Cp4.1LG01g23900 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG01g23900
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Trans-2,3-enoyl-CoA reductase
Location	Cp4.1LG01 : 19391559 .. 19395980 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAGAAAGCCCAAAGCAATATATATAATATATACTAAGGGCTGTTGTTCTGAACCCCATTGATCCGAGGAGATAGTCAAATTAGAAGACAGTAGGCAGAAAGTATAAGATGTGCCTTCATCAAACAGGTTGCAGGCATTGTCAAGTTAAATTGCACACGGCACACACACCTAATTTCTGAAAAAAGAAGAAGCTGAAATTGATATCTGATTTCAAGAACATAGCTAGACAACTGTTGATAATATCATTGATCAGAAACAGAAATGGAAGTCCTATACTCAAATAAGAAGACAATAGGCAAAAAATAAAAGGTGAACATTTCATCAAACAGGTTATAGGCATAATCAAGATAAATTGCACCCAGCAATCGTCACATACATGACCATATTTCCGACTATGAATAATCATCAGAGAGTGACTCTACGTATTTAAGAACATAACAGATTGAGAGAATACAAGATTTGGTTTGTAGATGTTGTGGACGACGGAGGCGCCGGCGAGAAGCGAAGAGATGACGACGACGAAGGACCAACCCACCGAGGGGGTTCCAGTGGGGAGCAACCGACCACCGAATTTCTGAGCCATTATTCAGCTCAACACCGTACAAAGATATCAATTTCTCACCCTAAAAAGGTTTCATTATTCAATCTGCCCGCCAACTGCCCTTTAAATAACCGGTCCAAGGCTCCGGCCCGGCTCCAGCTTCGCAAACCCGTTGTTTTTTTTTTTTTTTTTGTTACATTTTCGATTTTGGGTTAAATATTCAATTTAGTATGTTTTAACATTTTGAATGCGTCGAATTGTAAATTTGTACTGAGGAGATGACTAGGAGGAACGCGCTGCATCCGTCGTTGGAATTAGCTCTCCTACCCCAACTGCACGCCATTTAATAAGCCTACTTCCCAACCTCCCCCTTCAAAACCCACTTCAAACTCAGAGAACTTTCGCCATTTTTTAATAAAATCCCTTAGCAGATCTCAGCGTATAACCATTTCTGGGTTCTGGGTCCTCTGATTCTGCTATTTCAGGATCTGGGTTGAAATCACAGTGGGGTTAATTTGGCTGTGAAGATGAAGGTCTCAGTAGTTTCTCGCAGTGGGAGAGAAGTTGTTGACGGCGGCATTCAACTAAGCGATTCTGTAAGTGTTCGGATTTTGATTCAATTACTGTTTCTTTCTCGATTCTTGGAATACAAGAAAAGATGATAAGAGTACAACTTGTTTTGGTGGCTGTGTTCTNTGAGTAGCTGATGGGATCTTAAGTTTCATGGACATTGTTGTTTAATCCTTTGCAGGCTTCGGTGGCGGATCTGCAGGATGCGATTTACAACCGAAGTGAGTACATGAGGGTAGAATGTTTTCTTTAATTGTTGCATACATGTTCTTTTTAGCTTAGAATTGTTAGAAATCTTAGCTTAGATTTGACATTTTTGTTGGAGTGAGTGATAGAACTGGACTTGTTGTTAAACGGATGAATCTAAAGATTAAGTTTAGCCTAGCACAACAGTTTAAGCTTTTGGGTGTGAAACTGTAACAGGTATGCAGACATATTGTCATGTCCTCTTTGAGCTTCACTTTTCGGGCTTTGGCTCAAGGTTTTAAACGTGTCGGCTNGCTATGCAGATATTGTCATGTCCTCTTTGAGCTTCACTTTTCGGGCTTTGGCTCACTTTTCGGGCTTTGGCTCAAGGTTTTAAACGTGTCGGCTAGGGAGAGGTTTTCACATCCTTATAAAGAATGCTTCGTTCTCCTTCCAACCAATGTGCGATCTCACAATTCACCCCCATTTGGGCCAACGAATCTTACAATCCACCTCCGTTTTCCTCCCCAACCGACGTGGAATCTCAAAATCCACCCCCTTCAAGGCTCAGCGTCTTTGTTGACATTCTTTCCCTTCTTCAATAGATGAGGGACCCCAATCCACCCTCTATGGGGCCCGACGTCCTTGCTGGAAGACCGCCTCGTTTCCACCTCCTTCGGAGCTCAACCTCCTCACTGGCGCATCACCCCAGTGTCTGGCTCTAATACTATTTGTAACAGTCCAAACCTACTGGATATTGTCCTCTCTGGACTTTCCCTTCAAAGCTTCCCTCCAAANCGAATCTTACAATCCACCTCCGTTTTCCTCCCCAACCGATGTGGAATCTCACAATCCACCCCCCTTCAAGGCTCAACGTCTTTGTTGACATTCTTTCCCTTCTCCAATAGATGAGAGACCCCAATCCACCCTCTATGGGGCCCGACGTCCTTGCTGGAAGACCGCCTCGTTTCCACCCCCCTTCGGAGCTCAACCTCCTCACTGGCGCATCGCCCTGGTGTCTGGCTTTAATACTATTTGTAACAGTCCAAACTTACTGGATATTGTCCTCTTTGGACTTTTCCTTCAAAGCTTCCATTCAAAGTTTTAAGACGCATAGGAAGANAAGTTTTAAGACGCATCGACTAGGAAGAGGTTTCTACTCTTTTATAAAGAATGTTTTGCAAAAATTGCACAAAATATGTTTCACTACTTCATAATAAAGTATTTCTATCTTNGCTCAACGTCTTTGTTGACATTCTTTCCCTTCTCCAATAGATGAGAGACCCCAATCCACCCTCTATGGGGCCCGACGTCCTTGCTGGAAGACCGCCTCGTTTCCACCCCCCTTCGGAGCTCAACCTCCTCACTGGCGCATCGCCCTGGTGTCTGGCTTTAATACTATTTGTAACAGTCCAAACTTACTGGATATTGTCCTCTTTGGACTTTTCCTTCAAAGCTTCCATTCAAAGTTTTAAGACGCATAGGAAGAGGTTTCTACTCTTTTATAAAGAATGTTTTGCAAAAACTGCACGGCTGACAGGTGAGGAATCAAAATATGTTTCACTACTTCATAATAAAGTATTTCTATCTTATCCTTCCTGAAAATTAGTTCTTTCATTACTGTAAAATGGTTAAATGGTTCTTAAGCAGTAGCAAATTGGTAGTCTTTTAGCATGCATACATGTAAGTCGATATCAATTGTTATGTAAAGTGCAGCTACCTGATTTCGTTGTTTCATTGAAACAGCCAAAAAGTTCTACCCTGCTAGGCAGCGGTTAACTCTTCCAGTTCAACCTGGTTCAAAGGAGAGGCCTACTAGAGAGTATTGTAGCAACAACTCAGACAACATAACTGTTGTCTTCAAGGACTTGGGTCCACAAGTTTCTTATCGTACTCTCTTCTTTTTTGAATACTTGGGCCCCTTGATCCTCTACCCCATCTTTTACTACTTCCCTGTCTATCAGTTCTTTGGCTACAAGGCCGAACGTGTCATCCATCCGGTTCAGACGTATGCAATGTACTACTGGTGTTTTCACTACTTCAAACGAATCATGGAAACATTTTTCGTTTCGTATATTGCATATTATGTTAATCACCCACTTTATAGCCCTGTTGGTGACCTTCAAATGAAGATTGGGTTTGCATTTGGTTTGCTGTGTCAAGTGTGTAACTTCTACTGTCATATTTTGCTGAGAAAACTGCGGAGCCCTGAGGGGAATGGGGGATATCAAATCCCAAAGGGGTTTCTGTTCAACATTGTGACATGTGCAAACTACACAACAGAAATATATCAGTGGGTAGGCTTCAATATAGCTACACAGACTGTCGCTGGTTACATCTTTCTTGTGGTTGCTACTCTGATCATGACGAACTGGGCCCTTGCAAAGCACCGTCGCCTCAAGAAGGTATAGTATATCTAACTTATTTCTCTTAGTTGTTCTTAAGGTTAGGAATCACGAACCTCCACAATGGTATGATATTGTCCACTTTGAACATGTAGTTTTGCTTTTGATTACTCCAGAAGACCCATGGAGATAGTATTCCTTACTTATACACCTATGATCGACCCCTTACTTACTCATGTTTATTTTTCTTTCACATAATGACTTTTCCGTCCCTATGTGNGAAATCACGAACCTCCACGATGGTATGATATTGTTCACTTTGAACATGTAGTTTTGCTTTTGGTTACTCCAGAAGACTAATGGAGATAGTATTCCTTACTTATACACCTATGATCGACCCCTTACTTACTCACGTTTATTTTTCTTTCACATAATGACTTTTCCGTCCCTATGTGCAGCTGTTTGATGGGAAGGAGGGAAGGCCAAGGTACCCACGCAGATGGGTGATACTTCCTCCATTTCTGTAGAAGGATTGATTGGGCGGCCTCAGATCCATATTTTATCGAGTTGGTAGCTCTAAGTTTAAAATGTCCCTTGAAGAACACCACAAGATATCATTTTCTCAATGTATTTGATGTTCTTTGAGGTTGTCTTAACAGTACTTTGCCTTGTTCTTTGTGTTGTATGGGAATCAAAATAAATTATTATTTATTGGGACATTTTTATTAGCTGATTGATATATCATAATTTTGAAGTATAATTAATTTATTCAAATTAA

