Cucsa.010800 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.010800
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Trans-2,3-enoyl-CoA reductase
Location	scaffold00154 : 189575 .. 193311 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CACACTTTCTCTTCACTAATCCATGGCAATTTTTTtCTTCCATTAAGAATTTGGGTTCACGTTCATCCCAATAAATTTATTCAGTTGGCTGTCGCCTCCAGCATCGCAGATCAAACGATGTGTCCAATTGCAATTAGGAGATGATTAGCGACACGGTTCATCAGTCATCCGAATTAGCTCTCCTACCCCAACTGCACACCATTTAATAGCCTACTTCCCAACCTCCCACTTCAAACCCAGAAACCTTTTCCTATTTTAAATTCACACCCATTTGCGGATCTCATCATATATCCATTTTTTGGGTTCTGGGTCTTCTCATTTTGCTCTTTTCAGGATCTGGGTACAAATTTTTGGCTTTTTTCTCCAACTGGTTCTCTAATTTGGCTGCAGAAATGAAGGTCTCGGTAGTTTCTCGCAGTGGGAGAGAGGTTGTTAAAGGGGGTATTCAACTGAGCGACTCTGTAAGTGTTTTGGATTTTGATTGAATTACTGTTTTTGTTTACTTCTCTTCTTGATTCTTGGAAAGAGGAAAGAATTAGAAGATTACTACTTGTTGTTGGTGCCTGTGTTCTGTTTTTAGACCTATTAATTTTCCTTCTTACAGATGAGTTGAAGGTGGGACTTTGACTTTTATGGAAATTGTTGTtTtGTTTTTtGTAGGCTTCGGTGGCAGATCTGCAGGATGCGATTCATAAGCGAAGTAAGTGCAAGATAGTAGAATGGTATTCCTTTAATTGTTGCATACATGCACTTTTATTAGTTTAGATTTGTATTTTTATGGTAATGATTGACAGAACTGGAGTTTGTGAATAGGGGTGGAAAAATTCGAACCAGCATATGCATTAACTAGTTGAGCTCTGCTGTCGTTGCTTATGTAATGTATCTACTTGTGGTTTGCCCCCATTTTTTTTTtGTAGTTATTAAAATGATGAATTTAAAAGATTAAGTTCGGTCTTCATTGTAGCTTATCAGTTTAAGCTTTGGGGTATGGAGTTGCTGAGTAGTCAATATTTTGTTATCAGTACATAAATGGTAAAGTATTTGAAGAAGTCCATTGTTTTTTTTtCCTATGCAATGCTTTCAGAAAATTGGTCTTTTATTACTTCTAGGATAACAACAGACGAGAATATGCCTATTGGGCTCGGGTATGCTGATCTTTTCTGCCTTTTTCTAACTATATGTCCATCGTTCTTGATTATATTGCGAAGAGTTATCATGTCTATAGACTTAGAATTAGCAAAAGATAAAATGATTTCGTTATGGGTGCTTAGTTGCTTTTTCTTTTCCTAGGATGGGATGTAAAATACCTTTTGGGGTTCAATATATAATTGCTAATGCACCTTAATGGCTTTAAATCATTATTCATATCAGTGTGGTTGTGATGTATAAACTAATGAAAAGTATCTTTCCTTTCAATTTTCTTGCAAATTGCATACCCAGCATTCTAGATACTACATAGGCAATCCCCTTCCCTAGCAAGGTGATAGCCCCCAATTGGGAAGGTGTACATGTAGGAGAAAAGGAAAGGATTGAGTGAATGAGAAAAGTTACATGGACGTAGTTGGCTCCAAGGTATTTTGTTTGCTAGAATGGGACGAAGGGATTGTTATACAGGTGGTTTTTCTTTCTTGGCAGATGGGGGAGAATTTTTGTGTATACTCTCCAGAGAATTGGGAGAATTAAGCTTCCTAGTACCTTGCTATCTGATCATAAATAAAATTGCTGTGCAAGCCCTATCACAGGATTTTTTCCATCATAAATTTGAAAGGAGATGTCGTTTTCATGTTTAAAATTTCTATATAAATCCCAATGGTCATCTTAGTACCATGTTGGTGTAATTTGTTTCCTTCTTTTTTtAAAATAATTAATGCTGCAGTTGCATCATGCCATTAGGTTCACTTTTGATATGCATTGTTGCTAGGGTGGGGTGGAGAATTTTCTTTTTGCATTGCCTACTTGATATTGACTTTGAAGCTCAGCTTCATTGCGACATGCCTCTTTTGTTGTCAGCTAAGATTAGACATAAAGTCCCTTAACGACTTTTACTGTCAATAGAATCCTCCATTCCAGTGTGAGTTTTATGAAGGAAAAaCAAAAaGAAATTTAAAAGGACTTATGCTTGATAGTCTCTGTGGTTCTTATAACAGGAACATTTGGTATTTTTGCCTGATATTGTTGGATGTTTCTTGATCAGTGGCAAGCTGCCTGTCATTTAGGATGCATACATGCATTCCAATGTCAGTTTTTATTTAAAGTACAGCTATCTGATTTCCTTGTTTTGTTGAAACAGCCAAAAAGTTCTATCCAGCTAGGCAAAGGCTAACTCTTCCCGTTCAACCTGGTTCAAAGGAGAGACCTACTGTTCTTAACTCCAAGAAAAGCCTCAGAGAATATTGTAGCGACAACTCGGACAGCATAACCGTTGTCTTCAAGGATTTGGGCCCACAAGTTTCTTATCGTACACTCTTTTTCTTTGAATACTTGGGTCCACTGATTCTCTACCCCATCTTTTACTACTTCCCTGTATATCAGTTCTTTGGCTACAAAGCAGAACGTGTTATCCATCCTGTTCAGACTTACGCAATGTACTACTGGTGTTTTCATTATTTCAAAAGAATCATGGAAACGTTTTTCGTCCATAGGTTCAGCCACGCAACCTCACCACTTTCGAATGTGTTCAGAAATTGTGCTTACTACTGGTCTTTTGGCTCGTACATTGCTTATTATGTCAATCACCCACTTTATACCCCTGTTGGCGATCTTCAGATGAAGATTGGCTTTGCATTTGGTTTGCTGTGTCAAGTGTCAAACTTCTACTGCCATATCTTGCTGAGAAAATTGCGTAGCCCTGAAGGCAATGGTGGATATCAAATCCCTAAGGGGTTTCTGTTCAACATTGTGACATGTGCGAATTACACAACAGAAATATATCAGTGGGTAGGCTTCAATATAGCTACACAGACTTTTGCTGGTTATGTCTTTCTTGTGGTTGCTGCTCTTATCATGACTAACTGGGCACTTGCAAAGCACCGTCGCCTCAAGAAGGTACCATCTTACTATCTTTTCTAGCAGTTTAAGCTTTTAGGTCTTTTGGTAATTAGGTACTATGTTAAATCACCTCCAAATCGCAAAGCTTTAAGTTGATAAATTTACACCCCCTCCCTCGTCAGTGGACTTAGGAACTAACACAAGATCTAATAAGTGTATAATCACTATTAATTAGGTTGAAGATAGTAGCTCTAGCTTTGCTACTATACTAAATCACTACTAAATTCAAAGGCTTGAACCGATGATGTCATTTGCTATGTTCTGGGTTTAAACGTGCGGTACTCTTGGACTAAATAAACCTCTCTATGAGTTATGAAATCCAGAGATTCCTCTCACATGTTTGGATCTTTAGTAGCATCTTGTAGTGATGTTGGTTATGATTTGAACATGAATTGATTTGTATTGTCTATGCAGCTGTTTGATGGGAAAGAGGGAAGGCCAAAGTATCCACGCAGATGGGTGATTCTTCCTCCATTTCTGTAGAAGCATTTGGTGGCCTCAGCAACCAATTCAATCTCAAGATGGTAGGCTTTAGATTAAAAGTGTCACTTGAAAGAAAGACAGAGACAAGGCACAGACATCACTTTTACCAAATGCAATTTTAATGTTCTTTTGTTGTTGTCTTTAACATTATAGACAATGTTCCTTTtGTTTTGCTTTGGCGTTTTAGTGCATTGTTGGTGATTGAT

