEMQQRK 125
           F P++ N +   L KE+ + L+++IE R+     G    C      DLLG+++    Q K
Sbjct: 252 FFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMI----QAK 311

Query: 126 NISTTTTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCNA 185
           N++       +Q I++ECK+FFFAG +TT+ LLTWT +LL+ +P WQ   R +V +VC +
Sbjct: 312 NVT-------VQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGS 371

Query: 186 R-TPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAI 245
           R  P+  H+ KL  L+M++NESLRLYPP     R A  D+KLG   IP G  + IP++A+
Sbjct: 372 RDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAV 431

Query: 246 HHSEELWGKDANQFNPNRFGAKAFGGGR----FIPFAGGPRNCIGQGFAIMEAKIILAMF 305
           HH + +WG D N+FNP RF        +    FIPF  G R CIGQ  AI++AK+ LA+ 
Sbjct: 432 HHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVM 491

Query: 306 LSKFRFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS 334
           + +F F ++ TY+HAP V++ + P++G  +     T+
Sbjct: 492 IQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRLTN 517

BLAST of Carg22272 vs. TAIR10
Match: AT3G14690.1 (cytochrome P450, family 72, subfamily A, polypeptide 15)

HSP 1 Score: 244.2 bits (622), Expect = 1.1e-64
Identity = 130/322 (40.37%), Postives = 200/322 (62.11%), Query Frame = 0

Query: 11  EFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLCLPGSRFVPSK 70
           E ++   +  +TAD+ISRT F +   +G +IF L   L +L  QA R   +PG  ++P+K
Sbjct: 194 EVDVWPGLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAFIPGYSYLPTK 253

Query: 71  YNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQRKNISTTTTTL 130
            NR +K+  +E++ +L  I+  R    E G +     DLLG+LL    ++    T    +
Sbjct: 254 SNRRMKAAAREIQVILRGIVNKRLRAREAGEAP--SDDLLGILLESNLRQ----TEGNGM 313

Query: 131 NLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCNARTPSIQHLS 190
           + + +M+ECK F+FAG ETT++LL WT++LL+ + +WQ   R +V QV   + P  + L+
Sbjct: 314 STEDLMEECKLFYFAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDAEGLN 373

Query: 191 KLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAIHHSEELWGKD 250
           +L ++ M++ E LRLYPP T L R   +++KLGD+ +P G+ I +P+L + H  ELWG D
Sbjct: 374 QLKVMTMILYEVLRLYPPVTQLTRAIHKELKLGDLTLPGGVQISLPILLVQHDIELWGND 433

Query: 251 ANQFNPNRFG---AKAFGGG-RFIPFAGGPRNCIGQGFAIMEAKIILAMFLSKFRFTISD 310
           A +FNP+RF    +KA      F PFA GPR CIGQ FA++EAK+ +A+ L +F F IS 
Sbjct: 434 AAEFNPDRFKDGLSKATKSQVSFFPFAWGPRICIGQNFALLEAKMAMALILRRFSFEISP 493

Query: 311 TYRHAPVVVLTIQPKYGVQVCL 329
           +Y HAP  V+TI P++G Q+ +
Sbjct: 494 SYVHAPYTVITIHPQFGAQLIM 509

BLAST of Carg22272 vs. TAIR10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2)

HSP 1 Score: 241.9 bits (616), Expect = 5.6e-64
Identity = 136/322 (42.24%), Postives = 193/322 (59.94%), Query Frame = 0

Query: 14  IADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLCLPGSRFVPSKYNR 73
           I+   K+LTADII+   F +   +G ++F     LQ+ CA A   L  PG +++P+  N 
Sbjct: 256 ISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCCAAALTDLYFPGIQYLPTPSNL 315

Query: 74  EIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQRKNISTTTTTLNLQ 133
           +I  L  +V   +  II++R        S  YG+DLLG++L      +    +   +++ 
Sbjct: 316 QIWKLDMKVNSSIKRIIDARL----TSESKDYGNDLLGIMLTAASSNE----SEKKMSID 375

