BLAST of CsaV3_1G037030 vs. NCBI nr
Match:
XP_011648520.1 (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])
HSP 1 Score: 1104.7 bits (2856), Expect = 0.0e+00
Identity = 544/544 (100.00%), Postives = 544/544 (100.00%), Query Frame = 0
Query: 1 MKSFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSF 60
MKSFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSF
Sbjct: 1 MKSFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSF 60
Query: 61 YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLS 120
YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLS
Sbjct: 61 YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLS 120
Query: 121 YVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVG 180
YVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVG
Sbjct: 121 YVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVG 180
Query: 181 IGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGI 240
IGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGI
Sbjct: 181 IGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGI 240
Query: 241 IVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ 300
IVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ
Sbjct: 241 IVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ 300
Query: 301 ESGKINPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP 360
ESGKINPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP
Sbjct: 301 ESGKINPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP 360
Query: 361 FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEI 420
FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEI
Sbjct: 361 FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEI 420
Query: 421 SESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCNY 480
SESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCNY
Sbjct: 421 SESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCNY 480
Query: 481 RDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNIK 540
RDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNIK
Sbjct: 481 RDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNIK 540
Query: 541 YSAI 545
YSAI
Sbjct: 541 YSAI 544
BLAST of CsaV3_1G037030 vs. NCBI nr
Match:
XP_008445658.1 (PREDICTED: cannabidiolic acid synthase-like 1 [Cucumis melo])
HSP 1 Score: 1036.2 bits (2678), Expect = 4.0e-299
Identity = 507/544 (93.20%), Postives = 523/544 (96.14%), Query Frame = 0
Query: 1 MKSFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSF 60
MKSF LTPFALFFIVL+F+SPSWAVSTK HEAFLQCL N SLTTNYSISNLIYTPINSSF
Sbjct: 3 MKSFLLTPFALFFIVLTFLSPSWAVSTKKHEAFLQCLFNKSLTTNYSISNLIYTPINSSF 62
Query: 61 YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLS 120
YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQA I+CSKSHALQIRIRSGGHDFEGLS
Sbjct: 63 YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQAAIICSKSHALQIRIRSGGHDFEGLS 122
Query: 121 YVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVG 180
YVSDVPFI+VDLINLRSITIDVE ENAWVQSGATLGEFYYRIGEKS+TLAFPAGSCPTVG
Sbjct: 123 YVSDVPFIVVDLINLRSITIDVEKENAWVQSGATLGEFYYRIGEKSRTLAFPAGSCPTVG 182
Query: 181 IGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGI 240
IGGHLSGGGFGWLMRKYGLAADNVIDASF+DANGKVYDRESMG+DLFWAIRGGGGGSFGI
Sbjct: 183 IGGHLSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGGSFGI 242
Query: 241 IVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ 300
IVAWKVKLVRVPATVTICGS+RSLEEEDTIKLIH+WQYITN LDKNLLLGISLTGGN TQ
Sbjct: 243 IVAWKVKLVRVPATVTICGSKRSLEEEDTIKLIHQWQYITNTLDKNLLLGISLTGGNLTQ 302
Query: 301 ESGKINPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP 360
ESGK NP ALFSSFFLGKVNELMPIL+T FPELNLSKEECSEMSWIKTVL MAGFP EP
Sbjct: 303 ESGKTNPNALFSSFFLGKVNELMPILDTKFPELNLSKEECSEMSWIKTVLVMAGFPTHEP 362
Query: 361 FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEI 420
FEVLLNRTP FGLSTKIKSDYIKKPMSEAAFKTMLKRLKA+DIEVAQIMFIPYGGRMSEI
Sbjct: 363 FEVLLNRTPLFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAEDIEVAQIMFIPYGGRMSEI 422
Query: 421 SESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCNY 480
S+SE PFPHR GNIYKLGYYVKWKDQSIDAEKRHL+WIRDIYDYMTPFVSKSPRATY NY
Sbjct: 423 SKSETPFPHRVGNIYKLGYYVKWKDQSIDAEKRHLSWIRDIYDYMTPFVSKSPRATYSNY 482
Query: 481 RDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNIK 540
RDLDIG+NNKYGKATYSHARVWGFKYFGKNFDRLVH+KT IDPNDFFRNEQSIPAL +IK
Sbjct: 483 RDLDIGINNKYGKATYSHARVWGFKYFGKNFDRLVHVKTMIDPNDFFRNEQSIPALIDIK 542
Query: 541 YSAI 545
YS I
Sbjct: 543 YSVI 546
BLAST of CsaV3_1G037030 vs. NCBI nr
Match:
XP_004135742.2 (PREDICTED: cannabidiolic acid synthase-like 2 [Cucumis sativus])
HSP 1 Score: 757.3 bits (1954), Expect = 3.6e-215
Identity = 360/530 (67.92%), Postives = 434/530 (81.89%), Query Frame = 0
Query: 11 LFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNFSIQN 70
+ +VLS +PS+ ST+ E+F+QCL + ++ I+NLIYTP NSS+ S+LNFSIQN
Sbjct: 19 IIVVVLSTATPSFC-STQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQN 78
Query: 71 LRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVPFIIV 130
LRF K PKP AIITP HVSHIQA ++CSKSH QIR RSGGHDFEGLSY+SD+PF++V
Sbjct: 79 LRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLV 138
Query: 131 DLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLSGGGF 190
DLINL+SI+IDVEN+NAWV SGATLGE YYRI EKSQTLAFPAGSCPTVG+GGHLSGGG+
Sbjct: 139 DLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGY 198
Query: 191 GWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKVKLVR 250
GWL+RKYGLAAD+VIDA VDANGKV+++ESMG+DLFWAIRGGGGGSFGI+VAWKVKLV
