CsaV3_4G007470 (gene) Cucumber (Chinese Long) v3 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsaV3_4G007470
Type	gene
Organism	Cucumis sativus (Cucumber (Chinese Long) v3)
Description	DNA topoisomerase
Location	chr4 : 5091483 .. 5095042 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTAATTTTTATTTTGCATTTTTTAAACACGTGGCAAGCAGGTAGTTCTAATTTTATATCACAAAACCCTAACGAGGATGTTACTACACCAAAGGCCGGAAACGAAACCCTGACGCAAAATTTGAAACCTATTCCGATCAAGTGGACGAAGTTGGAATCCAAGACCAATTTTGATTTCCATCTCCCAGAAGAAGACGATGAAGAGTTCCTCTTTGCAGTGGCGGACAGCAGCTGAAGTTGTTTCCCTAGTCAAGAAAGTCAGAAATGTCCCTAACCCTACCATCGATCTCCCCAAAAATGCCGTTGCGGAGGTTTCGCATAAAGTTCCCCTTAATGCAGACTTTTTAGGGACCTACATCGCCCCTTATAAAGGAAGCCGTGCTCTCTCTCAAATTTCAGGCTCGGAAGCCAGTTCTGACAAAAATGTATCCATTTATGAAAATACTACTGCTCAATCGGAAGGCGATGGTTCGTGTTTCAAATGCGGGAAATTAGGGCATTGGGCTAGAGACTGCGATGCTCCGGGTGGTGGAGGATCTTTCAGTTCTTCAGGAAATGATATGTCTGTTCCGGATAAAGCATGCCCCTGCGGATCGGGAATTTGTTCAGTATTAACTGCGAATACGGAGAGGAATCGCGGTCGAAAATTTTACAAATGCCCCGTTCGGCAGGTGATGAAGGAAATGTGTAGTAGTATCATTTCTTCTTCTTTCTTTTATCGCCATTTTTTAAGTCATAATGTTGCAGCGTAAACTTGGGAAATGGAAGAGAGATTTCGTGTTCTGATGAAGTCATGTGCTTTTTCATCTAGTTGATGTAGGGTTTTGTGAAACATATATAAACTTCTATTAAGAACCTCGAGAAAGATATTGATGTTACTTAAACCTTTGTTATAATCATCTAGAAATTAATTTAGTAATGTGTAATGGATTCATATAATTTTTGTTGATTAAAACGTACTTGTTCTTTAACCAACTGGGTCTAATAGATTAGGGTACTAGTACCCTTTTCAAAAAATTGTGGAAGTACTTTTTTAAAGTCTTAAGTTTGTTATTATCATTTTGAATTCAAAGCTCATTAAACTGTTCCTGACGCAATAACTTTTATAATTACATGTTAGTATACAATGCCCTTACTTTCTTCTCATGTTCATACAATTGTTCTCAGGAAAATGGTGGCTGCGGATTCTTTGAGTGGTGTGACAGTGCCTCTGTAGCTAATGTAGTGTCTTATGGAAGCCAAAATCCATTAAGTTCGTCATTTTCAGACCTTCAGTGCCCCTGTGGTGCTGGTTCTTGCAAAATTCTAACAGCTAAAACAGGGAACAACGTTGGGAAGCAGTTCTATTGTTGCCCTTCATCCCAGGTAAGTTTTATAACACAAATGTTCGTGCAGCAGCTTTTATTGTCTTGTGATGACGTGATTCTCAAAACTGTTTGTTCATAATTAAGAGTCATAGCTTCTCTACCTTATTGAAGTGGAAACATCTTTTGTATTCCCTTTAGTTGAGACTCTTTTGTATATCATTGGATATTTCATCAATCTATGAAATAGTTTCTTATCTAAGAAGAAGATTTCGGTTTTGAATTTTCATTTTCATATTAAGAAGTCATGGACTATCCAATTATCTGACTGACAACTGTGGGAAAGCTTCATTCTGATTGATTGGGAGAAAGTAAAATTTGCTCTGGTACCTTGTCTAAATTTATTGCTTCTCATTTATTCTTCTCATTCTTCCAAATTTAATTTAGAGTCCCAATTTTATGACTTTTCATCTTTAGATCAATTGATGGTGCTGGAAGTTGAAAATTATGACCTTGAAGGTGGCTGAAATAATTGCAAACAATTGGATAGTCGCCAATTACTCTGCATACTTATTAATTGAATGAAGCGGATAAAGAGGGATGTTATAGTTGCATTATGAACTAAGGAAAGAAAGCAAGATCCAATGCATGTGCGAGCGGTATATAATAAGAATGTGCTTAGTGAAGTTTTGGAGAGGACGTTTATCAAGGAAAACACAGCTCTAGATCTTTTAAGCCAAGGTATCTAAACAAATTTTCTGAGATGACTTTTATCTAGGAAAACACGTGTAACCCGATTTAGAGCACAAACAAGAGCAAATTAATTTAATTCTAACTCTACGCACAAATTAATTGAAGAGAATGCAAGTTCTTTGATCGGAATACCTACTATAGGAGAGGTAAAAAGGGGAATAATCACCAGAAAATAGGTAAGTACACCTGTTGGGCATGAGTGTGTAAGTAGGCCTTTAAGGATCAGGTTGTGTTGCGGGCAGGGGTATGGTATTTTTGTAGTTGAACACTGTTCTAAGGTTTAGGATAGTTTCTTTAATTTGATGGGTTTTCATTTTTGTTTGTTTTTCAATGTTGCTATTGTTGCCTCAACTTACATTTCAATGAAATAGTGAGGGAATACCCTTGCAGAAAATTAGTGGATTGTCGTTCTGTAGTTTTTCTCCCAGCCTTTTAGGTGGTTGGGGTGCTTGATTGATTAGTCCTTGATATGAGAAATTTCATCTTCAATTATGCATAGCACTATGATATGAAACCCTTCCAAAGTTTATCCTTCTATGTGTCCACCATGATCTATCCAGTCCCAACATCTCTTCAAATTTACACCTTGTGTGGTAATGGCAATTTCCGTCACCTCCCACATCTGGAGGAGTCTCAACATCAACCAAACACAATTTTCTGAAAATGCTTTGATACTTACAAATTGCACAAGTTATGCAGTGGATTGATTTCCATGTTGTAATTTTTCATCAGTTGAACTCATCTTTTCTGCAGGCTTCTTGCAGGTTTTTCCGATGGTGCGAAGAGCCTTCCATGGAAACCAAGAATCAAGAAAGCACCCCTAAGGTCTACGGTAACACAAATGACACAAGCAAAAGTGCTACCTTGAATGTGAAGACTAGTTCTTCCAGTTCCAGCTATAAATATGGGAGTGAAGGGCATTGGGCTAGAGACTGGTCGCAATCACCCTCATCTTCAACTTCTCCTGCAGAGTATGGCAGAAGTCAATCTTCTTCAGCTGGAAGCTGTTTCAAATGTGGTATGCCTGGCCATTGGGCGAGGGACTGCTCTAATTTTGAAAAATAGGTACCGACTCTTTGGCTGCTTCGGCATTAAGCTGTTTCAAGGTGTTTAGCCGTTGTTTAAGCTTTTAAATCCATAAGTGCTGAATATGTCATCTGGCATTTCTGTTTATTTGGTACACTCGCCTATTTTGAAGTACACTTTTGGGGATGATGTCTAGATGGATTAGCTTAGAATTGAGACTTTAGTATTGAGTCTCTTAAATTTGAATTTTTATCATTTAATCATCGATTATTTACTTATCTAAATATTATTAAAAGTAAGTTTAATTTAATTCTTTTTTTCTCATCTGTAGTATATATAAAAGAATGGAAGGAGTATATAAAAGGATGGGAGTGGAGTAATTTTTTAGTCTCATTATATCATTTCATTATAACATTTTTTAGATTTGGTTTAGTGGTAATTTTGTCATTTAGATATATTTAATATTTAATGTTTGTG