Query: 134 LIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCNA-RTPSIQHLSKL 193
            I++ECKTFFFAGHETTA LLTW+ MLL+ + +WQ  +R +V   C   + P  +  SKL
Sbjct: 376 EIIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLREEVFNECGKDKIPDAETCSKL 435

Query: 194 TLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAIHHSEELWGKDAN 253
            L+N V  ESLRLY P   L R+A EDMKLG+++IPKG +I +P+  +H  + +WG DA+
Sbjct: 436 KLMNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKGTTIILPIAKMHRDKAVWGSDAD 495

Query: 254 QFNPNRF----GAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSKFRFTISDTY 313
           +FNP RF       A      + F+ GPR CIGQ FAIMEAK +LAM L +FR  +S  Y
Sbjct: 496 KFNPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAIMEAKTVLAMILQRFRLNLSADY 555

Query: 314 RHAPVVVLTIQPKYGVQVCLTP 331
           +HAP   LT+QP+Y + V L P
Sbjct: 556 KHAPADHLTLQPQYDLPVILEP 569

BLAST of Carg22272 vs. Swiss-Prot
Match: sp|Q9ZW95|C7352_ARATH (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 4.8e-129
Identity = 232/322 (72.05%), Postives = 265/322 (82.30%), Query Frame = 0

Query: 11  EFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLCLPGSRFVPSK 70
           E EI + M++LTADIISRTEF +  DKG ++F LLT+LQRLCAQA+RHLC PGSRF+PSK
Sbjct: 194 EVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQATRHLCFPGSRFLPSK 253

Query: 71  YNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQRKNISTTTTTL 130
           YNREIKSLK EVERLLMEII+SRKD VEIGRSS YG DLLG+LLN+M   KN       L
Sbjct: 254 YNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMDSNKN------NL 313

Query: 131 NLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCNA-RTPSIQHL 190
           N+Q+IMDECKTFFF GHETT+LLLTWT+MLLA NP WQ  VR +V QVC     PS++ L
Sbjct: 314 NVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGVPSVEQL 373

Query: 191 SKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAIHHSEELWGK 250
           S LT LN VINESLRLYPPAT+LPRMA ED+KLGD+ IPKGLSIWIPVLAIHHS ELWG+
Sbjct: 374 SSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHHSNELWGE 433

Query: 251 DANQFNPNRFGAKAFGGGR-FIPFAGGPRNCIGQGFAIMEAKIILAMFLSKFRFTISDTY 310
           DAN+FNP RF  ++F   R F+PFA GPRNCIGQ FA+MEAKIILAM +SKF F IS+ Y
Sbjct: 434 DANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKIILAMLVSKFSFAISENY 493

Query: 311 RHAPVVVLTIQPKYGVQVCLTP 331
           RHAP+VVLTI+PKYGVQ+ L P
Sbjct: 494 RHAPIVVLTIKPKYGVQLVLKP 509

BLAST of Carg22272 vs. Swiss-Prot
Match: sp|Q9FF18|C7351_ARATH (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 3.0e-107
Identity = 207/335 (61.79%), Postives = 239/335 (71.34%), Query Frame = 0

Query: 1   METELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLC 60
           +  E+  G  E EI + M +LTADIISRT+F +  +KG ++F+ LT+LQR CAQA+RHLC
Sbjct: 186 LRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQATRHLC 245

Query: 61  LPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQR 120
            PGSRF+PSKYNREIKSLKKEVERLL+EII+SR+D  E+GRSS +G DLLG+LLNEM   
Sbjct: 246 FPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMD-- 305

Query: 121 KNISTTTTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCN 180
            +                                    MLLA NP WQ  VR +V +V  
Sbjct: 306 IDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXMLLADNPTWQEKVREEVREVFG 365

Query: 181 AR-TPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLA 240
               PS+  LSKLT L+ VINESLRLYPPAT+LPRMA ED+KLGD+ IPKGLSIWIPVLA
Sbjct: 366 RNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLA 425

