Csa5G647400 (gene) Cucumber (Chinese Long) v2

BLAST of Csa5G647400 vs. Swiss-Prot
Match: ENG2_SCHPO (Putative endo-1,3(4)-beta-glucanase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=eng2 PE=3 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 2.6e-41
Identity = 175/690 (25.36%), Postives = 294/690 (42.61%), Query Frame = 1

Query: 31  PETTSTAVPDPGKFFSPNLLSSPLPTNSFFQNFVLNNGDLPEYIHPYLIRTANSS----L 90
           P   S A P P    + NL   P+ TN F+ N  +     P + HPY +   N S    L
Sbjct: 14  PVFPSRAHPSPPLGLTSNLF--PIQTNKFYGNLYIGTRHNPSWSHPYSVTWLNGSSYYGL 73

Query: 91  SVSY----------------------PSRISNSSITQLQFFPDLVIS-------SPKKTF 150
           ++S+                      P+ + +  I+  +F    ++S       S + T 
Sbjct: 74  AISHIDDSQRVFGPDPESVPCQYYFNPAGLYSIIISAREFASGNLLSLDQSRHFSIQATL 133

Query: 151 NTTHFISSFSDLGVDLDIGVFRFHLVRGSPYLTFSVLKTSSVLISTSNGVRSVDSYEDYT 210
           + T   S    L +   +G    +    +P    S+L +S   I+ S G           
Sbjct: 134 SATTSGSGTIILPIVAGMGFVSGYYTNLTPVFNSSILFSSITKINFSRGY---------- 193

Query: 211 KHIIRLNNGRSWVLY-----SSSAIYLVKSKSNQIVTSGGFIGVIRVAVLPDSAVES--- 270
           K+ I+L +G+ W LY     SSS   L  + ++Q+ TS  F G+I++  +P+ +V +   
Sbjct: 194 KYRIQLTDGKIWFLYAFPTSSSSTFNLTLASNSQLTTSTKFTGLIQICKVPNESVNNSYP 253

Query: 271 EKILDRYSGCYPVS-GFVKLLGGFGFEYKWQKKGSGGL-----LMLAHTLHREILPRD-Q 330
           + I D  +G Y  S      + G   EY W +  + G      LM A   H +    D Q
Sbjct: 254 DTIYDASAGVYTTSISLSAQVSGTTGEY-WFRFATAGYTNLNPLMFALPHHMQSFGSDTQ 313

Query: 331 TVLQNIRYSSID-GDLLGVVGDSWDL-------KFNPIPITWHSINGIDSKFFPEIVAAL 390
                +  +S   G +      +W L       +   +PI W   NG  + + P  +AA+
Sbjct: 314 AYKTGLGLASTTMGIMFAYATKTWHLIEKNLPTQVGFLPIPW---NGGSNTYSPTALAAI 373

Query: 391 KRDVAT------LNATELSSMPASYFYGKLLARAARLALIAEEVNGGAGVIPAVVKFLKN 450
           +   AT      +NA+ L SM   Y  GK++A  A++ L+A  + G + +    +  LK 
Sbjct: 374 RAACATDINFDVVNASNLDSM---YTSGKIVAMYAQVCLVASRILGDSTLTNTGLTKLKQ 433

Query: 451 GIQPWLIGKFPKNG----FLYERKWAGLVTKNGATSTTEDFGFGIYNDHHFHLGYFVYSI 510
            +      +F  N      +Y+  + G+++  G +S   D+G   YNDHHFH GY +Y+ 
Sbjct: 434 AM-----ARFTTNTQMYPLVYDTTYKGIISTAGYSSPLADYGNTYYNDHHFHWGYHIYAC 493

Query: 511 AVLAKLDPNW-GKQYKPQAYALLYDYMNFRPKKSQFSIPFRNFDFWKLHSWAAGLTEFPD 570
           AV+  LDP+W          ALL D  N     + F++ FRNFD++  HSWA G+ E  D
Sbjct: 494 AVIGLLDPSWLVNDNIRYVNALLRDSANPSESDTYFAM-FRNFDWFVGHSWATGIFESGD 553

Query: 571 GRNQESTTEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEITATQTWWHVKRENNGIYDK 630
           G+++EST+E  N  YA  L G+  +D  L    + + A    +  T+ ++    +     
Sbjct: 554 GKDEESTSEDFNFLYATKLWGMVRNDTVLINRANLMLAVLKNSLNTYIYMTPTTS--VQP 613

Query: 631 GFTEENRMVGILWSAARESRLWFAPAEWRECRVGIQVLPVLPVTERVFTDVGFVKEVVEW 654
                N + GI +    +   +F+  E+  C+ GI ++P  P++  + +     ++    
Sbjct: 614 SQILGNYVTGITFMNKVDYATYFSAEEY--CKQGIHMIPTTPISGYLRSPSYVQQDWNAK 672

BLAST of Csa5G647400 vs. Swiss-Prot
Match: ENG1_ARTBC (Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) GN=ARB_01444 PE=1 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 6.0e-38
Identity = 152/508 (29.92%), Postives = 231/508 (45.47%), Query Frame = 1

Query: 178 KHIIRLNNGRSWVLY---SSSAIYLVKSKSNQIVTSG-GFIGVIRVAVLPDSAVESEKIL 237
           K+ I L + ++W+LY    + A   +K + N++++   GF GVI+VA  P SA E E I 
Sbjct: 381 KYKITLEDDKNWLLYVIPENGADPKLKLEGNKLISGPTGFKGVIQVAKNP-SAEEGEGIY 440

Query: 238 DRYSGCYP----VSGFVKLLGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQT--VLQN 297
           D+ +G Y     +SG V   G   +++ ++K G G  L++    H      D T    +N
Sbjct: 441 DKSAGSYATDIKISGSVGTDGTGTYKFSFEKAGKGAPLVMYALPHHVESFDDATKNTKKN 500

Query: 298 IRYSSID-GDLLGVVGDSWDLKFNPIPIT-----WHSINGIDSKFFPEIVAALKRDVATL 357
           ++ S+   G     VGDSW +    +P++     W    G  S+    +    K  +  +
Sbjct: 501 MKLSTTTKGMATACVGDSWTMVEGNLPLSMDFAPWKP--GSSSQV--TLSEGAKNAIKAV 560

Query: 358 NATELS-------SMPASYFYGKLLARAARLALIAEEVNGGAGVIPAVVKFLKNGIQPWL 417
              ELS       ++ + YF GK L + A      +E+ G        +  LK   + ++
Sbjct: 561 AGNELSQDMELQTNLNSMYFSGKGLNKFAGAIYTVQELVGDKAAASGPLNSLKESFKRFV 620

Query: 418 IGKFPKNGFLYERKWAGLVTKNGATS--TTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDP 477
             K  +   +Y+  W G+V+        T  DFG  +YNDHHFH GYF+ + A+L KLDP
Sbjct: 621 DNK-QQIPLVYDNVWKGVVSSGTYEKGDTGLDFGNTLYNDHHFHYGYFILTAAILGKLDP 680

Query: 478 NWGKQYKPQAYALLYDYMNFRPKKSQFSIPF-RNFDFWKLHSWAAGLTEFPDGRNQESTT 537
            W    K     L+ D  N       F  PF R FD++  HSWA GL E  DG++QEST+
Sbjct: 681 AWLDANKAYVNMLVRDSGNSVDNDEHF--PFSRAFDWYHGHSWAKGLFESSDGKDQESTS 740

Query: 538 EAVNAYYAAALMGLAYDDESLTAAGSTLTAAEITATQTWWHVKRENNGIYDKGFTEENRM 597
           E     YA  + G    D+S+ A G+ +          ++ +K +N     K F   N++
Sbjct: 741 EDTMYAYAIKMWGKTSGDKSMEARGNLMLGILARTLNNYFLMKNDNVN-QPKNFI-GNKV 800

Query: 598 VGILWSAARESRLWF-APAEWRECRVGIQVLPVLPVTERVFTDVGFVKEVVEWVQ----- 654
            GIL+    +   +F A  E+ +   GI +LP+LP          FVKE  EW       
Sbjct: 801 TGILFENKIDHTTYFGANLEYIQ---GIHMLPLLP-NSAYTRSAEFVKE--EWEAMFASG 860

BLAST of Csa5G647400 vs. Swiss-Prot
Match: ENG2_YEAST (Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ACF2 PE=1 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 2.5e-36
Identity = 166/663 (25.04%), Postives = 291/663 (43.89%), Query Frame = 1

Query: 53  PLPTNSFFQNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRISNSSITQLQFFPDLVISS 112
           PL TN F+ N +L++   P + HPY I  +         +   N ++   + F D   + 
Sbjct: 110 PLETNKFYTNMLLDDNTQPIWTHPYSIWFSRDPELFGLAA---NHTLASQRVF-DTTTNP 169

Query: 113 PKKTFNTTH----------FISS------FSDLG-------VDLDIGVF-RFHLVRGSPY 172
           P+  FN T+          F+SS      F D+        + L    F  F LV+G  +
Sbjct: 170 PRFYFNPTNIKSFVFKAREFVSSNDIKLEFRDMKHMSMCLLMSLSSSQFIEFPLVQGMGF 229

Query: 173 LTFSVLKTSSVLISTSNGVRSVDSY---EDYTKHIIRLNNGRSWVLYSSSAIYLVKSK-- 232
           +T ++       + ++ G RS++     E Y K+ I+L N R+W+LY +S  Y       
Sbjct: 230 VT-AIYHDLGFELRSAVGFRSLERISVNERYGKYNIQLENNRNWILYLTSPDYSFPQDFQ 289