mRNA sequence

AAGAGAAAGCCCAAAGCAATATATATAATATATACTAAGGGCTGTTGTTCTGAACCCCATTGATCCGAGGAGATAGTCAAATTAGAAGACAGTAGGCAGAAAGTATAAGATGTGCCTTCATCAAACAGGTTGCAGGCATTGTCAAGTTAAATTGCACACGGCACACACACCTAATTTCTGAAAAAAGAAGAAGCTGAAATTGATATCTGATTTCAAGAACATAGCTAGACAACTGTTGATAATATCATTGATCAGAAACAGAAATGGAAGTCCTATACTCAAATAAGAAGACAATAGGCAAAAAATAAAAGGTGAACATTTCATCAAACAGGTTATAGGCATAATCAAGATAAATTGCACCCAGCAATCGTCACATACATGACCATATTTCCGACTATGAATAATCATCAGAGAGTGACTCTACGTATTTAAGAACATAACAGATTGAGAGAATACAAGATTTGGTTTGTAGATGTTGTGGACGACGGAGGCGCCGGCGAGAAGCGAAGAGATGACGACGACGAAGGACCAACCCACCGAGGGGGTTCCAGTGGGGAGCAACCGACCACCGAATTTCTGAGCCATTATTCAGCTCAACACCGTACAAAGATATCAATTTCTCACCCTAAAAAGGTTTCATTATTCAATCTGCCCGCCAACTGCCCTTTAAATAACCGGTCCAAGGCTCCGGCCCGGCTCCAGCTTCGCAAACCCGTTGTTTTTTTTTTTTTTTTTGTTACATTTTCGATTTTGGGTTAAATATTCAATTTAGTATGTTTTAACATTTTGAATGCGTCGAATTGTAAATTTGTACTGAGGAGATGACTAGGAGGAACGCGCTGCATCCGTCGTTGGAATTAGCTCTCCTACCCCAACTGCACGCCATTTAATAAGCCTACTTCCCAACCTCCCCCTTCAAAACCCACTTCAAACTCAGAGAACTTTCGCCATTTTTTAATAAAATCCCTTAGCAGATCTCAGCGTATAACCATTTCTGGGTTCTGGGTCCTCTGATTCTGCTATTTCAGGATCTGGGTTGAAATCACAGTGGGGTTAATTTGGCTGTGAAGATGAAGGTCTCAGTAGTTTCTCGCAGTGGGAGAGAAGTTGTTGACGGCGGCATTCAACTAAGCGATTCTGCTTCGGTGGCGGATCTGCAGGATGCGATTTACAACCGAACCAAAAAGTTCTACCCTGCTAGGCAGCGGTTAACTCTTCCAGTTCAACCTGGTTCAAAGGAGAGGCCTACTAGAGAGTATTGTAGCAACAACTCAGACAACATAACTGTTGTCTTCAAGGACTTGGGTCCACAAGTTTCTTATCGTACTCTCTTCTTTTTTGAATACTTGGGCCCCTTGATCCTCTACCCCATCTTTTACTACTTCCCTGTCTATCAGTTCTTTGGCTACAAGGCCGAACGTGTCATCCATCCGGTTCAGACGTATGCAATGTACTACTGGTGTTTTCACTACTTCAAACGAATCATGGAAACATTTTTCGTTTCGTATATTGCATATTATGTTAATCACCCACTTTATAGCCCTGTTGGTGACCTTCAAATGAAGATTGGGTTTGCATTTGGTTTGCTGTGTCAAGTGTGTAACTTCTACTGTCATATTTTGCTGAGAAAACTGCGGAGCCCTGAGGGGAATGGGGGATATCAAATCCCAAAGGGGTTTCTGTTCAACATTGTGACATGTGCAAACTACACAACAGAAATATATCAGTGGGTAGGCTTCAATATAGCTACACAGACTGTCGCTGGTTACATCTTTCTTGTGGTTGCTACTCTGATCATGACGAACTGGGCCCTTGCAAAGCACCGTCGCCTCAAGAAGCTGTTTGATGGGAAGGAGGGAAGGCCAAGGTACCCACGCAGATGGGTGATACTTCCTCCATTTCTGTAGAAGGATTGATTGGGCGGCCTCAGATCCATATTTTATCGAGTTGGTAGCTCTAAGTTTAAAATGTCCCTTGAAGAACACCACAAGATATCATTTTCTCAATGTATTTGATGTTCTTTGAGGTTGTCTTAACAGTACTTTGCCTTGTTCTTTGTGTTGTATGGGAATCAAAATAAATTATTATTTATTGGGACATTTTTATTAGCTGATTGATATATCATAATTTTGAAGTATAATTAATTTATTCAAATTAA

Coding sequence (CDS)

ATGAAGGTCTCAGTAGTTTCTCGCAGTGGGAGAGAAGTTGTTGACGGCGGCATTCAACTAAGCGATTCTGCTTCGGTGGCGGATCTGCAGGATGCGATTTACAACCGAACCAAAAAGTTCTACCCTGCTAGGCAGCGGTTAACTCTTCCAGTTCAACCTGGTTCAAAGGAGAGGCCTACTAGAGAGTATTGTAGCAACAACTCAGACAACATAACTGTTGTCTTCAAGGACTTGGGTCCACAAGTTTCTTATCGTACTCTCTTCTTTTTTGAATACTTGGGCCCCTTGATCCTCTACCCCATCTTTTACTACTTCCCTGTCTATCAGTTCTTTGGCTACAAGGCCGAACGTGTCATCCATCCGGTTCAGACGTATGCAATGTACTACTGGTGTTTTCACTACTTCAAACGAATCATGGAAACATTTTTCGTTTCGTATATTGCATATTATGTTAATCACCCACTTTATAGCCCTGTTGGTGACCTTCAAATGAAGATTGGGTTTGCATTTGGTTTGCTGTGTCAAGTGTGTAACTTCTACTGTCATATTTTGCTGAGAAAACTGCGGAGCCCTGAGGGGAATGGGGGATATCAAATCCCAAAGGGGTTTCTGTTCAACATTGTGACATGTGCAAACTACACAACAGAAATATATCAGTGGGTAGGCTTCAATATAGCTACACAGACTGTCGCTGGTTACATCTTTCTTGTGGTTGCTACTCTGATCATGACGAACTGGGCCCTTGCAAAGCACCGTCGCCTCAAGAAGCTGTTTGATGGGAAGGAGGGAAGGCCAAGGTACCCACGCAGATGGGTGATACTTCCTCCATTTCTGTAG

Protein sequence

MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPTREYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIMETFFVSYIAYYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYPRRWVILPPFL

Homology

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BLAST of Cp4.1LG01g23900 vs. Swiss-Prot
Match: TECR_ARATH (Very-long-chain enoyl-CoA reductase OS=Arabidopsis thaliana GN=ECR PE=1 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 8.2e-127
Identity = 217/310 (70.00%), Postives = 250/310 (80.65%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKV+VVSRSGREV+   + L DSA+VADLQ+A + R KKFYP+RQRLTLPV PGSK++P 
Sbjct: 1   MKVTVVSRSGREVLKAPLDLPDSATVADLQEAFHKRAKKFYPSRQRLTLPVTPGSKDKPV 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   +EYC  N++++TVVFKDLG QVSYRTLFFFEYLGPL++YP+FYYFPVY+F G
Sbjct: 61  VLNSKKSLKEYCDGNNNSLTVVFKDLGAQVSYRTLFFFEYLGPLLIYPVFYYFPVYKFLG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           Y  + VIHPVQTYAMYYWCFHYFKRI+ETFFV                        +YIA
Sbjct: 121 YGEDCVIHPVQTYAMYYWCFHYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLY+PV DLQMKIGF FGL+CQV NFYCHILL+ LR P G GGYQIP+GFLFNIV
Sbjct: 181 YYVNHPLYTPVSDLQMKIGFGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 279
           TCANYTTEIYQW+GFNIATQT+AGY+FL VA LIMTNWAL KH RL+K+FDGK+G+P+YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTIAGYVFLAVAALIMTNWALGKHSRLRKIFDGKDGKPKYP 300