Query: 241 IHHSEELWGKDANQFNPNRFGAKAFGGGR-FIPFAGGPRNCIGQGFAIMEAKIILAMFLS 300
           IHHSEELWGKDANQFNP RFG + F  GR FIPFA GPRNCIGQ FA+MEAKIILA  +S
Sbjct: 426 IHHSEELWGKDANQFNPERFGGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILATLIS 485

Query: 301 KFRFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS 334
           KF FTIS  YRHAP+VVLTI+PKYGVQV L P  S
Sbjct: 486 KFNFTISKNYRHAPIVVLTIKPKYGVQVILKPLVS 518

BLAST of Carg22272 vs. Swiss-Prot
Match: sp|H2DH21|C7A29_PANGI (Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.8e-67
Identity = 130/326 (39.88%), Postives = 202/326 (61.96%), Query Frame = 0

Query: 8   GRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLCLPGSRFV 67
           G+ E ++  Y++ LT+D+ISRT F +  ++G +IF L      L  QAS+ + LPG RF+
Sbjct: 190 GQFELDVLPYLETLTSDVISRTAFGSSYEEGRKIFQLQREQAELIIQASQTIYLPGMRFL 249

Query: 68  PSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLL-NEMQQRKNISTT 127
           P+K N+ +K + KEV+  L  II  R   +E G  S +  DLLG+LL +  ++ K    T
Sbjct: 250 PTKRNKRMKEIAKEVKIALKSIINKRLKAMEAGERSSH-DDLLGILLESNSKEIKQHGNT 309

Query: 128 TTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCNARTPSI 187
              L +  +++ECK FFFAG ETT+ LL WT++LL+ + +WQ   + +V +      P  
Sbjct: 310 NFGLTVDEVIEECKLFFFAGQETTSNLLVWTMILLSQHQDWQKRAKEEVLRTFGNNKPDF 369

Query: 188 QHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAIHHSEEL 247
             L+ L ++NM++ E LRLYPP   L R   E++KLG++ +P G+ + +P++ +H+ +E+
Sbjct: 370 DGLNHLKVVNMILLEVLRLYPPILSLDRTIYEEIKLGEISLPAGVILLLPIILLHYDQEI 429

Query: 248 WGKDANQFNPNRFGAKAF----GGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSKFRF 307
           WG DA +FNP RF         G   + PF+ GPR CIGQ FA++EAK+ +AM L +F F
Sbjct: 430 WGDDAKEFNPERFSEGVLKATKGRVTYFPFSWGPRICIGQNFAMLEAKMAMAMILQRFSF 489

Query: 308 TISDTYRHAPVVVLTIQPKYGVQVCL 329
            +S +Y HAP  ++T+QP+YG  + L
Sbjct: 490 VLSPSYAHAPHAIITLQPQYGAHLIL 514

BLAST of Carg22272 vs. Swiss-Prot
Match: sp|O48786|C734A_ARATH (Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 4.9e-65
Identity = 132/337 (39.17%), Postives = 202/337 (59.94%), Query Frame = 0

Query: 6   ESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLCLPGSR 65
           E+G  E ++ ++ + LT D+ISRT F +  + G  +F L      LCA+A + + +PG R
Sbjct: 192 ENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIPGYR 251

Query: 66  FVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGR-SSC---YGSDLLGMLLNEMQQRK 125
           F P++ N +   L KE+ + L+++IE R+     G    C      DLLG+++    Q K
Sbjct: 252 FFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMI----QAK 311

Query: 126 NISTTTTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCNA 185
           N++       +Q I++ECK+FFFAG +TT+ LLTWT +LL+ +P WQ   R +V +VC +
Sbjct: 312 NVT-------VQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGS 371

Query: 186 R-TPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAI 245
           R  P+  H+ KL  L+M++NESLRLYPP     R A  D+KLG   IP G  + IP++A+
Sbjct: 372 RDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAV 431

