BLAST of Cp4.1LG14g06540 vs. Swiss-Prot
Match:
DHFS_ARATH (Dihydrofolate synthetase OS=Arabidopsis thaliana GN=DHFS PE=1 SV=1)
HSP 1 Score: 555.4 bits (1430), Expect = 6.7e-157
Identity = 305/509 (59.92%), Postives = 383/509 (75.25%), Query Frame = 1
Query: 44 SEDQQMTEFMEYLDSLKNYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHI 103
+ED ++ +F+ +L+SLKNYEK GVP GAGTDS+DGFDLGRMKRLM RL NP K+K +H+
Sbjct: 40 TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99
Query: 104 AGTKGKGSTAAFLSSILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRN 163
AGTKGKGST+AFLS+ILRA GYSVGCY+SPHI +I+ERIS +GE VS LN LF
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159
Query: 164 KELLDQSVKLENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAA 223
K +L+QS++ ENG +SHFE+LT +AFSLF +ENVD+AVIEAGLGGARDATN+I SS LAA
Sbjct: 160 KPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGARDATNVIESSNLAA 219
Query: 224 AVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPV 283
+VIT+IGEEHMAALGGSLESIA AK+GIIKHG P +LGGPF+P+IE ILR KA S+SS V
Sbjct: 220 SVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIEGILRSKAASVSSSV 279
Query: 284 VSASDPGNRSTIKGVSLLNG-RLCQCCDLIIQT---DNEFIELFDVNLRMLGRHQLQNAA 343
+ AS+ G+ S+IKG+ NG LCQ CD++IQ D +EL DVNLRMLG HQLQNA
Sbjct: 280 ILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDVNLRMLGHHQLQNAV 339
Query: 344 TATCVILTLRNLG-WRISDASIRSGLEKTFLVGRSHFLSAKEAGMLGLPGTTILLDGAHT 403
TATCV L LR+ G R++D +IR GLE T L+GRS FL+ KEA L LPG T+LLDGAHT
Sbjct: 340 TATCVSLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAETLLLPGATVLLDGAHT 399
Query: 404 KDSAKALVDTIQMSFPDAQLALVVAMASDKDHNGFATEFLQGGKLESIVLSEANIGGGKS 463
K+SA+AL + I+ FP+ +L VVAMASDKDH FA E L G K E+++L+EA+IGGGK
Sbjct: 400 KESARALKEMIKKDFPEKRLVFVVAMASDKDHVSFAKELLSGLKPEAVILTEADIGGGKI 459
Query: 464 RTTSAALLRDCWIQASNEMGIPISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEI 523
R+T +++L++ WI+A++E+G S EN+ V L ++K+A +I
Sbjct: 460 RSTESSVLKESWIKAADELG-----------SRSMEASENKTV-------LGSLKLAYKI 519
Query: 524 LK-QRIEGRRGLVVVTGSLHAVSMVLSSL 547
L G+V+VTGSLH VS VL+SL
Sbjct: 520 LSDDTTSSDSGMVIVTGSLHIVSSVLASL 528
BLAST of Cp4.1LG14g06540 vs. Swiss-Prot
Match:
FOLC_BACSU (Folylpolyglutamate synthase OS=Bacillus subtilis (strain 168) GN=folC PE=3 SV=2)
HSP 1 Score: 184.5 bits (467), Expect = 3.1e-45
Identity = 125/354 (35.31%), Postives = 190/354 (53.67%), Query Frame = 1
Query: 81 LGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSSILRAEGYSVGCYTSPHIETIRE 140
LGRMK+LM RLG+P+ K +A H+AGT GKGST AF+ S+L+ GY+VG +TSP+I T E
Sbjct: 24 LGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNE 83
Query: 141 RISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISHFEVLTAMAFSLFAQ-ENVDV 200
RIS+ +G +S + L + K ++ + E G+ + FE++TA AF FA+ VD
Sbjct: 84 RISV--NGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIMTACAFLYFAEFHKVDF 143
Query: 201 AVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTI 260
+ E GLGG D+TN++ VITSIG +HM LG ++E IA KAGIIK G P I
Sbjct: 144 VIFETGLGGRFDSTNVV---EPLLTVITSIGHDHMNILGNTIEEIAGEKAGIIKEGIP-I 203
Query: 261 LGGPFIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSLLNGRLCQCCD-LIIQTDNE 320
+ P ++R +A ++P S D + N + +T+ +
Sbjct: 204 VTAVTQPEALQVIRHEAERHAAPFQSLHD--------ACVIFNEEALPAGEQFSFKTEEK 263
Query: 321 FIELFDVNLRMLGRHQLQNAATATCVI--LTLRNLGWRISDASIRSGLEKTFLVGRSHFL 380
E D+ ++G HQ QNAA + L N+ ISD ++RSGL K GR +
Sbjct: 264 CYE--DIRTSLIGTHQRQNAALSILAAEWLNKENIA-HISDEALRSGLVKAAWPGRLELV 323
Query: 381 SAKEAGMLGLPGTTILLDGAHTKDSAKALVDTIQMSFPDAQLALVVAMASDKDH 431
+ LDGAH ++ + L +T++ F ++++++V + DK +
Sbjct: 324 QEH---------PPVYLDGAHNEEGVEKLAETMKQRFANSRISVVFSALKDKPY 351
BLAST of Cp4.1LG14g06540 vs. Swiss-Prot
Match:
FOLCP_HALVD (Probable bifunctional folylpolyglutamate synthase/dihydropteroate synthase OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) GN=folCP PE=3 SV=1)
HSP 1 Score: 139.0 bits (349), Expect = 1.5e-31
Identity = 112/375 (29.87%), Postives = 175/375 (46.67%), Query Frame = 1
Query: 84 MKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSSILRAEGYSVGCYTSPHIETIRERIS 143
++RL+ LG+P + +AG+ GKGSTA + ++LR G VG YTSPH + +RER+
Sbjct: 25 IRRLLSHLGDPHEGVSFVQVAGSNGKGSTARMVDAMLRESGAHVGLYTSPHFDDVRERVR 84
Query: 144 LGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISHFEVLTAMAFSLFAQENVDVAVIE 203
+ G + AL+ K L + + ++ FE +TA+A F + VDVAV+E
Sbjct: 85 V--DGRKIPKSALSAFVAEAKPYLVERA-ADGEPLTFFETVTALALWYFDRAGVDVAVLE 144
Query: 204 AGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGP 263
G+GG DAT+ + A+ +T++ EH A LG ++ IA KA + P + G
Sbjct: 145 VGMGGELDATSAVDP---VASAVTNVSLEHTAVLGDTVAEIAKTKAAVAPADAPLVTG-- 204
