Cucsa.012920 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.012920 vs. Swiss-Prot
Match: DHFS_ARATH (Dihydrofolate synthetase OS=Arabidopsis thaliana GN=DHFS PE=1 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 6.8e-156
Identity = 305/504 (60.52%), Postives = 378/504 (75.00%), Query Frame = 1

Query: 1   MTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKG 60
           +  F+ +L+SLKNYEK GVP+G+GTDSDDGFDLGRM+RLM RL NP  ++K +H+AGTKG
Sbjct: 45  LRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHVAGTKG 104

Query: 61  KGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFD 120
           KGST+AFLSNILR  GYSVGCY+SPHI +I+ERIS   +G+ VS   LN LF   K + +
Sbjct: 105 KGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSVKPILE 164

Query: 121 QSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITS 180
           QS++ ENG LSHFE+LT +AFSLF +E+VD+AVIEAGLGGARDATN+I SS LAA+VIT+
Sbjct: 165 QSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGARDATNVIESSNLAASVITT 224

Query: 181 IGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASD 240
           IGEEH+AALGGSLESIA AK+GIIK G P +LGGPFLP IE ILR KA S+SS VI AS+
Sbjct: 225 IGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIEGILRSKAASVSSSVILASN 284

Query: 241 PGNRSTIKGVNLLNG-GLSQCCDIVIQ---IDNEFIELLDVNLRMLGPHQLQNAATATCV 300
            G+ S+IKG+   NG GL Q CDIVIQ    D   +EL DVNLRMLG HQLQNA TATCV
Sbjct: 285 IGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDVNLRMLGHHQLQNAVTATCV 344

Query: 301 ILTLRNLG-WRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAK 360
            L LR+ G  R++D +IR GLE T L+GRS FL  +EAE L LPGAT+LLDGAHTK+SA+
Sbjct: 345 SLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAETLLLPGATVLLDGAHTKESAR 404

Query: 361 ALLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSA 420
           AL + I+  FPE +L  VVAMASDK+HV FA+E L G K E+V+LTEA IGGGK R+T +
Sbjct: 405 ALKEMIKKDFPEKRLVFVVAMASDKDHVSFAKELLSGLKPEAVILTEADIGGGKIRSTES 464

Query: 421 AFLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILK-QR 480
           + L++ WI+A++ELG   S+E  +                   ++L ++K+A +IL    
Sbjct: 465 SVLKESWIKAADELG-SRSMEASE-----------------NKTVLGSLKLAYKILSDDT 524

Query: 481 TKGRQGLVVVSGSLHAVSMVLASL 499
           T    G+V+V+GSLH VS VLASL
Sbjct: 525 TSSDSGMVIVTGSLHIVSSVLASL 528

BLAST of Cucsa.012920 vs. Swiss-Prot
Match: FOLC_BACSU (Folylpolyglutamate synthase OS=Bacillus subtilis (strain 168) GN=folC PE=3 SV=2)

HSP 1 Score: 178.7 bits (452), Expect = 1.6e-43
Identity = 122/351 (34.76%), Postives = 187/351 (53.28%), Query Frame = 1

Query: 33  LGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNILRVEGYSVGCYTSPHIETIRE 92
           LGRM++LM RLG+P+ + +A H+AGT GKGST AF+ ++L+  GY+VG +TSP+I T  E
Sbjct: 24  LGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNE 83

Query: 93  RISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSHFEVLTAMAFSLFAQ-EDVDV 152
           RIS+  +G  +S +   +L  + K   +   + E G  + FE++TA AF  FA+   VD 
Sbjct: 84  RISV--NGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIMTACAFLYFAEFHKVDF 143

Query: 153 AVIEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGSLESIATAKAGIIKRGCPTI 212
            + E GLGG  D+TN++   E    VITSIG +H+  LG ++E IA  KAGIIK G P I
Sbjct: 144 VIFETGLGGRFDSTNVV---EPLLTVITSIGHDHMNILGNTIEEIAGEKAGIIKEGIP-I 203

Query: 213 LGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNEF 272
           +     P    ++R +A   ++P  S  D      I     L  G         + + + 
Sbjct: 204 VTAVTQPEALQVIRHEAERHAAPFQSLHD---ACVIFNEEALPAGE----QFSFKTEEKC 263

Query: 273 IELLDVNLRMLGPHQLQNAATATCVI--LTLRNLGWRISDASIRSGLEQTFLIGRSHFLA 332
            E  D+   ++G HQ QNAA +      L   N+   ISD ++RSGL +    GR   + 
Sbjct: 264 YE--DIRTSLIGTHQRQNAALSILAAEWLNKENIA-HISDEALRSGLVKAAWPGRLELVQ 323

Query: 333 AREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLALVVAMASDK 381
                        + LDGAH ++  + L +T++  F  +++++V +   DK
Sbjct: 324 EH---------PPVYLDGAHNEEGVEKLAETMKQRFANSRISVVFSALKDK 349

BLAST of Cucsa.012920 vs. Swiss-Prot
Match: FOLCP_HALVD (Probable bifunctional folylpolyglutamate synthase/dihydropteroate synthase OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) GN=folCP PE=3 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.1e-31
Identity = 109/349 (31.23%), Postives = 170/349 (48.71%), Query Frame = 1

Query: 36  MRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNILRVEGYSVGCYTSPHIETIRERIS 95
           +RRL+  LG+P      + +AG+ GKGSTA  +  +LR  G  VG YTSPH + +RER+ 
Sbjct: 25  IRRLLSHLGDPHEGVSFVQVAGSNGKGSTARMVDAMLRESGAHVGLYTSPHFDDVRERVR 84

Query: 96  LGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIE 155
           +   G  +   AL++     K    +    +   L+ FE +TA+A   F +  VDVAV+E
Sbjct: 85  V--DGRKIPKSALSAFVAEAKPYLVERA-ADGEPLTFFETVTALALWYFDRAGVDVAVLE 144