mRNA sequence

ATGAAGAGTTCCTCTTTGCAGTGGCGGACAGCAGCTGAAGTTGTTTCCCTAGTCAAGAAAGTCAGAAATGTCCCTAACCCTACCATCGATCTCCCCAAAAATGCCGTTGCGGAGGTTTCGCATAAAGTTCCCCTTAATGCAGACTTTTTAGGGACCTACATCGCCCCTTATAAAGGAAGCCGTGCTCTCTCTCAAATTTCAGGCTCGGAAGCCAGTTCTGACAAAAATGTATCCATTTATGAAAATACTACTGCTCAATCGGAAGGCGATGGTTCGTGTTTCAAATGCGGGAAATTAGGGCATTGGGCTAGAGACTGCGATGCTCCGGGTGGTGGAGGATCTTTCAGTTCTTCAGGAAATGATATGTCTGTTCCGGATAAAGCATGCCCCTGCGGATCGGGAATTTGTTCAGTATTAACTGCGAATACGGAGAGGAATCGCGGTCGAAAATTTTACAAATGCCCCGTTCGGCAGGAAAATGGTGGCTGCGGATTCTTTGAGTGGTGTGACAGTGCCTCTGTAGCTAATGTAGTGTCTTATGGAAGCCAAAATCCATTAAGTTCGTCATTTTCAGACCTTCAGTGCCCCTGTGGTGCTGGTTCTTGCAAAATTCTAACAGCTAAAACAGGGAACAACGTTGGGAAGCAGTTCTATTGTTGCCCTTCATCCCAGGTAAGTTTTATAACACAAATGTTCGTGCAGCAGCTTTTATTGTCTTGTGATGACGTGATTCTCAAAACTGTTTGTTCATAA

Coding sequence (CDS)

ATGAAGAGTTCCTCTTTGCAGTGGCGGACAGCAGCTGAAGTTGTTTCCCTAGTCAAGAAAGTCAGAAATGTCCCTAACCCTACCATCGATCTCCCCAAAAATGCCGTTGCGGAGGTTTCGCATAAAGTTCCCCTTAATGCAGACTTTTTAGGGACCTACATCGCCCCTTATAAAGGAAGCCGTGCTCTCTCTCAAATTTCAGGCTCGGAAGCCAGTTCTGACAAAAATGTATCCATTTATGAAAATACTACTGCTCAATCGGAAGGCGATGGTTCGTGTTTCAAATGCGGGAAATTAGGGCATTGGGCTAGAGACTGCGATGCTCCGGGTGGTGGAGGATCTTTCAGTTCTTCAGGAAATGATATGTCTGTTCCGGATAAAGCATGCCCCTGCGGATCGGGAATTTGTTCAGTATTAACTGCGAATACGGAGAGGAATCGCGGTCGAAAATTTTACAAATGCCCCGTTCGGCAGGAAAATGGTGGCTGCGGATTCTTTGAGTGGTGTGACAGTGCCTCTGTAGCTAATGTAGTGTCTTATGGAAGCCAAAATCCATTAAGTTCGTCATTTTCAGACCTTCAGTGCCCCTGTGGTGCTGGTTCTTGCAAAATTCTAACAGCTAAAACAGGGAACAACGTTGGGAAGCAGTTCTATTGTTGCCCTTCATCCCAGGTAAGTTTTATAACACAAATGTTCGTGCAGCAGCTTTTATTGTCTTGTGATGACGTGATTCTCAAAACTGTTTGTTCATAA

Protein sequence

MKSSSLQWRTAAEVVSLVKKVRNVPNPTIDLPKNAVAEVSHKVPLNADFLGTYIAPYKGSRALSQISGSEASSDKNVSIYENTTAQSEGDGSCFKCGKLGHWARDCDAPGGGGSFSSSGNDMSVPDKACPCGSGICSVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDSASVANVVSYGSQNPLSSSFSDLQCPCGAGSCKILTAKTGNNVGKQFYCCPSSQVSFITQMFVQQLLLSCDDVILKTVCS

