CsaV3_1G012540 (gene) Cucumber (Chinese Long) v3

NameCsaV3_1G012540
Typegene
OrganismCucumis sativus (Cucumber (Chinese Long) v3)
DescriptionMACPF domain-containing protein
Locationchr1 : 7799894 .. 7803817 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTTCAACATCAACCGGTTCAATCCTCAATCAGCCGCCGAGAAAGCGGTTTCAGTTATAGGCTTCGGCTACGATCTCTGTAACGATTTGCGATTGTCTTCTTGCAAGCCGGGTCCATCCGGGTCTAAACTGATCGATATTGATTTCACTCGAAGTAGAGACCTTGTACTTCCGGCCGGCGTTGTCGTCCCTAATGTTCCTACTTCCATCAACTGTGATAAAGGTGAGCGTACCCGATTCCGGTCGGATGTCATCTCCTTCAATCAGGTAATTTTCTTTGAAATTAGTTGTTGCTGATTTTATAGCTTAGAATTCTCTTCGTTTTATTGTTTTTTTTTTTTTTTTTTTTTCCATTTGTTGGCTTCATAAAACGAACTGGGTTTGTGTTTTTTCCGGTTAAAAACAGATGTCGGAGCTGTGTAATCAGCAATTATCTCTATCTGGGAAAATTCCGTCGGGGTTCTTTAACACCATGTTTGGATTAAAGGGTTGCTGGCAAAACGATGCAGGTTCCACAAGAAGTCTTGCATTTGATGGTTGTTTCATTACACTTTATAACATTGAGTTGGAAAAATCTCATATAACTCTCTCTGAGCAAGTCAAAAGGGAGGTTCCTTCTTCATGGGATCCTGCTGCCCTTGCAGAGTATGTTTCTTTTGTTTTTTGTTTTTATTTTTGTTTCCATTTTGTCTTTTCTTTTGTAGAAATCTAGACATTTGGATGTTTGTCTGTCCTCTTCTATTGAAATATCTTCATCACTGATATTTAACAAACTTCTATTTGTTCATCTTCTCCACATCAACACACTGATTGTTAATGCTGTTGAATGGATGAGAATGGATTTTTCGAAGTAATGTTTTCAACCATGCGCTTCTCTGTCCAACACTTGAGGAAGGACTGGTCTTTGAAATTGACTTGACTATGTTGCCAAAAAGGTGGAAAATGTGCATTTAGTTAGCTACTTTTATGTTTTACTATTCCAAAAGTAAATAAAAACAGTGATGGATGAGTACCTTTCTTGAAAGTTGATGGCTTGGTATGAACCTATTCTTTTAAGTCCTTCGCTTTTTCCCCTTTGTCCTAACAATCACTAGGTCATTTCATGGTCGTCGGTTCTACTGAACTTCCTAACTGTCAGCTTAAAGGATGGTCTAATTAGTATAATCCCTTAGATTCATCTAATATATCATACAAGACACGTTTTGTTCACGAGTTCTTTGGTTTAGATTACCTAAGTGTTTGTTGGGTACGTCTCATAATTACCTATATGTGTTTTTGTTGCATTTGAAACATTGTTTATCGAAGACATTACACAAACACTATCACGTTAGACATTAGGGACATATCAAATACTTGTTAAAGTGAAACACATTGGATATCTGATTTTTGTGCCAACTAAATACTAACTTGATTTCAGGTTCATTGAGAAATATGGTACTCATGTGGTTGTGGGTGTAAAAATGGGAGGAAAAGATGTCATTTTCATGAGGCAATTACGAGAATCGAATGTAGAGCAAACCGATGTGCAGAAGAAGTTAAAGAAATTAGCAGATGATAAATTTTCTGAAGATGCAAATGGAGGTTTAACGTCCAATCCTGTTGCAGCAAAAATCAAGGTACACACTCGAGAACATAAATGGCTATCACCATTTGGTCTATTCCATTGGTCCCATGTCAAAATAGAGGATGTTGATTAGCTATTGACGGAAATTAAAGAATCAATTTTCCTTGAGTCATGCATTGTATGCAGCTCTAGATTCATGTTCTTTCCTTAAAATTCTTAGATTGAGATAAAATCATGTCAATAGAATATGGTTTAGTTGCTAAGTTCCGTATAGAATGTGGTTTAATTGTTACCTGATATAGCAGTTATCATGCCTTTATCATCAGCTTGGCTTGCTTGTGAATTCTTCTTCTACTTCTGCATCTACACATATACTAAATATAACTCAACTAAGATTTATAAAAACTCCATAAACATGGCTAGTCAGATAATAATCTTTGCTAATTGTATTTAAATTAATCACTATAACCATGGGGCATACAATTTCTGTTCCAATACATTATTCTGTTTTTAAAACAATTTTGATAGGATAAATGATTCGCAATGTCATATTATTCACATAGAACAGAAAATTTAAAAGTGCAAACAAGTTTCGTGGTTTAATGATGCATCTACTAACGACATTTTCTATATGTATAAACATTTTAAGTCTCATCAGGAGAAATAAACTTTTATCCTTAATGATTTCAATTTTTAGGATGAATATTCTCTGCCATGGGAGCTTCGTAATGCATTTGCTGCCTCTATCAAACCACCAGTGATCAATCACTCAAAGGGTGATGTAAGCATCTCGGCATCTACCTGTTCGTTCGAGGAGAATATTGCTACACTTTCGACCTATTAAGCAGATGAGAACTGACTTATTTAATTAAACAACTGGATGCAGGAATTAACAGCAATTTATATTAGAAGAGGAGGTGTTGATGTTGGTCAAAGTCATAATCAATGGCTCTCAACCATTTCTCAGTCACCTAATGTTATCTCAATGTCCTTTGTACCAATAACATCACTGATGAATGGCGTCCGAGGCAATGGATTTCTAAGCCATGCCGTAAACCTGTATCTAAGATGTAAGTTGCAAGTCTTGCTTTTAAGTGTTGTTTCTCTACAGCGAATGGACCTGGATAATTTTTGTCCTTTAACGAGAACCTGAAATATGGAGATCAGATACGTACATTGTGCGTCCTTAAAATTTGACCTTAGTTACTCTTCCCACGGTGGCTGTAGTACTTGAAGCTTCCATTAAAAGATTTTGACCCAAGGTTCATGCCAACAAGGTACCGTTCTTATTAAGGTTAGTGTTAGATTTTGATCATTGAAAAATTGTGCAGATAAACCTCCAATTGAGGAGCTCCAGCAATTTCTTGAATTTCAACTGCCTAGACAGTGGGCGCCGGTGTACGCTGATCTTCCTCTTGCTCCTCGGCATAGGAAACAAGCCTCTCCATCTCTCAAGTTTACTTTGATGGGGCCCAAGCTTTATGTAAATACTACTAAGGTAATCGTCTGACTTGAATATTTGGGTCAGTCAAAAACTTCAAATAGCAATGGCAATGGCAATGTCGCAAATGTGATCACTGCTTGATTTTTGCCTGACCTATCTATTACCTTTGCTTGGATGCAAATTGTTGAAAATTTCACATGGTGTCACAATTTTGAAACTGCAGGTTGATTCAAACAATCTTCCAGTAACGGGAGTTCGCTTGTTTTTGGAAGGAAAGAAAAGTGACCATCTGGCAATTCATCTCCAGCATCTATCAACTCTTCCCAAGACTCTTCAACTCTCAGATGATCTAAGCTATCGACCCATCGACGAGCCAGAGGAGAAGGGCTACTTCGAACCAGTTAAATGGAGCATATTCTCTCATGTCTGCACTGCTCCAGTAGAGTACAATGGTTCTCTAATTGATGATGACTCTGCTTCTATCGTCACAAAGGCATGGTTTGAAGTTAAGGGTATTGGAATGAGAAAGGTCTTGTTCCTGAGGCTCGGGTTTTCGATGGTGGCATCAGCAGGGATTCGTCGATCGGACTGGGAAGGACCTTCAACAACATCCCGAAAATCTGGACTCATCTCCACCTTTATCAGCACAAGGTTTAGTGCAGGGTTGCATCAACCAGAAGAGCAACCAAAAATTGAATTGAATTCGGCTGTTTATCCTGATGGTCCACCATTGACTTCTAGGGCAATGAAAATGTTGAATGTCATCGATACGAAAGAAATGGTGCGTGGTCCTGAGAATTCACCTGGATATTGGGTGGTTACCGGAGCCAAGCTGTGTGTGGAAGGTGGCCGGATCTCTATCAAAGTGAAATACTCTTTGTTAACCATATTGTCGGAGGATTCTATGATCTGA

