CSPI01G12360 (gene) Wild cucumber (PI 183967)

NameCSPI01G12360
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionMACPF domain-containing protein
LocationChr1 : 7813360 .. 7818141 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATGGTTGGAGAAACAAGAAATAATAAATAATTCATGGAGTAGAGCGTTGAAGAGGAGATGAAAAGATAATGAAAATATGAAATTGGGCAGTTGGAGATTTGAAAGTGAAACGCAGCAGACGCAAGACGAAGAATAAACAATTGTCTGAATTTTCTAATCAGAAGAAGAAGAAGAAAAAGAATGGGTTGTTGGGTAAAGAAATTTTCAGAGAGTCGACCCTTCGGATCCACCATCTCCACCTCCTACGTGAACTTTCTACAATGGCGGATCTTACTGTATTTTCTAAAGGCCGTACGCGTATTGACTTTTCAGCCGCCATCTACAGCTCCTTCTTCTTCATCTGCTTCTTCTTCGCCGAATCCAATAATTACTCTGGCAAGTCAAACTTTGGTTAACTCAGTCTTCATCCCAATTTCCATATCCAATTAAACAATTCATAAGTGCCTCTCCCACCGTATTTTCTCTCTCCCCTTTTTTGTATTATATCTTTTGAATTTTTCTCTTCCCACATTTTCTTCGGTCAAAACTTAGAACTCTGCAATACGCTTTGAAAACAGCAACCCCCACTTTCAATCTTTTCAACGCCATACTTTTGGGTTTTCTTCTTCTTTGTAGTCGTCGCCTTTTTCCCTTTCCATCAAAACCCTAAAGGGATTTCATCCAATTCGACGGAATTTTGGTGTTTGTCCGTTGAGAAATGTCTTTCAACATCAACCGGTTCAATCCTCAATCAGCCGCCGAGAAAGCGGTTTCAGTTATAGGCTTCGGCTACGATCTCTGTAACGATTTGCGATTGTCTTCTTGCAAGCCGGGTCCATCCGGGTCTAAACTGATCGATATTGATTTCACTCGAAGTAGAGACCTTGTACTTCCGGCCGGCGTTGTCGTCCCTAATGTTCCTACTTCCATCAACTGTGATAAAGGTGAGCGTACCCGATTCCGGTCGGATGTCATCTCCTTCAATCAGGTAATTTTCTTTGAAATTAGTTGTTGCTGATTTTATAGCTTAGAATTCTCTTCGTTTTATTGTTTTTTTTTTTTTTTTTTTTTTTTTTTCATTTGTTGGCTTCATAAAACGAACTGGGTTTGTGTTTTTTCCGGTTAAAAACAGATGTCGGAGCTGTGTAATCAGCAATTATCTCTATCTGGGAAAATTCCGTCGGGGTTCTTTAACACCATGTTTGGATTAAAGGGTTGCTGGCAAAACGATGCAGGTTCCACAAGAAGTCTTGCATTTGATGGTTGTTTCATTACACTTTATAACATTGAGTTGGAAAAATCTCATATAACTCTCTCTGAGCAAGTCAAAAGGGAGGTTCCTTCTTCATGGGATCCTGCTGCCCTTGCAGAGTATGTTTCTTTTGTTTTTTGTTTTTATTTTTGTTTCCATTTTGTCTTTTCTTTTGTAGAAATCTAGACATTTGGATGTTTGTCTGTCCTCTTCTATTGAAATATCTTCATCACTGATATTTAACAAACTTATATTTGTTCATCTTCTCCACATCAACACACTGATTGTTAATGCTGTTGAATGGATGAGAATGGATTTTTCGAAGTAATGTTTTCAACCATGCGCTTCTCTGTCCAACACTTGAGGAAGGACTGGTCTTTGAAATTGACTTGACTATGTTGCCAAAAAGGTGGAAAATGTGCATTTAGTTAGCTACTTTTATGTTTTACTATTCCAAAAGTAAATAAAAACAGTGATGGATGAGTACCTTTCTTGAAAGTTGATGGCTTGGTATGAACCTATTCTTTTAAGTCCTTCGCTTTTTCCCCTTTGTCCTAACAATCACTAGGTCATTTCATGGTCGTCGGTTCTACTGAACTTCCTAACTGTCAGCTTAAAGGATGGTCCAATTAGTATAATCCCTTAGATTCATCTAATATATCATACAAGACACGTTTTGTTCACGAGTTCTTTGGTTTAGATTACCTAAGTGTTTGTTGGGTACGTCTCATAATTACCTATATGTGTTTTTGTTGCATTTGAAACATTGTTTATCGAAGACATTACACAAACACTATCACGTTAGACATTAGGGACATATCAAATACTTGTTAAAGTGAAACACATTGGATATCTGATTTTTGTGCCAACTAAATACTAACTTGATTTCAGGTTCATTGAGAAATATGGTACTCATGTGGTTGTGGGTGTAAAAATGGGAGGAAAAGATGTCATTTTCATGAGGCAATTACGAGAATCGAATGTAGAGCAAACCGATGTGCAGAAGAAGTTAAAGAAATTAGCAGATGATAAATTTTCTGAAGATGCAAATGGAGGTTTAACGTCCAATCCTGTTGCAGCAAAAATCAAGGTACACACTCGAGAACATAAATGGCTATCACCATTTGGTCTATTCCATTGGTCCCATGTCAAAATAGAGGATGTTGATTAGCTATTGACGGAAATTAAAGAATCAATTTTCCTTGAGTCATGCATTGTATGCAGCTCTAGATTCATGTTCTTTCCTTAAAATTCTTAGATTGAGATAAAATCATGTCAATAGAATATGGTTTAGTTGCTAAGTTCCGTATAGAATGTGGTTTAATTGTTACCTGATATAGCAGTTATCATGCCTTTATCATCAGCTTGGCTTGCTTGTGAATTCTTCTTCTACTTCTGCATCTACACATATACTAAATATAACTCAACTAAGATTTATAAAAACTCCATAAACATGGCTAGTCAGATAATAATCTTTGCTAATTGTATTTAAATTAATCATTATAACCATGGGGCATACAATTTCTGTTCCAATAGATTATTCTTTTTTTAAAACAATTTTGATAGGATAAATGATTCGCAATGTCATATTATTCACATAGAACAGAAAATTTAAAAGTGCAAACAAGTTTCGTGGTTTAATGATGCATCTACTAACGACATTTTCTATATGTATAAACATTTTAAGTCTCATCAGGAGAAATAAACTTTTATCCTCGATGATTTCAATTTTTAGGATGAATATTCTCTGCCATGGGAGCTTCGTAATGCATTTGCTGCCTCTATCAAACCACCAGTGATCAATCACTCAAAGGGTGATGTAAGCATCTCGGCATCTACCTGTTCGTTCGAGGAGAATATTGCTACACTTTCGACCTATTAAGCAGATGAGAACTGACTTATTTAATTAAACAACTGGATGCAGGAATTAACAGCGATTTATATTAGAAGAGGAGGTGTTGATGTTGGTCAAAGTCATAATCAATGGCTCTCAACCATTTCTCAGTCACCTAATGTTATCTCAATGTCCTTTGTACCAATAACATCACTGATGAATGGCGTCCGAGGCAATGGATTTCTAAGCCATGCCGTAAACCTGTATCTAAGATGTAAGTTGCAAGTCTTGCTTTTAAGTGTTGTTTCTCTACAGCGAATGGACCTGGATAATTTTTGTCCTTTAACGAGAACCTGAAATATGGAGATCAGATACGTACATTGTGTGTCCTTAAAATTTGACCTTAGTTACTCTTCCCACGGTGGCTGTAGTACTTGAAGCTTCCATTAAAAGATTTTGACCCAAGGTTCATGCCAACAAGGTACCGTTCTTATTAAGGTTAGTGTTAGATTTTGATCATTGAAAAATTGTGCAGATAAACCTCCAATTGAGGAGCTCCAGCAATTTCTTGAATTTCAACTGCCTAGACAGTGGGCGCCGGTGTACGCTGATCTTCCTCTTGCTCCTCGGCATAGGAAACAAGCCTCTCCATCTCTCAAGTTTACTTTGATGGGGCCCAAGCTTTATGTAAATACTACTAAGGTAATCGTCTGACTTGAATATTTGGGTCAGTCAAAAACTTCAAATAGCAATGGCAATGGCAATGTCGCAAATGTGATCACTGCTTGATTTTTGCCTGACCTATCTATTACCTTTGCTTGGATGCAAATTGTTGAAAATTTCACATGGTGTCACAATTTTGAAACTGCAGGTTGATTCAAACAATCTTCCAGTAACGGGAGTTCGCTTGTTTTTGGAAGGAAAGAAAAGTGACCATCTGGCAATTCATCTCCAGCATCTATCAACTCTTCCCAAGACTCTTCAACTCTCAGATGATCTAAGCTATCGACCCATCGACGAGCCAGAGGAGAAGGGCTACTTCGAACCAGTTAAATGGAGCATATTCTCTCATGTCTGCACTGCTCCAGTAGAGTACAATGGTTCTCTAATTGATGATGACTCTGCTTCTATCGTCACAAAGGCATGGTTTGAAGTTAAGGGTATTGGAATGAGAAAGGTCTTGTTCCTGAGGCTCGGGTTTTCGATGGTGGCATCAGCAGGGATTCGTCGATCGGACTGGGAAGGACCTTCAACAACATCCCGAAAATCTGGACTCATCTCCACCTTTATCAGCACAAGGTTTAGTGCAGGGTTGCATCAACCAGAAGAGCAACCAAAAATTGAATTGAATTCGGCTGTTTATCCTGATGGTCCACCATTGACTTCTAGGGCAATGAAAATGTTGAATGTCATCGATACGAAAGAAATGGTGCGTGGTCCTGAGAATTCACCTGGATATTGGGTGGTTACCGGAGCCAAGCTGTGCGTGGAAGGTGGCCGGATCTCTATCAAAGTGAAATACTCTTTGTTAACCATATTGTCGGAGGATTCTATGATCTGACAAACAGAAAACTTACGCAATGAGTAGATACAACTATTTTTATGTTGTAAATGTGTTTTGTACATTAGAGAAACACCATCATTCTTCTTTCACAGTGATACAAATAGTATTTTGAGAATGTAAATCTCTGCTGTATTATGCATTTTTTTGAACG

