CSPI02G01360 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI02G01360
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	tRNA (guanine(37)-N1)-methyltransferase
Location	Chr2 : 920748 .. 925108 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCGACAGTTCCCGCTGTTAGTCTTCTACTACGGTGAAAATCTCGATTCTGATTCTGATTCTTTCTTTTCAGTGGAAAAATCTCCGAACCCATTACTCTACATTGTTCTATTCTGATTTGAATTTGTTTGCATTGAGTTGGGTGTAATTTTTGTTGTGTTATTGAGTATTGAGAAAAATCGAACTTGTGAATCTATCAATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCCTTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGGTACGCTATGTTTTGTGTTATATTGTTTCGATTTCAATTAATCATTTGGGTAATGGCATTTAGCATTAGTGTTACTCATATACTGTTGTCGTTGAACTGTTCATCTTTCTTATCCGAGTTCATGTTTTTGAAAACTTATCAGAATTCTCCTGATATTTTTTAGTTTTTTTCTTTGAAAAGTATTCTTGTTATTAACTTTTGTTTAAAAGCAGACTTTTCTTTGGCCCTCAGTTCGTTTCTGTTGAAAAATTTTCAGGTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAATCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGGTAAAGATCTCCATCTCCGTCCTCATATTTTTTCTGTTAACTATAAGCTTGGATTGCAATTTCATGTTCTTTATGGAAACTCTTCTGTAGCACATTCTGTTCGCTGAAGTTCCAATTCTTGTTCATTGATTTTCTTCATGTTACCTTAGTGGCTTTCTAGCTCTGTTCATGCATTCAAGTTCTTAAATAGTTCTAAAATTATCGAGCGAGTAATTTTTTATCTTAAAACTTCAATTTCTGTAGGGTATAACCATCAACTTGAATGCATCTTTAGTTAGAAAAGTGTCTAAAAACAGCCTATATATCACATGAAACCAAATTAACGTCGTGTAACTAGGTAATTTTCATAAAAATAATCTCGTTTCCCTTTAAAAATCAATCATTTTTTCTCTTCCTTCTTTTTACCTTATTTTGTCAATGGAATTTTCCTCGATCATGTCCTTAGATGACACCATTTTTCTATCTTGTTGATTCTTTGAATGCTCAGAATTATCAGAAATTCCAGAAATGAAACTTGAAGAATTGAAAGGCTTATGCATGTTTGAACGAGTTCCCTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGGTATTTTCAATAATTCTTCCCTTTATCACACATTGTGCAAATTTTACTTTCACCTTAGGATTGCCTGTTGAACTCCAGATATTTATAGATGCCTTTAACTTTCGAGTTGCAAGTCTGAAGTGTTATGTAAGGCATATCTAGCATCATTTTTAATGATCCCATTCCCATTACTCAAAATCCAAACGTTTTTTACATTGTGACTGCACATGTGCTGATAGAATGTGGTTGTCTTTGCCTCTCATTTGATATTATAACTACATGTGATGTGACCGTTCTACATCAAGCGGTTGCAATTATAAGAACAATGTTCGATTTCTGAAAGATAGTTTAAACAATTCTGTGAGCGAATTTACCCGTCATTTGATGTGTTTGTATTGTACTTACGTAACTAACTTACTGTGTGGTTGCTCTGTAGATCATATTCTCAAGAAGATTTTGCCTCCTGAAGTGGAAGTGCCTTCATCTTTTGAGACAATAGTTAAGTACTCTAACCCCTGCCATGTTCCACCTACGTTTTCCTTTCTACATTTTGTATCCATTTCCATATGCTCTTTATAGCTTTTATGTGCGTTCTTTTGTTGCTCTGTATTACATTTTGGTACCACTTGTTTATGTGACATTGGTTGTAACGTTGCTTTTTCATGTACAACTATTGACATTGAGGTAATTTCGTACTGTGATTTGCAGGTCACGTTGCTCACCTGAATATACATGACGAATTGCTTCCATATAAGGATGTCATTGCGAAGGTTATTTATGATGTATGTCATCAGTATCTATTTACTCATTTTCCTAATTATTTTGAAATGTACATTGTGATTTATTAATCACTATAATTTAGCTGTTAGTTCATTTTTTTATTCTTAATTTTGTTTTACTCTCCCCTTCTTTTCCTCCGTGCATATGTTGGGGCTACCTTTTTAAGAAATTCAAATGTTCAGAATCGGATATTTAATATTTTTATCCTCCCCCTTCATCCAGCAATATGCACACACACACATTTTCCATATTGAAACTTTTTCTGTAACTATTTCTTTTTGTAATCTTTGCAGAAAAATTATCCGAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTAAAGCAATATGGGGCAACTTTTAAGCTCGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGCTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCGTCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCGTTCATGTATATAATTTGGATGCTAGGAAGTTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAATCTGTCACCTCAATACTAAAAGCTTGTGAGAAGGGCATTGGAGGGACCAATCAAGAATCACCATCGGCAAATGGTATGCCGACTGGTATTATATTATTATTATCATTTAAAGAAAATTTCACATCGTCTTCAGGAGATTAGTGTAGATGATCTTAATTTTCTTTCTTGCTTCAGTTCAGGTGCAAGAAGTTCAAGATTATATTAAAATTAGCAATAAATCTGTAGAACACTCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCAGAAAGTTGTGAGAACGAGAAAGGTAACATACTCTACCATAGTTTGTGATTGAACAACCAAGACCGTTTCTTCCTATAATTTTTTTTGCAGATTTTTTTTAATGAAAATTTGCTTTGCAGACAATGGAATTGCTGATGATGCTTCCACTCGTGTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCAGTGTATCTCATGCTAGGACCTGGGAACACGTTGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGGTACGAATGACCTTTTTTCTTTTTAAATTTCATTTCACTTATTGCAGTTGTTTTAGCATATTTCTCTACATTTGTGTGTCAGAATGAAGAGTTAAAAGTTGGGGAAATAACAATGTTAGGAAAGGGAAACAATTACTTTTTAATGCTTCAAATATGAAGGGAAACCATAAACGATTTTTTCTCTCTTACTATCACCATGACAGATATTTTGGATATGTCATATTTCCTAATTCCTACCTTTCAAATGGAATTCTTTGTTGTAAGTTTTGTAGTCACTCTCTATACCAATTTTATGAGTTTCTTCAATCTCCTTTAGCTCTTGGGTGAGGGAGAATGCCTTATCTCCTCCACTCAATTATCTCAACTCGTGTTTTTTATCCATATCTATATTTTGCTTAGTTAACCAATGAAGTATATTTTGTTATTTTCTTGCCAGATGCATTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAACCAAGATTTTATCATTTCGGTATGTTTATACTAAATTACTATAGATCCTTATTTGTCTGTCTTCTTTTTGATAATTGTATTTCTTTCTCACCATTCTATTTGGATATCTGTCTTGAAAAAAGTTGTCTTTCTTCTCATACCATCCTCTTTTAACTGTTCCACTCCAGGAGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGTATTGCTTTGTGTGTGTGTGTATAAATGGAAACTACTTGTTTTTAACTCTTTTTTCTTTTTTGTTTTATCAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGCGTTGATGAAGACACGACTAGCTAAAATCGAGTAAAACTTTTCTATCTCTCGGGGCTTCATTTTCTTGTTCTCACATCGTCATAATTCATGTTTGTTTCTCATTTCTGAGATGTACAAGCTAGACGTATGATATCGTAGGCTAGCGCTTGGGTATTTCAGTTCTAAAGTTGAGATTGGTGTTTGGAAAATTTTCCAGATT