Sbjct: 199 GWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVE 258
Query: 251 VPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ-ESGKINPTA 310
VPA VTIC + R+LEE+ +KLIH+WQY+ KLD NLLLGISLTGG T+ E GKINP A
Sbjct: 259 VPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIA 318
Query: 311 LFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPFEVLLNRTP 370
FS FLGK E++ IL FP+L L+KE+C EMSWI++VL M F ++P EVLLNR+
Sbjct: 319 SFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKEDPLEVLLNRSR 378
Query: 371 PFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEIPFPH 430
+ +KIKSDY+K+ + A K M +RLK+QD+E++QIMF+PYGG+MSE+ +SE PF H
Sbjct: 379 LYSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSH 438
Query: 431 RAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCNYRDLDIGMNN 490
R+ Y +GY KWK+ S++AEK HLNWIR+IYDYMTPFVSKSPRA Y NYRDLDIG NN
Sbjct: 439 RSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNN 498
Query: 491 KYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNI 540
KYG+ +Y ARVWG KYFGKNFDRLVH+KTK+DP+DFFR+EQSIP L I
Sbjct: 499 KYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTLSGI 547
BLAST of CsaV3_1G037030 vs. NCBI nr
Match:
XP_022152433.1 (berberine bridge enzyme-like 18 [Momordica charantia])
HSP 1 Score: 746.1 bits (1925), Expect = 8.3e-212
Identity = 359/541 (66.36%), Postives = 433/541 (80.04%), Query Frame = 0
Query: 1 MKSFFLTPFALFFIVLSFISPS--WAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINS 60
MKSF L P A F +S S WA S HE FLQCL +S +N SIS IYTP N
Sbjct: 1 MKSFPLNPLAFAFACSIVLSSSLFWAASAHKHEGFLQCLTRHS-PSNNSISKFIYTPANF 60
Query: 61 SFYSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEG 120
S+ S+LNFSIQNLRFS +TPKPL +I PSH+SHIQA ++CSK+H LQIR RSGGHDFEG
Sbjct: 61 SYSSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEG 120
Query: 121 LSYVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPT 180
LSYVS +PFI++DLI+LRSITID +N AWVQSGATLGE YYRI +KSQTLAFPAGSCPT
Sbjct: 121 LSYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPT 180
Query: 181 VGIGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSF 240
VG+GGHLSGGG+G+L+RKYGLAAD+VIDA VDANG++ +RESM DDLFW IRGGGGGSF
Sbjct: 181 VGVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSF 240
Query: 241 GIIVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGN- 300
GI+VAWK++LV VP T+T C R+L + D +KLIH+WQY+ +KL+ NLLLGISLTGG+
Sbjct: 241 GIVVAWKLRLVPVPTTLTTCTVNRTL-DRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHI 300
Query: 301 STQESGKINPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPN 360
TQ+ GK NP A F ++FLGKV+EL+ I+NT FPEL L+KE+C E SWI++ L AGF
Sbjct: 301 LTQQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSA 360
Query: 361 QEPFEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRM 420
E E LL R P +STKIKSDY+K+P+SEA FK + +RL QDIE+AQ+MF+PYGGRM
Sbjct: 361 GESLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRM 420
Query: 421 SEISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATY 480
SE SESE P+PHRA N+YK+GY VKW++QS AEKRHLNW+R+IYDYM P+V+KSPRA Y
Sbjct: 421 SEFSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAY 480
Query: 481 CNYRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALK 539
NYRDLDIG NN+Y K Y A+VWG KYFGKNF+RLV +KT++DP+DFFR+EQSIP
Sbjct: 481 VNYRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIPTFS 539
BLAST of CsaV3_1G037030 vs. NCBI nr
Match:
XP_023520318.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 745.7 bits (1924), Expect = 1.1e-211
Identity = 361/529 (68.24%), Postives = 429/529 (81.10%), Query Frame = 0
Query: 8 PFALFFIVLSFISPSWA-VSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNF 67
P L FI++ SP WA S H+ FL CL ++S T Y IS L+YTPINSS+ SVLNF
Sbjct: 5 PLTLIFIIVLSSSPLWASPSLHIHQHFLHCLSHHSPHT-YPISKLVYTPINSSYSSVLNF 64
Query: 68 SIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVP 127
SI+NL FS QTPKPL IITPSH+SHIQA ++CSK+H LQIR RSGGHDFEGLSYV+D P
Sbjct: 65 SIRNLIFSNPQTPKPLLIITPSHISHIQAAVICSKAHDLQIRTRSGGHDFEGLSYVADQP 124
Query: 128 FIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLS 187
FIIVDLINLRSI +DV+ AWVQSGATLGE YYRIGEKS++LAFPAG CPTVG+GGH S
Sbjct: 125 FIIVDLINLRSINVDVQTNTAWVQSGATLGELYYRIGEKSRSLAFPAGICPTVGVGGHFS 184
Query: 188 GGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKV 247
GGG+G ++RKYGLAADNV+DA VDANG+V+DRESMG+DLFWAIRGGGGGSFGI+VAWKV
Sbjct: 185 GGGYGLMLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 244
Query: 248 KLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKIN 307
KLV VPA VT+C + R + E+D IKLIH+WQY+ N+LD NL LGI LTGGN + G N
Sbjct: 245 KLVVVPAVVTMCSTNRDV-EDDAIKLIHRWQYVANQLDDNLFLGILLTGGNFSTRQGITN 304
Query: 308 PTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIK-TVLTMAGFPNQEPFEVLL 367
PTA F S +LGK++EL+ IL T FPEL L+K++C E SWI+ T++ G EP E LL
Sbjct: 305 PTATFFSLYLGKLDELIAILKTTFPELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALL 364
Query: 368 NRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEI 427
NRTPP STK KSDY+K P+ EAA + + +RLKAQDIEV QI+FIPYGGRMS+ISESE
Sbjct: 365 NRTPPTIESTKFKSDYVKDPIPEAAIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESET 424
Query: 428 PFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCNYRDLDI 487
PF HRAGN+YK+GY V WK+QS+DA+ RH+NWIR++ ++MTPFVSKSPRA Y NYRDLDI
Sbjct: 425 PFSHRAGNLYKIGYAVSWKEQSLDAKNRHINWIREVNEHMTPFVSKSPRAAYVNYRDLDI 484
Query: 488 GMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIP 535
G NNKYGK +Y A VWG KYFG NF+RLV++KTK+DP+DFFR+EQSIP
Sbjct: 485 GTNNKYGKTSYEQASVWGMKYFGNNFNRLVYVKTKVDPHDFFRHEQSIP 531
