CsaV3_2G002470 (gene) Cucumber (Chinese Long) v3 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsaV3_2G002470
Type	gene
Organism	Cucumis sativus (Cucumber (Chinese Long) v3)
Description	tRNA (guanine(37)-N1)-methyltransferase
Location	chr2 : 1027298 .. 1031286 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCCTTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGGTACGCTATGTTTTGTGTTATATTGTTTCGATTTCAATTAATCATTTGGGTAATGGCATTTAGCATTAGTGTTACTCATATATTGTTGTCGTTGAACTGTTCATCTTTCTTATCCGAGTTCATGTTTTTGAAAACTTATCAGAATTCTCCTGATATTTTTTAGTTTTTTTCTTTGAAAAGTATTCTTGTTATTAACTTTTGTTTAAAAGCAGACTTTTCTTTGGCCCTCAGTTCGTTTCTGTTGAAAAATTTTCAGGTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAATCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGGTAAAGATCTCCATCTCCGTCCTCATATTTTTTCTGTTAACTATAAGCTTGGATTGCAATTTCATGTTCTTTATGGAAACTCTTCTGTAGCACATTCTGTTCGCTGAAGTTCCAATTCTTGTTCATTGATTTTCTTCATGTTACCTTAGTGGCTTTCTGGCTCTGTTCATGCATTCAAGTTCTTAAATAGTTCTAAAATTATCGAGCGAGTAATTTTTTATCTTAAAACTTCAATTTCTGTAGGGTATAACCATCAACTTGAATGCATCTTTAGTTAGAAAAGTGTCTAAAAACAGTCTATATATCACATGAAACCAAATTAACGTCGTGTAACTAGGTAATTTTCATAAAAATAATCTCGTTTCCCTTTAAAAATCACTCATTTTTTCTCTTCCTTCTTTTTACCTTATTTTCTCAATGGAATTTTCCTCGATCATGTCCTTAGATGACACCATTTTTCTATCTTGTTGATTCTTTGAATGCTCAGAATTATCAGAAATTCCAGAAATGAAACTTGAAGAATTGAAAGGCTTATGCATGTTTGAACGAGTTCCCTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGGTATTTTCAATAATTCTTCCCTTTATCACACATTGTGCAAATTTTACTTTCACCTTAGGATTGCCTGTTGAACTCCAGATATTTATAGATGCCTTTAACTTTCGAGTTGCAAGTCTGAAGTGTTATGTAAGGCATATCTAGCATCATTTTTAATGATCCCATTCCCATGACTCAAAATCCAAACGTTTTTTACATTGTGACTGCACATGTGCTGATAGAATGTGGTTGTCTTTGCCTCTCATTTGATATTATAACTACATGTGATGTGACCGTTCTACATCAAGCGGTTGCAATTATAAGAACAATGTTCGATTTCTGAAAGATAGTTTAAACAATTCTGTGAGCGAATTTACCCGTCATTTGATGTGTTTGTATTGTACATACGTAACTAACTTACTGTGTGGTTGCTCTGTAGATCATATTCTCAAGAAGATTTTGCCTCCTGAAGTGGAAGTGCCTTCATCTTTTGAGACAATAGTTAAGTACTCTAACCCCTGCCATGTTCCACCTACGCTTTCCTTTCTACATTTTGTATCCATTTCCATATGCTCTTTATAGCTTTTATGTGCGTTCTTTTGTTGCTCTGTATTACATTTTGGTACCACTTGTTTATGTGACATTGGTTGTAACGTTGCTTTTTCATGTACAACTATTAACATTGAGGTAATTTTGTACTGTGATTTGCAGGTCACGTTGCTCACCTGAATATACATGACGAATTACTTCCATATAAGGATGTCATTGCGAAGGTTATTTATGATGTATGTCATCAGTATCTATTTACTTATTTTCCTAATTATTTTGAAATGTACATTGTGATTTATTAATCACTATAATTTAGCTGTTAGTTCATTTTTTTATTCTTAATTTTGTTTTACTCTCCCCTTCTTTTCCTCCGTGCATATGTTGGGGGTACCTTTTTAAGAAATTCAAATGTTCAGAATCGGATATTTAATATTTTTATCCTCCCCCTTCATCCAGCAATATGCACACACACACATTTTCCATATTGAAACTTTTTCTGTAACTATTTCTTTTTGTAATCTTTGCAGAAAAATTATCCCAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTAAAGCAATATGGGGCGACTTTTAAGCTCGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGCTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCATCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCGTTCATGTATATAATTTGGATGCTAGAAAGTTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAATCTGTCACCTCAATACTAAAAGCTTGTGAGAAGGGCATTGGAGGGACCAATCAAGAATCACCATTGGCAAATGGTATGCCGACTGGTATTATATTATTATTATCATTTAAAGAAAATTTCACATCGTCTTCAGGAGATTAGTGTAGATGATCTTAATTTTCTTTCTTGCTTCAGTTCAGGTGCAAGAAGTTCAAGATTATATTAAAATTAGCAATAAATCTGTAGAACAATCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCAGAAAGTTGTGAGAACGAGAAAGGTAACATACTCTACCATAGTTTGTGATTGAACAACCAAGACCGTTTCTTCCTATAATTTTTTTTGCAGATTTTTTTTAATGAAAATTTGCTTTGCAGACAATGGAATTGCTGATGATGCTTCCACTCGTGTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCAGTGTATCTCATGCTAGGACCTGGGAACACGTTGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGGTACGAATGACCTTTTTTCTTTTTAAATTTCATTTCACTTATTGCAGTTGTTTTAGCATATTTCTCTACATTTGTGTGTCAGAATGAAGAGTTAAAAGTTGGGGAAATAACAATGTTAGGAAAGGGAAACAATTACTTTTTAATGCTTCAAATATGAAGGGAAACCATAAACGATTTTTTCTCTCTTACTATCACCATGACAGATATTTTGGATATGTCATATTTCCTAATTCCTACCTTTCAAATGGAATTCTTTGTTGTAAGTTTTGTAGTCACTCTCTATACCAATTTTATGAGTTTCTTCAATCTCCTTTAGCTCTTGGGTGAGGGAGAATGCCTTATCTCCTCCACTCAATTATCTCAACTCGTGTTTTTTATCCATATCTATATTTTGCTTAGTTAACCAATGAAGTATATTTTGTTATTTTCTTGCCAGATGCATTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAACCAAGATTTTATCATTTCGGTATGTGTATACTAAATTACTATAGATCCTTATTTGTCTGTCTTCTTTTTGATAATTGTATTTCTTTCTCACCATTCTATTTGGATATCTGTCTTGAAAAAAGTTGTCTTTCTTCTCATACCATCCTCTTTTAACTGTTGCACTCCAGGAGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGTATTGCTTTGTGTGTGTGTGTATAAATGGAAACTACTTGTTTTTAACTCTTTTTTCTTTTTTGTTTTATCAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGCGTTGATGAAGACACGACTAGCTAA

mRNA sequence

ATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCCTTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGGTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAATCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGAATTATCAGAAATTCCAGAAATGAAACTTGAAGAATTGAAAGGCTTATGCATGTTTGAACGAGTTCCCTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGATCATATTCTCAAGAAGATTTTGCCTCCTGAAGTGGAAGTGCCTTCATCTTTTGAGACAATAGTTAAGTACTCTAACCCCTGCCATGTTCCACCTACGCTTTCCTTTCTACATTTTAAAAATTATCCCAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTAAAGCAATATGGGGCGACTTTTAAGCTCGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGCTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCATCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCGTTCATGTATATAATTTGGATGCTAGAAAGTTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAATCTGTCACCTCAATACTAAAAGCTTGTGAGAAGGGCATTGGAGGGACCAATCAAGAATCACCATTGGCAAATGGTATGCCGACTGTTCAGGTGCAAGAAGTTCAAGATTATATTAAAATTAGCAATAAATCTGTAGAACAATCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCAGAAAGTTGTGAGAACGAGAAAGACAATGGAATTGCTGATGATGCTTCCACTCGTGTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCAGTGTATCTCATGCTAGGACCTGGGAACACGTTGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGATGCATTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAACCAAGATTTTATCATTTCGGAGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGCGTTGATGAAGACACGACTAGCTAA