mRNA sequence

ATGTCTTTCAACATCAACCGGTTCAATCCTCAATCAGCCGCCGAGAAAGCGGTTTCAGTTATAGGCTTCGGCTACGATCTCTGTAACGATTTGCGATTGTCTTCTTGCAAGCCGGGTCCATCCGGGTCTAAACTGATCGATATTGATTTCACTCGAAGTAGAGACCTTGTACTTCCGGCCGGCGTTGTCGTCCCTAATGTTCCTACTTCCATCAACTGTGATAAAGGTGAGCGTACCCGATTCCGGTCGGATGTCATCTCCTTCAATCAGATGTCGGAGCTGTGTAATCAGCAATTATCTCTATCTGGGAAAATTCCGTCGGGGTTCTTTAACACCATGTTTGGATTAAAGGGTTGCTGGCAAAACGATGCAGGTTCCACAAGAAGTCTTGCATTTGATGGTTGTTTCATTACACTTTATAACATTGAGTTGGAAAAATCTCATATAACTCTCTCTGAGCAAGTCAAAAGGGAGGTTCCTTCTTCATGGGATCCTGCTGCCCTTGCAGAGTTCATTGAGAAATATGGTACTCATGTGGTTGTGGGTGTAAAAATGGGAGGAAAAGATGTCATTTTCATGAGGCAATTACGAGAATCGAATGTAGAGCAAACCGATGTGCAGAAGAAGTTAAAGAAATTAGCAGATGATAAATTTTCTGAAGATGCAAATGGAGGTTTAACGTCCAATCCTGTTGCAGCAAAAATCAAGGATGAATATTCTCTGCCATGGGAGCTTCGTAATGCATTTGCTGCCTCTATCAAACCACCAGTGATCAATCACTCAAAGGGTGATGAATTAACAGCGATTTATATTAGAAGAGGAGGTGTTGATGTTGGTCAAAGTCATAATCAATGGCTCTCAACCATTTCTCAGTCACCTAATGTTATCTCAATGTCCTTTGTACCAATAACATCACTGATGAATGGCGTCCGAGGCAATGGATTTCTAAGCCATGCCGTAAACCTGTATCTAAGATATAAACCTCCAATTGAGGAGCTCCAGCAATTTCTTGAATTTCAACTGCCTAGACAGTGGGCGCCGGTGTACGCTGATCTTCCTCTTGCTCCTCGGCATAGGAAACAAGCCTCTCCATCTCTCAAGTTTACTTTGATGGGGCCCAAGCTTTATGTAAATACTACTAAGGTTGATTCAAACAATCTTCCAGTAACGGGAGTTCGCTTGTTTTTGGAAGGAAAGAAAAGTGACCATCTGGCAATTCATCTCCAGCATCTATCAACTCTTCCCAAGACTCTTCAACTCTCAGATGATCTAAGCTATCGACCCATCGACGAGCCAGAGGAGAAGGGCTACTTCGAACCAGTTAAATGGAGCATATTCTCTCATGTCTGCACTGCTCCAGTAGAGTACAATGGTTCTCTAATTGATGATGACTCTGCTTCTATCGTCACAAAGGCATGGTTTGAAGTTAAGGGTATTGGAATGAGAAAGGTCTTGTTCCTGAGGCTCGGGTTTTCGATGGTGGCATCAGCAGGGATTCGTCGATCGGACTGGGAAGGACCTTCAACAACATCCCGAAAATCTGGACTCATCTCCACCTTTATCAGCACAAGGTTTAGTGCAGGGTTGCATCAACCAGAAGAGCAACCAAAAATTGAATTGAATTCGGCTGTTTATCCTGATGGTCCACCATTGACTTCTAGGGCAATGAAAATGTTGAATGTCATCGATACGAAAGAAATGGTGCGTGGTCCTGAGAATTCACCTGGATATTGGGTGGTTACCGGAGCCAAGCTGTGCGTGGAAGGTGGCCGGATCTCTATCAAAGTGAAATACTCTTTGTTAACCATATTGTCGGAGGATTCTATGATCTGA

Coding sequence (CDS)

ATGTCTTTCAACATCAACCGGTTCAATCCTCAATCAGCCGCCGAGAAAGCGGTTTCAGTTATAGGCTTCGGCTACGATCTCTGTAACGATTTGCGATTGTCTTCTTGCAAGCCGGGTCCATCCGGGTCTAAACTGATCGATATTGATTTCACTCGAAGTAGAGACCTTGTACTTCCGGCCGGCGTTGTCGTCCCTAATGTTCCTACTTCCATCAACTGTGATAAAGGTGAGCGTACCCGATTCCGGTCGGATGTCATCTCCTTCAATCAGATGTCGGAGCTGTGTAATCAGCAATTATCTCTATCTGGGAAAATTCCGTCGGGGTTCTTTAACACCATGTTTGGATTAAAGGGTTGCTGGCAAAACGATGCAGGTTCCACAAGAAGTCTTGCATTTGATGGTTGTTTCATTACACTTTATAACATTGAGTTGGAAAAATCTCATATAACTCTCTCTGAGCAAGTCAAAAGGGAGGTTCCTTCTTCATGGGATCCTGCTGCCCTTGCAGAGTTCATTGAGAAATATGGTACTCATGTGGTTGTGGGTGTAAAAATGGGAGGAAAAGATGTCATTTTCATGAGGCAATTACGAGAATCGAATGTAGAGCAAACCGATGTGCAGAAGAAGTTAAAGAAATTAGCAGATGATAAATTTTCTGAAGATGCAAATGGAGGTTTAACGTCCAATCCTGTTGCAGCAAAAATCAAGGATGAATATTCTCTGCCATGGGAGCTTCGTAATGCATTTGCTGCCTCTATCAAACCACCAGTGATCAATCACTCAAAGGGTGATGAATTAACAGCGATTTATATTAGAAGAGGAGGTGTTGATGTTGGTCAAAGTCATAATCAATGGCTCTCAACCATTTCTCAGTCACCTAATGTTATCTCAATGTCCTTTGTACCAATAACATCACTGATGAATGGCGTCCGAGGCAATGGATTTCTAAGCCATGCCGTAAACCTGTATCTAAGATATAAACCTCCAATTGAGGAGCTCCAGCAATTTCTTGAATTTCAACTGCCTAGACAGTGGGCGCCGGTGTACGCTGATCTTCCTCTTGCTCCTCGGCATAGGAAACAAGCCTCTCCATCTCTCAAGTTTACTTTGATGGGGCCCAAGCTTTATGTAAATACTACTAAGGTTGATTCAAACAATCTTCCAGTAACGGGAGTTCGCTTGTTTTTGGAAGGAAAGAAAAGTGACCATCTGGCAATTCATCTCCAGCATCTATCAACTCTTCCCAAGACTCTTCAACTCTCAGATGATCTAAGCTATCGACCCATCGACGAGCCAGAGGAGAAGGGCTACTTCGAACCAGTTAAATGGAGCATATTCTCTCATGTCTGCACTGCTCCAGTAGAGTACAATGGTTCTCTAATTGATGATGACTCTGCTTCTATCGTCACAAAGGCATGGTTTGAAGTTAAGGGTATTGGAATGAGAAAGGTCTTGTTCCTGAGGCTCGGGTTTTCGATGGTGGCATCAGCAGGGATTCGTCGATCGGACTGGGAAGGACCTTCAACAACATCCCGAAAATCTGGACTCATCTCCACCTTTATCAGCACAAGGTTTAGTGCAGGGTTGCATCAACCAGAAGAGCAACCAAAAATTGAATTGAATTCGGCTGTTTATCCTGATGGTCCACCATTGACTTCTAGGGCAATGAAAATGTTGAATGTCATCGATACGAAAGAAATGGTGCGTGGTCCTGAGAATTCACCTGGATATTGGGTGGTTACCGGAGCCAAGCTGTGCGTGGAAGGTGGCCGGATCTCTATCAAAGTGAAATACTCTTTGTTAACCATATTGTCGGAGGATTCTATGATCTGA
BLAST of CSPI01G12360 vs. Swiss-Prot
Match: NSL1_ARATH (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana GN=NSL1 PE=2 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 6.9e-227
Identity = 390/612 (63.73%), Postives = 474/612 (77.45%), Query Frame = 1