BLAST of CsaV3_1G037030 vs. TAIR10
Match:
AT5G44440.1 (FAD-binding Berberine family protein)
HSP 1 Score: 513.8 bits (1322), Expect = 1.3e-145
Identity = 252/537 (46.93%), Postives = 365/537 (67.97%), Query Frame = 0
Query: 10 ALFFIVLSF---ISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNF 69
+ F +L F ISP + HE FL+C L+ + N +I+T +SSF+S+L+
Sbjct: 5 SFLFTILLFSLNISPLVSAHGSNHEDFLKC-LSYRMNDNTVEPKVIHTSKDSSFFSILDS 64
Query: 70 SIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVS-DV 129
SIQN RFS +TPKP++IITP S +Q I C++ H + +R RS GH +EGLSY++ +
Sbjct: 65 SIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNK 124
Query: 130 PFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHL 189
PF ++DL NLRSI++DV+N WVQ+GAT GE YY IG+ +++LAFPAG PTVG+GG
Sbjct: 125 PFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQF 184
Query: 190 SGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWK 249
SGGG+G L+RKYGLAADN+IDA VDA+G++ DR++MG+D FWAIRGGGG SFG+I++WK
Sbjct: 185 SGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWK 244
Query: 250 VKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKI 309
VKLV VP+T+T+ Q++ ++E +++I KWQY +K+ +L + +L + S K
Sbjct: 245 VKLVDVPSTITVFKVQKTSKKE-AVRIIKKWQYAADKVPDDLFIRTTL------ERSNKN 304
Query: 310 NPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPFEVLL 369
ALF+ ++G VN L+ ++ FPEL L KE C EMSWI++VL A FP E VL
Sbjct: 305 AVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGVLT 364
Query: 370 NRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEI 429
NR LS K K D++++P+ EAA + + +RL+A + + +I+ P+GG+MSE++E E
Sbjct: 365 NRERT-SLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYET 424
Query: 430 PFPHRAGNIYKLGYYVKWK---DQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCNYRD 489
PFPHR GN+Y++ Y W+ D++ ++L W+ +Y++MTP+VSKSPR Y N++D
Sbjct: 425 PFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKD 484
Query: 490 LDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNI 540
+D+GM K Y + WG KYF NF+RLV +KT++DP DFF +EQSIP + +
Sbjct: 485 MDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPLVNKV 532
BLAST of CsaV3_1G037030 vs. TAIR10
Match:
AT4G20820.1 (FAD-binding Berberine family protein)
HSP 1 Score: 505.8 bits (1301), Expect = 3.4e-143
Identity = 249/535 (46.54%), Postives = 359/535 (67.10%), Query Frame = 0
Query: 2 KSFFLTPFALFFIVLSFISPSWAVST--KTHEAFLQCLLNNSLTTNYSISNLIYTPINSS 61
+SFF + F +S VS +FLQC L+ L + +S +I+TP ++S
Sbjct: 4 QSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQC-LSLQLNDSNIVSKVIHTPNDTS 63
Query: 62 FYSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGL 121
F SVL SIQN RFS PKP+ I+TP S +Q+ + C++ + IR RSGGHD+EGL
Sbjct: 64 FSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGL 123
Query: 122 SYVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTV 181
SYV+ PF+I+DL NLRSIT+DV+N + WVQ+GAT+GE YY IG+K++TLAFPAG CPTV
Sbjct: 124 SYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTV 183
Query: 182 GIGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFG 241
G+GGH SGGG+G L+RK+GLAAD+VIDA VDA G++ +R MG+D FWAIRGGGG SF
Sbjct: 184 GVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFC 243
Query: 242 IIVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNST 301
++++WK+ L+ VP+TVT+ + E+ +K+IH+WQ++ +K+ +L + + L
Sbjct: 244 VVLSWKIGLINVPSTVTVFNVTK-FSEQSALKIIHRWQFVADKVSDDLFIRVML------ 303
Query: 302 QESGKINPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQE 361
+ K A F +LG V L+ ++N FPEL L +++C+EMSWI++V+ A +E
Sbjct: 304 -QRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAEL-GEE 363
Query: 362 PFEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSE 421
P VL RT L+ K KSD++++PM + A + +RL+ + E AQ++F P+GG+MSE
Sbjct: 364 PINVLTKRTRA-SLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSE 423
Query: 422 ISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCN 481
I++ E PFPHR GNIY++ Y W+ D +++++ W+ +YD M+ FV+KSPR Y N
Sbjct: 424 IADYETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYIN 483
Query: 482 YRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIP 535
RDLD+GM ++ Y + WG KYF NF+RLV +KT +DP+DFF +EQSIP
Sbjct: 484 LRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524
BLAST of CsaV3_1G037030 vs. TAIR10
Match:
AT5G44400.1 (FAD-binding Berberine family protein)
HSP 1 Score: 505.0 bits (1299), Expect = 5.8e-143
Identity = 255/542 (47.05%), Postives = 358/542 (66.05%), Query Frame = 0
Query: 8 PFALFFIVLSFISPSWAVSTKT----HEAFLQCLLNNSLTTNYSISNLIYTPIN--SSFY 67
P LF I++ + S T + + F+ C+ N+ + + + P S F
Sbjct: 6 PLPLFSILVLYFSLYTITPTSSLASLQDQFINCVQRNT-HVYFPLEKTFFAPTKNVSMFS 65
Query: 68 SVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSY 127
VL + QNLRF +K PKP I +P H SH+QA+I+CSK + +R+RSGGHD+EGLSY
Sbjct: 66 QVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSY 125
Query: 128 VS--DVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTV 187
VS D PFI++DL +R + I++++ +AWVQSGAT+GE YYRI EKS+ FPAG C ++
Sbjct: 126 VSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSL 185
Query: 188 GIGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFG 247
GIGGH++GG +G +MRKYGL ADNV+DA VDANGK+ DR +MG+D FWAIRGG GGSFG
Sbjct: 186 GIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFG 245
Query: 248 IIVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNL----LLGISLTG 307
II+AWK+KLV VP TVT+ ++L+++ K+I KWQ + +KL + L L ++ TG
Sbjct: 246 IILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTG 305
Query: 308 GNSTQESGKINPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGF 367