Query: 156 AGLGGARDATNIICSSELAAAVITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILG-- 215
            G+GG  DAT+ +   +  A+ +T++  EH A LG ++  IA  KA +     P + G  
Sbjct: 145 VGMGGELDATSAV---DPVASAVTNVSLEHTAVLGDTVAEIAKTKAAVAPADAPLVTGTT 204

Query: 216 GPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIE 275
           G  L     ++R++A      V++  D  +  +   V +  GG     +  + ++ +  E
Sbjct: 205 GEALS----VIREEA----GDVLTVGD-ADADSDADVRVSYGGRVNHQEAAVTVETD-AE 264

Query: 276 LLDVNLRMLGPHQLQNAATATCVILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREA 335
            LDV + +LG +Q +NA  A  +   +R     I   +I  GL      GR         
Sbjct: 265 TLDVRIPLLGAYQARNAGIAVSLARQVRP---DIDAEAIHRGLRNAHWPGR--------F 324

Query: 336 EVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLALVVAMASDKNH 383
           EV+G    T++LDGAH  D A A + T+   F    L LV     DK+H
Sbjct: 325 EVMGTE-PTVVLDGAHNPD-ACAQVATVLDEFDYDDLHLVYGAMHDKDH 344

BLAST of Cucsa.012920 vs. Swiss-Prot
Match: FOLC_LACCA (Folylpolyglutamate synthase OS=Lactobacillus casei GN=fgs PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.6e-30
Identity = 100/356 (28.09%), Postives = 174/356 (48.88%), Query Frame = 1

Query: 32  DLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNILRVEGYSVGCYTSPHIETIR 91
           D  R+  L+  LGNPQ + + IH+ GT GKGS A  ++++L   G +VG YTSP I    
Sbjct: 21  DHRRILTLLHALGNPQQQGRYIHVTGTNGKGSAANAIAHVLEASGLTVGLYTSPFIMRFN 80

Query: 92  ERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSHFEVLTAMAFSLFAQEDVDV 151
           ERI +     +     +N++      +     +  + +++ FE +TA+ +  F Q  VDV
Sbjct: 81  ERIMIDHE-PIPDAALVNAVAFVRAALERLQQQQADFNVTEFEFITALGYWYFRQRQVDV 140

Query: 152 AVIEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGSLESIATAKAGIIKRGCPTI 211
           AVIE G+GG  D+TN+I       +V+T +  +H   LG ++ +IA  KAGIIKRG P +
Sbjct: 141 AVIEVGIGGDTDSTNVITP---VVSVLTEVALDHQKLLGHTITAIAKHKAGIIKRGIPVV 200

Query: 212 LGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNEF 271
            G   +P    ++  K  +  S  +      +    K    L+G   +        +++ 
Sbjct: 201 TGN-LVPDAAAVVAAKVATTGSQWLRFDRDFSVPKAK----LHGWGQR-----FTYEDQD 260

Query: 272 IELLDVNLRMLGPHQLQNAATA-TCVILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAA 331
             + D+ + ++G +Q +N A A     +  +   W ++  +IR GL        SH+  A
Sbjct: 261 GRISDLEVPLVGDYQQRNMAIAIQTAKVYAKQTEWPLTPQNIRQGL------AASHW-PA 320

Query: 332 REAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLALVVAMASDKNHVGFA 387
           R  ++   P   I++DGAH  D    L+  ++  F +  + ++  + +DK++   A
Sbjct: 321 RLEKISDTP--LIVIDGAHNPDGINGLITALKQLFSQ-PITVIAGILADKDYAAMA 352

BLAST of Cucsa.012920 vs. Swiss-Prot
Match: FOLD_YEAST (Dihydrofolate synthetase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=FOL3 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.7e-29
Identity = 107/374 (28.61%), Postives = 182/374 (48.66%), Query Frame = 1

Query: 33  LGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNILRVEGYSVGCYTSPHIETIRE 92
           L R+ +L+E LGNPQ+  + +HIAGT GKGS   +LS++L+ + Y +G +T+PH+  + +
Sbjct: 7   LSRITKLLEHLGNPQNSLRVLHIAGTNGKGSVCTYLSSVLQQKSYQIGKFTTPHLVHVTD 66

Query: 93  RISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGH---LSHFEVLTAMAFSLFAQEDV 152
            I+       ++ K +    +R + +  Q   L   H    + FE+LT  AF  F     
Sbjct: 67  SIT-------INNKPIP--LERYQNIRLQLEALNKSHSLKCTEFELLTCTAFKYFYDVQC 126

Query: 153 DVAVIEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGSLESIATAKAGIIKRGCP 212
              VIE GLGG  DATN+I  +  A   IT I  +H + LG +L  I+  KAGII  G P
Sbjct: 127 QWCVIEVGLGGRLDATNVIPGANKACCGITKISLDHESFLGNTLSEISKEKAGIITEGVP 186

Query: 213 -TILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNLLNGGLSQCCDIVIQID 272
            T++ G     +  +++++  ++ S  +S +D    S + G N+++     C D+     
Sbjct: 187 FTVIDGTNEASVINVVKERCKALGSE-LSVTD----SQLNG-NMIDTNSWGCFDL----- 246

Query: 273 NEFIELLDVNLRMLGPHQLQNAATATCVILTLR-NLGWRISDASIRSGLEQTFLIGRSHF 332
                     L + G +Q+ N   A  ++  L+ N    I+   + + L +    GR + 
Sbjct: 247 --------AKLPLNGEYQIFNLRVAMGMLDYLQMNELIDITKNEVSTRLAKVDWPGRLYR 306

Query: 333 LAAREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLALVVAMASDKNHVGFAR 392
           +  R  +V       IL+DGAH   +A  L+  ++  +    L  V+A+   KN     +
Sbjct: 307 MDYRFDKVSNRT-VPILMDGAHNGSAAVELVKYLRKEYGNQPLTFVMAVTHGKNLEPLLQ 349