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BLAST of Cp4.1LG01g23900 vs. Swiss-Prot
Match: TECR_DICDI (Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum GN=gpsn2 PE=3 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 6.7e-36
Identity = 106/309 (34.30%), Postives = 158/309 (51.13%), Query Frame = 1


Query: 3   VSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPTR- 62
           + VVS+  ++ V G    S S +V +L+  I ++T+      +R+ L V   + + P   
Sbjct: 4   IKVVSQRSKKEV-GSFSTSSSTTVGELKKQISSKTRL---GTERIRLAVPSKTSKLPNAF 63

Query: 63  EYCSNNSDNI--------TVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFGY 122
           E    +SD +        T+ FKDLGPQ+S+  +F  EY GPL +YPIFY+      +G 
Sbjct: 64  EALGKDSDLVSKHVGADSTLYFKDLGPQISWSLVFICEYAGPLFVYPIFYFLSNL-IYGT 123

Query: 123 KAERVIHPVQTYAMYYWCFHYFKRIMET------------FF------------VSYIAY 182
            + +     Q  A+  +  HY KRI ET             F             + ++Y
Sbjct: 124 DSPKSF--AQKVALVCYSLHYIKRIYETIFVHRFSHGTMPIFNLFKNCSYYWGCTAMVSY 183

Query: 183 YVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIVT 242
           +VNHPLY+     ++ +G    ++ +V N+ CHI LR LR P G+   +IP+G LF  V+
Sbjct: 184 FVNHPLYTEAPIERVYLGLGLWIIGEVFNYICHIQLRNLR-PAGSTERKIPRGLLFEFVS 243

Query: 243 CANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYPR 279
           C NYT EI  W+GF+I TQT+  +IF ++    M  WA+ KHRR +K F  K     YP+
Sbjct: 244 CPNYTVEILSWIGFSILTQTLTSWIFALMGAAQMWIWAVGKHRRYRKEFGDK-----YPK 299

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BLAST of Cp4.1LG01g23900 vs. Swiss-Prot
Match: TECR_CAEEL (Probable very-long-chain enoyl-CoA reductase art-1 OS=Caenorhabditis elegans GN=art-1 PE=3 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 1.8e-33
Identity = 93/276 (33.70%), Postives = 133/276 (48.19%), Query Frame = 1


Query: 38  KKFYPARQRLTLPVQPGSKERPTREYCSN---NSDNITVVFKDLGPQVSYRTLFFFEYLG 97
           KK     +R  L V+P  K     +  S+   +S    +  +DLGPQ++++T+F  EY G
Sbjct: 39  KKLKLTEERQALRVEPKGKPLADDQKLSDLGLSSQKAVLYVRDLGPQIAWKTVFMAEYAG 98

Query: 98  PLILYPIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIMETFFV---------- 157
           PL +YP+FY  P + +        +HP    A + W FHY KR+ ET F+          
Sbjct: 99  PLFVYPLFYLRPTFIYGQAAVNATMHPAVQIAFFAWSFHYAKRLFETQFIHRFGNSTMPQ 158

Query: 158 --------------SYIAYYVNHPLYSP--VGDLQMKIGFAFGLLCQVCNFYCHILLRKL 217
                         +++AY+VNHPL++P   GDLQ+  G A  ++ +  N   HILLR L
Sbjct: 159 FNLVKNCSYYWGFAAFVAYFVNHPLFTPPAFGDLQVYFGLAGFVISEFGNLSIHILLRNL 218

Query: 218 RSPEGNGGYQIPK------GFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLI 277
           R P G    +IPK        LFN V+C NYT E+  W+ F+I  Q++   IF       
Sbjct: 219 R-PAGTRERRIPKPDGNPLSLLFNYVSCPNYTYEVASWIFFSIMVQSLPAIIFTTAGFAQ 278

Query: 278 MTNWALAKHRRLKKLFDGKEGRPRYPRRWVILPPFL 279
           M  WA  KHR   K F      P YP+    + PF+
Sbjct: 279 MAIWAQGKHRNYLKEF------PDYPKNRKAIVPFV 307

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BLAST of Cp4.1LG01g23900 vs. Swiss-Prot
Match: TECR_HUMAN (Very-long-chain enoyl-CoA reductase OS=Homo sapiens GN=TECR PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 7.1e-30
Identity = 102/309 (33.01%), Postives = 154/309 (49.84%), Query Frame = 1


Query: 2   KVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTL-PVQPGSKERPT 61
           +V ++    RE +    ++   A++A++++       ++YPARQ L L P     K+   
Sbjct: 5   EVEILDAKTREKLCFLDKVEPHATIAEIKNLFTKTHPQWYPARQSLRLDPKGKSLKDEDV 64

Query: 62  REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFGYKAERVI- 121
            +     +   T+ F+DLG Q+S+ T+F  EY GPL +Y + +YF V   +G+K +    
Sbjct: 65  LQKLPVGT-TATLYFRDLGAQISWVTVFLTEYAGPLFIY-LLFYFRVPFIYGHKYDFTSS 124

Query: 122 -HPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIAYYVNHP 181
            H V   A     FHY KR++ET FV                        +++AYY+NHP
Sbjct: 125 RHTVVHLACICHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHP 184

Query: 182 LYSP--VGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPK------GFLFN 241
           LY+P   G  Q+K+  A  ++CQ+ NF  H+ LR LR P G+   +IP        +LF 
Sbjct: 185 LYTPPTYGAQQVKLALAIFVICQLGNFSIHMALRDLR-PAGSKTRKIPYPTKNPFTWLFL 244