Homology

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BLAST of CsaV3_4G007470 vs. NCBI nr
Match: XP_004147743.1 (PREDICTED: DNA topoisomerase 3-alpha-like [Cucumis sativus] >KGN53505.1 hypothetical protein Csa_4G062410 [Cucumis sativus])

HSP 1 Score: 465.3 bits (1196), Expect = 1.3e-127
Identity = 225/226 (99.56%), Postives = 225/226 (99.56%), Query Frame = 0


Query: 1   MKSSSLQWRTAAEVVSLVKKVRNVPNPTIDLPKNAVAEVSHKVPLNADFLGTYIAPYKGS 60
           MKSSSLQWRTAAEVVSLVKKVRNVPNPTIDLPKNAVAEVSHKVPLNADFLGTYIAPYKGS
Sbjct: 1   MKSSSLQWRTAAEVVSLVKKVRNVPNPTIDLPKNAVAEVSHKVPLNADFLGTYIAPYKGS 60

Query: 61  RALSQISGSEASSDKNVSIYENTTAQSEGDGSCFKCGKLGHWARDCDAPGGGGSFSSSGN 120
           RALSQISGSEASSDKNVSIYENTTAQSEGDGSCFKCGKLGHWARDCDAPGGGGSFSSSGN
Sbjct: 61  RALSQISGSEASSDKNVSIYENTTAQSEGDGSCFKCGKLGHWARDCDAPGGGGSFSSSGN 120

Query: 121 DMSVPDKACPCGSGICSVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDSASVANVVSY 180
           DMSVPDKACPCGSGICSVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDSASVANVVSY
Sbjct: 121 DMSVPDKACPCGSGICSVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDSASVANVVSY 180

Query: 181 GSQNPLSSSFSDLQCPCGAGSCKILTAKTGNNVGKQFYCCPSSQVS 227
           GSQNPLSSSFSDLQCPCGAGSCKILTAKTGNNVGKQFYCCPSSQ S
Sbjct: 181 GSQNPLSSSFSDLQCPCGAGSCKILTAKTGNNVGKQFYCCPSSQAS 226

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BLAST of CsaV3_4G007470 vs. NCBI nr
Match: XP_022931285.1 (cold shock domain-containing protein 3-like [Cucurbita moschata])

HSP 1 Score: 302.0 bits (772), Expect = 1.9e-78
Identity = 154/220 (70.00%), Postives = 170/220 (77.27%), Query Frame = 0


Query: 11  AAEVVSLVKKVRNVPNPTIDLPKNAVAEVSHKVPLNADFLGTYIAPYKGSR-ALSQISGS 70
           AAE  +L  K R +PN TID PK A+AEV+ KV  NAD  G YIA  KG    LSQ+SG 
Sbjct: 28  AAEARALASKRRKIPNHTIDPPKRAIAEVTSKVSPNADAEGIYIAALKGDPIILSQLSGR 87

Query: 71  EASSDKNVSIYENTT--AQSEGDGSCFKCGKLGHWARDCDAPGGGGSFSSSGNDMSVPDK 130
            A+S     I  NTT  AQSEG GSCFKCGKLGHWARDCDAPG GGSFS SGND SV +K
Sbjct: 88  IANSGNKGFISANTTAHAQSEGGGSCFKCGKLGHWARDCDAPGSGGSFSFSGNDTSVAEK 147

Query: 131 ACPCGSGICSVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDSASVANVVSYGSQN-PL 190
           +CPCG G+C VLTANTERNRGRKFYKCPVRQENGGCGFFEWCD+AS  N+++YGSQN   
Sbjct: 148 SCPCGMGVCVVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDNASATNIMTYGSQNRAS 207

Query: 191 SSSFSDLQCPCGAGSCKILTAKTGNNVGKQFYCCPSSQVS 227
           SSSFSDL+CPCGAGSC  LTAKTG NVG+QFY CPS Q +
Sbjct: 208 SSSFSDLKCPCGAGSCITLTAKTGENVGQQFYRCPSYQAT 247

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BLAST of CsaV3_4G007470 vs. NCBI nr
Match: XP_023552737.1 (cold shock domain-containing protein 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 300.1 bits (767), Expect = 7.3e-78
Identity = 154/220 (70.00%), Postives = 171/220 (77.73%), Query Frame = 0


Query: 11  AAEVVSLVKKVRNVPNPTIDLPKNAVAEVSHKVPLNADFLGTYIAPYKGSR-ALSQISGS 70
           AAE  +L  K R +PN TID PK AVAEV+ KV  NAD  G YIA  KG    LSQ+SG 
Sbjct: 27  AAEARALASKRRKIPNHTIDPPKRAVAEVTSKVSPNADAEGIYIAALKGDPIILSQLSGR 86

Query: 71  EASSDKNVSIYENTT--AQSEGDGSCFKCGKLGHWARDCDAPGGGGSFSSSGNDMSVPDK 130
            A+S     I  NTT  AQSEG GSCFKCGKLGHWARDCDAPG GGSFS+SGND SV +K
Sbjct: 87  IANSGNKGFISANTTAHAQSEGGGSCFKCGKLGHWARDCDAPGSGGSFSASGNDTSVAEK 146

Query: 131 ACPCGSGICSVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDSASVANVVSYGSQN-PL 190
           +CPCG G+C VLTANTERNRGRKFYKC VRQENGGCGFFEWCD+AS  N+++YGSQN   
Sbjct: 147 SCPCGMGVCVVLTANTERNRGRKFYKCAVRQENGGCGFFEWCDNASSTNIMTYGSQNRAS 206

