Cucsa.017520 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.017520
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Unplaced genomic scaffold scaffold_6839, whole genome shotgun sequence
Location	scaffold00245 : 293670 .. 293883 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCTCGACGTAAAGAAGAAGTGTTTAACTATCGACATTCTTCACTGCAAAATGTCATTGAACATTGTTTTATTGTGCTGAAGGTTCAATTTCTTATTATTAAGCAAATGCCTCCATACCCGATCGAGACACAAAAATATATACCGATAGCATGTTACACAATTCACAACTATATTAGATTGAATGATTGTCAAGATGATCTATTCAATGACTAA

mRNA sequence

tcctcgacgtaaagaagaagtgtttaactatcgacattcttcactgcaaaatgtcattgaacattgttttattgtgctgaaggttcaatttcttattattaagcaaatgcctccatacccgatcgagacacaaaaatatataccgatagcatgttacacaattcacaactatattagattgaatgattgtcaagatgatctattcaatgactaa

Coding sequence (CDS)

TCCTCGACGTAAAGAAGAAGTGTTTAACTATCGACATTCTTCACTGCAAAATGTCATTGAACATTGTTTTATTGTGCTGAAGGTTCAATTTCTTATTATTAAGCAAATGCCTCCATACCCGATCGAGACACAAAAATATATACCGATAGCATGTTACACAATTCACAACTATATTAGATTGAATGATTGTCAAGATGATCTATTCAATGACTAA

Protein sequence

PRRKEEVFNYRHSSLQNVIEHCFIVLKVQFLIIKQMPPYPIETQKYIPIACYTIHNYIRLNDCQDDLFND*

Homology

[Close]

BLAST of Cucsa.017520 vs. TrEMBL
Match: A0A0B2SJL0_GLYSO (Putative nuclease HARBI1 (Fragment) OS=Glycine soja GN=glysoja_043532 PE=4 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 1.5e-15
Identity = 43/68 (63.24%), Postives = 52/68 (76.47%), Query Frame = 1


Query: 1   PRRKEEVFNYRHSSLQNVIEHCFIVLKVQFLIIKQMPPYPIETQKYIPIACYTIHNYIRL 60
           PR KEE+FNYRHSSL+NVIE CF VLK +F I+K MP YPI  Q+ I IAC TIHN+IR+
Sbjct: 246 PRGKEELFNYRHSSLRNVIERCFGVLKARFPILKLMPSYPIRRQRLILIACCTIHNFIRM 305

Query: 61  NDCQDDLF 69
            +  + LF
Sbjct: 306 QNGANTLF 313

[Close]

BLAST of Cucsa.017520 vs. TrEMBL
Match: A0A0R0GE91_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_14G172000 PE=4 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 8.3e-14
Identity = 41/69 (59.42%), Postives = 50/69 (72.46%), Query Frame = 1


Query: 1   PRRKEEVFNYRHSSLQNVIEHCFIVLKVQFLIIKQMPPYPIETQKYIPIACYTIHNYIRL 60
           P  KEE+FNYR SSL+NVIE C  VLK +F I+K MP YPI  Q+ I IAC TIHN+IR+
Sbjct: 203 PHGKEELFNYRQSSLRNVIERCVGVLKARFPILKLMPSYPIRRQRLILIACCTIHNFIRM 262

Query: 61  NDCQDDLFN 70
            +  D LF+
Sbjct: 263 QNGVDTLFD 271

[Close]

BLAST of Cucsa.017520 vs. TrEMBL
Match: K7M7K9_SOYBN (Uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 8.3e-14
Identity = 41/69 (59.42%), Postives = 50/69 (72.46%), Query Frame = 1


Query: 1   PRRKEEVFNYRHSSLQNVIEHCFIVLKVQFLIIKQMPPYPIETQKYIPIACYTIHNYIRL 60
           P  KEE+FNYR SSL+NVIE C  VLK +F I+K MP YPI  Q+ I IAC TIHN+IR+
Sbjct: 205 PHGKEELFNYRQSSLRNVIERCVGVLKARFPILKLMPSYPIRRQRLILIACCTIHNFIRM 264