mRNA sequence

ATGTCTTTCAACATCAACCGGTTCAATCCTCAATCAGCCGCCGAGAAAGCGGTTTCAGTTATAGGCTTCGGCTACGATCTCTGTAACGATTTGCGATTGTCTTCTTGCAAGCCGGGTCCATCCGGGTCTAAACTGATCGATATTGATTTCACTCGAAGTAGAGACCTTGTACTTCCGGCCGGCGTTGTCGTCCCTAATGTTCCTACTTCCATCAACTGTGATAAAGGTGAGCGTACCCGATTCCGGTCGGATGTCATCTCCTTCAATCAGATGTCGGAGCTGTGTAATCAGCAATTATCTCTATCTGGGAAAATTCCGTCGGGGTTCTTTAACACCATGTTTGGATTAAAGGGTTGCTGGCAAAACGATGCAGGTTCCACAAGAAGTCTTGCATTTGATGGTTGTTTCATTACACTTTATAACATTGAGTTGGAAAAATCTCATATAACTCTCTCTGAGCAAGTCAAAAGGGAGGTTCCTTCTTCATGGGATCCTGCTGCCCTTGCAGAGTTCATTGAGAAATATGGTACTCATGTGGTTGTGGGTGTAAAAATGGGAGGAAAAGATGTCATTTTCATGAGGCAATTACGAGAATCGAATGTAGAGCAAACCGATGTGCAGAAGAAGTTAAAGAAATTAGCAGATGATAAATTTTCTGAAGATGCAAATGGAGGTTTAACGTCCAATCCTGTTGCAGCAAAAATCAAGGATGAATATTCTCTGCCATGGGAGCTTCGTAATGCATTTGCTGCCTCTATCAAACCACCAGTGATCAATCACTCAAAGGGTGATGAATTAACAGCAATTTATATTAGAAGAGGAGGTGTTGATGTTGGTCAAAGTCATAATCAATGGCTCTCAACCATTTCTCAGTCACCTAATGTTATCTCAATGTCCTTTGTACCAATAACATCACTGATGAATGGCGTCCGAGGCAATGGATTTCTAAGCCATGCCGTAAACCTGTATCTAAGATATAAACCTCCAATTGAGGAGCTCCAGCAATTTCTTGAATTTCAACTGCCTAGACAGTGGGCGCCGGTGTACGCTGATCTTCCTCTTGCTCCTCGGCATAGGAAACAAGCCTCTCCATCTCTCAAGTTTACTTTGATGGGGCCCAAGCTTTATGTAAATACTACTAAGGTTGATTCAAACAATCTTCCAGTAACGGGAGTTCGCTTGTTTTTGGAAGGAAAGAAAAGTGACCATCTGGCAATTCATCTCCAGCATCTATCAACTCTTCCCAAGACTCTTCAACTCTCAGATGATCTAAGCTATCGACCCATCGACGAGCCAGAGGAGAAGGGCTACTTCGAACCAGTTAAATGGAGCATATTCTCTCATGTCTGCACTGCTCCAGTAGAGTACAATGGTTCTCTAATTGATGATGACTCTGCTTCTATCGTCACAAAGGCATGGTTTGAAGTTAAGGGTATTGGAATGAGAAAGGTCTTGTTCCTGAGGCTCGGGTTTTCGATGGTGGCATCAGCAGGGATTCGTCGATCGGACTGGGAAGGACCTTCAACAACATCCCGAAAATCTGGACTCATCTCCACCTTTATCAGCACAAGGTTTAGTGCAGGGTTGCATCAACCAGAAGAGCAACCAAAAATTGAATTGAATTCGGCTGTTTATCCTGATGGTCCACCATTGACTTCTAGGGCAATGAAAATGTTGAATGTCATCGATACGAAAGAAATGGTGCGTGGTCCTGAGAATTCACCTGGATATTGGGTGGTTACCGGAGCCAAGCTGTGTGTGGAAGGTGGCCGGATCTCTATCAAAGTGAAATACTCTTTGTTAACCATATTGTCGGAGGATTCTATGATCTGA

Coding sequence (CDS)

ATGTCTTTCAACATCAACCGGTTCAATCCTCAATCAGCCGCCGAGAAAGCGGTTTCAGTTATAGGCTTCGGCTACGATCTCTGTAACGATTTGCGATTGTCTTCTTGCAAGCCGGGTCCATCCGGGTCTAAACTGATCGATATTGATTTCACTCGAAGTAGAGACCTTGTACTTCCGGCCGGCGTTGTCGTCCCTAATGTTCCTACTTCCATCAACTGTGATAAAGGTGAGCGTACCCGATTCCGGTCGGATGTCATCTCCTTCAATCAGATGTCGGAGCTGTGTAATCAGCAATTATCTCTATCTGGGAAAATTCCGTCGGGGTTCTTTAACACCATGTTTGGATTAAAGGGTTGCTGGCAAAACGATGCAGGTTCCACAAGAAGTCTTGCATTTGATGGTTGTTTCATTACACTTTATAACATTGAGTTGGAAAAATCTCATATAACTCTCTCTGAGCAAGTCAAAAGGGAGGTTCCTTCTTCATGGGATCCTGCTGCCCTTGCAGAGTTCATTGAGAAATATGGTACTCATGTGGTTGTGGGTGTAAAAATGGGAGGAAAAGATGTCATTTTCATGAGGCAATTACGAGAATCGAATGTAGAGCAAACCGATGTGCAGAAGAAGTTAAAGAAATTAGCAGATGATAAATTTTCTGAAGATGCAAATGGAGGTTTAACGTCCAATCCTGTTGCAGCAAAAATCAAGGATGAATATTCTCTGCCATGGGAGCTTCGTAATGCATTTGCTGCCTCTATCAAACCACCAGTGATCAATCACTCAAAGGGTGATGAATTAACAGCAATTTATATTAGAAGAGGAGGTGTTGATGTTGGTCAAAGTCATAATCAATGGCTCTCAACCATTTCTCAGTCACCTAATGTTATCTCAATGTCCTTTGTACCAATAACATCACTGATGAATGGCGTCCGAGGCAATGGATTTCTAAGCCATGCCGTAAACCTGTATCTAAGATATAAACCTCCAATTGAGGAGCTCCAGCAATTTCTTGAATTTCAACTGCCTAGACAGTGGGCGCCGGTGTACGCTGATCTTCCTCTTGCTCCTCGGCATAGGAAACAAGCCTCTCCATCTCTCAAGTTTACTTTGATGGGGCCCAAGCTTTATGTAAATACTACTAAGGTTGATTCAAACAATCTTCCAGTAACGGGAGTTCGCTTGTTTTTGGAAGGAAAGAAAAGTGACCATCTGGCAATTCATCTCCAGCATCTATCAACTCTTCCCAAGACTCTTCAACTCTCAGATGATCTAAGCTATCGACCCATCGACGAGCCAGAGGAGAAGGGCTACTTCGAACCAGTTAAATGGAGCATATTCTCTCATGTCTGCACTGCTCCAGTAGAGTACAATGGTTCTCTAATTGATGATGACTCTGCTTCTATCGTCACAAAGGCATGGTTTGAAGTTAAGGGTATTGGAATGAGAAAGGTCTTGTTCCTGAGGCTCGGGTTTTCGATGGTGGCATCAGCAGGGATTCGTCGATCGGACTGGGAAGGACCTTCAACAACATCCCGAAAATCTGGACTCATCTCCACCTTTATCAGCACAAGGTTTAGTGCAGGGTTGCATCAACCAGAAGAGCAACCAAAAATTGAATTGAATTCGGCTGTTTATCCTGATGGTCCACCATTGACTTCTAGGGCAATGAAAATGTTGAATGTCATCGATACGAAAGAAATGGTGCGTGGTCCTGAGAATTCACCTGGATATTGGGTGGTTACCGGAGCCAAGCTGTGTGTGGAAGGTGGCCGGATCTCTATCAAAGTGAAATACTCTTTGTTAACCATATTGTCGGAGGATTCTATGATCTGA

