Csa6G519670 (gene) Cucumber (Chinese Long) v2

Overview
NameCsa6G519670
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionZinc finger family protein; contains IPR011016 (Zinc finger, RING-CH-type), IPR013083 (Zinc finger, RING/FYVE/PHD-type)
LocationChr6 : 27663525 .. 27666372 (+)
Genome Viewer
Gene Expression
   



Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGCTTCCCATAAAGATGAATTGTATTCTATTCAATTTCCCTCTCTTTACTATTTCTTAAATCGTTTACTTAATACCTAATTTTACACTCTCCCCTCAGATTAGTGTTTATCCTTTACTTCTTCTTCCTTTTATTTTTTTTTATGTCACATCCAGATACTACTGCCAGAGAGAGAGACAGGAGGAAATAGAGGTATTTCACCTGGAGGAGGCCATTTGGAGCCGACCCAGATGACCAGATCTCTCTGTTTTGTTGTTTTCATTTTCAATTTATGAAGTAAACGCTTTCCCTGGAAGAATTTTTCGTATTTAAAAGTAAACTCTCTACTCATTTTCTTCTGTGGAGTTTCTCCTCAGGAACAAGGCATTTATATCTTTCATTGGTGAAACAAAGCTGTAACCAAAATGATGTTTCTCATCATGTACACTAAAGCCTTTGGATTATAAGATGGGGCGATTCTTTTGAGTTTGTTTTTCTCCCTTTTCGTGGGGGATTACCCACTTTCATTTTCGACTGAAATTTTCATTATGTATCAATTAGTTGAGTGATGCATTCTGATACTGTGGTGAGTTACTAGTTTTGTGAACGAAGTATTTCGGAACAGCTTTTGCGGGAATCTGGGTTGACAAGAAAAATATTTTTTGGTAAATGGATCGCGAAGAAAGGAATCGCCAAGATGGTGGTGATGTGTCGAGTCGAGTAGAAACAGCGGTGCCTAAACCCACAAATGATTTGGTTAATGGCGATAGGATCGAGGCTACCATTGTGATTATCCCAGAAAGCCTCGCCTCTGCTGGCGGCGACAAGGAAGCTCACGGCTTCGGTGGAGAAGCTATACCAAATGTTAATATAGAGACAAGGGAGGGTTCGAATCTGAACCCTTCAGAAACTGTACTGGTTATTGATTCGGATGGGGCTGCATCTAGAGGTGAAGACGGAGAATCGCTTGTGAAGAACAATGGAATGGGGTTGGGGAAGGATTTGGAAAAGTTGAATGTAAAATCAAAGATGTCAGAAGCGAAGGACAATCCGATGTTGATAAATGTAAAGCCAAAAGGGGGTAAAGGGTTTATTGATGACTGGAATGGAGAAAGGGTCTGTAGGATCTGCCATTTGGCGTCCAATCAAACCTCAGAGGCTAAAACTGGGACCAGTACTTCAGAATTGATTCAACTTGGTTGTGACTGCAAAGATGAGCTGGGAATTGCGCATGGTCACTGTGCTGAGGCATGGTTCAAGCTCAAAGGAAACAGGTTCTTTCTTCTCTCTCTCTCTTCTAGTTTGATATGAAGCTATGTACCGTTTATTTAAGATTTGGCATTGACATTTGATGAAGTGTTGTGGTTTTGGTTATCTATTTTTGTTCTATTACTTTGGCTGCTAATCCGGCCATTTAACTAATACTTTTGACTTTTATCTTTCCTCATTTGTTTTATAATCTAGTCTTCTTTTGGCTCTTTTGCGTCCAAAAGGATTACCCGATTTAGTTTACTGACCTGTAGAAATAGTAACCCCTTTCATTTCTGTAAGCACACAAATTAAAAGGAGCCAGTTTATGAATATGGAGGAAGAAAGTTTAAGGCTGTATCCATCCTTTTGTTGGTTTCTGTCATATTGTAAAAGTCTCCTGTAAAGTTAGTCTACCCTTGATTTTGTTTTGTGCTATCAATTTAAATCTGTCGCTGCTATACCAACTTGGTTCTTCCTTCAGGTATAGTATTGGAAAATTGTAATAAGGGTTCATCGAATTAAGTATCTCTTCCCCTAAAGCGTTTTTCAAGGTGGCATGAGCAGTTATACAATCAATGAAATGGAGTAATGACATAAAGTTACAATCACGTGTCTAAGGGGTTCAAATATGGTTATAGAATATTATGAAAGGGTTACAAAATGTTTATTTTTATCACCTCTTTTGGTTCAAACTTAGAACTTCCCATTTCCAGGTGTCCCTTCAACCTCTTCAAGAGGACTTTAATACTAACTCTAGAGTTGGGAGTGATATTTGATGGCTGATTGTGTTTGAACAATTCAATGATAGATTTAAGGATAATTTTTGTTTAACAGCTTTTGGCGTTTCACATAGATACTGCCAAATGGATTGTAAAGCAAGTTGAAACGAAGTAATTTCCTTGAAGTTGAAATATGCTGCACAAGAGATTGAGTATTGAATGGTATAACATTGATAAAGTCAACTACTTGTTTCTGTGCATTTGCATGTGAAAATAAAAGGAAAAAAAATCCAAAACGATGATTTGCCAATTGGCAACTCCTGTTTTTAAATTACATTGTCTTTCATATTGTCAAATGAGCCGATGATTATCTAATCATAATCATTTTGGTTAATACAGAATGTGCGAAATTTGTGGTGAGACTGCTAAAAACGTTGAAGGAGTTGGGGACAACAGATTTATGGAAGAGTGGAACGAGGGGAGATCAGTAGACAGCAGTGGAAACACATCAAACGGGGGTGGTGGATGTTGGAGGGGACAGCCATTCTGTAACTTTTTGATGGCATGTCTGGTAATAGCTTTTGTTCTTCCATGGTTTTTTCGTATAAACATTTTCTAGCAAGTGTCAAGAAAAAAAAGAAAAGAAAAGAAAAAGAAGAATTCTTCTTTTCCCATGGAACGGTTGACGTTTTCAACTTAACTTTGCTCTAATGCTGTTTAAACCCTGTACATCTTACAATCTTGCCAAACAGATGAATTTGTGGCTGGGGTTTTTTTTTTTTTTTTTTTTCAGTGCAGTTTCTGCAGCTTTTGCTTCGAGAATTGCTCACTTTTCTGTTAACAAAGCTTTTGTGAATGATTATGACCGATGTTTGAGTTTGTGATGATTTTGTAAGATG