Query: 246 HHSEELWGKDANQFNPNRFGAKAFGGGR----FIPFAGGPRNCIGQGFAIMEAKIILAMF 305
           HH + +WG D N+FNP RF        +    FIPF  G R CIGQ  AI++AK+ LA+ 
Sbjct: 432 HHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVM 491

Query: 306 LSKFRFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS 334
           + +F F ++ TY+HAP V++ + P++G  +     T+
Sbjct: 492 IQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRLTN 517

BLAST of Carg22272 vs. Swiss-Prot
Match: sp|B9X287|C7346_ORYSJ (Cytochrome P450 734A6 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A6 PE=2 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 4.1e-64
Identity = 132/337 (39.17%), Postives = 196/337 (58.16%), Query Frame = 0

Query: 6   ESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLCLPGSR 65
           +SG  E +++D+ + +T D I+RT F    + G  +F L   L    ++A R + +PG R
Sbjct: 192 KSGEVEIDVSDWFQVVTEDAITRTAFGRSYEDGKVVFKLQAQLMAFASEAFRKVFIPGYR 251

Query: 66  FVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQR---KN 125
           F+P+K N     L KE+ + L+ +I  R++  +  +      DLLG+++N        K 
Sbjct: 252 FLPTKKNTSSWKLDKEIRKNLVTLIGRRQEAGDDEKLDGCAKDLLGLMINAAASSNGGKR 311

Query: 126 ISTTTTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCNA- 185
            +   + + +  I++ECKTFFFAG +TT+ LLTW I++LA +P WQ   R +V  VC A 
Sbjct: 312 SALPVSPITVNDIVEECKTFFFAGKQTTSNLLTWAIVVLAMHPEWQERARQEVLDVCGAD 371

Query: 186 RTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGD-MDIPKGLSIWIPVLAI 245
             PS + L+KL  L M++NE+LRLYPPA    R A  D++LG  + IP+   + IP++A+
Sbjct: 372 GVPSREQLAKLKTLGMILNETLRLYPPAVATVRRAKADVELGGYLRIPRDTELLIPIMAV 431

Query: 246 HHSEELWGKDANQFNPNRFGAKAFGGGR----FIPFAGGPRNCIGQGFAIMEAKIILAMF 305
           HH   LWG DA QFNP RF        R    FIPF  G R CIGQ  AI+EAK+ +A+ 
Sbjct: 432 HHDARLWGPDAAQFNPARFAGGVARAARHPAAFIPFGLGARMCIGQNLAILEAKLTVAVI 491

Query: 306 LSKFRFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS 334
           L +F F +S  Y HAP V++ + P+YG  +   P +S
Sbjct: 492 LHRFEFRLSARYVHAPTVLMLLHPQYGAPIVFRPRSS 528

BLAST of Carg22272 vs. TrEMBL
Match: tr|A0A1S3CGC0|A0A1S3CGC0_CUCME (cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103500156 PE=4 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 4.3e-159
Identity = 282/334 (84.43%), Postives = 303/334 (90.72%), Query Frame = 0

Query: 1   METELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLC 60
           +ETEL+SGRTEFEIADYMKQLTADIISRTEFD +SDKGNQIFHLLTLLQ LCAQASRHLC
Sbjct: 187 LETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQHLCAQASRHLC 246

Query: 61  LPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQR 120
           LPGSRF PSKYNREIKSLK EVERLLMEII+SRKDGVEIGRS+CYGSDLLGMLLNEMQ++
Sbjct: 247 LPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRK 306

Query: 121 KNISTTTTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVC- 180
           K+      +LNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQ  VR +VNQVC 
Sbjct: 307 KDDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHEVNQVCH 366

Query: 181 NARTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLA 240
           N  TPS++HLSK TLLNMVINESLRLYPPATVLPRMA ED+K+GD++IPKGLSIWIPVLA
Sbjct: 367 NGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLA 426

Query: 241 IHHSEELWGKDANQFNPNRFGAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSK 300
           IHHSEELWGKD N+F P RF +K F GGRFIPFA GPRNC+GQGFA+MEAKIILAM LSK
Sbjct: 427 IHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCVGQGFALMEAKIILAMLLSK 486