Query: 191 SSSFSDLQCPCGAGSCKILTAKTGNNVGKQFYCCPSSQVS 227
           SSSFSDL+CPCGAGSC  LTAKTG+NVG+QFY CPS Q +
Sbjct: 207 SSSFSDLKCPCGAGSCITLTAKTGDNVGQQFYRCPSYQAT 246

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BLAST of CsaV3_4G007470 vs. NCBI nr
Match: XP_022984959.1 (cold shock domain-containing protein 3-like [Cucurbita maxima])

HSP 1 Score: 296.2 bits (757), Expect = 1.0e-76
Identity = 150/220 (68.18%), Postives = 170/220 (77.27%), Query Frame = 0


Query: 11  AAEVVSLVKKVRNVPNPTIDLPKNAVAEVSHKVPLNADFLGTYIAPYKGSR-ALSQISGS 70
           AAE  +L  K R +PN TI+ PK A+AEV++KV  N D  G YIA  KG    LSQ+SG 
Sbjct: 28  AAEARALASKRRKIPNHTINPPKRAIAEVTNKVSPNVDAEGIYIAALKGDPIILSQLSGR 87

Query: 71  EASSDKNVSIYENTT--AQSEGDGSCFKCGKLGHWARDCDAPGGGGSFSSSGNDMSVPDK 130
            A+S     I  NTT  AQS+G GSCFKCGKLGHWARDCDAPG GGSFS+S ND SV +K
Sbjct: 88  IANSGNKGFISANTTAHAQSDGGGSCFKCGKLGHWARDCDAPGSGGSFSASENDTSVAEK 147

Query: 131 ACPCGSGICSVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDSASVANVVSYGSQN-PL 190
           +CPCG G+C VLTANTERNRGRKFYKCPVRQENGGCGFFEWCD+AS  N+++YGSQN   
Sbjct: 148 SCPCGRGVCVVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDNASATNIMTYGSQNRAS 207

Query: 191 SSSFSDLQCPCGAGSCKILTAKTGNNVGKQFYCCPSSQVS 227
           SSSFSDL+CPCGAGSC  LTAKTG NVG+QFY CPS Q +
Sbjct: 208 SSSFSDLKCPCGAGSCITLTAKTGENVGQQFYRCPSYQAT 247

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BLAST of CsaV3_4G007470 vs. NCBI nr
Match: XP_022136561.1 (DNA-binding protein HEXBP-like [Momordica charantia] >XP_022136562.1 DNA-binding protein HEXBP-like [Momordica charantia] >XP_022136563.1 DNA-binding protein HEXBP-like [Momordica charantia] >XP_022136564.1 DNA-binding protein HEXBP-like [Momordica charantia])

HSP 1 Score: 276.2 bits (705), Expect = 1.1e-70
Identity = 146/216 (67.59%), Postives = 160/216 (74.07%), Query Frame = 0


Query: 20  KVRNVPNPTIDLPKNAVAEVSHKVPLNADFLGTYIAPYKGSRAL-SQISGSEASS-DKNV 79
           K R +PN TID PK A+AE            G Y+A  +G   L SQISG  A+S  K V
Sbjct: 36  KRRRIPNHTIDPPKMAIAEEPE---------GLYMAALRGKPNLPSQISGGTANSRAKGV 95

Query: 80  S------IYENTTAQSEGDGSCFKCGKLGHWARDCDAPGGGGSFSSSGNDMSVPDKACPC 139
           +      +  N TAQSEG GSCFKCGKLGHWARDCDAPGGGGSF ++G D SV +KACPC
Sbjct: 96  AAANRGFVSANPTAQSEGGGSCFKCGKLGHWARDCDAPGGGGSFGTAGTDASVAEKACPC 155

Query: 140 GSGICSVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDSASVANVVSYGSQNPLSSS-F 199
           G G+C VLTANTERNRGRKFYKCPVRQENGGCGFFEWCDSAS  NV++YGSQN  SSS F
Sbjct: 156 GLGVCIVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDSASGTNVMTYGSQNRASSSLF 215

Query: 200 SDLQCPCGAGSCKILTAKTGNNVGKQFYCCPSSQVS 227
           SDL CPCGAGSC ILTAKTG NVG+QFY CPS Q S
Sbjct: 216 SDLMCPCGAGSCIILTAKTGENVGQQFYRCPSYQES 242

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BLAST of CsaV3_4G007470 vs. TAIR10
Match: AT3G42860.1 (zinc knuckle (CCHC-type) family protein)

HSP 1 Score: 142.5 bits (358), Expect = 3.5e-34
Identity = 78/178 (43.82%), Postives = 102/178 (57.30%), Query Frame = 0


Query: 51  GTYIAPYKGSRA--LSQISGSEASSDKNVSIYENTTAQSEGDGSCFKCGKLGHWARDCDA 110
           G Y+A  KGS++    Q   + AS  ++V++      +S+                    
Sbjct: 41  GPYMAALKGSKSDQWQQSPLNPASKSRSVAVTTGGFQRSD-------------------- 100

Query: 111 PGGGGSFSSSGNDMSVPDKACPCGSGICSVLTANTERNRGRKFYKCPVRQENGGCGFFEW 170
            GGGG       +   P+K+CPCG GIC +LT+NT +N GRKFYKCP R+ENGGCGFF+W
Sbjct: 101 -GGGG----VAGEQDFPEKSCPCGVGICLILTSNTPKNPGRKFYKCPNREENGGCGFFQW 160

Query: 171 CDS-----ASVANVVSYGSQNPLSSSFSDLQCPCGAGSCKILTAKTGNNVGKQFYCCP 222
           CD+      S     SYG+ N   + F D QCPCGAG C++LTAKTG NVG+QFY CP
Sbjct: 161 CDAVQSSGTSTTTSNSYGNGN--DTKFPDHQCPCGAGLCRVLTAKTGENVGRQFYRCP 191

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BLAST of CsaV3_4G007470 vs. TAIR10
Match: AT5G13920.1 (GRF zinc finger / Zinc knuckle protein)