Query: 61  NDCQDDLFN 70
            +  D LF+
Sbjct: 265 QNGVDTLFD 273

[Close]

BLAST of Cucsa.017520 vs. TrEMBL
Match: B9FTH0_ORYSJ (Reticulon-like protein OS=Oryza sativa subsp. japonica GN=OsJ_21474 PE=4 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 3.5e-12
Identity = 38/68 (55.88%), Postives = 48/68 (70.59%), Query Frame = 1


Query: 1   PRRKEEVFNYRHSSLQNVIEHCFIVLKVQFLIIKQMPPYPIETQKYIPIACYTIHNYIRL 60
           P  KEEVFNY HSSL+NV+E  F VLK ++ I+K +P YP ETQ +I +AC  +HNYIRL
Sbjct: 727 PEGKEEVFNYAHSSLRNVMERSFGVLKQKWRILKYVPSYPPETQTHIIVACIALHNYIRL 786

Query: 61  NDCQDDLF 69
           +   D  F
Sbjct: 787 SGLTDRHF 794

[Close]

BLAST of Cucsa.017520 vs. TrEMBL
Match: B9FWG5_ORYSJ (Uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_23740 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.3e-11
Identity = 38/68 (55.88%), Postives = 47/68 (69.12%), Query Frame = 1


Query: 1   PRRKEEVFNYRHSSLQNVIEHCFIVLKVQFLIIKQMPPYPIETQKYIPIACYTIHNYIRL 60
           P  KEEVFNY HSSL+NVIE  F VLK ++ I+K +P YP E Q +I +AC  +HNYIRL
Sbjct: 524 PEGKEEVFNYAHSSLRNVIERSFGVLKQKWRILKYVPCYPREIQTHIIVACMALHNYIRL 583

Query: 61  NDCQDDLF 69
              +D  F
Sbjct: 584 AGLRDKHF 591

[Close]

BLAST of Cucsa.017520 vs. TAIR10
Match: AT5G35695.1 (AT5G35695.1 Putative harbinger transposase-derived nuclease (InterPro:IPR006912))

HSP 1 Score: 58.2 bits (139), Expect = 2.5e-09
Identity = 26/66 (39.39%), Postives = 39/66 (59.09%), Query Frame = 1


Query: 1   PRRKEEVFNYRHSSLQNVIEHCFIVLKVQFLIIKQMPPYPIETQKYIPIACYTIHNYIRL 60
           P    E+FN RH SL+NVIE  F + K +F I K  PP+  + Q  + + C  +HN++R 
Sbjct: 83  PETPHELFNLRHVSLRNVIERIFGIFKSRFAIFKSAPPFSYKKQAGLVLTCAALHNFLR- 142

Query: 61  NDCQDD 67
            +C+ D
Sbjct: 143 KECRSD 147

[Close]

BLAST of Cucsa.017520 vs. TAIR10
Match: AT5G41980.1 (AT5G41980.1 Putative harbinger transposase-derived nuclease (InterPro:IPR006912))

HSP 1 Score: 56.6 bits (135), Expect = 7.2e-09
Identity = 27/64 (42.19%), Postives = 37/64 (57.81%), Query Frame = 1


Query: 5   EEVFNYRHSSLQNVIEHCFIVLKVQFLIIKQMPPYPIETQKYIPIACYTIHNYIRLNDCQ 64
           +E+FN RH  L   I   F  LK +F I+   PPYP++TQ  + IA   +HNY+RL    
Sbjct: 248 KEMFNERHKLLHRAIHRTFGALKERFPILLSAPPYPLQTQVKLVIAACALHNYVRLEKPD 307

Query: 65  DDLF 69
           D +F
Sbjct: 308 DLVF 311

[Close]

BLAST of Cucsa.017520 vs. NCBI nr
Match: gi|659086645|ref|XP_008444045.1| (PREDICTED: uncharacterized protein LOC103487496 [Cucumis melo])

HSP 1 Score: 124.4 bits (311), Expect = 7.9e-26
Identity = 57/69 (82.61%), Postives = 62/69 (89.86%), Query Frame = 1