GN T T +++ FLG LM ++ +FPEL L+ ++C EMSW++++ ++GF
Sbjct: 306 GNKT-------VTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGF 365
Query: 368 PNQEPFEVLLNRTPPF-GLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYG 427
P P VLL PF +S K KSD++K P+ E+ + + K+L +DI + +++ PYG
Sbjct: 366 PTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYG 425
Query: 428 GRMSEISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPR 487
G M++I ES+IPFPHR G ++K+ Y W D S RH+NWIRD+Y YMTP+VS +PR
Sbjct: 426 GMMAKIPESQIPFPHRKGVLFKVQYVTSWLD-SDKRPSRHINWIRDLYSYMTPYVSSNPR 485
Query: 488 ATYCNYRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIP 537
Y NYRDLD+G N K K A+VWG YF NF+RL+ +K K+DP +FFR+EQSIP
Sbjct: 486 EAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 536
BLAST of CsaV3_1G037030 vs. TAIR10
Match:
AT5G44390.1 (FAD-binding Berberine family protein)
HSP 1 Score: 498.8 bits (1283), Expect = 4.2e-141
Identity = 251/539 (46.57%), Postives = 354/539 (65.68%), Query Frame = 0
Query: 6 LTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPIN--SSFYSV 65
++ FAL+F SF + + ST + F++CL N+ +++ +TP S F V
Sbjct: 13 ISVFALYF---SFYTITLTSSTSLQDDFIKCLYRNT-NVRFTLDKTFFTPERNASIFTEV 72
Query: 66 LNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVS 125
L + QN R+ K PKP I P H SH+QA+++CSK + R+RSGGHD+EG+SYVS
Sbjct: 73 LESTAQNQRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVS 132
Query: 126 DV--PFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGI 185
+ PF+++DL LR I +D+++ +AWV++GAT+GE YYRI EKS+ FPAG P++GI
Sbjct: 133 QIEKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGI 192
Query: 186 GGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGII 245
GGH++GG +G LMRKYGLAADNV+DA VDANGK+ DR SMG+DLFWAIRGG GGSFGII
Sbjct: 193 GGHITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGII 252
Query: 246 VAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLT-GGNSTQ 305
++WK+KLV VP T+T+ ++ E++ + K++ KWQ I + L L L + T GN
Sbjct: 253 LSWKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTVSGNKAN 312
Query: 306 ESGKINPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQ-- 365
++ T + FLG+ LM ++ +FPEL L++++C EMSWI +++ +GFP
Sbjct: 313 KT----VTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPP 372
Query: 366 EPFEVLLNRTPPFG-LSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRM 425
P E+LL P G + K KSD+ KKP+ + M K+L +D A +++ PYGG+M
Sbjct: 373 PPIEILLQAKSPIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEED--AALVIWTPYGGKM 432
Query: 426 SEISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATY 485
+I ESEIPFPHR G + + YY W D S R WIR++Y YMTP+VS +PR Y
Sbjct: 433 DKIPESEIPFPHRNGTNFMIQYYRSWSD-SEKRPNRRTKWIRELYGYMTPYVSSNPRQAY 492
Query: 486 CNYRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
NYRDLD+G N K+ + A++WG YF NF+RLV +K+K+DP++FFR+EQSIP L
Sbjct: 493 VNYRDLDLGQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTL 540
BLAST of CsaV3_1G037030 vs. TAIR10
Match:
AT5G44410.1 (FAD-binding Berberine family protein)
HSP 1 Score: 496.5 bits (1277), Expect = 2.1e-140
Identity = 253/543 (46.59%), Postives = 363/543 (66.85%), Query Frame = 0
Query: 5 FLTPFALFFIVLSFI---SPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFY 64
FL ++F++L+ PS + HE FL+CL S N S +I+T + S++
Sbjct: 6 FLLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCL---SHRINEDDSRIIHTSKDPSYF 65
Query: 65 SVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSY 124
S+LN SIQN RF +TPKP++IITP + +Q+TI C++ H + IR RSGGHD+EGLSY
Sbjct: 66 SILNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSY 125
Query: 125 VS-DVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVG 184
++ PF+++DL NLRSIT+DV+N WVQSGAT+GE YY IG+ S++LAFPAG PTVG
Sbjct: 126 MAKSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVG 185
Query: 185 IGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGI 244
IGG GGG+G LMRKYGL+ADNVIDA VDANG DR+ MG+D FWAIRGGGG SF +
Sbjct: 186 IGGQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSV 245
Query: 245 IVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ 304
+++WK++L+ VP+ VT+ ++ E+E + +I+KWQYI +K+ +L + L Q
Sbjct: 246 VLSWKIRLLDVPSVVTVFKVVKTSEKE-AVSIINKWQYIADKVPNDLFIRAML------Q 305
Query: 305 ESGKINPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP 364
+ ++ A F +LG V++L+ ++ FPEL L C EMSWI++VL F E
Sbjct: 306 KETEV--YASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVL---WFIKGES 365
Query: 365 FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEI 424
E+L R S K K D+I++P+ + A + + +R +A + +A+I+ P+GG+MSEI
Sbjct: 366 MEILAKRKRT-SRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEI 425
Query: 425 SESEIPFPHRAGNIYKLGYYVKW---KDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATY 484
+++EIPFPHR GN+Y++ Y W +D++ +++L W+ +Y++MTP+VSKSPR Y
Sbjct: 426 ADNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAY 485
Query: 485 CNYRDLDIGMNNKYG-KATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 540
N+RD+D+GM K Y A+VWG KYF NFDRLV +KT +DP DFF +EQSIP +
Sbjct: 486 VNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPIM 532
BLAST of CsaV3_1G037030 vs. Swiss-Prot
Match:
sp|A6P6W0|CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)
HSP 1 Score: 554.3 bits (1427), Expect = 1.5e-156
Identity = 274/530 (51.70%), Postives = 369/530 (69.62%), Query Frame = 0
Query: 14 IVLSFISPSWAVS-TKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNFSIQNLR 73
I+ F+S + +S E FL+C + + TN + + L+YT + + S+LN +IQNLR