Coding sequence (CDS)

ATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCCTTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGGTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAATCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGAATTATCAGAAATTCCAGAAATGAAACTTGAAGAATTGAAAGGCTTATGCATGTTTGAACGAGTTCCCTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGATCATATTCTCAAGAAGATTTTGCCTCCTGAAGTGGAAGTGCCTTCATCTTTTGAGACAATAGTTAAGTACTCTAACCCCTGCCATGTTCCACCTACGCTTTCCTTTCTACATTTTAAAAATTATCCCAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTAAAGCAATATGGGGCGACTTTTAAGCTCGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGCTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCATCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCGTTCATGTATATAATTTGGATGCTAGAAAGTTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAATCTGTCACCTCAATACTAAAAGCTTGTGAGAAGGGCATTGGAGGGACCAATCAAGAATCACCATTGGCAAATGGTATGCCGACTGTTCAGGTGCAAGAAGTTCAAGATTATATTAAAATTAGCAATAAATCTGTAGAACAATCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCAGAAAGTTGTGAGAACGAGAAAGACAATGGAATTGCTGATGATGCTTCCACTCGTGTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCAGTGTATCTCATGCTAGGACCTGGGAACACGTTGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGATGCATTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAACCAAGATTTTATCATTTCGGAGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGCGTTGATGAAGACACGACTAGCTAA

Protein sequence

MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKVQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETIVKYSNPCHVPPTLSFLHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKACEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVDEDTTS

Homology

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BLAST of CsaV3_2G002470 vs. NCBI nr
Match: KGN60638.1 (hypothetical protein Csa_2G005350 [Cucumis sativus])

HSP 1 Score: 931.4 bits (2406), Expect = 1.3e-267
Identity = 469/501 (93.61%), Postives = 472/501 (94.21%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 VKYSNPCHV-------------PPTLSFLHFKNYPRIKTVVNKVGSITNEFRVPKFEILK 180
           +     CHV                   ++ KNYPRIKTVVNKVGSITNEFRVPKFEILK
Sbjct: 121 LL----CHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILK 180

Query: 181 GENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPA 240
           GENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPA
Sbjct: 181 GENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPA 240

Query: 241 AQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESV 300
           AQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESV
Sbjct: 241 AQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESV 300

Query: 301 TSILKACEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSS 360
           TSILKACEKGIGGTNQESPLAN    VQVQEVQDYIKISNKSVEQSRSADISVAVLKRSS
Sbjct: 301 TSILKACEKGIGGTNQESPLAN----VQVQEVQDYIKISNKSVEQSRSADISVAVLKRSS 360

Query: 361 ESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFL 420
           ESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFL
Sbjct: 361 ESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFL 420

Query: 421 DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPN 480
           DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPN
Sbjct: 421 DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPN 480

Query: 481 KAMYCLSFRLPEQCVDEDTTS 489
           KAMYCLSFRLPEQCVDEDTTS
Sbjct: 481 KAMYCLSFRLPEQCVDEDTTS 493

[Close]

BLAST of CsaV3_2G002470 vs. NCBI nr
Match: XP_011648665.1 (PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis sativus])

HSP 1 Score: 929.5 bits (2401), Expect = 4.8e-267
Identity = 467/495 (94.34%), Postives = 472/495 (95.35%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 VKYSNPCHVPPTLSF-------LHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
              ++       L +       ++ KNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV
Sbjct: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI
Sbjct: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240

Query: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA
Sbjct: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300

Query: 301 CEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENE 360
           CEKGIGGTNQESPLAN    VQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENE
Sbjct: 301 CEKGIGGTNQESPLAN----VQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENE 360

Query: 361 KDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGS 420
           KDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGS
Sbjct: 361 KDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGS 420

Query: 421 IKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCL 480
           IKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCL
Sbjct: 421 IKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCL 480

Query: 481 SFRLPEQCVDEDTTS 489
           SFRLPEQCVDEDTTS
Sbjct: 481 SFRLPEQCVDEDTTS 491

[Close]

BLAST of CsaV3_2G002470 vs. NCBI nr
Match: XP_008458074.1 (PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo])

HSP 1 Score: 891.7 bits (2303), Expect = 1.1e-255
Identity = 452/496 (91.13%), Postives = 466/496 (93.95%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 VKYSNPCHVPPTLSF-------LHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
              ++       L +       ++ KNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV
Sbjct: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI
Sbjct: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240

Query: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VYANDLNPDS+RYLKVNAEINKVSGRVHVYNLDARKFIS+LMIVPPNESNSE+VTSILKA
Sbjct: 241 VYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISRLMIVPPNESNSETVTSILKA 300

Query: 301 CEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENE 360
            E+GIGGTNQESPLAN    VQVQEVQD I+ISNK VE SRSADISVAVLKRSSESCENE
Sbjct: 301 SEEGIGGTNQESPLAN----VQVQEVQD-IEISNKFVEHSRSADISVAVLKRSSESCENE 360

Query: 361 KDNGIADDASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALHFLDAFRG 420
           KD+ IADDASTRVAGRRNGNGNKRMKGS +VSHARTWEH+DHVIMNLPASALHFLDAFRG
Sbjct: 361 KDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRG 420

Query: 421 SIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYC 480
           SIKKKYWKGSLPWIHCYCFMRANENQDFIIS AESALNANIQDPIFHRVRDVAPNKAMYC
Sbjct: 421 SIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYC 480

Query: 481 LSFRLPEQCVDEDTTS 489
           LSFRLPEQCV+EDTTS
Sbjct: 481 LSFRLPEQCVNEDTTS 491

[Close]

BLAST of CsaV3_2G002470 vs. NCBI nr
Match: XP_022157592.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Momordica charantia])

HSP 1 Score: 815.1 bits (2104), Expect = 1.3e-232
Identity = 405/491 (82.48%), Postives = 438/491 (89.21%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHL LWALRIPRELCKAAIKILNGYLID+ R+KPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKAAIKILNGYLIDRARVKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQ PELS+IPE KLEELKGLC  E VPYSLTLGYSYWGADHILK+ILPP VEVPSSFETI
Sbjct: 61  VQAPELSQIPEAKLEELKGLCEIELVPYSLTLGYSYWGADHILKQILPPRVEVPSSFETI 120

Query: 121 VKYSNPCHVPPTLSF-------LHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
              ++       L +       ++ KNYPRIKTVVNKVG+ITN+FRVPKFEIL GE+DMV
Sbjct: 121 GHIAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNKFRVPKFEILMGEHDMV 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGATFKLDY LVYWNSRLEHEH+RLVSLFQPGE+ICDMFAGIGPFAIPAAQKECI
Sbjct: 181 TEVKQYGATFKLDYGLVYWNSRLEHEHVRLVSLFQPGEIICDMFAGIGPFAIPAAQKECI 240