mRNA sequence

ATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCCTTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGGTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAATCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGAATTATCAGAAATTCCAGAAATGAAACTTGAAGAATTGAAAGGCTTATGCATGTTTGAACGAGTTCCCTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGATCATATTCTCAAGAAGATTTTGCCTCCTGAAGTGGAAGTGCCTTCATCTTTTGAGACAATACTTTTATGTCACGTTGCTCACCTGAATATACATGACGAATTGCTTCCATATAAGGATGTCATTGCGAAGGTTATTTATGATAAAAATTATCCGAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTAAAGCAATATGGGGCAACTTTTAAGCTCGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGCTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCGTCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCGTTCATGTATATAATTTGGATGCTAGGAAGTTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAATCTGTCACCTCAATACTAAAAGCTTGTGAGAAGGGCATTGGAGGGACCAATCAAGAATCACCATCGGCAAATGTTCAGGTGCAAGAAGTTCAAGATTATATTAAAATTAGCAATAAATCTGTAGAACACTCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCAGAAAGTTGTGAGAACGAGAAAGACAATGGAATTGCTGATGATGCTTCCACTCGTGTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCAGTGTATCTCATGCTAGGACCTGGGAACACGTTGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGATGCATTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAACCAAGATTTTATCATTTCGGAGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGCGTTGATGAAGACACGACTAGCTAA

Coding sequence (CDS)

ATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCCTTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGGTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAATCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGAATTATCAGAAATTCCAGAAATGAAACTTGAAGAATTGAAAGGCTTATGCATGTTTGAACGAGTTCCCTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGATCATATTCTCAAGAAGATTTTGCCTCCTGAAGTGGAAGTGCCTTCATCTTTTGAGACAATACTTTTATGTCACGTTGCTCACCTGAATATACATGACGAATTGCTTCCATATAAGGATGTCATTGCGAAGGTTATTTATGATAAAAATTATCCGAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTAAAGCAATATGGGGCAACTTTTAAGCTCGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGCTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCGTCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCGTTCATGTATATAATTTGGATGCTAGGAAGTTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAATCTGTCACCTCAATACTAAAAGCTTGTGAGAAGGGCATTGGAGGGACCAATCAAGAATCACCATCGGCAAATGTTCAGGTGCAAGAAGTTCAAGATTATATTAAAATTAGCAATAAATCTGTAGAACACTCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCAGAAAGTTGTGAGAACGAGAAAGACAATGGAATTGCTGATGATGCTTCCACTCGTGTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCAGTGTATCTCATGCTAGGACCTGGGAACACGTTGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGATGCATTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAACCAAGATTTTATCATTTCGGAGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGCGTTGATGAAGACACGACTAGCTAA

Homology

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BLAST of CSPI02G01360 vs. Swiss-Prot
Match: TRM52_VITVI (tRNA (guanine(37)-N1)-methyltransferase 2 OS=Vitis vinifera GN=VIT_11s0016g04350 PE=3 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 7.3e-195
Identity = 343/501 (68.46%), Postives = 403/501 (80.44%), Query Frame = 1


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           +LDESKFD+ L LWALRIP +LCK A +ILNGYL+DKPR+KP+TEDPTC+ NRY+ILSE+
Sbjct: 2   LLDESKFDLQLKLWALRIPSQLCKVASRILNGYLLDKPRVKPITEDPTCETNRYIILSER 61

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           ++ P+LS+IPE K +ELK LC  E VPYSLTLGYSYW ADH+LK+ILP  VEVPSSFETI
Sbjct: 62  IKNPDLSDIPEDKRDELKVLCKIEVVPYSLTLGYSYWSADHVLKQILPDGVEVPSSFETI 121

Query: 121 L--LCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGE 180
              LCH+AHLNI  ELLPYKDVIAKVIYDKNYPRIKTVVNKVG+ITNEFRVPKFEIL G+
Sbjct: 122 TFSLCHIAHLNITGELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNEFRVPKFEILVGK 181

Query: 181 NDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQ 240
           +DMVTEVKQY ATFKLDYSLVYWNSRLEHEH+RLVS F+PG++ICDMF+G+GPFAIPAAQ
Sbjct: 182 DDMVTEVKQYRATFKLDYSLVYWNSRLEHEHMRLVSQFRPGQIICDMFSGVGPFAIPAAQ 241

Query: 241 KECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTS 300
           K C+VYANDLNPDS+RYLK+NA+INKV   +  YN+DARKFISQLM VP +E   E    
Sbjct: 242 KGCLVYANDLNPDSIRYLKINAKINKVDDNIWAYNMDARKFISQLMEVPVHEILPEPDVP 301

Query: 301 ILKACEKGIGGTNQESPSAN----VQVQEVQDYIKISNKSVEHSRS-ADISVAVLKRSSE 360
           +LKA E+     N ++ S N    V+   V      +   +E S + AD +VA +K+ S 
Sbjct: 302 VLKATEECSIQANVQTESQNGGLTVEAIGVHSDDSSNMDGIEGSCTVADDTVAAVKKHSG 361

Query: 361 SCENEKDNGIADDASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALHFL 420
           SCE E  NG  D+A+  V GRR    NKRM+GS S S+ + WEHVDHVIMNLPASA+ FL
Sbjct: 362 SCEEE--NGTYDNATVSVVGRRKDRMNKRMRGSESSSNIKPWEHVDHVIMNLPASAIQFL 421

Query: 421 DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPN 480
           DAFRG I++KYWKGSLPWIHCYCF+RANE  + IISEAE+ALNA+IQ+PI HRVRDVAPN
Sbjct: 422 DAFRGLIQRKYWKGSLPWIHCYCFIRANETNEMIISEAETALNASIQEPILHRVRDVAPN 481

Query: 481 KAMYCLSFRLPEQ-CVDEDTT 493
           KAM+CLSFRL E+ C  ED T
Sbjct: 482 KAMFCLSFRLSEEACFKEDAT 500

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BLAST of CSPI02G01360 vs. Swiss-Prot
Match: TRM51_ARATH (tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana GN=At3g56120 PE=2 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 2.9e-175
Identity = 306/490 (62.45%), Postives = 374/490 (76.33%), Query Frame = 1


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           M DESKFDV+L LWALRIPRELCK+A +ILNGY+++ PRIKP+TEDPTC+K R VILSE 
Sbjct: 1   MFDESKFDVNLKLWALRIPRELCKSASRILNGYMLNMPRIKPITEDPTCEKTRLVILSES 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           V+  +LSEIPE KL +LK L   E VP+S+TLGYSYW ADH+LK+ILP  +++PSSFETI
Sbjct: 61  VKNADLSEIPEEKLNQLKKLSELEVVPHSVTLGYSYWSADHLLKQILPDGLDIPSSFETI 120

