CsaV3_5G025580 (gene) Cucumber (Chinese Long) v3 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsaV3_5G025580
Type	gene
Organism	Cucumis sativus (Cucumber (Chinese Long) v3)
Description	Cysteine proteinase inhibitor
Location	chr5 : 20689508 .. 20689969 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAAAATATATAAATCTATCTATCTATATCAGAAGGGTACGTACGTATTAGGAATGAGTTCAGAGGATATTTCAATTCCCAAGGTTGATCCAGCAGTGCCAATTAAGGATGTTGATAGCGAAGAGATACGAGGATTTGGAGAGTTTGCAGTGAGTGAGCATAATGAAGCAACGGGAGAAAATCTGGAGTTTGTGAAGGTGATAAATGGGTTGGAACAGGGACGAAGGGGTTTCCGAATAGACTTCACCCTTGTCCTGTCAGCTAAAAACTCTATTGGAATTATTTGGACTTATAAAGTTAAAGTCTTTCTTAATCAACGGGCTGGAGTACGTCTCTTGGAGTTTGAAGCTGTGCTTAATGAGAATGGAAACCAAAGCTGAATGTGATATGTCTACTCAACAATATAATTGTTGTATGCAAACATATATATGAAATAAAATAAAATAAATGATTATTATGATA

mRNA sequence

AAAATATATAAATCTATCTATCTATATCAGAAGGGTACGTACGTATTAGGAATGAGTTCAGAGGATATTTCAATTCCCAAGGTTGATCCAGCAGTGCCAATTAAGGATGTTGATAGCGAAGAGATACGAGGATTTGGAGAGTTTGCAGTGAGTGAGCATAATGAAGCAACGGGAGAAAATCTGGAGTTTGTGAAGGTGATAAATGGGTTGGAACAGGGACGAAGGGGTTTCCGAATAGACTTCACCCTTGTCCTGTCAGCTAAAAACTCTATTGGAATTATTTGGACTTATAAAGTTAAAGTCTTTCTTAATCAACGGGCTGGAGTACGTCTCTTGGAGTTTGAAGCTGTGCTTAATGAGAATGGAAACCAAAGCTGA

Coding sequence (CDS)

AAAATATATAAATCTATCTATCTATATCAGAAGGGTACGTACGTATTAGGAATGAGTTCAGAGGATATTTCAATTCCCAAGGTTGATCCAGCAGTGCCAATTAAGGATGTTGATAGCGAAGAGATACGAGGATTTGGAGAGTTTGCAGTGAGTGAGCATAATGAAGCAACGGGAGAAAATCTGGAGTTTGTGAAGGTGATAAATGGGTTGGAACAGGGACGAAGGGGTTTCCGAATAGACTTCACCCTTGTCCTGTCAGCTAAAAACTCTATTGGAATTATTTGGACTTATAAAGTTAAAGTCTTTCTTAATCAACGGGCTGGAGTACGTCTCTTGGAGTTTGAAGCTGTGCTTAATGAGAATGGAAACCAAAGCTGA

Protein sequence

KIYKSIYLYQKGTYVLGMSSEDISIPKVDPAVPIKDVDSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGRRGFRIDFTLVLSAKNSIGIIWTYKVKVFLNQRAGVRLLEFEAVLNENGNQS

Homology

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BLAST of CsaV3_5G025580 vs. NCBI nr
Match: KGN51192.1 (hypothetical protein Csa_5G487690 [Cucumis sativus])

HSP 1 Score: 214.2 bits (544), Expect = 2.6e-52
Identity = 108/108 (100.00%), Postives = 108/108 (100.00%), Query Frame = 0


Query: 18  MSSEDISIPKVDPAVPIKDVDSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGRRGF 77
           MSSEDISIPKVDPAVPIKDVDSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGRRGF
Sbjct: 1   MSSEDISIPKVDPAVPIKDVDSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGRRGF 60

Query: 78  RIDFTLVLSAKNSIGIIWTYKVKVFLNQRAGVRLLEFEAVLNENGNQS 126
           RIDFTLVLSAKNSIGIIWTYKVKVFLNQRAGVRLLEFEAVLNENGNQS
Sbjct: 61  RIDFTLVLSAKNSIGIIWTYKVKVFLNQRAGVRLLEFEAVLNENGNQS 108