Protein sequence

MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYSLPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGMRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLLTILSEDSMI
BLAST of CsaV3_1G012540 vs. NCBI nr
Match: XP_004146657.1 (PREDICTED: MACPF domain-containing protein NSL1 [Cucumis sativus] >KGN64702.1 hypothetical protein Csa_1G075560 [Cucumis sativus])

HSP 1 Score: 1235.7 bits (3196), Expect = 0.0e+00
Identity = 610/610 (100.00%), Postives = 610/610 (100.00%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480
           SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of CsaV3_1G012540 vs. NCBI nr
Match: XP_022994356.1 (MACPF domain-containing protein NSL1 [Cucurbita maxima])

HSP 1 Score: 1137.1 bits (2940), Expect = 0.0e+00
Identity = 555/610 (90.98%), Postives = 583/610 (95.57%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFN+NRF+PQSAAEKAVSVIGFG+DLCNDLRLSSCKPGPSGSKLIDID TR++DLVLPA
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNV  SI CDKGERTRFRSDVISFNQMSEL NQQLSLSGKIPS FFN+MFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGS++SL FDGCFITLYNIELE+SHITLSEQVKREVPSSWDPAALA+FIE YGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALAKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESN+EQT+VQKKLK+LAD++FSEDANGG  SN  AAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQTEVQKKLKQLADERFSEDANGGFMSNSAAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           +PW+LR+AFAASIKPP+I HSKGDEL  IY+RRGGVDVGQSHNQWLST+SQSPNVI MSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELMTIYVRRGGVDVGQSHNQWLSTVSQSPNVILMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLA RHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSN+ PVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480
           SDDLSY PIDEPEEKGYF+PVKWSIFSHVCTAPV+YNGSLIDDD+ASIVTKAWFEVK IG
Sbjct: 421 SDDLSYGPIDEPEEKGYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFS VASA IRRS+WEGPST+SRKSGLISTFISTRFS GL QPE QPKIEL
Sbjct: 481 MRKVLFLRLGFSTVASAMIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLT RAMKMLN+IDTKEMVRGPENSPGYWVVTGAKLCVEG RISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTVRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of CsaV3_1G012540 vs. NCBI nr
Match: XP_023542863.1 (MACPF domain-containing protein NSL1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1132.9 bits (2929), Expect = 0.0e+00
Identity = 552/610 (90.49%), Postives = 583/610 (95.57%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFN+NRF+PQSAAEKAVSVIGFG+DLCNDLRLSSCKPGPSGSKLIDID TR++DLVLPA
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNV  SI CDKGERTRFRSDVISFNQMSEL NQQLSLSGKIPS FFN+MFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGS++SL FDGCFITLYNIELE+SHITLSEQVKREVPSSWDPAALA+FIE YGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALAKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRES++EQ +VQKKLK+LAD++FSEDANGG  SN  AAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESHIEQAEVQKKLKQLADERFSEDANGGFMSNSAAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           +PW+LR+AFAASIKPP+I HSKGDEL  IY+RRGGVDVGQSHNQWLST+SQSPNVISMSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELMTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSL+NGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLA RHRK
Sbjct: 301 VPITSLLNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSN+ PVTGVRLFLEGKKSDHLAIHLQHLSTLPKT+QL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480
           SDDLSY PIDEPEEKGYF+PVKWSIFSHVCTAPV+YNGSLIDDD+ASIVTKAWFEVK IG
Sbjct: 421 SDDLSYGPIDEPEEKGYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFS VASA IRRS+WEGPST+SRKSGLISTFISTRFS GL QPE QPKIEL
Sbjct: 481 MRKVLFLRLGFSTVASAMIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLT RAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEG RISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTVRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTIL+EDSMI
Sbjct: 601 LTILTEDSMI 610

BLAST of CsaV3_1G012540 vs. NCBI nr
Match: XP_022955164.1 (MACPF domain-containing protein NSL1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1132.5 bits (2928), Expect = 0.0e+00
Identity = 552/610 (90.49%), Postives = 582/610 (95.41%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFN+NRF+PQSAAEKAVSVIGFG+DLCNDLRLSSCKPGPSGSKLIDID TR++DLVLPA
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNV  SI CDKGERTRFRSDV+SFNQMSEL NQQLSLSGKIPS FFN+MFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVVSFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGS++SL FDGCFITLYNIELE+SHITLSEQVKREVPSSWDPAALA+FIE YGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALAKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESN+EQ +VQKKLK+LAD++FSED NGG  SN  AAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDVNGGFMSNSTAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           +PW+LR+AFAASIKPP+I HSKGDELT IY+RRGGVDVGQSHNQWLST+SQSPNVISMSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSL+NGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLA R RK
Sbjct: 301 VPITSLLNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRLRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSN+ PVTGVRLFLEGKKSDHLAIHLQHLSTLPKT+QL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480
           SDDLSY PIDEPEEKGYF+PVKWSIFSHVCTAPV+YNGSLIDDD+ASIVTKAWFEVK IG
Sbjct: 421 SDDLSYGPIDEPEEKGYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFS VASA IRRS+WEGPST+SRKSGLISTFISTRFS GL QPE QPKIEL
Sbjct: 481 MRKVLFLRLGFSTVASAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLT RAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEG RISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTVRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of CsaV3_1G012540 vs. NCBI nr
Match: XP_022140066.1 (MACPF domain-containing protein NSL1 [Momordica charantia])

HSP 1 Score: 1121.3 bits (2899), Expect = 0.0e+00
Identity = 544/612 (88.89%), Postives = 582/612 (95.10%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFN++RF+PQSAAEKAVSV+GFGYDLCND+RLSSCKPGPSGSKLI +D TR++DLVLP 
Sbjct: 1   MSFNVHRFDPQSAAEKAVSVLGFGYDLCNDIRLSSCKPGPSGSKLIHLDPTRTKDLVLPP 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120
           G+VVPNV TSI CDKGERTRFRSDVISFNQMSE  NQQL LSGKIPSGFFN MF  KG W
Sbjct: 61  GIVVPNVSTSIKCDKGERTRFRSDVISFNQMSEQFNQQLCLSGKIPSGFFNAMFNFKGNW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGST+SLAFDGCFITLYNIELE+SHITLSEQ+KREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTKSLAFDGCFITLYNIELERSHITLSEQLKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDAN--GGLTSNPVAAKIKDE 240
           VGVKMGGKDVI+MRQLRESN+EQ +VQK LK+LADD+FSEDAN  G  TS+P A KIKDE
Sbjct: 181 VGVKMGGKDVIYMRQLRESNLEQNEVQKTLKQLADDRFSEDANATGSFTSHPAAPKIKDE 240

Query: 241 YSLPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISM 300
           YS+PWELRNAFAASIKPP+++HSK DEL  I++RRGGVD+GQSHNQWLSTISQSPNVISM
Sbjct: 241 YSVPWELRNAFAASIKPPIVSHSKSDELMKIFVRRGGVDIGQSHNQWLSTISQSPNVISM 300

Query: 301 SFVPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRH 360
           SFVPITSLMNG+RGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLA RH
Sbjct: 301 SFVPITSLMNGIRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRH 360

Query: 361 RKQASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTL 420
           R QASPSLKFTLMGP+LYVNTTKVD+ N PVTG+RLFLEGKKSDHLA+HLQHLS+LPKTL
Sbjct: 361 RNQASPSLKFTLMGPRLYVNTTKVDAQNRPVTGIRLFLEGKKSDHLAVHLQHLSSLPKTL 420

Query: 421 QLSDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKG 480
           QLSDDLSY+PIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVK 
Sbjct: 421 QLSDDLSYKPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKV 480

Query: 481 IGMRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKI 540
           +GMRKVLFLRLGFSMVASA IRRS+WEGPSTTSRKSGLISTFISTRFS GLHQPE+QPKI
Sbjct: 481 VGMRKVLFLRLGFSMVASAVIRRSEWEGPSTTSRKSGLISTFISTRFSTGLHQPEKQPKI 540

Query: 541 ELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKY 600
           ELNSAVYPDGPPLTSRAMKMLNVI+TKEMVRGPENSPGYWVVTGAKLCVEGGRIS+KVKY
Sbjct: 541 ELNSAVYPDGPPLTSRAMKMLNVINTKEMVRGPENSPGYWVVTGAKLCVEGGRISVKVKY 600