HSP 1 Score: 89.0 bits (219), Expect = 4.6e-18
Identity = 47/130 (36.15%), Postives = 59/130 (45.38%), Query Frame = 0


Query: 91  GSCFKCGKLGHWARDCDAPGGGGSFSSSGNDMSVPDKACPCGSGICSVLTANTERNRGRK 150
           G CF+C + GHW  DC           S  D   P   CPCG G C +  ANT  N GRK
Sbjct: 9   GDCFRCRQAGHWINDCPL--------KSYTDDPPPAIQCPCGGGFCEIKVANTRENPGRK 68

Query: 151 FYKCPVRQENGGCGFFEWCDSASVANVVSYGSQNPLSSSFSDLQCPCGAGSCKILTAKTG 210
           FYKCP  Q    C FF+WCD  +  ++           +F+   C CGAG C+ +   +G
Sbjct: 69  FYKCPTAQ---NCCFFKWCDKVTDEDI-------KFRPAFTIPICSCGAGPCRRVKDVSG 117

Query: 211 NNVGKQFYCC 221
                   CC
Sbjct: 129 R---AYLICC 117

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BLAST of CsaV3_4G007470 vs. TAIR10
Match: AT5G63920.1 (topoisomerase 3alpha)

HSP 1 Score: 49.3 bits (116), Expect = 4.0e-06
Identity = 41/117 (35.04%), Postives = 60/117 (51.28%), Query Frame = 0


Query: 59  GSRALSQISGSEASSDKNVSIYENTTAQSEGDGSCFKCGKLGHWARDCDA--PGGGGSFS 118
           GSR+ ++ +   A S+   +I  + T QS     C  C + GH + +C +  P    S  
Sbjct: 739 GSRSQARRTPGTAPSN---NIQGSNTRQS---NVCIHCQQRGHASTNCPSRVPASRNSRP 798

Query: 119 SSGNDMSVPDK-AC-PCGSGICSVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDS 172
           ++ N  +     +C  CGS  C + TANTE NRGR+F+ CP +    GC FF W DS
Sbjct: 799 TATNPRNDESTVSCNTCGSQ-CVLRTANTEANRGRQFFSCPTQ----GCSFFAWEDS 844

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BLAST of CsaV3_4G007470 vs. TAIR10
Match: AT5G04280.1 (RNA-binding (RRM/RBD/RNP motifs) family protein with retrovirus zinc finger-like domain)

HSP 1 Score: 45.8 bits (107), Expect = 4.5e-05
Identity = 20/34 (58.82%), Postives = 24/34 (70.59%), Query Frame = 0


Query: 89  GDGSCFKCGKLGHWARDCDAPGGG-----GSFSS 118
           G+  CFKCG++GHWARDC + GGG     G FSS
Sbjct: 124 GEDECFKCGRVGHWARDCPSAGGGRGGPVGGFSS 157

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BLAST of CsaV3_4G007470 vs. TAIR10
Match: AT1G54930.1 (GRF zinc finger / Zinc knuckle protein)

HSP 1 Score: 44.7 bits (104), Expect = 1.0e-04
Identity = 16/31 (51.61%), Postives = 21/31 (67.74%), Query Frame = 0


Query: 192 DLQCPCGAGSCKILTAKTGNNVGKQFYCCPS 223
           D+ CPC AG C  +T+KT  N  ++FY CPS
Sbjct: 37  DIHCPCNAGPCNTVTSKTEKNPNRRFYTCPS 67

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BLAST of CsaV3_4G007470 vs. Swiss-Prot
Match: sp|Q9LVP1|TOP3A_ARATH (DNA topoisomerase 3-alpha OS=Arabidopsis thaliana OX=3702 GN=TOP3A PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 7.3e-05
Identity = 41/117 (35.04%), Postives = 60/117 (51.28%), Query Frame = 0


Query: 59  GSRALSQISGSEASSDKNVSIYENTTAQSEGDGSCFKCGKLGHWARDCDA--PGGGGSFS 118
           GSR+ ++ +   A S+   +I  + T QS     C  C + GH + +C +  P    S  
Sbjct: 739 GSRSQARRTPGTAPSN---NIQGSNTRQS---NVCIHCQQRGHASTNCPSRVPASRNSRP 798

Query: 119 SSGNDMSVPDK-AC-PCGSGICSVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDS 172
           ++ N  +     +C  CGS  C + TANTE NRGR+F+ CP +    GC FF W DS
Sbjct: 799 TATNPRNDESTVSCNTCGSQ-CVLRTANTEANRGRQFFSCPTQ----GCSFFAWEDS 844

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BLAST of CsaV3_4G007470 vs. Swiss-Prot
Match: sp|C7J0A2|TOP3A_ORYSJ (DNA topoisomerase 3-alpha OS=Oryza sativa subsp. japonica OX=39947 GN=TOP3A PE=3 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 2.1e-04
Identity = 22/35 (62.86%), Postives = 24/35 (68.57%), Query Frame = 0


Query: 136 CSVLTANTERNRGRKFYKCPVRQENGGCGFFEWCD 171
           C + TANTE NRGRKFYKC    +N  CGFF W D
Sbjct: 815 CVLRTANTEANRGRKFYKC----QNLACGFFAWED 845

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BLAST of CsaV3_4G007470 vs. Swiss-Prot
Match: sp|Q8RWN5|RZ1C_ARATH (Glycine-rich RNA-binding protein RZ1C OS=Arabidopsis thaliana OX=3702 GN=RZ1C PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 8.1e-04
Identity = 20/34 (58.82%), Postives = 24/34 (70.59%), Query Frame = 0


Query: 89  GDGSCFKCGKLGHWARDCDAPGGG-----GSFSS 118
           G+  CFKCG++GHWARDC + GGG     G FSS
Sbjct: 124 GEDECFKCGRVGHWARDCPSAGGGRGGPVGGFSS 157