Query: 264 FIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSLLNGRL-CQCCDLIIQTDNEFIEL 323
++R++A V++ D S GR+ Q + ++TD E
Sbjct: 205 TTGEALSVIREEA----GDVLTVGDADADSDADVRVSYGGRVNHQEAAVTVETD---AET 264
Query: 324 FDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASIRSGLEKTFLVGRSHFLSAKEAG 383
DV + +LG +Q +NA A + +R I +I GL GR + +
Sbjct: 265 LDVRIPLLGAYQARNAGIAVSLARQVRP---DIDAEAIHRGLRNAHWPGRFEVMGTE--- 324
Query: 384 MLGLPGTTILLDGAHTKDSAKALVDTIQMSFPDAQLALVVAMASDKDHNGFATEFLQGGK 443
T++LDGAH D A A V T+ F L LV DKDH +
Sbjct: 325 ------PTVVLDGAHNPD-ACAQVATVLDEFDYDDLHLVYGAMHDKDHGEMVGAL---PE 368
Query: 444 LESIVLSEANIGGGK 458
+ S+V +A+I G+
Sbjct: 385 VASVVTCKADISRGE 368
BLAST of Cp4.1LG14g06540 vs. Swiss-Prot
Match:
FOLC_LACCA (Folylpolyglutamate synthase OS=Lactobacillus casei GN=fgs PE=1 SV=1)
HSP 1 Score: 137.9 bits (346), Expect = 3.4e-31
Identity = 107/388 (27.58%), Postives = 174/388 (44.85%), Query Frame = 1
Query: 50 TEFMEYLDSLKNYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGK 109
TE + Y+ S K G D R+ L+ LGNPQ + + IH+ GT GK
Sbjct: 4 TETVAYIHSFPRLAKTG-------------DHRRILTLLHALGNPQQQGRYIHVTGTNGK 63
Query: 110 GSTAAFLSSILRAEGYSVGCYTSPHIETIRERISLGRS--GEMVSGKALNFLFKRNKELL 169
GS A ++ +L A G +VG YTSP I ERI + + A+ F+ + L
Sbjct: 64 GSAANAIAHVLEASGLTVGLYTSPFIMRFNERIMIDHEPIPDAALVNAVAFVRAALERLQ 123
Query: 170 DQSVKLENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVIT 229
Q ++ FE +TA+ + F Q VDVAVIE G+GG D+TN+I +V+T
Sbjct: 124 QQQADF---NVTEFEFITALGYWYFRQRQVDVAVIEVGIGGDTDSTNVITP---VVSVLT 183
Query: 230 SIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSAS 289
+ +H LG ++ +IA KAGIIK G P + G +P+ ++ K + S +
Sbjct: 184 EVALDHQKLLGHTITAIAKHKAGIIKRGIPVVTGN-LVPDAAAVVAAKVATTGSQWLRFD 243
Query: 290 DPGNRSTIKGVSLLNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATA-TCVIL 349
+ S+ +L D + + D+ + ++G +Q +N A A +
Sbjct: 244 --------RDFSVPKAKLHGWGQRFTYEDQDG-RISDLEVPLVGDYQQRNMAIAIQTAKV 303
Query: 350 TLRNLGWRISDASIRSGLEKTFLVGRSHFLSAKEAGMLGLPGTTILLDGAHTKDSAKALV 409
+ W ++ +IR GL + R +S I++DGAH D L+
Sbjct: 304 YAKQTEWPLTPQNIRQGLAASHWPARLEKIS---------DTPLIVIDGAHNPDGINGLI 352
Query: 410 DTIQMSFPDAQLALVVAMASDKDHNGFA 435
++ F + ++ + +DKD+ A
Sbjct: 364 TALKQLF-SQPITVIAGILADKDYAAMA 352
BLAST of Cp4.1LG14g06540 vs. Swiss-Prot
Match:
FOLD_SCHPO (Probable dihydrofolate synthetase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=fol3 PE=3 SV=1)
HSP 1 Score: 136.0 bits (341), Expect = 1.3e-30
Identity = 120/364 (32.97%), Postives = 175/364 (48.08%), Query Frame = 1
Query: 81 LGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSSILRAEGYSVGCYTSPHIETIRE 140
L RM +L++ LGNPQ F A+ IAGT GKGS +++ + L G YTSPH R+
Sbjct: 7 LQRMLQLLKHLGNPQESFCAVQIAGTNGKGSICSYIYTSLLQAAIKTGRYTSPHFLEPRD 66
Query: 141 RISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISHFEVLTAMAFSLFAQENVDVA 200
IS+ +G++ S + N +K+ E +D+ + + + FE+LTA AF F V VA
Sbjct: 67 TISI--NGQIASEEIFNTCWKQVIE-VDRRFRT---KATEFELLTATAFQCFHHSGVRVA 126
Query: 201 VIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTIL 260
VIE G+GG DATN+ ++I+ I +H A LG +LE+IA KAGI K P ++
Sbjct: 127 VIETGMGGRLDATNVF--EEPVLSIISRICLDHQAFLGNTLEAIAKEKAGIFKKNVPCVV 186
Query: 261 GGPFIPNIECILRDKA-LSMSSPVVSASDPGNRSTIKGVSLLNGRLCQCCDLIIQTDNEF 320
G N+ L+ A + + P A KG S N + II T N
Sbjct: 187 DGLNEVNVLNQLKLSAEETRAHPFYLA---------KGKSGENKN-----EWIINTPNWG 246
Query: 321 IELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASIRSGLEKTFLVGRSHFLSAK 380
F L+ G +Q QN A A L + + + I +++G++ T GR S
Sbjct: 247 TNTFSTPLK--GDYQGQNLACAV-TALDILSSSFSIMLPHVQNGVKNTSWPGRLDIRSVP 306
Query: 381 EAGMLGLPGTTILLDGAHTKDSAKALVDTI--QMSFPDAQLALVVAMASDKDHNGFATEF 440
G IL DGAH K++A L + Q + ++ VVA + KD G
Sbjct: 307 SLG-------DILFDGAHNKEAAIELAKFVNSQRREHNKSVSWVVAFTNTKDVTGIMKIL 338
Query: 441 LQGG 442
L+ G
Sbjct: 367 LRKG 338
BLAST of Cp4.1LG14g06540 vs. TrEMBL
Match:
A0A0A0L7A4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G354540 PE=4 SV=1)
HSP 1 Score: 933.3 bits (2411), Expect = 1.3e-268
Identity = 477/548 (87.04%), Postives = 513/548 (93.61%), Query Frame = 1
Query: 1 MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQQMTEFMEYLDSLK 60
MN+FKYHHH RPQI G LLLN+F+GE PSISSR GSKQCF HSEDQ MT+FMEYLDSLK
Sbjct: 1 MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60
Query: 61 NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSSIL 120
NYEKLGVP G+GTDS+DGFDLGRM+RLMERLGNPQS+FKAIHIAGTKGKGSTAAFLS+IL
Sbjct: 61 NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120
Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