Query: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VY NDLNPDS++YLKVNAEINKVS RVHVYNLDARKFISQLMIVP NESNSESVTS+LKA
Sbjct: 241 VYGNDLNPDSVQYLKVNAEINKVSDRVHVYNLDARKFISQLMIVPDNESNSESVTSVLKA 300

Query: 301 CEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENE 360
           C+KGIGG+NQESP AN    VQV+EVQ+  KIS+++VE SRSADISV+VLKRSSESC  E
Sbjct: 301 CDKGIGGSNQESPSAN----VQVEEVQENSKISDETVEHSRSADISVSVLKRSSESC--E 360

Query: 361 KDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGS 420
           KD+GI DDAS  VAGR+  N NKRMKGS++ HARTWEHVDHVIMNLPASAL FLDAFRGS
Sbjct: 361 KDDGIVDDASISVAGRKKRNANKRMKGSNICHARTWEHVDHVIMNLPASALQFLDAFRGS 420

Query: 421 IKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCL 480
           I++KYWKGSLPWIHCYCFMRANENQDFIISEAESALN  IQDP+FHRVRDVAPNKAMYCL
Sbjct: 421 IRRKYWKGSLPWIHCYCFMRANENQDFIISEAESALNGKIQDPVFHRVRDVAPNKAMYCL 480

Query: 481 SFRLPEQCVDE 485
           SFRLPEQC +E
Sbjct: 481 SFRLPEQCFNE 485

[Close]

BLAST of CsaV3_2G002470 vs. NCBI nr
Match: XP_022157593.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X2 [Momordica charantia])

HSP 1 Score: 794.7 bits (2051), Expect = 1.8e-226
Identity = 396/491 (80.65%), Postives = 428/491 (87.17%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHL LWALRIPRELCKAAIKILNGYLID+ R+KPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKAAIKILNGYLIDRARVKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQ PELS+IPE KLEELKGLC  E VPYSLTLGYSYWGADHILK+ILPP VEVPSSFETI
Sbjct: 61  VQAPELSQIPEAKLEELKGLCEIELVPYSLTLGYSYWGADHILKQILPPRVEVPSSFETI 120

Query: 121 VKYSNPCHVPPTLSF-------LHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
              ++       L +       ++ KNYPRIKTVVNKVG+ITN+FRVPKFEIL GE+DMV
Sbjct: 121 GHIAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNKFRVPKFEILMGEHDMV 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGATFKLDY LVYWNSRLEHEH+RLVSLFQPGE+ICDMFAGIGPFAIPAAQKECI
Sbjct: 181 TEVKQYGATFKLDYGLVYWNSRLEHEHVRLVSLFQPGEIICDMFAGIGPFAIPAAQKECI 240

Query: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VY NDLNPDS++YLKVNAEINKVS RVHVYNLDARKFISQLMIVP NESNSESVTS+LKA
Sbjct: 241 VYGNDLNPDSVQYLKVNAEINKVSDRVHVYNLDARKFISQLMIVPDNESNSESVTSVLKA 300

Query: 301 CEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENE 360
           C+KGIGG+NQESP AN    VQV+EVQ+  KIS+++VE SRSADISV+VLKRSSESCE  
Sbjct: 301 CDKGIGGSNQESPSAN----VQVEEVQENSKISDETVEHSRSADISVSVLKRSSESCEK- 360

Query: 361 KDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGS 420
                        AGR+  N NKRMKGS++ HARTWEHVDHVIMNLPASAL FLDAFRGS
Sbjct: 361 -------------AGRKKRNANKRMKGSNICHARTWEHVDHVIMNLPASALQFLDAFRGS 420

Query: 421 IKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCL 480
           I++KYWKGSLPWIHCYCFMRANENQDFIISEAESALN  IQDP+FHRVRDVAPNKAMYCL
Sbjct: 421 IRRKYWKGSLPWIHCYCFMRANENQDFIISEAESALNGKIQDPVFHRVRDVAPNKAMYCL 473

Query: 481 SFRLPEQCVDE 485
           SFRLPEQC +E
Sbjct: 481 SFRLPEQCFNE 473

[Close]

BLAST of CsaV3_2G002470 vs. TAIR10
Match: AT3G56120.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 544.7 bits (1402), Expect = 6.0e-155
Identity = 276/492 (56.10%), Postives = 338/492 (68.70%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           M DESKFDV+L LWALRIPRELCK+A +ILNGY+++ PRIKP+TEDPTC+K R VILSE 
Sbjct: 1   MFDESKFDVNLKLWALRIPRELCKSASRILNGYMLNMPRIKPITEDPTCEKTRLVILSES 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           V+  +LSEIPE KL +LK L   E VP+S+TLGYSYW ADH+LK+ILP  +++PSSFETI
Sbjct: 61  VKNADLSEIPEEKLNQLKKLSELEVVPHSVTLGYSYWSADHLLKQILPDGLDIPSSFETI 120

Query: 121 VKYSNPCHVPPTLSF-------LHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
              ++       L F       ++ KNYPRIKT+VNKVG+I+NEFRVPKFE+L GEN M 
Sbjct: 121 GHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRVPKFEVLAGENGME 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGA FKLDY LVYWNSRLEHEH+RL SLF+PGE +CDMFAGIGPFAIPAAQK C 
Sbjct: 181 TEVKQYGARFKLDYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFAGIGPFAIPAAQKGCF 240

Query: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VYANDLNPDS+RYLK+NA+ NKV   + V+N+DARKF S LM V   E N +SV    K 
Sbjct: 241 VYANDLNPDSVRYLKINAKFNKVDDLICVHNMDARKFFSHLMAVSTCEDNLQSVADNDKT 300

Query: 301 CEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENE 360
            E  +           G                                       C++ 
Sbjct: 301 KEAAVS--------RGGETNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDCKSI 360

Query: 361 KDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGS 420
           +                 G+ NKR++ + +  A+ WEH+DHVIMNLPASAL FLD+F   
Sbjct: 361 E-----------------GHANKRLRQTLLPIAKPWEHIDHVIMNLPASALQFLDSFSNV 420

Query: 421 IKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCL 480
           I+KKYWKG LP IHCYCF+RA+E  +FII+EAE+AL  +I+DP+FH+VRDVAPNKAM+CL
Sbjct: 421 IQKKYWKGPLPLIHCYCFIRASETTEFIIAEAETALKFHIEDPVFHKVRDVAPNKAMFCL 467

Query: 481 SFRLPEQCVDED 486
           SFRLPE C+ ++
Sbjct: 481 SFRLPEACLKQE 467

[Close]

BLAST of CsaV3_2G002470 vs. TAIR10
Match: AT4G27340.1 (Met-10+ like family protein)

HSP 1 Score: 171.4 bits (433), Expect = 1.4e-42
Identity = 126/442 (28.51%), Postives = 187/442 (42.31%), Query Frame = 0


Query: 53  RYVILSEKVQTPELSEIPE------MKLEELKGLCMFERVPYSLTLGYSYWGADHILKKI 112
           R ++L EK    E+ ++PE       + +       FE V   LTL Y YW    IL+ +
Sbjct: 274 RLLLLDEKYSGEEVQDLPEAIKVLFAEAKMADASLSFELVKCRLTLFYDYWPMIEILEAV 333