Sbjct: 14 IIFFFLSFNIQISIANPQENFLKC-FSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLR 73
Query: 74 FSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVPFIIVDL 133
F+ TPKPL IITP +VSHIQ TILCSK LQIR RSGGHD EG+SY+S VPF+IVDL
Sbjct: 74 FTSDTTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDL 133
Query: 134 INLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLSGGGFGW 193
N+ S+ IDV ++ AWV++GATLGE YY I E ++ L+FPAG CPTVG GGH SGGG+G
Sbjct: 134 RNMHSVKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGA 193
Query: 194 LMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKVKLVRVP 253
LMR YGLAADN+IDA V+ +GKV DR+SMG+DLFWAIRGGGG +FGII AWK++LV VP
Sbjct: 194 LMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVP 253
Query: 254 ATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKINPT--AL 313
+ TI ++++E + +KL++KWQ I +K LLL N T GK T +
Sbjct: 254 SMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSY 313
Query: 314 FSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPF----EVLLN 373
FSS F G V+ L+ ++N +FPEL + K +C ++SWI T++ +G N E+LL+
Sbjct: 314 FSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLD 373
Query: 374 RTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEIP 433
R+ + IK DY+KKP+ E A T+L++L +D+ V +F PYGG M EISES IP
Sbjct: 374 RSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIP 433
Query: 434 FPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCNYRDLDIG 493
FPHRAG +Y++ Y W+ Q + ++H+NWIR++Y++ TP+VS++PR Y NYRDLD+G
Sbjct: 434 FPHRAGIMYEIWYIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLG 493
Query: 494 MNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
N Y+ AR+WG KYFGKNF+RLV +KTK+DP++FFRNEQSIP L
Sbjct: 494 KTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540
BLAST of CsaV3_1G037030 vs. Swiss-Prot
Match:
sp|A6P6W1|CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)
HSP 1 Score: 553.9 bits (1426), Expect = 2.0e-156
Identity = 274/530 (51.70%), Postives = 368/530 (69.43%), Query Frame = 0
Query: 14 IVLSFISPSWAVS-TKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNFSIQNLR 73
I+ F+S + +S E FL+C L+ + TN + + L+YT + + S+LN ++QNLR
Sbjct: 14 IIFFFLSFNIQISIANPQENFLKC-LSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLR 73
Query: 74 FSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVPFIIVDL 133
F+ TPKPL I TP +VSHIQ TILCSK LQIR RSGGHD EG+SY+S VPF+IVDL
Sbjct: 74 FTSDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDL 133
Query: 134 INLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLSGGGFGW 193
N+ S+ IDV ++ AWV+SGATLGE YY I E ++ L+FPAG CPTVG GGH SGGG+G
Sbjct: 134 RNMHSVKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGA 193
Query: 194 LMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKVKLVRVP 253
LMR YGLAADN+IDA V+ +GKV DR+SMG+DLFWAIRGGGG +FGII AWK++LV VP
Sbjct: 194 LMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVP 253
Query: 254 ATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKINPT--AL 313
+ TI ++++E + +KL++KWQ I +K LLL N T GK T +
Sbjct: 254 SMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSY 313
Query: 314 FSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPF----EVLLN 373
FSS F G V+ L+ ++N +FPEL + K +C ++SWI T++ +G N E+LL+
Sbjct: 314 FSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLD 373
Query: 374 RTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEIP 433
R+ + IK DY+KKP+ E A T+L++L +D+ V +F PYGG M EISES IP
Sbjct: 374 RSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIP 433
Query: 434 FPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCNYRDLDIG 493
FPHRAG Y++ Y W+ Q + ++H+NWIR++Y++ TP+VS++PR Y NYRDLD+G
Sbjct: 434 FPHRAGITYEIWYIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLG 493
Query: 494 MNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
N Y+ AR+WG KYFGKNF+RLV +KTK+DP++FFRNEQSIP L
Sbjct: 494 KTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540
BLAST of CsaV3_1G037030 vs. Swiss-Prot
Match:
sp|A6P6V9|CBDAS_CANSA (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)
HSP 1 Score: 552.7 bits (1423), Expect = 4.4e-156
Identity = 286/541 (52.87%), Postives = 371/541 (68.58%), Query Frame = 0
Query: 3 SFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYS 62
SF+ +FF I S A E FL+C + + N + L+YT N + S
Sbjct: 7 SFWFVCKIIFFFFSFNIQTSIA---NPRENFLKC-FSQYIPNNATNLKLVYTQNNPLYMS 66
Query: 63 VLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYV 122
VLN +I NLRF+ TPKPL I+TPSHVSHIQ TILCSK LQIR RSGGHD EG+SY+
Sbjct: 67 VLNSTIHNLRFTSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYI 126
Query: 123 SDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIG 182
S VPF+IVDL N+RSI IDV ++ AWV++GATLGE YY + EK++ L+ AG CPTV G
Sbjct: 127 SQVPFVIVDLRNMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAG 186
Query: 183 GHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIV 242
GH GGG+G LMR YGLAADN+IDA V+ +GKV DR+SMG+DLFWA+RGGG SFGIIV
Sbjct: 187 GHFGGGGYGPLMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIV 246
Query: 243 AWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQES 302
AWK++LV VP + T+ ++ +E + +KL++KWQ I K DK+LLL N T
Sbjct: 247 AWKIRLVAVPKS-TMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQ 306
Query: 303 GKINPTAL---FSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQE 362
GK N TA+ FSS FLG V+ L+ ++N +FPEL + K +C ++SWI T++ +G N +
Sbjct: 307 GK-NKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYD 366
Query: 363 P----FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGG 422