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BLAST of CsaV3_4G007470 vs. TrEMBL
Match: tr|A0A0A0KVB6|A0A0A0KVB6_CUCSA (DNA topoisomerase OS=Cucumis sativus OX=3659 GN=Csa_4G062410 PE=3 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 8.6e-128
Identity = 225/226 (99.56%), Postives = 225/226 (99.56%), Query Frame = 0


Query: 1   MKSSSLQWRTAAEVVSLVKKVRNVPNPTIDLPKNAVAEVSHKVPLNADFLGTYIAPYKGS 60
           MKSSSLQWRTAAEVVSLVKKVRNVPNPTIDLPKNAVAEVSHKVPLNADFLGTYIAPYKGS
Sbjct: 1   MKSSSLQWRTAAEVVSLVKKVRNVPNPTIDLPKNAVAEVSHKVPLNADFLGTYIAPYKGS 60

Query: 61  RALSQISGSEASSDKNVSIYENTTAQSEGDGSCFKCGKLGHWARDCDAPGGGGSFSSSGN 120
           RALSQISGSEASSDKNVSIYENTTAQSEGDGSCFKCGKLGHWARDCDAPGGGGSFSSSGN
Sbjct: 61  RALSQISGSEASSDKNVSIYENTTAQSEGDGSCFKCGKLGHWARDCDAPGGGGSFSSSGN 120

Query: 121 DMSVPDKACPCGSGICSVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDSASVANVVSY 180
           DMSVPDKACPCGSGICSVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDSASVANVVSY
Sbjct: 121 DMSVPDKACPCGSGICSVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDSASVANVVSY 180

Query: 181 GSQNPLSSSFSDLQCPCGAGSCKILTAKTGNNVGKQFYCCPSSQVS 227
           GSQNPLSSSFSDLQCPCGAGSCKILTAKTGNNVGKQFYCCPSSQ S
Sbjct: 181 GSQNPLSSSFSDLQCPCGAGSCKILTAKTGNNVGKQFYCCPSSQAS 226

[Close]

BLAST of CsaV3_4G007470 vs. TrEMBL
Match: tr|A0A1S4DYV0|A0A1S4DYV0_CUCME (DNA topoisomerase OS=Cucumis melo OX=3656 GN=LOC103493251 PE=3 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 7.7e-60
Identity = 111/136 (81.62%), Postives = 119/136 (87.50%), Query Frame = 0


Query: 91  GSCFKCGKLGHWARDCDAPGGGGSFSSSGNDMSVPDKACPCGSGICSVLTANTERNRGRK 150
           G+CFKCGKLGHWARDCDAPGGGGSF+SSGND+SV +KA PCGSG+C VLTANTERNRGRK
Sbjct: 103 GACFKCGKLGHWARDCDAPGGGGSFNSSGNDVSVAEKARPCGSGVCLVLTANTERNRGRK 162

Query: 151 FYKCPVRQENGGCGFFEWCDSASVANVVSYGSQNPLSSSFSDLQCPCGAGSCKILTAKTG 210
           FY+C VRQENGGCGFFEWCDSA          QNPLSSSF DLQCPCGAGSC+ILTAK+ 
Sbjct: 163 FYRCLVRQENGGCGFFEWCDSAP--------GQNPLSSSFPDLQCPCGAGSCRILTAKSR 222

Query: 211 NNVGKQFYCCPSSQVS 227
           NNVGKQFYCCPSSQ S
Sbjct: 223 NNVGKQFYCCPSSQDS 230

[Close]

BLAST of CsaV3_4G007470 vs. TrEMBL
Match: tr|M5X3H0|M5X3H0_PRUPE (DNA topoisomerase OS=Prunus persica OX=3760 GN=PRUPE_3G190200 PE=3 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 2.1e-49
Identity = 115/235 (48.94%), Postives = 141/235 (60.00%), Query Frame = 0


Query: 11  AAEVVSLVKKVRNV-----PNPTIDLPKNAVAEVSHKVPLNADFLGTYIAPYKGSRALSQ 70
           AAE  +L  K R +     PN  +     A    +HK  L+++  G Y A  KGS+  + 
Sbjct: 23  AAEAQALASKRRRISIAPSPNSAVSFHSRA----NHKEGLDSEDGGIYTAALKGSQRAAP 82

Query: 71  ISGSEASSDKNVSI-YENTTAQSEGDGSCFKCGKLGHWARDCDAPGGGGSFSSSGND--- 130
                  S   V++        S G GSCFKCGK GHWARDCD  GGG   +S G D   
Sbjct: 83  EPPRGGFSTGRVNVGRTGNDGISAGGGSCFKCGKPGHWARDCDVTGGGYYGNSGGGDSSA 142

Query: 131 -MSVPDKACPCGSGICSVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDSASVANVVS- 190
            +SVP+K+CPCG G C VLTANTE+NRGRKFYKCP+RQENGGCGFFEWCD+AS  NV++ 
Sbjct: 143 SISVPEKSCPCGFGACMVLTANTEKNRGRKFYKCPLRQENGGCGFFEWCDNASGPNVMAS 202

Query: 191 --YGSQNPL--------SSSFSDLQCPCGAGSCKILTAKTGNNVGKQFYCCPSSQ 225
              GS +           SSF  L+CPCG G C ILTAKTG N+G QFY CP+++
Sbjct: 203 TGMGSGSATRTHSYAASDSSFPALECPCGGGLCLILTAKTGKNIGSQFYRCPANE 253

[Close]

BLAST of CsaV3_4G007470 vs. TrEMBL
Match: tr|W9SC70|W9SC70_9ROSA (DNA topoisomerase OS=Morus notabilis OX=981085 GN=L484_017710 PE=3 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 2.1e-49
Identity = 116/228 (50.88%), Postives = 143/228 (62.72%), Query Frame = 0


