CsaV3_2G010500 (gene) Cucumber (Chinese Long) v3

NameCsaV3_2G010500
Typegene
OrganismCucumis sativus (Cucumber (Chinese Long) v3)
Description60S ribosomal protein L38
Locationchr2 : 7570147 .. 7570802 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGAATCTACCTTTTGTTTCTCTTGTTGGACAGCCAAAGCAAATCCATGAGATCAAAGACTTTCTTCTCACTGCTAGAAGAAAGGATGCCCGCTCGGTCAAAATCAAGAGGAGCAAAGATGTAGTCAAGTTTAAGGTTCGCTGCTCCAAGTACCTTTACACGCTCTGCGTCTTTGACTCGGAGAAGGCTGACAAATTGAAGCAGTCTCTTCCCCCAGGTATTCTCTTAAGCTTTGTTTAATTTTTTCTTATGAAAAGAAGCTCTGTTAAATTTATGTTCAGGATTTGGCCTTGATTGGTTTCAAGAATCCATGTGTTTGTTATTTTTATCATGAATTTAAATTTATACGTTTTCTTGTTTAACTGCACACATGTTAAAGCGTTTTAATTCCTACATATTTTTGGTTTAAATTGTTGTAGGTTTGAGTGTGCAAGATCTTTGAAGAAGTAGTGAGCGAATTTTGGAGATTTATATGGAAAAACAAGGGCTGAAGGAAACATTTGCCAGTTATTTTTCTTTCTTATTTTCAATCATCTGCTGTGTTGAATGTTCGGATTTTTATAGGGACGGCTGTTCTTGTCACGAGTAGGAGTTGGACTGAACTTTTAGCGTTTCTTAAATTTTATGAACCCATCTCAGATTTTGAACTGTGA

mRNA sequence

ATGCTGAATCTACCTTTTGTTTCTCTTGTTGGACAGCCAAAGCAAATCCATGAGATCAAAGACTTTCTTCTCACTGCTAGAAGAAAGGATGCCCGCTCGGTCAAAATCAAGAGGAGCAAAGATGTAGTCAAGTTTAAGGTTCGCTGCTCCAAGTACCTTTACACGCTCTGCGTCTTTGACTCGGAGAAGGCTGACAAATTGAAGCAGTCTCTTCCCCCAGGGACGGCTGTTCTTGTCACGAGTAGGAGTTGGACTGAACTTTTAGCGTTTCTTAAATTTTATGAACCCATCTCAGATTTTGAACTGTGA

Coding sequence (CDS)

ATGCTGAATCTACCTTTTGTTTCTCTTGTTGGACAGCCAAAGCAAATCCATGAGATCAAAGACTTTCTTCTCACTGCTAGAAGAAAGGATGCCCGCTCGGTCAAAATCAAGAGGAGCAAAGATGTAGTCAAGTTTAAGGTTCGCTGCTCCAAGTACCTTTACACGCTCTGCGTCTTTGACTCGGAGAAGGCTGACAAATTGAAGCAGTCTCTTCCCCCAGGGACGGCTGTTCTTGTCACGAGTAGGAGTTGGACTGAACTTTTAGCGTTTCTTAAATTTTATGAACCCATCTCAGATTTTGAACTGTGA

Protein sequence

MLNLPFVSLVGQPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSLPPGTAVLVTSRSWTELLAFLKFYEPISDFEL
BLAST of CsaV3_2G010500 vs. NCBI nr
Match: XP_022946384.1 (60S ribosomal protein L38 isoform X1 [Cucurbita moschata] >XP_022972863.1 60S ribosomal protein L38 isoform X1 [Cucurbita maxima] >XP_023545547.1 60S ribosomal protein L38 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023545638.1 60S ribosomal protein L38 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 132.1 bits (331), Expect = 1.1e-27
Identity = 65/67 (97.01%), Postives = 66/67 (98.51%), Query Frame = 0