Query: 242 IVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPR 276
           +V+C NYT E+  W+GF I TQ +   +F +V    MT WA  KHR   K F     R  
Sbjct: 245 LVSCPNYTYEVGSWIGFAIMTQCLPVALFSLVGFTQMTIWAKGKHRSYLKEF-----RDY 304

[Close]

BLAST of Cp4.1LG01g23900 vs. Swiss-Prot
Match: TECR_BOVIN (Very-long-chain enoyl-CoA reductase OS=Bos taurus GN=TECR PE=2 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.6e-29
Identity = 102/309 (33.01%), Postives = 153/309 (49.51%), Query Frame = 1


Query: 2   KVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTL-PVQPGSKERPT 61
           +V ++    RE +    ++   A++A++++       ++YPARQ L L P     K+   
Sbjct: 5   EVEILDAKTREKLCFLDKVEPQATIAEIKNLFTKTHPQWYPARQSLRLDPKGKSLKDEDV 64

Query: 62  REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFGYKAERVI- 121
            +     +   T+ F+DLG Q+S+ T+F  EY GPL +Y + +YF V   +G K +    
Sbjct: 65  LQKLPVGT-TATLYFRDLGAQISWVTVFLTEYAGPLFIY-LLFYFRVPFIYGRKYDFTSS 124

Query: 122 -HPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIAYYVNHP 181
            H V   A     FHY KR++ET FV                        +++AYY+NHP
Sbjct: 125 RHTVVHLACICHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHP 184

Query: 182 LYSP--VGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPK------GFLFN 241
           LY+P   G  Q+K+  A  ++CQ+ NF  H+ LR LR P G+   +IP        +LF 
Sbjct: 185 LYTPPTYGAQQVKLALAIFVICQLGNFSIHMALRDLR-PAGSKTRKIPYPTRNPFTWLFL 244

Query: 242 IVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPR 276
           +V+C NYT E+  W+GF I TQ +   +F +V    MT WA  KHR   K F     R  
Sbjct: 245 LVSCPNYTYEVGSWIGFAIMTQCLPVALFSLVGFTQMTIWAKGKHRSYLKEF-----RDY 304