mRNA sequence

CACACTTTCTCTTCACTAATCCATGGCAATTTTTTTCTTCCATTAAGAATTTGGGTTCACGTTCATCCCAATAAATTTATTCAGTTGGCTGTCGCCTCCAGCATCGCAGATCAAACGATGTGTCCAATTGCAATTAGGAGATGATTAGCGACACGGTTCATCAGTCATCCGAATTAGCTCTCCTACCCCAACTGCACACCATTTAATAGCCTACTTCCCAACCTCCCACTTCAAACCCAGAAACCTTTTCCTATTTTAAATTCACACCCATTTGCGGATCTCATCATATATCCATTTTTTGGGTTCTGGGTCTTCTCATTTTGCTCTTTTCAGGATCTGGGTACAAATTTTTGGCTTTTTTCTCCAACTGGTTCTCTAATTTGGCTGCAGAAATGAAGGTCTCGGTAGTTTCTCGCAGTGGGAGAGAGGTTGTTAAAGGGGGTATTCAACTGAGCGACTCTGCTTCGGTGGCAGATCTGCAGGATGCGATTCATAAGCGAACCAAAAAGTTCTATCCAGCTAGGCAAAGGCTAACTCTTCCCGTTCAACCTGGTTCAAAGGAGAGACCTACTGTTCTTAACTCCAAGAAAAGCCTCAGAGAATATTGTAGCGACAACTCGGACAGCATAACCGTTGTCTTCAAGGATTTGGGCCCACAAGTTTCTTATCGTACACTCTTTTTCTTTGAATACTTGGGTCCACTGATTCTCTACCCCATCTTTTACTACTTCCCTGTATATCAGTTCTTTGGCTACAAAGCAGAACGTGTTATCCATCCTGTTCAGACTTACGCAATGTACTACTGGTGTTTTCATTATTTCAAAAGAATCATGGAAACGTTTTTCGTCCATAGGTTCAGCCACGCAACCTCACCACTTTCGAATGTGTTCAGAAATTGTGCTTACTACTGGTCTTTTGGCTCGTACATTGCTTATTATGTCAATCACCCACTTTATACCCCTGTTGGCGATCTTCAGATGAAGATTGGCTTTGCATTTGGTTTGCTGTGTCAAGTGTCAAACTTCTACTGCCATATCTTGCTGAGAAAATTGCGTAGCCCTGAAGGCAATGGTGGATATCAAATCCCTAAGGGGTTTCTGTTCAACATTGTGACATGTGCGAATTACACAACAGAAATATATCAGTGGGTAGGCTTCAATATAGCTACACAGACTTTTGCTGGTTATGTCTTTCTTGTGGTTGCTGCTCTTATCATGACTAACTGGGCACTTGCAAAGCACCGTCGCCTCAAGAAGCTGTTTGATGGGAAAGAGGGAAGGCCAAAGTATCCACGCAGATGGGTGATTCTTCCTCCATTTCTGTAGAAGCATTTGGTGGCCTCAGCAACCAATTCAATCTCAAGATGGTAGGCTTTAGATTAAAAGTGTCACTTGAAAGAAAGACAGAGACAAGGCACAGACATCACTTTTACCAAATGCAATTTTAATGTTCTTTTGTTGTTGTCTTTAACATTATAGACAATGTTCCTTTTGTTTTGCTTTGGCGTTTTAGTGCATTGTTGGTGATTGAT

Coding sequence (CDS)