Query: 601 SLLTILSEDSMI 611
           +LLTILSEDSMI
Sbjct: 601 ALLTILSEDSMI 612

BLAST of CsaV3_1G012540 vs. TAIR10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein)

HSP 1 Score: 788.1 bits (2034), Expect = 3.9e-228
Identity = 390/612 (63.73%), Postives = 474/612 (77.45%), Query Frame = 0

Query: 4   NINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVV 63
           N  R +  SAAEKAVSVIG GYDLC+D+R S+CK  P GS+L++ID TR+RDL+ P G+V
Sbjct: 5   NFTRLDAHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIV 64

Query: 64  VPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQND 123
           V NV +SI CDKGERTR RSD++SFNQMSE  NQ + LSGKIPSG FN MF    CW  D
Sbjct: 65  VNNVSSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKD 124

Query: 124 AGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGV 183
           A S ++LA+DG FI+LY++E+ +  +TL ++VKREVPSSWD AALA FIEKYGTHVVVGV
Sbjct: 125 ASSVKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGV 184

Query: 184 KMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSED-ANGGLTSNPVAAKIKDEYSLP 243
            MGGKDVI ++Q+R+SN E  ++QK LK   D++F  D       ++  + K K+E  L 
Sbjct: 185 TMGGKDVIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQ 244

Query: 244 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVP 303
           W L+  F  S+   V+ H+K +E+  + IRRGGVD+GQSH +WLST+SQ+PNVISM FVP
Sbjct: 245 WGLQ-PFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVP 304

Query: 304 ITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQA 363
           ITSL++G+ G GFLSHAVNLYLRYKPPIEEL QFLEFQLPRQWAPVY DLPL  R  KQ+
Sbjct: 305 ITSLLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQS 364

Query: 364 SPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSD 423
           SPSL+F+LMGPKLYVNT+KVDS   PVTG+R FLEGKK +HLAIHLQHLS  P +L LS 
Sbjct: 365 SPSLQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSH 424

Query: 424 DLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGMR 483
           D +Y PI+EP EKGY+ PVKW IFSHVCT PV+YNG+   DD+ASIVTKAW EVKG+GMR
Sbjct: 425 DDTYEPIEEPVEKGYYVPVKWGIFSHVCTYPVQYNGAR-SDDTASIVTKAWLEVKGMGMR 484

Query: 484 KVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLH----QPEEQPKI 543
           KVLFLRLGFS+ ASA  R+S W+  ST SRKSG+ S  ISTR S GL       + Q KI
Sbjct: 485 KVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKI 544

Query: 544 ELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKY 603
           ++NSAVYP GP    +  K+L+++DTKE++RGPE  PGYWVVTGAKLCVE G+ISIK KY
Sbjct: 545 DINSAVYPRGPSPPVKP-KLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKY 604

Query: 604 SLLTILSEDSMI 611
           SLLT++SEDS++
Sbjct: 605 SLLTVISEDSLV 612

BLAST of CsaV3_1G012540 vs. TAIR10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein)

HSP 1 Score: 626.3 bits (1614), Expect = 2.0e-179
Identity = 318/610 (52.13%), Postives = 419/610 (68.69%), Query Frame = 0

Query: 7   RFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDI-DFTRSRDLVLPAGVVVP 66
           R     AAE A+  IG GYDL  DLRL  CK G   S+L+DI +   + ++VLP G+ +P
Sbjct: 4   RLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIP 63

Query: 67  NVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAG 126
           NV  SI CDKGER RFRSD++ F QM+E  NQ+LSL+GKIPSG FN MF    CWQ DA 
Sbjct: 64  NVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAA 123

Query: 127 STRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKM 186
            T++LAFDG FI+LY++ L+KS + L E VK+ VPS+WDPAALA FI+ YGTH++V VKM
Sbjct: 124 YTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKM 183

Query: 187 GGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDA---NGGLTSNPVAAKIKDEYSLP 246
           GGKDVI+ +Q   S ++  D+QK+LK++AD +F E +   N G      ++K++ +    
Sbjct: 184 GGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQ-- 243

Query: 247 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVD-VGQSHNQWLSTISQSPNVISMSFV 306
             LR A  +S+     +++  ++   +  RRGG D     HN+WL T+   P+VISMSF+
Sbjct: 244 -RLRFADTSSLG----SYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFI 303

Query: 307 PITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQ 366
           PITSL+NGV G+GFLSHA+NLYLRYKPPIEEL QFLEFQLPRQWAPV+++LPL P+ ++Q
Sbjct: 304 PITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQ 363

Query: 367 ASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLS 426
           +  SL+F+  GPKLYVNTT VD    P+TG+RL+LEG++S+ LAIHLQHLS+LPK  QL 
Sbjct: 364 SCASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLE 423

Query: 427 DDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGM 486
           DDL+     E  ++ Y+E V W  +SHVCT PVE       DD  S+VT A   V+  G 
Sbjct: 424 DDLNRSIRQESHDRRYYEKVNWKNYSHVCTEPVE------SDDDLSVVTGAQLHVESHGF 483

Query: 487 RKVLFLRLGFSMVASAG-IRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 546
           + VLFLRL FS V  A  ++ S+W+     + KSGLIST IS  F+A    P     + +
Sbjct: 484 KNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNI 543

Query: 547 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 606
           NSA+YP GPP+ ++A K+L  +DT EM RGP+ SPGYWVV+GA+L VE G+IS+KVKYSL
Sbjct: 544 NSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 600

Query: 607 LTILSEDSMI 611
            T +  D +I
Sbjct: 604 FTPILGDEVI 600

BLAST of CsaV3_1G012540 vs. TAIR10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein)

HSP 1 Score: 463.8 bits (1192), Expect = 1.7e-130
Identity = 257/614 (41.86%), Postives = 376/614 (61.24%), Query Frame = 0

Query: 15  EKAVSVIGFGYDLCNDLRLSSCKPGPSGS---KLIDIDFTRSRDLVLPAGVVVPNVPTSI 74
           E AV  +G G+DL  D RL  CK G   +   +L+ +D T++R+L +P   V  NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 75  NCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLK-GCWQNDAGSTRSL 134
           NCDKGERTRFRSD++ FN+MSE  NQ+ S++GKIPSG FN  FG + G W  DA + +SL
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 135 AFDGCFITLYNIELEK-SHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKD 194
             D   +TL+N+ +   + + L+++V+  VPSSWDP  LA FIE+YGTHV+ GV +GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 195 VIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYSLPW--ELRN 254
           V+ +RQ + S+++   ++  L  L D  F+      L++  +       +S P   E  N
Sbjct: 190 VVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCL--LSTRRLNKAYHHSHSQPKFPEAFN 249

Query: 255 AF----AASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPI 314
            F      +     IN   G  +T I  +RGG    +SH++WL T+   P+ I+ +F+PI
Sbjct: 250 VFDDKQTVAFNNFSINSQNG--ITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPI 309

Query: 315 TSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPL-APRHRKQA 374
           TSL+  V G+G LSHA++LYLRYKPP+ +LQ FL+F  PR WAPV+ DLP  A  +   A
Sbjct: 310 TSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASA 369

Query: 375 SPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTL--QL 434
            P+L    MGPKLYVNTT V S   PVTG+R FLEGKK + LAIHLQHL     T+  ++
Sbjct: 370 YPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKI 429

Query: 435 SDDLSYRPIDE-PEEKGYFEPVKWSIFSHVCTAPVEYNGSLI--------DDDSASIVTK 494
           +D+  +R  D+  +   YFEP+    FSHVCT PV+Y+ + I         +D A IVT 
Sbjct: 430 TDEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTG 489

Query: 495 AWFEVKGIGMRKVLFLRLGFSMVASAGIRRSDW-EGPSTTSRKSGLISTFISTRFSAGLH 554
           A  EVK  G + VL LRL ++ V+   + ++ W  GP  TS+KSG+ S+      S  +H
Sbjct: 490 AQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVH 549

Query: 555 Q---PEEQPKIELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCV 602
                +++ ++ L+S V+P GPP+ +   K++  +D  ++ RGP++SPG+W+VTG +L +
Sbjct: 550 HNMIQKDKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTGVRLYL 609

BLAST of CsaV3_1G012540 vs. TAIR10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein)