Query: 233 -----SNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCYPV----SGFVKLLGGFGF 292
                SN I++S    G+I        + +S   +D  +GCYPV    SG         +
Sbjct: 290 ISLLDSNTIISSHKINGLIC-----QLSADSVPSIDMAAGCYPVYCDLSGQTVDEHFTNY 349

Query: 293 EYKWQKKG---SGGLLMLAHTLHREILPRD--QTVLQNIRYSSIDGDLLGVVGDSWDLK- 352
            + +   G   SG  LM A   H+     +  +  + +   S++ G + G + +S+D++ 
Sbjct: 350 RFNYTVAGYSQSGTTLMYALPHHKAAFTPEMQEREIASSLDSTVKGLMTGYLTNSFDMQV 409

Query: 353 -------FNPIPITWHSINGIDSKFFPEIVAALKRDVATLNATELSSMPASYFYGKLLAR 412
                  F P+ ++ +       +   +I  A  ++V   +  + S++ + YF GK+LA+
Sbjct: 410 QVPQELGFEPVALSLNKKADYSQEKLSKIREAAVQEVQLSDPQQESNIDSMYFSGKILAK 469

Query: 413 AARLALIAEEVNGGAGVIPAVVKFLKNGIQPWLIGK--FPKNGFLYERKWAGLVTKNGAT 472
            A +  +   +     +   ++  L   ++ ++  +   P N   Y+  W G+++     
Sbjct: 470 YAWILYVTHYILHDENLTKELLSKLTIAMERFISNQQVLPLN---YDVSWKGIISSG--- 529

Query: 473 STTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPN--------WGKQYKPQAYALLYDYMN 532
           S+++DFG   YNDHHFH  Y V + A+++ +D +        W +  +     L+ DY  
Sbjct: 530 SSSQDFGNSYYNDHHFHYSYHVITAAIISLVDSDLSGVTNNSWLENNRDWVECLIRDYSG 589

Query: 533 FRPKKSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTTEAVNAYYAAALMGLAYDDES 592
                  F   FR+FD++  HSWA GL    DG+++EST+E VN+ YA  L GL   +  
Sbjct: 590 VDNDDPYFP-QFRSFDWFNGHSWAKGLFPSGDGKDEESTSEDVNSCYAIKLWGLVTGNSK 649

Query: 593 LTAAGSTLTAAEITATQTWWHVKRENNGIYDKGFTEENRMVGILWSAARESRLWFAPAEW 652
           LT   +          Q+++ +   NN +  K F   N++ GIL+    +   +F   E 
Sbjct: 650 LTDIANLQLGIMRNVFQSYF-LYESNNTVQPKEFI-GNKVSGILFENKIDHATYFG-MEP 709

BLAST of Csa5G647400 vs. Swiss-Prot
Match: ENG1_YEAST (Endo-1,3(4)-beta-glucanase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DSE4 PE=1 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 4.8e-35
Identity = 168/686 (24.49%), Postives = 299/686 (43.59%), Query Frame = 1

Query: 31   PETTSTAVPDPGKFFSPNLLSSPLPTNSFFQNFVLNNGDLPEYIHPYLIRTANSSLSVSY 90
            P T     P+P           P+ TN F+ N ++ + + P +++PY +    SS   SY
Sbjct: 411  PPTVFDRSPNPMSLADGVSNDGPIQTNKFYTNLIVGSQESPAFVYPYSLWKYTSS---SY 470

Query: 91   PSRISNSSITQLQF----------FPDLVISSPKKTFNTTHFISSFSDLGVDLDIGVFR- 150
               + ++++ Q  +          +    +      F+ ++F SS +    ++ +   R 
Sbjct: 471  GFAVQHTTVDQYSYGGYDSSGNAEYLVNPLGIAHVVFSASNFDSSMTMQVDEMTLSSTRV 530

Query: 151  -------------FHLVRGSPYLTFSVLKTSSVLISTSNGVRSVDSY------EDYTKHI 210
                           LV+G  + T     + +  I +S G  ++ S       +   K+ 
Sbjct: 531  VLSESNDSSNYLEIPLVQGMGFATGIYHGSLNAKIGSSVGFNTIVSESSSNLAQGILKYR 590

Query: 211  IRLNNGRSWVLY-------SSSAIYLVKSKSNQIVTSGGFIG-VIRVAVLPDSAVESEKI 270
            I L NG +W+ Y       +S+   L  S   +I  S    G +I++AV P S  + E  
Sbjct: 591  ITLLNGVTWLCYVIGPDDLTSTDFSLEVSSEYEIKASASVDGLIIQLAVAP-SETDYEVF 650

Query: 271  LDRYSGCYPVSGFVKLLG-------GFGFEYKWQKKGSGGLLMLAHTLHREILPRD--QT 330
             D+ +G Y V+ F KL G        + F Y  Q + + G  M+    H E    D  Q 
Sbjct: 651  YDQAAGMY-VTNF-KLQGVSDGSTATYEFSYTTQGESASGSTMIFALPHHESSFSDIMQD 710

Query: 331  VLQNIRYSS-----IDGDLLGVVGDSWDLKFNPIPITWHSINGID----SKFFPEIVAAL 390
                I+ +S     ++G L   +  S  L      + W S  G +    SK   +++A +
Sbjct: 711  YYTGIQLASTTKGVMNGYLTTSLQFSTSLNRQISWLPWSSQLGSNLLEYSKEQLQLLAEV 770

Query: 391  KRDVATLNATELSSMPASYFYGKLLARAARLALIAEEVNGGAGVIPAVVKFLKNGIQPWL 450
                  ++ +E  S   +Y+ GK++ + + + L   E+        + ++ +K+     L
Sbjct: 771  ANSELQVSISESISGLNTYYLGKVIDKYSYILLTVSEIIQDEASTKSTLENIKSAFDILL 830

Query: 451  IGK--FPKNGFLYERKWAGLVTKN--GATSTTEDFGFGIYNDHHFHLGYFVYSIAVL--- 510
              +  +P    +Y+ K+ GLV+    G+TST  DFG   YNDHHFH GY +++ AV+   
Sbjct: 831  QNEQTYP---LIYDTKFNGLVSSGDWGSTSTQYDFGNTYYNDHHFHYGYIIHAAAVIGYV 890

Query: 511  -AKLDPNWGKQYKPQAYALLYDYMNFRPKKSQFSIPFRNFDFWKLHSWAAGLTEFPDGRN 570
             +KL+  W    K    +L+ D  N   +K ++    R FD++  HSWAAGL E  +G+N
Sbjct: 891  DSKLNGTWAADNKDWVNSLVRDVAN-PSEKDEYFAQSRMFDWFNGHSWAAGLYENGNGKN 950

Query: 571  QESTTEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEITATQTWWHVKRENNGIYDKGFT 630
            +ES++E  N  YA  L G    D+S+   G  + +    A   +++ + +N    +    
Sbjct: 951  EESSSEDYNFAYAMKLWGATIGDQSMELRGDLMISIMKDAMNDYFYYQNDNT--VEPEEI 1010

Query: 631  EENRMVGILWSAARESRLWFAPAEWRECRVGIQVLPVLPVTERVFTDVGFVKEVVEW--- 650
              N++ GIL+    +   +F      E   GI +LP+ PV+  + ++  FV+E  EW   
Sbjct: 1011 IGNKVSGILFDNIIDYTTYFGTN--TEYIHGIHMLPITPVSSNIRSET-FVEE--EWQTK 1070

BLAST of Csa5G647400 vs. Swiss-Prot
Match: ENG1_SCHPO (Endo-1,3(4)-beta-glucanase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=eng1 PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 2.2e-27
Identity = 121/500 (24.20%), Postives = 216/500 (43.20%), Query Frame = 1

Query: 178 KHIIRLNNGRSWVLYS-SSAIYLVKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRY 237
           K+ + +++   W++Y    ++ L +S S  +V S  F G I++A +P     +E + D Y
Sbjct: 226 KYRVTMSDNHVWLIYIYGDSLTLTESTSQMLVGSNTFNGYIQIAKIPLGDGTAEALYDTY 285

Query: 238 SGCY----PVSGFVK-LLGGFGFEYKWQKKGSGGLL--MLAHTLHREILPRDQTVLQNIR 297
           +G Y     +SG+V+  +G + F++      S   L  +L H +   +     T +  + 
Sbjct: 286 AGVYITGISISGYVEDAVGYYSFDFTTAGDTSVEPLFFLLPHQVDTAVSGTKVTSI--VL 345

Query: 298 YSSIDGDLLGVVGDS--------WDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATL 357
            S + GD+    G+S         D+ F P   T   I   +     EI+A +       
Sbjct: 346 ASLVSGDMNAAAGNSITFAEAIPQDIGFLPWSPTGGQIGYSEEAL--EIIAEVAGTELGE 405

Query: 358 NATELSSMPASYFYGKLLARAARLALIAEEVNGGAGVIPAVVKFLKNGIQPWLIGK--FP 417
           + +  S++ + Y+ GK+LA+ A L +   ++ G        ++ L+     ++  +  +P
Sbjct: 406 DFSANSNLNSMYYSGKVLAKYAMLCVTINDILGDETSSEQCIQKLEAAFARFVDNQQIYP 465