ATGAAGGTCTCGGTAGTTTCTCGCAGTGGGAGAGAGGTTGTTAAAGGGGGTATTCAACTGAGCGACTCTGCTTCGGTGGCAGATCTGCAGGATGCGATTCATAAGCGAACCAAAAAGTTCTATCCAGCTAGGCAAAGGCTAACTCTTCCCGTTCAACCTGGTTCAAAGGAGAGACCTACTGTTCTTAACTCCAAGAAAAGCCTCAGAGAATATTGTAGCGACAACTCGGACAGCATAACCGTTGTCTTCAAGGATTTGGGCCCACAAGTTTCTTATCGTACACTCTTTTTCTTTGAATACTTGGGTCCACTGATTCTCTACCCCATCTTTTACTACTTCCCTGTATATCAGTTCTTTGGCTACAAAGCAGAACGTGTTATCCATCCTGTTCAGACTTACGCAATGTACTACTGGTGTTTTCATTATTTCAAAAGAATCATGGAAACGTTTTTCGTCCATAGGTTCAGCCACGCAACCTCACCACTTTCGAATGTGTTCAGAAATTGTGCTTACTACTGGTCTTTTGGCTCGTACATTGCTTATTATGTCAATCACCCACTTTATACCCCTGTTGGCGATCTTCAGATGAAGATTGGCTTTGCATTTGGTTTGCTGTGTCAAGTGTCAAACTTCTACTGCCATATCTTGCTGAGAAAATTGCGTAGCCCTGAAGGCAATGGTGGATATCAAATCCCTAAGGGGTTTCTGTTCAACATTGTGACATGTGCGAATTACACAACAGAAATATATCAGTGGGTAGGCTTCAATATAGCTACACAGACTTTTGCTGGTTATGTCTTTCTTGTGGTTGCTGCTCTTATCATGACTAACTGGGCACTTGCAAAGCACCGTCGCCTCAAGAAGCTGTTTGATGGGAAAGAGGGAAGGCCAAAGTATCCACGCAGATGGGTGATTCTTCCTCCATTTCTGTAG

Protein sequence

MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPTVLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYPRRWVILPPFL*

Homology

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BLAST of Cucsa.010800 vs. Swiss-Prot
Match: TECR_ARATH (Very-long-chain enoyl-CoA reductase OS=Arabidopsis thaliana GN=ECR PE=1 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 7.0e-159
Identity = 255/310 (82.26%), Postives = 284/310 (91.61%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60
           MKV+VVSRSGREV+K  + L DSA+VADLQ+A HKR KKFYP+RQRLTLPV PGSK++P 
Sbjct: 1   MKVTVVSRSGREVLKAPLDLPDSATVADLQEAFHKRAKKFYPSRQRLTLPVTPGSKDKPV 60

Query: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
           VLNSKKSL+EYC  N++S+TVVFKDLG QVSYRTLFFFEYLGPL++YP+FYYFPVY+F G
Sbjct: 61  VLNSKKSLKEYCDGNNNSLTVVFKDLGAQVSYRTLFFFEYLGPLLIYPVFYYFPVYKFLG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180
           Y  + VIHPVQTYAMYYWCFHYFKRI+ETFFVHRFSHATSP+ NVFRNCAYYWSFG+YIA
Sbjct: 121 YGEDCVIHPVQTYAMYYWCFHYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYIA 180

Query: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLYTPV DLQMKIGF FGL+CQV+NFYCHILL+ LR P G GGYQIP+GFLFNIV
Sbjct: 181 YYVNHPLYTPVSDLQMKIGFGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300
           TCANYTTEIYQW+GFNIATQT AGYVFL VAALIMTNWAL KH RL+K+FDGK+G+PKYP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTIAGYVFLAVAALIMTNWALGKHSRLRKIFDGKDGKPKYP 300

Query: 301 RRWVILPPFL 311
           RRWVILPPFL
Sbjct: 301 RRWVILPPFL 310

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BLAST of Cucsa.010800 vs. Swiss-Prot
Match: TECR_DICDI (Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum GN=gpsn2 PE=3 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 2.9e-56
Identity = 122/310 (39.35%), Postives = 178/310 (57.42%), Query Frame = 1


Query: 3   VSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPTVL 62
           + VVS+  ++ V G    S S +V +L+  I  +T+      +R+ L V   + + P   
Sbjct: 4   IKVVSQRSKKEV-GSFSTSSSTTVGELKKQISSKTRL---GTERIRLAVPSKTSKLPNAF 63

Query: 63  NSKKSLREYCSDN--SDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 122
            +     +  S +  +DS T+ FKDLGPQ+S+  +F  EY GPL +YPIFY+      +G
Sbjct: 64  EALGKDSDLVSKHVGADS-TLYFKDLGPQISWSLVFICEYAGPLFVYPIFYFLSNL-IYG 123

Query: 123 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 182
             + +     Q  A+  +  HY KRI ET FVHRFSH T P+ N+F+NC+YYW   + ++
Sbjct: 124 TDSPKSF--AQKVALVCYSLHYIKRIYETIFVHRFSHGTMPIFNLFKNCSYYWGCTAMVS 183

Query: 183 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 242
           Y+VNHPLYT     ++ +G    ++ +V N+ CHI LR LR P G+   +IP+G LF  V
Sbjct: 184 YFVNHPLYTEAPIERVYLGLGLWIIGEVFNYICHIQLRNLR-PAGSTERKIPRGLLFEFV 243

Query: 243 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 302
           +C NYT EI  W+GF+I TQT   ++F ++ A  M  WA+ KHRR +K F       KYP
Sbjct: 244 SCPNYTVEILSWIGFSILTQTLTSWIFALMGAAQMWIWAVGKHRRYRKEFG-----DKYP 299

Query: 303 RRWVILPPFL 311
           +   IL PFL
Sbjct: 304 KSRKILIPFL 299

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BLAST of Cucsa.010800 vs. Swiss-Prot
Match: TECR_HUMAN (Very-long-chain enoyl-CoA reductase OS=Homo sapiens GN=TECR PE=1 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 6.3e-51
Identity = 122/316 (38.61%), Postives = 173/316 (54.75%), Query Frame = 1