mRNA sequence

ATGGATCGCGAAGAAAGGAATCGCCAAGATGGTGGTGATGTGTCGAGTCGAGTAGAAACAGCGGTGCCTAAACCCACAAATGATTTGGTTAATGGCGATAGGATCGAGGCTACCATTGTGATTATCCCAGAAAGCCTCGCCTCTGCTGGCGGCGACAAGGAAGCTCACGGCTTCGGTGGAGAAGCTATACCAAATGTTAATATAGAGACAAGGGAGGGTTCGAATCTGAACCCTTCAGAAACTGTACTGGTTATTGATTCGGATGGGGCTGCATCTAGAGGTGAAGACGGAGAATCGCTTGTGAAGAACAATGGAATGGGGTTGGGGAAGGATTTGGAAAAGTTGAATGTAAAATCAAAGATGTCAGAAGCGAAGGACAATCCGATGTTGATAAATGTAAAGCCAAAAGGGGGTAAAGGGTTTATTGATGACTGGAATGGAGAAAGGGTCTGTAGGATCTGCCATTTGGCGTCCAATCAAACCTCAGAGGCTAAAACTGGGACCAGTACTTCAGAATTGATTCAACTTGGTTGTGACTGCAAAGATGAGCTGGGAATTGCGCATGGTCACTGTGCTGAGGCATGGTTCAAGCTCAAAGGAAACAGAATGTGCGAAATTTGTGGTGAGACTGCTAAAAACGTTGAAGGAGTTGGGGACAACAGATTTATGGAAGAGTGGAACGAGGGGAGATCAGTAGACAGCAGTGGAAACACATCAAACGGGGGTGGTGGATGTTGGAGGGGACAGCCATTCTGTAACTTTTTGATGGCATGTCTGGTAATAGCTTTTGTTCTTCCATGGTTTTTTCGTATAAACATTTTCTAG

Coding sequence (CDS)

ATGGATCGCGAAGAAAGGAATCGCCAAGATGGTGGTGATGTGTCGAGTCGAGTAGAAACAGCGGTGCCTAAACCCACAAATGATTTGGTTAATGGCGATAGGATCGAGGCTACCATTGTGATTATCCCAGAAAGCCTCGCCTCTGCTGGCGGCGACAAGGAAGCTCACGGCTTCGGTGGAGAAGCTATACCAAATGTTAATATAGAGACAAGGGAGGGTTCGAATCTGAACCCTTCAGAAACTGTACTGGTTATTGATTCGGATGGGGCTGCATCTAGAGGTGAAGACGGAGAATCGCTTGTGAAGAACAATGGAATGGGGTTGGGGAAGGATTTGGAAAAGTTGAATGTAAAATCAAAGATGTCAGAAGCGAAGGACAATCCGATGTTGATAAATGTAAAGCCAAAAGGGGGTAAAGGGTTTATTGATGACTGGAATGGAGAAAGGGTCTGTAGGATCTGCCATTTGGCGTCCAATCAAACCTCAGAGGCTAAAACTGGGACCAGTACTTCAGAATTGATTCAACTTGGTTGTGACTGCAAAGATGAGCTGGGAATTGCGCATGGTCACTGTGCTGAGGCATGGTTCAAGCTCAAAGGAAACAGAATGTGCGAAATTTGTGGTGAGACTGCTAAAAACGTTGAAGGAGTTGGGGACAACAGATTTATGGAAGAGTGGAACGAGGGGAGATCAGTAGACAGCAGTGGAAACACATCAAACGGGGGTGGTGGATGTTGGAGGGGACAGCCATTCTGTAACTTTTTGATGGCATGTCTGGTAATAGCTTTTGTTCTTCCATGGTTTTTTCGTATAAACATTTTCTAG

Protein sequence

MDREERNRQDGGDVSSRVETAVPKPTNDLVNGDRIEATIVIIPESLASAGGDKEAHGFGGEAIPNVNIETREGSNLNPSETVLVIDSDGAASRGEDGESLVKNNGMGLGKDLEKLNVKSKMSEAKDNPMLINVKPKGGKGFIDDWNGERVCRICHLASNQTSEAKTGTSTSELIQLGCDCKDELGIAHGHCAEAWFKLKGNRMCEICGETAKNVEGVGDNRFMEEWNEGRSVDSSGNTSNGGGGCWRGQPFCNFLMACLVIAFVLPWFFRINIF*
Homology
[Close]
BLAST of Csa6G519670 vs. TrEMBL
Match: A0A0A0KHW6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G519670 PE=4 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 1.9e-159
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 1 

Query: 1   MDREERNRQDGGDVSSRVETAVPKPTNDLVNGDRIEATIVIIPESLASAGGDKEAHGFGG 60
           MDREERNRQDGGDVSSRVETAVPKPTNDLVNGDRIEATIVIIPESLASAGGDKEAHGFGG
Sbjct: 1   MDREERNRQDGGDVSSRVETAVPKPTNDLVNGDRIEATIVIIPESLASAGGDKEAHGFGG 60

Query: 61  EAIPNVNIETREGSNLNPSETVLVIDSDGAASRGEDGESLVKNNGMGLGKDLEKLNVKSK 120
           EAIPNVNIETREGSNLNPSETVLVIDSDGAASRGEDGESLVKNNGMGLGKDLEKLNVKSK
Sbjct: 61  EAIPNVNIETREGSNLNPSETVLVIDSDGAASRGEDGESLVKNNGMGLGKDLEKLNVKSK 120