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BLAST of Cp4.1LG01g23900 vs. TrEMBL
Match: A0A0A0KNT8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G517090 PE=4 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 3.4e-148
Identity = 267/310 (86.13%), Postives = 273/310 (88.06%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKVSVVSRSGREVV GGIQLSDSASVADLQDAI+ RTKKFYPARQRLTLPVQPGSKERPT
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   REYCS+NSD+ITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG
Sbjct: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV                        SYIA
Sbjct: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLY+PVGDLQMKIGFAFGLLCQV NFYCHILLRKLRSPEGNGGYQIPKGFLFNIV
Sbjct: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 279
           TCANYTTEIYQWVGFNIATQT AGY+FLVVA LIMTNWALAKHRRLKKLFDGKEGRP+YP
Sbjct: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. TrEMBL
Match: B9SXZ3_RICCO (Synaptic glycoprotein SC2, putative OS=Ricinus communis GN=RCOM_1206840 PE=4 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 4.7e-137
Identity = 239/310 (77.10%), Postives = 265/310 (85.48%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKV+VVSRSGREV+ GG+ L+DSA+VADLQ+AI+ RTKKFYP+RQRLTLP+ PGSK+RPT
Sbjct: 1   MKVTVVSRSGREVIKGGLDLNDSATVADLQEAIHKRTKKFYPSRQRLTLPLPPGSKDRPT 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   ++YC  NSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYP+FY+FPVY++FG
Sbjct: 61  VLNYKKSLKDYCDGNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPVFYFFPVYKYFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           YK ERVIHPVQTYAMYYWCFHYFKRIMETFFV                        SYIA
Sbjct: 121 YKEERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLY+PVGDLQMKIGF FGL+CQ+ NFYCHILL+ LRSP+G+GGYQIP GFLFNIV
Sbjct: 181 YYVNHPLYTPVGDLQMKIGFGFGLICQIANFYCHILLKNLRSPDGSGGYQIPHGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 279
           TCANYTTEIYQW+GFNIATQTVAGYIFLVVA  IMTNWALAKHRRL+KLFDGK+GRP+YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTVAGYIFLVVAASIMTNWALAKHRRLRKLFDGKDGRPKYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. TrEMBL
Match: A0A061FMS3_THECC (3-oxo-5-alpha-steroid 4-dehydrogenase family protein isoform 1 OS=Theobroma cacao GN=TCM_042773 PE=4 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 3.3e-135
Identity = 236/310 (76.13%), Postives = 261/310 (84.19%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKV++VSRSGREV+ GG++L DSA+VADLQ+AI+ RTKKFYP+RQRLTLPV PGSKERP 
Sbjct: 1   MKVTLVSRSGREVIKGGLELHDSATVADLQEAIHKRTKKFYPSRQRLTLPVPPGSKERPV 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   ++YC  N +NITVVFKDLGPQVSY TLFFFEYLGPLILYP FYYFPVYQFFG
Sbjct: 61  VLNYKKGLKDYCDGNQNNITVVFKDLGPQVSYPTLFFFEYLGPLILYPTFYYFPVYQFFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           YK ERVIHPVQTYA+YYWCFHYFKRIMETFF+                        +YIA
Sbjct: 121 YKVERVIHPVQTYALYYWCFHYFKRIMETFFIHRFSHATSPLSNVFRNCAYYWTFGAYIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLY+PV DLQMKIGF FGL+CQ+ NFYCHI+L+ LRSP+G+GGYQIP GFLFNIV
Sbjct: 181 YYVNHPLYTPVSDLQMKIGFGFGLICQLANFYCHIILKNLRSPDGSGGYQIPSGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 279
           TCANYTTEIYQW+GFNIATQTVAGY+FLVVAT IMTNWALAKHRRLKKLFDGKEGRP+YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTVAGYVFLVVATSIMTNWALAKHRRLKKLFDGKEGRPKYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. TrEMBL
Match: M9T3Q3_MALDO (Enoyl-ACP reductase OS=Malus domestica PE=2 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 3.3e-135
Identity = 236/310 (76.13%), Postives = 262/310 (84.52%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKV+VVSRSGREVV GG++L+DSA+VADLQDAIY RTKKFYPARQRLTLPVQPGSKERP 
Sbjct: 1   MKVTVVSRSGREVVKGGLELTDSATVADLQDAIYKRTKKFYPARQRLTLPVQPGSKERPV 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   ++Y S NSDN+TVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVY + G
Sbjct: 61  VLSYKKSLQDYISGNSDNLTVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYDYLG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           +K +RVIHPVQTYA+YYWCFHYFKRIMETFFV                        ++IA
Sbjct: 121 FKGDRVIHPVQTYALYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGAFIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YY+NHPLY+PV DLQMKIGF FG++CQ+ N YCHILLR LRSP+GNGGYQIP+GFLFNIV