R EGYSVGCYTSPHIETIRERISLGRSG+MVSGKALN LFKRNKE+ DQSV+LENG +SH
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180
Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
FEVLTAMAFSLFAQE+VDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEH+AALGGS
Sbjct: 181 FEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGS 240
Query: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSL 300
LESIA AKAGIIK GCPTILGGPF+P IE ILRDKALSMSSPV+SASDPGNRSTIKGV+L
Sbjct: 241 LESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNL 300
Query: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360
LNG L QCCD++IQ DNEFIEL DVNLRMLG HQLQNAATATCVILTLRNLGWRISDASI
Sbjct: 301 LNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTLRNLGWRISDASI 360
Query: 361 RSGLEKTFLVGRSHFLSAKEAGMLGLPGTTILLDGAHTKDSAKALVDTIQMSFPDAQLAL 420
RSGLE+TFL+GRSHFL+A+EA +LGLPG TILLDGAHTKDSAKAL+DTIQM+FP+AQLAL
Sbjct: 361 RSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLAL 420
Query: 421 VVAMASDKDHNGFATEFLQGGKLESIVLSEANIGGGKSRTTSAALLRDCWIQASNEMGIP 480
VVAMASDK+H GFA EFLQGGKLES+VL+EA IGGGKSRTTSAA LRDCWIQASNE+GIP
Sbjct: 421 VVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRDCWIQASNELGIP 480
Query: 481 ISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQRIEGRRGLVVVTGSLHAVS 540
ISLETKDA V TSKL NRPVLTTETSLL AIKIAAEILKQR +GR+GLVVV+GSLHAVS
Sbjct: 481 ISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQGLVVVSGSLHAVS 540
Query: 541 MVLSSLHS 549
MVL+SLHS
Sbjct: 541 MVLASLHS 548
BLAST of Cp4.1LG14g06540 vs. TrEMBL
Match:
A0A061G8S9_THECC (Folylpolyglutamate synthetase family protein isoform 2 OS=Theobroma cacao GN=TCM_015343 PE=4 SV=1)
HSP 1 Score: 668.7 bits (1724), Expect = 6.1e-189
Identity = 343/526 (65.21%), Postives = 425/526 (80.80%), Query Frame = 1
Query: 33 RIGSKQCFGNHSEDQQMTEFMEYLDSLKNYEKLGVPTGAGTDSEDGFDLGRMKRLMERLG 92
R+ SKQ F ++E+ ++ +F++Y+DSLKNYEK GVP AGTDS+DGFDLGRM+RLM+RLG
Sbjct: 23 RLESKQWFSTYTEEPELKDFIQYIDSLKNYEKSGVPKDAGTDSDDGFDLGRMRRLMDRLG 82
Query: 93 NPQSKFKAIHIAGTKGKGSTAAFLSSILRAEGYSVGCYTSPHIETIRERISLGRSGEMVS 152
NPQS FK++HIAGTKGKGSTAA+LS+ILR EGYSVGCYTSPHI +IRER+S+GR G+ VS
Sbjct: 83 NPQSNFKSVHIAGTKGKGSTAAYLSNILRTEGYSVGCYTSPHILSIRERMSVGRLGKPVS 142
Query: 153 GKALNFLFKRNKELLDQSVKLENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDA 212
LN LF R K+ LD+++ LENG +SHFEVLTA+AF+LFAQENVD+A+IEAGLGGARDA
Sbjct: 143 SNTLNCLFHRIKQSLDEAIILENGCLSHFEVLTAVAFTLFAQENVDIAIIEAGLGGARDA 202
Query: 213 TNIICSSRLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFIPNIECIL 272
TNII SS LAA++IT+IGEEH+AALGGSLESIAMAKAGIIKHG P ILGGPF+P+I+CIL
Sbjct: 203 TNIISSSELAASIITTIGEEHLAALGGSLESIAMAKAGIIKHGRPLILGGPFLPHIDCIL 262
Query: 273 RDKALSMSSPVVSASDPGNRSTIKGVSLLNGRLCQCCDLIIQTDNEF---IELFDVNLRM 332
RDKALSMSSP+VSASD G R+ IKGVS GR Q CDL+IQ D +F IEL D+NL M
Sbjct: 263 RDKALSMSSPIVSASDSGIRTAIKGVSTFKGRPSQSCDLMIQLDCDFQLSIELCDLNLSM 322
Query: 333 LGRHQLQNAATATCVILTLRNLGWRISDASIRSGLEKTFLVGRSHFLSAKEAGMLGLPGT 392
LG HQLQNA TA C L L N GW+ISD SIR+GLE T L GRS FL++KEA LGLPG
Sbjct: 323 LGTHQLQNAVTAACTALCLCNQGWKISDGSIRAGLENTCLQGRSQFLTSKEAETLGLPGA 382
Query: 393 TILLDGAHTKDSAKALVDTIQMSFPDAQLALVVAMASDKDHNGFATEFLQGGKLESIVLS 452
T+L+DGAHTK+SAKAL+DTIQM+FPD++LA+VVAMA DKDH FA E L G ++E++ L+
Sbjct: 383 TVLIDGAHTKESAKALLDTIQMTFPDSRLAIVVAMACDKDHLAFAKELLSGRQVEAVFLT 442
Query: 453 EANIGGGKSRTTSAALLRDCWIQASNEMGIPISLET--------KDAPVSSTSKLENRPV 512
E+NI GG SRTTSA++LRDCW+QAS E+GI + + +D + ST L + +
Sbjct: 443 ESNIAGGTSRTTSASVLRDCWMQASRELGIKVLHDRIAEYRELFEDKYICSTRDLNHEIL 502
Query: 513 LTTETSLLRAIKIAAEILKQRIEGRRGLVVVTGSLHAVSMVLSSLH 548
+ TE SL +++ A +IL++R R G++VVTGSLH VS+VL+SL+
Sbjct: 503 VATENSLSDSLRFANQILRERTWNRSGIIVVTGSLHIVSLVLASLN 548
BLAST of Cp4.1LG14g06540 vs. TrEMBL
Match:
E0CUK0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0050g02490 PE=4 SV=1)
HSP 1 Score: 665.6 bits (1716), Expect = 5.1e-188
Identity = 345/524 (65.84%), Postives = 418/524 (79.77%), Query Frame = 1
Query: 34 IGSKQCFGNHSEDQQMTEFMEYLDSLKNYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGN 93
+G K+ F S + ++ +F+ YLD+LKNYEK GVP AGTDS GFDLGRM RLM+RLGN
Sbjct: 14 LGFKRSF---STEPELKDFLNYLDNLKNYEKCGVPKDAGTDSSHGFDLGRMNRLMDRLGN 73
Query: 94 PQSKFKAIHIAGTKGKGSTAAFLSSILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSG 153
P++ FKA+HIAGTKGKGSTAAFL++ILR EGY+VGCYTSPH+ TIRERISLG+ GE VS
Sbjct: 74 PETGFKAVHIAGTKGKGSTAAFLANILRTEGYAVGCYTSPHVRTIRERISLGKLGEPVSA 133
Query: 154 KALNFLFKRNKELLDQSVKLENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDAT 213
KALN LF R K +LD++V LENGR+SHFE+LTAMAF LFAQENVDVAVIEAGLGGARDAT
Sbjct: 134 KALNCLFHRIKPILDEAVALENGRLSHFEILTAMAFKLFAQENVDVAVIEAGLGGARDAT 193