Query: 1   PRRKEEVFNYRHSSLQNVIEHCFIVLKVQFLIIKQMPPYPIETQKYIPIACYTIHNYIRL 60
           PR +EE FNYRHSSL+NVIE CF VLK QFLI+KQMPPYPI+TQKYIPIAC T+HNYIRL
Sbjct: 41  PRGREEGFNYRHSSLRNVIERCFCVLKAQFLILKQMPPYPIKTQKYIPIACCTVHNYIRL 100

Query: 61  NDCQDDLFN 70
           ND QDDLFN
Sbjct: 101 NDRQDDLFN 109

[Close]

BLAST of Cucsa.017520 vs. NCBI nr
Match: gi|659111926|ref|XP_008455977.1| (PREDICTED: uncharacterized protein LOC103496041 [Cucumis melo])

HSP 1 Score: 114.0 bits (284), Expect = 1.1e-22
Identity = 54/68 (79.41%), Postives = 58/68 (85.29%), Query Frame = 1


Query: 3   RKEEVFNYRHSSLQNVIEHCFIVLKVQFLIIKQMPPYPIETQKYIPIACYTIHNYIRLND 62
           R  EVFNYRHSSL+NVIE CF VLK +F I+KQMPPYPI+T KYI IAC TIHNYIRLND
Sbjct: 134 RGREVFNYRHSSLRNVIERCFGVLKARFPILKQMPPYPIKTHKYISIACCTIHNYIRLND 193

Query: 63  CQDDLFND 71
            QDDLFND
Sbjct: 194 RQDDLFND 201

[Close]

BLAST of Cucsa.017520 vs. NCBI nr
Match: gi|734429578|gb|KHN45215.1| (Putative nuclease HARBI1, partial [Glycine soja])

HSP 1 Score: 89.7 bits (221), Expect = 2.2e-15
Identity = 43/68 (63.24%), Postives = 52/68 (76.47%), Query Frame = 1


Query: 1   PRRKEEVFNYRHSSLQNVIEHCFIVLKVQFLIIKQMPPYPIETQKYIPIACYTIHNYIRL 60
           PR KEE+FNYRHSSL+NVIE CF VLK +F I+K MP YPI  Q+ I IAC TIHN+IR+
Sbjct: 246 PRGKEELFNYRHSSLRNVIERCFGVLKARFPILKLMPSYPIRRQRLILIACCTIHNFIRM 305

Query: 61  NDCQDDLF 69
            +  + LF
Sbjct: 306 QNGANTLF 313

[Close]

BLAST of Cucsa.017520 vs. NCBI nr
Match: gi|828289035|ref|XP_012570899.1| (PREDICTED: uncharacterized protein LOC105852030 [Cicer arietinum])

HSP 1 Score: 84.3 bits (207), Expect = 9.1e-14
Identity = 42/69 (60.87%), Postives = 49/69 (71.01%), Query Frame = 1


Query: 1   PRRKEEVFNYRHSSLQNVIEHCFIVLKVQFLIIKQMPPYPIETQKYIPIACYTIHNYIRL 60
           PR  EE+FNYRHSSL+  IE CF VLK +F I+K MPPY    Q+ I IAC TIHNYIR 
Sbjct: 288 PRSPEELFNYRHSSLRMTIERCFGVLKNRFPILKLMPPYKPSRQRLIVIACCTIHNYIRK 347

Query: 61  NDCQDDLFN 70
            +  D+LFN
Sbjct: 348 WNLPDELFN 356

[Close]

BLAST of Cucsa.017520 vs. NCBI nr
Match: gi|828290881|ref|XP_012573888.1| (PREDICTED: uncharacterized protein LOC105852522 [Cicer arietinum])

HSP 1 Score: 84.3 bits (207), Expect = 9.1e-14
Identity = 42/69 (60.87%), Postives = 49/69 (71.01%), Query Frame = 1