Query: 393 EFLQGGKLESVVLT 402
             L+   ++ V+LT
Sbjct: 367 PLLR--PIDQVILT 349

BLAST of Cucsa.012920 vs. TrEMBL
Match: A0A0A0L7A4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G354540 PE=4 SV=1)

HSP 1 Score: 966.1 bits (2496), Expect = 1.7e-278
Identity = 500/500 (100.00%), Postives = 500/500 (100.00%), Query Frame = 1

Query: 1   MTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKG 60
           MTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKG
Sbjct: 49  MTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKG 108

Query: 61  KGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFD 120
           KGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFD
Sbjct: 109 KGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFD 168

Query: 121 QSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITS 180
           QSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITS
Sbjct: 169 QSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITS 228

Query: 181 IGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASD 240
           IGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASD
Sbjct: 229 IGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASD 288

Query: 241 PGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTL 300
           PGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTL
Sbjct: 289 PGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTL 348

Query: 301 RNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDT 360
           RNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDT
Sbjct: 349 RNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDT 408

Query: 361 IQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRD 420
           IQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRD
Sbjct: 409 IQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRD 468

Query: 421 CWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQG 480
           CWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQG
Sbjct: 469 CWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQG 528

Query: 481 LVVVSGSLHAVSMVLASLHS 501
           LVVVSGSLHAVSMVLASLHS
Sbjct: 529 LVVVSGSLHAVSMVLASLHS 548

BLAST of Cucsa.012920 vs. TrEMBL
Match: E0CUK0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0050g02490 PE=4 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 1.2e-186
Identity = 340/509 (66.80%), Postives = 409/509 (80.35%), Query Frame = 1

Query: 1   MTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKG 60
           +  F+ YLD+LKNYEK GVP+ +GTDS  GFDLGRM RLM+RLGNP++ FKA+HIAGTKG
Sbjct: 26  LKDFLNYLDNLKNYEKCGVPKDAGTDSSHGFDLGRMNRLMDRLGNPETGFKAVHIAGTKG 85

Query: 61  KGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFD 120
           KGSTAAFL+NILR EGY+VGCYTSPH+ TIRERISLG+ G+ VS KALN LF R K + D
Sbjct: 86  KGSTAAFLANILRTEGYAVGCYTSPHVRTIRERISLGKLGEPVSAKALNCLFHRIKPILD 145

Query: 121 QSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITS 180
           ++V LENG LSHFE+LTAMAF LFAQE+VDVAVIEAGLGGARDATNII SS LAAAVIT+
Sbjct: 146 EAVALENGRLSHFEILTAMAFKLFAQENVDVAVIEAGLGGARDATNIISSSGLAAAVITT 205

Query: 181 IGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASD 240
           IGEEH+AALGGSLESIA AK+GIIK+GCP +LGGPFLP IE+I  DKA SM SPV+SAS 
Sbjct: 206 IGEEHLAALGGSLESIAMAKSGIIKQGCPLVLGGPFLPHIEHIFLDKASSMCSPVVSASG 265

Query: 241 PGNRSTIKGVNLLNGGLSQCCDIVIQIDNE---FIELLDVNLRMLGPHQLQNAATATCVI 300
           PGNRS +KGV+  NG   Q CDIVI+++ +   FIEL DV L+MLG HQLQNAATATCV 
Sbjct: 266 PGNRSAVKGVSKSNGKPFQSCDIVIEVERDFKLFIELFDVKLQMLGIHQLQNAATATCVA 325

Query: 301 LTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKAL 360
           L LR+ GWRISD SI +GLE  +L+GRS FL + EAE LGLPGATI+LDGAHTK+SAKAL
Sbjct: 326 LCLRDKGWRISDESIHAGLEHAYLLGRSQFLTSTEAETLGLPGATIMLDGAHTKESAKAL 385

Query: 361 LDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAF 420
           +DTIQM FPEA+LAL+VAMASDK+H+ FAREFL GG+LE+V LTE  I G KSRTTSA+ 
Sbjct: 386 VDTIQMTFPEARLALIVAMASDKDHMAFAREFLSGGQLEAVFLTEVNIAGAKSRTTSASM 445

Query: 421 LRDCWIQASNELGI-PISLETKDAEVFF-------TSKLGNRPVLTTETSLLHAIKIAAE 480
           LRDCWIQAS ELGI  +    ++ +  F         +  ++ +L  E SLL ++++  +
Sbjct: 446 LRDCWIQASKELGINTLHDGMEEYQKLFENQSFCSAGESKHKTILAAENSLLVSLRVGNQ 505

Query: 481 ILKQRTKGRQGLVVVSGSLHAVSMVLASL 499
           IL+ RT+ +  ++V++GSLH VS VL+SL
Sbjct: 506 ILRARTRDQTSIIVITGSLHIVSTVLSSL 534

BLAST of Cucsa.012920 vs. TrEMBL
Match: M5X8R6_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa020012mg PE=4 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 6.4e-185
Identity = 338/499 (67.74%), Postives = 406/499 (81.36%), Query Frame = 1

Query: 5   MEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGST 64
           MEY++SLKNYEK GVP+ +GTDS+DGFDLGRMRRL+E LGNP S+FKA+HIAGTKGKGST
Sbjct: 1   MEYMNSLKNYEKSGVPKDAGTDSEDGFDLGRMRRLLELLGNPHSKFKAVHIAGTKGKGST 60

Query: 65  AAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVE 124
           AAFLS+ILR EG+SVGCYTSPHI+TIRERIS+GR G+ VS KAL SLF R K++ D+++E
Sbjct: 61  AAFLSSILRAEGHSVGCYTSPHIQTIRERISMGRFGEPVSAKALRSLFHRIKKILDRAME 120