Query: 11 GQPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQS 70
          GQPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQS
Sbjct: 3  GQPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQS 62

Query: 71 LPPGTAV 78
          LPPG +V
Sbjct: 63 LPPGLSV 69

BLAST of CsaV3_2G010500 vs. NCBI nr
Match: RDX61312.1 (60S ribosomal protein L38, partial [Mucuna pruriens])

HSP 1 Score: 130.2 bits (326), Expect = 4.1e-27
Identity = 64/67 (95.52%), Postives = 65/67 (97.01%), Query Frame = 0

Query: 12  QPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSL 71
           QPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSL
Sbjct: 52  QPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSL 111

Query: 72  PPGTAVL 79
           PPG  +L
Sbjct: 112 PPGRVIL 118

BLAST of CsaV3_2G010500 vs. NCBI nr
Match: XP_020237024.1 (60S ribosomal protein L38-like [Cajanus cajan])

HSP 1 Score: 129.8 bits (325), Expect = 5.3e-27
Identity = 65/77 (84.42%), Postives = 70/77 (90.91%), Query Frame = 0

Query: 1  MLNLPFVSLVGQPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFD 60
          +L++  V LV  PKQIHEIKDFLLTARRKDARSVKIKRS+DVVKFKVRCSKYLYTLCVFD
Sbjct: 19 LLSVTAVHLVTMPKQIHEIKDFLLTARRKDARSVKIKRSRDVVKFKVRCSKYLYTLCVFD 78

Query: 61 SEKADKLKQSLPPGTAV 78
           EKADKLKQSLPPG +V
Sbjct: 79 PEKADKLKQSLPPGLSV 95

BLAST of CsaV3_2G010500 vs. NCBI nr
Match: PKI38043.1 (hypothetical protein CRG98_041573 [Punica granatum])

HSP 1 Score: 129.4 bits (324), Expect = 7.0e-27
Identity = 64/67 (95.52%), Postives = 66/67 (98.51%), Query Frame = 0

Query: 12 QPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSL 71
          QPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSL
Sbjct: 11 QPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSL 70

Query: 72 PPGTAVL 79
          PPG+ +L
Sbjct: 71 PPGSFLL 77

BLAST of CsaV3_2G010500 vs. NCBI nr
Match: EEF47361.1 (60S ribosomal protein L38, putative [Ricinus communis])

HSP 1 Score: 129.4 bits (324), Expect = 7.0e-27
Identity = 64/66 (96.97%), Postives = 65/66 (98.48%), Query Frame = 0

Query: 12 QPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSL 71
          QPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSL
Sbjct: 10 QPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSL 69

Query: 72 PPGTAV 78
          PPG +V
Sbjct: 70 PPGLSV 75

BLAST of CsaV3_2G010500 vs. TAIR10
Match: AT2G43460.1 (Ribosomal L38e protein family)

HSP 1 Score: 124.4 bits (311), Expect = 4.1e-29
Identity = 60/65 (92.31%), Postives = 63/65 (96.92%), Query Frame = 0

Query: 13 PKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSLP 72
          PKQIHEIKDFLLTARRKDARSVKIKRSKD+VKFKVRCS+YLYTLCVFD EKADKLKQSLP
Sbjct: 2  PKQIHEIKDFLLTARRKDARSVKIKRSKDIVKFKVRCSRYLYTLCVFDQEKADKLKQSLP 61

Query: 73 PGTAV 78
          PG +V
Sbjct: 62 PGLSV 66

BLAST of CsaV3_2G010500 vs. TAIR10
Match: AT3G59540.1 (Ribosomal L38e protein family)

HSP 1 Score: 124.4 bits (311), Expect = 4.1e-29
Identity = 60/65 (92.31%), Postives = 63/65 (96.92%), Query Frame = 0