Query: 113 LPPEVEVPSSFETI--VKYSN--PCHVP---PTLSFLHFKNYPRIKTVVNKVGSITNEFR 172
           LP  + VPS+FE +  + + N    H+P        +  KN P+I+TVVNK+  I N+FR
Sbjct: 334 LPKGMIVPSAFEMVGHIAHLNLRDEHLPYKRLIAKVVLDKNQPKIQTVVNKIDPIHNDFR 393

Query: 173 VPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAG 232
             + E+L G + +VT V + G  F +D + VYWNS+L  E  RL+  F   +V+CD+FAG
Sbjct: 394 TMQLEVLAGNHSLVTLVVENGLRFHVDLARVYWNSKLGTERQRLLLGFDQNDVVCDVFAG 453

Query: 233 IGPFAIPAAQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPP 292
           +GP A+ AA+    VYANDLNP ++ +++ N+ +NK+  R+ ++N+D R+FI  +     
Sbjct: 454 VGPIALAAARIVKRVYANDLNPHAVEFMEQNSVVNKLEKRIEIFNMDGRRFIKAMF---- 513

Query: 293 NESNSESVTSILKACEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADIS 352
                        + EKG                                          
Sbjct: 514 -------------SSEKG------------------------------------------ 573

Query: 353 VAVLKRSSESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNL 412
                                                             + V  V+MNL
Sbjct: 574 --------------------------------------------------QKVTQVVMNL 606

Query: 413 PASALHFLDAFRGSIKKKYWKG--SLPWIHCYCFMRANENQDFIISEAESALNANIQDPI 472
           P  A   LDAFRG    ++     S P IH Y F +A++ +         AL+    D  
Sbjct: 634 PKDAAESLDAFRGVYNDRHRDEGLSFPTIHVYGFSKASDPEFDFHERIRIALSEVAVDVK 606

Query: 473 FHRVRDVAPNKAMYCLSFRLPE 480
             +VR VAP K M C SF LP+
Sbjct: 694 MRKVRLVAPGKWMLCASFILPK 606

[Close]

BLAST of CsaV3_2G002470 vs. TAIR10
Match: AT4G04670.1 (Met-10+ like family protein / kelch repeat-containing protein)

HSP 1 Score: 57.0 bits (136), Expect = 3.8e-08
Identity = 38/115 (33.04%), Postives = 57/115 (49.57%), Query Frame = 0


Query: 156 NEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICD 215
           N  R    EIL G+N  V   ++ G  +  D +   ++     E +R+ ++    EV+ D
Sbjct: 785 NGTRDSTLEILVGDNGWVNH-RENGILYSFDATKCMFSWGNLSEKLRMGNMACENEVVVD 844

Query: 216 MFAGIGPFAIP--AAQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDAR 269
           +FAGIG F +P     K  +VYA + NP +I  L+ N E N VS R  +   D R
Sbjct: 845 LFAGIGYFVLPFLVRAKAKLVYACEWNPHAIEALRRNVEANSVSERCIILEGDNR 898

[Close]

BLAST of CsaV3_2G002470 vs. Swiss-Prot
Match: sp|F6HH45|TRM52_VITVI (tRNA (guanine(37)-N1)-methyltransferase 2 OS=Vitis vinifera OX=29760 GN=VIT_11s0016g04350 PE=3 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 2.1e-181
Identity = 324/503 (64.41%), Postives = 386/503 (76.74%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           +LDESKFD+ L LWALRIP +LCK A +ILNGYL+DKPR+KP+TEDPTC+ NRY+ILSE+
Sbjct: 2   LLDESKFDLQLKLWALRIPSQLCKVASRILNGYLLDKPRVKPITEDPTCETNRYIILSER 61

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           ++ P+LS+IPE K +ELK LC  E VPYSLTLGYSYW ADH+LK+ILP  VEVPSSFETI
Sbjct: 62  IKNPDLSDIPEDKRDELKVLCKIEVVPYSLTLGYSYWSADHVLKQILPDGVEVPSSFETI 121

Query: 121 VKYSNPCHV-------------PPTLSFLHFKNYPRIKTVVNKVGSITNEFRVPKFEILK 180
               + CH+                   ++ KNYPRIKTVVNKVG+ITNEFRVPKFEIL 
Sbjct: 122 T--FSLCHIAHLNITGELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNEFRVPKFEILV 181

Query: 181 GENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPA 240
           G++DMVTEVKQY ATFKLDYSLVYWNSRLEHEH+RLVS F+PG++ICDMF+G+GPFAIPA
Sbjct: 182 GKDDMVTEVKQYRATFKLDYSLVYWNSRLEHEHMRLVSQFRPGQIICDMFSGVGPFAIPA 241

Query: 241 AQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESV 300
           AQK C+VYANDLNPDSIRYLK+NA+INKV   +  YN+DARKFISQLM VP +E   E  
Sbjct: 242 AQKGCLVYANDLNPDSIRYLKINAKINKVDDNIWAYNMDARKFISQLMEVPVHEILPEPD 301

Query: 301 TSILKACEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRS-ADISVAVLKRS 360
             +LKA E+     N ++   NG  TV+   V      +   +E S + AD +VA +K+ 
Sbjct: 302 VPVLKATEECSIQANVQTESQNGGLTVEAIGVHSDDSSNMDGIEGSCTVADDTVAAVKKH 361

Query: 361 SESCENEKDNGIADDASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALH 420
           S SCE E  NG  D+A+  V GRR    NKRM+GS S S+ + WEHVDHVIMNLPASA+ 
Sbjct: 362 SGSCEEE--NGTYDNATVSVVGRRKDRMNKRMRGSESSSNIKPWEHVDHVIMNLPASAIQ 421

Query: 421 FLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVA 480
           FLDAFRG I++KYWKGSLPWIHCYCF+RANE  + IISEAE+ALNA+IQ+PI HRVRDVA
Sbjct: 422 FLDAFRGLIQRKYWKGSLPWIHCYCFIRANETNEMIISEAETALNASIQEPILHRVRDVA 481

Query: 481 PNKAMYCLSFRLPEQ-CVDEDTT 488
           PNKAM+CLSFRL E+ C  ED T
Sbjct: 482 PNKAMFCLSFRLSEEACFKEDAT 500

[Close]

BLAST of CsaV3_2G002470 vs. Swiss-Prot
Match: sp|Q93YU6|TRM51_ARATH (tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3g56120 PE=2 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 1.1e-153
Identity = 276/492 (56.10%), Postives = 338/492 (68.70%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           M DESKFDV+L LWALRIPRELCK+A +ILNGY+++ PRIKP+TEDPTC+K R VILSE 
Sbjct: 1   MFDESKFDVNLKLWALRIPRELCKSASRILNGYMLNMPRIKPITEDPTCEKTRLVILSES 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           V+  +LSEIPE KL +LK L   E VP+S+TLGYSYW ADH+LK+ILP  +++PSSFETI
Sbjct: 61  VKNADLSEIPEEKLNQLKKLSELEVVPHSVTLGYSYWSADHLLKQILPDGLDIPSSFETI 120

Query: 121 VKYSNPCHVPPTLSF-------LHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
              ++       L F       ++ KNYPRIKT+VNKVG+I+NEFRVPKFE+L GEN M 
Sbjct: 121 GHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRVPKFEVLAGENGME 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGA FKLDY LVYWNSRLEHEH+RL SLF+PGE +CDMFAGIGPFAIPAAQK C 
Sbjct: 181 TEVKQYGARFKLDYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFAGIGPFAIPAAQKGCF 240

Query: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VYANDLNPDS+RYLK+NA+ NKV   + V+N+DARKF S LM V   E N +SV    K 
Sbjct: 241 VYANDLNPDSVRYLKINAKFNKVDDLICVHNMDARKFFSHLMAVSTCEDNLQSVADNDKT 300