Query: 125 LENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITSIGEE 184
           +ENG +SHFEVLTAMAF+LFAQE+V +AVIEAGLGGARDATN+ICSS LA +VIT+IGEE
Sbjct: 121 VENGRMSHFEVLTAMAFTLFAQENVGIAVIEAGLGGARDATNVICSSGLATSVITTIGEE 180

Query: 185 HVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNR 244
           H+AALGGSLESIATAK+GIIK G P +LGGPFLP IE+ILRDKA  MSSPV+ ASD GNR
Sbjct: 181 HLAALGGSLESIATAKSGIIKHGRPVVLGGPFLPHIEHILRDKAFLMSSPVVLASDAGNR 240

Query: 245 STIKGVNLLNGGLSQCCDIVIQIDNE---FIELLDVNLRMLGPHQLQNAATATCVILTLR 304
           S I GV++LNG   Q CDIVIQ++ +   FIELLDV L MLG HQLQNA TATC  L LR
Sbjct: 241 SKINGVSMLNGRPFQSCDIVIQLERDLKLFIELLDVKLYMLGTHQLQNAVTATCAALCLR 300

Query: 305 NLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDTI 364
           NLGWRISD  IR+GL+QT L+GRS FL ++EA+ + L  ATIL+DGAHTK+SA+AL++TI
Sbjct: 301 NLGWRISDECIRTGLQQTHLLGRSQFLTSKEAQAIRLSDATILVDGAHTKESARALMETI 360

Query: 365 QMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRDC 424
           +M FPEA L LVVAMASDK+H+GFARE L GG ++ VVLTEA I G KSRTT+A+ LR+C
Sbjct: 361 KMTFPEAGLTLVVAMASDKDHLGFARECLSGGHVDGVVLTEASIAGSKSRTTAASLLRNC 420

Query: 425 WIQASNELGIPI----SLETKDAEV---FFTSKLGNRPVLTTETSLLHAIKIAAEILKQR 484
           WIQAS ELGI +      E +D  V      SKLGN+P+L  E S   ++  A +IL+ R
Sbjct: 421 WIQASKELGIDVIHDKMSEHQDLSVDQLICLSKLGNKPILAAEASFPASLNTANQILRGR 480

Query: 485 TKGRQGLVVVSGSLHAVSM 494
              R G+VV++GSLH VS+
Sbjct: 481 AGDRLGIVVITGSLHIVSL 499

BLAST of Cucsa.012920 vs. TrEMBL
Match: A0A061G8S9_THECC (Folylpolyglutamate synthetase family protein isoform 2 OS=Theobroma cacao GN=TCM_015343 PE=4 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 4.1e-184
Identity = 336/510 (65.88%), Postives = 410/510 (80.39%), Query Frame = 1

Query: 1   MTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKG 60
           +  F++Y+DSLKNYEK GVP+ +GTDSDDGFDLGRMRRLM+RLGNPQS FK++HIAGTKG
Sbjct: 39  LKDFIQYIDSLKNYEKSGVPKDAGTDSDDGFDLGRMRRLMDRLGNPQSNFKSVHIAGTKG 98

Query: 61  KGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFD 120
           KGSTAA+LSNILR EGYSVGCYTSPHI +IRER+S+GR G  VS   LN LF R K+  D
Sbjct: 99  KGSTAAYLSNILRTEGYSVGCYTSPHILSIRERMSVGRLGKPVSSNTLNCLFHRIKQSLD 158

Query: 121 QSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITS 180
           +++ LENG LSHFEVLTA+AF+LFAQE+VD+A+IEAGLGGARDATNII SSELAA++IT+
Sbjct: 159 EAIILENGCLSHFEVLTAVAFTLFAQENVDIAIIEAGLGGARDATNIISSSELAASIITT 218

Query: 181 IGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASD 240
           IGEEH+AALGGSLESIA AKAGIIK G P ILGGPFLP I+ ILRDKALSMSSP++SASD
Sbjct: 219 IGEEHLAALGGSLESIAMAKAGIIKHGRPLILGGPFLPHIDCILRDKALSMSSPIVSASD 278

Query: 241 PGNRSTIKGVNLLNGGLSQCCDIVIQIDNEF---IELLDVNLRMLGPHQLQNAATATCVI 300
            G R+ IKGV+   G  SQ CD++IQ+D +F   IEL D+NL MLG HQLQNA TA C  
Sbjct: 279 SGIRTAIKGVSTFKGRPSQSCDLMIQLDCDFQLSIELCDLNLSMLGTHQLQNAVTAACTA 338

Query: 301 LTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKAL 360
           L L N GW+ISD SIR+GLE T L GRS FL ++EAE LGLPGAT+L+DGAHTK+SAKAL
Sbjct: 339 LCLCNQGWKISDGSIRAGLENTCLQGRSQFLTSKEAETLGLPGATVLIDGAHTKESAKAL 398

Query: 361 LDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAF 420
           LDTIQM FP+++LA+VVAMA DK+H+ FA+E L G ++E+V LTE+ I GG SRTTSA+ 
Sbjct: 399 LDTIQMTFPDSRLAIVVAMACDKDHLAFAKELLSGRQVEAVFLTESNIAGGTSRTTSASV 458

Query: 421 LRDCWIQASNELGIPISLET--------KDAEVFFTSKLGNRPVLTTETSLLHAIKIAAE 480
           LRDCW+QAS ELGI +  +         +D  +  T  L +  ++ TE SL  +++ A +
Sbjct: 459 LRDCWMQASRELGIKVLHDRIAEYRELFEDKYICSTRDLNHEILVATENSLSDSLRFANQ 518

Query: 481 ILKQRTKGRQGLVVVSGSLHAVSMVLASLH 500
           IL++RT  R G++VV+GSLH VS+VLASL+
Sbjct: 519 ILRERTWNRSGIIVVTGSLHIVSLVLASLN 548