Query: 121 MSEAKDNPMLINVKPKGGKGFIDDWNGERVCRICHLASNQTSEAKTGTSTSELIQLGCDC 180
           MSEAKDNPMLINVKPKGGKGFIDDWNGERVCRICHLASNQTSEAKTGTSTSELIQLGCDC
Sbjct: 121 MSEAKDNPMLINVKPKGGKGFIDDWNGERVCRICHLASNQTSEAKTGTSTSELIQLGCDC 180

Query: 181 KDELGIAHGHCAEAWFKLKGNRMCEICGETAKNVEGVGDNRFMEEWNEGRSVDSSGNTSN 240
           KDELGIAHGHCAEAWFKLKGNRMCEICGETAKNVEGVGDNRFMEEWNEGRSVDSSGNTSN
Sbjct: 181 KDELGIAHGHCAEAWFKLKGNRMCEICGETAKNVEGVGDNRFMEEWNEGRSVDSSGNTSN 240

Query: 241 GGGGCWRGQPFCNFLMACLVIAFVLPWFFRINIF 275
           GGGGCWRGQPFCNFLMACLVIAFVLPWFFRINIF
Sbjct: 241 GGGGCWRGQPFCNFLMACLVIAFVLPWFFRINIF 274

[Close]
BLAST of Csa6G519670 vs. TrEMBL
Match: A0A061GDI8_THECC (RING/FYVE/PHD zinc finger superfamily protein, putative OS=Theobroma cacao GN=TCM_029667 PE=4 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.3e-62
Identity = 126/249 (50.60%), Postives = 167/249 (67.07%), Query Frame = 1 

Query: 40  VIIPESLASAGG---DKEAHGFGGEAIPNVNIETREGSNLNPS------ETVLVIDSDGA 99
           V+  E L  AGG   D+E +  GG       ++     +LN S      ET++V+++  A
Sbjct: 116 VVSTEVLGEAGGEMFDQETNQMGGVGETTGQVDQGSSRSLNNSVDRFVVETIIVVNTQEA 175

Query: 100 ASRGEDGESLVKNNGMGLGKDLEKLNVKSKMSEAKDNPML-INVKPKGGKGFIDDWNGER 159
                     VK+NG+G  K + +   K+K++EA+D+ ++ I     GG+ + + W+GER
Sbjct: 176 TCVDGSNRLEVKDNGLGSSKVMTE-RPKTKVAEAEDSCVIDIKGSDGGGRQYKESWDGER 235

Query: 160 VCRICHLASNQTSEAKTGT----STSELIQLGCDCKDELGIAHGHCAEAWFKLKGNRMCE 219
           VCRICHL + Q+ E+   T    +T +LIQLGC CKDELGIAH HCAEAWFKLKGNRMCE
Sbjct: 236 VCRICHLTTEQSLESTDITFSTAATMDLIQLGCGCKDELGIAHSHCAEAWFKLKGNRMCE 295

Query: 220 ICGETAKNVEGVGDNRFMEEWNEGRSVDSSGNTSNGGGGCWRGQPFCNFLMACLVIAFVL 275
           ICG+TAKN+ GV DNRF+E+W++  S    G +S+ GGGCWRGQPFCNFLMACLVIAFVL
Sbjct: 296 ICGQTAKNITGVRDNRFIEDWHDQGSTSGGGISSDRGGGCWRGQPFCNFLMACLVIAFVL 355

[Close]
BLAST of Csa6G519670 vs. TrEMBL
Match: M5WUB0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009296mg PE=4 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 1.9e-61
Identity = 139/299 (46.49%), Postives = 181/299 (60.54%), Query Frame = 1 

Query: 1   MDREERNRQDGGDVSSRVETAVPKPTNDLVNGDRIEATIVI-IPESLASAGGDKEAHGFG 60
           MD    + Q   +V   V+    + + + V    +E  IVI   E+L    G K+A   G
Sbjct: 1   MDSGAHDLQGSSEVLGAVDQKTIEKSRNSVVWSVVETVIVIESEEALGKELGSKKAVVEG 60

Query: 61  GEAIPN-----------VNIETREGSNLNP--------SETVLVIDSDGAAS-RGEDGES 120
            E + +           V  E  +G+N N         SET++VI+SD AA   G++G  
Sbjct: 61  LEKLNDKEACDQPGSGVVPSEVEQGNNKNSNNLADGNVSETLVVINSDEAARVTGDNGRL 120

Query: 121 LVKNNGMGLGKDLEKLNVKSKMSEAKDNPMLINVKPKGGKGFIDDWNGERVCRICHLASN 180
           + K + +G  K +     K K+ +A+ +  +I+V+   GKGF D W+ ERVCRICHL+S 
Sbjct: 121 VTKVDELGSSK-VSVEESKKKVPQAERDSHVIDVRCGSGKGFGDKWDVERVCRICHLSSE 180

Query: 181 QTSEAKTGTSTS----ELIQLGCDCKDELGIAHGHCAEAWFKLKGNRMCEICGETAKNVE 240
           Q+ + K  T+ S    ELI LGC+C+DELG+AHGHCAEAWFKLKGNR CEICGETA NV 
Sbjct: 181 QSPDRKIETANSSTPTELIHLGCECRDELGVAHGHCAEAWFKLKGNRTCEICGETANNVT 240

Query: 241 GVGDNRFMEEWNEGRSVDSSGNTSNGGGGCWRGQPFCNFLMACLVIAFVLPWFFRINIF 275
           G GD+RFMEEWNE        N+ +  GGCWRGQPFCNFLMACLVIAFVLPWFFR+ +F
Sbjct: 241 GHGDSRFMEEWNEETLSGIENNSLDRNGGCWRGQPFCNFLMACLVIAFVLPWFFRVKMF 298

[Close]
BLAST of Csa6G519670 vs. TrEMBL
Match: F6HMA8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_10s0003g01500 PE=4 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 2.4e-61
Identity = 123/225 (54.67%), Postives = 155/225 (68.89%), Query Frame = 1 