Query: 301 CEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENE 360
            E  +           G                                       C++ 
Sbjct: 301 KEAAVS--------RGGETNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDCKSI 360

Query: 361 KDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGS 420
           +                 G+ NKR++ + +  A+ WEH+DHVIMNLPASAL FLD+F   
Sbjct: 361 E-----------------GHANKRLRQTLLPIAKPWEHIDHVIMNLPASALQFLDSFSNV 420

Query: 421 IKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCL 480
           I+KKYWKG LP IHCYCF+RA+E  +FII+EAE+AL  +I+DP+FH+VRDVAPNKAM+CL
Sbjct: 421 IQKKYWKGPLPLIHCYCFIRASETTEFIIAEAETALKFHIEDPVFHKVRDVAPNKAMFCL 467

Query: 481 SFRLPEQCVDED 486
           SFRLPE C+ ++
Sbjct: 481 SFRLPEACLKQE 467

[Close]

BLAST of CsaV3_2G002470 vs. Swiss-Prot
Match: sp|C5XX79|TRM5_SORBI (tRNA (guanine(37)-N1)-methyltransferase OS=Sorghum bicolor OX=4558 GN=Sb04g025390 PE=3 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 2.9e-143
Identity = 266/485 (54.85%), Postives = 337/485 (69.48%), Query Frame = 0


Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKIL-NGYLIDKPRIKPVTEDPTCDKNRYVILSEK 61
           LDESKF+  L LWALRIPREL  A  ++L +GYL+DKPR+K V EDP  DKNR VILSEK
Sbjct: 4   LDESKFEQRLQLWALRIPRELSSAVTRLLRSGYLLDKPRVKTVVEDPEGDKNRLVILSEK 63

Query: 62  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 121
           +Q P+LS++P+ +L+ LK LC  + VPY+LTLGYSYW A H+    +          + +
Sbjct: 64  IQKPDLSDMPQQELDSLKQLCNVDVVPYTLTLGYSYWSAGHVAHLNIS---------DDL 123

Query: 122 VKYSNPCHVPPTLSFLHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYG 181
           + Y N          ++ KNYPRI+TV NKVG+ITNEFRVPKFEIL G+NDMVTEVKQYG
Sbjct: 124 LPYKN-----VIAKVIYDKNYPRIQTVANKVGTITNEFRVPKFEILAGKNDMVTEVKQYG 183

Query: 182 ATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLN 241
           ATFKLDY LVYWNSRL+HEHIRLVSLF+ G+VICDMFAGIGPFAIPAAQK C+VYANDLN
Sbjct: 184 ATFKLDYGLVYWNSRLDHEHIRLVSLFKKGDVICDMFAGIGPFAIPAAQKGCVVYANDLN 243

Query: 242 PDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKACEKGIGG 301
           PDS+ YL+ NA+INKV   +  YN+DAR F+  LM VP  E+ S+   +  ++  K  G 
Sbjct: 244 PDSVHYLRTNAKINKVDDYIFAYNMDARVFMQNLMTVPGLETGSDCQVAADESYPKE-GV 303

Query: 302 TNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENEKDNGIAD 361
              E+  +NG          D  + S                 +++SE CE++  +G  D
Sbjct: 304 PANENSSSNG-------NHNDVREGSXXXXXXXXXXXXXXXXXQQTSEECESDCQDG--D 363

Query: 362 DASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKKYWK 421
            + T+   RRN   NKR++G     ++ WEH DHV+MNLPASAL FLD F G ++KKYW 
Sbjct: 364 ASQTK---RRN---NKRVRGPGPPPSKPWEHFDHVLMNLPASALQFLDCFDGLVQKKYWT 423

Query: 422 GSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQ 481
           GSLPWIHCYCF+R++E+++ I+S   + LNA I +PIFHRVRDVAPNKAM+CLSF+LP +
Sbjct: 424 GSLPWIHCYCFIRSSESEESILS---NKLNAKIAEPIFHRVRDVAPNKAMFCLSFKLPME 455

Query: 482 CVDED 486
           C+ ED
Sbjct: 484 CLRED 455

[Close]

BLAST of CsaV3_2G002470 vs. Swiss-Prot
Match: sp|A9T6G5|TRM5_PHYPA (tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrella patens subsp. patens OX=3218 GN=PHYPADRAFT_141055 PE=3 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 6.0e-104
Identity = 204/477 (42.77%), Postives = 280/477 (58.70%), Query Frame = 0


Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKV 61
           +D+S F V L   ALRIP++       +L+ YL+D PR+K + EDP     R ++ SE +
Sbjct: 11  IDKSAFVVRLPRVALRIPKQKSHRVASLLSKYLLDMPRVKHIVEDPQDPSMRLLLFSEAI 70

Query: 62  QTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETIV 121
             P+L  + E K+  +K +   + V + + L YSYW  +HILK+ILP   EVPSSFETI 
Sbjct: 71  AQPDLEGVSEEKILAVKEIVPLDVVQHEVVLDYSYWPVEHILKEILPAGCEVPSSFETI- 130

Query: 122 KYSNPCHVPPTLSFLHFKNY---------PRIKTVVNKVGSITNEFRVPKFEILKGENDM 181
              +  H+      L +K           P++KTVVNKVG+ITNEFRVP+FEIL GE  +
Sbjct: 131 --GHIAHLNLREDLLTYKKIIAEVILDKNPKLKTVVNKVGTITNEFRVPEFEILAGEPSL 190

Query: 182 VTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKEC 241
           VTE+KQ+GATF+LDY +VYWNSRLE EH RL + F+PG+VI DMFAGIGPFAIPAAQ+ C
Sbjct: 191 VTEIKQHGATFRLDYGMVYWNSRLEGEHKRLFAQFKPGQVIVDMFAGIGPFAIPAAQQGC 250

Query: 242 IVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILK 301
            VYANDLNP S+++LK+N++INKV   +  +NLDAR+F+ +L  V   E     + S   
Sbjct: 251 AVYANDLNPTSVKFLKLNSDINKVGESIKAFNLDAREFMRKL--VTEEEGCVGEIASSTL 310

Query: 302 ACEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCEN 361
             E  + GT   S   N     +  ++    K   +  ++++ +++              
Sbjct: 311 IAENSLNGTRIPSSKENSSSLTEGSDMPSSKKPEERKAKKAKVSEV-------------- 370

Query: 362 EKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRG 421
                                   +MK   +S  + WEH DHV+MNLPASAL FLD   G
Sbjct: 371 -----------------------VKMK---ISDVKPWEHFDHVVMNLPASALEFLDVLNG 430

Query: 422 SIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKA 470
            + K  WKG++P +HCYCFMR+NE    I+ +AE+ L  +I DP  + VRDVAPNKA
Sbjct: 431 LLCKGRWKGTMPRVHCYCFMRSNETNADIVKKAETYLGGSIADPDVYVVRDVAPNKA 442

[Close]

BLAST of CsaV3_2G002470 vs. Swiss-Prot
Match: sp|F4NUJ6|TRM5_BATDJ (tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strain JAM81 / FGSC 10211) OX=684364 GN=TRM5 PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 2.2e-66
Identity = 158/492 (32.11%), Postives = 249/492 (50.61%), Query Frame = 0


Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKV 61
           L+ + F+    L AL+IP      A++ L  +++  PR++ + +DPT  K R ++L   +
Sbjct: 16  LNHALFESSHTLPALKIPAHASGIAMETLRSHILVAPRLRTIVDDPTDKKWRLLLLDPSM 75