Sbjct: 181 YYLNHPLYTPVSDLQMKIGFGFGIICQISNLYCHILLRNLRSPDGNGGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 279
           TCANYTTEIYQW+GFNIATQTVAGYIFL+VA  IMTNWAL KHRRLKK+FDGK+GRP+YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTVAGYIFLIVAASIMTNWALTKHRRLKKIFDGKDGRPKYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. TrEMBL
Match: M9T617_MALDO (Enoyl-ACP reductase OS=Malus domestica PE=2 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 9.7e-135
Identity = 235/310 (75.81%), Postives = 262/310 (84.52%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKV+VVSRSGREVV GG++L+DSA+VADLQDAIY RTKKFYPARQRLTLPVQPGSKERP 
Sbjct: 1   MKVTVVSRSGREVVKGGLELTDSATVADLQDAIYKRTKKFYPARQRLTLPVQPGSKERPV 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   ++Y S NSDN+TVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVY + G
Sbjct: 61  VLSYKKSLQDYISGNSDNLTVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYDYLG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           +K +RVIHPVQTYA+YYWCFHYFKRIMETFFV                        ++IA
Sbjct: 121 FKGDRVIHPVQTYALYYWCFHYFKRIMETFFVHHFSHATSPLSNVFRNCAYYWSFGAFIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YY+NHPLY+PV DLQMKIGF FG++CQ+ N YCHILLR LRSP+GNGGYQIP+GFLFNIV
Sbjct: 181 YYLNHPLYTPVSDLQMKIGFGFGIICQISNLYCHILLRNLRSPDGNGGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 279
           TCANYTTEIYQW+GFNIATQTVAG+IFL+VA  IMTNWAL KHRRLKK+FDGK+GRP+YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTVAGFIFLIVAASIMTNWALTKHRRLKKIFDGKDGRPKYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. TAIR10
Match: AT3G55360.1 (AT3G55360.1 3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 454.5 bits (1168), Expect = 4.6e-128
Identity = 217/310 (70.00%), Postives = 250/310 (80.65%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKV+VVSRSGREV+   + L DSA+VADLQ+A + R KKFYP+RQRLTLPV PGSK++P 
Sbjct: 1   MKVTVVSRSGREVLKAPLDLPDSATVADLQEAFHKRAKKFYPSRQRLTLPVTPGSKDKPV 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   +EYC  N++++TVVFKDLG QVSYRTLFFFEYLGPL++YP+FYYFPVY+F G
Sbjct: 61  VLNSKKSLKEYCDGNNNSLTVVFKDLGAQVSYRTLFFFEYLGPLLIYPVFYYFPVYKFLG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           Y  + VIHPVQTYAMYYWCFHYFKRI+ETFFV                        +YIA
Sbjct: 121 YGEDCVIHPVQTYAMYYWCFHYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLY+PV DLQMKIGF FGL+CQV NFYCHILL+ LR P G GGYQIP+GFLFNIV
Sbjct: 181 YYVNHPLYTPVSDLQMKIGFGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 279
           TCANYTTEIYQW+GFNIATQT+AGY+FL VA LIMTNWAL KH RL+K+FDGK+G+P+YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTIAGYVFLAVAALIMTNWALGKHSRLRKIFDGKDGKPKYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. TAIR10
Match: AT5G16010.1 (AT5G16010.1 3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 57.4 bits (137), Expect = 1.6e-08
Identity = 34/103 (33.01%), Postives = 51/103 (49.51%), Query Frame = 1


Query: 176 VCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIF 235
           V N Y H+LL KLR  +G   Y+IPKG LF+I+ C +Y  EI  +  F + +QT+  + F
Sbjct: 171 VGNLYHHVLLAKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSF 230

Query: 236 LVVATLIMTNWALAKHRRLKKLFDGKEGRPRYPRRWVILPPFL 279
            +   L +   + A        FD       +P+    L PF+
Sbjct: 231 AMGTMLYLIGRSYATRTWYLSKFDD------FPKHIKALIPFV 267

[Close]

BLAST of Cp4.1LG01g23900 vs. NCBI nr
Match: gi|449450010|ref|XP_004142757.1| (PREDICTED: very-long-chain enoyl-CoA reductase [Cucumis sativus])

HSP 1 Score: 532.3 bits (1370), Expect = 4.9e-148
Identity = 267/310 (86.13%), Postives = 273/310 (88.06%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKVSVVSRSGREVV GGIQLSDSASVADLQDAI+ RTKKFYPARQRLTLPVQPGSKERPT
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   REYCS+NSD+ITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG
Sbjct: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV                        SYIA
Sbjct: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLY+PVGDLQMKIGFAFGLLCQV NFYCHILLRKLRSPEGNGGYQIPKGFLFNIV
Sbjct: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 279
           TCANYTTEIYQWVGFNIATQT AGY+FLVVA LIMTNWALAKHRRLKKLFDGKEGRP+YP
Sbjct: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. NCBI nr
Match: gi|659117972|ref|XP_008458883.1| (PREDICTED: very-long-chain enoyl-CoA reductase [Cucumis melo])