Query: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
              H+AHLN+HDELLP+KDVIAKVIYDKNYPRIKT+VNKVG+I+NEFRVPKFE+L GEN 
Sbjct: 121 --GHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRVPKFEVLAGENG 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           M TEVKQYGA FKLDY LVYWNSRLEHEH+RL SLF+PGE +CDMFAGIGPFAIPAAQK 
Sbjct: 181 METEVKQYGARFKLDYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFAGIGPFAIPAAQKG 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           C VYANDLNPDSVRYLK+NA+ NKV   + V+N+DARKF S LM V   E N +SV    
Sbjct: 241 CFVYANDLNPDSVRYLKINAKFNKVDDLICVHNMDARKFFSHLMAVSTCEDNLQSVADND 300

Query: 301 KACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCENEKD 360
           K  E  +    + + S         + I+ SN S+     A+      K+ S + + E  
Sbjct: 301 KTKEAAVSRGGETNSSG--------EEIRESNASINEPLGAN------KKPSGTTKTE-- 360

Query: 361 NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK 420
           NG+  D  +       G+ NKR++ + +  A+ WEH+DHVIMNLPASAL FLD+F   I+
Sbjct: 361 NGVGKDCKS-----IEGHANKRLRQTLLPIAKPWEHIDHVIMNLPASALQFLDSFSNVIQ 420

Query: 421 KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF 480
           KKYWKG LP IHCYCF+RA+E  +FII+EAE+AL  +I+DP+FH+VRDVAPNKAM+CLSF
Sbjct: 421 KKYWKGPLPLIHCYCFIRASETTEFIIAEAETALKFHIEDPVFHKVRDVAPNKAMFCLSF 467

Query: 481 RLPEQCVDED 491
           RLPE C+ ++
Sbjct: 481 RLPEACLKQE 467

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BLAST of CSPI02G01360 vs. Swiss-Prot
Match: TRM5_SORBI (tRNA (guanine(37)-N1)-methyltransferase OS=Sorghum bicolor GN=Sb04g025390 PE=3 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 6.1e-157
Identity = 286/490 (58.37%), Postives = 352/490 (71.84%), Query Frame = 1


Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKIL-NGYLIDKPRIKPVTEDPTCDKNRYVILSEK 61
           LDESKF+  L LWALRIPREL  A  ++L +GYL+DKPR+K V EDP  DKNR VILSEK
Sbjct: 4   LDESKFEQRLQLWALRIPRELSSAVTRLLRSGYLLDKPRVKTVVEDPEGDKNRLVILSEK 63

Query: 62  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 121
           +Q P+LS++P+ +L+ LK LC  + VPY+LTLGYSYW A H                   
Sbjct: 64  IQKPDLSDMPQQELDSLKQLCNVDVVPYTLTLGYSYWSAGH------------------- 123

Query: 122 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 181
               VAHLNI D+LLPYK+VIAKVIYDKNYPRI+TV NKVG+ITNEFRVPKFEIL G+ND
Sbjct: 124 ----VAHLNISDDLLPYKNVIAKVIYDKNYPRIQTVANKVGTITNEFRVPKFEILAGKND 183

Query: 182 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 241
           MVTEVKQYGATFKLDY LVYWNSRL+HEHIRLVSLF+ G+VICDMFAGIGPFAIPAAQK 
Sbjct: 184 MVTEVKQYGATFKLDYGLVYWNSRLDHEHIRLVSLFKKGDVICDMFAGIGPFAIPAAQKG 243

Query: 242 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 301
           C+VYANDLNPDSV YL+ NA+INKV   +  YN+DAR F+  LM VP  E+ S+   +  
Sbjct: 244 CVVYANDLNPDSVHYLRTNAKINKVDDYIFAYNMDARVFMQNLMTVPGLETGSDCQVAAD 303

Query: 302 KACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCENEKD 361
           ++  K  G    E+ S+N    +V++    S      S  A  +    +++SE CE++  
Sbjct: 304 ESYPKE-GVPANENSSSNGNHNDVREG---SQNGANESSVASTTAKKRQQTSEECESDCQ 363

Query: 362 NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK 421
           +G A         RRN   NKR++G     ++ WEH DHV+MNLPASAL FLD F G ++
Sbjct: 364 DGDASQTK-----RRN---NKRVRGPGPPPSKPWEHFDHVLMNLPASALQFLDCFDGLVQ 423

Query: 422 KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF 481
           KKYW GSLPWIHCYCF+R++E+++ I+S   + LNA I +PIFHRVRDVAPNKAM+CLSF
Sbjct: 424 KKYWTGSLPWIHCYCFIRSSESEESILS---NKLNAKIAEPIFHRVRDVAPNKAMFCLSF 455

Query: 482 RLPEQCVDED 491
           +LP +C+ ED
Sbjct: 484 KLPMECLRED 455

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BLAST of CSPI02G01360 vs. Swiss-Prot
Match: TRM5_PHYPA (tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_141055 PE=3 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 4.7e-109
Identity = 219/473 (46.30%), Postives = 293/473 (61.95%), Query Frame = 1


Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKV 61
           +D+S F V L   ALRIP++       +L+ YL+D PR+K + EDP     R ++ SE +
Sbjct: 11  IDKSAFVVRLPRVALRIPKQKSHRVASLLSKYLLDMPRVKHIVEDPQDPSMRLLLFSEAI 70

Query: 62  QTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETIL 121
             P+L  + E K+  +K +   + V + + L YSYW  +HILK+ILP   EVPSSFETI 
Sbjct: 71  AQPDLEGVSEEKILAVKEIVPLDVVQHEVVLDYSYWPVEHILKEILPAGCEVPSSFETI- 130

Query: 122 LCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDM 181
             H+AHLN+ ++LL YK +IA+VI DKN P++KTVVNKVG+ITNEFRVP+FEIL GE  +
Sbjct: 131 -GHIAHLNLREDLLTYKKIIAEVILDKN-PKLKTVVNKVGTITNEFRVPEFEILAGEPSL 190

Query: 182 VTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKEC 241
           VTE+KQ+GATF+LDY +VYWNSRLE EH RL + F+PG+VI DMFAGIGPFAIPAAQ+ C
Sbjct: 191 VTEIKQHGATFRLDYGMVYWNSRLEGEHKRLFAQFKPGQVIVDMFAGIGPFAIPAAQQGC 250

Query: 242 IVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILK 301
            VYANDLNP SV++LK+N++INKV   +  +N            +   E   + VT    
Sbjct: 251 AVYANDLNPTSVKFLKLNSDINKVGESIKAFN------------LDAREFMRKLVTE--- 310

Query: 302 ACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCENEKDN 361
             E+G  G             E+     I+  S+  +R     +   K +S S     D 
Sbjct: 311 --EEGCVG-------------EIASSTLIAENSLNGTR-----IPSSKENSSSLTEGSDM 370

Query: 362 GIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKK 421
             +     R A +   +   +MK   +S  + WEH DHV+MNLPASAL FLD   G + K
Sbjct: 371 PSSKKPEERKAKKAKVSEVVKMK---ISDVKPWEHFDHVVMNLPASALEFLDVLNGLLCK 430

Query: 422 KYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKA 475
             WKG++P +HCYCFMR+NE    I+ +AE+ L  +I DP  + VRDVAPNKA
Sbjct: 431 GRWKGTMPRVHCYCFMRSNETNADIVKKAETYLGGSIADPDVYVVRDVAPNKA 442

[Close]

BLAST of CSPI02G01360 vs. Swiss-Prot
Match: TRM5_BATDJ (tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strain JAM81 / FGSC 10211) GN=TRM5 PE=3 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 4.3e-62
Identity = 119/282 (42.20%), Postives = 184/282 (65.25%), Query Frame = 1


Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKV 61
           L+ + F+    L AL+IP      A++ L  +++  PR++ + +DPT  K R ++L   +
Sbjct: 16  LNHALFESSHTLPALKIPAHASGIAMETLRSHILVAPRLRTIVDDPTDKKWRLLLLDPSM 75

Query: 62  QTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETIL 121
           +  E++++P   L+E       + V +++ L Y YW +D +L+ ILP E+E P +FET+ 
Sbjct: 76  EASEINDLPN-PLKEFALKHEAKLVKHTIELKYDYWTSDQVLRSILPDEMETPGAFETV- 135

Query: 122 LCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDM 181
             H+AHLN+ D   P+K +I +VI DK+   IKTVVNK+ +I + FR  + EIL G NDM
Sbjct: 136 -GHIAHLNLRDRYQPFKHIIGQVILDKS-SHIKTVVNKLDNIDHTFRFFQMEILAGINDM 195

Query: 182 VTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQ-KE 241
             ++K+ G  F  D+S VYWNSRL+ EH R++ LF   ++ICD+FAG+GPFA+PAA+ K 
Sbjct: 196 NAKLKEGGCFFHFDFSKVYWNSRLQGEHDRIIKLFGQNDLICDVFAGVGPFALPAAKHKR 255

Query: 242 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQ 283
           C+V+ANDLNP S +YL  N ++NK+  R+  +N+D R+FI Q
Sbjct: 256 CVVFANDLNPQSFKYLMENIKLNKLETRILPFNMDGRQFIKQ 293

[Close]

BLAST of CSPI02G01360 vs. TrEMBL
Match: A0A0A0LFW8_CUCSA (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis sativus GN=Csa_2G005350 PE=3 SV=1)

HSP 1 Score: 996.5 bits (2575), Expect = 1.1e-287
Identity = 490/493 (99.39%), Postives = 491/493 (99.59%), Query Frame = 1


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
           LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND
Sbjct: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE
Sbjct: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           CIVYANDLNPDS+RYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL
Sbjct: 241 CIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300

Query: 301 KACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCENEKD 360
           KACEKGIGGTNQESP ANVQVQEVQDYIKISNKSVE SRSADISVAVLKRSSESCENEKD
Sbjct: 301 KACEKGIGGTNQESPLANVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENEKD 360

Query: 361 NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK 420
           NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK
Sbjct: 361 NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK 420

Query: 421 KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF 480
           KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF
Sbjct: 421 KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF 480

Query: 481 RLPEQCVDEDTTS 494
           RLPEQCVDEDTTS
Sbjct: 481 RLPEQCVDEDTTS 493

[Close]

BLAST of CSPI02G01360 vs. TrEMBL
Match: W9QGL8_9ROSA (tRNA (guanine(37)-N1)-methyltransferase OS=Morus notabilis GN=L484_020857 PE=3 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 6.5e-198
Identity = 350/495 (70.71%), Postives = 404/495 (81.62%), Query Frame = 1


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           M DE KFDVHL L ALRIPRELCK A KI+NGYL+DKPR+KP+TEDP C+KNRY+ILSE+
Sbjct: 1   MFDERKFDVHLKLLALRIPRELCKVATKIVNGYLLDKPRVKPITEDPACEKNRYMILSER 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQ  +LS+IP   + EL GLC  + VPYSLTLGYSYWGADHILK+ILPP VEVPSSFETI
Sbjct: 61  VQNSDLSDIPNDTINELSGLCKIDVVPYSLTLGYSYWGADHILKQILPPGVEVPSSFETI 120

Query: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
              H+AHLNIH ELLP+KDVIAKVIYDKNYPRIKTVVNKVG+ITNEFRVPKFE+L GEN+
Sbjct: 121 --GHIAHLNIHGELLPFKDVIAKVIYDKNYPRIKTVVNKVGTITNEFRVPKFEVLAGENN 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           MVTEVKQYG+TFKLDY LVYWNSRLEHEHIRLVS FQ GEVICDMFAGIGPFAIPAAQK 
Sbjct: 181 MVTEVKQYGSTFKLDYGLVYWNSRLEHEHIRLVSQFQAGEVICDMFAGIGPFAIPAAQKG 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           CIVYANDLNPDS+RYLK+NAE+NKV G V  YN DAR+FISQ+M VP  E      T+IL
Sbjct: 241 CIVYANDLNPDSIRYLKINAEVNKVDGYVCAYNTDAREFISQMMTVPKLEIELNDNTAIL 300

Query: 301 KACEKGIGG-TNQESPSANVQVQEVQDYIKISNKSVEHSR-SADISVAVLKRSSESCENE 360
           K+CE+     TNQ+  SA V+V+E  + +    +++E S  S D SVA +KR S+ C+ E
Sbjct: 301 KSCEQQQDKITNQDMESA-VEVKEPPNGVVNKVENMEGSGISGDASVASVKRPSDDCQAE 360

Query: 361 KDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGS 420
             NG A   +T  A RR GN NK+M+GS ++  +TWEHVDH+IMNLPASAL FLDAFRG 
Sbjct: 361 --NGSA-RGNTSPASRRKGNKNKKMRGSEIAATKTWEHVDHIIMNLPASALQFLDAFRGL 420

Query: 421 IKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCL 480
           I++KYWKG LPWIHCYCFMRA+E Q+ I SEAE+AL A+IQ PIFHRVRDVAPNKAM+CL
Sbjct: 421 IQRKYWKGDLPWIHCYCFMRAHETQEHIFSEAEAALGASIQKPIFHRVRDVAPNKAMFCL 480

Query: 481 SFRLPEQCVDEDTTS 494
           SFRLPE C  E+ ++
Sbjct: 481 SFRLPESCCHENVSN 489

[Close]

BLAST of CSPI02G01360 vs. TrEMBL
Match: U5FHF9_POPTR (tRNA (guanine(37)-N1)-methyltransferase OS=Populus trichocarpa GN=POPTR_0018s06240g PE=3 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 1.9e-194
Identity = 345/498 (69.28%), Postives = 398/498 (79.92%), Query Frame = 1


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDV L LWALRIP +LCKAA  ILNGYL+DKPRIKP+TEDPTC KNRYVILS++
Sbjct: 1   MLDESKFDVDLKLWALRIPCQLCKAATSILNGYLLDKPRIKPITEDPTCQKNRYVILSDR 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQ+ +LSEI   KL+ELK LC  + +PYSLTLGYSYW ADH+LK+ILPP VEVPSSFETI
Sbjct: 61  VQSHDLSEISPQKLDELKKLCEIQVIPYSLTLGYSYWSADHVLKQILPPGVEVPSSFETI 120

Query: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
               +AHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVG+ITNEFRVPKFEIL GE+D
Sbjct: 121 --GQIAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNEFRVPKFEILAGESD 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVS FQ GE+ICDMFAGIGPFAIPAAQK 
Sbjct: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSQFQRGEIICDMFAGIGPFAIPAAQKG 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           CIVYANDLNPDS+RYLK+NAEINKV   +  +N+DARKFISQLM  P  E+N+E   S+L
Sbjct: 241 CIVYANDLNPDSIRYLKINAEINKVDDCICAFNMDARKFISQLMEAPVCENNAECNVSML 300