Query: 60  GEAIPNVNIETREGSNLNP-SETVLVI---DSDGAASRGEDGESLVKNNGMGLGKDLEKL 119
           G+ +  V    RE S+  P  ETV++I   + D    RG  G S    +     +   + 
Sbjct: 12  GDKVDRVEGGIRESSSAEPVPETVIMITPAEPDAEERRGSSGGS---GSDKAKAEAEAEA 71

Query: 120 NVKSKMSEAKDNPMLINVK---PKGGKGFIDDWNGERVCRICHLASNQTSEAKTGT---S 179
             +SK S  + N  +++VK     GG G +++W+GERVCRICHL+S+  +E    T   +
Sbjct: 72  ETESKASVKEVNSCVVDVKCGGGGGGGGLVENWDGERVCRICHLSSDPAAEGSIATCRDA 131

Query: 180 TSELIQLGCDCKDELGIAHGHCAEAWFKLKGNRMCEICGETAKNVEGVGDNRFMEEWNEG 239
           +++LIQLGC CKDELGI+H HCAEAWFKLKGNRMCEICGETA NV+GV DNRFME+WNE 
Sbjct: 132 SADLIQLGCGCKDELGISHPHCAEAWFKLKGNRMCEICGETANNVKGVWDNRFMEDWNER 191

Query: 240 RSVDSSGNTSNGGGGCWRGQPFCNFLMACLVIAFVLPWFFRINIF 275
           R   SS N+S+ GGGCWRGQPFCNFLMACLVIAFVLPWFFR+N+F
Sbjct: 192 RYAGSSSNSSDRGGGCWRGQPFCNFLMACLVIAFVLPWFFRVNMF 233

[Close]
BLAST of Csa6G519670 vs. TrEMBL
Match: W9RP22_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_005813 PE=4 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 2.1e-60
Identity = 125/283 (44.17%), Postives = 172/283 (60.78%), Query Frame = 1 

Query: 1   MDREERNRQDGGDVSSRVETAVPKPTNDLVNGDRIEATIVIIPESLASAGGDKEAHGFGG 60
           MD EE + Q   +V SR +    + ++D V+G  +E    +  E  A    + +     G
Sbjct: 1   MDEEEHDPQSSNEVLSRDDRRAKENSSDFVDGSAVETITAVKKEERACHSEENKDLELRG 60

Query: 61  EAIPNVNIETREGSNLNPSETVLVIDSDGAASRGEDGESLVKNNGMGLGKDLEKLNV--- 120
             + + N    +G+  NP+ + + +  + +         LV  +   L   +++L +   
Sbjct: 61  CELGSTN-GVEQGTIANPTNSGVRVVVEASVVISPSEAPLVSGDNRVLNAKVDELGLSIM 120

Query: 121 -----KSKMSEAKDNPMLINVKPKGGKGFIDDWNGERVCRICHLASNQTSEAKTGT-STS 180
                KSKMSE++    +I++     K   D W+GER+CRICHL+S+Q+ + +T T S +
Sbjct: 121 SVEESKSKMSESEKQSCVIDISCGSAKSCGDKWDGERICRICHLSSDQSPDRRTTTTSVA 180

Query: 181 ELIQLGCDCKDELGIAHGHCAEAWFKLKGNRMCEICGETAKNVEGVGDNRFMEEWNEGRS 240
           +LI LGC CKD+L IAH  CAEAWFK+KGNRMCEICGETAKN+ GVGD RFMEEWNE R 
Sbjct: 181 DLIHLGCGCKDDLSIAHYQCAEAWFKVKGNRMCEICGETAKNITGVGDIRFMEEWNE-RF 240

Query: 241 VDSSGNTSNGGGGCWRGQPFCNFLMACLVIAFVLPWFFRINIF 275
             S   +S   GGCWRGQPFCNFLMACLV+AFVLPWFFR+N+F
Sbjct: 241 TRSGSMSSERSGGCWRGQPFCNFLMACLVVAFVLPWFFRVNMF 281

[Close]
BLAST of Csa6G519670 vs. TAIR10
Match: AT2G34200.1 (AT2G34200.1 RING/FYVE/PHD zinc finger superfamily protein)

HSP 1 Score: 120.2 bits (300), Expect = 2.0e-27
Identity = 64/126 (50.79%), Postives = 79/126 (62.70%), Query Frame = 1 

Query: 147 GERVCRICHLASNQTSEAKTGTST--SELIQLGCDCKDELGIAHGHCAEAWFKLKGNRMC 206
           GER+CRICH  S+Q+ EA     +   ELI++GC CK+ELG+AH HCAEAWFKL+GN +C
Sbjct: 98  GERICRICHFGSDQSPEASGDDKSVSPELIEIGCKCKNELGLAHFHCAEAWFKLRGNSVC 157

Query: 207 EICGETAKNVEGVGDNRFMEEWNEGRSVDSSGNTSNGGGGCWRGQPFCNFLMACLVIAFV 266
           EICG TAKNV      R ME+W+  R      NT +G     RGQ  C F++  L I  +
Sbjct: 158 EICGCTAKNV----TVRLMEDWSGERD-----NTLDGRRRRGRGQSCCIFMVFLLTI-LL 213

Query: 267 LPWFFR 271
           L WFF+
Sbjct: 218 LHWFFK 213

[Close]
BLAST of Csa6G519670 vs. TAIR10
Match: AT5G05830.1 (AT5G05830.1 RING/FYVE/PHD zinc finger superfamily protein)

HSP 1 Score: 119.0 bits (297), Expect = 4.6e-27
Identity = 67/188 (35.64%), Postives = 98/188 (52.13%), Query Frame = 1 

Query: 91  ASRGEDGESLVKNNGMGLGKDLEKLNVKSKMSEAKDNPMLINVKPKGGKGFIDDWNGERV 150
           +S G DG+   + +   + K+ E +  + + S A    + I+++    +  +     ER 
Sbjct: 19  SSSGCDGDGEPRRSSGAVEKEEEIVKDEERRSNASVCSVEIDLELGLPEKVVHLSQSERD 78