Query: 418 KNGFLYERKWAGLVTKNGATSTT-EDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNW--GK 477
                Y+  W G+V+  G +  +  DFG   YNDHHFH GYFV++ AV+  +DP+W    
Sbjct: 466 ---LTYDNTWKGVVSVAGLSGDSLADFGNSYYNDHHFHYGYFVFTAAVIGHIDPDWINTG 525

Query: 478 QYKPQAYALLYDYMNFRPKKSQFSIP-FRNFDFWKLHSWAAGLTEFPDGRNQESTTEAVN 537
             K     L+ D  N  P  +    P  R  D +  H WA+GL E  DG+++EST+E  N
Sbjct: 526 NNKEWVNFLVRDVAN--PSSNDPYFPKHRMIDIYHGHGWASGLFESNDGKDEESTSEDYN 585

Query: 538 AYYAAALMGLAYDDESLTAAGSTLTAAEITATQTWWHVKRENNGIYDKGFTEENRMVGIL 597
            ++   L G    D  +    + +   E  A   +       +G       + N + GI 
Sbjct: 586 FFFGMKLWGQVIGDSDMEDRANIILGIERNALNKYMLYA---DGNVQPTSMQPNYVAGIT 645

Query: 598 WSAARESRLWFAPAEWRECRVGIQVLPVLPVTERVFTDVGFVKEVVEWVQPALEREDAGE 656
           +        +F      E   GI +LP+ P++  +      + E    +   ++  D+  
Sbjct: 646 FMNKITHTTYFGTN--IEYIQGIHMLPITPISAFIRGPSFVLAEWNALLASVIDYVDS-- 705

BLAST of Csa5G647400 vs. TrEMBL
Match: A0A0A0KW06_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G647400 PE=4 SV=1)

HSP 1 Score: 1387.9 bits (3591), Expect = 0.0e+00
Identity = 684/684 (100.00%), Postives = 684/684 (100.00%), Query Frame = 1

Query: 1   MEETSKPSPISTLFFLLLLTAAAVSAQFPFPETTSTAVPDPGKFFSPNLLSSPLPTNSFF 60
           MEETSKPSPISTLFFLLLLTAAAVSAQFPFPETTSTAVPDPGKFFSPNLLSSPLPTNSFF
Sbjct: 1   MEETSKPSPISTLFFLLLLTAAAVSAQFPFPETTSTAVPDPGKFFSPNLLSSPLPTNSFF 60

Query: 61  QNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRISNSSITQLQFFPDLVISSPKKTFNTT 120
           QNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRISNSSITQLQFFPDLVISSPKKTFNTT
Sbjct: 61  QNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRISNSSITQLQFFPDLVISSPKKTFNTT 120

Query: 121 HFISSFSDLGVDLDIGVFRFHLVRGSPYLTFSVLKTSSVLISTSNGVRSVDSYEDYTKHI 180
           HFISSFSDLGVDLDIGVFRFHLVRGSPYLTFSVLKTSSVLISTSNGVRSVDSYEDYTKHI
Sbjct: 121 HFISSFSDLGVDLDIGVFRFHLVRGSPYLTFSVLKTSSVLISTSNGVRSVDSYEDYTKHI 180

Query: 181 IRLNNGRSWVLYSSSAIYLVKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCY 240
           IRLNNGRSWVLYSSSAIYLVKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCY
Sbjct: 181 IRLNNGRSWVLYSSSAIYLVKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCY 240

Query: 241 PVSGFVKLLGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNIRYSSIDGDLLGV 300
           PVSGFVKLLGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNIRYSSIDGDLLGV
Sbjct: 241 PVSGFVKLLGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNIRYSSIDGDLLGV 300

Query: 301 VGDSWDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATLNATELSSMPASYFYGKLLA 360
           VGDSWDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATLNATELSSMPASYFYGKLLA
Sbjct: 301 VGDSWDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATLNATELSSMPASYFYGKLLA 360

Query: 361 RAARLALIAEEVNGGAGVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATS 420
           RAARLALIAEEVNGGAGVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATS
Sbjct: 361 RAARLALIAEEVNGGAGVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATS 420

Query: 421 TTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKKSQFS 480
           TTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKKSQFS
Sbjct: 421 TTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKKSQFS 480

Query: 481 IPFRNFDFWKLHSWAAGLTEFPDGRNQESTTEAVNAYYAAALMGLAYDDESLTAAGSTLT 540
           IPFRNFDFWKLHSWAAGLTEFPDGRNQESTTEAVNAYYAAALMGLAYDDESLTAAGSTLT
Sbjct: 481 IPFRNFDFWKLHSWAAGLTEFPDGRNQESTTEAVNAYYAAALMGLAYDDESLTAAGSTLT 540

Query: 541 AAEITATQTWWHVKRENNGIYDKGFTEENRMVGILWSAARESRLWFAPAEWRECRVGIQV 600
           AAEITATQTWWHVKRENNGIYDKGFTEENRMVGILWSAARESRLWFAPAEWRECRVGIQV
Sbjct: 541 AAEITATQTWWHVKRENNGIYDKGFTEENRMVGILWSAARESRLWFAPAEWRECRVGIQV 600

Query: 601 LPVLPVTERVFTDVGFVKEVVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKL 660
           LPVLPVTERVFTDVGFVKEVVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKL
Sbjct: 601 LPVLPVTERVFTDVGFVKEVVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKL 660

Query: 661 KKHDDGNSLSNLLWWIYSRPEGRR 685
           KKHDDGNSLSNLLWWIYSRPEGRR
Sbjct: 661 KKHDDGNSLSNLLWWIYSRPEGRR 684

BLAST of Csa5G647400 vs. TrEMBL
Match: A0A142BTQ9_CUCME (Beta-1,3-glucanase 2 OS=Cucumis melo GN=glu2 PE=2 SV=1)

HSP 1 Score: 1306.6 bits (3380), Expect = 0.0e+00
Identity = 643/683 (94.14%), Postives = 654/683 (95.75%), Query Frame = 1

Query: 1   MEETSKPSPISTLFFLLLLTAAAVSAQFPFPETTSTAVPDPGKFFSPNLLSSPLPTNSFF 60
           MEET K SP S LFF LLLTAA VSAQFPFPETTSTAVPDP KFFSPNLLSSPLPTNSFF
Sbjct: 1   MEETCKQSPFSILFFFLLLTAAPVSAQFPFPETTSTAVPDPSKFFSPNLLSSPLPTNSFF 60

Query: 61  QNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRISNSSITQLQFFPDLVISSPKKTFNTT 120
           QNFVLNNGD PEYIHPYLIRTANSSL VSYPSRISNSSI QLQFFPDLVISS  KT N T
Sbjct: 61  QNFVLNNGDSPEYIHPYLIRTANSSLFVSYPSRISNSSINQLQFFPDLVISSLNKTSNAT 120

Query: 121 HFISSFSDLGVDLDIGVFRFHLVRGSPYLTFSVLKTSSVLISTSNGVRSVDSYEDYTKHI 180
           HF+SSFSDLGVDLDIGVFR+HLVRGSPYLTFSVLKTS VLISTSNGVRSVDSYEDYTK+I
Sbjct: 121 HFVSSFSDLGVDLDIGVFRYHLVRGSPYLTFSVLKTSFVLISTSNGVRSVDSYEDYTKYI 180

Query: 181 IRLNNGRSWVLYSSSAIYLVKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCY 240
           IRLNNGRSWVLYSSSAIYL+KSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCY
Sbjct: 181 IRLNNGRSWVLYSSSAIYLIKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCY 240

Query: 241 PVSGFVKLLGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNIRYSSIDGDLLGV 300
           PVSGF KL GGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQN+RYSSIDGDLLGV
Sbjct: 241 PVSGFAKLSGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNLRYSSIDGDLLGV 300

Query: 301 VGDSWDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATLNATELSSMPASYFYGKLLA 360
           VGDSWDLKFNPIPITWHSINGI+SKFFPEIVAALKRDVATLN TELSS PASYFYGKLLA
Sbjct: 301 VGDSWDLKFNPIPITWHSINGINSKFFPEIVAALKRDVATLNVTELSSTPASYFYGKLLA 360

Query: 361 RAARLALIAEEVNGGAGVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATS 420
           RAARLALIAEEVN GA VIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATS
Sbjct: 361 RAARLALIAEEVNYGASVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATS 420

Query: 421 TTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKKSQFS 480
           T EDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNW KQYKPQAYALLYDYMNFRPKKSQFS
Sbjct: 421 TIEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWRKQYKPQAYALLYDYMNFRPKKSQFS 480

Query: 481 IPFRNFDFWKLHSWAAGLTEFPDGRNQESTTEAVNAYYAAALMGLAYDDESLTAAGSTLT 540
           IPFRNFDFWKLHSWAAGL EFPDGRNQEST+EAVNAYYAAALMGLAY D SLTAAGSTLT
Sbjct: 481 IPFRNFDFWKLHSWAAGLAEFPDGRNQESTSEAVNAYYAAALMGLAYGDASLTAAGSTLT 540

Query: 541 AAEITATQTWWHVKRENNGIYDKGFTEENRMVGILWSAARESRLWFAPAEWRECRVGIQV 600
           AAEITA+QTWWHVK+E NGIYDKGF EENRMVGILWSAARESRLWFAPAEWRECR+GIQV
Sbjct: 541 AAEITASQTWWHVKKEKNGIYDKGFAEENRMVGILWSAARESRLWFAPAEWRECRLGIQV 600