Query: 301 KACEKGIGGTNQESPSAN----VQVQEVQDYIKISNKSVEHS-RSADISVAVLKRSSESC 360
           KACE      N+ES   N    V+ +E    +  + +  + S R+   SVA  KR S+ C
Sbjct: 301 KACEDCSVQENEESRVENTRLGVEAEEAPVTVPGNVEGAQDSNRNIHTSVAATKRPSDCC 360

Query: 361 ENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAF 420
             E  NG  +      A RR G   KRM+   + + + WEHVDH++MNLPASALHFLDAF
Sbjct: 361 LEE--NGSINGDGAMDAFRRKGGKKKRMRALELPNTKPWEHVDHIVMNLPASALHFLDAF 420

Query: 421 RGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAM 480
           +G I++K WKG+LP IHCYCFMR+N+ Q+ I+SEAESALNA+IQ+PIFHRVRDVAPNKAM
Sbjct: 421 KGRIQRKDWKGTLPLIHCYCFMRSNQTQELIVSEAESALNAHIQEPIFHRVRDVAPNKAM 480

Query: 481 YCLSFRLPEQCVDEDTTS 494
           +CLSFRLPE C  +D T+
Sbjct: 481 FCLSFRLPEACFKDDATN 494

[Close]

BLAST of CSPI02G01360 vs. TrEMBL
Match: A0A061GMW4_THECC (tRNA (guanine(37)-N1)-methyltransferase OS=Theobroma cacao GN=TCM_037932 PE=3 SV=1)

HSP 1 Score: 684.5 bits (1765), Expect = 9.6e-194
Identity = 345/501 (68.86%), Postives = 396/501 (79.04%), Query Frame = 1


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDV+  L ALRIPRE CK A ++LNGYL+DKPR+KP+TEDPT +KNRY+ILSE+
Sbjct: 1   MLDESKFDVNFQLLALRIPREFCKIATRLLNGYLLDKPRVKPITEDPTSEKNRYMILSEQ 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQ P+LS+IP  KL+EL+ LC  E VPYSLTLGYSYW ADHILK+ILPP VEVPSSFETI
Sbjct: 61  VQNPDLSDIPSQKLDELRKLCEVEVVPYSLTLGYSYWTADHILKQILPPGVEVPSSFETI 120

Query: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
              HVAHLNIHDELLP+KDVIAKVIYDKNYPRIKTVVNKVG+ITNEFRVPKFEIL GE+D
Sbjct: 121 --GHVAHLNIHDELLPFKDVIAKVIYDKNYPRIKTVVNKVGTITNEFRVPKFEILAGESD 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           MVTEVKQYGATFKLDY LVYWNSRLEHEHIRLVSLF+PGE ICDMFAGIGPFAIPAAQK 
Sbjct: 181 MVTEVKQYGATFKLDYCLVYWNSRLEHEHIRLVSLFRPGETICDMFAGIGPFAIPAAQKG 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           C+VYANDLNPDS+ YLK+NA++NKV   V  YN+DARKF+SQLM VP +E N ES  S++
Sbjct: 241 CLVYANDLNPDSIHYLKINAKLNKVDDCVFAYNMDARKFVSQLMAVPVSEVNLESNDSMI 300

Query: 301 KACE----KGIGGTNQESPSANVQVQEV-----QDYIKISNKSVEHSRSADISVAVLKRS 360
           KAC+    KGI     E     V+ +EV     +D ++    S   SR+   SV  +KR 
Sbjct: 301 KACDTFSMKGIHDAEAEERKLGVEEKEVAGNDAKDELEGMKNS---SRNVYASVVAIKRP 360

Query: 361 SESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHF 420
           S+    E  N        +   ++ GN  KRMKGS +S A+ WEHVDHVIMNLPASAL F
Sbjct: 361 SDCFTEENGN-------AQSVNKKKGNTQKRMKGSVLSKAKAWEHVDHVIMNLPASALQF 420

Query: 421 LDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAP 480
           LDAFRG   +K+WKG LP +HCYCF+RA E ++FIISEAE+ALNA IQDP+FHRVRDVAP
Sbjct: 421 LDAFRGIFSRKHWKGPLPLVHCYCFIRATETKEFIISEAETALNAKIQDPLFHRVRDVAP 480

Query: 481 NKAMYCLSFRLPEQCVDEDTT 493
           NKAM+CLSFRLPE C  ED T
Sbjct: 481 NKAMFCLSFRLPEACFIEDIT 489

[Close]

BLAST of CSPI02G01360 vs. TrEMBL
Match: K4BWE7_SOLLC (tRNA (guanine(37)-N1)-methyltransferase OS=Solanum lycopersicum PE=3 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 1.7e-190
Identity = 337/491 (68.64%), Postives = 396/491 (80.65%), Query Frame = 1


Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKV 61
           LDESKFD+HL LWALRIPRE CKAA+KILNG+L D+PR+KP+TEDPT +K RY+I SEKV
Sbjct: 4   LDESKFDMHLKLWALRIPREHCKAAMKILNGHLFDRPRVKPITEDPTNEKTRYMIFSEKV 63

Query: 62  QTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI- 121
           +TP+LS+IP  KL ELKGLC FE  PYSL+LGYSYW ADHILK+ILP  ++VPSSFETI 
Sbjct: 64  KTPDLSDIPAQKLAELKGLCQFEVAPYSLSLGYSYWSADHILKQILPSGLDVPSSFETIR 123

Query: 122 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 181
           +LCH+AHLNI D+LLPYKDVIAKVIYDKNYPRI+TVVNKVG+ITNEFRVPKFEIL G++D
Sbjct: 124 ILCHIAHLNITDDLLPYKDVIAKVIYDKNYPRIQTVVNKVGTITNEFRVPKFEILAGKDD 183

Query: 182 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 241
           MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVS FQ GE ICDMFAGIGPFAIPAAQK 
Sbjct: 184 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSKFQAGETICDMFAGIGPFAIPAAQKG 243

Query: 242 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 301
           C VYANDLNPDS+ YLK NAEI+KV   V  YN+DARKFISQL+ VP N SN+E+    +
Sbjct: 244 CAVYANDLNPDSIHYLKTNAEISKVDHLVFPYNMDARKFISQLVAVPCNGSNAEAE---I 303

Query: 302 KACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSR-SADISVAVLKRSSESCENEK 361
              +K  G  ++E  S +  V+EV D    +   +  S    D++V  +KR +ES + E 
Sbjct: 304 DCSDKFSGEAHREKLSEDYDVKEVPDGTSCNTAGLGGSSVIEDMNVTSVKRRAESSQEEG 363

Query: 362 DNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSI 421
           +   A +    VAGRR    NKR+KGS   + +  EHVDHVIMNLPASAL FLDAFRG I
Sbjct: 364 ERRSAVNDGVCVAGRRKRGPNKRVKGSESLNTKPSEHVDHVIMNLPASALEFLDAFRGLI 423

Query: 422 KKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLS 481
           +++YWKGSLPWIHCYCF+RA+E +D+IIS AESAL A +Q+P+FHRVRDVAPNKAM+CLS
Sbjct: 424 RRRYWKGSLPWIHCYCFIRASETEDYIISVAESALGAKVQNPVFHRVRDVAPNKAMFCLS 483

Query: 482 FRLPEQ-CVDE 490
           FRLPE+ C D+
Sbjct: 484 FRLPEETCTDD 491

[Close]