Query: 151 CRICHLASNQTSEAKTGTSTSELIQLGCDCKDELGIAHGHCAEAWFKLKGNRMCEICGET 210
           CRICH+ S   +  ++G      I+LGC CK +L  AH HCAE WFK+KGN++CE+CG  
Sbjct: 79  CRICHM-SLDAANLESGVP----IELGCSCKADLAAAHKHCAETWFKIKGNKICEVCGSI 138

Query: 211 AKNVEGVGDNRFMEEWNEGRSVD-----SSGNTSNGGGGCWRGQPFCNFLMACLVIAFVL 270
           A NV G  +    E  NE   V+     +SG     G   W+G  F NFL+AC+V AFV+
Sbjct: 139 AGNVVGSVEVESEESRNEANGVENLTLRTSGPRLVEGRSFWQGHRFLNFLLACMVFAFVI 198

Query: 271 PWFFRINI 274
            W F  N+
Sbjct: 199 SWLFHFNV 201

[Close]
BLAST of Csa6G519670 vs. TAIR10
Match: AT5G59000.1 (AT5G59000.1 RING/FYVE/PHD zinc finger superfamily protein)

HSP 1 Score: 104.0 bits (258), Expect = 1.5e-22
Identity = 55/153 (35.95%), Postives = 65/153 (42.48%), Query Frame = 1 

Query: 147 GERVCRICHLASNQTSEAKTGTSTSE--------------------------LIQLGCDC 206
           G R CRICHL      EA+      E                           +QLGC C
Sbjct: 77  GGRDCRICHLPLETNKEAEDEDEEEEDDSDDDEDEEDEEEEEEEEEEEYYGLPLQLGCSC 136

Query: 207 KDELGIAHGHCAEAWFKLKGNRMCEICGETAKNVEGVGDNRFMEEWNEGRSVDSSGNTSN 266
           K +LG+AH  CAE WFK+KGN  CEICG  A NV G   N         ++      T  
Sbjct: 137 KGDLGVAHSKCAETWFKIKGNMTCEICGAMALNVAGEQSNPESTASTHSQAAAGQSLTQT 196

Query: 267 GGGGCWRGQPFCNFLMACLVIAFVLPWFFRINI 274
              G W G+P  NFL+A +V AFV+ W F   +
Sbjct: 197 EPRGIWHGRPVMNFLLAAMVFAFVVSWLFHFKV 229

[Close]
BLAST of Csa6G519670 vs. TAIR10
Match: AT2G37950.1 (AT2G37950.1 RING/FYVE/PHD zinc finger superfamily protein)

HSP 1 Score: 100.5 bits (249), Expect = 1.7e-21
Identity = 51/133 (38.35%), Postives = 68/133 (51.13%), Query Frame = 1 

Query: 148 ERVCRICHLASNQTSEAKTGTSTSELIQLGCDCKDELGIAHGHCAEAWFKLKGNRMCEIC 207
           E+ CRICHL           TS    I+LGC CKD+L +AH  CAE WFK+KG++ CEIC
Sbjct: 81  EKDCRICHLGVE--------TSGGGAIELGCSCKDDLAVAHRQCAETWFKIKGDKTCEIC 140

Query: 208 GETAKNVEGVGDNRFMEEWNEGRSVDSSGNTSNGGGG-------CWRGQPFCNFLMACLV 267
              A+NV G   N  +    E R + +   T+ G GG        W+ Q   N ++AC+V
Sbjct: 141 QSVARNVGGA--NEMVGSTMEERELRNGEETAAGEGGGATVVENRWQPQRVVNLVLACMV 200

Query: 268 IAFVLPWFFRINI 274
             F + W F  ++
Sbjct: 201 FGFFISWIFHFHV 203

[Close]
BLAST of Csa6G519670 vs. TAIR10
Match: AT5G01070.2 (AT5G01070.2 RING/FYVE/PHD zinc finger superfamily protein)

HSP 1 Score: 96.3 bits (238), Expect = 3.2e-20
Identity = 55/134 (41.04%), Postives = 71/134 (52.99%), Query Frame = 1 

Query: 148 ERVCRICHLASNQTSEAKTGTSTSELIQLGCDCKDELGIAHGHCAEAWFKLKGNRMCEIC 207
           E+ CRICHL   ++S  + G    + + LGC CKD+LG  H  CA+ WFK+KGN+ CEIC
Sbjct: 75  EKDCRICHLGL-ESSRHECG----DPMVLGCSCKDDLGYVHKQCADTWFKIKGNKTCEIC 134

Query: 208 GETAKNVEGVGDNRFMEEWNEGRSVD-SSGNT-------SNGGGGCWRGQPFCNFLMACL 267
              A+N   V DN   +   E    D  +GNT        +      RG  F NFL+ C+
Sbjct: 135 RSIAQNFFKV-DNEIGQTTVETNVDDVEAGNTPTMVATSDSDDRRLCRGNRFLNFLLTCM 194

Query: 268 VIAFVLPWFFRINI 274
           V AFV+ WFF  N+
Sbjct: 195 VCAFVISWFFHFNL 202

[Close]
BLAST of Csa6G519670 vs. NCBI nr
Match: gi|778721140|ref|XP_011658254.1| (PREDICTED: uncharacterized protein LOC105435966 [Cucumis sativus])

HSP 1 Score: 569.7 bits (1467), Expect = 2.8e-159
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 1 

Query: 1   MDREERNRQDGGDVSSRVETAVPKPTNDLVNGDRIEATIVIIPESLASAGGDKEAHGFGG 60
           MDREERNRQDGGDVSSRVETAVPKPTNDLVNGDRIEATIVIIPESLASAGGDKEAHGFGG
Sbjct: 1   MDREERNRQDGGDVSSRVETAVPKPTNDLVNGDRIEATIVIIPESLASAGGDKEAHGFGG 60