HSP 1 Score: 425.2 bits (1092), Expect = 6.6e-119
Identity = 238/600 (39.67%), Postives = 348/600 (58.00%), Query Frame = 0

Query: 17  AVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVVVPNVPTSINCDKG 76
           A+  +G G+D+ +D+RL  CK  P GS+L+ I+  ++RDL L  G ++PNVP  I+C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKGAP-GSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 77  ERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAGSTRSLAFDGCF 136
                R  V SF++M+E  N +  + G IP G FN MF   G WQ DA ST+SLA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 137 ITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKDVIFMRQL 196
           I LY+++L K  + L  +++R VPSSWDPA+LA FIE YGTH+V  V +GG+DV+++RQ 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 197 RESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYSLPWELRNAFAASIKPP 256
           + S +  ++++  +  +   +F E  +  +T  P+  K KD                   
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHEAESQSIT-GPLKYKDKD------------------- 263

Query: 257 VINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITSLMNGVRGNGFL 316
                    +T I+ RRGG D+ QSH +W  T+  +P++I+M+F PI SL+ GV G   L
Sbjct: 264 ---------ITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHL 323

Query: 317 SHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQASPSLKFTLMGPKLY 376
           + A+ LYL YKPPIE+LQ FL++Q+ R WAP  ++L    + ++    SL+F+LMGPKL+
Sbjct: 324 TRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLF 383

Query: 377 VNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDDLSYRPIDEPEEKG 436
           ++  +V     PVTG+RL LEG K + L+IHLQHL +LPK LQ   D S+ PI  P+ +G
Sbjct: 384 ISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWD-SHVPIGAPKWQG 443

Query: 437 -------YFEPVKWSIFSHVCTAPVEYNGSLIDD-DSASIVTKAWFEVKGIGMRKVLFLR 496
                  +FEP+KW  FSHV T+P+E+  + I D     IVT A   V   G + VL L+
Sbjct: 444 PEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLK 503

Query: 497 LGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELNSAVYPDG 556
           L FS V    IRRS W+                        H P     +  +  + P G
Sbjct: 504 LLFSKVPGCTIRRSVWD------------------------HTP-----VASSGRLEPGG 559

Query: 557 PPLTS-------RAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLL 602
           P  +S       ++ K+  ++D+ EM++GP++ PG+W+VTGAKL VE G+I ++VKYSLL
Sbjct: 564 PSTSSSTEEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559

BLAST of CsaV3_1G012540 vs. Swiss-Prot
Match: sp|Q9SGN6|NSL1_ARATH (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 7.0e-227
Identity = 390/612 (63.73%), Postives = 474/612 (77.45%), Query Frame = 0

Query: 4   NINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVV 63
           N  R +  SAAEKAVSVIG GYDLC+D+R S+CK  P GS+L++ID TR+RDL+ P G+V
Sbjct: 5   NFTRLDAHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIV 64

Query: 64  VPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQND 123
           V NV +SI CDKGERTR RSD++SFNQMSE  NQ + LSGKIPSG FN MF    CW  D
Sbjct: 65  VNNVSSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKD 124

Query: 124 AGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGV 183
           A S ++LA+DG FI+LY++E+ +  +TL ++VKREVPSSWD AALA FIEKYGTHVVVGV
Sbjct: 125 ASSVKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGV 184

Query: 184 KMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSED-ANGGLTSNPVAAKIKDEYSLP 243
            MGGKDVI ++Q+R+SN E  ++QK LK   D++F  D       ++  + K K+E  L 
Sbjct: 185 TMGGKDVIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQ 244

Query: 244 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVP 303
           W L+  F  S+   V+ H+K +E+  + IRRGGVD+GQSH +WLST+SQ+PNVISM FVP
Sbjct: 245 WGLQ-PFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVP 304

Query: 304 ITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQA 363
           ITSL++G+ G GFLSHAVNLYLRYKPPIEEL QFLEFQLPRQWAPVY DLPL  R  KQ+
Sbjct: 305 ITSLLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQS 364

Query: 364 SPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSD 423
           SPSL+F+LMGPKLYVNT+KVDS   PVTG+R FLEGKK +HLAIHLQHLS  P +L LS 
Sbjct: 365 SPSLQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSH 424

Query: 424 DLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGMR 483
           D +Y PI+EP EKGY+ PVKW IFSHVCT PV+YNG+   DD+ASIVTKAW EVKG+GMR
Sbjct: 425 DDTYEPIEEPVEKGYYVPVKWGIFSHVCTYPVQYNGAR-SDDTASIVTKAWLEVKGMGMR 484

Query: 484 KVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLH----QPEEQPKI 543
           KVLFLRLGFS+ ASA  R+S W+  ST SRKSG+ S  ISTR S GL       + Q KI
Sbjct: 485 KVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKI 544

Query: 544 ELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKY 603
           ++NSAVYP GP    +  K+L+++DTKE++RGPE  PGYWVVTGAKLCVE G+ISIK KY
Sbjct: 545 DINSAVYPRGPSPPVKP-KLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKY 604

Query: 604 SLLTILSEDSMI 611
           SLLT++SEDS++
Sbjct: 605 SLLTVISEDSLV 612

BLAST of CsaV3_1G012540 vs. Swiss-Prot
Match: sp|Q9STW5|MACP2_ARATH (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 3.5e-178
Identity = 318/610 (52.13%), Postives = 419/610 (68.69%), Query Frame = 0

Query: 7   RFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDI-DFTRSRDLVLPAGVVVP 66
           R     AAE A+  IG GYDL  DLRL  CK G   S+L+DI +   + ++VLP G+ +P
Sbjct: 4   RLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIP 63

Query: 67  NVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAG 126
           NV  SI CDKGER RFRSD++ F QM+E  NQ+LSL+GKIPSG FN MF    CWQ DA 
Sbjct: 64  NVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAA 123

Query: 127 STRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKM 186
            T++LAFDG FI+LY++ L+KS + L E VK+ VPS+WDPAALA FI+ YGTH++V VKM
Sbjct: 124 YTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKM 183

Query: 187 GGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDA---NGGLTSNPVAAKIKDEYSLP 246
           GGKDVI+ +Q   S ++  D+QK+LK++AD +F E +   N G      ++K++ +    
Sbjct: 184 GGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQ-- 243

Query: 247 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVD-VGQSHNQWLSTISQSPNVISMSFV 306
             LR A  +S+     +++  ++   +  RRGG D     HN+WL T+   P+VISMSF+
Sbjct: 244 -RLRFADTSSLG----SYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFI 303

Query: 307 PITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQ 366
           PITSL+NGV G+GFLSHA+NLYLRYKPPIEEL QFLEFQLPRQWAPV+++LPL P+ ++Q
Sbjct: 304 PITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQ 363

Query: 367 ASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLS 426
           +  SL+F+  GPKLYVNTT VD    P+TG+RL+LEG++S+ LAIHLQHLS+LPK  QL 
Sbjct: 364 SCASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLE 423

Query: 427 DDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGM 486
           DDL+     E  ++ Y+E V W  +SHVCT PVE       DD  S+VT A   V+  G 
Sbjct: 424 DDLNRSIRQESHDRRYYEKVNWKNYSHVCTEPVE------SDDDLSVVTGAQLHVESHGF 483

Query: 487 RKVLFLRLGFSMVASAG-IRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 546
           + VLFLRL FS V  A  ++ S+W+     + KSGLIST IS  F+A    P     + +
Sbjct: 484 KNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNI 543

Query: 547 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 606
           NSA+YP GPP+ ++A K+L  +DT EM RGP+ SPGYWVV+GA+L VE G+IS+KVKYSL
Sbjct: 544 NSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 600

Query: 607 LTILSEDSMI 611
            T +  D +I
Sbjct: 604 FTPILGDEVI 600

BLAST of CsaV3_1G012540 vs. Swiss-Prot
Match: sp|Q8L612|MACP1_ARATH (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 3.0e-129
Identity = 257/614 (41.86%), Postives = 376/614 (61.24%), Query Frame = 0