Query: 601 LPVLPVTERVFTDVGFVKEVVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKL 660
           LPVLPVTER+FTDVGFVKE VEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKL
Sbjct: 601 LPVLPVTERMFTDVGFVKEAVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKL 660

Query: 661 KKHDDGNSLSNLLWWIYSRPEGR 684
           KKHDDGNSLSNLLWWIYSRPEGR
Sbjct: 661 KKHDDGNSLSNLLWWIYSRPEGR 683

BLAST of Csa5G647400 vs. TrEMBL
Match: W9R5F5_9ROSA (Endo-1,3(4)-beta-glucanase 1 OS=Morus notabilis GN=L484_009128 PE=4 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 8.6e-225
Identity = 393/665 (59.10%), Postives = 490/665 (73.68%), Query Frame = 1

Query: 25  SAQFPFPETTSTAVPDPGKFFSPNLLSSPLPTNSFFQNFVLNNGDLPEYIHPYLIRTANS 84
           +A F FP+  ST V DP  FFSP+LLS+PLPTNSFFQNFVL NGD PEYIHPYL++++NS
Sbjct: 65  NAPFLFPKAQSTVVSDPSLFFSPDLLSTPLPTNSFFQNFVLKNGDQPEYIHPYLVKSSNS 124

Query: 85  SLSVSYPSRISNSSITQLQFFPDLVISSPKKTFNTT---HFISSFSDLGVDLDI--GVFR 144
           SLSV YPSR+SNS+     F  DL +S+ +KT   +   H ISSF+DL + LDI     R
Sbjct: 125 SLSVCYPSRLSNSAFLYQIFNADLTVSAAEKTAPDSQKPHLISSFNDLSLTLDIPSSNLR 184

Query: 145 FHLVRGSPYLTFSVLKTSSVLISTSNGVRSVDSYEDYTKHIIRLNNGRSWVLYSSSAIYL 204
           F LVRGSP+LT +    + + IST + + S  S    TK+ ++LNN ++W++Y+SS I  
Sbjct: 185 FFLVRGSPFLTCTASAKTEISISTIHAILSFSSNNSQTKYTVKLNNNQTWLIYTSSPISF 244

Query: 205 VKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCYPVSGFVKLLGGFGFEYKWQ 264
             S S  ++TS GF G++R+A+LPDS  E E ILDR+S CYPVSG V     F  EYKW+
Sbjct: 245 --SHSLSLLTSNGFSGILRIAILPDSNPEFEAILDRFSSCYPVSGEVSFTKPFCLEYKWE 304

Query: 265 KKGSGGLLMLAHTLHREILPRDQ---TVLQNIRYSSIDGDLLGVVGDSWDLKFNPIPITW 324
           KKG G LLMLAH LH ++L  D    TVL+  +Y SIDGDL+GVVGDSW LK +P+ +TW
Sbjct: 305 KKGWGDLLMLAHPLHLKLLKGDDRGVTVLEEFKYKSIDGDLVGVVGDSWTLKPDPVSVTW 364

Query: 325 HSINGIDSKFFPEIVAALKRDVATLNATELSSMPASYFYGKLLARAARLALIAEEVNGGA 384
           HSI G+  + + EIVAAL++DV  L+A  +++  ASYFYGKL+ARAAR ALIAEEV    
Sbjct: 365 HSIEGVKEEAYEEIVAALQKDVEALDAAAITTN-ASYFYGKLIARAARFALIAEEVRF-F 424

Query: 385 GVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATSTTEDFGFGIYNDHHFH 444
            VIP + KFLK  I+PWL G F  NGFLYE KW G++TK G      DFGFGIYNDHHFH
Sbjct: 425 DVIPKIRKFLKEAIEPWLDGTFNGNGFLYEEKWGGIITKQGQGDGGADFGFGIYNDHHFH 484

Query: 445 LGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKKSQFSIPFRNFDFWKLHSWAA 504
           LG+F+Y+IAVLAK+DP WG++YKPQAY+++ D+MN   +        R FD +KLHSWA 
Sbjct: 485 LGHFLYAIAVLAKIDPAWGRKYKPQAYSMMADFMNLSRRAKSNYPRLRCFDLYKLHSWAG 544

Query: 505 GLTEFPDGRNQESTTEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEITATQTWWHVKRE 564
           GLTEF DGRNQES++EAVNAYY+AALMGLAY D  L A GS LTA EI A Q WWHVK E
Sbjct: 545 GLTEFADGRNQESSSEAVNAYYSAALMGLAYGDTHLVAIGSLLTALEIQAAQMWWHVK-E 604

Query: 565 NNGIYDKGFTEENRMVGILWSAARESRLWFAPAEWRECRVGIQVLPVLPVTERVFTDVGF 624
            + +YD+ FT ENR+VG+LWS  R+S LWFAPA WRECR+GIQVLP+LP+TE +F+DVGF
Sbjct: 605 GDKLYDEDFTRENRVVGVLWSNKRDSGLWFAPAAWRECRLGIQVLPLLPITEVLFSDVGF 664

Query: 625 VKEVVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHDDGNSLSNLLWWI 682
            +E+V W  PAL+RE  GEGWKGF YALEGIYDK++A++K+K L   DDGNSL+NLLWWI
Sbjct: 665 TRELVNWTLPALQREGVGEGWKGFVYALEGIYDKETALQKIKSLNGFDDGNSLTNLLWWI 724

BLAST of Csa5G647400 vs. TrEMBL
Match: A0A061EHI7_THECC (Glycosyl hydrolase family 81 protein OS=Theobroma cacao GN=TCM_019610 PE=4 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 7.3e-224
Identity = 390/660 (59.09%), Postives = 489/660 (74.09%), Query Frame = 1

Query: 28  FPFPETTSTAVPDPGKFFSPNLLSSPLPTNSFFQNFVLNNGDLPEYIHPYLIRTANSSLS 87
           F FP   S+ +PDP  FFSP LLS+PLPTNSFFQNF L NGD PEYIHPYLI++A+SSLS
Sbjct: 51  FLFPGVQSSVLPDPSVFFSPKLLSTPLPTNSFFQNFTLKNGDQPEYIHPYLIKSADSSLS 110

Query: 88  VSYPSRISNSSITQLQFFPDLVISSPKKTFNTT---HFISSFSDLGVDLDIGV--FRFHL 147
           +SYPS   NSS     F  DL I +  +T  T+   H ISS+SDL + LD      RF+L
Sbjct: 111 ISYPSHFHNSSFLYQIFISDLTIFATDRTNPTSQKNHIISSYSDLSLTLDFPCSNLRFYL 170

Query: 148 VRGSPYLTFSVLKTSSVLISTSNGVRSVDSYEDYTKHIIRLNNGRSWVLYSSSAIYLVKS 207
           VRGSP+LT  V   + + IST + + S  S    TKH I+LNN ++W++Y+SSAI L  S
Sbjct: 171 VRGSPFLTCFVTGNTPISISTIHAILSFSSNTSLTKHTIKLNNNQTWLIYTSSAISLNHS 230

Query: 208 KSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCYPVSGFVKLLGGFGFEYKWQKKG 267
            S  ++TSGGF G+IR+AVLPDS    E ILD+YS CYP+SG   L   F  EYKW+KKG
Sbjct: 231 LS--LITSGGFSGIIRIAVLPDSDPTYESILDQYSSCYPISGDAVLTKPFCLEYKWEKKG 290

Query: 268 SGGLLMLAHTLHREILPRDQ---TVLQNIRYSSIDGDLLGVVGDSWDLKFNPIPITWHSI 327
            G LLMLAH LH  IL RD    TVL+N +Y SIDG+L+G+VGDSW LK + + +TWHSI
Sbjct: 291 WGDLLMLAHPLHLNILSRDDSEVTVLENFKYKSIDGELVGIVGDSWVLKPHQVSVTWHSI 350

Query: 328 NGIDSKFFPEIVAALKRDVATLNATELSSMPASYFYGKLLARAARLALIAEEVNGGAGVI 387
            G+  + + EIV AL +DV  L+++ +++  +SYFYGKL+ARAARLALIAEEV     VI
Sbjct: 351 KGVKEESYHEIVTALSKDVEALDSSAITTT-SSYFYGKLIARAARLALIAEEVCF-LDVI 410

Query: 388 PAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATSTTEDFGFGIYNDHHFHLGY 447
           PA+ KFLK+ I+PWL G F  NGFLY+ KW G+VTK G+T +  DFGFGIYNDHH+H+GY
Sbjct: 411 PAIRKFLKDTIEPWLDGTFNGNGFLYDSKWGGIVTKQGSTDSGADFGFGIYNDHHYHIGY 470

Query: 448 FVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKKSQFSIPFRNFDFWKLHSWAAGLT 507
           F+Y+IAVLAK+DP WG++YKPQAY L+ D+MN   + +      R FD +KLHSWA GLT
Sbjct: 471 FLYAIAVLAKIDPAWGRRYKPQAYTLVADFMNLGRRSNSNYPRLRCFDLYKLHSWAGGLT 530

Query: 508 EFPDGRNQESTTEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEITATQTWWHVKRENNG 567
           EF DGRNQEST+EAVNAYY+AALMGLAY D  L A GSTL A EI A QTWWHVK E + 
Sbjct: 531 EFADGRNQESTSEAVNAYYSAALMGLAYGDTHLVATGSTLAAMEIQAAQTWWHVK-EGDN 590