Query: 62  QTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETIV 121
           +  E++++P   L+E       + V +++ L Y YW +D +L+ ILP E+E P +FET+ 
Sbjct: 76  EASEINDLPN-PLKEFALKHEAKLVKHTIELKYDYWTSDQVLRSILPDEMETPGAFETV- 135

Query: 122 KYSNPCHVPPTLSFLHFKNY---------PRIKTVVNKVGSITNEFRVPKFEILKGENDM 181
              +  H+     +  FK+            IKTVVNK+ +I + FR  + EIL G NDM
Sbjct: 136 --GHIAHLNLRDRYQPFKHIIGQVILDKSSHIKTVVNKLDNIDHTFRFFQMEILAGINDM 195

Query: 182 VTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQ-KE 241
             ++K+ G  F  D+S VYWNSRL+ EH R++ LF   ++ICD+FAG+GPFA+PAA+ K 
Sbjct: 196 NAKLKEGGCFFHFDFSKVYWNSRLQGEHDRIIKLFGQNDLICDVFAGVGPFALPAAKHKR 255

Query: 242 CIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 301
           C+V+ANDLNP S +YL  N ++NK+  R+  +N+D R+FI Q +     + N+ ++ + +
Sbjct: 256 CVVFANDLNPQSFKYLMENIKLNKLETRILPFNMDGRQFIKQSL----EDLNNPAIWNKI 315

Query: 302 KACEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCE 361
                      ++ P +N              K  N+ VE    A ++           +
Sbjct: 316 ----------TKQKPTSND-------------KKRNRKVESPTVAPLT-----------D 375

Query: 362 NEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFR 421
               +GI                                H  H +MNLPA+A+ FLDAF 
Sbjct: 376 QPAISGI-------------------------------RHFKHYVMNLPATAIEFLDAFH 434

Query: 422 G---SIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIF--HRVRDVAP 479
           G    ++       LP IHC+CF  A + +  +I   E  +   +   +   H VR VAP
Sbjct: 436 GLYSGMRDVIMDSDLPTIHCHCFSNAKDVKADVIERVERVIGMPLGSNLIMVHSVRTVAP 434

[Close]

BLAST of CsaV3_2G002470 vs. TrEMBL
Match: tr|A0A0A0LFW8|A0A0A0LFW8_CUCSA (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G005350 PE=3 SV=1)

HSP 1 Score: 931.4 bits (2406), Expect = 8.3e-268
Identity = 469/501 (93.61%), Postives = 472/501 (94.21%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 VKYSNPCHV-------------PPTLSFLHFKNYPRIKTVVNKVGSITNEFRVPKFEILK 180
           +     CHV                   ++ KNYPRIKTVVNKVGSITNEFRVPKFEILK
Sbjct: 121 LL----CHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILK 180

Query: 181 GENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPA 240
           GENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPA
Sbjct: 181 GENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPA 240

Query: 241 AQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESV 300
           AQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESV
Sbjct: 241 AQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESV 300

Query: 301 TSILKACEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSS 360
           TSILKACEKGIGGTNQESPLAN    VQVQEVQDYIKISNKSVEQSRSADISVAVLKRSS
Sbjct: 301 TSILKACEKGIGGTNQESPLAN----VQVQEVQDYIKISNKSVEQSRSADISVAVLKRSS 360

Query: 361 ESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFL 420
           ESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFL
Sbjct: 361 ESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFL 420

Query: 421 DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPN 480
           DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPN
Sbjct: 421 DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPN 480

Query: 481 KAMYCLSFRLPEQCVDEDTTS 489
           KAMYCLSFRLPEQCVDEDTTS
Sbjct: 481 KAMYCLSFRLPEQCVDEDTTS 493

[Close]

BLAST of CsaV3_2G002470 vs. TrEMBL
Match: tr|A0A1S3C7L3|A0A1S3C7L3_CUCME (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103497610 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 7.3e-256
Identity = 452/496 (91.13%), Postives = 466/496 (93.95%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 VKYSNPCHVPPTLSF-------LHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
              ++       L +       ++ KNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV
Sbjct: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI
Sbjct: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240

Query: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VYANDLNPDS+RYLKVNAEINKVSGRVHVYNLDARKFIS+LMIVPPNESNSE+VTSILKA
Sbjct: 241 VYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISRLMIVPPNESNSETVTSILKA 300

Query: 301 CEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENE 360
            E+GIGGTNQESPLAN    VQVQEVQD I+ISNK VE SRSADISVAVLKRSSESCENE
Sbjct: 301 SEEGIGGTNQESPLAN----VQVQEVQD-IEISNKFVEHSRSADISVAVLKRSSESCENE 360

Query: 361 KDNGIADDASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALHFLDAFRG 420
           KD+ IADDASTRVAGRRNGNGNKRMKGS +VSHARTWEH+DHVIMNLPASALHFLDAFRG
Sbjct: 361 KDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRG 420

Query: 421 SIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYC 480
           SIKKKYWKGSLPWIHCYCFMRANENQDFIIS AESALNANIQDPIFHRVRDVAPNKAMYC
Sbjct: 421 SIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYC 480

Query: 481 LSFRLPEQCVDEDTTS 489
           LSFRLPEQCV+EDTTS
Sbjct: 481 LSFRLPEQCVNEDTTS 491

[Close]

BLAST of CsaV3_2G002470 vs. TrEMBL
Match: tr|A0A2N9H8Q8|A0A2N9H8Q8_FAGSY (tRNA (guanine(37)-N1)-methyltransferase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS35856 PE=3 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 1.3e-199
Identity = 353/506 (69.76%), Postives = 399/506 (78.85%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHL LWALRIPRELCK A +ILNGYL+DK RIKP+TEDPTC+KNRYVILS+K
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKVATRILNGYLLDKARIKPITEDPTCEKNRYVILSDK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQ P+LS+IP+ KL+ELKG C  E VPYSLTLGYSYWGADH+LK+ILPP VEVPSSFETI
Sbjct: 61  VQNPDLSDIPDDKLDELKGSCKIEVVPYSLTLGYSYWGADHVLKQILPPGVEVPSSFETI 120

Query: 121 VKYSNP------------------CHVPPTLSFLHFKNYPRIKTVVNKVGSITNEFRVPK 180
           VKY                      H       ++ KNYPRIKTVVNKVG+ITNEFRVPK
Sbjct: 121 VKYLTTHSTQLGHIAHLNIHEELLPHKDVIAKVIYDKNYPRIKTVVNKVGTITNEFRVPK 180

Query: 181 FEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGP 240
           FEIL G+NDMVTEVKQYGATFKLDY LVYWNSRLEHEH+RLVS F+ GE ICDMFAGIGP
Sbjct: 181 FEILAGQNDMVTEVKQYGATFKLDYGLVYWNSRLEHEHMRLVSQFRAGETICDMFAGIGP 240

Query: 241 FAIPAAQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNES 300
           FAIPAAQK C+VYANDLNPDS RYLK+NAEINKV   V  YN+DAR FISQLM VP +E 
Sbjct: 241 FAIPAAQKGCVVYANDLNPDSTRYLKINAEINKVDDLVRAYNMDARNFISQLMAVPDSEI 300

Query: 301 NSESVTSILKACEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVE-QSRSADISVA 360
            +E      + CE+     N+ES   NG  TV+V+EV D +  + +     SR AD SV 
Sbjct: 301 KTEPDVPQCRTCEEYEIQVNEESKSENGRQTVEVKEVPDNVTSNLECARGSSRKADASVC 360