Query: 15  EKAVSVIGFGYDLCNDLRLSSCKPGPSGS---KLIDIDFTRSRDLVLPAGVVVPNVPTSI 74
           E AV  +G G+DL  D RL  CK G   +   +L+ +D T++R+L +P   V  NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 75  NCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLK-GCWQNDAGSTRSL 134
           NCDKGERTRFRSD++ FN+MSE  NQ+ S++GKIPSG FN  FG + G W  DA + +SL
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 135 AFDGCFITLYNIELEK-SHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKD 194
             D   +TL+N+ +   + + L+++V+  VPSSWDP  LA FIE+YGTHV+ GV +GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 195 VIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYSLPW--ELRN 254
           V+ +RQ + S+++   ++  L  L D  F+      L++  +       +S P   E  N
Sbjct: 190 VVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCL--LSTRRLNKAYHHSHSQPKFPEAFN 249

Query: 255 AF----AASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPI 314
            F      +     IN   G  +T I  +RGG    +SH++WL T+   P+ I+ +F+PI
Sbjct: 250 VFDDKQTVAFNNFSINSQNG--ITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPI 309

Query: 315 TSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPL-APRHRKQA 374
           TSL+  V G+G LSHA++LYLRYKPP+ +LQ FL+F  PR WAPV+ DLP  A  +   A
Sbjct: 310 TSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASA 369

Query: 375 SPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTL--QL 434
            P+L    MGPKLYVNTT V S   PVTG+R FLEGKK + LAIHLQHL     T+  ++
Sbjct: 370 YPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKI 429

Query: 435 SDDLSYRPIDE-PEEKGYFEPVKWSIFSHVCTAPVEYNGSLI--------DDDSASIVTK 494
           +D+  +R  D+  +   YFEP+    FSHVCT PV+Y+ + I         +D A IVT 
Sbjct: 430 TDEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTG 489

Query: 495 AWFEVKGIGMRKVLFLRLGFSMVASAGIRRSDW-EGPSTTSRKSGLISTFISTRFSAGLH 554
           A  EVK  G + VL LRL ++ V+   + ++ W  GP  TS+KSG+ S+      S  +H
Sbjct: 490 AQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVH 549

Query: 555 Q---PEEQPKIELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCV 602
                +++ ++ L+S V+P GPP+ +   K++  +D  ++ RGP++SPG+W+VTG +L +
Sbjct: 550 HNMIQKDKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTGVRLYL 609

BLAST of CsaV3_1G012540 vs. Swiss-Prot
Match: sp|Q9C7N2|CAD1_ARATH (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 1.2e-117
Identity = 238/600 (39.67%), Postives = 348/600 (58.00%), Query Frame = 0

Query: 17  AVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVVVPNVPTSINCDKG 76
           A+  +G G+D+ +D+RL  CK  P GS+L+ I+  ++RDL L  G ++PNVP  I+C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKGAP-GSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 77  ERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAGSTRSLAFDGCF 136
                R  V SF++M+E  N +  + G IP G FN MF   G WQ DA ST+SLA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 137 ITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKDVIFMRQL 196
           I LY+++L K  + L  +++R VPSSWDPA+LA FIE YGTH+V  V +GG+DV+++RQ 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 197 RESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYSLPWELRNAFAASIKPP 256
           + S +  ++++  +  +   +F E  +  +T  P+  K KD                   
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHEAESQSIT-GPLKYKDKD------------------- 263

Query: 257 VINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITSLMNGVRGNGFL 316
                    +T I+ RRGG D+ QSH +W  T+  +P++I+M+F PI SL+ GV G   L
Sbjct: 264 ---------ITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHL 323

Query: 317 SHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQASPSLKFTLMGPKLY 376
           + A+ LYL YKPPIE+LQ FL++Q+ R WAP  ++L    + ++    SL+F+LMGPKL+
Sbjct: 324 TRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLF 383

Query: 377 VNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDDLSYRPIDEPEEKG 436
           ++  +V     PVTG+RL LEG K + L+IHLQHL +LPK LQ   D S+ PI  P+ +G
Sbjct: 384 ISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWD-SHVPIGAPKWQG 443

Query: 437 -------YFEPVKWSIFSHVCTAPVEYNGSLIDD-DSASIVTKAWFEVKGIGMRKVLFLR 496
                  +FEP+KW  FSHV T+P+E+  + I D     IVT A   V   G + VL L+
Sbjct: 444 PEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLK 503

Query: 497 LGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELNSAVYPDG 556
           L FS V    IRRS W+                        H P     +  +  + P G
Sbjct: 504 LLFSKVPGCTIRRSVWD------------------------HTP-----VASSGRLEPGG 559

Query: 557 PPLTS-------RAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLL 602
           P  +S       ++ K+  ++D+ EM++GP++ PG+W+VTGAKL VE G+I ++VKYSLL
Sbjct: 564 PSTSSSTEEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559

BLAST of CsaV3_1G012540 vs. TrEMBL
Match: tr|A0A0A0LV66|A0A0A0LV66_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G075560 PE=4 SV=1)

HSP 1 Score: 1235.7 bits (3196), Expect = 0.0e+00
Identity = 610/610 (100.00%), Postives = 610/610 (100.00%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480
           SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of CsaV3_1G012540 vs. TrEMBL
Match: tr|A0A2N9FXM5|A0A2N9FXM5_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS23249 PE=4 SV=1)

HSP 1 Score: 929.5 bits (2401), Expect = 3.9e-267
Identity = 451/603 (74.79%), Postives = 522/603 (86.57%), Query Frame = 0

Query: 9   NPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVVVPNVP 68
           NP  AA KAV  IG GYDLC+D+RLS+CK GP GSKLI++D T++RD+V+P GVVVPNVP
Sbjct: 9   NPHFAAAKAVEAIGLGYDLCSDIRLSACKSGPLGSKLIELDQTQTRDIVIPGGVVVPNVP 68

Query: 69  TSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAGSTR 128
            SI CDKGERTRFRSDV+SF+QMSE  NQ++SLSGKIPSG FN MF  +GCWQ DA +TR
Sbjct: 69  NSIKCDKGERTRFRSDVVSFHQMSEQFNQEMSLSGKIPSGLFNAMFNFRGCWQMDAAATR 128

Query: 129 SLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGK 188
           SLAFDG FITLYNIEL++  ITLSE VK+EVPSSWDPAALAEFIEKYGTHV+VGVKMGGK
Sbjct: 129 SLAFDGWFITLYNIELDRPQITLSEHVKQEVPSSWDPAALAEFIEKYGTHVIVGVKMGGK 188

Query: 189 DVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNP--VAAKIKDEYSLPWELR 248
           DV+ ++QL  SN++ TDVQK LK+ AD++FSED NG    NP  ++ K+K+E+ +PW+LR
Sbjct: 189 DVVHIKQLENSNLQPTDVQKILKQFADERFSEDENGSFLPNPAELSRKMKEEHPMPWDLR 248

Query: 249 NAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITSL 308
           N+FAASI+PP I HSK D +T I IRRGGVDV QSHNQWLSTI QSPNVISMSFVPITSL
Sbjct: 249 NSFAASIRPPDITHSKNDIVT-ICIRRGGVDVCQSHNQWLSTIMQSPNVISMSFVPITSL 308

Query: 309 MNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQASPSL 368
           ++GVRGNGFL HAVNLYLRY+PPIEEL QFLEFQLPRQWAP+Y+DLPL  RHRKQ SPSL
Sbjct: 309 LSGVRGNGFLGHAVNLYLRYRPPIEELHQFLEFQLPRQWAPMYSDLPLGLRHRKQVSPSL 368

Query: 369 KFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDDLSY 428
           +F+LMGP+LYVNT +VDS N PVTGVRL+LEGKKSDHLAIHLQHLSTLPK LQL+DD  Y
Sbjct: 369 QFSLMGPRLYVNTMQVDSGNRPVTGVRLYLEGKKSDHLAIHLQHLSTLPKILQLADDHIY 428

Query: 429 RPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGMRKVLF 488
            PI+EP ++GYFE VKWSIFSHVCTAPV+YN + I  +SASIVTKAWFEVK +GMRKVLF
Sbjct: 429 EPIEEPVDRGYFEAVKWSIFSHVCTAPVQYNDTHI-GESASIVTKAWFEVKVMGMRKVLF 488

Query: 489 LRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELNSAVYP 548
           LRLG+SMVASA +RRS WEGPST+SRKSG IS  ISTRFSAGL+ PE+  K+++NSAVYP
Sbjct: 489 LRLGYSMVASAKLRRSAWEGPSTSSRKSGFISMMISTRFSAGLNPPEKPAKVDMNSAVYP 548