Query: 568 IYDKGFTEENRMVGILWSAARESRLWFAPAEWRECRVGIQVLPVLPVTERVFTDVGFVKE 627
           +Y++ FT ENR+VG+LW+  R+S LWFAPA+WRECR+GIQ+LP+LP++E +F+D+ F +E
Sbjct: 591 LYEEEFTTENRVVGVLWANKRDSGLWFAPADWRECRLGIQLLPILPISEVLFSDISFTRE 650

Query: 628 VVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHDDGNSLSNLLWWIYSR 680
           +V W  PAL RE  GEGWKGF YALE IYDK+SA+EK++ L  +DDGNSL+NLLWW++SR
Sbjct: 651 LVNWTLPALGREGVGEGWKGFVYALEAIYDKESALEKIRNLNGYDDGNSLTNLLWWVHSR 705

BLAST of Csa5G647400 vs. TrEMBL
Match: A0A0D2PNP6_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_001G082200 PE=4 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 1.6e-223
Identity = 389/660 (58.94%), Postives = 490/660 (74.24%), Query Frame = 1

Query: 28  FPFPETTSTAVPDPGKFFSPNLLSSPLPTNSFFQNFVLNNGDLPEYIHPYLIRTANSSLS 87
           F FP+  S+ + DP +FFSPNLLSSPLPTNSFFQNF L NGD PEYIHPYLI++A+SSLS
Sbjct: 54  FLFPKVQSSVLSDPSRFFSPNLLSSPLPTNSFFQNFTLKNGDQPEYIHPYLIKSADSSLS 113

Query: 88  VSYPSRISNSSITQLQFFPDLVISSPKKTFNTT---HFISSFSDLGVDLDI--GVFRFHL 147
           +SYPS   N S     F PDL I +  KT +T+   H ISS+SDL + LDI     RF L
Sbjct: 114 ISYPSFFHNPSFIYQIFVPDLTIFATDKTTSTSDKSHIISSYSDLSLTLDIPSSNLRFFL 173

Query: 148 VRGSPYLTFSVLKTSSVLISTSNGVRSVDSYEDYTKHIIRLNNGRSWVLYSSSAIYLVKS 207
           VRGSP+LT SV   + + IST + + S  S    TKH I+LNNG++W++Y+SS+I L  S
Sbjct: 174 VRGSPFLTCSVTGNTPISISTVHAILSFSSNSSLTKHTIKLNNGQTWIIYASSSISLNHS 233

Query: 208 KSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCYPVSGFVKLLGGFGFEYKWQKKG 267
            S  ++TSGGF G++R+AVL DS    E ILD++  CYP SG   L   F  EYKW+KKG
Sbjct: 234 VS--LITSGGFSGILRIAVLSDSDPTCESILDQFCSCYPTSGDAVLTKPFCVEYKWEKKG 293

Query: 268 SGGLLMLAHTLHREILPRDQT---VLQNIRYSSIDGDLLGVVGDSWDLKFNPIPITWHSI 327
            G LLMLAH LH +IL +D     VL+N RY SIDGDL+G+VGDSW LK + + +TWHSI
Sbjct: 294 WGDLLMLAHPLHLDILCKDYCDVKVLENFRYKSIDGDLVGIVGDSWVLKPHQVSVTWHSI 353

Query: 328 NGIDSKFFPEIVAALKRDVATLNATELSSMPASYFYGKLLARAARLALIAEEVNGGAGVI 387
            G++ + + EIV AL +DV  L+++ +++  +SYFYGKL+ARAARLALIAEEV     VI
Sbjct: 354 KGVNEESYAEIVTALAKDVDALDSSAITTK-SSYFYGKLIARAARLALIAEEVCF-LDVI 413

Query: 388 PAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATSTTEDFGFGIYNDHHFHLGY 447
           PA+ KFLK+ I+PWL G F  NGFLYE KW G+VTK G+T +  DFGFG+YNDHH+H+GY
Sbjct: 414 PAIRKFLKDTIEPWLDGTFNGNGFLYESKWGGIVTKQGSTDSGADFGFGVYNDHHYHIGY 473

Query: 448 FVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKKSQFSIPFRNFDFWKLHSWAAGLT 507
           F+Y IAVL K+DP WG++YKPQAY+L+ D+MN   + +      R FDF+KLHSWA GLT
Sbjct: 474 FLYGIAVLTKIDPAWGRRYKPQAYSLVADFMNLGRRSNSNYPRVRCFDFYKLHSWAGGLT 533

Query: 508 EFPDGRNQESTTEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEITATQTWWHVKRENNG 567
           EF DGRNQEST+EAVNAYY+AALMGLAY D  L A GSTLTA EI A QTWWHVK E + 
Sbjct: 534 EFADGRNQESTSEAVNAYYSAALMGLAYGDTHLVATGSTLTALEIQAAQTWWHVK-EGDN 593

Query: 568 IYDKGFTEENRMVGILWSAARESRLWFAPAEWRECRVGIQVLPVLPVTERVFTDVGFVKE 627
           +Y + FT ENR+VG+LW+  R+S LWFAP +WRECR+GIQ+LP+LP++E +F+ + F ++
Sbjct: 594 LYGEEFTRENRVVGVLWANKRDSGLWFAPPDWRECRLGIQLLPILPISEVLFSAIEFTRQ 653

Query: 628 VVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHDDGNSLSNLLWWIYSR 680
           +V W  PAL RE  GEGWKGF YALE IYDK+SA+EK++ L  HDDGNSL+NLLWW++SR
Sbjct: 654 LVNWTLPALGREGVGEGWKGFVYALEAIYDKESALEKIRNLSGHDDGNSLTNLLWWVHSR 708

BLAST of Csa5G647400 vs. TAIR10
Match: AT5G15870.1 (AT5G15870.1 glycosyl hydrolase family 81 protein)

HSP 1 Score: 728.4 bits (1879), Expect = 4.1e-210
Identity = 373/664 (56.17%), Postives = 469/664 (70.63%), Query Frame = 1

Query: 28  FPFPETTSTAVPDPGKFFSPNLLSSPLPTNSFFQNFVLNNGDLPEYIHPYLIRTANSSLS 87
           F FP + S+ +PDP +FFS +LLSSPLPTNSFFQNF LNNGD  EY HPY+I+ + SSLS
Sbjct: 55  FLFPRSDSSVLPDPSRFFSHDLLSSPLPTNSFFQNFTLNNGDQAEYFHPYIIKPSTSSLS 114

Query: 88  VSYPSRISNSSITQLQFFPDLVISS---PKKTFNTTHFISSFSDLGVDLDI--GVFRFHL 147
           +SYPS   NS+     F  D+ I+    P      +H ISSFSDLGV LD      RF L
Sbjct: 115 ISYPSLSHNSAFIYEAFNADITITGSDGPDPHSRKSHLISSFSDLGVTLDFPSSNLRFFL 174

Query: 148 VRGSPYLTFSVLKTSSVLISTSNGVRSVDSYEDYTKHIIRLNNGRSWVLYSSSAIYLVKS 207
           VRGSP++TFSV   SS+ IST + V S+      TK+ ++LNN ++W++Y+SS I L K 
Sbjct: 175 VRGSPFITFSV--NSSITISTIHAVLSLSGNTSSTKYTVKLNNNQTWLIYASSPINLTKD 234

Query: 208 KSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCYPVSGFVKLLGGFGFEYKWQKKG 267
             + I    GF G+IR+ VLP+     E ILD +S  YPVSG       F  EYKW+K+G
Sbjct: 235 GVSSINCGDGFSGIIRIVVLPNPNPYFETILDGFSCSYPVSGDADFTKPFALEYKWEKRG 294

Query: 268 SGGLLMLAHTLHREILPRDQ---TVLQNIRYSSIDGDLLGVVGDSWDLKFNPIPITWHSI 327
            G LLMLAH LH ++L  +    TVL N +Y+SIDGDL+GV+GDSW LK +P+ +TWHSI
Sbjct: 295 YGDLLMLAHPLHLKLLSTNDCSITVLDNFKYNSIDGDLVGVIGDSWVLKPDPVSVTWHSI 354

Query: 328 NGIDSKFFPEIVAALKRDVATLNAT-ELSSMPASYFYGKLLARAARLALIAEEVNGGAGV 387
            G+      EI++AL +DV  L+++ E+++  +SYFY KL+ARAARLALIAEEV     V
Sbjct: 355 KGVQEDSHQEIISALIKDVNALDSSAEVTN--SSYFYAKLIARAARLALIAEEVCY-LDV 414

Query: 388 IPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATSTTEDFGFGIYNDHHFHLG 447
           IP +  +LKN I+PWL G F  NGFLY+ KW G++TK G+  +  DFGFGIYNDHH+HLG
Sbjct: 415 IPKIRTYLKNMIEPWLNGSFGPNGFLYDPKWGGVITKLGSRDSGADFGFGIYNDHHYHLG 474

Query: 448 YFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPK--KSQFSIP-FRNFDFWKLHSWA 507
           YFVY+IAVLAK+DP WGK+Y+PQAY L+ DY+    K  KS  + P  R FD +KLHSWA
Sbjct: 475 YFVYAIAVLAKIDPLWGKRYRPQAYTLMADYLTLGKKGAKSNSNYPRLRCFDLFKLHSWA 534

Query: 508 AGLTEFPDGRNQESTTEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEITATQTWWHVKR 567
            GLTEF DGRNQEST+EAVNAYY+AAL+GLAY D  L AA S +   EI A + WW VK 
Sbjct: 535 GGLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLVAAASMVLTLEIHAAKMWWQVK- 594