Query: 13 PKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSLP 72
          PKQIHEIKDFLLTARRKDARSVKIKRSKD+VKFKVRCS+YLYTLCVFD EKADKLKQSLP
Sbjct: 2  PKQIHEIKDFLLTARRKDARSVKIKRSKDIVKFKVRCSRYLYTLCVFDQEKADKLKQSLP 61

Query: 73 PGTAV 78
          PG +V
Sbjct: 62 PGLSV 66

BLAST of CsaV3_2G010500 vs. Swiss-Prot
Match: sp|O22860|RL38_ARATH (60S ribosomal protein L38 OS=Arabidopsis thaliana OX=3702 GN=RPL38A PE=3 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 7.3e-28
Identity = 60/65 (92.31%), Postives = 63/65 (96.92%), Query Frame = 0

Query: 13 PKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSLP 72
          PKQIHEIKDFLLTARRKDARSVKIKRSKD+VKFKVRCS+YLYTLCVFD EKADKLKQSLP
Sbjct: 2  PKQIHEIKDFLLTARRKDARSVKIKRSKDIVKFKVRCSRYLYTLCVFDQEKADKLKQSLP 61

Query: 73 PGTAV 78
          PG +V
Sbjct: 62 PGLSV 66

BLAST of CsaV3_2G010500 vs. Swiss-Prot
Match: sp|P46291|RL38_SOLLC (60S ribosomal protein L38 OS=Solanum lycopersicum OX=4081 GN=RPL38 PE=3 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 9.0e-26
Identity = 57/65 (87.69%), Postives = 62/65 (95.38%), Query Frame = 0

Query: 13 PKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSLP 72
          PKQIHEIKDFLLTARRKDAR+VKIK++KD+VKFKVRCSKYLYTLCV D EKADKLKQSLP
Sbjct: 2  PKQIHEIKDFLLTARRKDARTVKIKKNKDMVKFKVRCSKYLYTLCVSDFEKADKLKQSLP 61

Query: 73 PGTAV 78
          PG +V
Sbjct: 62 PGLSV 66

BLAST of CsaV3_2G010500 vs. Swiss-Prot
Match: sp|Q95V84|RL38_BRABE (60S ribosomal protein L38 OS=Branchiostoma belcheri OX=7741 GN=RPL38 PE=3 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 2.9e-24
Identity = 55/65 (84.62%), Postives = 60/65 (92.31%), Query Frame = 0

Query: 13 PKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSLP 72
          PKQIHEIKDFLLTARRKDA+SVKIK++KD VKFKVRCS+YLYTL + D EKADKLKQSLP
Sbjct: 2  PKQIHEIKDFLLTARRKDAKSVKIKKNKDNVKFKVRCSRYLYTLVITDKEKADKLKQSLP 61

Query: 73 PGTAV 78
          PG AV
Sbjct: 62 PGLAV 66

BLAST of CsaV3_2G010500 vs. Swiss-Prot
Match: sp|Q32PB9|RL38_BOVIN (60S ribosomal protein L38 OS=Bos taurus OX=9913 GN=RPL38 PE=3 SV=4)

HSP 1 Score: 105.1 bits (261), Expect = 4.6e-22
Identity = 51/65 (78.46%), Postives = 59/65 (90.77%), Query Frame = 0

Query: 13 PKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSLP 72
          P++I EIKDFLLTARRKDA+SVKIK++KD VKFKVRCS+YLYTL + D EKA+KLKQSLP
Sbjct: 2  PRKIEEIKDFLLTARRKDAKSVKIKKNKDNVKFKVRCSRYLYTLVITDKEKAEKLKQSLP 61

Query: 73 PGTAV 78
          PG AV
Sbjct: 62 PGLAV 66

BLAST of CsaV3_2G010500 vs. Swiss-Prot
Match: sp|P63173|RL38_HUMAN (60S ribosomal protein L38 OS=Homo sapiens OX=9606 GN=RPL38 PE=1 SV=2)

HSP 1 Score: 105.1 bits (261), Expect = 4.6e-22
Identity = 51/65 (78.46%), Postives = 59/65 (90.77%), Query Frame = 0