Query: 549 DGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLLTILSE 608
            GPPL ++A KM N ++TKEMV+GPE+ PGYWVVTGAKLCVEGG+ISIKVKYSLLTI+ E
Sbjct: 549 GGPPLPAKAPKMSNFVNTKEMVKGPEDPPGYWVVTGAKLCVEGGKISIKVKYSLLTIMLE 608

Query: 609 DSM 610
           DSM
Sbjct: 609 DSM 609

BLAST of CsaV3_1G012540 vs. TrEMBL
Match: tr|A0A2R6QLD0|A0A2R6QLD0_ACTCH (MACPF domain-containing protein OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc17314 PE=4 SV=1)

HSP 1 Score: 926.0 bits (2392), Expect = 4.3e-266
Identity = 440/612 (71.90%), Postives = 526/612 (85.95%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           M+F   RF+PQSAAEKAVSVIGFGYDL  D+RLS CKPGPSG  LI++D TR++DLV+P 
Sbjct: 1   MAFKATRFDPQSAAEKAVSVIGFGYDLSTDIRLSGCKPGPSGWGLIEVDRTRTKDLVVPG 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNV  SI CDKGERTRFRSDV+SFNQMSE  NQ++SLSGKIP G FN MF  +GCW
Sbjct: 61  GVVVPNVSASIKCDKGERTRFRSDVLSFNQMSEQFNQEVSLSGKIPCGLFNYMFSFRGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           Q DA STRSLAFDG FITLYNIEL KS I LSE VK EVP+SWDP  LAEFIEKYGTHVV
Sbjct: 121 QKDAASTRSLAFDGWFITLYNIELAKSQIILSEHVKNEVPASWDPVVLAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLT--SNPVAAKIKDE 240
           VGVKMGGKDVI ++QL+ SN++  +VQK LK+LAD++FSED NG  T  +N ++ K KDE
Sbjct: 181 VGVKMGGKDVIHIKQLQNSNLQPAEVQKLLKQLADERFSEDTNGSFTLDTNKLSGKRKDE 240

Query: 241 YSLPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISM 300
           +++ W+LR++F  S+ P V++HSK D++ +I++RRGG+D GQSH+ WLST+SQSPNVISM
Sbjct: 241 HNVTWDLRSSFMTSMVPSVVSHSKNDDILSIHVRRGGIDNGQSHSDWLSTVSQSPNVISM 300

Query: 301 SFVPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRH 360
           SF+PI SL++G+RG+GFLSHA+NLYLRYKPPIEEL QFLEFQLPRQWAPVY DLPLA RH
Sbjct: 301 SFIPIASLLSGIRGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLARRH 360

Query: 361 RKQASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTL 420
           ++Q SPSL+FT MGP+LY+NT+KVDS N PVTG+RL+LEGK+SDHLA+HLQHLSTLP++L
Sbjct: 361 KRQVSPSLQFTFMGPRLYINTSKVDSGNRPVTGIRLYLEGKRSDHLAVHLQHLSTLPQSL 420

Query: 421 QLSDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKG 480
           QLSDD SY PI+EP E+GY+EPVKWSIFSHVCTAPV+Y+G+ I D+SASIVTKAWFEVK 
Sbjct: 421 QLSDDPSYEPINEPIERGYYEPVKWSIFSHVCTAPVQYDGARI-DESASIVTKAWFEVKL 480

Query: 481 IGMRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKI 540
           +GM+KVLFLRLGFS VASA IRRS+W+GP+T+SRKSG +S  IST F+ G+   E+ PK+
Sbjct: 481 VGMKKVLFLRLGFSTVASARIRRSEWDGPTTSSRKSGFMSMLISTTFTTGMTPIEKPPKV 540

Query: 541 ELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKY 600
           +LNSAVYP GPP+  RA K+   +DTKEMVRGPE+SPGYWVVTGAKLCVEGGRI+IK KY
Sbjct: 541 DLNSAVYPGGPPVPKRAPKLTYFVDTKEMVRGPEDSPGYWVVTGAKLCVEGGRITIKAKY 600

Query: 601 SLLTILSEDSMI 611
           SLLTIL EDS++
Sbjct: 601 SLLTILLEDSLM 611

BLAST of CsaV3_1G012540 vs. TrEMBL
Match: tr|A0A2P5CJZ2|A0A2P5CJZ2_9ROSA (Membrane attack complex component/perforin (MACPF) domain containing protein OS=Trema orientalis OX=63057 GN=TorRG33x02_281930 PE=4 SV=1)

HSP 1 Score: 924.1 bits (2387), Expect = 1.7e-265
Identity = 446/607 (73.48%), Postives = 524/607 (86.33%), Query Frame = 0

Query: 6   NRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVVVP 65
           N   P+SAAEKAVSVIGFGYD+C D+RLSSCK G SGS+LI++D T+ RDLVLP GVVV 
Sbjct: 7   NGLTPRSAAEKAVSVIGFGYDVCRDIRLSSCKSGHSGSRLIELDPTQVRDLVLPGGVVVS 66

Query: 66  NVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAG 125
           +VPTSI CDKGERTRFRSDV+SFNQMSE  N++LSLSGKIP G FNT+FG KGCWQ DA 
Sbjct: 67  DVPTSIKCDKGERTRFRSDVLSFNQMSEQINKELSLSGKIPCGMFNTVFGFKGCWQKDAA 126

Query: 126 STRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKM 185
            T+SLAFDG FI+LYNIEL++++I LS+QVK+EVPS WDPAALAEFIEKYGTH+VVGVKM
Sbjct: 127 HTKSLAFDGWFISLYNIELQRTNIALSKQVKQEVPSLWDPAALAEFIEKYGTHIVVGVKM 186

Query: 186 GGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDA--NGGLTSNPVAAKIKDEYSLPW 245
           GGKDV+ ++QL+ S ++  +VQK LK+LAD++FSE+   +  L ++ ++ K K+E+S+  
Sbjct: 187 GGKDVVHVKQLQNSQLQPNEVQKLLKELADERFSENVKRSSALNAHELSGKTKEEHSMSL 246

Query: 246 ELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPI 305
           +  NAFAASI+PPVI HS  D++ +I +RRGG+++GQSHN WLST+SQSPNV+SMS VPI
Sbjct: 247 DHYNAFAASIRPPVITHSIKDDIMSICVRRGGIEIGQSHNSWLSTVSQSPNVVSMSLVPI 306

Query: 306 TSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQAS 365
           TSL+ GV G GFLSHAVNLYLRYKPPIEEL QFLEFQLPRQWAPVY DLPL  RHRK AS
Sbjct: 307 TSLLGGVPGYGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLTLRHRKHAS 366

Query: 366 PSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDD 425
           PSL+FT MGPKLYVNT KVDS   PVTG+RL+LEGKKSDHLA+HLQHLSTLP+ LQ+SDD
Sbjct: 367 PSLQFTFMGPKLYVNTMKVDSGGRPVTGLRLYLEGKKSDHLAVHLQHLSTLPENLQISDD 426

Query: 426 LSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGMRK 485
             Y PI+EP E+GYFEPVKWSIFSHVCT PV+YNG+ I D+SASIVTKAWFEVK IGMRK
Sbjct: 427 HGYEPIEEPIERGYFEPVKWSIFSHVCTVPVQYNGACI-DESASIVTKAWFEVKVIGMRK 486

Query: 486 VLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELNSA 545
           VLFLRLGFS VASA IRRS+WEGP+ +SRKSG +S  ISTRFS+GL+ P + PK++LNSA
Sbjct: 487 VLFLRLGFSSVASAKIRRSEWEGPANSSRKSGFLSVLISTRFSSGLNPPVKPPKVDLNSA 546

Query: 546 VYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLLTI 605
           VYPDGPPL ++A KM + IDTKEMVRGPE+SPGYWVVTGAKLCVEGGRISIKVKYSLLTI
Sbjct: 547 VYPDGPPLPAKAPKMASFIDTKEMVRGPEDSPGYWVVTGAKLCVEGGRISIKVKYSLLTI 606

Query: 606 LSEDSMI 611
           +SEDSM+
Sbjct: 607 MSEDSML 612

BLAST of CsaV3_1G012540 vs. TrEMBL
Match: tr|A0A2P5AMT8|A0A2P5AMT8_PARAD (Membrane attack complex component/perforin (MACPF) domain containing protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_316970 PE=4 SV=1)