Query: 11  AAEVVSLVKKVRNVPNPTIDLPKNAVAEVSHKVPLNADFLGTYIAPYKGSRALSQIS--- 70
           A E  +L    R    P++    NAV     K    A   G Y+A  KG+++L + +   
Sbjct: 26  AVEADALASSKRRRITPSV----NAVVPNPKKEISAAADEGLYMAALKGNQSLVRQTTAG 85

Query: 71  --GSEASSDKNVSIYENTTAQSEGDG---SCFKCGKLGHWARDCD-APGGGG---SFSSS 130
             G      K V   ++    S G G   SCFKCGK GHWARDCD APGGGG       +
Sbjct: 86  RVGVPKGRVKAVDAEKDDVFGSSGSGAGNSCFKCGKSGHWARDCDSAPGGGGGQYGTHGA 145

Query: 131 GNDMSVPDKACPCGSGICSVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDSASVANVV 190
           G+D S+P+K+CPCG GIC+VLTANTE+NRGRKFYKCP+RQENGGCGFFEWCD+AS  N +
Sbjct: 146 GSDPSIPEKSCPCGLGICTVLTANTEKNRGRKFYKCPLRQENGGCGFFEWCDNASGNNSM 205

Query: 191 SYGSQNPLSSSFSDLQCPCGAGSCKILTAKTGNNVGKQFYCCPSSQVS 227
           + G+     SSF    CPCGAG C ILTAKTG N+G+QFY CP++Q S
Sbjct: 206 AGGNYTS-DSSFPGRPCPCGAGLCLILTAKTGQNIGQQFYRCPANQGS 248

[Close]

BLAST of CsaV3_4G007470 vs. TrEMBL
Match: tr|A0A2P5ER70|A0A2P5ER70_9ROSA (DNA topoisomerase OS=Trema orientalis OX=63057 GN=TorRG33x02_162390 PE=3 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 9.8e-47
Identity = 110/222 (49.55%), Postives = 139/222 (62.61%), Query Frame = 0


Query: 11  AAEVVSLVKKVRNVPNPTIDLPKNAVAEVSHKVPLNADFLGTYIAPYKGSRALSQISGSE 70
           AAE  +L  K R +     +    A   ++ +V  +AD  G Y+A  KG++ L  +    
Sbjct: 27  AAEANALASKRRRITPSAANAVVFASPTIAKQVSSSADD-GVYLAALKGNQTL--LLRPT 86

Query: 71  ASSDK---NVSIYENTTAQSEGDGSCFKCGKLGHWARDCDAPGGGGSFSSSGNDM---SV 130
           A+SD     V    +  A + G  SCFKCGK GHWARDCD                  S+
Sbjct: 87  AASDPLRGRVPKGRDDDAFAGGGDSCFKCGKSGHWARDCDXXXXXXXXXXXXXXXXXPSI 146

Query: 131 PDKACPCGSGICSVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDSASVANVVSYGSQN 190
           P+K+C CG GIC+VLTANTE+NRGRKFYKCP+RQENGGCGFFEWCD+ S AN ++ GS  
Sbjct: 147 PEKSCLCGLGICTVLTANTEKNRGRKFYKCPLRQENGGCGFFEWCDNVSGANSMAGGSYT 206

Query: 191 PLSSSFSDLQCPCGAGSCKILTAKTGNNVGKQFYCCPSSQVS 227
             +SS+  LQCPCGAG C +LTAKTGNNVG+QFY CP++Q S
Sbjct: 207 S-NSSYPGLQCPCGAGLCMVLTAKTGNNVGQQFYRCPANQGS 244

The following BLAST results are available for this feature:

BLAST of CsaV3_4G007470 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147743.1	1.3e-127	99.56	PREDICTED: DNA topoisomerase 3-alpha-like [Cucumis sativus] >KGN53505.1 hypothet...	[more]
XP_022931285.1	1.9e-78	70.00	cold shock domain-containing protein 3-like [Cucurbita moschata]	[more]
XP_023552737.1	7.3e-78	70.00	cold shock domain-containing protein 3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022984959.1	1.0e-76	68.18	cold shock domain-containing protein 3-like [Cucurbita maxima]	[more]
XP_022136561.1	1.1e-70	67.59	DNA-binding protein HEXBP-like [Momordica charantia] >XP_022136562.1 DNA-binding...	[more]

BLAST of CsaV3_4G007470 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G42860.1	3.5e-34	43.82	zinc knuckle (CCHC-type) family protein	[more]
AT5G13920.1	4.6e-18	36.15	GRF zinc finger / Zinc knuckle protein	[more]
AT5G63920.1	4.0e-06	35.04	topoisomerase 3alpha	[more]
AT5G04280.1	4.5e-05	58.82	RNA-binding (RRM/RBD/RNP motifs) family protein with retrovirus zinc finger-like...	[more]
AT1G54930.1	1.0e-04	51.61	GRF zinc finger / Zinc knuckle protein	[more]

BLAST of CsaV3_4G007470 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9LVP1\|TOP3A_ARATH	7.3e-05	35.04	DNA topoisomerase 3-alpha OS=Arabidopsis thaliana OX=3702 GN=TOP3A PE=1 SV=1	[more]
sp\|C7J0A2\|TOP3A_ORYSJ	2.1e-04	62.86	DNA topoisomerase 3-alpha OS=Oryza sativa subsp. japonica OX=39947 GN=TOP3A PE=3...	[more]
sp\|Q8RWN5\|RZ1C_ARATH	8.1e-04	58.82	Glycine-rich RNA-binding protein RZ1C OS=Arabidopsis thaliana OX=3702 GN=RZ1C PE...	[more]