Query: 4   NINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVV 63
           N  R +  SAAEKAVSVIG GYDLC+D+R S+CK  P GS+L++ID TR+RDL+ P G+V
Sbjct: 5   NFTRLDAHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIV 64

Query: 64  VPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQND 123
           V NV +SI CDKGERTR RSD++SFNQMSE  NQ + LSGKIPSG FN MF    CW  D
Sbjct: 65  VNNVSSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKD 124

Query: 124 AGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGV 183
           A S ++LA+DG FI+LY++E+ +  +TL ++VKREVPSSWD AALA FIEKYGTHVVVGV
Sbjct: 125 ASSVKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGV 184

Query: 184 KMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSED-ANGGLTSNPVAAKIKDEYSLP 243
            MGGKDVI ++Q+R+SN E  ++QK LK   D++F  D       ++  + K K+E  L 
Sbjct: 185 TMGGKDVIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQ 244

Query: 244 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVP 303
           W L+  F  S+   V+ H+K +E+  + IRRGGVD+GQSH +WLST+SQ+PNVISM FVP
Sbjct: 245 WGLQ-PFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVP 304

Query: 304 ITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQA 363
           ITSL++G+ G GFLSHAVNLYLRYKPPIEEL QFLEFQLPRQWAPVY DLPL  R  KQ+
Sbjct: 305 ITSLLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQS 364

Query: 364 SPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSD 423
           SPSL+F+LMGPKLYVNT+KVDS   PVTG+R FLEGKK +HLAIHLQHLS  P +L LS 
Sbjct: 365 SPSLQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSH 424

Query: 424 DLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGMR 483
           D +Y PI+EP EKGY+ PVKW IFSHVCT PV+YNG+   DD+ASIVTKAW EVKG+GMR
Sbjct: 425 DDTYEPIEEPVEKGYYVPVKWGIFSHVCTYPVQYNGAR-SDDTASIVTKAWLEVKGMGMR 484

Query: 484 KVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLH----QPEEQPKI 543
           KVLFLRLGFS+ ASA  R+S W+  ST SRKSG+ S  ISTR S GL       + Q KI
Sbjct: 485 KVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKI 544

Query: 544 ELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKY 603
           ++NSAVYP GP    +  K+L+++DTKE++RGPE  PGYWVVTGAKLCVE G+ISIK KY
Sbjct: 545 DINSAVYPRGPSPPVKP-KLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKY 604

Query: 604 SLLTILSEDSMI 611
           SLLT++SEDS++
Sbjct: 605 SLLTVISEDSLV 612

BLAST of CSPI01G12360 vs. Swiss-Prot
Match: MACP2_ARATH (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 3.5e-178
Identity = 318/610 (52.13%), Postives = 419/610 (68.69%), Query Frame = 1

Query: 7   RFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDI-DFTRSRDLVLPAGVVVP 66
           R     AAE A+  IG GYDL  DLRL  CK G   S+L+DI +   + ++VLP G+ +P
Sbjct: 4   RLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIP 63

Query: 67  NVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAG 126
           NV  SI CDKGER RFRSD++ F QM+E  NQ+LSL+GKIPSG FN MF    CWQ DA 
Sbjct: 64  NVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAA 123

Query: 127 STRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKM 186
            T++LAFDG FI+LY++ L+KS + L E VK+ VPS+WDPAALA FI+ YGTH++V VKM
Sbjct: 124 YTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKM 183

Query: 187 GGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDA---NGGLTSNPVAAKIKDEYSLP 246
           GGKDVI+ +Q   S ++  D+QK+LK++AD +F E +   N G      ++K++ +    
Sbjct: 184 GGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQ-- 243

Query: 247 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQ-SHNQWLSTISQSPNVISMSFV 306
             LR A  +S+     +++  ++   +  RRGG D     HN+WL T+   P+VISMSF+
Sbjct: 244 -RLRFADTSSLG----SYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFI 303

Query: 307 PITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQ 366
           PITSL+NGV G+GFLSHA+NLYLRYKPPIEEL QFLEFQLPRQWAPV+++LPL P+ ++Q
Sbjct: 304 PITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQ 363

Query: 367 ASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLS 426
           +  SL+F+  GPKLYVNTT VD    P+TG+RL+LEG++S+ LAIHLQHLS+LPK  QL 
Sbjct: 364 SCASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLE 423

Query: 427 DDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGM 486
           DDL+     E  ++ Y+E V W  +SHVCT PVE       DD  S+VT A   V+  G 
Sbjct: 424 DDLNRSIRQESHDRRYYEKVNWKNYSHVCTEPVE------SDDDLSVVTGAQLHVESHGF 483

Query: 487 RKVLFLRLGFSMVASAG-IRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 546
           + VLFLRL FS V  A  ++ S+W+     + KSGLIST IS  F+A    P     + +
Sbjct: 484 KNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNI 543

Query: 547 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 606
           NSA+YP GPP+ ++A K+L  +DT EM RGP+ SPGYWVV+GA+L VE G+IS+KVKYSL
Sbjct: 544 NSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 600

Query: 607 LTILSEDSMI 611
            T +  D +I
Sbjct: 604 FTPILGDEVI 600

BLAST of CSPI01G12360 vs. Swiss-Prot
Match: MACP1_ARATH (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 2.3e-129
Identity = 256/612 (41.83%), Postives = 375/612 (61.27%), Query Frame = 1

Query: 15  EKAVSVIGFGYDLCNDLRLSSCKPGPSGS---KLIDIDFTRSRDLVLPAGVVVPNVPTSI 74
           E AV  +G G+DL  D RL  CK G   +   +L+ +D T++R+L +P   V  NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 75  NCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLK-GCWQNDAGSTRSL 134
           NCDKGERTRFRSD++ FN+MSE  NQ+ S++GKIPSG FN  FG + G W  DA + +SL
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 135 AFDGCFITLYNIELEK-SHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKD 194
             D   +TL+N+ +   + + L+++V+  VPSSWDP  LA FIE+YGTHV+ GV +GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 195 VIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYSLPW--ELRN 254
           V+ +RQ + S+++   ++  L  L D  F+      L++  +       +S P   E  N
Sbjct: 190 VVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCL--LSTRRLNKAYHHSHSQPKFPEAFN 249

Query: 255 AFAASIKPPVINHSKGDE--LTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITS 314
            F         N S   +  +T I  +RGG    +SH++WL T+   P+ I+ +F+PITS
Sbjct: 250 VFDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITS 309

Query: 315 LMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPL-APRHRKQASP 374
           L+  V G+G LSHA++LYLRYKPP+ +LQ FL+F  PR WAPV+ DLP  A  +   A P
Sbjct: 310 LLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYP 369

Query: 375 SLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTL--QLSD 434
           +L    MGPKLYVNTT V S   PVTG+R FLEGKK + LAIHLQHL     T+  +++D
Sbjct: 370 ALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITD 429

Query: 435 DLSYRPIDE-PEEKGYFEPVKWSIFSHVCTAPVEYNGSLI--------DDDSASIVTKAW 494
           +  +R  D+  +   YFEP+    FSHVCT PV+Y+ + I         +D A IVT A 
Sbjct: 430 EHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTGAQ 489

Query: 495 FEVKGIGMRKVLFLRLGFSMVASAGIRRSDW-EGPSTTSRKSGLISTFISTRFSAGLHQ- 554
            EVK  G + VL LRL ++ V+   + ++ W  GP  TS+KSG+ S+      S  +H  
Sbjct: 490 LEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVHHN 549

Query: 555 --PEEQPKIELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEG 602
              +++ ++ L+S V+P GPP+ +   K++  +D  ++ RGP++SPG+W+VTG +L ++ 
Sbjct: 550 MIQKDKNEVVLDSGVFPGGPPVPANN-KIVKFVDLSQLCRGPQHSPGHWLVTGVRLYLDK 609

BLAST of CSPI01G12360 vs. Swiss-Prot
Match: CAD1_ARATH (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana GN=CAD1 PE=2 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 1.2e-117
Identity = 239/600 (39.83%), Postives = 349/600 (58.17%), Query Frame = 1

Query: 17  AVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVVVPNVPTSINCDKG 76
           A+  +G G+D+ +D+RL  CK  P GS+L+ I+  ++RDL L  G ++PNVP  I+C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKGAP-GSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 77  ERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAGSTRSLAFDGCF 136
                R  V SF++M+E  N +  + G IP G FN MF   G WQ DA ST+SLA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 137 ITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKDVIFMRQL 196
           I LY+++L K  + L  +++R VPSSWDPA+LA FIE YGTH+V  V +GG+DV+++RQ 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 197 RESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYSLPWELRNAFAASIKPP 256
           + S +  ++++  +  +   +F E  +  +T  P+  K KD                   
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHEAESQSITG-PLKYKDKD------------------- 263

Query: 257 VINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITSLMNGVRGNGFL 316
                    +T I+ RRGG D+ QSH +W  T+  +P++I+M+F PI SL+ GV G   L
Sbjct: 264 ---------ITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHL 323

Query: 317 SHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQASPSLKFTLMGPKLY 376
           + A+ LYL YKPPIE+LQ FL++Q+ R WAP  ++L    + ++    SL+F+LMGPKL+
Sbjct: 324 TRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLF 383

Query: 377 VNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDDLSYRPIDEPEEKG 436
           ++  +V     PVTG+RL LEG K + L+IHLQHL +LPK LQ   D S+ PI  P+ +G
Sbjct: 384 ISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWD-SHVPIGAPKWQG 443