HSP 1 Score: 921.8 bits (2381), Expect = 8.2e-265
Identity = 443/607 (72.98%), Postives = 521/607 (85.83%), Query Frame = 0

Query: 6   NRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVVVP 65
           N   P+SAAEKAVSVIGFGYD+C D+RLSSCK G SGS+LI++D T+  DLVLP GV+V 
Sbjct: 7   NGLTPRSAAEKAVSVIGFGYDVCKDIRLSSCKSGHSGSRLIELDPTQVGDLVLPGGVIVS 66

Query: 66  NVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAG 125
           +VPTSI CDKGERTRFRSDV+SFNQMSE  N++LSLSGKIP G FNT+FG KGCWQ DA 
Sbjct: 67  DVPTSIKCDKGERTRFRSDVLSFNQMSEQINKELSLSGKIPCGMFNTVFGFKGCWQKDAA 126

Query: 126 STRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKM 185
            T+SLAFDG FI+LYNIELE+++I LS+QVK+EVPS WDPAALAEFIEKYGTH+VVGVKM
Sbjct: 127 HTKSLAFDGWFISLYNIELERTNIALSKQVKQEVPSLWDPAALAEFIEKYGTHIVVGVKM 186

Query: 186 GGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDAN--GGLTSNPVAAKIKDEYSLPW 245
           GGKDV+ ++QL+ S+++  +VQK LK+LAD++FSE+ N    L ++ ++ K K E+S+P 
Sbjct: 187 GGKDVVHVKQLQNSHLQPNEVQKLLKELADERFSENVNRSSALNAHELSGKTKQEHSMPL 246

Query: 246 ELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPI 305
           +  NAFAASI+PPVI HS  D++ +I +RRGG+++GQSHN WLST+SQSPNV+SMS VPI
Sbjct: 247 DHYNAFAASIRPPVITHSIKDDIMSICVRRGGIEIGQSHNSWLSTVSQSPNVVSMSLVPI 306

Query: 306 TSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQAS 365
           TSL+ GV G GFLSHAVNLYLRYKPPIEEL QFLEFQLPRQWAP Y DLPL  RHRK A 
Sbjct: 307 TSLLGGVPGLGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPAYGDLPLTLRHRKHAY 366

Query: 366 PSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDD 425
           PSL+FT MGPKLYVNT KVDS   PVTG+RL+LEGKKSDHLA+HLQHLSTLP+ LQ+SDD
Sbjct: 367 PSLQFTFMGPKLYVNTMKVDSGGRPVTGLRLYLEGKKSDHLAVHLQHLSTLPENLQISDD 426

Query: 426 LSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGMRK 485
             Y PIDEP E+GYFEPVKWSIFSHVCT PV+YNG+ I D+SASIVTKAWFE+K IGMRK
Sbjct: 427 HGYEPIDEPIERGYFEPVKWSIFSHVCTVPVQYNGACI-DESASIVTKAWFEIKVIGMRK 486

Query: 486 VLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELNSA 545
           VLFLRLGFS VASA IRRS+WEGP+ +SRKSG +S  IS RFS+GL+ PE+ PK++LNSA
Sbjct: 487 VLFLRLGFSSVASAKIRRSEWEGPANSSRKSGFLSVLISARFSSGLNPPEKPPKVDLNSA 546

Query: 546 VYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLLTI 605
           VYPDGPPL ++A KM  +IDTKEMVRGPE+ PGYWVVTGAKLC+EGGRISIKVKYSLLTI
Sbjct: 547 VYPDGPPLPAKAPKMARLIDTKEMVRGPEDPPGYWVVTGAKLCLEGGRISIKVKYSLLTI 606

Query: 606 LSEDSMI 611
           +SEDSM+
Sbjct: 607 MSEDSML 612

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004146657.10.0e+00100.00PREDICTED: MACPF domain-containing protein NSL1 [Cucumis sativus] >KGN64702.1 hy... [more]
XP_022994356.10.0e+0090.98MACPF domain-containing protein NSL1 [Cucurbita maxima][more]
XP_023542863.10.0e+0090.49MACPF domain-containing protein NSL1 [Cucurbita pepo subsp. pepo][more]
XP_022955164.10.0e+0090.49MACPF domain-containing protein NSL1 isoform X2 [Cucurbita moschata][more]
XP_022140066.10.0e+0088.89MACPF domain-containing protein NSL1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G28380.13.9e-22863.73MAC/Perforin domain-containing protein[more]
AT4G24290.22.0e-17952.13MAC/Perforin domain-containing protein[more]
AT1G14780.11.7e-13041.86MAC/Perforin domain-containing protein[more]
AT1G29690.16.6e-11939.67MAC/Perforin domain-containing protein[more]
Match NameE-valueIdentityDescription
sp|Q9SGN6|NSL1_ARATH7.0e-22763.73MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=... [more]
sp|Q9STW5|MACP2_ARATH3.5e-17852.13MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4... [more]
sp|Q8L612|MACP1_ARATH3.0e-12941.86MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
sp|Q9C7N2|CAD1_ARATH1.2e-11739.67MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0LV66|A0A0A0LV66_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G075560 PE=4 SV=1[more]
tr|A0A2N9FXM5|A0A2N9FXM5_FAGSY3.9e-26774.79Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS23249 PE=4 SV=1[more]
tr|A0A2R6QLD0|A0A2R6QLD0_ACTCH4.3e-26671.90MACPF domain-containing protein OS=Actinidia chinensis var. chinensis OX=1590841... [more]
tr|A0A2P5CJZ2|A0A2P5CJZ2_9ROSA1.7e-26573.48Membrane attack complex component/perforin (MACPF) domain containing protein OS=... [more]
tr|A0A2P5AMT8|A0A2P5AMT8_PARAD8.2e-26572.98Membrane attack complex component/perforin (MACPF) domain containing protein OS=... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR020864MACPF
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0006862 nucleotide transport
biological_process GO:0009862 systemic acquired resistance, salicylic acid mediated signaling pathway
biological_process GO:0009697 salicylic acid biosynthetic process
biological_process GO:0009651 response to salt stress
biological_process GO:0034976 response to endoplasmic reticulum stress
biological_process GO:0010200 response to chitin
biological_process GO:0002679 respiratory burst involved in defense response
biological_process GO:0010337 regulation of salicylic acid metabolic process
biological_process GO:0010363 regulation of plant-type hypersensitive response
biological_process GO:0043900 regulation of multi-organism process
biological_process GO:0043269 regulation of ion transport
biological_process GO:0010310 regulation of hydrogen peroxide metabolic process
biological_process GO:0006612 protein targeting to membrane
biological_process GO:0043069 negative regulation of programmed cell death
biological_process GO:0031348 negative regulation of defense response
biological_process GO:0000165 MAPK cascade
biological_process GO:0009867 jasmonic acid mediated signaling pathway
biological_process GO:0006888 ER to Golgi vesicle-mediated transport
biological_process GO:0006984 ER-nucleus signaling pathway
biological_process GO:0009595 detection of biotic stimulus
biological_process GO:0050832 defense response to fungus
biological_process GO:0042742 defense response to bacterium
biological_process GO:0052542 defense response by callose deposition
biological_process GO:0015802 basic amino acid transport
biological_process GO:0015696 ammonium transport
biological_process GO:0043090 amino acid import
cellular_component GO:0005575 cellular_component
cellular_component GO:0005737 cytoplasm
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_1G012540.1CsaV3_1G012540.1mRNA


Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020864Membrane attack complex component/perforin (MACPF) domainSMARTSM00457MACPF_8coord: 127..325
e-value: 2.6E-10
score: 50.3
IPR020864Membrane attack complex component/perforin (MACPF) domainPFAMPF01823MACPFcoord: 110..323
e-value: 4.4E-32
score: 111.8
IPR020864Membrane attack complex component/perforin (MACPF) domainPROSITEPS51412MACPF_2coord: 2..337
score: 23.011
NoneNo IPR availablePANTHERPTHR33199FAMILY NOT NAMEDcoord: 4..609
NoneNo IPR availablePANTHERPTHR33199:SF8MACPF DOMAIN-CONTAINING PROTEIN NSL1coord: 4..609

The following gene(s) are paralogous to this gene:

None