Query: 301 FRFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS 334
           F FTISD+YRHAPV VLTIQPKYGVQV LTP  S
Sbjct: 487 FSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 520

BLAST of Carg22272 vs. TrEMBL
Match: tr|A0A0A0L6Y6|A0A0A0L6Y6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G271920 PE=4 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 4.8e-158
Identity = 281/334 (84.13%), Postives = 301/334 (90.12%), Query Frame = 0

Query: 1   METELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLC 60
           +ETE +SGRTEFEIADYMKQLTADIISRTEFD +SDKGNQIFHLLTLLQ LCAQASRHLC
Sbjct: 187 LETEFDSGRTEFEIADYMKQLTADIISRTEFDCNSDKGNQIFHLLTLLQHLCAQASRHLC 246

Query: 61  LPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQR 120
           LPGSRF PSKYNREIKSLK EVERLLMEII+SRKDGVEIGRS+CYGSDLLGMLLNEMQ+R
Sbjct: 247 LPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQKR 306

Query: 121 KNISTTTTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVC- 180
           +       +LNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQ  VR +VNQVC 
Sbjct: 307 REDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHEVNQVCH 366

Query: 181 NARTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLA 240
           N  TPS++HLSK TLLNMVINESLRLYPPATVLPRMA ED+K+GD++IPKGLSIWIPVLA
Sbjct: 367 NGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLA 426

Query: 241 IHHSEELWGKDANQFNPNRFGAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSK 300
           IHHSEELWGKD N+F P RF +K F GGRFIPFA GPRNCIGQGFA+MEAKIILAM LSK
Sbjct: 427 IHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKIILAMLLSK 486

Query: 301 FRFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS 334
           F F+ISD+YRHAPV VLTIQPKYGVQV LTP  S
Sbjct: 487 FSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINS 520

BLAST of Carg22272 vs. TrEMBL
Match: tr|A0A2C9WD27|A0A2C9WD27_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_02G068100 PE=4 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 1.1e-146
Identity = 261/331 (78.85%), Postives = 292/331 (88.22%), Query Frame = 0

Query: 1   METELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLC 60
           +E  +ESG TEFEI +YM +LTADIISRTEFD+  +KG QIFHLLT+LQ LCAQASRHLC
Sbjct: 186 LENAVESGETEFEIGEYMTRLTADIISRTEFDSSYEKGKQIFHLLTVLQSLCAQASRHLC 245

Query: 61  LPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQR 120
            PGSRF PSKYNREIKSLK EVERLLMEII+SRKD VEIGRSS YG+DLLGMLLNEMQ++
Sbjct: 246 FPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDCVEIGRSSSYGNDLLGMLLNEMQKK 305

Query: 121 KNISTTTTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCN 180
           +     + +LNLQLIMDECKTFFFAGHETTALLLTWT+MLLA+NP+WQ  VRA+V QVCN
Sbjct: 306 R--GNGSFSLNLQLIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQEKVRAEVKQVCN 365

Query: 181 ARTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAI 240
             TPS+ HL KLTLLNMVINESLRLYPPATVLPRMA ED KLGD+ IPKGLSIWIPVLAI
Sbjct: 366 GETPSVDHLPKLTLLNMVINESLRLYPPATVLPRMAFEDFKLGDLHIPKGLSIWIPVLAI 425

Query: 241 HHSEELWGKDANQFNPNRFGAKAFGGGR-FIPFAGGPRNCIGQGFAIMEAKIILAMFLSK 300
           HHSEELWGKDAN+FNP+RF +K+F  GR FIPFA GPRNCIGQ FA+MEAKIILAM +S+
Sbjct: 426 HHSEELWGKDANEFNPDRFASKSFAPGRHFIPFAAGPRNCIGQTFAMMEAKIILAMLISR 485

Query: 301 FRFTISDTYRHAPVVVLTIQPKYGVQVCLTP 331
           F FTISDTYRHAPV+VLTI+PKYGVQ+C+ P
Sbjct: 486 FSFTISDTYRHAPVIVLTIKPKYGVQICMKP 514