Query: 437 -------YFEPVKWSIFSHVCTAPVEYNGSLIDDDSA-SIVTKAWFEVKGIGMRKVLFLR 496
                  +FEP+KW  FSHV T+P+E+  + I D S   IVT A   V   G + VL L+
Sbjct: 444 PEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLK 503

Query: 497 LGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELNSAVYPDG 556
           L FS V    IRRS W+                        H P     +  +  + P G
Sbjct: 504 LLFSKVPGCTIRRSVWD------------------------HTP-----VASSGRLEPGG 559

Query: 557 PPLTS-------RAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLL 602
           P  +S       ++ K+  ++D+ EM++GP++ PG+W+VTGAKL VE G+I ++VKYSLL
Sbjct: 564 PSTSSSTEEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559

BLAST of CSPI01G12360 vs. TrEMBL
Match: A0A0A0LV66_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G075560 PE=4 SV=1)

HSP 1 Score: 1235.7 bits (3196), Expect = 0.0e+00
Identity = 610/610 (100.00%), Postives = 610/610 (100.00%), Query Frame = 1

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480
           SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of CSPI01G12360 vs. TrEMBL
Match: B9H2R6_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0004s04730g PE=4 SV=2)

HSP 1 Score: 911.4 bits (2354), Expect = 6.0e-262
Identity = 439/605 (72.56%), Postives = 514/605 (84.96%), Query Frame = 1

Query: 6   NRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVVVP 65
           NR + QSAAEKAVSVIGFGYDL  D+RLSSCKPGP GS+LI++D  R+++L +P GV+V 
Sbjct: 4   NRLDAQSAAEKAVSVIGFGYDLTKDIRLSSCKPGPFGSRLIELDLARNQELFVPGGVIVQ 63

Query: 66  NVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAG 125
           NVP  I CDKGERTRF SDV+SFNQMSE  NQ++ LSGKIPSG FN MF L+GCWQ DA 
Sbjct: 64  NVPNGIKCDKGERTRFLSDVLSFNQMSEKFNQEMCLSGKIPSGLFNAMFDLRGCWQKDAA 123

Query: 126 STRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKM 185
           S ++LAFDG FITLYN+ELE+SHITLSE+VK EVP++WDP ALAEFIEKYGTH+VVGVKM
Sbjct: 124 SAKNLAFDGWFITLYNVELERSHITLSEEVKYEVPATWDPVALAEFIEKYGTHIVVGVKM 183

Query: 186 GGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYSLPWEL 245
           GGKDV+ ++QL++SN+E  +VQK L++ AD +FS DAN       ++ K KDE+ + W+ 
Sbjct: 184 GGKDVMHIKQLQKSNLEPPEVQKLLREYADKRFSGDANPA----ELSGKPKDEHIMHWDF 243

Query: 246 RNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITS 305
              FA SI+PPVI   K   +T+I IRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITS
Sbjct: 244 HGVFAHSIRPPVITSIKNGHITSICIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITS 303

Query: 306 LMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQASPS 365
           L++G+RG+GFLSHAVNLYLRYKPPIEEL QFLEFQLPRQWAP+Y DLPL  + RKQA+PS
Sbjct: 304 LLSGIRGSGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPIYGDLPLTLKRRKQATPS 363

Query: 366 LKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDDLS 425
           L+FT+MGPKLYVNTT+VDS N PVTG+RL+LEGK+SDHLAIHLQHLS+LP  +QLSDDL 
Sbjct: 364 LRFTIMGPKLYVNTTQVDSGNRPVTGIRLYLEGKRSDHLAIHLQHLSSLPNMVQLSDDLR 423

Query: 426 YRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGMRKVL 485
             P  EP E+GYFEPV WSIFSHVCTAPVEYN + I DDSASIVTKAWFEVK +GM+KVL
Sbjct: 424 QEPTHEPVERGYFEPVNWSIFSHVCTAPVEYNDARI-DDSASIVTKAWFEVKVVGMKKVL 483

Query: 486 FLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELNSAVY 545
           FLRLGFSMVASA +RRS+W+GPST SRKSG+ S  ISTRFSAGL+ PE+  K++LNSAV+
Sbjct: 484 FLRLGFSMVASARLRRSEWDGPSTLSRKSGVFSMLISTRFSAGLNPPEKPVKVDLNSAVF 543

Query: 546 PDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLLTILS 605
           P GPPL SRA KM N +DTKEMVRGPE+ PGYWV+TGAKLCV+GGRISIKVKYSLL I+S
Sbjct: 544 PGGPPLPSRAPKMSNFVDTKEMVRGPEDPPGYWVITGAKLCVDGGRISIKVKYSLLAIMS 603

Query: 606 EDSMI 611
           E+SM+
Sbjct: 604 EESMM 603

BLAST of CSPI01G12360 vs. TrEMBL
Match: V4UER2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10014498mg PE=4 SV=1)

HSP 1 Score: 908.3 bits (2346), Expect = 5.1e-261
Identity = 435/609 (71.43%), Postives = 521/609 (85.55%), Query Frame = 1

Query: 2   SFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAG 61
           S   NR +PQSAAE+AVSVIGFGYDLCND+RLSSCKPGPSG++LI++D + +RDLV P G
Sbjct: 65  SMAYNRLDPQSAAERAVSVIGFGYDLCNDIRLSSCKPGPSGTRLIEMDHSVTRDLVFPCG 124

Query: 62  VVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQ 121
           VVV +V  SI CDKGERTRFRSDV+SFNQMSE  NQ+L+LSGKIPSG FN+MF   G WQ
Sbjct: 125 VVVKDVSISIKCDKGERTRFRSDVLSFNQMSEKFNQELALSGKIPSGLFNSMFKFNGSWQ 184

Query: 122 NDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVV 181
            DA + +SLAFDG FI+LYN+EL +SHI LSEQV++EVP+SWDPAALAEFI+KYGTHVVV
Sbjct: 185 KDASAVKSLAFDGWFISLYNVELARSHIVLSEQVRKEVPTSWDPAALAEFIDKYGTHVVV 244

Query: 182 GVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYSL 241
           GVKMGGKDVI ++QLR SN++  +V K LK LAD +FSEDAN       ++ K+KDE S 
Sbjct: 245 GVKMGGKDVIHVKQLRNSNLQPPEVLKFLKLLADGRFSEDANPA----EISGKLKDETSK 304

Query: 242 PWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFV 301
           PW+L+  +A S+KP ++N SK +++ +I  RRGGVD+ Q+HN+WLST+S SPNVISMSFV
Sbjct: 305 PWDLQGLYANSVKPSIVNQSKNNDMISISSRRGGVDICQTHNRWLSTVSHSPNVISMSFV 364

Query: 302 PITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQ 361
           PITSL+ GV+GNGFL+HAVNLYLRYKPPIEEL QFLEFQLPRQWAPVY DLPL  R RKQ
Sbjct: 365 PITSLLAGVQGNGFLTHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRRKQ 424

Query: 362 ASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLS 421
           ASPSL+F+ +GPKLYVNTT+VDS N PVTG+RL+LEGKKSDHLA+HLQHLST+P++++L+
Sbjct: 425 ASPSLQFSFLGPKLYVNTTQVDSGNRPVTGIRLYLEGKKSDHLAVHLQHLSTVPQSIELT 484

Query: 422 DDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGM 481
           DDLSY P DEP E+GY+EPVKWSIFSHVCTAPV+Y+G+ I DDSASIVTKAW EVK IGM
Sbjct: 485 DDLSYEPTDEPVERGYYEPVKWSIFSHVCTAPVQYDGACI-DDSASIVTKAWLEVKVIGM 544

Query: 482 RKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELN 541
           +KVLFLRLGFS VASA +RRS+W+GPST SRKSG+ S  +ST+FS GL+QPE+  K++LN
Sbjct: 545 KKVLFLRLGFSTVASAKVRRSEWDGPSTMSRKSGVFSMLMSTKFSTGLNQPEKPVKVDLN 604

Query: 542 SAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLL 601
           SAV+P GPPL  +A KM N +DTKEMVRGPE+ PGYWVV+GAKLCVE GRISIK K+SLL
Sbjct: 605 SAVFPGGPPLPPKAPKMTNFVDTKEMVRGPEDPPGYWVVSGAKLCVESGRISIKGKFSLL 664

Query: 602 TILSEDSMI 611
           TI+SEDSM+
Sbjct: 665 TIMSEDSML 668

BLAST of CSPI01G12360 vs. TrEMBL
Match: A0A068TTB7_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00029110001 PE=4 SV=1)

HSP 1 Score: 905.6 bits (2339), Expect = 3.3e-260
Identity = 435/604 (72.02%), Postives = 515/604 (85.26%), Query Frame = 1

Query: 9   NPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVVVPNVP 68
           +PQSAAEKAV  IG GYDL  D+RLS+CK GP+GS+LI++D + +++LV+P GVVVP V 
Sbjct: 20  DPQSAAEKAVGAIGMGYDLTADIRLSACKEGPNGSRLIELDDSSTKELVVPGGVVVPGVS 79

Query: 69  TSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAGSTR 128
           TSI CDKGERTRFRSD +SF+QMSE  NQ+LSLSGKIP G FN MF  KGCWQ DA +T+
Sbjct: 80  TSIKCDKGERTRFRSDFLSFSQMSEQINQELSLSGKIPCGLFNGMFDFKGCWQKDATATK 139

