CSPI01G24720 (gene) Wild cucumber (PI 183967)

BLAST of CSPI01G24720 vs. Swiss-Prot
Match: CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 1.9e-156
Identity = 274/530 (51.70%), Postives = 369/530 (69.62%), Query Frame = 1

Query: 14  IVLSFISPSWAVS-TKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNFSIQNLR 73
           I+  F+S +  +S     E FL+C  +  + TN + + L+YT  +  + S+LN +IQNLR
Sbjct: 14  IIFFFLSFNIQISIANPQENFLKCF-SQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLR 73

Query: 74  FSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVPFIIVDL 133
           F+   TPKPL IITP +VSHIQ TILCSK   LQIR RSGGHD EG+SY+S VPF+IVDL
Sbjct: 74  FTSDTTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDL 133

Query: 134 INLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLSGGGFGW 193
            N+ S+ IDV ++ AWV++GATLGE YY I E ++ L+FPAG CPTVG GGH SGGG+G 
Sbjct: 134 RNMHSVKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGA 193

Query: 194 LMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKVKLVRVP 253
           LMR YGLAADN+IDA  V+ +GKV DR+SMG+DLFWAIRGGGG +FGII AWK++LV VP
Sbjct: 194 LMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVP 253

Query: 254 ATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKTNPT--AL 313
           +  TI   ++++E  + +KL++KWQ I    +K LLL       N T   GK   T  + 
Sbjct: 254 SMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSY 313

Query: 314 FSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPF----EVLLN 373
           FSS F G V+ L+ ++N +FPEL + K +C ++SWI T++  +G  N        E+LL+
Sbjct: 314 FSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLD 373

Query: 374 RTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEIP 433
           R+     +  IK DY+KKP+ E A  T+L++L  +D+ V   +F PYGG M EISES IP
Sbjct: 374 RSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIP 433

Query: 434 FPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNYRDLDIG 493
           FPHRAG +Y++ Y   W+ Q  +  ++H+NWIR++Y++ TP+VS++PR  Y NYRDLD+G
Sbjct: 434 FPHRAGIMYEIWYIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLG 493

Query: 494 MNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
             N      Y+ AR+WG KYFGKNF+RLV +KTK+DP++FFRNEQSIP L
Sbjct: 494 KTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of CSPI01G24720 vs. Swiss-Prot
Match: CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 2.5e-156
Identity = 274/530 (51.70%), Postives = 368/530 (69.43%), Query Frame = 1

Query: 14  IVLSFISPSWAVS-TKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNFSIQNLR 73
           I+  F+S +  +S     E FL+CL +  + TN + + L+YT  +  + S+LN ++QNLR
Sbjct: 14  IIFFFLSFNIQISIANPQENFLKCL-SQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLR 73

Query: 74  FSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVPFIIVDL 133
           F+   TPKPL I TP +VSHIQ TILCSK   LQIR RSGGHD EG+SY+S VPF+IVDL
Sbjct: 74  FTSDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDL 133

Query: 134 INLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLSGGGFGW 193
            N+ S+ IDV ++ AWV+SGATLGE YY I E ++ L+FPAG CPTVG GGH SGGG+G 
Sbjct: 134 RNMHSVKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGA 193

Query: 194 LMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKVKLVRVP 253
           LMR YGLAADN+IDA  V+ +GKV DR+SMG+DLFWAIRGGGG +FGII AWK++LV VP
Sbjct: 194 LMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVP 253

Query: 254 ATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKTNPT--AL 313
           +  TI   ++++E  + +KL++KWQ I    +K LLL       N T   GK   T  + 
Sbjct: 254 SMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSY 313

Query: 314 FSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPF----EVLLN 373
           FSS F G V+ L+ ++N +FPEL + K +C ++SWI T++  +G  N        E+LL+
Sbjct: 314 FSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLD 373

Query: 374 RTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEIP 433
           R+     +  IK DY+KKP+ E A  T+L++L  +D+ V   +F PYGG M EISES IP
Sbjct: 374 RSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIP 433

Query: 434 FPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNYRDLDIG 493
           FPHRAG  Y++ Y   W+ Q  +  ++H+NWIR++Y++ TP+VS++PR  Y NYRDLD+G
Sbjct: 434 FPHRAGITYEIWYIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLG 493

Query: 494 MNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
             N      Y+ AR+WG KYFGKNF+RLV +KTK+DP++FFRNEQSIP L
Sbjct: 494 KTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of CSPI01G24720 vs. Swiss-Prot
Match: CBDAS_CANSA (Cannabidiolic acid synthase OS=Cannabis sativa GN=CBDAS PE=1 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 1.6e-155
Identity = 286/541 (52.87%), Postives = 371/541 (68.58%), Query Frame = 1

Query: 3   SFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYS 62
           SF+     +FF     I  S A      E FL+C  +  +  N +   L+YT  N  + S
Sbjct: 7   SFWFVCKIIFFFFSFNIQTSIA---NPRENFLKCF-SQYIPNNATNLKLVYTQNNPLYMS 66

Query: 63  VLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYV 122
           VLN +I NLRF+   TPKPL I+TPSHVSHIQ TILCSK   LQIR RSGGHD EG+SY+
Sbjct: 67  VLNSTIHNLRFTSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYI 126

Query: 123 SDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIG 182
           S VPF+IVDL N+RSI IDV ++ AWV++GATLGE YY + EK++ L+  AG CPTV  G
Sbjct: 127 SQVPFVIVDLRNMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAG 186

Query: 183 GHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIV 242
           GH  GGG+G LMR YGLAADN+IDA  V+ +GKV DR+SMG+DLFWA+RGGG  SFGIIV
Sbjct: 187 GHFGGGGYGPLMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIV 246

Query: 243 AWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQES 302
           AWK++LV VP + T+   ++ +E  + +KL++KWQ I  K DK+LLL       N T   
Sbjct: 247 AWKIRLVAVPKS-TMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQ 306

Query: 303 GKTNPTAL---FSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQE 362
           GK N TA+   FSS FLG V+ L+ ++N +FPEL + K +C ++SWI T++  +G  N +
Sbjct: 307 GK-NKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYD 366

Query: 363 P----FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGG 422
                 E+LL+R+     + KIK DY+KKP+ E+ F  +L++L  +DI        PYGG
Sbjct: 367 TDNFNKEILLDRSAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGG 426