Query: 61  EAIPNVNIETREGSNLNPSETVLVIDSDGAASRGEDGESLVKNNGMGLGKDLEKLNVKSK 120
           EAIPNVNIETREGSNLNPSETVLVIDSDGAASRGEDGESLVKNNGMGLGKDLEKLNVKSK
Sbjct: 61  EAIPNVNIETREGSNLNPSETVLVIDSDGAASRGEDGESLVKNNGMGLGKDLEKLNVKSK 120

Query: 121 MSEAKDNPMLINVKPKGGKGFIDDWNGERVCRICHLASNQTSEAKTGTSTSELIQLGCDC 180
           MSEAKDNPMLINVKPKGGKGFIDDWNGERVCRICHLASNQTSEAKTGTSTSELIQLGCDC
Sbjct: 121 MSEAKDNPMLINVKPKGGKGFIDDWNGERVCRICHLASNQTSEAKTGTSTSELIQLGCDC 180

Query: 181 KDELGIAHGHCAEAWFKLKGNRMCEICGETAKNVEGVGDNRFMEEWNEGRSVDSSGNTSN 240
           KDELGIAHGHCAEAWFKLKGNRMCEICGETAKNVEGVGDNRFMEEWNEGRSVDSSGNTSN
Sbjct: 181 KDELGIAHGHCAEAWFKLKGNRMCEICGETAKNVEGVGDNRFMEEWNEGRSVDSSGNTSN 240

Query: 241 GGGGCWRGQPFCNFLMACLVIAFVLPWFFRINIF 275
           GGGGCWRGQPFCNFLMACLVIAFVLPWFFRINIF
Sbjct: 241 GGGGCWRGQPFCNFLMACLVIAFVLPWFFRINIF 274

[Close]
BLAST of Csa6G519670 vs. NCBI nr
Match: gi|659078443|ref|XP_008439727.1| (PREDICTED: uncharacterized protein LOC103484442 [Cucumis melo])

HSP 1 Score: 544.7 bits (1402), Expect = 9.5e-152
Identity = 262/274 (95.62%), Postives = 266/274 (97.08%), Query Frame = 1 

Query: 1   MDREERNRQDGGDVSSRVETAVPKPTNDLVNGDRIEATIVIIPESLASAGGDKEAHGFGG 60
           MDREERNRQDGGDVSSRVET VPKPTNDLVNGDRIEATIVIIPES ASA GDKEAHGFGG
Sbjct: 1   MDREERNRQDGGDVSSRVETVVPKPTNDLVNGDRIEATIVIIPESAASACGDKEAHGFGG 60

Query: 61  EAIPNVNIETREGSNLNPSETVLVIDSDGAASRGEDGESLVKNNGMGLGKDLEKLNVKSK 120
           EAIPNVNI+TREGSNLNPSETVLVIDSDGAASRGEDGESL+KN+ MGL KDLEKLNVKSK
Sbjct: 61  EAIPNVNIDTREGSNLNPSETVLVIDSDGAASRGEDGESLMKNSEMGLRKDLEKLNVKSK 120

Query: 121 MSEAKDNPMLINVKPKGGKGFIDDWNGERVCRICHLASNQTSEAKTGTSTSELIQLGCDC 180
           MSE KDNPMLINVKPKGGKGFIDDWNGERVCRICHLASNQ SEAKTGTSTSELIQLGCDC
Sbjct: 121 MSETKDNPMLINVKPKGGKGFIDDWNGERVCRICHLASNQASEAKTGTSTSELIQLGCDC 180

Query: 181 KDELGIAHGHCAEAWFKLKGNRMCEICGETAKNVEGVGDNRFMEEWNEGRSVDSSGNTSN 240
           KDELGIAHGHCAEAWFKLKGNRMCEICGETAKNVEGVGDNRFMEEWNEGRSVDS+GNTSN
Sbjct: 181 KDELGIAHGHCAEAWFKLKGNRMCEICGETAKNVEGVGDNRFMEEWNEGRSVDSNGNTSN 240

Query: 241 GGGGCWRGQPFCNFLMACLVIAFVLPWFFRINIF 275
            GGGCWRGQPFCNFLMACLVIAFVLPWFFRINIF
Sbjct: 241 RGGGCWRGQPFCNFLMACLVIAFVLPWFFRINIF 274

[Close]
BLAST of Csa6G519670 vs. NCBI nr
Match: gi|470115560|ref|XP_004293966.1| (PREDICTED: uncharacterized protein LOC101301561 [Fragaria vesca subsp. vesca])

HSP 1 Score: 249.6 bits (636), Expect = 6.3e-63
Identity = 132/277 (47.65%), Postives = 170/277 (61.37%), Query Frame = 1 

Query: 2   DREERNRQDGGDVSSRVETAVPKPTNDLVNGDRIEATIVIIPESLASAGGDKEAHGFGGE 61
           D+E   +++  DVS   E    K +++L NG+   + +VI     + A GD    G  G+
Sbjct: 75  DKEACEQRNSSDVSGHAELGTSKNSSNLANGNVSGSVVVIDSGEASRATGDDGRLGVEGD 134

Query: 62  AIPNVNIETREGSNLNPSETVLVIDSDGAASRGEDGESLVKNNGMGLGKDLEKLNVKSKM 121
            + +  ++  +         + VI+              +K + MG  K  E    K K+
Sbjct: 135 ELGSNKVDEPKMKVPEKESDLHVIN--------------IKCDEMGSSKVEES---KMKV 194

Query: 122 SEAKDNPMLINVKPKGGKGFIDDWNGERVCRICHLASNQTSEAKTGTST----SELIQLG 181
            EA+ +  +IN+K    KGF D+W GE VCRICHL+S+Q+ + KT +S+     +LI LG
Sbjct: 195 PEAERDSHVINIKCGSRKGFGDNWEGESVCRICHLSSDQSPDRKTESSSYSVSMDLIHLG 254

Query: 182 CDCKDELGIAHGHCAEAWFKLKGNRMCEICGETAKNVEGVGDNRFMEEWNEGRSVDSSGN 241
           C CKDELGIAH HCAEAWFKLKGNRMCEICGE AKNV G+GDNRF+EEWNE R      N
Sbjct: 255 CGCKDELGIAHAHCAEAWFKLKGNRMCEICGENAKNVTGLGDNRFLEEWNERRFSGLGAN 314