Query: 129 SLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGK 188
            LAFDG FI+LYNIEL KSH+TLSEQVKREVP  WDPAALAEFI+KYGTHV++GVKMGGK
Sbjct: 140 GLAFDGWFISLYNIELSKSHVTLSEQVKREVPPFWDPAALAEFIDKYGTHVILGVKMGGK 199

Query: 189 DVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKI--KDEYSLPWELR 248
           DV+ M+QL+ SN++  DVQK LKKL++++FSE  +G L SNP  + +  KDE S+ W+  
Sbjct: 200 DVVHMKQLQSSNLQPNDVQKLLKKLSEERFSE-TDGSLISNPEGSLVMPKDEKSVLWDPY 259

Query: 249 NAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITSL 308
           + FA  ++P +++H+K  +L +I+IRRGGV+ GQSH +WLST+S SPNVISMSFVPI SL
Sbjct: 260 SLFANPLRPAIVSHTKNADLLSIHIRRGGVNKGQSHAEWLSTVSHSPNVISMSFVPIVSL 319

Query: 309 MNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQASPSL 368
           +NGV+G+GF SHA+NLYLRYKPPIEELQQFLEFQLPRQWAP Y+DLPLAPR RKQASP+L
Sbjct: 320 LNGVKGSGFFSHAMNLYLRYKPPIEELQQFLEFQLPRQWAPAYSDLPLAPRRRKQASPAL 379

Query: 369 KFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDDLSY 428
           +FT MGPKLYVNT KVDS N PVTG+RL+LEGK+SDHLAIHLQHLSTLP T QL+DD SY
Sbjct: 380 QFTFMGPKLYVNTVKVDSGNRPVTGIRLYLEGKRSDHLAIHLQHLSTLPNTYQLTDDHSY 439

Query: 429 RPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGMRKVLF 488
            P+DEP E+GYFEPVKWSIFSHVCTAPVEY G+ I DDSASIVTK+WFEVK +GM+KVLF
Sbjct: 440 EPVDEPVERGYFEPVKWSIFSHVCTAPVEYRGTRI-DDSASIVTKSWFEVKNVGMKKVLF 499

Query: 489 LRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELNSAVYP 548
           LR GFSMVAS+ IRRS+W+GPST SRKSGLIS  +ST FSAGL QP++  K+++NSAVYP
Sbjct: 500 LRFGFSMVASSRIRRSEWDGPSTLSRKSGLISMLMSTTFSAGLGQPDKPVKVDVNSAVYP 559

Query: 549 DGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLLTILSE 608
            GPP  +RA KM N +DTKEM RGPE+SPGYWVVTGAKLCVEGGRI IKVKYSLLTILSE
Sbjct: 560 GGPPSPARAPKMTNFVDTKEMTRGPEDSPGYWVVTGAKLCVEGGRIRIKVKYSLLTILSE 619

Query: 609 DSMI 611
           DS++
Sbjct: 620 DSLL 621

BLAST of CSPI01G12360 vs. TrEMBL
Match: A0A0B0P9N5_GOSAR (MACPF domain-containing NSL1-like protein OS=Gossypium arboreum GN=F383_08312 PE=4 SV=1)

HSP 1 Score: 900.6 bits (2326), Expect = 1.1e-258
Identity = 435/610 (71.31%), Postives = 511/610 (83.77%), Query Frame = 1

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           M+ N ++ +PQSAAEKAVSVIGFGYDL NDLRLS+CKPGPSGS+LI++D   SR+LV P 
Sbjct: 1   MAGNFHQLDPQSAAEKAVSVIGFGYDLYNDLRLSACKPGPSGSRLIELDSVVSRELVFPG 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GV V NVP SI CDKGERTRFRSDV+SFNQMSE  NQ LSLSGKIPSG FN MF  KGCW
Sbjct: 61  GVAVKNVPNSIKCDKGERTRFRSDVLSFNQMSEKFNQDLSLSGKIPSGLFNAMFEFKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           Q DAGST+ LA DG FITLYN+EL+++HITLSE+VK++VP +WDP A+AEFIEKYGTH++
Sbjct: 121 QKDAGSTKCLALDGWFITLYNVELDRAHITLSERVKQDVPETWDPTAIAEFIEKYGTHII 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240
           VGVKMGGKDVI ++Q   SN+   +VQK LK+LAD++FSE+    + S     K K+E  
Sbjct: 181 VGVKMGGKDVIHIKQSNNSNLHPNEVQKTLKQLADERFSENEEAPVFSE----KPKEEPY 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           +PW+L+   AASI+PPV+  SK +++  +YIRRGGVD GQSH+QWLSTISQSPNVISM F
Sbjct: 241 MPWDLQGMPAASIRPPVVTRSKNNDIVNVYIRRGGVDYGQSHSQWLSTISQSPNVISMKF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSL+ G RGNGFLSHAVNLYLRYKPPIEEL++FLEFQ+PRQWAPVY DLPL+ + RK
Sbjct: 301 VPITSLLGGSRGNGFLSHAVNLYLRYKPPIEELREFLEFQVPRQWAPVYGDLPLSLKRRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSL+FT MGPKLYVNT  VD+ N PVTG+RL+LEGKK+DHLAIHLQHLSTLP  LQL
Sbjct: 361 QASPSLQFTFMGPKLYVNTVPVDTGNRPVTGIRLYLEGKKNDHLAIHLQHLSTLPTILQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480
           SDD +Y P DEP  +GYFEPVKWSIFSHV TAP++YNGS   DD+A+IVTKAWFEVK +G
Sbjct: 421 SDDHNYEPNDEPLGRGYFEPVKWSIFSHVYTAPIQYNGSHF-DDTAAIVTKAWFEVKSVG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMV+   IRRS+W+GPST SRKSG+ S  ISTRFS+GL QPE+  K++L
Sbjct: 481 MRKVLFLRLGFSMVSFTKIRRSEWDGPSTLSRKSGVFSMLISTRFSSGLTQPEKPTKVDL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAV+P GPPL  +A K+ + +DT E VRGPE+ PGYWVVTGAKLCVEGG+ISIK KYSL
Sbjct: 541 NSAVFPGGPPLPPKAPKLTSFVDTNETVRGPEDLPGYWVVTGAKLCVEGGKISIKAKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSM+
Sbjct: 601 LTILSEDSMM 605

BLAST of CSPI01G12360 vs. TAIR10
Match: AT1G28380.1 (AT1G28380.1 MAC/Perforin domain-containing protein)

HSP 1 Score: 788.1 bits (2034), Expect = 3.9e-228
Identity = 390/612 (63.73%), Postives = 474/612 (77.45%), Query Frame = 1

Query: 4   NINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVV 63
           N  R +  SAAEKAVSVIG GYDLC+D+R S+CK  P GS+L++ID TR+RDL+ P G+V
Sbjct: 5   NFTRLDAHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIV 64

Query: 64  VPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQND 123
           V NV +SI CDKGERTR RSD++SFNQMSE  NQ + LSGKIPSG FN MF    CW  D
Sbjct: 65  VNNVSSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKD 124

Query: 124 AGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGV 183
           A S ++LA+DG FI+LY++E+ +  +TL ++VKREVPSSWD AALA FIEKYGTHVVVGV
Sbjct: 125 ASSVKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGV 184

Query: 184 KMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSED-ANGGLTSNPVAAKIKDEYSLP 243
            MGGKDVI ++Q+R+SN E  ++QK LK   D++F  D       ++  + K K+E  L 
Sbjct: 185 TMGGKDVIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQ 244

Query: 244 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVP 303
           W L+  F  S+   V+ H+K +E+  + IRRGGVD+GQSH +WLST+SQ+PNVISM FVP
Sbjct: 245 WGLQ-PFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVP 304

Query: 304 ITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQA 363
           ITSL++G+ G GFLSHAVNLYLRYKPPIEEL QFLEFQLPRQWAPVY DLPL  R  KQ+
Sbjct: 305 ITSLLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQS 364

Query: 364 SPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSD 423
           SPSL+F+LMGPKLYVNT+KVDS   PVTG+R FLEGKK +HLAIHLQHLS  P +L LS 
Sbjct: 365 SPSLQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSH 424

Query: 424 DLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGMR 483
           D +Y PI+EP EKGY+ PVKW IFSHVCT PV+YNG+   DD+ASIVTKAW EVKG+GMR
Sbjct: 425 DDTYEPIEEPVEKGYYVPVKWGIFSHVCTYPVQYNGAR-SDDTASIVTKAWLEVKGMGMR 484

Query: 484 KVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLH----QPEEQPKI 543
           KVLFLRLGFS+ ASA  R+S W+  ST SRKSG+ S  ISTR S GL       + Q KI
Sbjct: 485 KVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKI 544

Query: 544 ELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKY 603
           ++NSAVYP GP    +  K+L+++DTKE++RGPE  PGYWVVTGAKLCVE G+ISIK KY
Sbjct: 545 DINSAVYPRGPSPPVKP-KLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKY 604

Query: 604 SLLTILSEDSMI 611
           SLLT++SEDS++
Sbjct: 605 SLLTVISEDSLV 612

BLAST of CSPI01G12360 vs. TAIR10
Match: AT4G24290.2 (AT4G24290.2 MAC/Perforin domain-containing protein)