Query: 361 VLKRSSESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPA 420
            +KRSS +C  ++ NGIAD   T  +GRR G+ NKRM+GS +S+A+TWEHVDHVIMNLPA
Sbjct: 361 AVKRSSSNC--QEVNGIADGTGT--SGRRKGSTNKRMRGSDISNAKTWEHVDHVIMNLPA 420

Query: 421 SALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRV 480
           SAL FLDAFRG IK+KYWKGSLPWIHCYCFMRANE Q+FI+SEAESALNA+IQDPIFHRV
Sbjct: 421 SALQFLDAFRGIIKRKYWKGSLPWIHCYCFMRANETQEFIVSEAESALNASIQDPIFHRV 480

Query: 481 RDVAPNKAMYCLSFRLPEQCVDEDTT 488
           RDVAPNKAM+CLSFRLPE C +ED T
Sbjct: 481 RDVAPNKAMFCLSFRLPEACFNEDNT 502

[Close]

BLAST of CsaV3_2G002470 vs. TrEMBL
Match: tr|A0A2N9HHQ9|A0A2N9HHQ9_FAGSY (tRNA (guanine(37)-N1)-methyltransferase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS39614 PE=3 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 4.8e-191
Identity = 339/489 (69.33%), Postives = 386/489 (78.94%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHL LWALRIPRELCK A +ILNGYL+DK RIKP+TEDPTC+KNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKVATRILNGYLLDKARIKPITEDPTCEKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQ P+LS+IP+ KL+ELKG C  E VPYSLTLGYSYWGADH+LK+ILPP VEVPSSFETI
Sbjct: 61  VQNPDLSDIPDDKLDELKGSCKIEVVPYSLTLGYSYWGADHVLKQILPPGVEVPSSFETI 120

Query: 121 VKYSNPCHVPPTLSFLHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYG 180
           VK                K     K ++ K+  + NEFRVP FEIL G+NDMVTEVKQYG
Sbjct: 121 VK----------------KIIQESKLLLIKLVQLQNEFRVPNFEILAGQNDMVTEVKQYG 180

Query: 181 ATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLN 240
           ATFKLDY LVYWNSRLEHEH+RLVS F+ GE+ICDMFAGIGPF+IPAAQK C+VYANDLN
Sbjct: 181 ATFKLDYGLVYWNSRLEHEHMRLVSQFRAGEIICDMFAGIGPFSIPAAQKGCVVYANDLN 240

Query: 241 PDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKACEKGIGG 300
           PDS RYLK+NAEINKV   V  YN+DAR FISQLM VP +E  +E      + CE+    
Sbjct: 241 PDSTRYLKINAEINKVDDLVRAYNMDARNFISQLMAVPDSEIKTEPDVPQCRTCEEYEIQ 300

Query: 301 TNQESPLANGMPTVQVQEVQDYIKISNKSVE-QSRSADISV-AVLKRSSESCENEKDNGI 360
            N+ES   NG  TV+V+EV D +  + +     SR AD SV A +KRSS +C  ++ NG 
Sbjct: 301 VNEESKSENGRQTVEVKEVPDNVTSNLECARGSSRKADTSVSAAVKRSSSNC--QEVNGS 360

Query: 361 ADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKKY 420
           AD   T VAGRR G+ NKRM+GS +S+A+TWEHVDHVIMNLPASAL FLDAFRG IK+KY
Sbjct: 361 ADGTGTSVAGRRKGSTNKRMRGSEISNAKTWEHVDHVIMNLPASALQFLDAFRGIIKRKY 420

Query: 421 WKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLP 480
           WKGSLPWIHCYCFMRANE Q+FI+SEAESALNA+IQDPIFHRVRDVAPNKAM+CLSFRLP
Sbjct: 421 WKGSLPWIHCYCFMRANETQEFIVSEAESALNASIQDPIFHRVRDVAPNKAMFCLSFRLP 471

Query: 481 EQCVDEDTT 488
           E C +ED T
Sbjct: 481 EACFNEDNT 471

[Close]

BLAST of CsaV3_2G002470 vs. TrEMBL
Match: tr|A0A2P6RJN5|A0A2P6RJN5_ROSCH (tRNA (guanine(37)-N1)-methyltransferase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2g0091071 PE=3 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 7.7e-189
Identity = 336/501 (67.07%), Postives = 393/501 (78.44%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHL LWALRIPRELCK A +ILNGYL+DKPRIKP+TEDPTC++NRY+ILSE+
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKVATRILNGYLLDKPRIKPITEDPTCERNRYMILSER 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQ  +LS IP+  L ELK LC  E VPYSLTLGYSYWGADHILK+ILPP VEVPSSFETI
Sbjct: 61  VQDSDLSIIPDTILNELKDLCKIEVVPYSLTLGYSYWGADHILKQILPPGVEVPSSFETI 120

Query: 121 VKY-------SNPCHV----------PPTLSFLHFKNYPRIKTVVNKVGSITNEFRVPKF 180
           VKY       S+  H+                ++ KNYPRIKT+VNKVGSI+NEFRVPKF
Sbjct: 121 VKYSAHDSTWSHVAHLNIHDELIPYKDVIAKVIYDKNYPRIKTIVNKVGSISNEFRVPKF 180

Query: 181 EILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPF 240
           E+L GE DMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVS F+ GEVICDMFAGIGPF
Sbjct: 181 EVLAGEKDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSQFRAGEVICDMFAGIGPF 240

Query: 241 AIPAAQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESN 300
           AIPAAQK CIVYANDLNPDSI YLK+NAEINKV   V  YNLDAR+F+SQLM VP     
Sbjct: 241 AIPAAQKGCIVYANDLNPDSIHYLKINAEINKVGDHVRTYNLDAREFVSQLMAVPDCGKE 300

Query: 301 SESVTSILKACEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVE-QSRSADISVAV 360
             S  S+LK+CE      +QES   NGM  V+ +EV D +  +   ++  SR+ D SV  
Sbjct: 301 LNSDASMLKSCETCTNQGSQESESGNGMLNVEAKEVLDSVTSNVDGLQSSSRNTDASVPP 360

Query: 361 LKRSSESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPAS 420
           +KR ++ C++E  NGI +  +  VAGR  GN NKR++GS + + +TWEHVDHVIMNLPAS
Sbjct: 361 VKRPADICQSE--NGIVNGTNVLVAGRSKGNKNKRLRGSEMINVKTWEHVDHVIMNLPAS 420

Query: 421 ALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVR 480
           AL FLDAFRG I++KYW+ +LP IHCYCF+RA+E Q++IISEAE+AL A+I+DPIFHRVR
Sbjct: 421 ALEFLDAFRGVIQRKYWRETLPLIHCYCFIRASETQEYIISEAEAALKAHIKDPIFHRVR 480

Query: 481 DVAPNKAMYCLSFRLPEQCVD 484
           DVAPNKAM+CLSFRLPE C++
Sbjct: 481 DVAPNKAMFCLSFRLPEACLN 499

The following BLAST results are available for this feature:

BLAST of CsaV3_2G002470 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN60638.1	1.3e-267	93.61	hypothetical protein Csa_2G005350 [Cucumis sativus]	[more]
XP_011648665.1	4.8e-267	94.34	PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis sativus]	[more]
XP_008458074.1	1.1e-255	91.13	PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo]	[more]
XP_022157592.1	1.3e-232	82.48	tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Momordica charantia]	[more]
XP_022157593.1	1.8e-226	80.65	tRNA (guanine(37)-N1)-methyltransferase 2 isoform X2 [Momordica charantia]	[more]