Query: 423 RMSEISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRA 482
            M EISES IPFPHRAG +Y+L Y   W+ Q  +  ++HLNWIR+IY++MTP+VSK+PR 
Sbjct: 427 IMDEISESAIPFPHRAGILYELWYICSWEKQ--EDNEKHLNWIRNIYNFMTPYVSKNPRL 486

Query: 483 TYSNYRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPA 537
            Y NYRDLDIG+N+      Y+ AR+WG KYFGKNFDRLV +KT +DPN+FFRNEQSIP 
Sbjct: 487 AYLNYRDLDIGINDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPP 539

BLAST of CSPI01G24720 vs. Swiss-Prot
Match: THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 3.1e-154
Identity = 277/540 (51.30%), Postives = 363/540 (67.22%), Query Frame = 1

Query: 3   SFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYS 62
           SF+     +FF +   I  S A      E FL+C  +  +  N +   L+YT  +  + S
Sbjct: 7   SFWFVCKIIFFFLSFHIQISIA---NPRENFLKCF-SKHIPNNVANPKLVYTQHDQLYMS 66

Query: 63  VLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYV 122
           +LN +IQNLRF    TPKPL I+TPS+ SHIQATILCSK   LQIR RSGGHD EG+SY+
Sbjct: 67  ILNSTIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYI 126

Query: 123 SDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIG 182
           S VPF++VDL N+ SI IDV ++ AWV++GATLGE YY I EK++ L+FP G CPTVG+G
Sbjct: 127 SQVPFVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVG 186

Query: 183 GHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIV 242
           GH SGGG+G LMR YGLAADN+IDA  V+ +GKV DR+SMG+DLFWAIRGGGG +FGII 
Sbjct: 187 GHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIA 246

Query: 243 AWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQES 302
           AWK+KLV VP+  TI   ++++E    +KL +KWQ I  K DK+L+L       N T   
Sbjct: 247 AWKIKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNH 306

Query: 303 GKTNPT--ALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP 362
           GK   T    FSS F G V+ L+ ++N +FPEL + K +C E SWI T +  +G  N   
Sbjct: 307 GKNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNT 366

Query: 363 F----EVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGR 422
                E+LL+R+     +  IK DY+KKP+ E A   +L++L  +D+     +  PYGG 
Sbjct: 367 ANFKKEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGI 426

Query: 423 MSEISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRAT 482
           M EISES IPFPHRAG +Y+L Y   W+ Q  +  ++H+NW+R +Y++ TP+VS++PR  
Sbjct: 427 MEEISESAIPFPHRAGIMYELWYTASWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLA 486

Query: 483 YSNYRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
           Y NYRDLD+G  N      Y+ AR+WG KYFGKNF+RLV +KTK+DPN+FFRNEQSIP L
Sbjct: 487 YLNYRDLDLGKTNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of CSPI01G24720 vs. Swiss-Prot
Match: THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 7.6e-153
Identity = 271/530 (51.13%), Postives = 360/530 (67.92%), Query Frame = 1

Query: 14  IVLSFISPSWAVS-TKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNFSIQNLR 73
           I+  F+S +  +S     E FL+C  +  +  N +    IYT  +  + SVLN +IQNLR
Sbjct: 14  IIFFFLSFNIQISIANPQENFLKCF-SEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLR 73

Query: 74  FSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVPFIIVDL 133
           F+   TPKPL I+TPS+VSHIQA+ILCSK   LQIR RSGGHD EGLSY+S VPF IVDL
Sbjct: 74  FTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDL 133

Query: 134 INLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLSGGGFGW 193
            N+ ++ +D+ ++ AWV++GATLGE YY I E ++  +FP G CPTVG+GGH SGGG+G 
Sbjct: 134 RNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGA 193

Query: 194 LMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKVKLVRVP 253
           LMR YGLAADN+IDA  V+ +GKV DR+SMG+DLFWAIRGGGG +FGII AWK+KLV VP
Sbjct: 194 LMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVP 253

Query: 254 ATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKTNPT--AL 313
           +  TI   ++++E    +KL +KWQ I  K DK+L+L       N T   GK   T    
Sbjct: 254 SKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGY 313

Query: 314 FSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPF----EVLLN 373
           FSS FLG V+ L+ ++N +FPEL + K +C E+SWI T +  +G  N        E+LL+
Sbjct: 314 FSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLD 373

Query: 374 RTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEIP 433
           R+     +  IK DY+KK + E A   +L++L  +++ V   +  PYGG M EISES IP
Sbjct: 374 RSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIP 433

Query: 434 FPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNYRDLDIG 493
           FPHRAG +Y+L Y   W+ Q  +  ++H+NW+R +Y++ TP+VS++PR  Y NYRDLD+G
Sbjct: 434 FPHRAGIMYELWYTATWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLG 493

Query: 494 MNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
             N      Y+ AR+WG KYFGKNF+RLV +KTK DPN+FFRNEQSIP L
Sbjct: 494 KTNPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540

BLAST of CSPI01G24720 vs. TrEMBL
Match: B9NA97_POPTR (FAD-binding domain-containing family protein OS=Populus trichocarpa GN=POPTR_0001s43120g PE=4 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 4.9e-183
Identity = 307/530 (57.92%), Postives = 402/530 (75.85%), Query Frame = 1

Query: 11  LFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNFSIQN 70
           LFF++ SF   SW  S  THE+FLQCL + +   ++SIS LIYTPINSS+ S+L FSIQN
Sbjct: 5   LFFLIFSF---SWVTSAHTHESFLQCLDSQN---SHSISKLIYTPINSSYSSILQFSIQN 64

Query: 71  LRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVPFIIV 130
           LRF+   TPKPL I+TP+++SHIQA I+CS+ H +QIRIRSGGHD+EGLSYVS   F+++
Sbjct: 65  LRFNTSSTPKPLVIVTPTNISHIQAAIICSQKHGMQIRIRSGGHDYEGLSYVSTFSFVVI 124

Query: 131 DLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLSGGGF 190
           DLINLR+I ++ EN+ AWVQSG T+GE YYRI EKS+TLAFPA  CPTVG+GGH SGGG+
Sbjct: 125 DLINLRTINVNAENKTAWVQSGVTIGEVYYRIAEKSRTLAFPASVCPTVGVGGHFSGGGY 184

Query: 191 GWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKVKLVR 250
           G LMRKYGLAAD++IDA  VD  G++ DRESMG+DLFWAIRGGGG +FG+++AWK+ LV 
Sbjct: 185 GMLMRKYGLAADHIIDAQLVDVKGRILDRESMGEDLFWAIRGGGGNTFGVVIAWKISLVS 244