HSP 1 Score: 626.3 bits (1614), Expect = 2.0e-179
Identity = 318/610 (52.13%), Postives = 419/610 (68.69%), Query Frame = 1

Query: 7   RFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDI-DFTRSRDLVLPAGVVVP 66
           R     AAE A+  IG GYDL  DLRL  CK G   S+L+DI +   + ++VLP G+ +P
Sbjct: 4   RLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIP 63

Query: 67  NVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAG 126
           NV  SI CDKGER RFRSD++ F QM+E  NQ+LSL+GKIPSG FN MF    CWQ DA 
Sbjct: 64  NVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAA 123

Query: 127 STRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKM 186
            T++LAFDG FI+LY++ L+KS + L E VK+ VPS+WDPAALA FI+ YGTH++V VKM
Sbjct: 124 YTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKM 183

Query: 187 GGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDA---NGGLTSNPVAAKIKDEYSLP 246
           GGKDVI+ +Q   S ++  D+QK+LK++AD +F E +   N G      ++K++ +    
Sbjct: 184 GGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQ-- 243

Query: 247 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQ-SHNQWLSTISQSPNVISMSFV 306
             LR A  +S+     +++  ++   +  RRGG D     HN+WL T+   P+VISMSF+
Sbjct: 244 -RLRFADTSSLG----SYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFI 303

Query: 307 PITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQ 366
           PITSL+NGV G+GFLSHA+NLYLRYKPPIEEL QFLEFQLPRQWAPV+++LPL P+ ++Q
Sbjct: 304 PITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQ 363

Query: 367 ASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLS 426
           +  SL+F+  GPKLYVNTT VD    P+TG+RL+LEG++S+ LAIHLQHLS+LPK  QL 
Sbjct: 364 SCASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLE 423

Query: 427 DDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGM 486
           DDL+     E  ++ Y+E V W  +SHVCT PVE       DD  S+VT A   V+  G 
Sbjct: 424 DDLNRSIRQESHDRRYYEKVNWKNYSHVCTEPVE------SDDDLSVVTGAQLHVESHGF 483

Query: 487 RKVLFLRLGFSMVASAG-IRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 546
           + VLFLRL FS V  A  ++ S+W+     + KSGLIST IS  F+A    P     + +
Sbjct: 484 KNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNI 543

Query: 547 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 606
           NSA+YP GPP+ ++A K+L  +DT EM RGP+ SPGYWVV+GA+L VE G+IS+KVKYSL
Sbjct: 544 NSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 600

Query: 607 LTILSEDSMI 611
            T +  D +I
Sbjct: 604 FTPILGDEVI 600

BLAST of CSPI01G12360 vs. TAIR10
Match: AT1G14780.1 (AT1G14780.1 MAC/Perforin domain-containing protein)

HSP 1 Score: 464.2 bits (1193), Expect = 1.3e-130
Identity = 256/612 (41.83%), Postives = 375/612 (61.27%), Query Frame = 1

Query: 15  EKAVSVIGFGYDLCNDLRLSSCKPGPSGS---KLIDIDFTRSRDLVLPAGVVVPNVPTSI 74
           E AV  +G G+DL  D RL  CK G   +   +L+ +D T++R+L +P   V  NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 75  NCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLK-GCWQNDAGSTRSL 134
           NCDKGERTRFRSD++ FN+MSE  NQ+ S++GKIPSG FN  FG + G W  DA + +SL
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 135 AFDGCFITLYNIELEK-SHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKD 194
             D   +TL+N+ +   + + L+++V+  VPSSWDP  LA FIE+YGTHV+ GV +GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 195 VIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYSLPW--ELRN 254
           V+ +RQ + S+++   ++  L  L D  F+      L++  +       +S P   E  N
Sbjct: 190 VVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCL--LSTRRLNKAYHHSHSQPKFPEAFN 249

Query: 255 AFAASIKPPVINHSKGDE--LTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITS 314
            F         N S   +  +T I  +RGG    +SH++WL T+   P+ I+ +F+PITS
Sbjct: 250 VFDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITS 309

Query: 315 LMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPL-APRHRKQASP 374
           L+  V G+G LSHA++LYLRYKPP+ +LQ FL+F  PR WAPV+ DLP  A  +   A P
Sbjct: 310 LLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYP 369

Query: 375 SLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTL--QLSD 434
           +L    MGPKLYVNTT V S   PVTG+R FLEGKK + LAIHLQHL     T+  +++D
Sbjct: 370 ALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITD 429

Query: 435 DLSYRPIDE-PEEKGYFEPVKWSIFSHVCTAPVEYNGSLI--------DDDSASIVTKAW 494
           +  +R  D+  +   YFEP+    FSHVCT PV+Y+ + I         +D A IVT A 
Sbjct: 430 EHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTGAQ 489

Query: 495 FEVKGIGMRKVLFLRLGFSMVASAGIRRSDW-EGPSTTSRKSGLISTFISTRFSAGLHQ- 554
            EVK  G + VL LRL ++ V+   + ++ W  GP  TS+KSG+ S+      S  +H  
Sbjct: 490 LEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVHHN 549

Query: 555 --PEEQPKIELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEG 602
              +++ ++ L+S V+P GPP+ +   K++  +D  ++ RGP++SPG+W+VTG +L ++ 
Sbjct: 550 MIQKDKNEVVLDSGVFPGGPPVPANN-KIVKFVDLSQLCRGPQHSPGHWLVTGVRLYLDK 609

BLAST of CSPI01G12360 vs. TAIR10
Match: AT1G29690.1 (AT1G29690.1 MAC/Perforin domain-containing protein)

HSP 1 Score: 425.2 bits (1092), Expect = 6.6e-119
Identity = 239/600 (39.83%), Postives = 349/600 (58.17%), Query Frame = 1

Query: 17  AVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVVVPNVPTSINCDKG 76
           A+  +G G+D+ +D+RL  CK  P GS+L+ I+  ++RDL L  G ++PNVP  I+C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKGAP-GSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 77  ERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAGSTRSLAFDGCF 136
                R  V SF++M+E  N +  + G IP G FN MF   G WQ DA ST+SLA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 137 ITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKDVIFMRQL 196
           I LY+++L K  + L  +++R VPSSWDPA+LA FIE YGTH+V  V +GG+DV+++RQ 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 197 RESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYSLPWELRNAFAASIKPP 256
           + S +  ++++  +  +   +F E  +  +T  P+  K KD                   
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHEAESQSITG-PLKYKDKD------------------- 263

Query: 257 VINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITSLMNGVRGNGFL 316
                    +T I+ RRGG D+ QSH +W  T+  +P++I+M+F PI SL+ GV G   L
Sbjct: 264 ---------ITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHL 323

Query: 317 SHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQASPSLKFTLMGPKLY 376
           + A+ LYL YKPPIE+LQ FL++Q+ R WAP  ++L    + ++    SL+F+LMGPKL+
Sbjct: 324 TRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLF 383

Query: 377 VNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDDLSYRPIDEPEEKG 436
           ++  +V     PVTG+RL LEG K + L+IHLQHL +LPK LQ   D S+ PI  P+ +G
Sbjct: 384 ISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWD-SHVPIGAPKWQG 443

Query: 437 -------YFEPVKWSIFSHVCTAPVEYNGSLIDDDSA-SIVTKAWFEVKGIGMRKVLFLR 496
                  +FEP+KW  FSHV T+P+E+  + I D S   IVT A   V   G + VL L+
Sbjct: 444 PEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLK 503

Query: 497 LGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELNSAVYPDG 556
           L FS V    IRRS W+                        H P     +  +  + P G
Sbjct: 504 LLFSKVPGCTIRRSVWD------------------------HTP-----VASSGRLEPGG 559

Query: 557 PPLTS-------RAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLL 602
           P  +S       ++ K+  ++D+ EM++GP++ PG+W+VTGAKL VE G+I ++VKYSLL
Sbjct: 564 PSTSSSTEEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559

BLAST of CSPI01G12360 vs. NCBI nr
Match: gi|449457843|ref|XP_004146657.1| (PREDICTED: MACPF domain-containing protein NSL1 [Cucumis sativus])

HSP 1 Score: 1235.7 bits (3196), Expect = 0.0e+00
Identity = 610/610 (100.00%), Postives = 610/610 (100.00%), Query Frame = 1

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480
           SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of CSPI01G12360 vs. NCBI nr
Match: gi|1009109532|ref|XP_015890671.1| (PREDICTED: MACPF domain-containing protein NSL1 [Ziziphus jujuba])

HSP 1 Score: 934.9 bits (2415), Expect = 7.3e-269
Identity = 447/605 (73.88%), Postives = 523/605 (86.45%), Query Frame = 1

Query: 8   FNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVVVPNV 67
           FNPQSAA+KAVS+IGFGYDLCND+RL SCKPGPSGS+LI+ D T++RDLV+P GVVVP+V
Sbjct: 6   FNPQSAADKAVSIIGFGYDLCNDIRLPSCKPGPSGSRLIEFDQTQTRDLVVPGGVVVPDV 65

Query: 68  PTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAGST 127
            TSI CDKGERTRFRSDV SFNQMSE  N++LSLSGKIP G FN MF  KGCWQ DA   
Sbjct: 66  STSIKCDKGERTRFRSDVFSFNQMSEQFNKELSLSGKIPCGLFNAMFDFKGCWQKDATPV 125