Query: 2   KVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPTV 61
           +V ++    RE +    ++   A++A++++   K   ++YPARQ L L  +  S +   V
Sbjct: 5   EVEILDAKTREKLCFLDKVEPHATIAEIKNLFTKTHPQWYPARQSLRLDPKGKSLKDEDV 64

Query: 62  LNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFGY 121
           L               + T+ F+DLG Q+S+ T+F  EY GPL +Y +FY F V   +G+
Sbjct: 65  LQKLPV--------GTTATLYFRDLGAQISWVTVFLTEYAGPLFIYLLFY-FRVPFIYGH 124

Query: 122 KAERVI--HPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYI 181
           K +     H V   A     FHY KR++ET FVHRFSH T PL N+F+NC YYW F +++
Sbjct: 125 KYDFTSSRHTVVHLACICHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWM 184

Query: 182 AYYVNHPLYTP--VGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPK---- 241
           AYY+NHPLYTP   G  Q+K+  A  ++CQ+ NF  H+ LR LR P G+   +IP     
Sbjct: 185 AYYINHPLYTPPTYGAQQVKLALAIFVICQLGNFSIHMALRDLR-PAGSKTRKIPYPTKN 244

Query: 242 --GFLFNIVTCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFD 301
              +LF +V+C NYT E+  W+GF I TQ     +F +V    MT WA  KHR   K F 
Sbjct: 245 PFTWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFSLVGFTQMTIWAKGKHRSYLKEF- 304

Query: 302 GKEGRPKYPRRWVILP 308
               R   P R  I+P
Sbjct: 305 ----RDYPPLRMPIIP 305

[Close]

BLAST of Cucsa.010800 vs. Swiss-Prot
Match: TECR_BOVIN (Very-long-chain enoyl-CoA reductase OS=Bos taurus GN=TECR PE=2 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.4e-50
Identity = 122/316 (38.61%), Postives = 172/316 (54.43%), Query Frame = 1


Query: 2   KVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPTV 61
           +V ++    RE +    ++   A++A++++   K   ++YPARQ L L  +  S +   V
Sbjct: 5   EVEILDAKTREKLCFLDKVEPQATIAEIKNLFTKTHPQWYPARQSLRLDPKGKSLKDEDV 64

Query: 62  LNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFGY 121
           L               + T+ F+DLG Q+S+ T+F  EY GPL +Y +FY F V   +G 
Sbjct: 65  LQKLPV--------GTTATLYFRDLGAQISWVTVFLTEYAGPLFIYLLFY-FRVPFIYGR 124

Query: 122 KAERVI--HPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYI 181
           K +     H V   A     FHY KR++ET FVHRFSH T PL N+F+NC YYW F +++
Sbjct: 125 KYDFTSSRHTVVHLACICHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWM 184

Query: 182 AYYVNHPLYTP--VGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPK---- 241
           AYY+NHPLYTP   G  Q+K+  A  ++CQ+ NF  H+ LR LR P G+   +IP     
Sbjct: 185 AYYINHPLYTPPTYGAQQVKLALAIFVICQLGNFSIHMALRDLR-PAGSKTRKIPYPTRN 244

Query: 242 --GFLFNIVTCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFD 301
              +LF +V+C NYT E+  W+GF I TQ     +F +V    MT WA  KHR   K F 
Sbjct: 245 PFTWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFSLVGFTQMTIWAKGKHRSYLKEF- 304

Query: 302 GKEGRPKYPRRWVILP 308
               R   P R  I+P
Sbjct: 305 ----RDYPPLRMPIIP 305

[Close]

BLAST of Cucsa.010800 vs. Swiss-Prot
Match: TECR_CAEEL (Probable very-long-chain enoyl-CoA reductase art-1 OS=Caenorhabditis elegans GN=art-1 PE=3 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 3.1e-50
Identity = 111/289 (38.41%), Postives = 155/289 (53.63%), Query Frame = 1


Query: 32  AIHKRT--KKFYPARQRLTLPVQPGSKERPTVLNSKKSLREYCSDNSDSITVVFKDLGPQ 91
           AI KR   KK     +R  L V+P  K +P   + K S       +S    +  +DLGPQ
Sbjct: 31  AIKKRIAQKKLKLTEERQALRVEP--KGKPLADDQKLS---DLGLSSQKAVLYVRDLGPQ 90

Query: 92  VSYRTLFFFEYLGPLILYPIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIMET 151
           ++++T+F  EY GPL +YP+FY  P + +        +HP    A + W FHY KR+ ET
Sbjct: 91  IAWKTVFMAEYAGPLFVYPLFYLRPTFIYGQAAVNATMHPAVQIAFFAWSFHYAKRLFET 150

Query: 152 FFVHRFSHATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTP--VGDLQMKIGFAFGLLCQ 211
            F+HRF ++T P  N+ +NC+YYW F +++AY+VNHPL+TP   GDLQ+  G A  ++ +
Sbjct: 151 QFIHRFGNSTMPQFNLVKNCSYYWGFAAFVAYFVNHPLFTPPAFGDLQVYFGLAGFVISE 210

Query: 212 VSNFYCHILLRKLRSPEGNGGYQIPK------GFLFNIVTCANYTTEIYQWVGFNIATQT 271
             N   HILLR LR P G    +IPK        LFN V+C NYT E+  W+ F+I  Q+
Sbjct: 211 FGNLSIHILLRNLR-PAGTRERRIPKPDGNPLSLLFNYVSCPNYTYEVASWIFFSIMVQS 270

Query: 272 FAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYPRRWVILPPFL 311
               +F       M  WA  KHR   K F      P YP+    + PF+
Sbjct: 271 LPAIIFTTAGFAQMAIWAQGKHRNYLKEF------PDYPKNRKAIVPFV 307

[Close]

BLAST of Cucsa.010800 vs. TrEMBL
Match: A0A0A0KNT8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G517090 PE=4 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 2.8e-183
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60
           MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60

Query: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
           VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG
Sbjct: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180
           YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA
Sbjct: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180