Query: 13 PKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSLP 72
          P++I EIKDFLLTARRKDA+SVKIK++KD VKFKVRCS+YLYTL + D EKA+KLKQSLP
Sbjct: 2  PRKIEEIKDFLLTARRKDAKSVKIKKNKDNVKFKVRCSRYLYTLVITDKEKAEKLKQSLP 61

Query: 73 PGTAV 78
          PG AV
Sbjct: 62 PGLAV 66

BLAST of CsaV3_2G010500 vs. TrEMBL
Match: tr|B9RM38|B9RM38_RICCO (60S ribosomal protein L38, putative OS=Ricinus communis OX=3988 GN=RCOM_1077580 PE=3 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 4.6e-27
Identity = 64/66 (96.97%), Postives = 65/66 (98.48%), Query Frame = 0

Query: 12 QPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSL 71
          QPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSL
Sbjct: 10 QPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSL 69

Query: 72 PPGTAV 78
          PPG +V
Sbjct: 70 PPGLSV 75

BLAST of CsaV3_2G010500 vs. TrEMBL
Match: tr|A0A2I0I2J4|A0A2I0I2J4_PUNGR (Uncharacterized protein OS=Punica granatum OX=22663 GN=CRG98_041573 PE=3 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 4.6e-27
Identity = 64/67 (95.52%), Postives = 66/67 (98.51%), Query Frame = 0

Query: 12 QPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSL 71
          QPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSL
Sbjct: 11 QPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSL 70

Query: 72 PPGTAVL 79
          PPG+ +L
Sbjct: 71 PPGSFLL 77

BLAST of CsaV3_2G010500 vs. TrEMBL
Match: tr|A0A2H5QP44|A0A2H5QP44_CITUN (Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_248250 PE=3 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 6.0e-27
Identity = 63/70 (90.00%), Postives = 67/70 (95.71%), Query Frame = 0

Query: 8  SLVGQPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKL 67
          +L+  PKQIHEIKDFLLTARRKDARSVKIKRS+DVVKFKVRCSKYLYTLCVFDSEKADKL
Sbjct: 27 ALIKMPKQIHEIKDFLLTARRKDARSVKIKRSRDVVKFKVRCSKYLYTLCVFDSEKADKL 86

Query: 68 KQSLPPGTAV 78
          KQSLPPG +V
Sbjct: 87 KQSLPPGLSV 96

BLAST of CsaV3_2G010500 vs. TrEMBL
Match: tr|A0A068VBT7|A0A068VBT7_COFCA (Uncharacterized protein OS=Coffea canephora OX=49390 GN=GSCOC_T00007069001 PE=3 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 6.0e-27
Identity = 64/70 (91.43%), Postives = 66/70 (94.29%), Query Frame = 0

Query: 8   SLVGQPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKL 67
           S +  PKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKL
Sbjct: 32  SSLNMPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKL 91

Query: 68  KQSLPPGTAV 78
           KQSLPPG +V
Sbjct: 92  KQSLPPGLSV 101

BLAST of CsaV3_2G010500 vs. TrEMBL
Match: tr|A0A200Q3X3|A0A200Q3X3_9MAGN (Ribosomal protein L38e OS=Macleaya cordata OX=56857 GN=BVC80_8895g26 PE=3 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.0e-26
Identity = 63/66 (95.45%), Postives = 65/66 (98.48%), Query Frame = 0

Query: 12 QPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSL 71
          +PKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSL
Sbjct: 15 EPKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDSEKADKLKQSL 74