Query: 1   PRRKEEVFNYRHSSLQNVIEHCFIVLKVQFLIIKQMPPYPIETQKYIPIACYTIHNYIRL 60
           PR  EE+FNYRHSSL+  IE CF VLK +F I+K MPPY    Q+ I IAC TIHNYIR 
Sbjct: 298 PRSPEELFNYRHSSLRMTIERCFGVLKNRFPILKLMPPYKPSRQRLIVIACCTIHNYIRK 357

Query: 61  NDCQDDLFN 70
            +  D+LFN
Sbjct: 358 WNLPDELFN 366

The following BLAST results are available for this feature:

BLAST of Cucsa.017520 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0B2SJL0_GLYSO	1.5e-15	63.24	Putative nuclease HARBI1 (Fragment) OS=Glycine soja GN=glysoja_043532 PE=4 SV=1	[more]
A0A0R0GE91_SOYBN	8.3e-14	59.42	Uncharacterized protein OS=Glycine max GN=GLYMA_14G172000 PE=4 SV=1	[more]
K7M7K9_SOYBN	8.3e-14	59.42	Uncharacterized protein OS=Glycine max PE=4 SV=1	[more]
B9FTH0_ORYSJ	3.5e-12	55.88	Reticulon-like protein OS=Oryza sativa subsp. japonica GN=OsJ_21474 PE=4 SV=1	[more]
B9FWG5_ORYSJ	1.3e-11	55.88	Uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_23740 PE=4 SV=1	[more]

BLAST of Cucsa.017520 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G35695.1	2.5e-09	39.39	Putative harbinger transposase-derived nuclease (InterPro:IPR006912)	[more]
AT5G41980.1	7.2e-09	42.19	Putative harbinger transposase-derived nuclease (InterPro:IPR006912)	[more]

BLAST of Cucsa.017520 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659086645\|ref\|XP_008444045.1\|	7.9e-26	82.61	PREDICTED: uncharacterized protein LOC103487496 [Cucumis melo]	[more]
gi\|659111926\|ref\|XP_008455977.1\|	1.1e-22	79.41	PREDICTED: uncharacterized protein LOC103496041 [Cucumis melo]	[more]
gi\|734429578\|gb\|KHN45215.1\|	2.2e-15	63.24	Putative nuclease HARBI1, partial [Glycine soja]	[more]
gi\|828289035\|ref\|XP_012570899.1\|	9.1e-14	60.87	PREDICTED: uncharacterized protein LOC105852030 [Cicer arietinum]	[more]
gi\|828290881\|ref\|XP_012573888.1\|	9.1e-14	60.87	PREDICTED: uncharacterized protein LOC105852522 [Cicer arietinum]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR027806	HARBI1_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0016020	membrane
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.017520.1	Cucsa.017520.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027806	Harbinger transposase-derived nuclease domain	PFAM	PF13359	DDE_Tnp_4	coord: 4..56 score: 1.
None	No IPR available	PANTHER	PTHR22930	UNCHARACTERIZED	coord: 1..69 score: 3.8
None	No IPR available	PANTHER	PTHR22930:SF33	SUBFAMILY NOT NAMED	coord: 1..69 score: 3.8

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.017520	Melon (DHL92) v3.6.1	cgymedB435
Cucsa.017520	Silver-seed gourd	carcgyB1169
Cucsa.017520	Cucumber (Chinese Long) v3	cgycucB401
Cucsa.017520	Wax gourd	cgywgoB534
Cucsa.017520	Cucurbita maxima (Rimu)	cgycmaB0734
Cucsa.017520	Cucurbita maxima (Rimu)	cgycmaB0735
Cucsa.017520	Cucurbita moschata (Rifu)	cgycmoB0723
Cucsa.017520	Cucurbita moschata (Rifu)	cgycmoB0724
Cucsa.017520	Wild cucumber (PI 183967)	cgycpiB396
Cucsa.017520	Cucumber (Chinese Long) v2	cgycuB376
Cucsa.017520	Melon (DHL92) v3.5.1	cgymeB440
Cucsa.017520	Cucurbita pepo (Zucchini)	cgycpeB0689
Cucsa.017520	Cucurbita pepo (Zucchini)	cgycpeB0690
Cucsa.017520	Bottle gourd (USVL1VR-Ls)	cgylsiB424