Query: 214 NIICSSRLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFIPNIECILR 273
NII SS LAAAVIT+IGEEH+AALGGSLESIAMAK+GIIK GCP +LGGPF+P+IE I
Sbjct: 194 NIISSSGLAAAVITTIGEEHLAALGGSLESIAMAKSGIIKQGCPLVLGGPFLPHIEHIFL 253
Query: 274 DKALSMSSPVVSASDPGNRSTIKGVSLLNGRLCQCCDLIIQTDNE---FIELFDVNLRML 333
DKA SM SPVVSAS PGNRS +KGVS NG+ Q CD++I+ + + FIELFDV L+ML
Sbjct: 254 DKASSMCSPVVSASGPGNRSAVKGVSKSNGKPFQSCDIVIEVERDFKLFIELFDVKLQML 313
Query: 334 GRHQLQNAATATCVILTLRNLGWRISDASIRSGLEKTFLVGRSHFLSAKEAGMLGLPGTT 393
G HQLQNAATATCV L LR+ GWRISD SI +GLE +L+GRS FL++ EA LGLPG T
Sbjct: 314 GIHQLQNAATATCVALCLRDKGWRISDESIHAGLEHAYLLGRSQFLTSTEAETLGLPGAT 373
Query: 394 ILLDGAHTKDSAKALVDTIQMSFPDAQLALVVAMASDKDHNGFATEFLQGGKLESIVLSE 453
I+LDGAHTK+SAKALVDTIQM+FP+A+LAL+VAMASDKDH FA EFL GG+LE++ L+E
Sbjct: 374 IMLDGAHTKESAKALVDTIQMTFPEARLALIVAMASDKDHMAFAREFLSGGQLEAVFLTE 433
Query: 454 ANIGGGKSRTTSAALLRDCWIQASNEMGIPISLE--------TKDAPVSSTSKLENRPVL 513
NI G KSRTTSA++LRDCWIQAS E+GI + ++ S + +++ +L
Sbjct: 434 VNIAGAKSRTTSASMLRDCWIQASKELGINTLHDGMEEYQKLFENQSFCSAGESKHKTIL 493
Query: 514 TTETSLLRAIKIAAEILKQRIEGRRGLVVVTGSLHAVSMVLSSL 547
E SLL ++++ +IL+ R + ++V+TGSLH VS VLSSL
Sbjct: 494 AAENSLLVSLRVGNQILRARTRDQTSIIVITGSLHIVSTVLSSL 534
BLAST of Cp4.1LG14g06540 vs. TrEMBL
Match:
A0A0D2Q2P9_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_008G270800 PE=4 SV=1)
HSP 1 Score: 651.0 bits (1678), Expect = 1.3e-183
Identity = 333/531 (62.71%), Postives = 422/531 (79.47%), Query Frame = 1
Query: 28 PSISSRIGSKQCFGNHSEDQQMTEFMEYLDSLKNYEKLGVPTGAGTDSEDGFDLGRMKRL 87
P + ++ SKQC +++E+ ++ F +++DSLKNYEK GVP AGTDS+DGFDLGRMKRL
Sbjct: 19 PLSNFQLESKQCLSSYTEEPELEGFSQFIDSLKNYEKSGVPKDAGTDSDDGFDLGRMKRL 78
Query: 88 MERLGNPQSKFKAIHIAGTKGKGSTAAFLSSILRAEGYSVGCYTSPHIETIRERISLGRS 147
M RLGNP S FK++HIAGTKGKGSTAA+LS+ILR+EGYSVGCYTSPH+ +IRER+S+G+
Sbjct: 79 MTRLGNPLSNFKSVHIAGTKGKGSTAAYLSNILRSEGYSVGCYTSPHMLSIRERMSVGKM 138
Query: 148 GEMVSGKALNFLFKRNKELLDQSVKLENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLG 207
G+ VS ALN LF K L++++ LENG +SHFEVLTA+AF+LFAQENVD+A+IEAGLG
Sbjct: 139 GKPVSSNALNCLFHSIKRSLNEAIVLENGCLSHFEVLTAVAFALFAQENVDIAIIEAGLG 198
Query: 208 GARDATNIICSSRLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFIPN 267
GARDATN+I SS L A++IT+IGEEH+AALGGSLESIAMAKAGIIKHG P ILGGPF+P+
Sbjct: 199 GARDATNVISSSELDASIITTIGEEHLAALGGSLESIAMAKAGIIKHGRPVILGGPFLPH 258
Query: 268 IECILRDKALSMSSPVVSASDPGNRSTIKGVSLLNGRLCQCCDLIIQTDN---EFIELFD 327
I+ ILRD+A SM SP+VSASD G R++IKG+ + GR QCCDL+I+ D+ IEL D
Sbjct: 259 IDRILRDRAASMFSPIVSASDAGVRTSIKGIGMFKGRPSQCCDLVIELDHGSQSSIELRD 318
Query: 328 VNLRMLGRHQLQNAATATCVILTLRNLGWRISDASIRSGLEKTFLVGRSHFLSAKEAGML 387
+NL MLG HQLQNA TATC L LRN GWRIS+ SIR+GLE TFL GRS FLS+KEA L
Sbjct: 319 LNLSMLGTHQLQNAVTATCAALCLRNQGWRISNGSIRAGLENTFLPGRSQFLSSKEAEKL 378
Query: 388 GLPGTTILLDGAHTKDSAKALVDTIQMSFPDAQLALVVAMASDKDHNGFATEFLQGGKLE 447
GL G+T+L+DGAHTKDSAKAL++TIQ +FPD++LA+VVAMASDKDH FA EFL G +LE
Sbjct: 379 GLSGSTVLVDGAHTKDSAKALLETIQTTFPDSRLAIVVAMASDKDHLAFAKEFLSGKQLE 438
Query: 448 SIVLSEANIGGGKSRTTSAALLRDCWIQASNEMGIPISLET--------KDAPVSSTSKL 507
++ L+EA+I GG SRTTSA LRDCWIQAS E+GI + + +D +SST
Sbjct: 439 AVFLTEADIAGGTSRTTSATALRDCWIQASRELGIEVLHDRMTRYRELFEDNFISSTRDS 498
Query: 508 ENRPVLTTETSLLRAIKIAAEILKQRIEGRRGLVVVTGSLHAVSMVLSSLH 548
++ ++ + SL +++ A +IL++R G++VVTGSLH VS+VL+SL+
Sbjct: 499 KHETIVAAQNSLSDSLRFANQILRERTRNELGILVVTGSLHIVSLVLASLN 549
BLAST of Cp4.1LG14g06540 vs. TrEMBL
Match:
U5FM82_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0015s06150g PE=4 SV=1)
HSP 1 Score: 650.2 bits (1676), Expect = 2.2e-183
Identity = 340/513 (66.28%), Postives = 418/513 (81.48%), Query Frame = 1
Query: 40 FGNHSEDQQMTEFMEYLDSLKNYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFK 99
F ++E+ + EF++YLDSLKNYEKLGVP AGTDS+DG DLGRM+RLM+RLGNPQSKFK
Sbjct: 6 FSKYTEEPEPKEFIDYLDSLKNYEKLGVPKDAGTDSDDGLDLGRMRRLMDRLGNPQSKFK 65
Query: 100 AIHIAGTKGKGSTAAFLSSILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFL 159
A+H+AGTKGKGSTAA+LS+ILRAEGYSVGCYTSPH+ +IRERISLG+SG VS K LN L
Sbjct: 66 AVHVAGTKGKGSTAAYLSNILRAEGYSVGCYTSPHMMSIRERISLGQSGNPVSTKTLNKL 125
Query: 160 FKRNKELLDQSVKLENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSS 219
F K LD++++LENG ++HFEVLTA AF+L A+E VD+AVIEAGLGGARDATNI+CSS
Sbjct: 126 FHMIKPKLDEAIQLENGSLTHFEVLTATAFTLMAEEKVDIAVIEAGLGGARDATNILCSS 185
Query: 220 RLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSM 279
LAA+VIT+IGEEH+AALGGSLESIA+AK+GIIK+G P +LGGPF+ +++ ILRDKA M
Sbjct: 186 ELAASVITTIGEEHLAALGGSLESIAVAKSGIIKYGRPVVLGGPFLSHVDRILRDKASVM 245
Query: 280 SSPVVSASDPGNRSTIKGVSLLNGRLCQCCDLIIQTDNE---FIELFDVNLRMLGRHQLQ 339