Query: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV
Sbjct: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300
           TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP
Sbjct: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300

Query: 301 RRWVILPPFL 311
           RRWVILPPFL
Sbjct: 301 RRWVILPPFL 310

[Close]

BLAST of Cucsa.010800 vs. TrEMBL
Match: B9SXZ3_RICCO (Synaptic glycoprotein SC2, putative OS=Ricinus communis GN=RCOM_1206840 PE=4 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 2.2e-167
Identity = 272/310 (87.74%), Postives = 299/310 (96.45%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60
           MKV+VVSRSGREV+KGG+ L+DSA+VADLQ+AIHKRTKKFYP+RQRLTLP+ PGSK+RPT
Sbjct: 1   MKVTVVSRSGREVIKGGLDLNDSATVADLQEAIHKRTKKFYPSRQRLTLPLPPGSKDRPT 60

Query: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
           VLN KKSL++YC  NSD+ITVVFKDLGPQVSYRTLFFFEYLGPLILYP+FY+FPVY++FG
Sbjct: 61  VLNYKKSLKDYCDGNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPVFYFFPVYKYFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180
           YK ERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYW+FGSYIA
Sbjct: 121 YKEERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIA 180

Query: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLYTPVGDLQMKIGF FGL+CQ++NFYCHILL+ LRSP+G+GGYQIP GFLFNIV
Sbjct: 181 YYVNHPLYTPVGDLQMKIGFGFGLICQIANFYCHILLKNLRSPDGSGGYQIPHGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300
           TCANYTTEIYQW+GFNIATQT AGY+FLVVAA IMTNWALAKHRRL+KLFDGK+GRPKYP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTVAGYIFLVVAASIMTNWALAKHRRLRKLFDGKDGRPKYP 300

Query: 301 RRWVILPPFL 311
           RRWVILPPFL
Sbjct: 301 RRWVILPPFL 310

[Close]

BLAST of Cucsa.010800 vs. TrEMBL
Match: A9PDL9_POPTR (3-oxo-5-alpha-steroid 4-dehydrogenase family protein OS=Populus trichocarpa GN=POPTR_0008s05550g PE=2 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 1.0e-164
Identity = 269/310 (86.77%), Postives = 293/310 (94.52%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60
           MKV+VVSRSGREV+KGG++LSDSA+VADLQ+AIHKRTKKFYPARQRLTLP+ PGSKERPT
Sbjct: 1   MKVTVVSRSGREVIKGGLELSDSATVADLQEAIHKRTKKFYPARQRLTLPLPPGSKERPT 60

Query: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
           VLN KK+L+EY   N DS+T++FKDLGPQVSYRTLFFFEYLGPL+LYP FYYFPVYQF G
Sbjct: 61  VLNYKKNLKEYQDGNLDSLTILFKDLGPQVSYRTLFFFEYLGPLVLYPFFYYFPVYQFLG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180
           YK E ++HPVQTYA+YYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYW+FGSYIA
Sbjct: 121 YKGEHIMHPVQTYALYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIA 180

Query: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLYTPVGDLQMKIGF FGL+CQ++NFYCHILLR LR P+GNGGYQIP GFLFNIV
Sbjct: 181 YYVNHPLYTPVGDLQMKIGFGFGLVCQLANFYCHILLRNLRRPDGNGGYQIPSGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300
           TCANYTTEIYQW+GFNIATQT AGY+FLVVAA IMTNWALAKHRRLKKLFDGK+GRPKYP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTVAGYIFLVVAASIMTNWALAKHRRLKKLFDGKDGRPKYP 300

Query: 301 RRWVILPPFL 311
           RRWVILPP L
Sbjct: 301 RRWVILPPVL 310

[Close]

BLAST of Cucsa.010800 vs. TrEMBL
Match: M9T3Q3_MALDO (Enoyl-ACP reductase OS=Malus domestica PE=2 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 1.7e-164
Identity = 268/310 (86.45%), Postives = 296/310 (95.48%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60
           MKV+VVSRSGREVVKGG++L+DSA+VADLQDAI+KRTKKFYPARQRLTLPVQPGSKERP 
Sbjct: 1   MKVTVVSRSGREVVKGGLELTDSATVADLQDAIYKRTKKFYPARQRLTLPVQPGSKERPV 60

Query: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
           VL+ KKSL++Y S NSD++TVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVY + G
Sbjct: 61  VLSYKKSLQDYISGNSDNLTVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYDYLG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180
           +K +RVIHPVQTYA+YYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFG++IA
Sbjct: 121 FKGDRVIHPVQTYALYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGAFIA 180

Query: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YY+NHPLYTPV DLQMKIGF FG++CQ+SN YCHILLR LRSP+GNGGYQIP+GFLFNIV
Sbjct: 181 YYLNHPLYTPVSDLQMKIGFGFGIICQISNLYCHILLRNLRSPDGNGGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300
           TCANYTTEIYQW+GFNIATQT AGY+FL+VAA IMTNWAL KHRRLKK+FDGK+GRPKYP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTVAGYIFLIVAASIMTNWALTKHRRLKKIFDGKDGRPKYP 300

Query: 301 RRWVILPPFL 311
           RRWVILPPFL
Sbjct: 301 RRWVILPPFL 310

[Close]

BLAST of Cucsa.010800 vs. TrEMBL
Match: A0A059BI33_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_G02931 PE=4 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 2.3e-164
Identity = 271/310 (87.42%), Postives = 293/310 (94.52%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60
           MKVSVVSRSGREVVKGG++L+DSA+VADLQ  IH+RTKKFYP+RQRLTLP+QPGSKE+P 
Sbjct: 1   MKVSVVSRSGREVVKGGVELNDSATVADLQKEIHRRTKKFYPSRQRLTLPLQPGSKEKPI 60