E+LL+R+ + KIK DY+KKP+ E+ F +L++L +DI PYGG
Sbjct: 367 TDNFNKEILLDRSAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGG 426
Query: 423 RMSEISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRA 482
M EISES IPFPHRAG +Y+L Y W+ Q + ++HLNWIR+IY++MTP+VSK+PR
Sbjct: 427 IMDEISESAIPFPHRAGILYELWYICSWEKQ--EDNEKHLNWIRNIYNFMTPYVSKNPRL 486
Query: 483 TYCNYRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPA 537
Y NYRDLDIG+N+ Y+ AR+WG KYFGKNFDRLV +KT +DPN+FFRNEQSIP
Sbjct: 487 AYLNYRDLDIGINDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPP 539
BLAST of CsaV3_1G037030 vs. Swiss-Prot
Match:
sp|Q8GTB6|THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 PE=1 SV=1)
HSP 1 Score: 547.4 bits (1409), Expect = 1.9e-154
Identity = 277/540 (51.30%), Postives = 363/540 (67.22%), Query Frame = 0
Query: 3 SFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYS 62
SF+ +FF + I S A E FL+C + + N + L+YT + + S
Sbjct: 7 SFWFVCKIIFFFLSFHIQISIA---NPRENFLKC-FSKHIPNNVANPKLVYTQHDQLYMS 66
Query: 63 VLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYV 122
+LN +IQNLRF TPKPL I+TPS+ SHIQATILCSK LQIR RSGGHD EG+SY+
Sbjct: 67 ILNSTIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYI 126
Query: 123 SDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIG 182
S VPF++VDL N+ SI IDV ++ AWV++GATLGE YY I EK++ L+FP G CPTVG+G
Sbjct: 127 SQVPFVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVG 186
Query: 183 GHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIV 242
GH SGGG+G LMR YGLAADN+IDA V+ +GKV DR+SMG+DLFWAIRGGGG +FGII
Sbjct: 187 GHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIA 246
Query: 243 AWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQES 302
AWK+KLV VP+ TI ++++E +KL +KWQ I K DK+L+L N T
Sbjct: 247 AWKIKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNH 306
Query: 303 GKINPT--ALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP 362
GK T FSS F G V+ L+ ++N +FPEL + K +C E SWI T + +G N
Sbjct: 307 GKNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNT 366
Query: 363 F----EVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGR 422
E+LL+R+ + IK DY+KKP+ E A +L++L +D+ + PYGG
Sbjct: 367 ANFKKEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGI 426
Query: 423 MSEISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRAT 482
M EISES IPFPHRAG +Y+L Y W+ Q + ++H+NW+R +Y++ TP+VS++PR
Sbjct: 427 MEEISESAIPFPHRAGIMYELWYTASWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLA 486
Query: 483 YCNYRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
Y NYRDLD+G N Y+ AR+WG KYFGKNF+RLV +KTK+DPN+FFRNEQSIP L
Sbjct: 487 YLNYRDLDLGKTNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540
BLAST of CsaV3_1G037030 vs. Swiss-Prot
Match:
sp|Q33DQ2|THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 PE=3 SV=1)
HSP 1 Score: 542.3 bits (1396), Expect = 6.0e-153
Identity = 271/530 (51.13%), Postives = 360/530 (67.92%), Query Frame = 0
Query: 14 IVLSFISPSWAVS-TKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNFSIQNLR 73
I+ F+S + +S E FL+C + + N + IYT + + SVLN +IQNLR
Sbjct: 14 IIFFFLSFNIQISIANPQENFLKC-FSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLR 73
Query: 74 FSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVPFIIVDL 133
F+ TPKPL I+TPS+VSHIQA+ILCSK LQIR RSGGHD EGLSY+S VPF IVDL
Sbjct: 74 FTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDL 133
Query: 134 INLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLSGGGFGW 193
N+ ++ +D+ ++ AWV++GATLGE YY I E ++ +FP G CPTVG+GGH SGGG+G
Sbjct: 134 RNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGA 193
Query: 194 LMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKVKLVRVP 253
LMR YGLAADN+IDA V+ +GKV DR+SMG+DLFWAIRGGGG +FGII AWK+KLV VP
Sbjct: 194 LMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVP 253
Query: 254 ATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKINPT--AL 313
+ TI ++++E +KL +KWQ I K DK+L+L N T GK T
Sbjct: 254 SKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGY 313
Query: 314 FSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPF----EVLLN 373
FSS FLG V+ L+ ++N +FPEL + K +C E+SWI T + +G N E+LL+
Sbjct: 314 FSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLD 373
Query: 374 RTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEIP 433
R+ + IK DY+KK + E A +L++L +++ V + PYGG M EISES IP
Sbjct: 374 RSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIP 433
Query: 434 FPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCNYRDLDIG 493
FPHRAG +Y+L Y W+ Q + ++H+NW+R +Y++ TP+VS++PR Y NYRDLD+G
Sbjct: 434 FPHRAGIMYELWYTATWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLG 493
Query: 494 MNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
N Y+ AR+WG KYFGKNF+RLV +KTK DPN+FFRNEQSIP L
Sbjct: 494 KTNPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540
BLAST of CsaV3_1G037030 vs. TrEMBL
Match:
tr|A0A1S3BE36|A0A1S3BE36_CUCME (cannabidiolic acid synthase-like 1 OS=Cucumis melo OX=3656 GN=LOC103488617 PE=3 SV=1)
HSP 1 Score: 1036.2 bits (2678), Expect = 2.7e-299
Identity = 507/544 (93.20%), Postives = 523/544 (96.14%), Query Frame = 0
Query: 1 MKSFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSF 60
MKSF LTPFALFFIVL+F+SPSWAVSTK HEAFLQCL N SLTTNYSISNLIYTPINSSF
Sbjct: 3 MKSFLLTPFALFFIVLTFLSPSWAVSTKKHEAFLQCLFNKSLTTNYSISNLIYTPINSSF 62
Query: 61 YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLS 120
YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQA I+CSKSHALQIRIRSGGHDFEGLS