BLAST of Cucsa.012920 vs. TrEMBL
Match: U5FM82_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0015s06150g PE=4 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 1.5e-181
Identity = 337/502 (67.13%), Postives = 411/502 (81.87%), Query Frame = 1

Query: 3   QFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKG 62
           +F++YLDSLKNYEKLGVP+ +GTDSDDG DLGRMRRLM+RLGNPQS+FKA+H+AGTKGKG
Sbjct: 17  EFIDYLDSLKNYEKLGVPKDAGTDSDDGLDLGRMRRLMDRLGNPQSKFKAVHVAGTKGKG 76

Query: 63  STAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQS 122
           STAA+LSNILR EGYSVGCYTSPH+ +IRERISLG+SG+ VS K LN LF   K   D++
Sbjct: 77  STAAYLSNILRAEGYSVGCYTSPHMMSIRERISLGQSGNPVSTKTLNKLFHMIKPKLDEA 136

Query: 123 VELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITSIG 182
           ++LENG L+HFEVLTA AF+L A+E VD+AVIEAGLGGARDATNI+CSSELAA+VIT+IG
Sbjct: 137 IQLENGSLTHFEVLTATAFTLMAEEKVDIAVIEAGLGGARDATNILCSSELAASVITTIG 196

Query: 183 EEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPG 242
           EEH+AALGGSLESIA AK+GIIK G P +LGGPFL  ++ ILRDKA  M SPV+SASD G
Sbjct: 197 EEHLAALGGSLESIAVAKSGIIKYGRPVVLGGPFLSHVDRILRDKASVMCSPVVSASDAG 256

Query: 243 NRSTIKGVNLLNGGLSQCCDIVIQIDNE---FIELLDVNLRMLGPHQLQNAATATCVILT 302
            R++IKG+ +L+G   Q  DI+IQ++ +   FIEL DV LRMLG HQL NA++A CV L 
Sbjct: 257 IRTSIKGLIILDGRPCQLSDIMIQVERDFPLFIELSDVKLRMLGRHQLHNASSAACVALC 316

Query: 303 LRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLD 362
           LR+ G RISD SIR+GLE TFL+GRS FL+++E EVLGLPGATILLDGAHTKDSAKAL+D
Sbjct: 317 LRDQGCRISDRSIRAGLENTFLLGRSQFLSSKETEVLGLPGATILLDGAHTKDSAKALVD 376

Query: 363 TIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLR 422
           T++MAFP+A++ALVVAMASDK+H+ FAREFL G +LE+V LTEA I GGKSRTTSA+ L 
Sbjct: 377 TVRMAFPDARVALVVAMASDKDHLAFAREFLSGLQLEAVFLTEADIAGGKSRTTSASLLM 436

Query: 423 DCWIQASNELGIPISLE--TKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKG 482
           DCWIQAS ELGI    +   K+ E+   +K+    +L TE S   A++ A E L++R   
Sbjct: 437 DCWIQASEELGINTLHDGMEKNRELLEENKI----ILATEKSPEVAMRAANETLRRRAGN 496

Query: 483 RQGLVVVSGSLHAVSMVLASLH 500
           R  ++VV+GSLH VS++LASLH
Sbjct: 497 RSSVIVVTGSLHIVSLLLASLH 514

BLAST of Cucsa.012920 vs. TAIR10
Match: AT5G41480.1 (AT5G41480.1 Folylpolyglutamate synthetase family protein)

HSP 1 Score: 552.0 bits (1421), Expect = 3.8e-157
Identity = 305/504 (60.52%), Postives = 378/504 (75.00%), Query Frame = 1

Query: 1   MTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKG 60
           +  F+ +L+SLKNYEK GVP+G+GTDSDDGFDLGRM+RLM RL NP  ++K +H+AGTKG
Sbjct: 45  LRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHVAGTKG 104

Query: 61  KGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFD 120
           KGST+AFLSNILR  GYSVGCY+SPHI +I+ERIS   +G+ VS   LN LF   K + +
Sbjct: 105 KGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSVKPILE 164

Query: 121 QSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITS 180
           QS++ ENG LSHFE+LT +AFSLF +E+VD+AVIEAGLGGARDATN+I SS LAA+VIT+
Sbjct: 165 QSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGARDATNVIESSNLAASVITT 224

Query: 181 IGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASD 240
           IGEEH+AALGGSLESIA AK+GIIK G P +LGGPFLP IE ILR KA S+SS VI AS+
Sbjct: 225 IGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIEGILRSKAASVSSSVILASN 284

Query: 241 PGNRSTIKGVNLLNG-GLSQCCDIVIQ---IDNEFIELLDVNLRMLGPHQLQNAATATCV 300
            G+ S+IKG+   NG GL Q CDIVIQ    D   +EL DVNLRMLG HQLQNA TATCV
Sbjct: 285 IGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDVNLRMLGHHQLQNAVTATCV 344

Query: 301 ILTLRNLG-WRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAK 360
            L LR+ G  R++D +IR GLE T L+GRS FL  +EAE L LPGAT+LLDGAHTK+SA+
Sbjct: 345 SLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAETLLLPGATVLLDGAHTKESAR 404

Query: 361 ALLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSA 420
           AL + I+  FPE +L  VVAMASDK+HV FA+E L G K E+V+LTEA IGGGK R+T +
Sbjct: 405 ALKEMIKKDFPEKRLVFVVAMASDKDHVSFAKELLSGLKPEAVILTEADIGGGKIRSTES 464

Query: 421 AFLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILK-QR 480
           + L++ WI+A++ELG   S+E  +                   ++L ++K+A +IL    
Sbjct: 465 SVLKESWIKAADELG-SRSMEASE-----------------NKTVLGSLKLAYKILSDDT 524