Query: 242 TSNGGGGCWRGQPFCNFLMACLVIAFVLPWFFRINIF 275
           TS    GCWRGQPFCNFLMACLVIAFVLPWFFR+N+F
Sbjct: 315 TSERNVGCWRGQPFCNFLMACLVIAFVLPWFFRVNMF 334

[Close]
BLAST of Csa6G519670 vs. NCBI nr
Match: gi|590623497|ref|XP_007025338.1| (RING/FYVE/PHD zinc finger superfamily protein, putative [Theobroma cacao])

HSP 1 Score: 248.1 bits (632), Expect = 1.8e-62
Identity = 126/249 (50.60%), Postives = 167/249 (67.07%), Query Frame = 1 

Query: 40  VIIPESLASAGG---DKEAHGFGGEAIPNVNIETREGSNLNPS------ETVLVIDSDGA 99
           V+  E L  AGG   D+E +  GG       ++     +LN S      ET++V+++  A
Sbjct: 116 VVSTEVLGEAGGEMFDQETNQMGGVGETTGQVDQGSSRSLNNSVDRFVVETIIVVNTQEA 175

Query: 100 ASRGEDGESLVKNNGMGLGKDLEKLNVKSKMSEAKDNPML-INVKPKGGKGFIDDWNGER 159
                     VK+NG+G  K + +   K+K++EA+D+ ++ I     GG+ + + W+GER
Sbjct: 176 TCVDGSNRLEVKDNGLGSSKVMTE-RPKTKVAEAEDSCVIDIKGSDGGGRQYKESWDGER 235

Query: 160 VCRICHLASNQTSEAKTGT----STSELIQLGCDCKDELGIAHGHCAEAWFKLKGNRMCE 219
           VCRICHL + Q+ E+   T    +T +LIQLGC CKDELGIAH HCAEAWFKLKGNRMCE
Sbjct: 236 VCRICHLTTEQSLESTDITFSTAATMDLIQLGCGCKDELGIAHSHCAEAWFKLKGNRMCE 295

Query: 220 ICGETAKNVEGVGDNRFMEEWNEGRSVDSSGNTSNGGGGCWRGQPFCNFLMACLVIAFVL 275
           ICG+TAKN+ GV DNRF+E+W++  S    G +S+ GGGCWRGQPFCNFLMACLVIAFVL
Sbjct: 296 ICGQTAKNITGVRDNRFIEDWHDQGSTSGGGISSDRGGGCWRGQPFCNFLMACLVIAFVL 355

[Close]
BLAST of Csa6G519670 vs. NCBI nr
Match: gi|645237675|ref|XP_008225316.1| (PREDICTED: uncharacterized protein LOC103324972 [Prunus mume])

HSP 1 Score: 247.3 bits (630), Expect = 3.1e-62
Identity = 129/234 (55.13%), Postives = 162/234 (69.23%), Query Frame = 1 

Query: 52  DKEAHGFGGEAIPNVNIE---TREGSNL---NPSETVLVIDSDGAAS-RGEDGESLVKNN 111
           DKEA    G  +    +E    +  +NL   N SET++VI+SD AA   G++G  + K +
Sbjct: 78  DKEACDQPGSGVVPSEVEQGNNKNSNNLADGNVSETLVVINSDEAARVTGDNGRLVTKVD 137

Query: 112 GMGLGKDLEKLNVKSKMSEAKDNPMLINVKPKGGKGFIDDWNGERVCRICHLASNQTSEA 171
            +G  K L + + K  + +A+ +  +I+V+   GKGF D W+GERVCRICHL+S Q+ + 
Sbjct: 138 ELGSSKVLVEES-KKMVPQAEKDSHVIDVRCGSGKGFGDKWDGERVCRICHLSSEQSPDR 197

Query: 172 KTGTSTS----ELIQLGCDCKDELGIAHGHCAEAWFKLKGNRMCEICGETAKNVEGVGDN 231
           K  T+ S    ELI LGC+C+DELG+AHGHCAEAWFKLKGNR CEICGETA NV G GD+
Sbjct: 198 KIETANSSTPTELIHLGCECRDELGVAHGHCAEAWFKLKGNRTCEICGETANNVTGHGDS 257

Query: 232 RFMEEWNEGRSVDSSGNTSNGGGGCWRGQPFCNFLMACLVIAFVLPWFFRINIF 275
           RFMEEWNE        N+S+  GGCWRGQPFCNFLMACLVIAFVLPWFFR N+F
Sbjct: 258 RFMEEWNEETLSGIENNSSDRNGGCWRGQPFCNFLMACLVIAFVLPWFFRGNMF 310