Query: 72 PPGTAV 78
          PPG +V
Sbjct: 75 PPGLSV 80

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022946384.11.1e-2797.0160S ribosomal protein L38 isoform X1 [Cucurbita moschata] >XP_022972863.1 60S ri... [more]
RDX61312.14.1e-2795.5260S ribosomal protein L38, partial [Mucuna pruriens][more]
XP_020237024.15.3e-2784.4260S ribosomal protein L38-like [Cajanus cajan][more]
PKI38043.17.0e-2795.52hypothetical protein CRG98_041573 [Punica granatum][more]
EEF47361.17.0e-2796.9760S ribosomal protein L38, putative [Ricinus communis][more]
Match NameE-valueIdentityDescription
AT2G43460.14.1e-2992.31Ribosomal L38e protein family[more]
AT3G59540.14.1e-2992.31Ribosomal L38e protein family[more]
Match NameE-valueIdentityDescription
sp|O22860|RL38_ARATH7.3e-2892.3160S ribosomal protein L38 OS=Arabidopsis thaliana OX=3702 GN=RPL38A PE=3 SV=1[more]
sp|P46291|RL38_SOLLC9.0e-2687.6960S ribosomal protein L38 OS=Solanum lycopersicum OX=4081 GN=RPL38 PE=3 SV=1[more]
sp|Q95V84|RL38_BRABE2.9e-2484.6260S ribosomal protein L38 OS=Branchiostoma belcheri OX=7741 GN=RPL38 PE=3 SV=1[more]
sp|Q32PB9|RL38_BOVIN4.6e-2278.4660S ribosomal protein L38 OS=Bos taurus OX=9913 GN=RPL38 PE=3 SV=4[more]
sp|P63173|RL38_HUMAN4.6e-2278.4660S ribosomal protein L38 OS=Homo sapiens OX=9606 GN=RPL38 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
tr|B9RM38|B9RM38_RICCO4.6e-2796.9760S ribosomal protein L38, putative OS=Ricinus communis OX=3988 GN=RCOM_1077580 ... [more]
tr|A0A2I0I2J4|A0A2I0I2J4_PUNGR4.6e-2795.52Uncharacterized protein OS=Punica granatum OX=22663 GN=CRG98_041573 PE=3 SV=1[more]
tr|A0A2H5QP44|A0A2H5QP44_CITUN6.0e-2790.00Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_248250 PE=3 SV=1[more]
tr|A0A068VBT7|A0A068VBT7_COFCA6.0e-2791.43Uncharacterized protein OS=Coffea canephora OX=49390 GN=GSCOC_T00007069001 PE=3 ... [more]
tr|A0A200Q3X3|A0A200Q3X3_9MAGN1.0e-2695.45Ribosomal protein L38e OS=Macleaya cordata OX=56857 GN=BVC80_8895g26 PE=3 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003735structural constituent of ribosome
Vocabulary: Cellular Component
TermDefinition
GO:0005840ribosome
GO:0005622intracellular
Vocabulary: Biological Process
TermDefinition
GO:0006412translation
Vocabulary: INTERPRO
TermDefinition
IPR038464Ribosomal_L38e_sf
IPR002675Ribosomal_L38e
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042254 ribosome biogenesis
biological_process GO:0006412 translation
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0022618 ribonucleoprotein complex assembly
cellular_component GO:0005840 ribosome
cellular_component GO:0022625 cytosolic large ribosomal subunit
cellular_component GO:0005622 intracellular
molecular_function GO:0003735 structural constituent of ribosome
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0003723 RNA binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_2G010500.1CsaV3_2G010500.1mRNA


Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002675Ribosomal protein L38ePFAMPF01781Ribosomal_L38ecoord: 13..77
e-value: 2.4E-33
score: 113.9
IPR002675Ribosomal protein L38ePANTHERPTHR1096560S RIBOSOMAL PROTEIN L38coord: 13..77
IPR002675Ribosomal protein L38ePRODOMPD010361coord: 13..77
e-value: 3.0E-29
score: 313.0
IPR038464Ribosomal protein L38e superfamilyGENE3DG3DSA:3.30.720.90coord: 12..79
e-value: 2.5E-33
score: 116.0
NoneNo IPR availablePANTHERPTHR10965:SF3SUBFAMILY NOT NAMEDcoord: 13..77