SPVVSASD G R++IKG+ +L+GR CQ D++IQ + + FIEL DV LRMLGRHQL
Sbjct: 246 CSPVVSASDAGIRTSIKGLIILDGRPCQLSDIMIQVERDFPLFIELSDVKLRMLGRHQLH 305
Query: 340 NAATATCVILTLRNLGWRISDASIRSGLEKTFLVGRSHFLSAKEAGMLGLPGTTILLDGA 399
NA++A CV L LR+ G RISD SIR+GLE TFL+GRS FLS+KE +LGLPG TILLDGA
Sbjct: 306 NASSAACVALCLRDQGCRISDRSIRAGLENTFLLGRSQFLSSKETEVLGLPGATILLDGA 365
Query: 400 HTKDSAKALVDTIQMSFPDAQLALVVAMASDKDHNGFATEFLQGGKLESIVLSEANIGGG 459
HTKDSAKALVDT++M+FPDA++ALVVAMASDKDH FA EFL G +LE++ L+EA+I GG
Sbjct: 366 HTKDSAKALVDTVRMAFPDARVALVVAMASDKDHLAFAREFLSGLQLEAVFLTEADIAGG 425
Query: 460 KSRTTSAALLRDCWIQASNEMGIPISLETKDAPVSSTSKL--ENRPVLTTETSLLRAIKI 519
KSRTTSA+LL DCWIQAS E+GI T + +L EN+ +L TE S A++
Sbjct: 426 KSRTTSASLLMDCWIQASEELGI----NTLHDGMEKNRELLEENKIILATEKSPEVAMRA 485
Query: 520 AAEILKQRIEGRRGLVVVTGSLHAVSMVLSSLH 548
A E L++R R ++VVTGSLH VS++L+SLH
Sbjct: 486 ANETLRRRAGNRSSVIVVTGSLHIVSLLLASLH 514
BLAST of Cp4.1LG14g06540 vs. TAIR10
Match:
AT5G41480.1 (AT5G41480.1 Folylpolyglutamate synthetase family protein)
HSP 1 Score: 555.4 bits (1430), Expect = 3.8e-158
Identity = 305/509 (59.92%), Postives = 383/509 (75.25%), Query Frame = 1
Query: 44 SEDQQMTEFMEYLDSLKNYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHI 103
+ED ++ +F+ +L+SLKNYEK GVP GAGTDS+DGFDLGRMKRLM RL NP K+K +H+
Sbjct: 40 TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99
Query: 104 AGTKGKGSTAAFLSSILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRN 163
AGTKGKGST+AFLS+ILRA GYSVGCY+SPHI +I+ERIS +GE VS LN LF
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159
Query: 164 KELLDQSVKLENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAA 223
K +L+QS++ ENG +SHFE+LT +AFSLF +ENVD+AVIEAGLGGARDATN+I SS LAA
Sbjct: 160 KPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGARDATNVIESSNLAA 219
Query: 224 AVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPV 283
+VIT+IGEEHMAALGGSLESIA AK+GIIKHG P +LGGPF+P+IE ILR KA S+SS V
Sbjct: 220 SVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIEGILRSKAASVSSSV 279
Query: 284 VSASDPGNRSTIKGVSLLNG-RLCQCCDLIIQT---DNEFIELFDVNLRMLGRHQLQNAA 343
+ AS+ G+ S+IKG+ NG LCQ CD++IQ D +EL DVNLRMLG HQLQNA
Sbjct: 280 ILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDVNLRMLGHHQLQNAV 339
Query: 344 TATCVILTLRNLG-WRISDASIRSGLEKTFLVGRSHFLSAKEAGMLGLPGTTILLDGAHT 403
TATCV L LR+ G R++D +IR GLE T L+GRS FL+ KEA L LPG T+LLDGAHT
Sbjct: 340 TATCVSLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAETLLLPGATVLLDGAHT 399
Query: 404 KDSAKALVDTIQMSFPDAQLALVVAMASDKDHNGFATEFLQGGKLESIVLSEANIGGGKS 463
K+SA+AL + I+ FP+ +L VVAMASDKDH FA E L G K E+++L+EA+IGGGK
Sbjct: 400 KESARALKEMIKKDFPEKRLVFVVAMASDKDHVSFAKELLSGLKPEAVILTEADIGGGKI 459
Query: 464 RTTSAALLRDCWIQASNEMGIPISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEI 523
R+T +++L++ WI+A++E+G S EN+ V L ++K+A +I
Sbjct: 460 RSTESSVLKESWIKAADELG-----------SRSMEASENKTV-------LGSLKLAYKI 519
Query: 524 LK-QRIEGRRGLVVVTGSLHAVSMVLSSL 547
L G+V+VTGSLH VS VL+SL
Sbjct: 520 LSDDTTSSDSGMVIVTGSLHIVSSVLASL 528
BLAST of Cp4.1LG14g06540 vs. TAIR10
Match:
AT3G55630.3 (AT3G55630.3 DHFS-FPGS homolog D)
HSP 1 Score: 119.8 bits (299), Expect = 5.3e-27
Identity = 105/317 (33.12%), Postives = 147/317 (46.37%), Query Frame = 1
Query: 96 SKFKAIHIAGTKGKGSTAAFLSSILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKA 155
S+ K IH+AGTKGKGST F SILR G G +TSPH+ +RER L E+ K
Sbjct: 56 SQMKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL-NGIEISQEKF 115
Query: 156 LNFL---FKRNKELLDQSVKLENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDA 215
+N+ F + KE V + ++F L +AF +F E VDV ++E GLGG DA
Sbjct: 116 VNYFWCCFHKLKEKTSNEVPMP----TYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDA 175
Query: 216 TNIICSSRLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTI-LGGPFIPNIECI 275
TN+I + I+S+G +HM LG +L IA KAGI K G P + P +
Sbjct: 176 TNVI--QKPVVCGISSLGYDHMEILGYTLAEIAAEKAGIFKSGVPAFTVAQP--DEAMRV 235
Query: 276 LRDKA--LSMSSPVVSASDPGNRSTIKGV-SLLNGRLCQCCDLIIQTDNEFIELFDVNLR 335
L +KA L ++ VV D R ++G LN L + L L+
Sbjct: 236 LNEKASKLEVNLQVVEPLDSSQRLGLQGEHQYLNAGLA-------------VALCSTFLK 295
Query: 336 MLGRHQLQNAATATCVILTLRNLGWRISDASIRSGLEKTFLVGRSHFLSAKEAGMLGLPG 395
+G +N T + SGL +L+GR+ + E LP
Sbjct: 296 EIGIED-KNGLDQTNGL-----------PEKFISGLSNAYLMGRAMIVPDSE-----LPE 333
Query: 396 TTI-LLDGAHTKDSAKA 405
+ LDGAH+ +S +A
Sbjct: 356 EIVYYLDGAHSPESMEA 333
BLAST of Cp4.1LG14g06540 vs. TAIR10
Match:
AT5G05980.1 (AT5G05980.1 DHFS-FPGS homolog B)
HSP 1 Score: 115.2 bits (287), Expect = 1.3e-25
Identity = 92/317 (29.02%), Postives = 146/317 (46.06%), Query Frame = 1