HSP 1 Score: 530.8 bits (1366), Expect = 1.4e-147
Identity = 266/310 (85.81%), Postives = 273/310 (88.06%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKVSVVSRSGREVV GGIQLSDSASVADLQDAI+ RTKKFYPARQRLTLPVQPGSKERPT
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   REYCS+NSD+ITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG
Sbjct: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           Y+AERVIHPVQTYAMYYWCFHYFKRIMETFFV                        SYIA
Sbjct: 121 YQAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLY+PVGDLQMKIGFAFGLLCQV NFYCHILLRKLRSPEGNGGYQIPKGFLFNIV
Sbjct: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 279
           TCANYTTEIYQWVGFNIATQT AGY+FLVVA LIMTNWALAKHRRLKKLFDGKEGRP+YP
Sbjct: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. NCBI nr
Match: gi|255580033|ref|XP_002530850.1| (PREDICTED: very-long-chain enoyl-CoA reductase [Ricinus communis])

HSP 1 Score: 495.4 bits (1274), Expect = 6.7e-137
Identity = 239/310 (77.10%), Postives = 265/310 (85.48%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKV+VVSRSGREV+ GG+ L+DSA+VADLQ+AI+ RTKKFYP+RQRLTLP+ PGSK+RPT
Sbjct: 1   MKVTVVSRSGREVIKGGLDLNDSATVADLQEAIHKRTKKFYPSRQRLTLPLPPGSKDRPT 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   ++YC  NSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYP+FY+FPVY++FG
Sbjct: 61  VLNYKKSLKDYCDGNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPVFYFFPVYKYFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           YK ERVIHPVQTYAMYYWCFHYFKRIMETFFV                        SYIA
Sbjct: 121 YKEERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLY+PVGDLQMKIGF FGL+CQ+ NFYCHILL+ LRSP+G+GGYQIP GFLFNIV
Sbjct: 181 YYVNHPLYTPVGDLQMKIGFGFGLICQIANFYCHILLKNLRSPDGSGGYQIPHGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 279
           TCANYTTEIYQW+GFNIATQTVAGYIFLVVA  IMTNWALAKHRRL+KLFDGK+GRP+YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTVAGYIFLVVAASIMTNWALAKHRRLRKLFDGKDGRPKYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. NCBI nr
Match: gi|657981584|ref|XP_008382818.1| (PREDICTED: very-long-chain enoyl-CoA reductase [Malus domestica])

HSP 1 Score: 490.0 bits (1260), Expect = 2.8e-135
Identity = 237/310 (76.45%), Postives = 263/310 (84.84%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKV+VVSRSGREVV GG++LSDSA+VADLQDAI+ RTKKFYPARQRLTLPVQPGSKERP 
Sbjct: 1   MKVTVVSRSGREVVKGGLELSDSATVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPV 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   ++Y S NSDN+TVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVY + G
Sbjct: 61  VLSYKKSLQDYISGNSDNLTVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYDYLG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           +K +RVIHPVQTYA+YYWCFHYFKRIMETFFV                        ++IA
Sbjct: 121 FKGDRVIHPVQTYALYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGAFIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YY+NHPLY+PV DLQMKIGF  G++CQ+ NFYCHILLR LRSP+GNGGYQIP+GFLFNIV
Sbjct: 181 YYLNHPLYTPVSDLQMKIGFGIGIICQISNFYCHILLRNLRSPDGNGGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 279
           TCANYTTEIYQW+GFNIATQTVAGYIFL+VA  IMTNWALAKHRRLKK+FDGK+GRP+YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTVAGYIFLIVAASIMTNWALAKHRRLKKIFDGKDGRPKYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. NCBI nr
Match: gi|478435709|gb|AGJ00072.1| (enoyl-ACP reductase [Malus domestica])

HSP 1 Score: 489.2 bits (1258), Expect = 4.8e-135
Identity = 236/310 (76.13%), Postives = 262/310 (84.52%), Query Frame = 1


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKV+VVSRSGREVV GG++L+DSA+VADLQDAIY RTKKFYPARQRLTLPVQPGSKERP 
Sbjct: 1   MKVTVVSRSGREVVKGGLELTDSATVADLQDAIYKRTKKFYPARQRLTLPVQPGSKERPV 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   ++Y S NSDN+TVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVY + G
Sbjct: 61  VLSYKKSLQDYISGNSDNLTVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYDYLG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           +K +RVIHPVQTYA+YYWCFHYFKRIMETFFV                        ++IA
Sbjct: 121 FKGDRVIHPVQTYALYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGAFIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YY+NHPLY+PV DLQMKIGF FG++CQ+ N YCHILLR LRSP+GNGGYQIP+GFLFNIV
Sbjct: 181 YYLNHPLYTPVSDLQMKIGFGFGIICQISNLYCHILLRNLRSPDGNGGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 279
           TCANYTTEIYQW+GFNIATQTVAGYIFL+VA  IMTNWAL KHRRLKK+FDGK+GRP+YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTVAGYIFLIVAASIMTNWALTKHRRLKKIFDGKDGRPKYP 300

The following BLAST results are available for this feature:

BLAST of Cp4.1LG01g23900 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TECR_ARATH	8.2e-127	70.00	Very-long-chain enoyl-CoA reductase OS=Arabidopsis thaliana GN=ECR PE=1 SV=1	[more]
TECR_DICDI	6.7e-36	34.30	Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum GN=gpsn2 PE=3 SV...	[more]
TECR_CAEEL	1.8e-33	33.70	Probable very-long-chain enoyl-CoA reductase art-1 OS=Caenorhabditis elegans GN=...	[more]
TECR_HUMAN	7.1e-30	33.01	Very-long-chain enoyl-CoA reductase OS=Homo sapiens GN=TECR PE=1 SV=1	[more]
TECR_BOVIN	1.6e-29	33.01	Very-long-chain enoyl-CoA reductase OS=Bos taurus GN=TECR PE=2 SV=1	[more]