Query: 251 VPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKTNPT-- 310
            P TVT+   QR+L E++  KL+H+WQ++ +KL ++L + I L   NST+E GK+N T  
Sbjct: 245 APPTVTVFNVQRTL-EQNASKLVHRWQFVADKLHEDLFIRIILNRVNSTEE-GKSNTTIQ 304

Query: 311 ALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPFEVLLNRT 370
           A F+S FLG ++ L+P++  +FPEL L KE+C EMSWI++VL   GFP+    +VLL+RT
Sbjct: 305 ASFNSLFLGGIDRLLPLIQDSFPELGLVKEDCIEMSWIQSVLYFDGFPSNSSLDVLLDRT 364

Query: 371 PPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEIPFP 430
           P    + K KSDY+K+P+ E   + + +R   +DI    ++F PYGG+MSEISES IPFP
Sbjct: 365 PSTRRNFKAKSDYVKEPIPELGLEGIWERFFDKDINTPILIFSPYGGKMSEISESSIPFP 424

Query: 431 HRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNYRDLDIGMN 490
           HRAGNIYK+ + + W ++ I A KRH++WIR +Y Y+ P+VSK+PRA Y NYRDLDIG+N
Sbjct: 425 HRAGNIYKIQHLIYWDEEGIVATKRHISWIRRLYSYLAPYVSKTPRAAYVNYRDLDIGIN 484

Query: 491 NKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKN 539
           N  G  +Y  A +WG KYF  NFDRLV +KT +DP +FFRNEQSIP L +
Sbjct: 485 NHAGNTSYRQASIWGLKYFKNNFDRLVRVKTAVDPANFFRNEQSIPPLSS 526

BLAST of CSPI01G24720 vs. TrEMBL
Match: B9N5Q8_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s16580g PE=4 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 3.7e-178
Identity = 309/533 (57.97%), Postives = 391/533 (73.36%), Query Frame = 1

Query: 6   LTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLN 65
           + PF L F++ SF+   W+ S  THE FLQCL   S  +N S S L+YTP NSS+  VL 
Sbjct: 10  MLPF-LCFLLFSFL---WSASAHTHEDFLQCLSLYSEDSN-SFSKLVYTPNNSSYLHVLQ 69

Query: 66  FSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDV 125
            SIQN RF+   TPKP  I+TP +VSHIQATI CS+ H + +RIRSGGHD+EGLSYVS +
Sbjct: 70  GSIQNSRFNTTATPKPPVIVTPMNVSHIQATIFCSQKHGMNVRIRSGGHDYEGLSYVSVL 129

Query: 126 PFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHL 185
           PF+I+DLINLR+I +DVEN  AWVQ+GATLGE YY I EKS TLAFPAG+CPTVG GGHL
Sbjct: 130 PFVIIDLINLRAINVDVENSTAWVQAGATLGELYYSIAEKSGTLAFPAGACPTVGAGGHL 189

Query: 186 SGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWK 245
           SGGG+G LMRKYGLAADN+IDA  +DA G++ DR SMG+DLFWAIRGGGG +FG++VAWK
Sbjct: 190 SGGGYGGLMRKYGLAADNIIDAQLIDAKGRILDRASMGEDLFWAIRGGGGNTFGVVVAWK 249

Query: 246 VKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKT 305
           +KLV VP TVT     RSLEE  T KLIH+WQY+ NKL ++L +   +T  NS+QE G +
Sbjct: 250 LKLVPVPHTVTFFSVLRSLEENAT-KLIHRWQYVANKLPEDLFITAYITKINSSQE-GIS 309

Query: 306 NPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPFEVLL 365
              A F S FLG  + L+P++  NFPEL L K++C+EMSW++ VL  +G+ +    +VLL
Sbjct: 310 TIQAEFPSLFLGGADRLLPLMQENFPELGLVKDDCTEMSWVEFVLYNSGYSSNSSLDVLL 369

Query: 366 NRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEI 425
           NRTP +  + K KSDY+KKPM E AF+ + KR     IE  +++ +PYGG+M +ISES I
Sbjct: 370 NRTPQYITNFKAKSDYVKKPMPEIAFEGIWKRFLKVGIETPRLVLVPYGGKMDQISESSI 429

Query: 426 PFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNYRDLDI 485
           PF HRAGN+YK+ Y + W +Q  +A  RH+ WIR +Y Y  P+VSK+PR  Y NYRDLD+
Sbjct: 430 PFAHRAGNLYKIQYLLLWNEQGKEASMRHIAWIRRLYSYTAPYVSKNPREAYINYRDLDV 489

Query: 486 GMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKN 539
           GMNN  G  +Y  A +WG KYF  NFDRL+ +KT +DP++FFRNEQSIP L +
Sbjct: 490 GMNNIQGNTSYKQASIWGRKYFKNNFDRLIQVKTAVDPDNFFRNEQSIPPLSS 535

BLAST of CSPI01G24720 vs. TrEMBL
Match: B9GH03_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s46900g PE=4 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 4.2e-174
Identity = 301/524 (57.44%), Postives = 397/524 (75.76%), Query Frame = 1

Query: 13  FIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNFSIQNLR 72
           F+V    S S A S+ THE FL+CL +       +IS ++YT  NSS+ SVL+FSI+NLR
Sbjct: 11  FLVFLLSSSSLATSSHTHEDFLRCL-SLYFEDPVAISKVVYTSNNSSYSSVLHFSIRNLR 70

Query: 73  FSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVPFIIVDL 132
           F+   T KPL I+TP+ +SHIQATI CS+ H LQIR+RSGGHDFEGLSY S +PF++VDL
Sbjct: 71  FN-SSTLKPLVIVTPTDLSHIQATIHCSRHHNLQIRMRSGGHDFEGLSYRSVLPFVVVDL 130

Query: 133 INLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLSGGGFGW 192
           INLR+IT+D  N  AWVQ+GATLGE YYRI EKS+TLAFPAGSCPTVG+GGH SGGG+G 
Sbjct: 131 INLRTITVDATNRTAWVQAGATLGELYYRIVEKSRTLAFPAGSCPTVGVGGHFSGGGYGL 190

Query: 193 LMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKVKLVRVP 252
           L+RKYGLAADN++DA  +DA G++ DRESMGDDLFWAIRGGGG SFG++VAWK+ LV VP
Sbjct: 191 LLRKYGLAADNIVDAQLIDAKGRILDRESMGDDLFWAIRGGGGNSFGVVVAWKINLVEVP 250