Query: 568 ENNGIYDKGFTEENRMVGILWSAARESRLWFAPAEWRECRVGIQVLPVLPVTERVFTDVG 627
           E++ IY + FT ENR+VG+LWS  R+S LWFAP EW+ECR+GIQ+LP+LPV+E +F+DV 
Sbjct: 595 EDDAIYPQDFTSENRVVGVLWSTKRDSGLWFAPKEWKECRLGIQLLPLLPVSEVLFSDVT 654

Query: 628 FVKEVVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHDDGNSLSNLLWW 680
           FVK++V W  PAL R+  GEGWKGF YALE +YDK  A+EK+K L   DDGNSLSNLLWW
Sbjct: 655 FVKQLVNWTMPALARDSVGEGWKGFVYALESMYDKDGAMEKIKGLNGFDDGNSLSNLLWW 712

BLAST of Csa5G647400 vs. TAIR10
Match: AT1G18310.1 (AT1G18310.1 glycosyl hydrolase family 81 protein)

HSP 1 Score: 677.2 bits (1746), Expect = 1.1e-194
Identity = 359/691 (51.95%), Postives = 446/691 (64.54%), Query Frame = 1

Query: 22  AAVSAQFPFPETTSTAVPDPGKFFSPNLLSSPLPTNSFFQNFVLNNGDLPEYIHPYLIRT 81
           A+ +A F FPET S+ +PDP +FFSP+LLS+PLPTNSFFQNF L NGD  EY HPYLI++
Sbjct: 2   ASSNALFLFPETKSSVLPDPSRFFSPDLLSNPLPTNSFFQNFTLKNGDQAEYFHPYLIKS 61

Query: 82  ANSSLSVSYPSRISNSSITQLQFFPDLVISS---PKKTFNTTHFISSFSDLGVDLDI--G 141
            +SSL +SYPS   NS      F  D++IS    P      TH ISSFSDL V LD    
Sbjct: 62  VDSSLCISYPSLFHNSDFFYEVFTADIIISGSNGPGPRSRKTHIISSFSDLVVTLDFPSS 121

Query: 142 VFRFHLVRGSPYLTFSVLKTSSVLISTSNGVRSVDSYEDYTKHIIRLNNGRSWVLYSSSA 201
             RF LVRGSP        +SS                  TK   +L N + W++Y+SS 
Sbjct: 122 NLRFFLVRGSP--------SSS------------------TKWSDKLTNNQRWLIYASSP 181

Query: 202 IYLVKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCYPVSGFVKLLGGFGFEY 261
           I L K   + I   GGF G++R+ VLP S    E  LDR+S CYPVSG       F  +Y
Sbjct: 182 IDLTKHGDSSIHCRGGFTGIVRITVLPGSNPGYESTLDRFSSCYPVSGDADFTKPFTLKY 241

Query: 262 KWQKKGSGGLLMLAHTLHREILPRDQ-----------------------TVLQNIRYSSI 321
            W+K+GSG LLMLAH LH ++L +D                        TVL + RY SI
Sbjct: 242 MWEKRGSGDLLMLAHPLHLKLLAKDASSLFQQSTLLLVCPDRGCTNSSVTVLDHFRYKSI 301

Query: 322 DGDLLGVVGDSWDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATLNAT-ELSSMPAS 381
           DGDL+GVVGDSW LK + + +TWHS+ G+    + EI++AL +DV  LN++ E++S  +S
Sbjct: 302 DGDLVGVVGDSWVLKPDSVSVTWHSMKGVKKDSYKEIISALGKDVNGLNSSAEVTS--SS 361

Query: 382 YFYGKLLARAARLALIAEEVNGGAGVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGL 441
           YFYGKL+ARAAR ALIAEEV     VIP +V +LKN I+PWL G F  NGFLY+ KW GL
Sbjct: 362 YFYGKLIARAARFALIAEEVCY-LDVIPKIVTYLKNMIEPWLDGSFKPNGFLYDPKWGGL 421

Query: 442 VTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNF 501
           +TK G+  +  DFGFGIYNDHH+H+GYF+Y+IAVLAK DP WG++Y+ QAY+LL D+M F
Sbjct: 422 ITKQGSKDSQADFGFGIYNDHHYHIGYFLYAIAVLAKFDPLWGERYRAQAYSLLADFMTF 481

Query: 502 RPK---KSQFSIP-FRNFDFWKLHSWAAGLTEFPDGRNQESTTEAVNAYYAAALMGLAYD 561
             K    S  S P  RNFD +KLHSWA GLTEF DGRNQEST+EAVNAYY+AAL+GLAY 
Sbjct: 482 GRKDDNNSNSSYPRLRNFDLFKLHSWAGGLTEFWDGRNQESTSEAVNAYYSAALLGLAYG 541

Query: 562 DESLTAAGSTLTAAEITATQTWWHVKRENNGIYDKGFTEENRMVGILWSAARESRLWFAP 621
           D+ L    ST+   EI A + WW VK+    +Y K FT ENR+VG+LWS  R+S LWF P
Sbjct: 542 DKHLVETASTIMTLEIHAAKMWWQVKK-GEALYPKDFTAENRVVGVLWSTKRDSSLWFGP 601

Query: 622 AEWRECRVGIQVLPVLPVTERVFTDVGFVKEVVEWVQPALEREDAGEGWKGFAYALEGIY 680
            EW+ECR+GIQ+LP+LP              +V W  PAL+R   GEGWKGF YALE +Y
Sbjct: 602 KEWKECRLGIQLLPILP--------------LVNWTLPALQRNGVGEGWKGFLYALESLY 648

BLAST of Csa5G647400 vs. NCBI nr
Match: gi|778707755|ref|XP_011656051.1| (PREDICTED: putative endo-1,3(4)-beta-glucanase 2 [Cucumis sativus])

HSP 1 Score: 1387.9 bits (3591), Expect = 0.0e+00
Identity = 684/684 (100.00%), Postives = 684/684 (100.00%), Query Frame = 1

Query: 1   MEETSKPSPISTLFFLLLLTAAAVSAQFPFPETTSTAVPDPGKFFSPNLLSSPLPTNSFF 60
           MEETSKPSPISTLFFLLLLTAAAVSAQFPFPETTSTAVPDPGKFFSPNLLSSPLPTNSFF
Sbjct: 1   MEETSKPSPISTLFFLLLLTAAAVSAQFPFPETTSTAVPDPGKFFSPNLLSSPLPTNSFF 60

Query: 61  QNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRISNSSITQLQFFPDLVISSPKKTFNTT 120
           QNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRISNSSITQLQFFPDLVISSPKKTFNTT
Sbjct: 61  QNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRISNSSITQLQFFPDLVISSPKKTFNTT 120

Query: 121 HFISSFSDLGVDLDIGVFRFHLVRGSPYLTFSVLKTSSVLISTSNGVRSVDSYEDYTKHI 180
           HFISSFSDLGVDLDIGVFRFHLVRGSPYLTFSVLKTSSVLISTSNGVRSVDSYEDYTKHI
Sbjct: 121 HFISSFSDLGVDLDIGVFRFHLVRGSPYLTFSVLKTSSVLISTSNGVRSVDSYEDYTKHI 180

Query: 181 IRLNNGRSWVLYSSSAIYLVKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCY 240
           IRLNNGRSWVLYSSSAIYLVKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCY
Sbjct: 181 IRLNNGRSWVLYSSSAIYLVKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCY 240

Query: 241 PVSGFVKLLGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNIRYSSIDGDLLGV 300
           PVSGFVKLLGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNIRYSSIDGDLLGV
Sbjct: 241 PVSGFVKLLGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNIRYSSIDGDLLGV 300

Query: 301 VGDSWDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATLNATELSSMPASYFYGKLLA 360
           VGDSWDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATLNATELSSMPASYFYGKLLA
Sbjct: 301 VGDSWDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATLNATELSSMPASYFYGKLLA 360

Query: 361 RAARLALIAEEVNGGAGVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATS 420
           RAARLALIAEEVNGGAGVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATS
Sbjct: 361 RAARLALIAEEVNGGAGVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATS 420

Query: 421 TTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKKSQFS 480
           TTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKKSQFS
Sbjct: 421 TTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKKSQFS 480

Query: 481 IPFRNFDFWKLHSWAAGLTEFPDGRNQESTTEAVNAYYAAALMGLAYDDESLTAAGSTLT 540
           IPFRNFDFWKLHSWAAGLTEFPDGRNQESTTEAVNAYYAAALMGLAYDDESLTAAGSTLT
Sbjct: 481 IPFRNFDFWKLHSWAAGLTEFPDGRNQESTTEAVNAYYAAALMGLAYDDESLTAAGSTLT 540

Query: 541 AAEITATQTWWHVKRENNGIYDKGFTEENRMVGILWSAARESRLWFAPAEWRECRVGIQV 600
           AAEITATQTWWHVKRENNGIYDKGFTEENRMVGILWSAARESRLWFAPAEWRECRVGIQV
Sbjct: 541 AAEITATQTWWHVKRENNGIYDKGFTEENRMVGILWSAARESRLWFAPAEWRECRVGIQV 600

Query: 601 LPVLPVTERVFTDVGFVKEVVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKL 660
           LPVLPVTERVFTDVGFVKEVVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKL
Sbjct: 601 LPVLPVTERVFTDVGFVKEVVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKL 660