[Close]

BLAST of CsaV3_5G025580 vs. NCBI nr
Match: KGN51193.1 (hypothetical protein Csa_5G487700 [Cucumis sativus])

HSP 1 Score: 138.7 bits (348), Expect = 1.4e-29
Identity = 73/99 (73.74%), Postives = 81/99 (81.82%), Query Frame = 0


Query: 25  IPKVDPAVPIKDVDSEEIRGFGEFAVSEHNE-ATGENLEFVKVINGLEQGRRGFRIDFTL 84
           +P  D  VPIKDVDSEEI+G G  AV  HNE +TGENLEFVKVINGLE+GRRGF+I +TL
Sbjct: 15  LPDYD-TVPIKDVDSEEIQGIGGMAVRMHNETSTGENLEFVKVINGLEKGRRGFQIVYTL 74

Query: 85  VLSAKNSIGIIWTYKVKVFLNQRAGVRLLEFEAVLNENG 123
           VLSAKNS+GI+WTYK KV  N RA VRL EFEAVL ENG
Sbjct: 75  VLSAKNSLGIVWTYKAKVIFNNRAAVRLPEFEAVLPENG 112

[Close]

BLAST of CsaV3_5G025580 vs. NCBI nr
Match: KGN51190.1 (Cystatin [Cucumis sativus])

HSP 1 Score: 64.3 bits (155), Expect = 3.4e-07
Identity = 28/45 (62.22%), Postives = 34/45 (75.56%), Query Frame = 0


Query: 30 PAVPIKDVDSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGR 75
          P VPIKD+DS EI+G+  FA   HNE TG+ L+F + INGLEQGR
Sbjct: 9  PPVPIKDIDSAEIQGYARFATERHNEITGDKLQFQRAINGLEQGR 53

[Close]

BLAST of CsaV3_5G025580 vs. NCBI nr
Match: XP_024314832.1 (uncharacterized protein LOC100828052 [Brachypodium distachyon])

HSP 1 Score: 53.9 bits (128), Expect = 4.5e-04
Identity = 31/82 (37.80%), Postives = 47/82 (57.32%), Query Frame = 0


Query: 33  PIKDVDSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGRRGFRIDFTLVLSAKNSIG 92
           PI D+D  EI+  G++AV+EH +   + L+F KV+ G  Q   GF  ++ L + AKNS G
Sbjct: 266 PIPDIDKPEIQELGQWAVAEHVKQAKDGLKFTKVVKGEVQVVAGF--NYRLDIHAKNSDG 325

Query: 93  IIWTYKVKVFLNQRAGVRLLEF 115
           +   Y+  V+     G RL+ F
Sbjct: 326 VDGAYRTVVWELPTNGRRLISF 345

[Close]

BLAST of CsaV3_5G025580 vs. NCBI nr
Match: KQK22987.1 (hypothetical protein BRADI_1g70500v3 [Brachypodium distachyon])

HSP 1 Score: 53.9 bits (128), Expect = 4.5e-04
Identity = 31/82 (37.80%), Postives = 47/82 (57.32%), Query Frame = 0


Query: 33  PIKDVDSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGRRGFRIDFTLVLSAKNSIG 92
           PI D+D  EI+  G++AV+EH +   + L+F KV+ G  Q   GF  ++ L + AKNS G
Sbjct: 32  PIPDIDKPEIQELGQWAVAEHVKQAKDGLKFTKVVKGEVQVVAGF--NYRLDIHAKNSDG 91

Query: 93  IIWTYKVKVFLNQRAGVRLLEF 115
           +   Y+  V+     G RL+ F
Sbjct: 92  VDGAYRTVVWELPTNGRRLISF 111

[Close]

BLAST of CsaV3_5G025580 vs. TrEMBL
Match: tr|A0A0A0KRZ8|A0A0A0KRZ8_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_5G487690 PE=3 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 1.7e-52
Identity = 108/108 (100.00%), Postives = 108/108 (100.00%), Query Frame = 0