Query: 253 ATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKTNPTALFS 312
           +TVT+    R+LE+  T KLIH+WQY+ NKL ++L L   +   NS+Q  G T   A+F 
Sbjct: 251 STVTVFTVVRNLEQNAT-KLIHQWQYVANKLPEDLFLAAVIRRVNSSQ-GGNTAVQAIFV 310

Query: 313 SFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPFEVLLNRTPPFG 372
           S FLGKV++L+P ++ +FP+L + +++C EMSWI+++L + GFP     +VLL+R+    
Sbjct: 311 SLFLGKVDQLLPWMHGSFPDLGIVRDDCIEMSWIESILYVYGFPRNASLDVLLDRSSQSL 370

Query: 373 LSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEIPFPHRAG 432
           ++ K+KSDY+K+PM+E   K + +R   +++EV+ + FIPYGG+M++ISES IPFPHRAG
Sbjct: 371 INFKVKSDYVKEPMAEIVLKEIWERFSDENMEVSAMSFIPYGGKMNKISESSIPFPHRAG 430

Query: 433 NIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNYRDLDIGMNNKYG 492
           N+YK+ + V W +++  A +RHL WIR +Y YMT +VS+ PR  Y NYRDLDIGMNN  G
Sbjct: 431 NLYKIIHTVAWSEET--ASERHLAWIRRLYGYMTSYVSQKPREAYINYRDLDIGMNNPVG 490

Query: 493 KATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
             +Y  A +WG KYF  NFD+LV +KT++DP +FFRNEQSIP L
Sbjct: 491 STSYGQASIWGRKYFKNNFDKLVRVKTEVDPANFFRNEQSIPPL 528

BLAST of CSPI01G24720 vs. TrEMBL
Match: U5FX93_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s16540g PE=4 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 1.4e-172
Identity = 297/529 (56.14%), Postives = 392/529 (74.10%), Query Frame = 1

Query: 11  LFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNFSIQN 70
           LF ++LSF   SW      HE FLQCL +     + +IS LIYTP + S+ SVL FS+QN
Sbjct: 14  LFLLLLSF---SWLTLAHPHEDFLQCL-SFHFQDSTAISKLIYTPRSPSYSSVLQFSVQN 73

Query: 71  LRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVS-DVPFII 130
            RF+    P+P+ I TP++VSH+QA I CS+   L IRIRSGGHD+EGLSYVS  +PF+I
Sbjct: 74  NRFNTTSDPEPVVIFTPTNVSHVQAAIYCSRKQNLHIRIRSGGHDYEGLSYVSYSLPFVI 133

Query: 131 VDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLSGGG 190
           VDLINLR + +D  ++ AWVQ+GA+LGE YYRI EK++TLAFPAG  PT+G+GGH+SGGG
Sbjct: 134 VDLINLRKVAVDARHKTAWVQAGASLGEVYYRIAEKNRTLAFPAGIWPTIGVGGHISGGG 193

Query: 191 FGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKVKLV 250
           +G +MRKYGLAADNVIDA  +D  G++ DR SMG+DLFWAIRGGGG +FG++VAWK+KLV
Sbjct: 194 YGMMMRKYGLAADNVIDAQLIDVKGRILDRASMGEDLFWAIRGGGGNTFGVVVAWKLKLV 253

Query: 251 RVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKTNPTA 310
            VP TVT+    R+LE+  T KL+H+WQ + +KL K+L + + L   NS++E GKT   A
Sbjct: 254 PVPPTVTVFTVPRTLEQNAT-KLVHRWQSVASKLHKDLTIALVLRRINSSEE-GKTTILA 313

Query: 311 LFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPFEVLLNRTP 370
            F+S FLG V+ L+P++  +FPEL L KE+C EMSWIK+VL + GFP+    +VLL RTP
Sbjct: 314 AFTSLFLGGVDRLLPLMQESFPELGLVKEDCIEMSWIKSVLYVVGFPSNASSDVLLARTP 373

Query: 371 PFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEIPFPH 430
               + K KSDY+K+PM E A + + +R    DI+  Q+++ PYGG+M EISE+ IPFPH
Sbjct: 374 LTNRNFKGKSDYVKEPMPETALEGIWERFLEADIDTPQMVWAPYGGKMDEISETSIPFPH 433

Query: 431 RAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNYRDLDIGMNN 490
           R+GN+YK+ + V W ++  +A KRH++WIR +YDY+TP+VSK+PRA Y NYRDLDIG+NN
Sbjct: 434 RSGNLYKIQHLVFWDEEGNEASKRHISWIRRLYDYLTPYVSKNPRAAYVNYRDLDIGINN 493

Query: 491 KYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKN 539
             G  +Y  A +WG KYF  NFDRLV +KT++DP +FFRNEQSIP L +
Sbjct: 494 IQGNTSYRQASIWGIKYFDNNFDRLVSVKTRVDPANFFRNEQSIPPLSS 536

BLAST of CSPI01G24720 vs. TrEMBL
Match: B9GGH9_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s46580g PE=4 SV=2)

HSP 1 Score: 613.6 bits (1581), Expect = 2.3e-172
Identity = 293/524 (55.92%), Postives = 389/524 (74.24%), Query Frame = 1

Query: 13  FIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNFSIQNLR 72
           F+ L   S SW  S   HE FLQCL       + +IS ++YTPINSS+ SVL FSI+N R
Sbjct: 11  FLFLFLFSFSWVASADRHEDFLQCL---HYQNSNAISKVVYTPINSSYSSVLQFSIRNGR 70

Query: 73  FSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVPFIIVDL 132
           F+   TPKPL I+TP +V+HIQAT+ CS+ H LQIR+RSGGHD+EGLSYV+ +PF++VDL
Sbjct: 71  FNTSATPKPLVIVTPLNVAHIQATVACSQKHGLQIRVRSGGHDYEGLSYVAVIPFVVVDL 130

Query: 133 INLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLSGGGFGW 192
           IN+R++T+DV N+ AWVQ+GATLGE YYRI EKS+TLAFPAG CPTVG GGH+SGGG G 
Sbjct: 131 INMRTVTVDVGNKIAWVQAGATLGEVYYRIAEKSRTLAFPAGVCPTVGSGGHISGGGHGM 190

Query: 193 LMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKVKLVRVP 252
           +MRKYGLAAD++IDA  +D  G++ DR SMG+DLFWAIRGGGG +FG++V WK++LV VP
Sbjct: 191 MMRKYGLAADHIIDAKLIDVKGRILDRASMGEDLFWAIRGGGGNTFGVVVEWKLELVTVP 250