Query: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
            LN KKSL  Y   NS ++TVVFKDLGPQVSYRTLFF+EYLGPLILYPIFY+FPVY++FG
Sbjct: 61  ALNYKKSLESYTEGNSANLTVVFKDLGPQVSYRTLFFWEYLGPLILYPIFYFFPVYRYFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180
           Y  ER+IHPVQTYA+YYWCFHYFKRIMETFFVHRFSHATSP+SNVFRNCAYYWSFGSYIA
Sbjct: 121 YAGERIIHPVQTYALYYWCFHYFKRIMETFFVHRFSHATSPVSNVFRNCAYYWSFGSYIA 180

Query: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLYTPV DLQMKIGFAFG+LCQVSNFYCHILLR LRSP+GNGGYQIP+GFLFNIV
Sbjct: 181 YYVNHPLYTPVSDLQMKIGFAFGVLCQVSNFYCHILLRNLRSPDGNGGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300
           TCANYTTEIYQW+GFNIATQT AGYVFLVVAA IMTNWALAKHRRLKKLFDGKEGRP+YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTIAGYVFLVVAAFIMTNWALAKHRRLKKLFDGKEGRPRYP 300

Query: 301 RRWVILPPFL 311
           RRWVILPPFL
Sbjct: 301 RRWVILPPFL 310

[Close]

BLAST of Cucsa.010800 vs. TAIR10
Match: AT3G55360.1 (AT3G55360.1 3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 561.2 bits (1445), Expect = 3.9e-160
Identity = 255/310 (82.26%), Postives = 284/310 (91.61%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60
           MKV+VVSRSGREV+K  + L DSA+VADLQ+A HKR KKFYP+RQRLTLPV PGSK++P 
Sbjct: 1   MKVTVVSRSGREVLKAPLDLPDSATVADLQEAFHKRAKKFYPSRQRLTLPVTPGSKDKPV 60

Query: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
           VLNSKKSL+EYC  N++S+TVVFKDLG QVSYRTLFFFEYLGPL++YP+FYYFPVY+F G
Sbjct: 61  VLNSKKSLKEYCDGNNNSLTVVFKDLGAQVSYRTLFFFEYLGPLLIYPVFYYFPVYKFLG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180
           Y  + VIHPVQTYAMYYWCFHYFKRI+ETFFVHRFSHATSP+ NVFRNCAYYWSFG+YIA
Sbjct: 121 YGEDCVIHPVQTYAMYYWCFHYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYIA 180

Query: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLYTPV DLQMKIGF FGL+CQV+NFYCHILL+ LR P G GGYQIP+GFLFNIV
Sbjct: 181 YYVNHPLYTPVSDLQMKIGFGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300
           TCANYTTEIYQW+GFNIATQT AGYVFL VAALIMTNWAL KH RL+K+FDGK+G+PKYP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTIAGYVFLAVAALIMTNWALGKHSRLRKIFDGKDGKPKYP 300

Query: 301 RRWVILPPFL 311
           RRWVILPPFL
Sbjct: 301 RRWVILPPFL 310

[Close]

BLAST of Cucsa.010800 vs. TAIR10
Match: AT5G16010.1 (AT5G16010.1 3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 77.0 bits (188), Expect = 2.2e-14
Identity = 57/210 (27.14%), Postives = 95/210 (45.24%), Query Frame = 1


Query: 102 GPLILYPIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSP 161
           G L+LY   +      FF   ++ +   +   A+     H+FKR+ E  F+H++S   + 
Sbjct: 68  GMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSAL---ALHFFKRVFEVLFIHKYSGGMA- 127

Query: 162 LSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDLQMKI-GFAFGLLCQVSNFYCHILLRKL 221
           + +     + Y+S  + + Y  N  L        MK+ G    ++  V N Y H+LL KL
Sbjct: 128 IDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLLAKL 187

Query: 222 RSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWAL 281
           R  +G   Y+IPKG LF+I+ C +Y  EI  +  F + +QT   + F +   L +   + 
Sbjct: 188 RKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIGRSY 247

Query: 282 AKHRRLKKLFDGKEGRPKYPRRWVILPPFL 311
           A        FD       +P+    L PF+
Sbjct: 248 ATRTWYLSKFD------DFPKHIKALIPFV 267

[Close]

BLAST of Cucsa.010800 vs. TAIR10
Match: AT2G38050.1 (AT2G38050.1 3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 52.0 bits (123), Expect = 7.7e-07
Identity = 33/115 (28.70%), Postives = 57/115 (49.57%), Query Frame = 1


Query: 200 FAFGLLCQVSNFYCHIL----LRKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGF 259
           F  G++  ++  Y +I     L +L+  E  GGY IP+G  F +V+C NY  E  +W+G+
Sbjct: 153 FVIGMVVFITGMYINITSDRTLVRLKK-ENRGGYVIPRGGWFELVSCPNYFGEAIEWLGW 212

Query: 260 NIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYPRRWVILPPFL 311
            + T ++AG  F +     +   A A H+     F     + +YP+    + PF+
Sbjct: 213 AVMTWSWAGIGFFLYTCSNLFPRARASHKWYIAKF-----KEEYPKTRKAVIPFV 261

[Close]

BLAST of Cucsa.010800 vs. NCBI nr
Match: gi|449450010|ref|XP_004142757.1| (PREDICTED: very-long-chain enoyl-CoA reductase [Cucumis sativus])

HSP 1 Score: 649.0 bits (1673), Expect = 4.1e-183
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60
           MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60

Query: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
           VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG
Sbjct: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180
           YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA
Sbjct: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180

Query: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV
Sbjct: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300
           TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP
Sbjct: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300

Query: 301 RRWVILPPFL 311
           RRWVILPPFL
Sbjct: 301 RRWVILPPFL 310

[Close]

BLAST of Cucsa.010800 vs. NCBI nr
Match: gi|659117972|ref|XP_008458883.1| (PREDICTED: very-long-chain enoyl-CoA reductase [Cucumis melo])

HSP 1 Score: 647.5 bits (1669), Expect = 1.2e-182
Identity = 309/310 (99.68%), Postives = 310/310 (100.00%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60
           MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60

Query: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
           VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG
Sbjct: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180
           Y+AERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA
Sbjct: 121 YQAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180