BLAST of CsaV3_4G007470 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KVB6\|A0A0A0KVB6_CUCSA	8.6e-128	99.56	DNA topoisomerase OS=Cucumis sativus OX=3659 GN=Csa_4G062410 PE=3 SV=1	[more]
tr\|A0A1S4DYV0\|A0A1S4DYV0_CUCME	7.7e-60	81.62	DNA topoisomerase OS=Cucumis melo OX=3656 GN=LOC103493251 PE=3 SV=1	[more]
tr\|M5X3H0\|M5X3H0_PRUPE	2.1e-49	48.94	DNA topoisomerase OS=Prunus persica OX=3760 GN=PRUPE_3G190200 PE=3 SV=1	[more]
tr\|W9SC70\|W9SC70_9ROSA	2.1e-49	50.88	DNA topoisomerase OS=Morus notabilis OX=981085 GN=L484_017710 PE=3 SV=1	[more]
tr\|A0A2P5ER70\|A0A2P5ER70_9ROSA	9.8e-47	49.55	DNA topoisomerase OS=Trema orientalis OX=63057 GN=TorRG33x02_162390 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0006265	DNA topological change

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding
GO:0003917	DNA topoisomerase type I activity
GO:0008270	zinc ion binding
GO:0003676	nucleic acid binding

Vocabulary: **INTERPRO**
Term	Definition
IPR036875	Znf_CCHC_sf
IPR000380	Topo_IA
IPR010666	Znf_GRF
IPR001878	Znf_CCHC

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006265	DNA topological change
biological_process	GO:0000338	protein deneddylation
biological_process	GO:0044710	single-organism metabolic process
biological_process	GO:0044763	single-organism cellular process
biological_process	GO:0009314	response to radiation
biological_process	GO:0070646	protein modification by small protein removal
biological_process	GO:0051276	chromosome organization
biological_process	GO:0010212	response to ionizing radiation
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0016579	protein deubiquitination
biological_process	GO:0045893	positive regulation of transcription, DNA-templated
biological_process	GO:0009640	photomorphogenesis
biological_process	GO:0016571	histone methylation
biological_process	GO:0000724	double-strand break repair via homologous recombination
biological_process	GO:0044699	single-organism process
biological_process	GO:0050896	response to stimulus
biological_process	GO:0070647	protein modification by small protein conjugation or removal
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0003917	DNA topoisomerase type I activity
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003676	nucleic acid binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_4G007470.1	CsaV3_4G007470.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001878	Zinc finger, CCHC-type	SMART	SM00343	c2hcfinal6	coord: 92..108 e-value: 5.7E-5 score: 32.5
IPR001878	Zinc finger, CCHC-type	PFAM	PF00098	zf-CCHC	coord: 92..107 e-value: 1.3E-7 score: 31.3
IPR001878	Zinc finger, CCHC-type	PROSITE	PS50158	ZF_CCHC	coord: 93..106 score: 10.609
None	No IPR available	GENE3D	G3DSA:4.10.60.10		coord: 68..120 e-value: 1.5E-7 score: 33.2
None	No IPR available	PANTHER	PTHR11390:SF25	DNA TOPOISOMERASE	coord: 79..109
None	No IPR available	PANTHER	PTHR11390:SF25	DNA TOPOISOMERASE	coord: 116..231
IPR010666	Zinc finger, GRF-type	PFAM	PF06839	zf-GRF	coord: 128..171 e-value: 5.8E-16 score: 58.2 coord: 194..224 e-value: 3.3E-9 score: 36.6
IPR000380	DNA topoisomerase, type IA	PANTHER	PTHR11390	PROKARYOTIC DNA TOPOISOMERASE	coord: 116..231
IPR000380	DNA topoisomerase, type IA	PANTHER	PTHR11390	PROKARYOTIC DNA TOPOISOMERASE	coord: 79..109
IPR036875	Zinc finger, CCHC-type superfamily	SUPERFAMILY	SSF57756	Retrovirus zinc finger-like domains	coord: 84..111

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsaV3_4G007470	CSPI04G07240	Wild cucumber (PI 183967)	cpicucB213
CsaV3_4G007470	Cucsa.179950	Cucumber (Gy14) v1	cgycucB289
CsaV3_4G007470	CmaCh16G004810	Cucurbita maxima (Rimu)	cmacucB0393
CsaV3_4G007470	CmoCh16G005210	Cucurbita moschata (Rifu)	cmocucB0388
CsaV3_4G007470	Cp4.1LG14g03120	Cucurbita pepo (Zucchini)	cpecucB0266
CsaV3_4G007470	CsGy4G007130	Cucumber (Gy14) v2	cgybcucB171
CsaV3_4G007470	Carg11404	Silver-seed gourd	carcucB0689
CsaV3_4G007470	Cla005433	Watermelon (97103) v1	cucwmB323
CsaV3_4G007470	ClCG07G010840	Watermelon (Charleston Gray)	cucwcgB343
CsaV3_4G007470	Lsi07G008070	Bottle gourd (USVL1VR-Ls)	cuclsiB323
CsaV3_4G007470	Cla97C07G137580	Watermelon (97103) v2	cucwmbB329
CsaV3_4G007470	Bhi01G002391	Wax gourd	cucwgoB370

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsaV3_4G007470	Cucumber (Chinese Long) v3	cuccucB166
CsaV3_4G007470	Silver-seed gourd	carcucB0052
CsaV3_4G007470	Cucumber (Gy14) v2	cgybcucB176
CsaV3_4G007470	Cucurbita maxima (Rimu)	cmacucB0841
CsaV3_4G007470	Cucurbita moschata (Rifu)	cmocucB0560
CsaV3_4G007470	Cucurbita moschata (Rifu)	cmocucB0827
CsaV3_4G007470	Cucurbita pepo (Zucchini)	cpecucB0496
CsaV3_4G007470	Wild cucumber (PI 183967)	cpicucB226
CsaV3_4G007470	Bottle gourd (USVL1VR-Ls)	cuclsiB317
CsaV3_4G007470	Melon (DHL92) v3.5.1	cucmeB317
CsaV3_4G007470	Melon (DHL92) v3.6.1	cucmedB309
CsaV3_4G007470	Watermelon (Charleston Gray)	cucwcgB295