Query: 97 KFKAIHIAGTKGKGSTAAFLSSILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKAL 156
K IH+AGTKGKGST F SI+R G+ G +TSPH+ +RER L ++ K L
Sbjct: 111 KMNVIHVAGTKGKGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLD-GVDISEEKFL 170
Query: 157 NFL---FKRNKELLDQSVKLENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDAT 216
+ + R KE ++ + + ++F L +AF +FA E VD A++E GLGG DAT
Sbjct: 171 GYFWWCYNRLKERTNEEIPMP----TYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDAT 230
Query: 217 NIICSSRLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFIPNIECILR 276
N + + I+S+G +HM LG +L IA KAGI K G P +P
Sbjct: 231 NAV--QKPVVCGISSLGYDHMEILGDTLGKIAGEKAGIFKLGVPAFT----VPQ-----P 290
Query: 277 DKALSMSSPVVSASDPGNRSTIKGVSLLNGRLCQCCDLIIQTDNEF------IELFDVNL 336
D+A+ + S ++ ++ V L RL L + ++++ + L + L
Sbjct: 291 DEAMRVLEEKASETE----VNLEVVQPLTARLLSGQKLGLDGEHQYVNAGLAVSLASIWL 350
Query: 337 RMLGRHQLQNAATATCVILTLRNLGWRISDASIRSGLEKTFLVGRSHFLSAKEAGMLGLP 396
+ +G+ ++ + + I GL L GR+ + +
Sbjct: 351 QQIGKLEVPSRTQMS------------ILPEKFIKGLATASLQGRAQVVPDQYTESRTSG 395
Query: 397 GTTILLDGAHTKDSAKA 405
LDGAH+ +S +A
Sbjct: 411 DLVFYLDGAHSPESMEA 395
BLAST of Cp4.1LG14g06540 vs. TAIR10
Match:
AT3G10160.1 (AT3G10160.1 DHFS-FPGS homolog C)
HSP 1 Score: 110.2 bits (274), Expect = 4.2e-24
Identity = 79/208 (37.98%), Postives = 114/208 (54.81%), Query Frame = 1
Query: 97 KFKAIHIAGTKGKGSTAAFLSSILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKAL 156
+ K IH+AGTKGKGST F +ILR G+ G +TSPH+ +RER + ++ K L
Sbjct: 130 ELKVIHVAGTKGKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRID-GLDISEEKFL 189
Query: 157 NFLFKRNKELLDQSVKLENGRISH--FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATN 216
+ ++ K L +++V +G F+ LT +AF +F E VDVAVIE GLGG D+TN
Sbjct: 190 QYFWECWKLLKEKAV---DGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTN 249
Query: 217 IICSSRLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFIPNIECILRD 276
+I + I S+G +HM LG +L IA KAGI K P P + +L+
Sbjct: 250 VI--QKPVVCGIASLGMDHMDILGNTLADIAFHKAGIFKPQIPAFT-VPQLSEAMDVLQK 309
Query: 277 KALSMSSP--VVSASDPGNRSTIKGVSL 301
A ++ P VV+ +P + GV+L
Sbjct: 310 TANNLEVPLEVVAPLEP---KKLDGVTL 327
BLAST of Cp4.1LG14g06540 vs. NCBI nr
Match:
gi|659130858|ref|XP_008465388.1| (PREDICTED: dihydrofolate synthetase isoform X1 [Cucumis melo])
HSP 1 Score: 936.8 bits (2420), Expect = 1.7e-269
Identity = 479/548 (87.41%), Postives = 514/548 (93.80%), Query Frame = 1
Query: 1 MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQQMTEFMEYLDSLK 60
MN+FKYHHH RPQI G LLLN+F+GE PSISSR GSKQCF HSEDQ MTEFMEYLDSLK
Sbjct: 1 MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 60
Query: 61 NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSSIL 120
NYEKLGVP GAGTDS+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGS AAFLS+IL
Sbjct: 61 NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 120
Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
RAEGYSVGCYTSPHIET+RERISLGRSGEMVSGKALN LFKRNKE+ DQSV+LE+GRISH
Sbjct: 121 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 180
Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 240
Query: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSL 300
LESIAMAKAGIIK GCPTILGG F+P IE ILRDKALSMSSPV++ SDPGNRSTIKGV++
Sbjct: 241 LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM 300
Query: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360
LNG LCQCCD++IQ DNEF+EL DVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI
Sbjct: 301 LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360
Query: 361 RSGLEKTFLVGRSHFLSAKEAGMLGLPGTTILLDGAHTKDSAKALVDTIQMSFPDAQLAL 420
RSGLE+TFL+GRSHFL+AKEA +LGLPG TILLDGAHTKDSAKALVDTIQM+FP+A+LAL
Sbjct: 361 RSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLAL 420
Query: 421 VVAMASDKDHNGFATEFLQGGKLESIVLSEANIGGGKSRTTSAALLRDCWIQASNEMGIP 480
V+AMASDK+H FA EFLQGGKLE +VL+EA+IGGGKSRTTSAA LRDCWIQAS E+GIP
Sbjct: 421 VIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIP 480
Query: 481 ISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQRIEGRRGLVVVTGSLHAVS 540
ISLETKDA VS TSKLENRPVLTTETSLL AIKIAAEILKQR +G++ LVVV+GSLHAVS
Sbjct: 481 ISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQSLVVVSGSLHAVS 540
Query: 541 MVLSSLHS 549
MVLSSLHS
Sbjct: 541 MVLSSLHS 548
BLAST of Cp4.1LG14g06540 vs. NCBI nr
Match:
gi|449463470|ref|XP_004149457.1| (PREDICTED: probable dihydrofolate synthetase isoform X2 [Cucumis sativus])
HSP 1 Score: 933.3 bits (2411), Expect = 1.9e-268
Identity = 477/548 (87.04%), Postives = 513/548 (93.61%), Query Frame = 1