Query: 481 TKGRQGLVVVSGSLHAVSMVLASL 499
           T    G+V+V+GSLH VS VLASL
Sbjct: 525 TSSDSGMVIVTGSLHIVSSVLASL 528

BLAST of Cucsa.012920 vs. TAIR10
Match: AT3G55630.3 (AT3G55630.3 DHFS-FPGS homolog D)

HSP 1 Score: 117.1 bits (292), Expect = 3.2e-26
Identity = 100/314 (31.85%), Postives = 147/314 (46.82%), Query Frame = 1

Query: 48  SRFKAIHIAGTKGKGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKA 107
           S+ K IH+AGTKGKGST  F  +ILR  G   G +TSPH+  +RER  L  +G  +S + 
Sbjct: 56  SQMKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL--NGIEISQEK 115

Query: 108 LNSLFKRNKEVFDQSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNI 167
             + F        +    E    ++F  L  +AF +F  E VDV ++E GLGG  DATN+
Sbjct: 116 FVNYFWCCFHKLKEKTSNEVPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNV 175

Query: 168 ICSSELAAAVITSIGEEHVAALGGSLESIATAKAGIIKRGCPTI-LGGPFLPRIEYILRD 227
           I   +     I+S+G +H+  LG +L  IA  KAGI K G P   +  P       +L +
Sbjct: 176 I--QKPVVCGISSLGYDHMEILGYTLAEIAAEKAGIFKSGVPAFTVAQP--DEAMRVLNE 235

Query: 228 KA--LSMSSPVISASDPGNRSTIKGVN-LLNGGLSQCCDIVIQIDNEFIELLDVNLRMLG 287
           KA  L ++  V+   D   R  ++G +  LN GL+             + L    L+ +G
Sbjct: 236 KASKLEVNLQVVEPLDSSQRLGLQGEHQYLNAGLA-------------VALCSTFLKEIG 295

Query: 288 PHQLQNAATATCVILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATI 347
               +N    T  +                SGL   +L+GR+  +   E     LP   +
Sbjct: 296 IED-KNGLDQTNGL-----------PEKFISGLSNAYLMGRAMIVPDSE-----LPEEIV 333

Query: 348 -LLDGAHTKDSAKA 357
             LDGAH+ +S +A
Sbjct: 356 YYLDGAHSPESMEA 333

BLAST of Cucsa.012920 vs. TAIR10
Match: AT5G05980.1 (AT5G05980.1 DHFS-FPGS homolog B)

HSP 1 Score: 114.0 bits (284), Expect = 2.7e-25
Identity = 93/318 (29.25%), Postives = 145/318 (45.60%), Query Frame = 1

Query: 49  RFKAIHIAGTKGKGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKAL 108
           +   IH+AGTKGKGST  F  +I+R  G+  G +TSPH+  +RER  L    D+   K L
Sbjct: 111 KMNVIHVAGTKGKGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLD-GVDISEEKFL 170

Query: 109 NSL---FKRNKEVFDQSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDAT 168
                 + R KE  ++ + +     ++F  L  +AF +FA E+VD A++E GLGG  DAT
Sbjct: 171 GYFWWCYNRLKERTNEEIPMP----TYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDAT 230

Query: 169 NIICSSELAAAVITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILR 228
           N +   +     I+S+G +H+  LG +L  IA  KAGI K G P     P       +L 
Sbjct: 231 NAV--QKPVVCGISSLGYDHMEILGDTLGKIAGEKAGIFKLGVPAFT-VPQPDEAMRVLE 290

Query: 229 DKA------LSMSSPVISASDPGNRSTIKGVN-LLNGGLSQCCDIVIQIDNEFIELLDVN 288
           +KA      L +  P+ +    G +  + G +  +N GL+             + L  + 
Sbjct: 291 EKASETEVNLEVVQPLTARLLSGQKLGLDGEHQYVNAGLA-------------VSLASIW 350

Query: 289 LRMLGPHQLQNAATATCVILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGL 348
           L+ +G  ++ +    +            I       GL    L GR+  +  +  E    
Sbjct: 351 LQQIGKLEVPSRTQMS------------ILPEKFIKGLATASLQGRAQVVPDQYTESRTS 395

Query: 349 PGATILLDGAHTKDSAKA 357
                 LDGAH+ +S +A
Sbjct: 411 GDLVFYLDGAHSPESMEA 395

BLAST of Cucsa.012920 vs. TAIR10
Match: AT3G10160.1 (AT3G10160.1 DHFS-FPGS homolog C)

HSP 1 Score: 106.3 bits (264), Expect = 5.6e-23
Identity = 74/186 (39.78%), Postives = 103/186 (55.38%), Query Frame = 1

Query: 51  KAIHIAGTKGKGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNS 110
           K IH+AGTKGKGST  F   ILR  G+  G +TSPH+  +RER  +    D+   K L  
Sbjct: 132 KVIHVAGTKGKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRID-GLDISEEKFLQY 191

Query: 111 LFKRNKEVFDQSVE-LENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIIC 170
            ++  K + +++V+ L    L  F+ LT +AF +F  E VDVAVIE GLGG  D+TN+I 
Sbjct: 192 FWECWKLLKEKAVDGLTMPPL--FQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVI- 251

Query: 171 SSELAAAVITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKAL 230
             +     I S+G +H+  LG +L  IA  KAGI K   P     P L     +L+  A 
Sbjct: 252 -QKPVVCGIASLGMDHMDILGNTLADIAFHKAGIFKPQIPAFT-VPQLSEAMDVLQKTAN 311

Query: 231 SMSSPV 236
           ++  P+
Sbjct: 312 NLEVPL 311

BLAST of Cucsa.012920 vs. NCBI nr
Match: gi|449463470|ref|XP_004149457.1| (PREDICTED: probable dihydrofolate synthetase isoform X2 [Cucumis sativus])

HSP 1 Score: 966.1 bits (2496), Expect = 2.4e-278
Identity = 500/500 (100.00%), Postives = 500/500 (100.00%), Query Frame = 1