Query: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV
Sbjct: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300
           TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP
Sbjct: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300

Query: 301 RRWVILPPFL 311
           RRWVILPPFL
Sbjct: 301 RRWVILPPFL 310

[Close]

BLAST of Cucsa.010800 vs. NCBI nr
Match: gi|255580033|ref|XP_002530850.1| (PREDICTED: very-long-chain enoyl-CoA reductase [Ricinus communis])

HSP 1 Score: 596.3 bits (1536), Expect = 3.1e-167
Identity = 272/310 (87.74%), Postives = 299/310 (96.45%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60
           MKV+VVSRSGREV+KGG+ L+DSA+VADLQ+AIHKRTKKFYP+RQRLTLP+ PGSK+RPT
Sbjct: 1   MKVTVVSRSGREVIKGGLDLNDSATVADLQEAIHKRTKKFYPSRQRLTLPLPPGSKDRPT 60

Query: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
           VLN KKSL++YC  NSD+ITVVFKDLGPQVSYRTLFFFEYLGPLILYP+FY+FPVY++FG
Sbjct: 61  VLNYKKSLKDYCDGNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPVFYFFPVYKYFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180
           YK ERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYW+FGSYIA
Sbjct: 121 YKEERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIA 180

Query: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLYTPVGDLQMKIGF FGL+CQ++NFYCHILL+ LRSP+G+GGYQIP GFLFNIV
Sbjct: 181 YYVNHPLYTPVGDLQMKIGFGFGLICQIANFYCHILLKNLRSPDGSGGYQIPHGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300
           TCANYTTEIYQW+GFNIATQT AGY+FLVVAA IMTNWALAKHRRL+KLFDGK+GRPKYP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTVAGYIFLVVAASIMTNWALAKHRRLRKLFDGKDGRPKYP 300

Query: 301 RRWVILPPFL 311
           RRWVILPPFL
Sbjct: 301 RRWVILPPFL 310

[Close]

BLAST of Cucsa.010800 vs. NCBI nr
Match: gi|657981584|ref|XP_008382818.1| (PREDICTED: very-long-chain enoyl-CoA reductase [Malus domestica])

HSP 1 Score: 591.7 bits (1524), Expect = 7.7e-166
Identity = 271/310 (87.42%), Postives = 297/310 (95.81%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60
           MKV+VVSRSGREVVKGG++LSDSA+VADLQDAIHKRTKKFYPARQRLTLPVQPGSKERP 
Sbjct: 1   MKVTVVSRSGREVVKGGLELSDSATVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPV 60

Query: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
           VL+ KKSL++Y S NSD++TVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVY + G
Sbjct: 61  VLSYKKSLQDYISGNSDNLTVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYDYLG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180
           +K +RVIHPVQTYA+YYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFG++IA
Sbjct: 121 FKGDRVIHPVQTYALYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGAFIA 180

Query: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YY+NHPLYTPV DLQMKIGF  G++CQ+SNFYCHILLR LRSP+GNGGYQIP+GFLFNIV
Sbjct: 181 YYLNHPLYTPVSDLQMKIGFGIGIICQISNFYCHILLRNLRSPDGNGGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300
           TCANYTTEIYQW+GFNIATQT AGY+FL+VAA IMTNWALAKHRRLKK+FDGK+GRPKYP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTVAGYIFLIVAASIMTNWALAKHRRLKKIFDGKDGRPKYP 300

Query: 301 RRWVILPPFL 311
           RRWVILPPFL
Sbjct: 301 RRWVILPPFL 310

[Close]

BLAST of Cucsa.010800 vs. NCBI nr
Match: gi|224098417|ref|XP_002311166.1| (3-oxo-5-alpha-steroid 4-dehydrogenase family protein [Populus trichocarpa])

HSP 1 Score: 587.4 bits (1513), Expect = 1.4e-164
Identity = 269/310 (86.77%), Postives = 293/310 (94.52%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60
           MKV+VVSRSGREV+KGG++LSDSA+VADLQ+AIHKRTKKFYPARQRLTLP+ PGSKERPT
Sbjct: 1   MKVTVVSRSGREVIKGGLELSDSATVADLQEAIHKRTKKFYPARQRLTLPLPPGSKERPT 60

Query: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
           VLN KK+L+EY   N DS+T++FKDLGPQVSYRTLFFFEYLGPL+LYP FYYFPVYQF G
Sbjct: 61  VLNYKKNLKEYQDGNLDSLTILFKDLGPQVSYRTLFFFEYLGPLVLYPFFYYFPVYQFLG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180
           YK E ++HPVQTYA+YYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYW+FGSYIA
Sbjct: 121 YKGEHIMHPVQTYALYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIA 180

Query: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLYTPVGDLQMKIGF FGL+CQ++NFYCHILLR LR P+GNGGYQIP GFLFNIV
Sbjct: 181 YYVNHPLYTPVGDLQMKIGFGFGLVCQLANFYCHILLRNLRRPDGNGGYQIPSGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300
           TCANYTTEIYQW+GFNIATQT AGY+FLVVAA IMTNWALAKHRRLKKLFDGK+GRPKYP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTVAGYIFLVVAASIMTNWALAKHRRLKKLFDGKDGRPKYP 300

Query: 301 RRWVILPPFL 311
           RRWVILPP L
Sbjct: 301 RRWVILPPVL 310

The following BLAST results are available for this feature:

BLAST of Cucsa.010800 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TECR_ARATH	7.0e-159	82.26	Very-long-chain enoyl-CoA reductase OS=Arabidopsis thaliana GN=ECR PE=1 SV=1	[more]
TECR_DICDI	2.9e-56	39.35	Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum GN=gpsn2 PE=3 SV...	[more]
TECR_HUMAN	6.3e-51	38.61	Very-long-chain enoyl-CoA reductase OS=Homo sapiens GN=TECR PE=1 SV=1	[more]
TECR_BOVIN	1.4e-50	38.61	Very-long-chain enoyl-CoA reductase OS=Bos taurus GN=TECR PE=2 SV=1	[more]
TECR_CAEEL	3.1e-50	38.41	Probable very-long-chain enoyl-CoA reductase art-1 OS=Caenorhabditis elegans GN=...	[more]