BLAST of Carg22272 vs. TrEMBL
Match: tr|B9RSK4|B9RSK4_RICCO (Cytochrome P450, putative OS=Ricinus communis OX=3988 GN=RCOM_1725340 PE=4 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.9e-146
Identity = 262/334 (78.44%), Postives = 291/334 (87.13%), Query Frame = 0

Query: 1   METELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLC 60
           ++  +ESG+TEFEI +YM +LTADIISRTEFD   +KG QIFHLL +LQRLCAQASRHLC
Sbjct: 184 LQKAVESGQTEFEIGEYMTRLTADIISRTEFDCSYEKGKQIFHLLNVLQRLCAQASRHLC 243

Query: 61  LPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQR 120
            PGSRF PSKYNREIKSLK EVERLLMEII+SRKD VEIGRSS YG+DLLGMLLNEMQ +
Sbjct: 244 FPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDCVEIGRSSTYGNDLLGMLLNEMQSK 303

Query: 121 KNISTTTTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCN 180
           +       +LNLQLIMDECKTFFFAGHETTALLLTWT+MLLA+NP+WQ  VRA+VNQVCN
Sbjct: 304 RE---NGFSLNLQLIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQEKVRAEVNQVCN 363

Query: 181 ARTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAI 240
             TPS+ HL KLTLLNMVINESLRLYPPATVLPRMA ED+KLGD+ IPKGLSIWIPVLAI
Sbjct: 364 GETPSVDHLPKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLHIPKGLSIWIPVLAI 423

Query: 241 HHSEELWGKDANQFNPNRFGAKAFGGGR-FIPFAGGPRNCIGQGFAIMEAKIILAMFLSK 300
           HHSEELWGKDAN+FNP+RF +K F  GR FIPFA GPRNCIGQ FA+MEAKIILAM +SK
Sbjct: 424 HHSEELWGKDANEFNPDRFASKTFAPGRHFIPFAAGPRNCIGQTFAMMEAKIILAMLISK 483

Query: 301 FRFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS 334
           F F ISD+YRHAPV+VLTI+PKYGVQVCL P  +
Sbjct: 484 FNFNISDSYRHAPVIVLTIKPKYGVQVCLRPLNN 514

BLAST of Carg22272 vs. TrEMBL
Match: tr|A0A059BSJ1|A0A059BSJ1_EUCGR (Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_F02489 PE=4 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 1.2e-145
Identity = 256/330 (77.58%), Postives = 295/330 (89.39%), Query Frame = 0

Query: 1   METELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLC 60
           ++  ++SG+TEFEI++YM +LTADIISRTEFD+  +KG QIFHLLTLLQ LCAQASRHLC
Sbjct: 155 LKKAVDSGQTEFEISEYMARLTADIISRTEFDSSYEKGKQIFHLLTLLQNLCAQASRHLC 214

Query: 61  LPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQR 120
           LPGSRF PSKYNREIKSLK EVERLLMEII+SR+D VE+GRSS YG+DLLGMLLNEMQ++
Sbjct: 215 LPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRRDCVEVGRSSSYGNDLLGMLLNEMQKK 274

Query: 121 KNISTTTTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCN 180
           +  S    +LNLQLIMDECKTFFFAGH+TTALLLTWT+MLLA+NP+WQ  VR +V QVCN
Sbjct: 275 R--SGNGFSLNLQLIMDECKTFFFAGHDTTALLLTWTVMLLASNPSWQERVREEVAQVCN 334

Query: 181 ARTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAI 240
              PS++HLSKLTLLNMVINESLRLYPPA+VLPRMA ED+KLGD+ IPKGLSIWIPVLAI
Sbjct: 335 GADPSVEHLSKLTLLNMVINESLRLYPPASVLPRMAFEDIKLGDLHIPKGLSIWIPVLAI 394