BLAST of Cp4.1LG01g23900 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KNT8_CUCSA	3.4e-148	86.13	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G517090 PE=4 SV=1	[more]
B9SXZ3_RICCO	4.7e-137	77.10	Synaptic glycoprotein SC2, putative OS=Ricinus communis GN=RCOM_1206840 PE=4 SV=...	[more]
A0A061FMS3_THECC	3.3e-135	76.13	3-oxo-5-alpha-steroid 4-dehydrogenase family protein isoform 1 OS=Theobroma caca...	[more]
M9T3Q3_MALDO	3.3e-135	76.13	Enoyl-ACP reductase OS=Malus domestica PE=2 SV=1	[more]
M9T617_MALDO	9.7e-135	75.81	Enoyl-ACP reductase OS=Malus domestica PE=2 SV=1	[more]

BLAST of Cp4.1LG01g23900 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G55360.1	4.6e-128	70.00	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT5G16010.1	1.6e-08	33.01	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

BLAST of Cp4.1LG01g23900 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449450010\|ref\|XP_004142757.1\|	4.9e-148	86.13	PREDICTED: very-long-chain enoyl-CoA reductase [Cucumis sativus]	[more]
gi\|659117972\|ref\|XP_008458883.1\|	1.4e-147	85.81	PREDICTED: very-long-chain enoyl-CoA reductase [Cucumis melo]	[more]
gi\|255580033\|ref\|XP_002530850.1\|	6.7e-137	77.10	PREDICTED: very-long-chain enoyl-CoA reductase [Ricinus communis]	[more]
gi\|657981584\|ref\|XP_008382818.1\|	2.8e-135	76.45	PREDICTED: very-long-chain enoyl-CoA reductase [Malus domestica]	[more]
gi\|478435709\|gb\|AGJ00072.1\|	4.8e-135	76.13	enoyl-ACP reductase [Malus domestica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane
GO:0005737	cytoplasm

Vocabulary: **Biological Process**
Term	Definition
GO:0006629	lipid metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors

Vocabulary: **INTERPRO**
Term	Definition
IPR001104	3-oxo-5_a-steroid_4-DH_C

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042967	acyl-carrier-protein biosynthetic process
biological_process	GO:0009651	response to salt stress
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0008209	androgen metabolic process
biological_process	GO:0016126	sterol biosynthetic process
biological_process	GO:0046520	sphingoid biosynthetic process
biological_process	GO:0010025	wax biosynthetic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0007030	Golgi organization
biological_process	GO:0006633	fatty acid biosynthetic process
biological_process	GO:0008210	estrogen metabolic process
biological_process	GO:0006816	calcium ion transport
biological_process	GO:0006699	bile acid biosynthetic process
cellular_component	GO:0009923	fatty acid elongase complex
cellular_component	GO:0005835	fatty acid synthase complex
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0003865	3-oxo-5-alpha-steroid 4-dehydrogenase activity
molecular_function	GO:0004318	enoyl-[acyl-carrier-protein] reductase (NADH) activity
molecular_function	GO:0009922	fatty acid elongase activity
molecular_function	GO:0019166	trans-2-enoyl-CoA reductase (NADPH) activity
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG01g23900.1	Cp4.1LG01g23900.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 132..277 score: 4.6
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROFILE	PS50244	S5A_REDUCTASE	coord: 167..248 score: 21
None	No IPR available	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 2..278 score: 2.9E
None	No IPR available	PANTHER	PTHR10556:SF28	SC2	coord: 2..278 score: 2.9E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG01g23900	Cucsa.010800	Cucumber (Gy14) v1	cgycpeB0522
Cp4.1LG01g23900	CmaCh15G003870	Cucurbita maxima (Rimu)	cmacpeB317
Cp4.1LG01g23900	CmoCh15G003980	Cucurbita moschata (Rifu)	cmocpeB279
Cp4.1LG01g23900	Cla004664	Watermelon (97103) v1	cpewmB381
Cp4.1LG01g23900	Csa5G517090	Cucumber (Chinese Long) v2	cpecuB417
Cp4.1LG01g23900	MELO3C021634	Melon (DHL92) v3.5.1	cpemeB358
Cp4.1LG01g23900	ClCG09G019780	Watermelon (Charleston Gray)	cpewcgB344
Cp4.1LG01g23900	CSPI05G17720	Wild cucumber (PI 183967)	cpecpiB416
Cp4.1LG01g23900	Lsi01G014210	Bottle gourd (USVL1VR-Ls)	cpelsiB324
Cp4.1LG01g23900	Lsi02G016350	Bottle gourd (USVL1VR-Ls)	cpelsiB336
Cp4.1LG01g23900	MELO3C021634.2	Melon (DHL92) v3.6.1	cpemedB418
Cp4.1LG01g23900	CsaV3_5G026640	Cucumber (Chinese Long) v3	cpecucB0519
Cp4.1LG01g23900	Bhi06G000532	Wax gourd	cpewgoB0502
Cp4.1LG01g23900	CsGy5G017380	Cucumber (Gy14) v2	cgybcpeB627
Cp4.1LG01g23900	Carg04501	Silver-seed gourd	carcpeB0965
Cp4.1LG01g23900	Carg02349	Silver-seed gourd	carcpeB0374

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Cp4.1LG01g23900	Cp4.1LG13g09400	Cucurbita pepo (Zucchini)	cpecpeB199

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG01g23900	Cucurbita pepo (Zucchini)	cpecpeB384
Cp4.1LG01g23900	Cucurbita maxima (Rimu)	cmacpeB721
Cp4.1LG01g23900	Cucurbita moschata (Rifu)	cmocpeB673
Cp4.1LG01g23900	Cucumber (Chinese Long) v3	cpecucB0502
Cp4.1LG01g23900	Wax gourd	cpewgoB0541