Query: 1   MTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKG 60
           MTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKG
Sbjct: 49  MTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKG 108

Query: 61  KGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFD 120
           KGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFD
Sbjct: 109 KGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFD 168

Query: 121 QSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITS 180
           QSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITS
Sbjct: 169 QSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITS 228

Query: 181 IGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASD 240
           IGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASD
Sbjct: 229 IGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASD 288

Query: 241 PGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTL 300
           PGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTL
Sbjct: 289 PGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTL 348

Query: 301 RNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDT 360
           RNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDT
Sbjct: 349 RNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDT 408

Query: 361 IQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRD 420
           IQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRD
Sbjct: 409 IQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRD 468

Query: 421 CWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQG 480
           CWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQG
Sbjct: 469 CWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQG 528

Query: 481 LVVVSGSLHAVSMVLASLHS 501
           LVVVSGSLHAVSMVLASLHS
Sbjct: 529 LVVVSGSLHAVSMVLASLHS 548

BLAST of Cucsa.012920 vs. NCBI nr
Match: gi|778680896|ref|XP_011651415.1| (PREDICTED: probable dihydrofolate synthetase isoform X1 [Cucumis sativus])

HSP 1 Score: 953.7 bits (2464), Expect = 1.2e-274
Identity = 493/493 (100.00%), Postives = 493/493 (100.00%), Query Frame = 1

Query: 1   MTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKG 60
           MTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKG
Sbjct: 49  MTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKG 108

Query: 61  KGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFD 120
           KGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFD
Sbjct: 109 KGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFD 168

Query: 121 QSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITS 180
           QSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITS
Sbjct: 169 QSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITS 228

Query: 181 IGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASD 240
           IGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASD
Sbjct: 229 IGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASD 288

Query: 241 PGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTL 300
           PGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTL
Sbjct: 289 PGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTL 348

Query: 301 RNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDT 360
           RNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDT
Sbjct: 349 RNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDT 408

Query: 361 IQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRD 420
           IQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRD
Sbjct: 409 IQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRD 468

Query: 421 CWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQG 480
           CWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQG
Sbjct: 469 CWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQG 528

Query: 481 LVVVSGSLHAVSM 494
           LVVVSGSLHAVSM
Sbjct: 529 LVVVSGSLHAVSM 541

BLAST of Cucsa.012920 vs. NCBI nr
Match: gi|659130858|ref|XP_008465388.1| (PREDICTED: dihydrofolate synthetase isoform X1 [Cucumis melo])

HSP 1 Score: 904.0 bits (2335), Expect = 1.1e-259
Identity = 466/500 (93.20%), Postives = 484/500 (96.80%), Query Frame = 1

Query: 1   MTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKG 60
           MT+FMEYLDSLKNYEKLGVPRG+GTDSDDGFDLGRMRRLMERLGNPQS+FKAIHIAGTKG
Sbjct: 49  MTEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKG 108

Query: 61  KGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFD 120
           KGS AAFLSNILR EGYSVGCYTSPHIET+RERISLGRSG+MVSGKALNSLFKRNKEVFD
Sbjct: 109 KGSIAAFLSNILRAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFD 168

Query: 121 QSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITS 180
           QSVELE+G +SHFEVLTAMAFSLFAQE+VDVAVIEAGLGGARDATNIICSS LAAAVITS
Sbjct: 169 QSVELEHGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITS 228

Query: 181 IGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASD 240
           IGEEH+AALGGSLESIA AKAGIIKRGCPTILGG FLPRIEYILRDKALSMSSPVI+ SD
Sbjct: 229 IGEEHMAALGGSLESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSD 288

Query: 241 PGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTL 300
           PGNRSTIKGVN+LNGGL QCCDIVIQIDNEF+ELLDVNLRMLG HQLQNAATATCVILTL
Sbjct: 289 PGNRSTIKGVNMLNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTL 348

Query: 301 RNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDT 360
           RNLGWRISDASIRSGLEQTFLIGRSHFLAA+EAEVLGLPGATILLDGAHTKDSAKAL+DT
Sbjct: 349 RNLGWRISDASIRSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDT 408

Query: 361 IQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRD 420
           IQMAFPEA+LALV+AMASDKNHV FAREFLQGGKLE VVLTEA IGGGKSRTTSAAFLRD
Sbjct: 409 IQMAFPEARLALVIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRD 468

Query: 421 CWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQG 480
           CWIQAS ELGIPISLETKDAEV FTSKL NRPVLTTETSLLHAIKIAAEILKQRTKG+Q 
Sbjct: 469 CWIQASIELGIPISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQS 528

Query: 481 LVVVSGSLHAVSMVLASLHS 501
           LVVVSGSLHAVSMVL+SLHS
Sbjct: 529 LVVVSGSLHAVSMVLSSLHS 548

BLAST of Cucsa.012920 vs. NCBI nr
Match: gi|1009122795|ref|XP_015878195.1| (PREDICTED: dihydrofolate synthetase [Ziziphus jujuba])

HSP 1 Score: 682.2 bits (1759), Expect = 7.0e-193
Identity = 353/510 (69.22%), Postives = 418/510 (81.96%), Query Frame = 1

Query: 1   MTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKG 60
           +  FMEYLD+LKNYEK GVP+ +GTDSD+GFDLGRMRRLME LGNPQS FKA+HIAG+KG
Sbjct: 48  LKDFMEYLDALKNYEKSGVPKSAGTDSDEGFDLGRMRRLMELLGNPQSNFKAVHIAGSKG 107

Query: 61  KGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFD 120
           KGSTAAFLS+ILR EGYSVGCYTSPHI+TIRERISLGR G+ V+ KALNSLF R K++ D
Sbjct: 108 KGSTAAFLSSILRAEGYSVGCYTSPHIQTIRERISLGRFGEPVAAKALNSLFNRTKKIID 167