Sbjct: 63 YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQAAIICSKSHALQIRIRSGGHDFEGLS 122
Query: 121 YVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVG 180
YVSDVPFI+VDLINLRSITIDVE ENAWVQSGATLGEFYYRIGEKS+TLAFPAGSCPTVG
Sbjct: 123 YVSDVPFIVVDLINLRSITIDVEKENAWVQSGATLGEFYYRIGEKSRTLAFPAGSCPTVG 182
Query: 181 IGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGI 240
IGGHLSGGGFGWLMRKYGLAADNVIDASF+DANGKVYDRESMG+DLFWAIRGGGGGSFGI
Sbjct: 183 IGGHLSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGGSFGI 242
Query: 241 IVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ 300
IVAWKVKLVRVPATVTICGS+RSLEEEDTIKLIH+WQYITN LDKNLLLGISLTGGN TQ
Sbjct: 243 IVAWKVKLVRVPATVTICGSKRSLEEEDTIKLIHQWQYITNTLDKNLLLGISLTGGNLTQ 302
Query: 301 ESGKINPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP 360
ESGK NP ALFSSFFLGKVNELMPIL+T FPELNLSKEECSEMSWIKTVL MAGFP EP
Sbjct: 303 ESGKTNPNALFSSFFLGKVNELMPILDTKFPELNLSKEECSEMSWIKTVLVMAGFPTHEP 362
Query: 361 FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEI 420
FEVLLNRTP FGLSTKIKSDYIKKPMSEAAFKTMLKRLKA+DIEVAQIMFIPYGGRMSEI
Sbjct: 363 FEVLLNRTPLFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAEDIEVAQIMFIPYGGRMSEI 422
Query: 421 SESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCNY 480
S+SE PFPHR GNIYKLGYYVKWKDQSIDAEKRHL+WIRDIYDYMTPFVSKSPRATY NY
Sbjct: 423 SKSETPFPHRVGNIYKLGYYVKWKDQSIDAEKRHLSWIRDIYDYMTPFVSKSPRATYSNY 482
Query: 481 RDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNIK 540
RDLDIG+NNKYGKATYSHARVWGFKYFGKNFDRLVH+KT IDPNDFFRNEQSIPAL +IK
Sbjct: 483 RDLDIGINNKYGKATYSHARVWGFKYFGKNFDRLVHVKTMIDPNDFFRNEQSIPALIDIK 542
Query: 541 YSAI 545
YS I
Sbjct: 543 YSVI 546
BLAST of CsaV3_1G037030 vs. TrEMBL
Match:
tr|A0A1S3BEA9|A0A1S3BEA9_CUCME (cannabidiolic acid synthase-like 2 OS=Cucumis melo OX=3656 GN=LOC103488685 PE=3 SV=1)
HSP 1 Score: 741.5 bits (1913), Expect = 1.4e-210
Identity = 358/544 (65.81%), Postives = 436/544 (80.15%), Query Frame = 0
Query: 1 MKSF--FLTPFAL--FFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPI 60
MK F PF L F IV+ + + + T+ ++F+QCL + + I+N+IYTP
Sbjct: 1 MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS 60
Query: 61 NSSFYSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDF 120
NSS+ S+LNFSIQNLRF K KPLAIITPSHVSHIQA ++CSKSH QIR RSGGHDF
Sbjct: 61 NSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120
Query: 121 EGLSYVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSC 180
EGLSY+S VPF++VDLINL SI+IDVEN+NAWV+SGATLGE YYRI EKSQTLAFPAGSC
Sbjct: 121 EGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSC 180
Query: 181 PTVGIGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGG 240
PTVG+GGHLSGGG+GWL+RKYGLAAD+VIDA VDANGKV+++ESMG+DLFWAIRGGGGG
Sbjct: 181 PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG 240
Query: 241 SFGIIVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGG 300
FGI+VAWKVKLV VPA VTIC + R+LEE+ +KLIH+WQY+ KLD NLLLGISLTGG
Sbjct: 241 CFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG 300
Query: 301 NSTQ-ESGKINPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGF 360
T+ E GKINP A FS FLGK E++ IL FP+L+L+KE+C EMSWI++VL M F
Sbjct: 301 KVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWF 360
Query: 361 PNQEPFEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGG 420
+P EVLLNRT + +KIKSDY+K+ + A K M +RLK++D+E++QIMF+PYGG
Sbjct: 361 QKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGG 420
Query: 421 RMSEISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRA 480
+MSE+ +SE PF HR+ Y +GY KWK+ S++AE+RHLNWIR+IYDYMTPFVSKSPRA
Sbjct: 421 KMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRA 480
Query: 481 TYCNYRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPA 540
Y NYRDLDIG NNKYG+ +Y ARVWG KYFGKNFD+LV++KTK+DP+DFFR++QSIP
Sbjct: 481 AYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPT 540
BLAST of CsaV3_1G037030 vs. TrEMBL
Match:
tr|A0A1S3BCV7|A0A1S3BCV7_CUCME (cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488658 PE=3 SV=1)
HSP 1 Score: 721.1 bits (1860), Expect = 1.9e-204
Identity = 358/540 (66.30%), Postives = 428/540 (79.26%), Query Frame = 0
Query: 1 MKSFFLTPFALFFIVLSFISPSW--AVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINS 60
MK L P +L FIV+ S W A S HE FL CLL++S + SI+ L+YTPIN+
Sbjct: 1 MKFSLLLPLSLAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHS-PDSKSITKLVYTPINT 60
Query: 61 SFYSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEG 120
S+ VLNFSI+NLRFS TPKPL IITPS++SH+QA ++CSKSH +QIRIRSGGHDFEG
Sbjct: 61 SYSPVLNFSIRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEG 120
Query: 121 LSYVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPT 180
LSYV+ PF++VDLINL S+TI+VE AWVQSGATLGE YYRI EKS+TLAFPAG+CPT
Sbjct: 121 LSYVAYHPFVVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPT 180
Query: 181 VGIGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSF 240
VG+GGH SGGGFG L+RKYGLAADNVIDA VDANG+ +DR+SMG+DLFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSF 240
Query: 241 GIIVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNS 300
GI+VAWKVKLV VPATVTIC R+LEEE IKLI +WQY+ NKLD++L LGI+L GG
Sbjct: 241 GIVVAWKVKLVPVPATVTICTISRTLEEE-AIKLIDQWQYVANKLDEDLFLGINLLGGKI 300
Query: 301 TQESGKINPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVL-TMAGFP- 360
+ KINP ALF S FLGK +ELM ILN FP+L L+KEEC E SWI++V+ T G
Sbjct: 301 VAQGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQI 360
Query: 361 NQEPFEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGR 420