Query: 661 KKHDDGNSLSNLLWWIYSRPEGRR 685
           KKHDDGNSLSNLLWWIYSRPEGRR
Sbjct: 661 KKHDDGNSLSNLLWWIYSRPEGRR 684

BLAST of Csa5G647400 vs. NCBI nr
Match: gi|1004356876|gb|AMP41497.1| (beta-1,3-glucanase 2 [Cucumis melo])

HSP 1 Score: 1306.6 bits (3380), Expect = 0.0e+00
Identity = 643/683 (94.14%), Postives = 654/683 (95.75%), Query Frame = 1

Query: 1   MEETSKPSPISTLFFLLLLTAAAVSAQFPFPETTSTAVPDPGKFFSPNLLSSPLPTNSFF 60
           MEET K SP S LFF LLLTAA VSAQFPFPETTSTAVPDP KFFSPNLLSSPLPTNSFF
Sbjct: 1   MEETCKQSPFSILFFFLLLTAAPVSAQFPFPETTSTAVPDPSKFFSPNLLSSPLPTNSFF 60

Query: 61  QNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRISNSSITQLQFFPDLVISSPKKTFNTT 120
           QNFVLNNGD PEYIHPYLIRTANSSL VSYPSRISNSSI QLQFFPDLVISS  KT N T
Sbjct: 61  QNFVLNNGDSPEYIHPYLIRTANSSLFVSYPSRISNSSINQLQFFPDLVISSLNKTSNAT 120

Query: 121 HFISSFSDLGVDLDIGVFRFHLVRGSPYLTFSVLKTSSVLISTSNGVRSVDSYEDYTKHI 180
           HF+SSFSDLGVDLDIGVFR+HLVRGSPYLTFSVLKTS VLISTSNGVRSVDSYEDYTK+I
Sbjct: 121 HFVSSFSDLGVDLDIGVFRYHLVRGSPYLTFSVLKTSFVLISTSNGVRSVDSYEDYTKYI 180

Query: 181 IRLNNGRSWVLYSSSAIYLVKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCY 240
           IRLNNGRSWVLYSSSAIYL+KSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCY
Sbjct: 181 IRLNNGRSWVLYSSSAIYLIKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCY 240

Query: 241 PVSGFVKLLGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNIRYSSIDGDLLGV 300
           PVSGF KL GGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQN+RYSSIDGDLLGV
Sbjct: 241 PVSGFAKLSGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNLRYSSIDGDLLGV 300

Query: 301 VGDSWDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATLNATELSSMPASYFYGKLLA 360
           VGDSWDLKFNPIPITWHSINGI+SKFFPEIVAALKRDVATLN TELSS PASYFYGKLLA
Sbjct: 301 VGDSWDLKFNPIPITWHSINGINSKFFPEIVAALKRDVATLNVTELSSTPASYFYGKLLA 360

Query: 361 RAARLALIAEEVNGGAGVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATS 420
           RAARLALIAEEVN GA VIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATS
Sbjct: 361 RAARLALIAEEVNYGASVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATS 420

Query: 421 TTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKKSQFS 480
           T EDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNW KQYKPQAYALLYDYMNFRPKKSQFS
Sbjct: 421 TIEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWRKQYKPQAYALLYDYMNFRPKKSQFS 480

Query: 481 IPFRNFDFWKLHSWAAGLTEFPDGRNQESTTEAVNAYYAAALMGLAYDDESLTAAGSTLT 540
           IPFRNFDFWKLHSWAAGL EFPDGRNQEST+EAVNAYYAAALMGLAY D SLTAAGSTLT
Sbjct: 481 IPFRNFDFWKLHSWAAGLAEFPDGRNQESTSEAVNAYYAAALMGLAYGDASLTAAGSTLT 540

Query: 541 AAEITATQTWWHVKRENNGIYDKGFTEENRMVGILWSAARESRLWFAPAEWRECRVGIQV 600
           AAEITA+QTWWHVK+E NGIYDKGF EENRMVGILWSAARESRLWFAPAEWRECR+GIQV
Sbjct: 541 AAEITASQTWWHVKKEKNGIYDKGFAEENRMVGILWSAARESRLWFAPAEWRECRLGIQV 600

Query: 601 LPVLPVTERVFTDVGFVKEVVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKL 660
           LPVLPVTER+FTDVGFVKE VEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKL
Sbjct: 601 LPVLPVTERMFTDVGFVKEAVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKL 660

Query: 661 KKHDDGNSLSNLLWWIYSRPEGR 684
           KKHDDGNSLSNLLWWIYSRPEGR
Sbjct: 661 KKHDDGNSLSNLLWWIYSRPEGR 683

BLAST of Csa5G647400 vs. NCBI nr
Match: gi|659119185|ref|XP_008459519.1| (PREDICTED: putative endo-1,3(4)-beta-glucanase 2 [Cucumis melo])

HSP 1 Score: 1306.6 bits (3380), Expect = 0.0e+00
Identity = 643/683 (94.14%), Postives = 654/683 (95.75%), Query Frame = 1

Query: 1   MEETSKPSPISTLFFLLLLTAAAVSAQFPFPETTSTAVPDPGKFFSPNLLSSPLPTNSFF 60
           MEET K SP S LFF LLLTAA VSAQFPFPETTSTAVPDP KFFSPNLLSSPLPTNSFF
Sbjct: 18  MEETCKQSPFSILFFFLLLTAAPVSAQFPFPETTSTAVPDPSKFFSPNLLSSPLPTNSFF 77

Query: 61  QNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRISNSSITQLQFFPDLVISSPKKTFNTT 120
           QNFVLNNGD PEYIHPYLIRTANSSL VSYPSRISNSSI QLQFFPDLVISS  KT N T
Sbjct: 78  QNFVLNNGDSPEYIHPYLIRTANSSLFVSYPSRISNSSINQLQFFPDLVISSLNKTSNAT 137

Query: 121 HFISSFSDLGVDLDIGVFRFHLVRGSPYLTFSVLKTSSVLISTSNGVRSVDSYEDYTKHI 180
           HF+SSFSDLGVDLDIGVFR+HLVRGSPYLTFSVLKTS VLISTSNGVRSVDSYEDYTK+I
Sbjct: 138 HFVSSFSDLGVDLDIGVFRYHLVRGSPYLTFSVLKTSFVLISTSNGVRSVDSYEDYTKYI 197

Query: 181 IRLNNGRSWVLYSSSAIYLVKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCY 240
           IRLNNGRSWVLYSSSAIYL+KSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCY
Sbjct: 198 IRLNNGRSWVLYSSSAIYLIKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCY 257

Query: 241 PVSGFVKLLGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNIRYSSIDGDLLGV 300
           PVSGF KL GGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQN+RYSSIDGDLLGV
Sbjct: 258 PVSGFAKLSGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNLRYSSIDGDLLGV 317

Query: 301 VGDSWDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATLNATELSSMPASYFYGKLLA 360
           VGDSWDLKFNPIPITWHSINGI+SKFFPEIVAALKRDVATLN TELSS PASYFYGKLLA
Sbjct: 318 VGDSWDLKFNPIPITWHSINGINSKFFPEIVAALKRDVATLNVTELSSTPASYFYGKLLA 377

Query: 361 RAARLALIAEEVNGGAGVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATS 420
           RAARLALIAEEVN GA VIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATS
Sbjct: 378 RAARLALIAEEVNYGASVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATS 437

Query: 421 TTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKKSQFS 480
           T EDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNW KQYKPQAYALLYDYMNFRPKKSQFS
Sbjct: 438 TIEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWRKQYKPQAYALLYDYMNFRPKKSQFS 497

Query: 481 IPFRNFDFWKLHSWAAGLTEFPDGRNQESTTEAVNAYYAAALMGLAYDDESLTAAGSTLT 540
           IPFRNFDFWKLHSWAAGL EFPDGRNQEST+EAVNAYYAAALMGLAY D SLTAAGSTLT
Sbjct: 498 IPFRNFDFWKLHSWAAGLAEFPDGRNQESTSEAVNAYYAAALMGLAYGDASLTAAGSTLT 557

Query: 541 AAEITATQTWWHVKRENNGIYDKGFTEENRMVGILWSAARESRLWFAPAEWRECRVGIQV 600
           AAEITA+QTWWHVK+E NGIYDKGF EENRMVGILWSAARESRLWFAPAEWRECR+GIQV
Sbjct: 558 AAEITASQTWWHVKKEKNGIYDKGFAEENRMVGILWSAARESRLWFAPAEWRECRLGIQV 617

Query: 601 LPVLPVTERVFTDVGFVKEVVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKL 660
           LPVLPVTER+FTDVGFVKE VEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKL
Sbjct: 618 LPVLPVTERMFTDVGFVKEAVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKL 677

Query: 661 KKHDDGNSLSNLLWWIYSRPEGR 684
           KKHDDGNSLSNLLWWIYSRPEGR
Sbjct: 678 KKHDDGNSLSNLLWWIYSRPEGR 700

BLAST of Csa5G647400 vs. NCBI nr
Match: gi|720029881|ref|XP_010265349.1| (PREDICTED: putative endo-1,3(4)-beta-glucanase 2 [Nelumbo nucifera])

HSP 1 Score: 792.7 bits (2046), Expect = 5.0e-226
Identity = 391/662 (59.06%), Postives = 498/662 (75.23%), Query Frame = 1