Query: 253 ATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKTNPTALFS 312
            TVT+    R+LE+  T KL+H+WQ    K D++L   I L+  N++ E GKT   A+++
Sbjct: 251 PTVTVFNVTRTLEQNAT-KLVHQWQSAIGKFDEDLFSRIFLSRVNTSLE-GKTTILAVYT 310

Query: 313 SFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPFEVLLNRTPPFG 372
           S FLG V+ L+ ++  +FP+L L KE+C EMSWI++ +  A FP     +VLL+R+P + 
Sbjct: 311 SLFLGGVDRLLSMMQQSFPQLGLVKEDCIEMSWIESTVYFAQFPRNTSLDVLLDRSPGYT 370

Query: 373 LSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEIPFPHRAG 432
           +S K K+DY+K+P+ E A + + +RL   D EVAQ+ F  YGG+M EISE+ IPFPHRAG
Sbjct: 371 VSFKAKTDYVKEPLPEIALEGIWERLNQLDAEVAQLQFTAYGGKMDEISETSIPFPHRAG 430

Query: 433 NIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNYRDLDIGMNNKYG 492
           N++++ Y V W DQ  +  + + +WIR +Y YMTP+V+K+PR  Y NYRDLD+GMN+  G
Sbjct: 431 NLFQIHYGVFWGDQDSERSQIYTSWIRKLYSYMTPYVTKNPRQAYINYRDLDLGMNS-LG 490

Query: 493 KATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
             +Y  AR+WG KYF  NFDRLVH+KTK+DP +FFRNEQSIP L
Sbjct: 491 NTSYKQARIWGTKYFKNNFDRLVHVKTKVDPANFFRNEQSIPPL 528

BLAST of CSPI01G24720 vs. TAIR10
Match: AT5G44440.1 (AT5G44440.1 FAD-binding Berberine family protein)

HSP 1 Score: 513.5 bits (1321), Expect = 1.6e-145
Identity = 252/537 (46.93%), Postives = 365/537 (67.97%), Query Frame = 1

Query: 10  ALFFIVLSF---ISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNF 69
           +  F +L F   ISP  +     HE FL+CL +  +  N     +I+T  +SSF+S+L+ 
Sbjct: 5   SFLFTILLFSLNISPLVSAHGSNHEDFLKCL-SYRMNDNTVEPKVIHTSKDSSFFSILDS 64

Query: 70  SIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVS-DV 129
           SIQN RFS  +TPKP++IITP   S +Q  I C++ H + +R RS GH +EGLSY++ + 
Sbjct: 65  SIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNK 124

Query: 130 PFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHL 189
           PF ++DL NLRSI++DV+N   WVQ+GAT GE YY IG+ +++LAFPAG  PTVG+GG  
Sbjct: 125 PFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQF 184

Query: 190 SGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWK 249
           SGGG+G L+RKYGLAADN+IDA  VDA+G++ DR++MG+D FWAIRGGGG SFG+I++WK
Sbjct: 185 SGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWK 244

Query: 250 VKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKT 309
           VKLV VP+T+T+   Q++ ++E  +++I KWQY  +K+  +L +  +L      + S K 
Sbjct: 245 VKLVDVPSTITVFKVQKTSKKE-AVRIIKKWQYAADKVPDDLFIRTTL------ERSNKN 304

Query: 310 NPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPFEVLL 369
              ALF+  ++G VN L+ ++   FPEL L KE C EMSWI++VL  A FP  E   VL 
Sbjct: 305 AVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGVLT 364

Query: 370 NRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEI 429
           NR     LS K K D++++P+ EAA + + +RL+A +  + +I+  P+GG+MSE++E E 
Sbjct: 365 NRERT-SLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYET 424

Query: 430 PFPHRAGNIYKLGYYVKWK---DQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNYRD 489
           PFPHR GN+Y++ Y   W+   D++     ++L W+  +Y++MTP+VSKSPR  Y N++D
Sbjct: 425 PFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKD 484

Query: 490 LDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNI 540
           +D+GM     K  Y   + WG KYF  NF+RLV +KT++DP DFF +EQSIP +  +
Sbjct: 485 MDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPLVNKV 532

BLAST of CSPI01G24720 vs. TAIR10
Match: AT4G20820.1 (AT4G20820.1 FAD-binding Berberine family protein)

HSP 1 Score: 505.4 bits (1300), Expect = 4.5e-143
Identity = 249/535 (46.54%), Postives = 359/535 (67.10%), Query Frame = 1

Query: 2   KSFFLTPFALFFIVLSFISPSWAVSTK--THEAFLQCLLNNSLTTNYSISNLIYTPINSS 61
           +SFF +    F      +S    VS       +FLQCL +  L  +  +S +I+TP ++S
Sbjct: 4   QSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCL-SLQLNDSNIVSKVIHTPNDTS 63

Query: 62  FYSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGL 121
           F SVL  SIQN RFS    PKP+ I+TP   S +Q+ + C++   + IR RSGGHD+EGL
Sbjct: 64  FSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGL 123

Query: 122 SYVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTV 181
           SYV+  PF+I+DL NLRSIT+DV+N + WVQ+GAT+GE YY IG+K++TLAFPAG CPTV
Sbjct: 124 SYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTV 183

Query: 182 GIGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFG 241
           G+GGH SGGG+G L+RK+GLAAD+VIDA  VDA G++ +R  MG+D FWAIRGGGG SF 
Sbjct: 184 GVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFC 243

Query: 242 IIVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNST 301
           ++++WK+ L+ VP+TVT+    +   E+  +K+IH+WQ++ +K+  +L + + L      
Sbjct: 244 VVLSWKIGLINVPSTVTVFNVTK-FSEQSALKIIHRWQFVADKVSDDLFIRVML------ 303

Query: 302 QESGKTNPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQE 361
            +  K    A F   +LG V  L+ ++N  FPEL L +++C+EMSWI++V+  A    +E
Sbjct: 304 -QRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAEL-GEE 363

Query: 362 PFEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSE 421
           P  VL  RT    L+ K KSD++++PM + A   + +RL+  + E AQ++F P+GG+MSE
Sbjct: 364 PINVLTKRTRA-SLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSE 423

Query: 422 ISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSN 481
           I++ E PFPHR GNIY++ Y   W+    D +++++ W+  +YD M+ FV+KSPR  Y N
Sbjct: 424 IADYETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYIN 483