Query: 128 RSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGG 187
           +SL+FDG FITLYNIEL +SHITLS+ VK+EVPSSWDP ALAEFIEKYGTH+VVGV+MGG
Sbjct: 126 KSLSFDGWFITLYNIELGRSHITLSKHVKQEVPSSWDPFALAEFIEKYGTHIVVGVQMGG 185

Query: 188 KDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNP--VAAKIKDEYSLPWEL 247
           KDV+ ++QL+ SN+   +VQK+LK+LAD++FSED NG   SNP  ++ K+KDE+ L  +L
Sbjct: 186 KDVVHIKQLQNSNLHPIEVQKQLKQLADERFSEDVNGNSASNPAELSGKMKDEHFLGLDL 245

Query: 248 RNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITS 307
           R AFA S +PP+I HSK D++ +I +RRGG+D+GQSHNQWLST+SQSPNVISMSFVPITS
Sbjct: 246 RKAFATSTRPPIITHSKNDDIVSISVRRGGIDIGQSHNQWLSTVSQSPNVISMSFVPITS 305

Query: 308 LMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQASPS 367
           L+ GV+GNGFLSHAVNLYLRYKPPIEEL QFLEFQLPRQWAP Y DLPL  R RKQ SPS
Sbjct: 306 LLGGVQGNGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPAYGDLPLTLRRRKQPSPS 365

Query: 368 LKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDDLS 427
           L+FT +GPKLYVNT KVDS N PVTG+RL+LEGKK+DHLAIHLQHLSTLP  LQL DD S
Sbjct: 366 LQFTFLGPKLYVNTMKVDSGNRPVTGIRLYLEGKKNDHLAIHLQHLSTLPNNLQLLDDHS 425

Query: 428 YRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGMRKVL 487
           +  I+EP E+ YFEPVKWSIFSHV TAPV+YNG+ I D+SASIVTKAWFEVK IGM+KVL
Sbjct: 426 HESINEPVERAYFEPVKWSIFSHVYTAPVQYNGACI-DESASIVTKAWFEVKTIGMKKVL 485

Query: 488 FLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELNSAVY 547
           FLRLGFSMVASA IRRS+WEGP+  SRKSG +S  ISTRFS+GL+ P++  K++LNSA+Y
Sbjct: 486 FLRLGFSMVASARIRRSEWEGPTNLSRKSGFLSVLISTRFSSGLNPPQKPAKVDLNSAIY 545

Query: 548 PDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLLTILS 607
           P+GPP+ ++A K+ N +DTKEMVRGPE+ PGYW+VTGAKLCVEGGRISIKVKYSLLTI+S
Sbjct: 546 PEGPPMPTKAPKLANFVDTKEMVRGPEDPPGYWMVTGAKLCVEGGRISIKVKYSLLTIMS 605

Query: 608 EDSMI 611
           EDSM+
Sbjct: 606 EDSMM 609

BLAST of CSPI01G12360 vs. NCBI nr
Match: gi|743846626|ref|XP_011027785.1| (PREDICTED: MACPF domain-containing protein NSL1 [Populus euphratica])

HSP 1 Score: 911.8 bits (2355), Expect = 6.6e-262
Identity = 440/605 (72.73%), Postives = 513/605 (84.79%), Query Frame = 1

Query: 6   NRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVVVP 65
           NR + QSAAEKAVSV+GFGYDL  D+RLSSCKPG  GS+LI++D TR+++L +P GVVV 
Sbjct: 4   NRLDAQSAAEKAVSVVGFGYDLTKDIRLSSCKPGSFGSRLIELDLTRNQELFVPGGVVVQ 63

Query: 66  NVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAG 125
           NVP  I CDKGERTRF SDV+SFNQMSE  NQ++ L+GKIPSG FN MF L+GCWQ DA 
Sbjct: 64  NVPNGIKCDKGERTRFLSDVLSFNQMSEKFNQEMCLAGKIPSGLFNAMFDLRGCWQKDAA 123

Query: 126 STRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKM 185
           S ++LAFDG FITLYN+ELE+SHITLSE+VK EVP++WDP ALAEFI+KYGTHVVVGVKM
Sbjct: 124 SAKNLAFDGWFITLYNVELERSHITLSEEVKHEVPATWDPVALAEFIDKYGTHVVVGVKM 183

Query: 186 GGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYSLPWEL 245
           GGKDV+ ++QL++SN+E  +VQK LK+ AD +FS D N       ++ K KDE+ +PW+ 
Sbjct: 184 GGKDVMHIKQLQKSNLEPPEVQKLLKEYADKRFSGDVNPA----ELSGKPKDEHIIPWDF 243

Query: 246 RNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITS 305
              FA S +PPVI   K   +T+I IRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITS
Sbjct: 244 HGVFAHSFRPPVIASIKNGHITSICIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITS 303

Query: 306 LMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQASPS 365
           L++G+RGNGFLSHAVNLYLRYKPPIEEL QFLEFQLPRQWAP+Y DLPL  + RKQA+PS
Sbjct: 304 LLSGIRGNGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPIYGDLPLTLKRRKQATPS 363

Query: 366 LKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDDLS 425
           L+FT+MGPKLYVNTT+VDS N PVTG+RL+LEGK+SDHLAIHLQHLS+LP  +QLSDDL 
Sbjct: 364 LRFTIMGPKLYVNTTQVDSGNRPVTGIRLYLEGKRSDHLAIHLQHLSSLPNMVQLSDDLR 423

Query: 426 YRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGMRKVL 485
             P  EP E+GYFEPV WSIFSHVCTAPVEYN + I DDSASIVTKAWFEVK +GM+KVL
Sbjct: 424 QEPTHEPVERGYFEPVNWSIFSHVCTAPVEYNEARI-DDSASIVTKAWFEVKVVGMKKVL 483

Query: 486 FLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELNSAVY 545
           FLRLGFSMVASA +RRS+W+GPST SRKSG+ S  ISTRFSAGL+ PE+  K++LNSAV+
Sbjct: 484 FLRLGFSMVASARLRRSEWDGPSTLSRKSGVFSMLISTRFSAGLNPPEKPVKVDLNSAVF 543

Query: 546 PDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLLTILS 605
           P GPPL SRA KM N +DTKEMVRGPE+ PGYWVVTGAKLCV+GGRISIKVKYSLL I+S
Sbjct: 544 PGGPPLPSRAPKMSNFVDTKEMVRGPEDPPGYWVVTGAKLCVDGGRISIKVKYSLLAIMS 603

Query: 606 EDSMI 611
           E+SM+
Sbjct: 604 EESMM 603

BLAST of CSPI01G12360 vs. NCBI nr
Match: gi|566164952|ref|XP_002305779.2| (hypothetical protein POPTR_0004s04730g [Populus trichocarpa])

HSP 1 Score: 911.4 bits (2354), Expect = 8.6e-262
Identity = 439/605 (72.56%), Postives = 514/605 (84.96%), Query Frame = 1

Query: 6   NRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVVVP 65
           NR + QSAAEKAVSVIGFGYDL  D+RLSSCKPGP GS+LI++D  R+++L +P GV+V 
Sbjct: 4   NRLDAQSAAEKAVSVIGFGYDLTKDIRLSSCKPGPFGSRLIELDLARNQELFVPGGVIVQ 63

Query: 66  NVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAG 125
           NVP  I CDKGERTRF SDV+SFNQMSE  NQ++ LSGKIPSG FN MF L+GCWQ DA 
Sbjct: 64  NVPNGIKCDKGERTRFLSDVLSFNQMSEKFNQEMCLSGKIPSGLFNAMFDLRGCWQKDAA 123

Query: 126 STRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKM 185
           S ++LAFDG FITLYN+ELE+SHITLSE+VK EVP++WDP ALAEFIEKYGTH+VVGVKM
Sbjct: 124 SAKNLAFDGWFITLYNVELERSHITLSEEVKYEVPATWDPVALAEFIEKYGTHIVVGVKM 183

Query: 186 GGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYSLPWEL 245
           GGKDV+ ++QL++SN+E  +VQK L++ AD +FS DAN       ++ K KDE+ + W+ 
Sbjct: 184 GGKDVMHIKQLQKSNLEPPEVQKLLREYADKRFSGDANPA----ELSGKPKDEHIMHWDF 243

Query: 246 RNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITS 305
              FA SI+PPVI   K   +T+I IRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITS
Sbjct: 244 HGVFAHSIRPPVITSIKNGHITSICIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITS 303

Query: 306 LMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQASPS 365
           L++G+RG+GFLSHAVNLYLRYKPPIEEL QFLEFQLPRQWAP+Y DLPL  + RKQA+PS
Sbjct: 304 LLSGIRGSGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPIYGDLPLTLKRRKQATPS 363

Query: 366 LKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDDLS 425
           L+FT+MGPKLYVNTT+VDS N PVTG+RL+LEGK+SDHLAIHLQHLS+LP  +QLSDDL 
Sbjct: 364 LRFTIMGPKLYVNTTQVDSGNRPVTGIRLYLEGKRSDHLAIHLQHLSSLPNMVQLSDDLR 423

Query: 426 YRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGMRKVL 485
             P  EP E+GYFEPV WSIFSHVCTAPVEYN + I DDSASIVTKAWFEVK +GM+KVL
Sbjct: 424 QEPTHEPVERGYFEPVNWSIFSHVCTAPVEYNDARI-DDSASIVTKAWFEVKVVGMKKVL 483