BLAST of CsaV3_2G002470 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G56120.1	6.0e-155	56.10	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT4G27340.1	1.4e-42	28.51	Met-10+ like family protein	[more]
AT4G04670.1	3.8e-08	33.04	Met-10+ like family protein / kelch repeat-containing protein	[more]

BLAST of CsaV3_2G002470 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|F6HH45\|TRM52_VITVI	2.1e-181	64.41	tRNA (guanine(37)-N1)-methyltransferase 2 OS=Vitis vinifera OX=29760 GN=VIT_11s0...	[more]
sp\|Q93YU6\|TRM51_ARATH	1.1e-153	56.10	tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3...	[more]
sp\|C5XX79\|TRM5_SORBI	2.9e-143	54.85	tRNA (guanine(37)-N1)-methyltransferase OS=Sorghum bicolor OX=4558 GN=Sb04g02539...	[more]
sp\|A9T6G5\|TRM5_PHYPA	6.0e-104	42.77	tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrella patens subsp. patens O...	[more]
sp\|F4NUJ6\|TRM5_BATDJ	2.2e-66	32.11	tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strai...	[more]

BLAST of CsaV3_2G002470 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LFW8\|A0A0A0LFW8_CUCSA	8.3e-268	93.61	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G0053...	[more]
tr\|A0A1S3C7L3\|A0A1S3C7L3_CUCME	7.3e-256	91.13	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103497610 ...	[more]
tr\|A0A2N9H8Q8\|A0A2N9H8Q8_FAGSY	1.3e-199	69.76	tRNA (guanine(37)-N1)-methyltransferase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS...	[more]
tr\|A0A2N9HHQ9\|A0A2N9HHQ9_FAGSY	4.8e-191	69.33	tRNA (guanine(37)-N1)-methyltransferase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS...	[more]
tr\|A0A2P6RJN5\|A0A2P6RJN5_ROSCH	7.7e-189	67.07	tRNA (guanine(37)-N1)-methyltransferase OS=Rosa chinensis OX=74649 GN=RchiOBHm_C...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0009019	tRNA (guanine-N1-)-methyltransferase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0030488	tRNA methylation

Vocabulary: **INTERPRO**
Term	Definition
IPR029063	SAM-dependent_MTases
IPR025792	tRNA_Gua_MeTrfase_euk
IPR030382	MeTrfase_TRM5/TYW2

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030488	tRNA methylation
biological_process	GO:0048449	floral organ formation
biological_process	GO:0016570	histone modification
biological_process	GO:0009909	regulation of flower development
biological_process	GO:0009908	flower development
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005759	mitochondrial matrix
cellular_component	GO:0005634	nucleus
molecular_function	GO:0009019	tRNA (guanine-N1-)-methyltransferase activity
molecular_function	GO:0052906	tRNA (guanine(37)-N(1))-methyltransferase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_2G002470.1	CsaV3_2G002470.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR030382	SAM-dependent methyltransferase TRM5/TYW2-type	PFAM	PF02475	Met_10	coord: 143..429 e-value: 1.7E-54 score: 184.5
IPR030382	SAM-dependent methyltransferase TRM5/TYW2-type	PROSITE	PS51684	SAM_MT_TRM5_TYW2	coord: 117..479 score: 88.612
None	No IPR available	GENE3D	G3DSA:3.40.50.150		coord: 132..341 e-value: 1.3E-46 score: 161.1
None	No IPR available	GENE3D	G3DSA:3.40.50.150		coord: 342..478 e-value: 9.6E-17 score: 63.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 351..382
None	No IPR available	PANTHER	PTHR23245	UNCHARACTERIZED	coord: 2..482
None	No IPR available	PANTHER	PTHR23245:SF36	TRNA GUANINE 37 -N1 -METHYLTRANSFERASE	coord: 2..482
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 213..270 e-value: 1.79956E-4 score: 38.9503
IPR025792	tRNA (guanine(37)-N(1))-methyltransferase, eukaryotic	HAMAP	MF_03152	TRM5	coord: 2..479 score: 22.21
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	SSF53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 138..271 coord: 387..479

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsaV3_2G002470	CSPI02G01360	Wild cucumber (PI 183967)	cpicucB080
CsaV3_2G002470	Cucsa.116210	Cucumber (Gy14) v1	cgycucB156
CsaV3_2G002470	CmaCh12G012350	Cucurbita maxima (Rimu)	cmacucB0202
CsaV3_2G002470	CmoCh12G012640	Cucurbita moschata (Rifu)	cmocucB0185
CsaV3_2G002470	Cp4.1LG07g10020	Cucurbita pepo (Zucchini)	cpecucB1015
CsaV3_2G002470	CsGy2G001370	Cucumber (Gy14) v2	cgybcucB064
CsaV3_2G002470	Carg26002	Silver-seed gourd	carcucB0307
CsaV3_2G002470	Cla016749	Watermelon (97103) v1	cucwmB174
CsaV3_2G002470	MELO3C004542	Melon (DHL92) v3.5.1	cucmeB142
CsaV3_2G002470	ClCG11G002640	Watermelon (Charleston Gray)	cucwcgB112
CsaV3_2G002470	Lsi11G002790	Bottle gourd (USVL1VR-Ls)	cuclsiB108
CsaV3_2G002470	MELO3C004542.2	Melon (DHL92) v3.6.1	cucmedB135
CsaV3_2G002470	Cla97C11G209100	Watermelon (97103) v2	cucwmbB108
CsaV3_2G002470	Bhi06G001644	Wax gourd	cucwgoB138

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsaV3_2G002470	Cucumber (Chinese Long) v3	cuccucB009
CsaV3_2G002470	Silver-seed gourd	carcucB0045
CsaV3_2G002470	Silver-seed gourd	carcucB0147
CsaV3_2G002470	Silver-seed gourd	carcucB0605
CsaV3_2G002470	Cucurbita maxima (Rimu)	cmacucB0934
CsaV3_2G002470	Cucumber (Gy14) v2	cgybcucB019
CsaV3_2G002470	Cucurbita maxima (Rimu)	cmacucB0081
CsaV3_2G002470	Cucurbita maxima (Rimu)	cmacucB0135
CsaV3_2G002470	Cucurbita moschata (Rifu)	cmocucB0070
CsaV3_2G002470	Cucurbita moschata (Rifu)	cmocucB0120
CsaV3_2G002470	Cucurbita moschata (Rifu)	cmocucB0924
CsaV3_2G002470	Cucurbita pepo (Zucchini)	cpecucB0114
CsaV3_2G002470	Cucurbita pepo (Zucchini)	cpecucB0450
CsaV3_2G002470	Cucurbita pepo (Zucchini)	cpecucB0826
CsaV3_2G002470	Wild cucumber (PI 183967)	cpicucB023
CsaV3_2G002470	Bottle gourd (USVL1VR-Ls)	cuclsiB142
CsaV3_2G002470	Melon (DHL92) v3.5.1	cucmeB116
CsaV3_2G002470	Melon (DHL92) v3.6.1	cucmedB112
CsaV3_2G002470	Watermelon (Charleston Gray)	cucwcgB156
CsaV3_2G002470	Watermelon (97103) v1	cucwmB180
CsaV3_2G002470	Watermelon (97103) v2	cucwmbB143
CsaV3_2G002470	Wax gourd	cucwgoB219