Query: 18  MSSEDISIPKVDPAVPIKDVDSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGRRGF 77
           MSSEDISIPKVDPAVPIKDVDSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGRRGF
Sbjct: 1   MSSEDISIPKVDPAVPIKDVDSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGRRGF 60

Query: 78  RIDFTLVLSAKNSIGIIWTYKVKVFLNQRAGVRLLEFEAVLNENGNQS 126
           RIDFTLVLSAKNSIGIIWTYKVKVFLNQRAGVRLLEFEAVLNENGNQS
Sbjct: 61  RIDFTLVLSAKNSIGIIWTYKVKVFLNQRAGVRLLEFEAVLNENGNQS 108

[Close]

BLAST of CsaV3_5G025580 vs. TrEMBL
Match: tr|A0A0A0KNL2|A0A0A0KNL2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G487700 PE=4 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 9.3e-30
Identity = 73/99 (73.74%), Postives = 81/99 (81.82%), Query Frame = 0


Query: 25  IPKVDPAVPIKDVDSEEIRGFGEFAVSEHNE-ATGENLEFVKVINGLEQGRRGFRIDFTL 84
           +P  D  VPIKDVDSEEI+G G  AV  HNE +TGENLEFVKVINGLE+GRRGF+I +TL
Sbjct: 15  LPDYD-TVPIKDVDSEEIQGIGGMAVRMHNETSTGENLEFVKVINGLEKGRRGFQIVYTL 74

Query: 85  VLSAKNSIGIIWTYKVKVFLNQRAGVRLLEFEAVLNENG 123
           VLSAKNS+GI+WTYK KV  N RA VRL EFEAVL ENG
Sbjct: 75  VLSAKNSLGIVWTYKAKVIFNNRAAVRLPEFEAVLPENG 112

[Close]

BLAST of CsaV3_5G025580 vs. TrEMBL
Match: tr|A0A0A0KNH4|A0A0A0KNH4_CUCSA (Cystatin OS=Cucumis sativus OX=3659 GN=Csa_5G485690 PE=4 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.2e-07
Identity = 28/45 (62.22%), Postives = 34/45 (75.56%), Query Frame = 0


Query: 30 PAVPIKDVDSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGR 75
          P VPIKD+DS EI+G+  FA   HNE TG+ L+F + INGLEQGR
Sbjct: 9  PPVPIKDIDSAEIQGYARFATERHNEITGDKLQFQRAINGLEQGR 53

[Close]

BLAST of CsaV3_5G025580 vs. TrEMBL
Match: tr|I1H8A5|I1H8A5_BRADI (Cysteine proteinase inhibitor OS=Brachypodium distachyon OX=15368 GN=LOC100828355 PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 3.0e-04
Identity = 31/82 (37.80%), Postives = 47/82 (57.32%), Query Frame = 0


Query: 33  PIKDVDSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGRRGFRIDFTLVLSAKNSIG 92
           PI D+D  EI+  G++AV+EH +   + L+F KV+ G  Q   GF  ++ L + AKNS G
Sbjct: 32  PIPDIDKPEIQELGQWAVAEHVKQAKDGLKFTKVVKGEVQVVAGF--NYRLDIHAKNSDG 91

Query: 93  IIWTYKVKVFLNQRAGVRLLEF 115
           +   Y+  V+     G RL+ F
Sbjct: 92  VDGAYRTVVWELPTNGRRLISF 111

[Close]

BLAST of CsaV3_5G025580 vs. TrEMBL
Match: tr|A0A1S3B5B9|A0A1S3B5B9_CUCME (Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103486185 PE=3 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 5.1e-04
Identity = 30/75 (40.00%), Postives = 44/75 (58.67%), Query Frame = 0


Query: 33  PIKDV-DSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGRRG-----FRIDFTLVLS 92
           PI+D+ D+ E++  G   V +HN+ TGENL+FV+V+NGL             + + LVL 
Sbjct: 9   PIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGGVLYELVLE 68