Query: 1 MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQQMTEFMEYLDSLK 60
MN+FKYHHH RPQI G LLLN+F+GE PSISSR GSKQCF HSEDQ MT+FMEYLDSLK
Sbjct: 1 MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60
Query: 61 NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSSIL 120
NYEKLGVP G+GTDS+DGFDLGRM+RLMERLGNPQS+FKAIHIAGTKGKGSTAAFLS+IL
Sbjct: 61 NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120
Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
R EGYSVGCYTSPHIETIRERISLGRSG+MVSGKALN LFKRNKE+ DQSV+LENG +SH
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180
Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
FEVLTAMAFSLFAQE+VDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEH+AALGGS
Sbjct: 181 FEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGS 240
Query: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSL 300
LESIA AKAGIIK GCPTILGGPF+P IE ILRDKALSMSSPV+SASDPGNRSTIKGV+L
Sbjct: 241 LESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNL 300
Query: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360
LNG L QCCD++IQ DNEFIEL DVNLRMLG HQLQNAATATCVILTLRNLGWRISDASI
Sbjct: 301 LNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTLRNLGWRISDASI 360
Query: 361 RSGLEKTFLVGRSHFLSAKEAGMLGLPGTTILLDGAHTKDSAKALVDTIQMSFPDAQLAL 420
RSGLE+TFL+GRSHFL+A+EA +LGLPG TILLDGAHTKDSAKAL+DTIQM+FP+AQLAL
Sbjct: 361 RSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLAL 420
Query: 421 VVAMASDKDHNGFATEFLQGGKLESIVLSEANIGGGKSRTTSAALLRDCWIQASNEMGIP 480
VVAMASDK+H GFA EFLQGGKLES+VL+EA IGGGKSRTTSAA LRDCWIQASNE+GIP
Sbjct: 421 VVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRDCWIQASNELGIP 480
Query: 481 ISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQRIEGRRGLVVVTGSLHAVS 540
ISLETKDA V TSKL NRPVLTTETSLL AIKIAAEILKQR +GR+GLVVV+GSLHAVS
Sbjct: 481 ISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQGLVVVSGSLHAVS 540
Query: 541 MVLSSLHS 549
MVL+SLHS
Sbjct: 541 MVLASLHS 548
BLAST of Cp4.1LG14g06540 vs. NCBI nr
Match:
gi|778680896|ref|XP_011651415.1| (PREDICTED: probable dihydrofolate synthetase isoform X1 [Cucumis sativus])
HSP 1 Score: 922.5 bits (2383), Expect = 3.4e-265
Identity = 471/541 (87.06%), Postives = 506/541 (93.53%), Query Frame = 1
Query: 1 MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQQMTEFMEYLDSLK 60
MN+FKYHHH RPQI G LLLN+F+GE PSISSR GSKQCF HSEDQ MT+FMEYLDSLK
Sbjct: 1 MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60
Query: 61 NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSSIL 120
NYEKLGVP G+GTDS+DGFDLGRM+RLMERLGNPQS+FKAIHIAGTKGKGSTAAFLS+IL
Sbjct: 61 NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120
Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
R EGYSVGCYTSPHIETIRERISLGRSG+MVSGKALN LFKRNKE+ DQSV+LENG +SH
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180
Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
FEVLTAMAFSLFAQE+VDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEH+AALGGS
Sbjct: 181 FEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGS 240
Query: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSL 300
LESIA AKAGIIK GCPTILGGPF+P IE ILRDKALSMSSPV+SASDPGNRSTIKGV+L
Sbjct: 241 LESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNL 300
Query: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360
LNG L QCCD++IQ DNEFIEL DVNLRMLG HQLQNAATATCVILTLRNLGWRISDASI
Sbjct: 301 LNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTLRNLGWRISDASI 360
Query: 361 RSGLEKTFLVGRSHFLSAKEAGMLGLPGTTILLDGAHTKDSAKALVDTIQMSFPDAQLAL 420
RSGLE+TFL+GRSHFL+A+EA +LGLPG TILLDGAHTKDSAKAL+DTIQM+FP+AQLAL
Sbjct: 361 RSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLAL 420
Query: 421 VVAMASDKDHNGFATEFLQGGKLESIVLSEANIGGGKSRTTSAALLRDCWIQASNEMGIP 480
VVAMASDK+H GFA EFLQGGKLES+VL+EA IGGGKSRTTSAA LRDCWIQASNE+GIP
Sbjct: 421 VVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRDCWIQASNELGIP 480
Query: 481 ISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQRIEGRRGLVVVTGSLHAVS 540
ISLETKDA V TSKL NRPVLTTETSLL AIKIAAEILKQR +GR+GLVVV+GSLHAVS
Sbjct: 481 ISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQGLVVVSGSLHAVS 540
Query: 541 M 542
M
Sbjct: 541 M 541
BLAST of Cp4.1LG14g06540 vs. NCBI nr
Match:
gi|659130860|ref|XP_008465389.1| (PREDICTED: dihydrofolate synthetase isoform X2 [Cucumis melo])
HSP 1 Score: 698.0 bits (1800), Expect = 1.3e-197
Identity = 350/395 (88.61%), Postives = 372/395 (94.18%), Query Frame = 1
Query: 1 MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQQMTEFMEYLDSLK 60
MN+FKYHHH RPQI G LLLN+F+GE PSISSR GSKQCF HSEDQ MTEFMEYLDSLK
Sbjct: 1 MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 60