Query: 241 HHSEELWGKDANQFNPNRFGAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSKF 300
           HHSEELWGKDAN+FNP RF +KAF  GRF+PFA GPRNCIGQ FA+MEAKIILAM +S+F
Sbjct: 395 HHSEELWGKDANEFNPERFASKAFTPGRFVPFAMGPRNCIGQSFALMEAKIILAMLISRF 454

Query: 301 RFTISDTYRHAPVVVLTIQPKYGVQVCLTP 331
            FTIS++YRHAPV+VLTI+PKYGVQ+CL P
Sbjct: 455 SFTISESYRHAPVIVLTIKPKYGVQICLKP 482

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022934803.12.0e-18498.80cytokinin hydroxylase [Cucurbita moschata][more]
XP_022983651.19.3e-18297.00cytokinin hydroxylase-like [Cucurbita maxima][more]
XP_023528555.15.7e-17996.11cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo][more]
XP_008461587.16.5e-15984.43PREDICTED: cytokinin hydroxylase-like [Cucumis melo][more]
XP_022148131.11.1e-15884.68cytokinin hydroxylase-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G67110.12.7e-13072.05cytochrome P450, family 735, subfamily A, polypeptide 2[more]
AT5G38450.11.7e-10861.79cytochrome P450, family 735, subfamily A, polypeptide 1[more]
AT2G26710.12.7e-6639.17Cytochrome P450 superfamily protein[more]
AT3G14690.11.1e-6440.37cytochrome P450, family 72, subfamily A, polypeptide 15[more]
AT2G46950.15.6e-6442.24cytochrome P450, family 709, subfamily B, polypeptide 2[more]
Match NameE-valueIdentityDescription
sp|Q9ZW95|C7352_ARATH4.8e-12972.05Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1[more]
sp|Q9FF18|C7351_ARATH3.0e-10761.79Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1[more]
sp|H2DH21|C7A29_PANGI1.8e-6739.88Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1[more]
sp|O48786|C734A_ARATH4.9e-6539.17Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1[more]
sp|B9X287|C7346_ORYSJ4.1e-6439.17Cytochrome P450 734A6 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A6 PE=2 ... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3CGC0|A0A1S3CGC0_CUCME4.3e-15984.43cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103500156 PE=4 SV=1[more]
tr|A0A0A0L6Y6|A0A0A0L6Y6_CUCSA4.8e-15884.13Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G271920 PE=4 SV=1[more]
tr|A0A2C9WD27|A0A2C9WD27_MANES1.1e-14678.85Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_02G068100 PE=4 SV=... [more]
tr|B9RSK4|B9RSK4_RICCO1.9e-14678.44Cytochrome P450, putative OS=Ricinus communis OX=3988 GN=RCOM_1725340 PE=4 SV=1[more]
tr|A0A059BSJ1|A0A059BSJ1_EUCGR1.2e-14577.58Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_F02489 PE=4 SV... [more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0005506iron ion binding
GO:0020037heme binding
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Vocabulary: INTERPRO
TermDefinition
IPR036396Cyt_P450_sf
IPR002401Cyt_P450_E_grp-I
IPR001128Cyt_P450
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg22272-RACarg22272-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 75..95
NoneNo IPR availablePANTHERPTHR24282:SF56CYTOKININ HYDROXYLASEcoord: 2..332
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 2..332
IPR001128Cytochrome P450PRINTSPR00385P450coord: 197..208
score: 46.75
coord: 279..290
score: 43.89
coord: 270..279
score: 55.42
coord: 145..162
score: 40.88
IPR001128Cytochrome P450PFAMPF00067p450coord: 9..306
e-value: 1.1E-66
score: 225.5
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 134..151
score: 29.73
coord: 279..302
score: 31.75
coord: 154..180
score: 22.5
coord: 269..279
score: 48.91
IPR036396Cytochrome P450 superfamilyGENE3DG3DSA:1.10.630.10coord: 1..330
e-value: 1.6E-86
score: 292.7
IPR036396Cytochrome P450 superfamilySUPERFAMILYSSF48264Cytochrome P450coord: 8..330