Query: 93  AKNSIGIIWTYKVKV 102
           A  +  I WTYK +V
Sbjct: 69  AITTSKINWTYKARV 83

The following BLAST results are available for this feature:

BLAST of CsaV3_5G025580 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN51192.1	2.6e-52	100.00	hypothetical protein Csa_5G487690 [Cucumis sativus]	[more]
KGN51193.1	1.4e-29	73.74	hypothetical protein Csa_5G487700 [Cucumis sativus]	[more]
KGN51190.1	3.4e-07	62.22	Cystatin [Cucumis sativus]	[more]
XP_024314832.1	4.5e-04	37.80	uncharacterized protein LOC100828052 [Brachypodium distachyon]	[more]
KQK22987.1	4.5e-04	37.80	hypothetical protein BRADI_1g70500v3 [Brachypodium distachyon]	[more]

BLAST of CsaV3_5G025580 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of CsaV3_5G025580 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of CsaV3_5G025580 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KRZ8\|A0A0A0KRZ8_CUCSA	1.7e-52	100.00	Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_5G487690 PE=3 SV...	[more]
tr\|A0A0A0KNL2\|A0A0A0KNL2_CUCSA	9.3e-30	73.74	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G487700 PE=4 SV=1	[more]
tr\|A0A0A0KNH4\|A0A0A0KNH4_CUCSA	2.2e-07	62.22	Cystatin OS=Cucumis sativus OX=3659 GN=Csa_5G485690 PE=4 SV=1	[more]
tr\|I1H8A5\|I1H8A5_BRADI	3.0e-04	37.80	Cysteine proteinase inhibitor OS=Brachypodium distachyon OX=15368 GN=LOC10082835...	[more]
tr\|A0A1S3B5B9\|A0A1S3B5B9_CUCME	5.1e-04	40.00	Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103486185 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004869	cysteine-type endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR027214	Cystatin
IPR000010	Cystatin_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_5G025580.1	CsaV3_5G025580.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 34..113 e-value: 6.9E-14 score: 51.9
None	No IPR available	GENE3D	G3DSA:3.10.450.10		coord: 30..123 e-value: 9.2E-13 score: 50.0
None	No IPR available	PANTHER	PTHR11413:SF65	CYSTEINE PROTEINASE INHIBITOR 5	coord: 32..110
None	No IPR available	PRODOM	PD001231		coord: 42..102 e-value: 3.0E-5 score: 107.0
None	No IPR available	SUPERFAMILY	SSF54403	Cystatin/monellin	coord: 26..105
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 32..110

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsaV3_5G025580	CsGy5G016360	Cucumber (Gy14) v2	cgybcucB232
CsaV3_5G025580	Cla003258	Watermelon (97103) v1	cucwmB387
CsaV3_5G025580	ClCG09G020790	Watermelon (Charleston Gray)	cucwcgB362
CsaV3_5G025580	Lsi02G017400	Bottle gourd (USVL1VR-Ls)	cuclsiB370
CsaV3_5G025580	Cla97C09G182210	Watermelon (97103) v2	cucwmbB424
CsaV3_5G025580	Bhi06G000662	Wax gourd	cucwgoB484

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsaV3_5G025580	Silver-seed gourd	carcucB1110
CsaV3_5G025580	Cucumber (Gy14) v1	cgycucB422
CsaV3_5G025580	Cucurbita maxima (Rimu)	cmacucB0892
CsaV3_5G025580	Cucurbita maxima (Rimu)	cmacucB0947
CsaV3_5G025580	Cucurbita moschata (Rifu)	cmocucB0205
CsaV3_5G025580	Cucurbita moschata (Rifu)	cmocucB0935
CsaV3_5G025580	Cucurbita pepo (Zucchini)	cpecucB0129
CsaV3_5G025580	Cucurbita pepo (Zucchini)	cpecucB0550
CsaV3_5G025580	Cucurbita pepo (Zucchini)	cpecucB1028
CsaV3_5G025580	Wild cucumber (PI 183967)	cpicucB284
CsaV3_5G025580	Melon (DHL92) v3.5.1	cucmeB359
CsaV3_5G025580	Melon (DHL92) v3.6.1	cucmedB351