Query: 61 NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSSIL 120
NYEKLGVP GAGTDS+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGS AAFLS+IL
Sbjct: 61 NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 120
Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
RAEGYSVGCYTSPHIET+RERISLGRSGEMVSGKALN LFKRNKE+ DQSV+LE+GRISH
Sbjct: 121 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 180
Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 240
Query: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSL 300
LESIAMAKAGIIK GCPTILGG F+P IE ILRDKALSMSSPV++ SDPGNRSTIKGV++
Sbjct: 241 LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM 300
Query: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360
LNG LCQCCD++IQ DNEF+EL DVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI
Sbjct: 301 LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360
Query: 361 RSGLEKTFLVGRSHFLSAKEAGMLGLPGTTILLDG 396
RSGLE+TFL+GRSHFL+AKEA +LGLPG TILLDG
Sbjct: 361 RSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDG 395
BLAST of Cp4.1LG14g06540 vs. NCBI nr
Match:
gi|1009122795|ref|XP_015878195.1| (PREDICTED: dihydrofolate synthetase [Ziziphus jujuba])
HSP 1 Score: 694.1 bits (1790), Expect = 1.9e-196
Identity = 357/523 (68.26%), Postives = 429/523 (82.03%), Query Frame = 1
Query: 36 SKQCFGNHSEDQQMTEFMEYLDSLKNYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQ 95
S++ SED + +FMEYLD+LKNYEK GVP AGTDS++GFDLGRM+RLME LGNPQ
Sbjct: 35 SRKSLCTRSEDTVLKDFMEYLDALKNYEKSGVPKSAGTDSDEGFDLGRMRRLMELLGNPQ 94
Query: 96 SKFKAIHIAGTKGKGSTAAFLSSILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKA 155
S FKA+HIAG+KGKGSTAAFLSSILRAEGYSVGCYTSPHI+TIRERISLGR GE V+ KA
Sbjct: 95 SNFKAVHIAGSKGKGSTAAFLSSILRAEGYSVGCYTSPHIQTIRERISLGRFGEPVAAKA 154
Query: 156 LNFLFKRNKELLDQSVKLENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNI 215
LN LF R K+++DQ+V+LENG ISHFEVLTAMAF+LFAQENVD+AVIEAGLGGARDATN+
Sbjct: 155 LNSLFNRTKKIIDQAVELENGCISHFEVLTAMAFTLFAQENVDIAVIEAGLGGARDATNV 214
Query: 216 ICSSRLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFIPNIECILRDK 275
I SS LA +VIT+IGEEHMAALGGSLESIA+AK+GIIKHGCP +LGGPF+P+IECILR+K
Sbjct: 215 ISSSGLALSVITTIGEEHMAALGGSLESIAVAKSGIIKHGCPVVLGGPFLPHIECILRNK 274
Query: 276 ALSMSSPVVSASDPGNRSTIKGVSLLNGRLCQCCDLIIQTDNE---FIELFDVNLRMLGR 335
A SM SPV+SA D GN+S IKG+S+ NGR CQ CD++IQ ++E FIELFDV L MLG
Sbjct: 275 ASSMHSPVISAYDTGNQSKIKGISIHNGRPCQSCDIVIQVESEINLFIELFDVQLYMLGS 334
Query: 336 HQLQNAATATCVILTLRNLGWRISDASIRSGLEKTFLVGRSHFLSAKEAGMLGLPGTTIL 395
HQLQNAATATC L LRNLGW+ISD SI+ GL+ T L+GRS FL++KEA LG+ I+
Sbjct: 335 HQLQNAATATCAALCLRNLGWKISDRSIKDGLQHTHLLGRSQFLTSKEAEALGVSKPMIM 394
Query: 396 LDGAHTKDSAKALVDTIQMSFPDAQLALVVAMASDKDHNGFATEFLQGGKLESIVLSEAN 455
LDGAHTK+SAKALV+TIQM+FP A+LALVVAMASDKDH GFA EFL GG+LE ++L+EA+
Sbjct: 395 LDGAHTKESAKALVETIQMTFPRARLALVVAMASDKDHVGFAREFLSGGQLEGVLLTEAD 454
Query: 456 IGGGKSRTTSAALLRDCWIQASNEMGIPISLET--------KDAPVSSTSKLENRPVLTT 515
I GGKSRT +A+ LRDCWI+AS E+GI + + D S S ++NR +L
Sbjct: 455 IAGGKSRTAAASFLRDCWIKASEELGITLVHDKMSDYQELFNDQLDCSASTIQNRTILAV 514
Query: 516 ETSLLRAIKIAAEILKQRIEGRRGLVVVTGSLHAVSMVLSSLH 548
E S L ++KIA +IL++R G++VVTGSLH VS+VL++LH
Sbjct: 515 EASFLSSMKIADQILRKRTGNGFGIIVVTGSLHIVSLVLATLH 557
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| DHFS_ARATH | 6.7e-157 | 59.92 | Dihydrofolate synthetase OS=Arabidopsis thaliana GN=DHFS PE=1 SV=1 | [more] |
| FOLC_BACSU | 3.1e-45 | 35.31 | Folylpolyglutamate synthase OS=Bacillus subtilis (strain 168) GN=folC PE=3 SV=2 | [more] |
| FOLCP_HALVD | 1.5e-31 | 29.87 | Probable bifunctional folylpolyglutamate synthase/dihydropteroate synthase OS=Ha... | [more] |
| FOLC_LACCA | 3.4e-31 | 27.58 | Folylpolyglutamate synthase OS=Lactobacillus casei GN=fgs PE=1 SV=1 | [more] |
| FOLD_SCHPO | 1.3e-30 | 32.97 | Probable dihydrofolate synthetase OS=Schizosaccharomyces pombe (strain 972 / ATC... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0L7A4_CUCSA | 1.3e-268 | 87.04 | Uncharacterized protein OS=Cucumis sativus GN=Csa_3G354540 PE=4 SV=1 | [more] |
| A0A061G8S9_THECC | 6.1e-189 | 65.21 | Folylpolyglutamate synthetase family protein isoform 2 OS=Theobroma cacao GN=TCM... | [more] |
| E0CUK0_VITVI | 5.1e-188 | 65.84 | Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0050g02490 PE=4 SV=... | [more] |
| A0A0D2Q2P9_GOSRA | 1.3e-183 | 62.71 | Uncharacterized protein OS=Gossypium raimondii GN=B456_008G270800 PE=4 SV=1 | [more] |
| U5FM82_POPTR | 2.2e-183 | 66.28 | Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0015s06150g PE=4 SV=1 | [more] |