Query: 482 YRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIP 535
            RDLD+GM     ++ Y   + WG KYF  NF+RLV +KT +DP+DFF +EQSIP
Sbjct: 484 LRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of CSPI01G24720 vs. TAIR10
Match: AT5G44400.1 (AT5G44400.1 FAD-binding Berberine family protein)

HSP 1 Score: 503.4 bits (1295), Expect = 1.7e-142
Identity = 255/542 (47.05%), Postives = 358/542 (66.05%), Query Frame = 1

Query: 8   PFALFFIVLSFISPSWAVSTKT----HEAFLQCLLNNSLTTNYSISNLIYTPIN--SSFY 67
           P  LF I++ + S      T +     + F+ C+  N+    + +    + P    S F 
Sbjct: 6   PLPLFSILVLYFSLYTITPTSSLASLQDQFINCVQRNT-HVYFPLEKTFFAPTKNVSMFS 65

Query: 68  SVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSY 127
            VL  + QNLRF +K  PKP  I +P H SH+QA+I+CSK   + +R+RSGGHD+EGLSY
Sbjct: 66  QVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSY 125

Query: 128 VS--DVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTV 187
           VS  D PFI++DL  +R + I++++ +AWVQSGAT+GE YYRI EKS+   FPAG C ++
Sbjct: 126 VSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSL 185

Query: 188 GIGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFG 247
           GIGGH++GG +G +MRKYGL ADNV+DA  VDANGK+ DR +MG+D FWAIRGG GGSFG
Sbjct: 186 GIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFG 245

Query: 248 IIVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNL----LLGISLTG 307
           II+AWK+KLV VP TVT+    ++L+++   K+I KWQ + +KL + L    L  ++ TG
Sbjct: 246 IILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTG 305

Query: 308 GNSTQESGKTNPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGF 367
           GN T        T  +++ FLG    LM ++  +FPEL L+ ++C EMSW++++  ++GF
Sbjct: 306 GNKT-------VTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGF 365

Query: 368 PNQEPFEVLLNRTPPF-GLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYG 427
           P   P  VLL    PF  +S K KSD++K P+ E+  + + K+L  +DI +  +++ PYG
Sbjct: 366 PTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYG 425

Query: 428 GRMSEISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPR 487
           G M++I ES+IPFPHR G ++K+ Y   W D S     RH+NWIRD+Y YMTP+VS +PR
Sbjct: 426 GMMAKIPESQIPFPHRKGVLFKVQYVTSWLD-SDKRPSRHINWIRDLYSYMTPYVSSNPR 485

Query: 488 ATYSNYRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIP 537
             Y NYRDLD+G N K  K     A+VWG  YF  NF+RL+ +K K+DP +FFR+EQSIP
Sbjct: 486 EAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 536

BLAST of CSPI01G24720 vs. TAIR10
Match: AT5G44390.1 (AT5G44390.1 FAD-binding Berberine family protein)

HSP 1 Score: 497.7 bits (1280), Expect = 9.3e-141
Identity = 250/539 (46.38%), Postives = 355/539 (65.86%), Query Frame = 1

Query: 6   LTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSS--FYSV 65
           ++ FAL+F   SF + +   ST   + F++CL  N+    +++    +TP  ++  F  V
Sbjct: 13  ISVFALYF---SFYTITLTSSTSLQDDFIKCLYRNT-NVRFTLDKTFFTPERNASIFTEV 72

Query: 66  LNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVS 125
           L  + QN R+  K  PKP  I  P H SH+QA+++CSK   +  R+RSGGHD+EG+SYVS
Sbjct: 73  LESTAQNQRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVS 132

Query: 126 DV--PFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGI 185
            +  PF+++DL  LR I +D+++ +AWV++GAT+GE YYRI EKS+   FPAG  P++GI
Sbjct: 133 QIEKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGI 192

Query: 186 GGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGII 245
           GGH++GG +G LMRKYGLAADNV+DA  VDANGK+ DR SMG+DLFWAIRGG GGSFGII
Sbjct: 193 GGHITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGII 252

Query: 246 VAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLT-GGNSTQ 305
           ++WK+KLV VP T+T+    ++ E++ + K++ KWQ I + L   L L +  T  GN   
Sbjct: 253 LSWKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTVSGNKAN 312

Query: 306 ESGKTNPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQ-- 365
           ++     T  +   FLG+   LM ++  +FPEL L++++C EMSWI +++  +GFP    
Sbjct: 313 KT----VTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPP 372

Query: 366 EPFEVLLNRTPPFG-LSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRM 425
            P E+LL    P G +  K KSD+ KKP+     + M K+L  +D   A +++ PYGG+M
Sbjct: 373 PPIEILLQAKSPIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEED--AALVIWTPYGGKM 432

Query: 426 SEISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATY 485
            +I ESEIPFPHR G  + + YY  W D S     R   WIR++Y YMTP+VS +PR  Y
Sbjct: 433 DKIPESEIPFPHRNGTNFMIQYYRSWSD-SEKRPNRRTKWIRELYGYMTPYVSSNPRQAY 492

Query: 486 SNYRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
            NYRDLD+G N    K+ +  A++WG  YF  NF+RLV +K+K+DP++FFR+EQSIP L
Sbjct: 493 VNYRDLDLGQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTL 540

BLAST of CSPI01G24720 vs. TAIR10
Match: AT5G44410.1 (AT5G44410.1 FAD-binding Berberine family protein)

HSP 1 Score: 496.1 bits (1276), Expect = 2.7e-140
Identity = 254/543 (46.78%), Postives = 361/543 (66.48%), Query Frame = 1

Query: 4   FFLTPFALFFIVLSFIS--PSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFY 63
           F L+ F  F ++   +S  PS +     HE FL+CL   S   N   S +I+T  + S++
Sbjct: 6   FLLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCL---SHRINEDDSRIIHTSKDPSYF 65

Query: 64  SVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSY 123
           S+LN SIQN RF   +TPKP++IITP   + +Q+TI C++ H + IR RSGGHD+EGLSY
Sbjct: 66  SILNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSY 125

Query: 124 VSDV-PFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVG 183
           ++   PF+++DL NLRSIT+DV+N   WVQSGAT+GE YY IG+ S++LAFPAG  PTVG
Sbjct: 126 MAKSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVG 185

Query: 184 IGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGI 243
           IGG   GGG+G LMRKYGL+ADNVIDA  VDANG   DR+ MG+D FWAIRGGGG SF +
Sbjct: 186 IGGQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSV 245