The following BLAST results are available for this feature:
BLAST of Csa6G519670 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KHW6_CUCSA1.9e-159100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_6G519670 PE=4 SV=1[more]
A0A061GDI8_THECC1.3e-6250.60RING/FYVE/PHD zinc finger superfamily protein, putative OS=Theobroma cacao GN=TC... [more]
M5WUB0_PRUPE1.9e-6146.49Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009296mg PE=4 SV=1[more]
F6HMA8_VITVI2.4e-6154.67Putative uncharacterized protein OS=Vitis vinifera GN=VIT_10s0003g01500 PE=4 SV=... [more]
W9RP22_9ROSA2.1e-6044.17Uncharacterized protein OS=Morus notabilis GN=L484_005813 PE=4 SV=1[more]
BLAST of Csa6G519670 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G34200.12.0e-2750.79 RING/FYVE/PHD zinc finger superfamily protein[more]
AT5G05830.14.6e-2735.64 RING/FYVE/PHD zinc finger superfamily protein[more]
AT5G59000.11.5e-2235.95 RING/FYVE/PHD zinc finger superfamily protein[more]
AT2G37950.11.7e-2138.35 RING/FYVE/PHD zinc finger superfamily protein[more]
AT5G01070.23.2e-2041.04 RING/FYVE/PHD zinc finger superfamily protein[more]
BLAST of Csa6G519670 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778721140|ref|XP_011658254.1|2.8e-159100.00PREDICTED: uncharacterized protein LOC105435966 [Cucumis sativus][more]
gi|659078443|ref|XP_008439727.1|9.5e-15295.62PREDICTED: uncharacterized protein LOC103484442 [Cucumis melo][more]
gi|470115560|ref|XP_004293966.1|6.3e-6347.65PREDICTED: uncharacterized protein LOC101301561 [Fragaria vesca subsp. vesca][more]
gi|590623497|ref|XP_007025338.1|1.8e-6250.60RING/FYVE/PHD zinc finger superfamily protein, putative [Theobroma cacao][more]
gi|645237675|ref|XP_008225316.1|3.1e-6255.13PREDICTED: uncharacterized protein LOC103324972 [Prunus mume][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR011016Znf_RING-CH
IPR013083Znf_RING/FYVE/PHD
Vocabulary: Molecular Function
TermDefinition
GO:0008270zinc ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0042254 ribosome biogenesis
biological_process GO:0001510 RNA methylation
biological_process GO:0006412 translation
cellular_component GO:0005575 cellular_component
cellular_component GO:0022625 cytosolic large ribosomal subunit
cellular_component GO:0016020 membrane
cellular_component GO:0005773 vacuole
cellular_component GO:0005840 ribosome
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003735 structural constituent of ribosome
Unigene
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU096048cucumber EST collection version 3.0transcribed_cluster
CU101998cucumber EST collection version 3.0transcribed_cluster
CU128079cucumber EST collection version 3.0transcribed_cluster
CU129847cucumber EST collection version 3.0transcribed_cluster
CU156918cucumber EST collection version 3.0transcribed_cluster
CU158426cucumber EST collection version 3.0transcribed_cluster
CU161978cucumber EST collection version 3.0transcribed_cluster
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa6G519670.1Csa6G519670.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU161978CU161978transcribed_cluster
CU129847CU129847transcribed_cluster
CU128079CU128079transcribed_cluster
CU101998CU101998transcribed_cluster
CU096048CU096048transcribed_cluster
CU156918CU156918transcribed_cluster
CU158426CU158426transcribed_cluster


InterPro
Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011016Zinc finger, RING-CH-typePFAMPF12906RINGvcoord: 151..207
score: 7.
IPR011016Zinc finger, RING-CH-typeSMARTSM00744ringv_2coord: 150..208
score: 2.0
IPR011016Zinc finger, RING-CH-typePROFILEPS51292ZF_RING_CHcoord: 143..214
score: 17
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 147..210
score: 2.2
NoneNo IPR availablePANTHERPTHR23012:SF76SUBFAMILY NOT NAMEDcoord: 74..274
score: 8.3
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 147..210
score: 3.3

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Csa6G519670Cla009703Watermelon (97103) v1cuwmB500
Csa6G519670Cla002248Watermelon (97103) v1cuwmB491
Csa6G519670MELO3C007205Melon (DHL92) v3.5.1cumeB507
Csa6G519670ClCG01G023830Watermelon (Charleston Gray)cuwcgB432
Csa6G519670Lsi01G002310Bottle gourd (USVL1VR-Ls)culsiB434
Csa6G519670MELO3C007205.2Melon (DHL92) v3.6.1cumedB486
Csa6G519670Cla97C01G023790Watermelon (97103) v2cuwmbB412
Csa6G519670Bhi03G000217Wax gourdcuwgoB628
Csa6G519670Bhi05G001671Wax gourdcuwgoB565
Csa6G519670CSPI06G35470Wild cucumber (PI 183967)cpicuB313
Csa6G519670Cucsa.047050Cucumber (Gy14) v1cgycuB027
Csa6G519670CmaCh07G001770Cucurbita maxima (Rimu)cmacuB877
Csa6G519670CmaCh03G013290Cucurbita maxima (Rimu)cmacuB678
Csa6G519670CmoCh03G013300Cucurbita moschata (Rifu)cmocuB667
Csa6G519670CmoCh11G019800Cucurbita moschata (Rifu)cmocuB127
Csa6G519670CmoCh07G001720Cucurbita moschata (Rifu)cmocuB865
Csa6G519670Cp4.1LG19g06780Cucurbita pepo (Zucchini)cpecuB503
Csa6G519670Cp4.1LG10g05540Cucurbita pepo (Zucchini)cpecuB087
Csa6G519670CsGy6G034980Cucumber (Gy14) v2cgybcuB281
Csa6G519670Carg15352Silver-seed gourdcarcuB0578
Csa6G519670Carg23115Silver-seed gourdcarcuB0972
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Csa6G519670Watermelon (97103) v2cuwmbB453
Csa6G519670Wax gourdcuwgoB637
Csa6G519670Cucumber (Chinese Long) v2cucuB038
Csa6G519670Cucumber (Gy14) v1cgycuB316
Csa6G519670Cucurbita maxima (Rimu)cmacuB229
Csa6G519670Cucurbita maxima (Rimu)cmacuB422
Csa6G519670Cucurbita maxima (Rimu)cmacuB526
Csa6G519670Cucurbita moschata (Rifu)cmocuB215
Csa6G519670Cucurbita moschata (Rifu)cmocuB412
Csa6G519670Melon (DHL92) v3.5.1cumeB458
Csa6G519670Watermelon (Charleston Gray)cuwcgB462
Csa6G519670Watermelon (Charleston Gray)cuwcgB493
Csa6G519670Watermelon (97103) v1cuwmB480
Csa6G519670Cucurbita pepo (Zucchini)cpecuB051
Csa6G519670Cucurbita pepo (Zucchini)cpecuB254
Csa6G519670Cucurbita pepo (Zucchini)cpecuB525
Csa6G519670Bottle gourd (USVL1VR-Ls)culsiB438
Csa6G519670Cucumber (Gy14) v2cgybcuB043
Csa6G519670Melon (DHL92) v3.6.1cumedB438
Csa6G519670Silver-seed gourdcarcuB0169
Csa6G519670Silver-seed gourdcarcuB0816