BLAST of CSPI02G01360 vs. TAIR10
Match: AT3G56120.1 (AT3G56120.1 S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 616.3 bits (1588), Expect = 1.6e-176
Identity = 306/490 (62.45%), Postives = 374/490 (76.33%), Query Frame = 1


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           M DESKFDV+L LWALRIPRELCK+A +ILNGY+++ PRIKP+TEDPTC+K R VILSE 
Sbjct: 1   MFDESKFDVNLKLWALRIPRELCKSASRILNGYMLNMPRIKPITEDPTCEKTRLVILSES 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           V+  +LSEIPE KL +LK L   E VP+S+TLGYSYW ADH+LK+ILP  +++PSSFETI
Sbjct: 61  VKNADLSEIPEEKLNQLKKLSELEVVPHSVTLGYSYWSADHLLKQILPDGLDIPSSFETI 120

Query: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
              H+AHLN+HDELLP+KDVIAKVIYDKNYPRIKT+VNKVG+I+NEFRVPKFE+L GEN 
Sbjct: 121 --GHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRVPKFEVLAGENG 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           M TEVKQYGA FKLDY LVYWNSRLEHEH+RL SLF+PGE +CDMFAGIGPFAIPAAQK 
Sbjct: 181 METEVKQYGARFKLDYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFAGIGPFAIPAAQKG 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           C VYANDLNPDSVRYLK+NA+ NKV   + V+N+DARKF S LM V   E N +SV    
Sbjct: 241 CFVYANDLNPDSVRYLKINAKFNKVDDLICVHNMDARKFFSHLMAVSTCEDNLQSVADND 300

Query: 301 KACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCENEKD 360
           K  E  +    + + S         + I+ SN S+     A+      K+ S + + E  
Sbjct: 301 KTKEAAVSRGGETNSSG--------EEIRESNASINEPLGAN------KKPSGTTKTE-- 360

Query: 361 NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK 420
           NG+  D  +       G+ NKR++ + +  A+ WEH+DHVIMNLPASAL FLD+F   I+
Sbjct: 361 NGVGKDCKS-----IEGHANKRLRQTLLPIAKPWEHIDHVIMNLPASALQFLDSFSNVIQ 420

Query: 421 KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF 480
           KKYWKG LP IHCYCF+RA+E  +FII+EAE+AL  +I+DP+FH+VRDVAPNKAM+CLSF
Sbjct: 421 KKYWKGPLPLIHCYCFIRASETTEFIIAEAETALKFHIEDPVFHKVRDVAPNKAMFCLSF 467

Query: 481 RLPEQCVDED 491
           RLPE C+ ++
Sbjct: 481 RLPEACLKQE 467

[Close]

BLAST of CSPI02G01360 vs. TAIR10
Match: AT4G27340.1 (AT4G27340.1 Met-10+ like family protein)

HSP 1 Score: 203.0 bits (515), Expect = 4.3e-52
Identity = 104/255 (40.78%), Postives = 158/255 (61.96%), Query Frame = 1


Query: 53  RYVILSEKVQTPELSEIPEM------KLEELKGLCMFERVPYSLTLGYSYWGADHILKKI 112
           R ++L EK    E+ ++PE       + +       FE V   LTL Y YW    IL+ +
Sbjct: 274 RLLLLDEKYSGEEVQDLPEAIKVLFAEAKMADASLSFELVKCRLTLFYDYWPMIEILEAV 333

Query: 113 LPPEVEVPSSFETILLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNE 172
           LP  + VPS+FE  ++ H+AHLN+ DE LPYK +IAKV+ DKN P+I+TVVNK+  I N+
Sbjct: 334 LPKGMIVPSAFE--MVGHIAHLNLRDEHLPYKRLIAKVVLDKNQPKIQTVVNKIDPIHND 393

Query: 173 FRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMF 232
           FR  + E+L G + +VT V + G  F +D + VYWNS+L  E  RL+  F   +V+CD+F
Sbjct: 394 FRTMQLEVLAGNHSLVTLVVENGLRFHVDLARVYWNSKLGTERQRLLLGFDQNDVVCDVF 453

Query: 233 AGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIV 292
           AG+GP A+ AA+    VYANDLNP +V +++ N+ +NK+  R+ ++N+D R+FI  +   
Sbjct: 454 AGVGPIALAAARIVKRVYANDLNPHAVEFMEQNSVVNKLEKRIEIFNMDGRRFIKAMFSS 513

Query: 293 PPNESNSESVTSILK 302
              +  ++ V ++ K
Sbjct: 514 EKGQKVTQVVMNLPK 526

[Close]

BLAST of CSPI02G01360 vs. TAIR10
Match: AT4G04670.1 (AT4G04670.1 Met-10+ like family protein / kelch repeat-containing protein)

HSP 1 Score: 56.6 bits (135), Expect = 5.0e-08
Identity = 37/115 (32.17%), Postives = 57/115 (49.57%), Query Frame = 1


Query: 165 NEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICD 224
           N  R    EIL G+N  V   ++ G  +  D +   ++     E +R+ ++    EV+ D
Sbjct: 785 NGTRDSTLEILVGDNGWVNH-RENGILYSFDATKCMFSWGNLSEKLRMGNMACENEVVVD 844

Query: 225 MFAGIGPFAIP--AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDAR 278
           +FAGIG F +P     K  +VYA + NP ++  L+ N E N VS R  +   D R
Sbjct: 845 LFAGIGYFVLPFLVRAKAKLVYACEWNPHAIEALRRNVEANSVSERCIILEGDNR 898

[Close]

BLAST of CSPI02G01360 vs. NCBI nr
Match: gi|700205519|gb|KGN60638.1| (hypothetical protein Csa_2G005350 [Cucumis sativus])

HSP 1 Score: 996.5 bits (2575), Expect = 1.6e-287
Identity = 490/493 (99.39%), Postives = 491/493 (99.59%), Query Frame = 1


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
           LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND
Sbjct: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE
Sbjct: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           CIVYANDLNPDS+RYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL
Sbjct: 241 CIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300

Query: 301 KACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCENEKD 360
           KACEKGIGGTNQESP ANVQVQEVQDYIKISNKSVE SRSADISVAVLKRSSESCENEKD
Sbjct: 301 KACEKGIGGTNQESPLANVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENEKD 360

Query: 361 NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK 420
           NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK
Sbjct: 361 NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK 420

Query: 421 KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF 480
           KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF
Sbjct: 421 KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF 480

Query: 481 RLPEQCVDEDTTS 494
           RLPEQCVDEDTTS
Sbjct: 481 RLPEQCVDEDTTS 493

[Close]

BLAST of CSPI02G01360 vs. NCBI nr
Match: gi|778665971|ref|XP_011648665.1| (PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis sativus])

HSP 1 Score: 984.2 bits (2543), Expect = 8.5e-284
Identity = 487/493 (98.78%), Postives = 488/493 (98.99%), Query Frame = 1


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
              HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND
Sbjct: 121 --GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE
Sbjct: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           CIVYANDLNPDS+RYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL
Sbjct: 241 CIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300

Query: 301 KACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCENEKD 360
           KACEKGIGGTNQESP ANVQVQEVQDYIKISNKSVE SRSADISVAVLKRSSESCENEKD
Sbjct: 301 KACEKGIGGTNQESPLANVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENEKD 360