Query: 244 IVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ 303
           +++WK++L+ VP+ VT+    ++ E+E  + +I+KWQYI +K+  +L +   L       
Sbjct: 246 VLSWKIRLLDVPSVVTVFKVVKTSEKE-AVSIINKWQYIADKVPNDLFIRAML------- 305

Query: 304 ESGKTNPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP 363
              +T   A F   +LG V++L+ ++   FPEL L    C EMSWI++VL    F   E 
Sbjct: 306 -QKETEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVL---WFIKGES 365

Query: 364 FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEI 423
            E+L  R      S K K D+I++P+ + A + + +R +A +  +A+I+  P+GG+MSEI
Sbjct: 366 MEILAKRKRT-SRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEI 425

Query: 424 SESEIPFPHRAGNIYKLGYYVKW---KDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATY 483
           +++EIPFPHR GN+Y++ Y   W   +D++    +++L W+  +Y++MTP+VSKSPR  Y
Sbjct: 426 ADNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAY 485

Query: 484 SNYRDLDIGMNNKYG-KATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 540
            N+RD+D+GM      K  Y  A+VWG KYF  NFDRLV +KT +DP DFF +EQSIP +
Sbjct: 486 VNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPIM 532

BLAST of CSPI01G24720 vs. NCBI nr
Match: gi|778665234|ref|XP_011648520.1| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 542/544 (99.63%), Postives = 542/544 (99.63%), Query Frame = 1

Query: 1   MKSFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSF 60
           MKSFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSF
Sbjct: 1   MKSFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSF 60

Query: 61  YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLS 120
           YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLS
Sbjct: 61  YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLS 120

Query: 121 YVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVG 180
           YVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVG
Sbjct: 121 YVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVG 180

Query: 181 IGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGI 240
           IGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGI
Sbjct: 181 IGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGI 240

Query: 241 IVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ 300
           IVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ
Sbjct: 241 IVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ 300

Query: 301 ESGKTNPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP 360
           ESGK NPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP
Sbjct: 301 ESGKINPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP 360

Query: 361 FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEI 420
           FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEI
Sbjct: 361 FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEI 420

Query: 421 SESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNY 480
           SESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATY NY
Sbjct: 421 SESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCNY 480

Query: 481 RDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNIK 540
           RDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNIK
Sbjct: 481 RDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNIK 540

Query: 541 YSAI 545
           YSAI
Sbjct: 541 YSAI 544

BLAST of CSPI01G24720 vs. NCBI nr
Match: gi|659068681|ref|XP_008445658.1| (PREDICTED: cannabidiolic acid synthase-like 1 [Cucumis melo])

HSP 1 Score: 1038.5 bits (2684), Expect = 4.2e-300
Identity = 509/544 (93.57%), Postives = 525/544 (96.51%), Query Frame = 1

Query: 1   MKSFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSF 60
           MKSF LTPFALFFIVL+F+SPSWAVSTK HEAFLQCL N SLTTNYSISNLIYTPINSSF
Sbjct: 3   MKSFLLTPFALFFIVLTFLSPSWAVSTKKHEAFLQCLFNKSLTTNYSISNLIYTPINSSF 62

Query: 61  YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLS 120
           YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQA I+CSKSHALQIRIRSGGHDFEGLS
Sbjct: 63  YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQAAIICSKSHALQIRIRSGGHDFEGLS 122

Query: 121 YVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVG 180
           YVSDVPFI+VDLINLRSITIDVE ENAWVQSGATLGEFYYRIGEKS+TLAFPAGSCPTVG
Sbjct: 123 YVSDVPFIVVDLINLRSITIDVEKENAWVQSGATLGEFYYRIGEKSRTLAFPAGSCPTVG 182

Query: 181 IGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGI 240
           IGGHLSGGGFGWLMRKYGLAADNVIDASF+DANGKVYDRESMG+DLFWAIRGGGGGSFGI
Sbjct: 183 IGGHLSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGGSFGI 242

Query: 241 IVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ 300
           IVAWKVKLVRVPATVTICGS+RSLEEEDTIKLIH+WQYITN LDKNLLLGISLTGGN TQ
Sbjct: 243 IVAWKVKLVRVPATVTICGSKRSLEEEDTIKLIHQWQYITNTLDKNLLLGISLTGGNLTQ 302

Query: 301 ESGKTNPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP 360
           ESGKTNP ALFSSFFLGKVNELMPIL+T FPELNLSKEECSEMSWIKTVL MAGFP  EP
Sbjct: 303 ESGKTNPNALFSSFFLGKVNELMPILDTKFPELNLSKEECSEMSWIKTVLVMAGFPTHEP 362

Query: 361 FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEI 420
           FEVLLNRTP FGLSTKIKSDYIKKPMSEAAFKTMLKRLKA+DIEVAQIMFIPYGGRMSEI
Sbjct: 363 FEVLLNRTPLFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAEDIEVAQIMFIPYGGRMSEI 422

Query: 421 SESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNY 480
           S+SE PFPHR GNIYKLGYYVKWKDQSIDAEKRHL+WIRDIYDYMTPFVSKSPRATYSNY
Sbjct: 423 SKSETPFPHRVGNIYKLGYYVKWKDQSIDAEKRHLSWIRDIYDYMTPFVSKSPRATYSNY 482

Query: 481 RDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNIK 540
           RDLDIG+NNKYGKATYSHARVWGFKYFGKNFDRLVH+KT IDPNDFFRNEQSIPAL +IK
Sbjct: 483 RDLDIGINNKYGKATYSHARVWGFKYFGKNFDRLVHVKTMIDPNDFFRNEQSIPALIDIK 542

Query: 541 YSAI 545
           YS I
Sbjct: 543 YSVI 546

BLAST of CSPI01G24720 vs. NCBI nr
Match: gi|778665253|ref|XP_004135742.2| (PREDICTED: cannabidiolic acid synthase-like 2 [Cucumis sativus])

HSP 1 Score: 753.8 bits (1945), Expect = 2.0e-214
Identity = 359/530 (67.74%), Postives = 433/530 (81.70%), Query Frame = 1

Query: 11  LFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNFSIQN 70
           +  +VLS  +PS+  ST+  E+F+QCL +   ++   I+NLIYTP NSS+ S+LNFSIQN
Sbjct: 19  IIVVVLSTATPSFC-STQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQN 78

Query: 71  LRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVPFIIV 130
           LRF  K  PKP AIITP HVSHIQA ++CSKSH  QIR RSGGHDFEGLSY+SD+PF++V
Sbjct: 79  LRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLV 138