+P EVLLNRTP + K+KSDY+K+P+S+A K + +RLK+QDIE A ++FIPYGGR
Sbjct: 361 EDQPLEVLLNRTPLANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGR 420
Query: 421 MSEISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRAT 480
MS+ISESEIPF HRAGN+YK+GY W + ++AEK HLNWIRDIY YMTPFV+KSPRA
Sbjct: 421 MSQISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAA 480
Query: 481 YCNYRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
Y NYRDLDIG N+KYGK +Y A VWG KYFG NFDRLV++K ++DP +FFR+EQSIP L
Sbjct: 481 YVNYRDLDIGSNSKYGKTSYKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPVL 538
BLAST of CsaV3_1G037030 vs. TrEMBL
Match:
tr|A0A1S3BDA8|A0A1S3BDA8_CUCME (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488637 PE=3 SV=1)
HSP 1 Score: 720.7 bits (1859), Expect = 2.5e-204
Identity = 351/532 (65.98%), Postives = 429/532 (80.64%), Query Frame = 0
Query: 6 LTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLN 65
L P +L FIV+S S + S + ++ FLQCL +S + +Y IS ++YTPINSS+ SVL+
Sbjct: 7 LIPLSLVFIVVSSASLCASASPQKYQNFLQCLSEHS-SKSYPISKVVYTPINSSYSSVLD 66
Query: 66 FSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDV 125
FSI+NL FS+ +TPKPL IITPSHVSHIQA ++CSKSH LQIR RSGGHDFEGLSYV+
Sbjct: 67 FSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYR 126
Query: 126 PFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHL 185
PFI+VDLINLRS+ ++VEN AWV+SGATLGE YYRIGEKS+TLAFPAG CPTVGIGGH
Sbjct: 127 PFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGGHF 186
Query: 186 SGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWK 245
SGGG+G ++RK+GLAADNVIDA VDANGKV D+ESMG+DLFWAIRGGGGGSFGI+VAWK
Sbjct: 187 SGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFGIVVAWK 246
Query: 246 VKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKI 305
+KLV VP TVTIC + R+L EED IKLIH+WQY+ NKLD+N+ LGI LTGGN++ ++G
Sbjct: 247 IKLVPVPETVTICSTDRNL-EEDAIKLIHRWQYVGNKLDENIYLGIILTGGNASTQAGIT 306
Query: 306 NPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIK-TVLTMAGFPNQEPFEVL 365
NPTA F S FLG+ +EL+ L+T FPEL L K++C E SW++ T++ G E E L
Sbjct: 307 NPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIETLEPL 366
Query: 366 LNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESE 425
LNRT STKIKSDYI +P+ AA K + +RLK QDIE +Q++F+PYGGRMS+ISESE
Sbjct: 367 LNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQISESE 426
Query: 426 IPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCNYRDLD 485
PF HRA ++K+GY V WKDQS+ A+KRH++W+R++Y+YM PFVSKSPRA Y NYRDLD
Sbjct: 427 TPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTNYRDLD 486
Query: 486 IGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
IG NNKYGK +Y A VWG KYFG NFDRLV++KTK+DP DFFR+EQSIP L
Sbjct: 487 IGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536
BLAST of CsaV3_1G037030 vs. TrEMBL
Match:
tr|A0A1S3BDZ5|A0A1S3BDZ5_CUCME (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488631 PE=3 SV=1)
HSP 1 Score: 706.1 bits (1821), Expect = 6.3e-200
Identity = 347/533 (65.10%), Postives = 422/533 (79.17%), Query Frame = 0
Query: 6 LTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLN 65
L PF+L I++S S + S H+ FLQCL +S + Y IS +++TPINSS+ SVL+
Sbjct: 7 LIPFSLILILVSSSSSWVSASPHKHQNFLQCLSEHS-SKRYPISKVVHTPINSSYSSVLD 66
Query: 66 FSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDV 125
FSI+NL FS+ +TPKPL IITPSHVSHIQA ++CSKSH LQIR RSGGHDFEGLSYV+
Sbjct: 67 FSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYH 126
Query: 126 PFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHL 185
PFIIVDLINLRSI IDVE AWV+SGATLGE YYRIGEKS+TLAFP G CPTVG+GGH
Sbjct: 127 PFIIVDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPTVGVGGHF 186
Query: 186 SGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWK 245
SGGG+G ++RK+GLAADNVIDA VDANGKV+DR+SMG+DLFWAIRGGGGGSFGI+VAWK
Sbjct: 187 SGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFGIVVAWK 246
Query: 246 VKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGN-STQESGK 305
+KLV VPATVTIC + R+L + D IKL+H+WQY+ NKLD+NL LGI LTGGN +T + G
Sbjct: 247 IKLVSVPATVTICSTNRNL-KGDGIKLVHQWQYVANKLDENLFLGIMLTGGNITTTQEGI 306
Query: 306 INPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIK-TVLTMAGFPNQEPFEV 365
NP A F S FLGKV+ L+ L+ FPEL +KE+C E SWI+ T++ G E E
Sbjct: 307 ANPIATFFSLFLGKVDILVATLSAIFPELGFTKEDCVETSWIESTLIAATGVGTVESLEP 366
Query: 366 LLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISES 425
LLNRTP + K+KSDY K+P+S A + + +RLKA+DIE +Q++FIPYGGRMS+ISE
Sbjct: 367 LLNRTPSNLENGKMKSDYFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGRMSQISEM 426
Query: 426 EIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCNYRDL 485
E PF HRAG +YK+GY+V WK+QS+ A+K+H++WIRDIY YMTPFVSKSPR+ Y NYRDL
Sbjct: 427 ETPFSHRAGILYKIGYFVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSAYANYRDL 486
Query: 486 DIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
DIG N KYGK + A +WG KYFG NF RLV++KTK+DP DFFR+EQSIP L
Sbjct: 487 DIGANKKYGKTSVRQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_011648520.1 | 0.0e+00 | 100.00 | PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus] | [more] |
XP_008445658.1 | 4.0e-299 | 93.20 | PREDICTED: cannabidiolic acid synthase-like 1 [Cucumis melo] | [more] |
XP_004135742.2 | 3.6e-215 | 67.92 | PREDICTED: cannabidiolic acid synthase-like 2 [Cucumis sativus] | [more] |
XP_022152433.1 | 8.3e-212 | 66.36 | berberine bridge enzyme-like 18 [Momordica charantia] | [more] |
XP_023520318.1 | 1.1e-211 | 68.24 | berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo] | [more] |