BLAST of Cucsa.010800 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KNT8_CUCSA	2.8e-183	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G517090 PE=4 SV=1	[more]
B9SXZ3_RICCO	2.2e-167	87.74	Synaptic glycoprotein SC2, putative OS=Ricinus communis GN=RCOM_1206840 PE=4 SV=...	[more]
A9PDL9_POPTR	1.0e-164	86.77	3-oxo-5-alpha-steroid 4-dehydrogenase family protein OS=Populus trichocarpa GN=P...	[more]
M9T3Q3_MALDO	1.7e-164	86.45	Enoyl-ACP reductase OS=Malus domestica PE=2 SV=1	[more]
A0A059BI33_EUCGR	2.3e-164	87.42	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_G02931 PE=4 SV=1	[more]

BLAST of Cucsa.010800 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G55360.1	3.9e-160	82.26	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT5G16010.1	2.2e-14	27.14	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	7.7e-07	28.70	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

BLAST of Cucsa.010800 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449450010\|ref\|XP_004142757.1\|	4.1e-183	100.00	PREDICTED: very-long-chain enoyl-CoA reductase [Cucumis sativus]	[more]
gi\|659117972\|ref\|XP_008458883.1\|	1.2e-182	99.68	PREDICTED: very-long-chain enoyl-CoA reductase [Cucumis melo]	[more]
gi\|255580033\|ref\|XP_002530850.1\|	3.1e-167	87.74	PREDICTED: very-long-chain enoyl-CoA reductase [Ricinus communis]	[more]
gi\|657981584\|ref\|XP_008382818.1\|	7.7e-166	87.42	PREDICTED: very-long-chain enoyl-CoA reductase [Malus domestica]	[more]
gi\|224098417\|ref\|XP_002311166.1\|	1.4e-164	86.77	3-oxo-5-alpha-steroid 4-dehydrogenase family protein [Populus trichocarpa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001104	3-oxo-5_a-steroid_4-DH_C
IPR001104	3-oxo-5_a-steroid_4-DH_C

Vocabulary: **Molecular Function**
Term	Definition
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm
GO:0016021	integral component of membrane
GO:0005737	cytoplasm
GO:0016021	integral component of membrane

Vocabulary: **Biological Process**
Term	Definition
GO:0006629	lipid metabolic process
GO:0006629	lipid metabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042967	acyl-carrier-protein biosynthetic process
biological_process	GO:0009651	response to salt stress
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0008209	androgen metabolic process
biological_process	GO:0016126	sterol biosynthetic process
biological_process	GO:0046520	sphingoid biosynthetic process
biological_process	GO:0010025	wax biosynthetic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0007030	Golgi organization
biological_process	GO:0006633	fatty acid biosynthetic process
biological_process	GO:0008210	estrogen metabolic process
biological_process	GO:0006816	calcium ion transport
biological_process	GO:0006699	bile acid biosynthetic process
cellular_component	GO:0009923	fatty acid elongase complex
cellular_component	GO:0005835	fatty acid synthase complex
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0003865	3-oxo-5-alpha-steroid 4-dehydrogenase activity
molecular_function	GO:0004318	enoyl-[acyl-carrier-protein] reductase (NADH) activity
molecular_function	GO:0009922	fatty acid elongase activity
molecular_function	GO:0019166	trans-2-enoyl-CoA reductase (NADPH) activity
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.010800.1	Cucsa.010800.1	mRNA
Cucsa.010800.2	Cucsa.010800.2	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 130..280 score: 1.3
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROFILE	PS50244	S5A_REDUCTASE	coord: 170..251 score: 21
None	No IPR available	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 4..281 score: 4.1E
None	No IPR available	PANTHER	PTHR10556:SF28	SC2	coord: 4..281 score: 4.1E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.010800	Cla004664	Watermelon (97103) v1	cgywmB359
Cucsa.010800	Csa5G517090	Cucumber (Chinese Long) v2	cgycuB286
Cucsa.010800	MELO3C021634	Melon (DHL92) v3.5.1	cgymeB336
Cucsa.010800	ClCG09G019780	Watermelon (Charleston Gray)	cgywcgB338
Cucsa.010800	CSPI05G17720	Wild cucumber (PI 183967)	cgycpiB296
Cucsa.010800	CmaCh15G003870	Cucurbita maxima (Rimu)	cgycmaB0542
Cucsa.010800	CmaCh04G026440	Cucurbita maxima (Rimu)	cgycmaB0543
Cucsa.010800	CmoCh15G003980	Cucurbita moschata (Rifu)	cgycmoB0543
Cucsa.010800	CmoCh04G027660	Cucurbita moschata (Rifu)	cgycmoB0544
Cucsa.010800	Lsi02G016350	Bottle gourd (USVL1VR-Ls)	cgylsiB318
Cucsa.010800	Cp4.1LG13g09400	Cucurbita pepo (Zucchini)	cgycpeB0519
Cucsa.010800	Cp4.1LG01g23900	Cucurbita pepo (Zucchini)	cgycpeB0522
Cucsa.010800	MELO3C021634.2	Melon (DHL92) v3.6.1	cgymedB331
Cucsa.010800	CsaV3_5G026640	Cucumber (Chinese Long) v3	cgycucB300
Cucsa.010800	Cla97C09G181210	Watermelon (97103) v2	cgywmbB348
Cucsa.010800	Bhi06G000532	Wax gourd	cgywgoB388
Cucsa.010800	Carg02349	Silver-seed gourd	carcgyB0315
Cucsa.010800	Carg04501	Silver-seed gourd	carcgyB0766

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Cucsa.010800	Cucsa.066140	Cucumber (Gy14) v1	cgycgyB017

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.010800	Watermelon (97103) v2	cgywmbB347