Query: 131 DLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLSGGGF 190
           DLINL+SI+IDVEN+NAWV SGATLGE YYRI EKSQTLAFPAGSCPTVG+GGHLSGGG+
Sbjct: 139 DLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGY 198

Query: 191 GWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKVKLVR 250
           GWL+RKYGLAAD+VIDA  VDANGKV+++ESMG+DLFWAIRGGGGGSFGI+VAWKVKLV 
Sbjct: 199 GWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVE 258

Query: 251 VPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ-ESGKTNPTA 310
           VPA VTIC + R+LEE+  +KLIH+WQY+  KLD NLLLGISLTGG  T+ E GK NP A
Sbjct: 259 VPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIA 318

Query: 311 LFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPFEVLLNRTP 370
            FS  FLGK  E++ IL   FP+L L+KE+C EMSWI++VL M  F  ++P EVLLNR+ 
Sbjct: 319 SFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKEDPLEVLLNRSR 378

Query: 371 PFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEIPFPH 430
            +   +KIKSDY+K+ +   A K M +RLK+QD+E++QIMF+PYGG+MSE+ +SE PF H
Sbjct: 379 LYSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSH 438

Query: 431 RAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNYRDLDIGMNN 490
           R+   Y +GY  KWK+ S++AEK HLNWIR+IYDYMTPFVSKSPRA Y NYRDLDIG NN
Sbjct: 439 RSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNN 498

Query: 491 KYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNI 540
           KYG+ +Y  ARVWG KYFGKNFDRLVH+KTK+DP+DFFR+EQSIP L  I
Sbjct: 499 KYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTLSGI 547

BLAST of CSPI01G24720 vs. NCBI nr
Match: gi|659068701|ref|XP_008445757.1| (PREDICTED: cannabidiolic acid synthase-like 2 [Cucumis melo])

HSP 1 Score: 737.6 bits (1903), Expect = 1.5e-209
Identity = 357/544 (65.62%), Postives = 435/544 (79.96%), Query Frame = 1

Query: 1   MKSF--FLTPFAL--FFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPI 60
           MK F     PF L  F IV+   + + +  T+  ++F+QCL +     +  I+N+IYTP 
Sbjct: 1   MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS 60

Query: 61  NSSFYSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDF 120
           NSS+ S+LNFSIQNLRF  K   KPLAIITPSHVSHIQA ++CSKSH  QIR RSGGHDF
Sbjct: 61  NSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120

Query: 121 EGLSYVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSC 180
           EGLSY+S VPF++VDLINL SI+IDVEN+NAWV+SGATLGE YYRI EKSQTLAFPAGSC
Sbjct: 121 EGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSC 180

Query: 181 PTVGIGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGG 240
           PTVG+GGHLSGGG+GWL+RKYGLAAD+VIDA  VDANGKV+++ESMG+DLFWAIRGGGGG
Sbjct: 181 PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG 240

Query: 241 SFGIIVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGG 300
            FGI+VAWKVKLV VPA VTIC + R+LEE+  +KLIH+WQY+  KLD NLLLGISLTGG
Sbjct: 241 CFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG 300

Query: 301 NSTQ-ESGKTNPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGF 360
             T+ E GK NP A FS  FLGK  E++ IL   FP+L+L+KE+C EMSWI++VL M  F
Sbjct: 301 KVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWF 360

Query: 361 PNQEPFEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGG 420
              +P EVLLNRT  +   +KIKSDY+K+ +   A K M +RLK++D+E++QIMF+PYGG
Sbjct: 361 QKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGG 420

Query: 421 RMSEISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRA 480
           +MSE+ +SE PF HR+   Y +GY  KWK+ S++AE+RHLNWIR+IYDYMTPFVSKSPRA
Sbjct: 421 KMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRA 480

Query: 481 TYSNYRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPA 540
            Y NYRDLDIG NNKYG+ +Y  ARVWG KYFGKNFD+LV++KTK+DP+DFFR++QSIP 
Sbjct: 481 AYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPT 540

BLAST of CSPI01G24720 vs. NCBI nr
Match: gi|659068687|ref|XP_008445684.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 722.6 bits (1864), Expect = 5.1e-205
Identity = 352/532 (66.17%), Postives = 431/532 (81.02%), Query Frame = 1

Query: 6   LTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLN 65
           L P +L FIV+S  S   + S + ++ FLQCL  +S + +Y IS ++YTPINSS+ SVL+
Sbjct: 7   LIPLSLVFIVVSSASLCASASPQKYQNFLQCLSEHS-SKSYPISKVVYTPINSSYSSVLD 66

Query: 66  FSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDV 125
           FSI+NL FS+ +TPKPL IITPSHVSHIQA ++CSKSH LQIR RSGGHDFEGLSYV+  
Sbjct: 67  FSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYR 126

Query: 126 PFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHL 185
           PFI+VDLINLRS+ ++VEN  AWV+SGATLGE YYRIGEKS+TLAFPAG CPTVGIGGH 
Sbjct: 127 PFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGGHF 186

Query: 186 SGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWK 245
           SGGG+G ++RK+GLAADNVIDA  VDANGKV D+ESMG+DLFWAIRGGGGGSFGI+VAWK
Sbjct: 187 SGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFGIVVAWK 246

Query: 246 VKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKT 305
           +KLV VP TVTIC + R+L EED IKLIH+WQY+ NKLD+N+ LGI LTGGN++ ++G T
Sbjct: 247 IKLVPVPETVTICSTDRNL-EEDAIKLIHRWQYVGNKLDENIYLGIILTGGNASTQAGIT 306

Query: 306 NPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIK-TVLTMAGFPNQEPFEVL 365
           NPTA F S FLG+ +EL+  L+T FPEL L K++C E SW++ T++   G    E  E L
Sbjct: 307 NPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIETLEPL 366

Query: 366 LNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESE 425
           LNRT     STKIKSDYI +P+  AA K + +RLK QDIE +Q++F+PYGGRMS+ISESE
Sbjct: 367 LNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQISESE 426

Query: 426 IPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNYRDLD 485
            PF HRA  ++K+GY V WKDQS+ A+KRH++W+R++Y+YM PFVSKSPRA Y+NYRDLD
Sbjct: 427 TPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTNYRDLD 486

Query: 486 IGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
           IG NNKYGK +Y  A VWG KYFG NFDRLV++KTK+DP DFFR+EQSIP L
Sbjct: 487 IGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536