Query: 121 QSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITS 180
           Q+VELENG +SHFEVLTAMAF+LFAQE+VD+AVIEAGLGGARDATN+I SS LA +VIT+
Sbjct: 168 QAVELENGCISHFEVLTAMAFTLFAQENVDIAVIEAGLGGARDATNVISSSGLALSVITT 227

Query: 181 IGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASD 240
           IGEEH+AALGGSLESIA AK+GIIK GCP +LGGPFLP IE ILR+KA SM SPVISA D
Sbjct: 228 IGEEHMAALGGSLESIAVAKSGIIKHGCPVVLGGPFLPHIECILRNKASSMHSPVISAYD 287

Query: 241 PGNRSTIKGVNLLNGGLSQCCDIVIQIDNE---FIELLDVNLRMLGPHQLQNAATATCVI 300
            GN+S IKG+++ NG   Q CDIVIQ+++E   FIEL DV L MLG HQLQNAATATC  
Sbjct: 288 TGNQSKIKGISIHNGRPCQSCDIVIQVESEINLFIELFDVQLYMLGSHQLQNAATATCAA 347

Query: 301 LTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKAL 360
           L LRNLGW+ISD SI+ GL+ T L+GRS FL ++EAE LG+    I+LDGAHTK+SAKAL
Sbjct: 348 LCLRNLGWKISDRSIKDGLQHTHLLGRSQFLTSKEAEALGVSKPMIMLDGAHTKESAKAL 407

Query: 361 LDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAF 420
           ++TIQM FP A+LALVVAMASDK+HVGFAREFL GG+LE V+LTEA I GGKSRT +A+F
Sbjct: 408 VETIQMTFPRARLALVVAMASDKDHVGFAREFLSGGQLEGVLLTEADIAGGKSRTAAASF 467

Query: 421 LRDCWIQASNELGIP-ISLETKDAEVFFTSKLG-------NRPVLTTETSLLHAIKIAAE 480
           LRDCWI+AS ELGI  +  +  D +  F  +L        NR +L  E S L ++KIA +
Sbjct: 468 LRDCWIKASEELGITLVHDKMSDYQELFNDQLDCSASTIQNRTILAVEASFLSSMKIADQ 527

Query: 481 ILKQRTKGRQGLVVVSGSLHAVSMVLASLH 500
           IL++RT    G++VV+GSLH VS+VLA+LH
Sbjct: 528 ILRKRTGNGFGIIVVTGSLHIVSLVLATLH 557

BLAST of Cucsa.012920 vs. NCBI nr
Match: gi|657969912|ref|XP_008376690.1| (PREDICTED: probable dihydrofolate synthetase [Malus domestica])

HSP 1 Score: 679.1 bits (1751), Expect = 5.9e-192
Identity = 348/505 (68.91%), Postives = 415/505 (82.18%), Query Frame = 1

Query: 5   MEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGST 64
           +EY+DSLKNYEKLGVP+ +GTDS+DGFDLGRMRRL+E LGNP S+FKA+HIAGTKGKGST
Sbjct: 50  LEYMDSLKNYEKLGVPKDAGTDSEDGFDLGRMRRLLELLGNPHSKFKAVHIAGTKGKGST 109

Query: 65  AAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVE 124
           AAFLSN+LR EGYSVGCYTSPHI+TIRERIS+GR  + VS KAL+SLF R K++ D ++E
Sbjct: 110 AAFLSNMLRAEGYSVGCYTSPHIQTIRERISVGRFAEPVSAKALSSLFDRVKKILDHAIE 169

Query: 125 LENGHLSHFEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITSIGEE 184
           +ENG  SHFEVLTA+AF+LFAQE+VD+AVIEAGLGGARDATN+ICSSELAA+VIT+IGEE
Sbjct: 170 VENGRTSHFEVLTALAFTLFAQENVDIAVIEAGLGGARDATNVICSSELAASVITTIGEE 229

Query: 185 HVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNR 244
           H+AALGGSLESIA AK+GIIK GCP +LGGPFLP IE ILRDKAL MSSPV+ ASD GN+
Sbjct: 230 HLAALGGSLESIAMAKSGIIKHGCPVVLGGPFLPHIEQILRDKALLMSSPVVLASDTGNK 289

Query: 245 STIKGVNLLNGGLSQCCDIVIQIDNE---FIELLDVNLRMLGPHQLQNAATATCVILTLR 304
           S I GV++LNG   Q CDIVI+++ +   FIELLDV L MLG HQLQNAATATC  L LR
Sbjct: 290 SKINGVSMLNGRPFQSCDIVIRVERDLKLFIELLDVKLYMLGTHQLQNAATATCAALCLR 349

Query: 305 NLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDTI 364
           NLGWRISD SIR+GLEQT L GRS FL ++EA+ LGL G TILLDGAHTK+SA+AL+DT+
Sbjct: 350 NLGWRISDGSIRTGLEQTRLFGRSQFLTSKEAQALGLSGTTILLDGAHTKESARALMDTV 409

Query: 365 QMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRDC 424
           +M FPEA L LVVAMASDK+H+GFA+E L GG +E VVLTEA I G  SRTT+A+ LRDC
Sbjct: 410 KMTFPEAGLTLVVAMASDKDHLGFAKECLSGGHVEGVVLTEANIAGATSRTTAASSLRDC 469

Query: 425 WIQASNEL-------GIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQR 484
           WIQAS EL        +P    +   ++   SK GN+P+L+TE S   ++KI  +I+K R
Sbjct: 470 WIQASQELETDILHDEMPEHQNSTMDQLLCISKSGNKPILSTEGSFQASLKITNQIIKGR 529

Query: 485 TKGRQGLVVVSGSLHAVSMVLASLH 500
           T  R  +VV++GSLH VS+VLA+LH
Sbjct: 530 TGDRLQIVVIAGSLHIVSLVLATLH 554