Query: 361 NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK 420
           NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK
Sbjct: 361 NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK 420

Query: 421 KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF 480
           KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF
Sbjct: 421 KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF 480

Query: 481 RLPEQCVDEDTTS 494
           RLPEQCVDEDTTS
Sbjct: 481 RLPEQCVDEDTTS 491

[Close]

BLAST of CSPI02G01360 vs. NCBI nr
Match: gi|659071086|ref|XP_008458074.1| (PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo])

HSP 1 Score: 952.2 bits (2460), Expect = 3.6e-274
Identity = 476/494 (96.36%), Postives = 484/494 (97.98%), Query Frame = 1


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
              HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND
Sbjct: 121 --GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE
Sbjct: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFIS+LMIVPPNESNSE+VTSIL
Sbjct: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISRLMIVPPNESNSETVTSIL 300

Query: 301 KACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCENEKD 360
           KA E+GIGGTNQESP ANVQVQEVQD I+ISNK VEHSRSADISVAVLKRSSESCENEKD
Sbjct: 301 KASEEGIGGTNQESPLANVQVQEVQD-IEISNKFVEHSRSADISVAVLKRSSESCENEKD 360

Query: 361 NGIADDASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALHFLDAFRGSI 420
           + IADDASTRVAGRRNGNGNKRMKGS +VSHARTWEH+DHVIMNLPASALHFLDAFRGSI
Sbjct: 361 DRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSI 420

Query: 421 KKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLS 480
           KKKYWKGSLPWIHCYCFMRANENQDFIIS AESALNANIQDPIFHRVRDVAPNKAMYCLS
Sbjct: 421 KKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLS 480

Query: 481 FRLPEQCVDEDTTS 494
           FRLPEQCV+EDTTS
Sbjct: 481 FRLPEQCVNEDTTS 491

[Close]

BLAST of CSPI02G01360 vs. NCBI nr
Match: gi|645274956|ref|XP_008242584.1| (PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Prunus mume])

HSP 1 Score: 727.2 bits (1876), Expect = 1.9e-206
Identity = 362/492 (73.58%), Postives = 412/492 (83.74%), Query Frame = 1


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDV L LWALRIPRELCK A +ILNGYL+DKPRIKP+TEDPTC+KNRY+ILSE+
Sbjct: 1   MLDESKFDVQLKLWALRIPRELCKVATRILNGYLLDKPRIKPITEDPTCEKNRYMILSER 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQ  +LS++PE  L+ELKGLC  E VPYSLTLGYSYWGADHILK+ILPP VEVPSSFETI
Sbjct: 61  VQDSDLSDVPEKNLDELKGLCKIEVVPYSLTLGYSYWGADHILKQILPPGVEVPSSFETI 120

Query: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
              HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKT+VNKVG+I NEFRVPKFE+L GE+D
Sbjct: 121 --GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTIVNKVGTIENEFRVPKFEVLAGEDD 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVS F+ GE+ICDMFAGIGPFAIPAAQK 
Sbjct: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSRFRAGEIICDMFAGIGPFAIPAAQKG 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           CIVYANDLNPDS+RYLK+NAE NKV  RV  YN+DARKFISQLM VP N+  S+S  S+L
Sbjct: 241 CIVYANDLNPDSIRYLKINAETNKVDNRVCAYNIDARKFISQLMAVPNNDEKSDSDISML 300

Query: 301 KACEKGIGGTNQESPSAN----VQVQEVQDYIKISNKSVEHS-RSADISVAVLKRSSESC 360
           K  EK     NQE  S N    V+V+EV D +  +  +++ S R+AD  V  +KR S+SC
Sbjct: 301 KTGEKCDIQGNQEPKSENGRLAVEVKEVPDTVISNPDALQASRRNADSLVPPVKRPSDSC 360

Query: 361 ENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAF 420
           ++E ++   D  S  VAGRR G+  KRM+G  +S  +TWEHVDHVIMNLPASAL FLDAF
Sbjct: 361 QSENES--VDGTSAFVAGRRKGSKTKRMRGPEISDVKTWEHVDHVIMNLPASALQFLDAF 420

Query: 421 RGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAM 480
           RG I++KYW GSLPWIHCYCF+RANE Q++IIS AES LNA+I+DPIFHRVRDVAPNKAM
Sbjct: 421 RGLIQRKYWTGSLPWIHCYCFIRANETQEYIISVAESGLNAHIKDPIFHRVRDVAPNKAM 480

Query: 481 YCLSFRLPEQCV 488
           +CLSFRLPE C+
Sbjct: 481 FCLSFRLPEGCL 488

[Close]

BLAST of CSPI02G01360 vs. NCBI nr
Match: gi|694417672|ref|XP_009336880.1| (PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Pyrus x bretschneideri])

HSP 1 Score: 721.1 bits (1860), Expect = 1.3e-204
Identity = 363/494 (73.48%), Postives = 409/494 (82.79%), Query Frame = 1


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKF+VHL LWALRIPRELCK A +ILNGYL+DKPRIKP+TEDPTC++NRY+ILSE+
Sbjct: 1   MLDESKFNVHLKLWALRIPRELCKVATRILNGYLLDKPRIKPITEDPTCERNRYMILSER 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQ  ELS+IPE  L ELKGLC  E VPYSLTLGYSYWGADHILK+ILPP +EVPSSFETI
Sbjct: 61  VQDSELSDIPESNLNELKGLCKIEVVPYSLTLGYSYWGADHILKQILPPGLEVPSSFETI 120

Query: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
              HVAHLNIHDELL YKDVIAKVIYDKNYPRIKT+VNKVG+I NEFRVPKFE+L GEND
Sbjct: 121 --GHVAHLNIHDELLSYKDVIAKVIYDKNYPRIKTIVNKVGTIENEFRVPKFEVLAGEND 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVS F+ GE+ICDMFAGIGPFAIPAAQK 
Sbjct: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSQFRAGEIICDMFAGIGPFAIPAAQKG 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           CIVYANDLNPDS+RYLK+NAE NKV  R+  YN+DAR+FISQLM VP  +  S+S  SIL
Sbjct: 241 CIVYANDLNPDSIRYLKINAETNKVDDRIRAYNIDARQFISQLMAVPDCDEKSDSDVSIL 300

Query: 301 KA-CEKGIGGTNQESPSAN----VQVQEVQDYIKISNKSV--EHSRSADISVAVLKRSSE 360
           K   +K     NQES S N    V+ +EV+D + ISN       SR+AD+SV  +KR S+
Sbjct: 301 KKNGDKCAVEGNQESKSENGRLAVEAKEVRDAV-ISNVGTLQGSSRNADLSVPPVKRPSD 360

Query: 361 SCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLD 420
           SC++E  NG  D  +  VAGRR G   KRM+G  +S  +TWEHVDHVIMNLPASAL FLD
Sbjct: 361 SCQSE--NGSVDGTNISVAGRRKGTKTKRMRGPEISAVKTWEHVDHVIMNLPASALQFLD 420

Query: 421 AFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNK 480
           AFRG I++KYW GSLPWIHCYCF+RA E Q++I S AESALNA I+DPIFHRVRDVAPNK
Sbjct: 421 AFRGLIQRKYWTGSLPWIHCYCFIRATETQEYIKSVAESALNATIKDPIFHRVRDVAPNK 480