Query: 28  FPFPETTSTAVPDPGKFFSPNLLSSPLPTNSFFQNFVLNNGDLPEYIHPYLIRTANSSLS 87
           F FP+T ST +PDP  FFSPNLLSSPLPTNSFFQNFVL +GD PEYIHPYLI++A SSLS
Sbjct: 61  FLFPKTQSTVLPDPSPFFSPNLLSSPLPTNSFFQNFVLKHGDQPEYIHPYLIKSALSSLS 120

Query: 88  VSYPSRISNSSITQLQFFPDLVISSPKKTFNTT---HFISSFSDLGVDLDI-GVFRFHLV 147
           + YP R SNS+     F  DL IS+ + T N T   + +SS+SDL V LD+ G  RF LV
Sbjct: 121 ICYPPRFSNSAFIYQIFNADLTISATRNTNNGTDVNNIVSSYSDLSVTLDVPGNLRFFLV 180

Query: 148 RGSPYLTFSVLKTSSVLISTSNGVRSVDSYEDYTKHIIRLNNGRSWVLYSSSAIYLVKSK 207
           RGSP+LT SV   + + IST + + S+ S  + TKHIIRLNN ++W+ YSSS I L  S 
Sbjct: 181 RGSPFLTCSVTSPTPLSISTIHAILSLTSNGNQTKHIIRLNNNQTWLCYSSSPINLTHSI 240

Query: 208 SNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCYPVSGFVKLLGGFGFEYKWQKKGS 267
           S  ++TS  F GVIR A+LPDS    E ILDR+S CYP SG       F  EYKW+KKG 
Sbjct: 241 S--LLTSDPFSGVIRFAILPDSDPRFETILDRFSTCYPTSGDAVFTKPFCVEYKWEKKGW 300

Query: 268 GGLLMLAHTLHREILPRDQ---TVLQNIRYSSIDGDLLGVVGDSWDLKFNPIPITWHSIN 327
           G LL+LAH LH+++L  D    TVL++ +Y SIDGDL+GVVGDSW L+ + + +TWHSIN
Sbjct: 301 GELLLLAHPLHQQLLSVDDGNVTVLKDFKYKSIDGDLVGVVGDSWVLRPSQVSVTWHSIN 360

Query: 328 GIDSKFFPEIVAALKRDVATLNATELSSMPASYFYGKLLARAARLALIAEEVNGGAGVIP 387
           GI  +++PEIV+AL +DV  L++T+ ++  +SYFYGKL+ARAARLALIAEEV     VIP
Sbjct: 361 GISEEYYPEIVSALVKDVKGLDSTKTTTS-SSYFYGKLIARAARLALIAEEVCC-PDVIP 420

Query: 388 AVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATSTTEDFGFGIYNDHHFHLGYF 447
           AV K+LK  I+PWL G F  NGF Y+ KW G+VTK G+  +  DFGFG+YNDHH+HLGYF
Sbjct: 421 AVRKYLKETIEPWLEGSFNGNGFFYDPKWGGIVTKQGSVDSGADFGFGVYNDHHYHLGYF 480

Query: 448 VYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKKSQFSIPFRNFDFWKLHSWAAGLTE 507
           +Y+IAVLAK+DP WG++Y+PQAY+L+ D+M    ++  +    RNFD WKLHSWA GLTE
Sbjct: 481 LYAIAVLAKIDPAWGRKYRPQAYSLMADFMTLGRREGSYYPRLRNFDLWKLHSWAGGLTE 540

Query: 508 FPDGRNQESTTEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEITATQTWWHVKRENNGI 567
           F DGRNQEST+EA+NAYY+AALMGL+Y D  L + GSTL   EI ++QTWWHV RE + +
Sbjct: 541 FADGRNQESTSEAINAYYSAALMGLSYGDSQLVSIGSTLATLEIQSSQTWWHV-REGDDM 600

Query: 568 YDKGFTEENRMVGILWSAARESRLWFAPAEWRECRVGIQVLPVLPVTERVFTDVGFVKEV 627
           Y+  FT ENR+VG+LW+  R+S LWFAP +W+ECR+GIQVLP+LP+TE +F+D+G V+++
Sbjct: 601 YEAEFTRENRVVGVLWANKRDSGLWFAPPDWKECRLGIQVLPLLPITEVLFSDIGHVRDL 660

Query: 628 VEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHDDGNSLSNLLWWIYSRP 683
           V+W  PALERE  GEGWKGF YA+EG+Y+K  A+EK+++L K DDGNSLSNLLWWI+SR 
Sbjct: 661 VQWTMPALEREGVGEGWKGFVYAMEGLYNKDGALEKIRRLNKFDDGNSLSNLLWWIHSRG 717

BLAST of Csa5G647400 vs. NCBI nr
Match: gi|703103793|ref|XP_010097822.1| (Endo-1,3(4)-beta-glucanase 1 [Morus notabilis])

HSP 1 Score: 788.1 bits (2034), Expect = 1.2e-224
Identity = 393/665 (59.10%), Postives = 490/665 (73.68%), Query Frame = 1

Query: 25  SAQFPFPETTSTAVPDPGKFFSPNLLSSPLPTNSFFQNFVLNNGDLPEYIHPYLIRTANS 84
           +A F FP+  ST V DP  FFSP+LLS+PLPTNSFFQNFVL NGD PEYIHPYL++++NS
Sbjct: 65  NAPFLFPKAQSTVVSDPSLFFSPDLLSTPLPTNSFFQNFVLKNGDQPEYIHPYLVKSSNS 124

Query: 85  SLSVSYPSRISNSSITQLQFFPDLVISSPKKTFNTT---HFISSFSDLGVDLDI--GVFR 144
           SLSV YPSR+SNS+     F  DL +S+ +KT   +   H ISSF+DL + LDI     R
Sbjct: 125 SLSVCYPSRLSNSAFLYQIFNADLTVSAAEKTAPDSQKPHLISSFNDLSLTLDIPSSNLR 184

Query: 145 FHLVRGSPYLTFSVLKTSSVLISTSNGVRSVDSYEDYTKHIIRLNNGRSWVLYSSSAIYL 204
           F LVRGSP+LT +    + + IST + + S  S    TK+ ++LNN ++W++Y+SS I  
Sbjct: 185 FFLVRGSPFLTCTASAKTEISISTIHAILSFSSNNSQTKYTVKLNNNQTWLIYTSSPISF 244

Query: 205 VKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCYPVSGFVKLLGGFGFEYKWQ 264
             S S  ++TS GF G++R+A+LPDS  E E ILDR+S CYPVSG V     F  EYKW+
Sbjct: 245 --SHSLSLLTSNGFSGILRIAILPDSNPEFEAILDRFSSCYPVSGEVSFTKPFCLEYKWE 304

Query: 265 KKGSGGLLMLAHTLHREILPRDQ---TVLQNIRYSSIDGDLLGVVGDSWDLKFNPIPITW 324
           KKG G LLMLAH LH ++L  D    TVL+  +Y SIDGDL+GVVGDSW LK +P+ +TW
Sbjct: 305 KKGWGDLLMLAHPLHLKLLKGDDRGVTVLEEFKYKSIDGDLVGVVGDSWTLKPDPVSVTW 364

Query: 325 HSINGIDSKFFPEIVAALKRDVATLNATELSSMPASYFYGKLLARAARLALIAEEVNGGA 384
           HSI G+  + + EIVAAL++DV  L+A  +++  ASYFYGKL+ARAAR ALIAEEV    
Sbjct: 365 HSIEGVKEEAYEEIVAALQKDVEALDAAAITTN-ASYFYGKLIARAARFALIAEEVRF-F 424

Query: 385 GVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATSTTEDFGFGIYNDHHFH 444
            VIP + KFLK  I+PWL G F  NGFLYE KW G++TK G      DFGFGIYNDHHFH
Sbjct: 425 DVIPKIRKFLKEAIEPWLDGTFNGNGFLYEEKWGGIITKQGQGDGGADFGFGIYNDHHFH 484

Query: 445 LGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKKSQFSIPFRNFDFWKLHSWAA 504
           LG+F+Y+IAVLAK+DP WG++YKPQAY+++ D+MN   +        R FD +KLHSWA 
Sbjct: 485 LGHFLYAIAVLAKIDPAWGRKYKPQAYSMMADFMNLSRRAKSNYPRLRCFDLYKLHSWAG 544

Query: 505 GLTEFPDGRNQESTTEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEITATQTWWHVKRE 564
           GLTEF DGRNQES++EAVNAYY+AALMGLAY D  L A GS LTA EI A Q WWHVK E
Sbjct: 545 GLTEFADGRNQESSSEAVNAYYSAALMGLAYGDTHLVAIGSLLTALEIQAAQMWWHVK-E 604

Query: 565 NNGIYDKGFTEENRMVGILWSAARESRLWFAPAEWRECRVGIQVLPVLPVTERVFTDVGF 624
            + +YD+ FT ENR+VG+LWS  R+S LWFAPA WRECR+GIQVLP+LP+TE +F+DVGF
Sbjct: 605 GDKLYDEDFTRENRVVGVLWSNKRDSGLWFAPAAWRECRLGIQVLPLLPITEVLFSDVGF 664

Query: 625 VKEVVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHDDGNSLSNLLWWI 682
            +E+V W  PAL+RE  GEGWKGF YALEGIYDK++A++K+K L   DDGNSL+NLLWWI
Sbjct: 665 TRELVNWTLPALQREGVGEGWKGFVYALEGIYDKETALQKIKSLNGFDDGNSLTNLLWWI 724