Query: 486 FLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELNSAVY 545
           FLRLGFSMVASA +RRS+W+GPST SRKSG+ S  ISTRFSAGL+ PE+  K++LNSAV+
Sbjct: 484 FLRLGFSMVASARLRRSEWDGPSTLSRKSGVFSMLISTRFSAGLNPPEKPVKVDLNSAVF 543

Query: 546 PDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLLTILS 605
           P GPPL SRA KM N +DTKEMVRGPE+ PGYWV+TGAKLCV+GGRISIKVKYSLL I+S
Sbjct: 544 PGGPPLPSRAPKMSNFVDTKEMVRGPEDPPGYWVITGAKLCVDGGRISIKVKYSLLAIMS 603

Query: 606 EDSMI 611
           E+SM+
Sbjct: 604 EESMM 603

BLAST of CSPI01G12360 vs. NCBI nr
Match: gi|698587940|ref|XP_009779321.1| (PREDICTED: MACPF domain-containing protein NSL1 [Nicotiana sylvestris])

HSP 1 Score: 909.1 bits (2348), Expect = 4.3e-261
Identity = 443/613 (72.27%), Postives = 525/613 (85.64%), Query Frame = 1

Query: 1   MSFNI-NRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLP 60
           M+FN  +R +PQ+AAEKAVSVIGFGYDL +D+RL++CKPGPSGS LI++D   ++DLV+P
Sbjct: 1   MAFNPGSRMDPQAAAEKAVSVIGFGYDLTSDIRLTACKPGPSGSGLIEVDQKSTKDLVVP 60

Query: 61  AGVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGC 120
            GVVV  V TSI CDKGERTRFRSD +SF+QMSE  NQ+LSLSGKIPSG FN MFG KGC
Sbjct: 61  GGVVVSKVSTSIKCDKGERTRFRSDALSFSQMSEQLNQELSLSGKIPSGLFNAMFGHKGC 120

Query: 121 WQNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHV 180
           WQ DA ST+ LAFDG FITLYNIEL +SH+TLSEQVK++VPSSWDP+ALAEFIEKYGTH+
Sbjct: 121 WQKDAASTKLLAFDGWFITLYNIELVRSHLTLSEQVKQDVPSSWDPSALAEFIEKYGTHI 180

Query: 181 VVGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAA--KIKD 240
           VVGVKMGGKDVI ++QL+ S ++  +VQK LK+LAD+KFSED NG    N V +  K KD
Sbjct: 181 VVGVKMGGKDVIHIKQLQNSTLQPMEVQKLLKQLADEKFSEDVNGCQMRNSVKSSEKPKD 240

Query: 241 EYSLPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVIS 300
           E S+  +    FA SI+P +++HSK D+L +I+IRRGG+D GQ H+QWL T+SQSPNVIS
Sbjct: 241 EKSIFSDPHLPFANSIRPSIMSHSKADDLLSIHIRRGGLDFGQRHSQWLPTVSQSPNVIS 300

Query: 301 MSFVPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPR 360
           MSFVPI SL++GVRG+GFLSHA+NLYLRYKPPIEEL+QFLEFQLPRQWAP Y+DLPL  R
Sbjct: 301 MSFVPIASLLSGVRGSGFLSHAINLYLRYKPPIEELEQFLEFQLPRQWAPAYSDLPLGHR 360

Query: 361 HRKQASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKT 420
           HRKQ+SPSL+FTL+GP+LYVNT KVDS N PVTG+RL+LEGK+SDHLAIHLQHLS LP++
Sbjct: 361 HRKQSSPSLQFTLLGPRLYVNTIKVDSGNRPVTGIRLYLEGKRSDHLAIHLQHLSALPQS 420

Query: 421 LQLSDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVK 480
           +QL+DDLSY P+DEP E+GYFEPVKWSIFSH+CTAPVEY G+ I DDSASIVTKAWFEVK
Sbjct: 421 IQLTDDLSYEPVDEPVERGYFEPVKWSIFSHICTAPVEYRGTRI-DDSASIVTKAWFEVK 480

Query: 481 GIGMRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPK 540
            IGM+KVLFLRLGFSMVASA IRRS+WEGP++T RKSGL+S  IST FS  L+QP++  K
Sbjct: 481 VIGMKKVLFLRLGFSMVASAKIRRSEWEGPASTCRKSGLMSMLISTPFSTKLNQPQKPAK 540

Query: 541 IELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVK 600
           ++LNSAVYP GPP  +RA KM + +DT EMVRGPE SPGYWVVTGAKLCVE  RI +KVK
Sbjct: 541 VDLNSAVYPGGPPSPARAPKMSHFVDTTEMVRGPEESPGYWVVTGAKLCVEDSRIRMKVK 600

Query: 601 YSLLTILSEDSMI 611
           YSLLTIL+EDS++
Sbjct: 601 YSLLTILTEDSLL 612

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
NSL1_ARATH6.9e-22763.73MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana GN=NSL1 PE=2 SV=1[more]
MACP2_ARATH3.5e-17852.13MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana GN=At4g24290 P... [more]
MACP1_ARATH2.3e-12941.83MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana GN=At1g14780 P... [more]
CAD1_ARATH1.2e-11739.83MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana GN=CAD1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LV66_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G075560 PE=4 SV=1[more]
B9H2R6_POPTR6.0e-26272.56Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0004s04730g PE=4 SV=2[more]
V4UER2_9ROSI5.1e-26171.43Uncharacterized protein OS=Citrus clementina GN=CICLE_v10014498mg PE=4 SV=1[more]
A0A068TTB7_COFCA3.3e-26072.02Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00029110001 PE=4 SV=1[more]
A0A0B0P9N5_GOSAR1.1e-25871.31MACPF domain-containing NSL1-like protein OS=Gossypium arboreum GN=F383_08312 PE... [more]
Match NameE-valueIdentityDescription
AT1G28380.13.9e-22863.73 MAC/Perforin domain-containing protein[more]
AT4G24290.22.0e-17952.13 MAC/Perforin domain-containing protein[more]
AT1G14780.11.3e-13041.83 MAC/Perforin domain-containing protein[more]
AT1G29690.16.6e-11939.83 MAC/Perforin domain-containing protein[more]
Match NameE-valueIdentityDescription
gi|449457843|ref|XP_004146657.1|0.0e+00100.00PREDICTED: MACPF domain-containing protein NSL1 [Cucumis sativus][more]
gi|1009109532|ref|XP_015890671.1|7.3e-26973.88PREDICTED: MACPF domain-containing protein NSL1 [Ziziphus jujuba][more]
gi|743846626|ref|XP_011027785.1|6.6e-26272.73PREDICTED: MACPF domain-containing protein NSL1 [Populus euphratica][more]
gi|566164952|ref|XP_002305779.2|8.6e-26272.56hypothetical protein POPTR_0004s04730g [Populus trichocarpa][more]
gi|698587940|ref|XP_009779321.1|4.3e-26172.27PREDICTED: MACPF domain-containing protein NSL1 [Nicotiana sylvestris][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR020864MACPF
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043090 amino acid import
biological_process GO:0015696 ammonium transport
biological_process GO:0008150 biological_process
biological_process GO:0009862 systemic acquired resistance, salicylic acid mediated signaling pathway
biological_process GO:0009697 salicylic acid biosynthetic process
biological_process GO:0009651 response to salt stress
biological_process GO:0034976 response to endoplasmic reticulum stress
biological_process GO:0010200 response to chitin
biological_process GO:0002679 respiratory burst involved in defense response
biological_process GO:0010337 regulation of salicylic acid metabolic process
biological_process GO:0010363 regulation of plant-type hypersensitive response
biological_process GO:0043900 regulation of multi-organism process
biological_process GO:0043269 regulation of ion transport
biological_process GO:0010310 regulation of hydrogen peroxide metabolic process
biological_process GO:0006612 protein targeting to membrane
biological_process GO:0006862 nucleotide transport
biological_process GO:0043069 negative regulation of programmed cell death
biological_process GO:0031348 negative regulation of defense response
biological_process GO:0000165 MAPK cascade
biological_process GO:0009867 jasmonic acid mediated signaling pathway
biological_process GO:0006888 ER to Golgi vesicle-mediated transport
biological_process GO:0006984 ER-nucleus signaling pathway
biological_process GO:0009595 detection of biotic stimulus
biological_process GO:0050832 defense response to fungus
biological_process GO:0042742 defense response to bacterium
biological_process GO:0052542 defense response by callose deposition
biological_process GO:0015802 basic amino acid transport
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G12360.1CSPI01G12360.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020864Membrane attack complex component/perforin (MACPF) domainPFAMPF01823MACPFcoord: 110..323
score: 4.3
IPR020864Membrane attack complex component/perforin (MACPF) domainSMARTSM00457MACPF_8coord: 127..325
score: 2.6
IPR020864Membrane attack complex component/perforin (MACPF) domainPROFILEPS51412MACPF_2coord: 2..337
score: 23
NoneNo IPR availablePANTHERPTHR33199FAMILY NOT NAMEDcoord: 1..610
score:
NoneNo IPR availablePANTHERPTHR33199:SF8MACPF DOMAIN-CONTAINING PROTEIN NSL1coord: 1..610
score:

The following gene(s) are paralogous to this gene:

None