Query: 481 AMYCLSFRLPEQCV 488
           AM+CLSFRLPE C+
Sbjct: 481 AMFCLSFRLPEGCL 489

The following BLAST results are available for this feature:

BLAST of CSPI02G01360 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TRM52_VITVI	7.3e-195	68.46	tRNA (guanine(37)-N1)-methyltransferase 2 OS=Vitis vinifera GN=VIT_11s0016g04350...	[more]
TRM51_ARATH	2.9e-175	62.45	tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana GN=At3g56120 P...	[more]
TRM5_SORBI	6.1e-157	58.37	tRNA (guanine(37)-N1)-methyltransferase OS=Sorghum bicolor GN=Sb04g025390 PE=3 S...	[more]
TRM5_PHYPA	4.7e-109	46.30	tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrella patens subsp. patens G...	[more]
TRM5_BATDJ	4.3e-62	42.20	tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strai...	[more]

BLAST of CSPI02G01360 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LFW8_CUCSA	1.1e-287	99.39	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis sativus GN=Csa_2G005350 PE=3 ...	[more]
W9QGL8_9ROSA	6.5e-198	70.71	tRNA (guanine(37)-N1)-methyltransferase OS=Morus notabilis GN=L484_020857 PE=3 S...	[more]
U5FHF9_POPTR	1.9e-194	69.28	tRNA (guanine(37)-N1)-methyltransferase OS=Populus trichocarpa GN=POPTR_0018s062...	[more]
A0A061GMW4_THECC	9.6e-194	68.86	tRNA (guanine(37)-N1)-methyltransferase OS=Theobroma cacao GN=TCM_037932 PE=3 SV...	[more]
K4BWE7_SOLLC	1.7e-190	68.64	tRNA (guanine(37)-N1)-methyltransferase OS=Solanum lycopersicum PE=3 SV=1	[more]

BLAST of CSPI02G01360 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G56120.1	1.6e-176	62.45	S-adenosyl-L-methionine-dependent methyltransferases superfamily pro...	[more]
AT4G27340.1	4.3e-52	40.78	Met-10+ like family protein	[more]
AT4G04670.1	5.0e-08	32.17	Met-10+ like family protein / kelch repeat-containing protein	[more]

BLAST of CSPI02G01360 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700205519\|gb\|KGN60638.1\|	1.6e-287	99.39	hypothetical protein Csa_2G005350 [Cucumis sativus]	[more]
gi\|778665971\|ref\|XP_011648665.1\|	8.5e-284	98.78	PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis sativus]	[more]
gi\|659071086\|ref\|XP_008458074.1\|	3.6e-274	96.36	PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo]	[more]
gi\|645274956\|ref\|XP_008242584.1\|	1.9e-206	73.58	PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Prunus mume]	[more]
gi\|694417672\|ref\|XP_009336880.1\|	1.3e-204	73.48	PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Pyrus x bretschneideri]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR025792	tRNA_Gua_MeTrfase_euk

Vocabulary: **Molecular Function**
Term	Definition
GO:0009019	tRNA (guanine-N1-)-methyltransferase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0030488	tRNA methylation

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0048449	floral organ formation
biological_process	GO:0016570	histone modification
biological_process	GO:0009909	regulation of flower development
biological_process	GO:0030488	tRNA methylation
biological_process	GO:0009908	flower development
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005759	mitochondrial matrix
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0052906	tRNA (guanine(37)-N(1))-methyltransferase activity
molecular_function	GO:0009019	tRNA (guanine-N1-)-methyltransferase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G01360.1	CSPI02G01360.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR025792	tRNA (guanine(37)-N(1))-methyltransferase, eukaryotic	HAMAP	MF_03152	TRM5	coord: 2..484 score: 25
None	No IPR available	GENE3D	G3DSA:3.30.300.110		coord: 112..178 score: 3.3
None	No IPR available	PANTHER	PTHR23245	UNCHARACTERIZED	coord: 394..492 score: 1.2E-182coord: 1..304 score: 1.2E
None	No IPR available	PANTHER	PTHR23245:SF36	TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE	coord: 1..304 score: 1.2E-182coord: 394..492 score: 1.2E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI02G01360	Cla016749	Watermelon (97103) v1	cpiwmB165
CSPI02G01360	Csa2G005350	Cucumber (Chinese Long) v2	cpicuB065
CSPI02G01360	MELO3C004542	Melon (DHL92) v3.5.1	cpimeB140
CSPI02G01360	ClCG11G002640	Watermelon (Charleston Gray)	cpiwcgB102
CSPI02G01360	Lsi11G002790	Bottle gourd (USVL1VR-Ls)	cpilsiB096
CSPI02G01360	MELO3C004542.2	Melon (DHL92) v3.6.1	cpimedB133
CSPI02G01360	CsaV3_2G002470	Cucumber (Chinese Long) v3	cpicucB080
CSPI02G01360	Cla97C11G209100	Watermelon (97103) v2	cpiwmbB105
CSPI02G01360	Bhi06G001644	Wax gourd	cpiwgoB131
CSPI02G01360	Cucsa.116210	Cucumber (Gy14) v1	cgycpiB152
CSPI02G01360	CmaCh12G012350	Cucurbita maxima (Rimu)	cmacpiB174
CSPI02G01360	CmoCh12G012640	Cucurbita moschata (Rifu)	cmocpiB159
CSPI02G01360	Cp4.1LG07g10020	Cucurbita pepo (Zucchini)	cpecpiB822
CSPI02G01360	CsGy2G001370	Cucumber (Gy14) v2	cgybcpiB056
CSPI02G01360	Carg26002	Silver-seed gourd	carcpiB0308

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI02G01360	Wax gourd	cpiwgoB214
CSPI02G01360	Wild cucumber (PI 183967)	cpicpiB009
CSPI02G01360	Cucumber (Gy14) v1	cgycpiB349
CSPI02G01360	Cucurbita maxima (Rimu)	cmacpiB073
CSPI02G01360	Cucurbita maxima (Rimu)	cmacpiB116
CSPI02G01360	Cucurbita maxima (Rimu)	cmacpiB800
CSPI02G01360	Cucurbita moschata (Rifu)	cmocpiB066
CSPI02G01360	Cucurbita moschata (Rifu)	cmocpiB108
CSPI02G01360	Cucurbita moschata (Rifu)	cmocpiB787
CSPI02G01360	Melon (DHL92) v3.5.1	cpimeB110
CSPI02G01360	Watermelon (Charleston Gray)	cpiwcgB151
CSPI02G01360	Watermelon (97103) v1	cpiwmB171
CSPI02G01360	Cucurbita pepo (Zucchini)	cpecpiB100
CSPI02G01360	Cucurbita pepo (Zucchini)	cpecpiB370
CSPI02G01360	Cucurbita pepo (Zucchini)	cpecpiB665
CSPI02G01360	Bottle gourd (USVL1VR-Ls)	cpilsiB130
CSPI02G01360	Melon (DHL92) v3.6.1	cpimedB108
CSPI02G01360	Silver-seed gourd	carcpiB0044
CSPI02G01360	Silver-seed gourd	carcpiB0143
CSPI02G01360	Silver-seed gourd	carcpiB0603
CSPI02G01360	Cucumber (Chinese Long) v3	cpicucB073
CSPI02G01360	Watermelon (97103) v2	cpiwmbB145